Prupe.8G150500_v2.0.a1

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0000910
Physical Location & Seq
Forward (+)
16387837 .. 16389806
1970 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G150500.1

Sequence Viewer

Length: 1494 bp
ATGAAACTGAGAGTGAGATCCATAGAAACCAAAGAGACCCTCCGAGTCGAGGTCCCCAATCTCTGCTCTCTCCACCAGCTTAAGCAAACCATCTCCCAATTGATCTCAGCCTCTCCTTCCTCTCTCCGCCTCTCCCTCAACCGACGCGACGAGCTCCACGCGTCCTCTCCTGACGACTCGCTTCACTCCCTCGGCCTCACCTCCGGTGATCTTATCTTCTACACCCTCGACCCCTCCTCCCAAACCCTAGCTCCACCTCCGCATTCAATTTCACATTCGTCCGTACAATCCCATAACCAAATCCTCACCACTGGTGTGCCTCAAGGTTCGAATTTCCAAAACCCTGAAACCCTAATTGGCGAAGGGTCTGCCGCGGGTGATTCGATTGCTGAAGAACAGAGGTCTCTGGTTTCGAATTCGGAAACCCTAGTGGCTTCTGGTGCTGAAAGAATGGAAATTGATGATGGGTCTGATGGGATAGGGTTGAAAAAGTACTCGGTGCCCTTTTTCTTGAAGAGGGTACTGAGGGAGGAGTTGGGAGAAGACCGTAGCAATCACAAGCTATTGGTGATTGCTGTTCATGCTGCGGTCTTGGAGTCCGGTTTCGTCGGGTTCGATTCCGTTTCGGGTATGGGGGCCAATCGGTTTCATCTCGCTGATGAATGGCCAAGGACAGCTATCACGATGTCGCTTTCCTATACTTTGCCTGAGATTCTGAAAAATCGTGGCAATAATGGTAATGGGGTTGAAGGGGTTATGTTGAAATTTCAGAGCTTGGGGCGTTTTGTTAATGTTTATGGGTCTTTGGCCAGTGGTGGTGCTGGGCCTTATCGGGTATGTTTGGACGAACACAGATTTGCCCCCATCATTGAATCTGTTTGGGAAAACAAGAATGTGAATGAGAGAGATGGGTTAGTCTCAGAAAGAGAGGTTTTTGAGTTTTGGAAGATTGTGAAGGATGGGATCACATTGCCATTGCTGATTGATCTTTGTGCAAAGGCCGGTTTGCCCGCCCCACCGAGCCTGATGCGCCTTCCACCGGAGCTCAAAATGAAGATTTTGGAGCCACTATCTGGTGTGGACATTGCCAAAGTGGGTGGTGTTTGTAAGGAGCTGCGAAATCTTGCTAATAATGATGAGTTGTGGAAGAAGAAGTATGCTGAGGAGTTTGGTAGTGGTACTGGAGGAGAAGGAACAATGATTAACTGGAAGCATAAGTTTGCTAGAAATTGGGAGATTGCGGAGCAACAAAGGAAGGCAGTAGGGTATTGGAGATCATATGAGAGGCCTTATTTCAACCGTATCAGGAGGGACCCTAACCCATTGTTTGTACCTCCAGTTCCTGGTATAATTGGTGGAGATTATGACCGCTTTCCGGTCTTTGGTGCCCTTAATCCTACTGGACAATCACACCCTATTCTACAACCACCCCGTCGGTTTCCAGCACGCCGTAATTTCTCACCAAATTGCAATCTGGAAGGGTTCCTTGGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like HLH

Protein Analysis

498

Amino Acids

54.95

Weight (kDa)

6.88

Isoelectric Point (pI)

50.47

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 499, 1385
AccB7I CCANNNNNTGG 2 cut(s) 1073, 1343
AccII CGCG 3 cut(s) 147, 161, 374
AciI CCGC 8 cut(s) 127, 260, 372, 374, 587, 1011, 1241, 1369
AclWI GGATC 2 cut(s) 12, 971
AcoI YGGCCR 2 cut(s) 665, 807
AcsI RAATTY 3 cut(s) 331, 415, 764
AcuI CTGAAG 1 cut(s) 411
AfaI GTAC 5 cut(s) 285, 494, 522, 1180, 1332
AfiI CCNNNNNNNGG 6 cut(s) 49, 1039, 1073, 1343, 1375, 1382
AflII CTTAAG 1 cut(s) 80
AflIII ACRYGT 1 cut(s) 159
AgsI TTSAA 7 cut(s) 267, 487, 514, 749, 763, 872, 1297
AjnI CCWGG 1 cut(s) 1342
AjuI GAANNNNNNNTTGG 3 cut(s) 339, 371, 1470
AleI CACNNNNGTG 1 cut(s) 314
AluBI AGCT 8 cut(s) 79, 154, 251, 562, 677, 774, 1045, 1114
AluI AGCT 8 cut(s) 79, 154, 251, 562, 677, 774, 1045, 1114
Alw21I GWGCWC 2 cut(s) 156, 1047
Alw26I GTCTC 3 cut(s) 29, 408, 922
AlwI GGATC 2 cut(s) 12, 971
AlwNI CAGNNNCTG 1 cut(s) 1343
AoxI GGCC 7 cut(s) 193, 636, 665, 807, 824, 999, 1286
ApeKI GCWGC 2 cut(s) 584, 1114
ApoI RAATTY 3 cut(s) 331, 415, 764
AspLEI GCGC 1 cut(s) 1032
AspS9I GGNCC 4 cut(s) 52, 636, 824, 1312
AsuHPI GGTGA 6 cut(s) 190, 218, 298, 389, 580, 1452
AsuII TTCGAA 2 cut(s) 329, 413
AvaII GGWCC 2 cut(s) 52, 1312
BaeGI GKGCMC 2 cut(s) 504, 1390
BalI TGGCCA 2 cut(s) 667, 809
BanI GGYRCC 2 cut(s) 499, 1385
BanII GRGCYC 2 cut(s) 156, 1047
BbsI GAAGAC 1 cut(s) 549
Bbv12I GWGCWC 2 cut(s) 156, 1047
BbvCI CCTCAGC 1 cut(s) 1161
BbvI GCAGC 2 cut(s) 571, 1101
BccI CCATC 6 cut(s) 98, 458, 467, 872, 902, 953
BceAI ACGGC 1 cut(s) 1434
BcgI CGANNNNNNTGC 4 cut(s) 350, 384, 1009, 1043
BciT130I CCWGG 1 cut(s) 1344
BcoDI GTCTC 3 cut(s) 29, 408, 922
BfaI CTAG 4 cut(s) 248, 428, 1224, 1492
BfrI CTTAAG 1 cut(s) 80
BisI GCNGC 3 cut(s) 372, 585, 1115
BlsI GCNGC 3 cut(s) 373, 586, 1116
BmcAI AGTACT 1 cut(s) 494
Bme1390I CCNGG 1 cut(s) 1344
Bme18I GGWCC 2 cut(s) 52, 1312
BmgT120I GGNCC 4 cut(s) 52, 636, 824, 1312
BmiI GGNNCC 8 cut(s) 54, 501, 637, 1065, 1313, 1314, 1387, 1484
BmrFI CCNGG 1 cut(s) 1344
BmsI GCATC 1 cut(s) 1017
BpiI GAAGAC 1 cut(s) 549
BpmI CTGGAG 2 cut(s) 1203, 1320
Bpu10I CCTNAGC 1 cut(s) 1161
Bpu14I TTCGAA 2 cut(s) 329, 413
BpuEI CTTGAG 1 cut(s) 306
BsaI GGTCTC 2 cut(s) 29, 408
BsaJI CCNNGG 4 cut(s) 190, 372, 668, 1486
BsaWI WCCGGW 4 cut(s) 203, 599, 1039, 1375
BsaXI ACNNNNNCTCC 4 cut(s) 221, 235, 251, 265
Bsc4I CCNNNNNNNGG 6 cut(s) 49, 1039, 1073, 1343, 1375, 1382
Bse118I RCCGGY 1 cut(s) 1001
Bse1I ACTGG 6 cut(s) 316, 810, 1186, 1211, 1337, 1405
Bse3DI GCAATG 3 cut(s) 968, 974, 1083
BseBI CCWGG 1 cut(s) 1344
BseDI CCNNGG 4 cut(s) 190, 372, 668, 1486
BseGI GGATG 1 cut(s) 964
BseLI CCNNNNNNNGG 6 cut(s) 49, 1039, 1073, 1343, 1375, 1382
BseMI GCAATG 3 cut(s) 968, 974, 1083
BseMII CTCAG 5 cut(s) 120, 515, 699, 933, 1152
BseNI ACTGG 6 cut(s) 316, 810, 1186, 1211, 1337, 1405
BseRI GAGGAG 4 cut(s) 226, 545, 1178, 1200
BseSI GKGCMC 2 cut(s) 504, 1390
BseXI GCAGC 2 cut(s) 571, 1101
BseYI CCCAGC 1 cut(s) 821
Bsh1236I CGCG 3 cut(s) 147, 161, 374
BshFI GGCC 7 cut(s) 195, 638, 667, 809, 826, 1001, 1288
BshNI GGYRCC 2 cut(s) 499, 1385
BsiHKAI GWGCWC 2 cut(s) 156, 1047
BsiSI CCGG 5 cut(s) 204, 600, 1002, 1040, 1376
BslFI GGGAC 2 cut(s) 38, 1325
BslI CCNNNNNNNGG 6 cut(s) 49, 1039, 1073, 1343, 1375, 1382
BsmAI GTCTC 3 cut(s) 29, 408, 922
BsmFI GGGAC 2 cut(s) 38, 1325
BsmI GAATGC 1 cut(s) 262
BsnI GGCC 7 cut(s) 195, 638, 667, 809, 826, 1001, 1288
Bso31I GGTCTC 2 cut(s) 29, 408
Bsp119I TTCGAA 2 cut(s) 329, 413
Bsp1286I GDGCHC 4 cut(s) 156, 504, 1047, 1390
Bsp143I GATC 6 cut(s) 17, 102, 208, 963, 985, 1274
BspACI CCGC 8 cut(s) 127, 260, 372, 374, 587, 1011, 1241, 1369
BspANI GGCC 7 cut(s) 195, 638, 667, 809, 826, 1001, 1288
BspCNI CTCAG 5 cut(s) 119, 516, 700, 932, 1153
BspFNI CGCG 3 cut(s) 147, 161, 374
BspLI GGNNCC 8 cut(s) 54, 501, 637, 1065, 1313, 1314, 1387, 1484
BspPI GGATC 2 cut(s) 12, 971
BspT104I TTCGAA 2 cut(s) 329, 413
BspT107I GGYRCC 2 cut(s) 499, 1385
BspTI CTTAAG 1 cut(s) 80
BspTNI GGTCTC 2 cut(s) 29, 408
BsrDI GCAATG 3 cut(s) 968, 974, 1083
BsrFI RCCGGY 1 cut(s) 1001
BsrI ACTGG 6 cut(s) 316, 810, 1186, 1211, 1337, 1405
BssAI RCCGGY 1 cut(s) 1001
BssECI CCNNGG 4 cut(s) 190, 372, 668, 1486
BssMI GATC 6 cut(s) 17, 102, 208, 963, 985, 1274
BssT1I CCWWGG 2 cut(s) 668, 1486
Bst2UI CCWGG 1 cut(s) 1344
Bst4CI ACNGT 2 cut(s) 548, 1301
Bst6I CTCTTC 1 cut(s) 509
BstAFI CTTAAG 1 cut(s) 80
BstBI TTCGAA 2 cut(s) 329, 413
BstC8I GCNNGC 2 cut(s) 1011, 1447
BstDEI CTNAG 6 cut(s) 8, 106, 524, 708, 919, 1161
BstDSI CCRYGG 1 cut(s) 372
BstF5I GGATG 1 cut(s) 964
BstFNI CGCG 3 cut(s) 147, 161, 374
BstHHI GCGC 1 cut(s) 1032
BstKTI GATC 6 cut(s) 20, 105, 211, 966, 988, 1277
BstMAI GTCTC 3 cut(s) 29, 408, 922
BstMBI GATC 6 cut(s) 17, 102, 208, 963, 985, 1274
BstMWI GCNNNNNNNGC 3 cut(s) 440, 581, 1029
BstNI CCWGG 1 cut(s) 1344
BstSCI CCNGG 1 cut(s) 1342
BstSLI GKGCMC 2 cut(s) 504, 1390
BstUI CGCG 3 cut(s) 147, 161, 374
BstV1I GCAGC 2 cut(s) 571, 1101
BstV2I GAAGAC 1 cut(s) 549
BstX2I RGATCY 1 cut(s) 17
BstYI RGATCY 1 cut(s) 17
BsuRI GGCC 7 cut(s) 195, 638, 667, 809, 826, 1001, 1288
BtgI CCRYGG 1 cut(s) 372
BtsCI GGATG 1 cut(s) 964
BtsIMutI CAGTG 2 cut(s) 309, 817
Cac8I GCNNGC 2 cut(s) 1011, 1447
CaiI CAGNNNCTG 1 cut(s) 1343
CfoI GCGC 1 cut(s) 1032
Cfr10I RCCGGY 1 cut(s) 1001
Cfr13I GGNCC 4 cut(s) 52, 636, 824, 1312
Cfr42I CCGCGG 1 cut(s) 375
CseI GACGC 2 cut(s) 150, 153
Csp6I GTAC 5 cut(s) 284, 493, 521, 1179, 1331
CspCI CAANNNNNGTGG 2 cut(s) 1078, 1113
CviAII CATG 1 cut(s) 581
CviQI GTAC 5 cut(s) 284, 493, 521, 1179, 1331
DdeI CTNAG 6 cut(s) 8, 106, 524, 708, 919, 1161
DpnI GATC 6 cut(s) 19, 104, 210, 965, 987, 1276
DpnII GATC 6 cut(s) 17, 102, 208, 963, 985, 1274
EaeI YGGCCR 2 cut(s) 665, 807
Eam1104I CTCTTC 1 cut(s) 509
EarI CTCTTC 1 cut(s) 509
EciI GGCGGA 1 cut(s) 116
Ecl136II GAGCTC 2 cut(s) 154, 1045
Eco130I CCWWGG 2 cut(s) 668, 1486
Eco147I AGGCCT 1 cut(s) 1288
Eco24I GRGCYC 2 cut(s) 156, 1047
Eco31I GGTCTC 2 cut(s) 29, 408
Eco47I GGWCC 2 cut(s) 52, 1312
Eco53kI GAGCTC 2 cut(s) 154, 1045
Eco57I CTGAAG 1 cut(s) 411
EcoICRI GAGCTC 2 cut(s) 154, 1045
EcoO109I RGGNCCY 2 cut(s) 52, 1312
EcoRI GAATTC 1 cut(s) 415
EcoRII CCWGG 1 cut(s) 1342
EcoT14I CCWWGG 2 cut(s) 668, 1486
EcoT38I GRGCYC 2 cut(s) 156, 1047
ErhI CCWWGG 2 cut(s) 668, 1486
FaeI CATG 1 cut(s) 584
FalI AAGNNNNNCTT 1 cut(s) 1470
FaqI GGGAC 2 cut(s) 38, 1325
FatI CATG 1 cut(s) 580
FauI CCCGC 2 cut(s) 367, 1018
FauNDI CATATG 1 cut(s) 1279
Fnu4HI GCNGC 3 cut(s) 372, 585, 1115
FokI GGATG 1 cut(s) 971
FriOI GRGCYC 2 cut(s) 156, 1047
Fsp4HI GCNGC 3 cut(s) 372, 585, 1115
FspBI CTAG 4 cut(s) 248, 428, 1224, 1492
GlaI GCGC 1 cut(s) 1031
GluI GCNGC 3 cut(s) 372, 585, 1115
GsaI CCCAGC 1 cut(s) 825
GsuI CTGGAG 2 cut(s) 1203, 1320
HaeIII GGCC 7 cut(s) 195, 638, 667, 809, 826, 1001, 1288
HapII CCGG 5 cut(s) 204, 600, 1002, 1040, 1376
HgaI GACGC 2 cut(s) 150, 153
HhaI GCGC 1 cut(s) 1032
Hin1II CATG 1 cut(s) 584
Hin6I GCGC 1 cut(s) 1030
HinP1I GCGC 1 cut(s) 1030
HinfI GANTC 7 cut(s) 45, 176, 380, 596, 617, 712, 872
HpaII CCGG 5 cut(s) 204, 600, 1002, 1040, 1376
HphI GGTGA 6 cut(s) 190, 218, 298, 389, 580, 1452
Hpy166II GTNNAC 1 cut(s) 1081
Hpy188I TCNGA 6 cut(s) 44, 421, 472, 717, 771, 922
Hpy188III TCNNGA 5 cut(s) 170, 511, 682, 1306, 1475
Hpy8I GTNNAC 1 cut(s) 1081
Hpy99I CGWCG 4 cut(s) 147, 152, 611, 1437
HpyAV CCTTC 8 cut(s) 126, 356, 743, 949, 1043, 1184, 1249, 1472
HpyCH4III ACNGT 2 cut(s) 548, 1301
HpyCH4V TGCA 2 cut(s) 995, 1470
HpyF10VI GCNNNNNNNGC 3 cut(s) 440, 581, 1029
HpyF3I CTNAG 6 cut(s) 8, 106, 524, 708, 919, 1161
Hsp92II CATG 1 cut(s) 584
HspAI GCGC 1 cut(s) 1030
KflI GGGWCCC 1 cut(s) 1312
KspI CCGCGG 1 cut(s) 375
Kzo9I GATC 6 cut(s) 17, 102, 208, 963, 985, 1274
LmnI GCTCC 6 cut(s) 159, 256, 1042, 1063, 1111, 1243
Lsp1109I GCAGC 2 cut(s) 571, 1101
LweI GCATC 1 cut(s) 1017
MaeI CTAG 4 cut(s) 248, 428, 1224, 1492
MalI GATC 6 cut(s) 19, 104, 210, 965, 987, 1276
MboI GATC 6 cut(s) 17, 102, 208, 963, 985, 1274
MboII GAAGA 8 cut(s) 208, 404, 526, 554, 958, 1066, 1159, 1162
MfeI CAATTG 1 cut(s) 98
MflI RGATCY 1 cut(s) 17
MhlI GDGCHC 4 cut(s) 156, 504, 1047, 1390
MlsI TGGCCA 2 cut(s) 667, 809
MluI ACGCGT 1 cut(s) 159
MluNI TGGCCA 2 cut(s) 667, 809
MlyI GAGTC 3 cut(s) 54, 170, 605
Mox20I TGGCCA 2 cut(s) 667, 809
MscI TGGCCA 2 cut(s) 667, 809
MseI TTAA 4 cut(s) 81, 789, 1203, 1392
MslI CAYNNNNRTG 1 cut(s) 314
Msp20I TGGCCA 2 cut(s) 667, 809
MspA1I CMGCKG 1 cut(s) 374
MspCI CTTAAG 1 cut(s) 80
MspI CCGG 5 cut(s) 204, 600, 1002, 1040, 1376
MspR9I CCNGG 1 cut(s) 1344
MunI CAATTG 1 cut(s) 98
Mva1269I GAATGC 1 cut(s) 262
MvaI CCWGG 1 cut(s) 1344
MvnI CGCG 3 cut(s) 147, 161, 374
MwoI GCNNNNNNNGC 3 cut(s) 440, 581, 1029
NdeI CATATG 1 cut(s) 1279
NdeII GATC 6 cut(s) 17, 102, 208, 963, 985, 1274
NlaIII CATG 1 cut(s) 584
NlaIV GGNNCC 8 cut(s) 54, 501, 637, 1065, 1313, 1314, 1387, 1484
NmeAIII GCCGAG 1 cut(s) 171
NspV TTCGAA 2 cut(s) 329, 413
OliI CACNNNNGTG 1 cut(s) 314
PceI AGGCCT 1 cut(s) 1288
PcsI WCGNNNNNNNCGW 1 cut(s) 612
PctI GAATGC 1 cut(s) 262
PfeI GAWTC 4 cut(s) 380, 617, 712, 872
PflMI CCANNNNNTGG 2 cut(s) 1073, 1343
PkrI GCNGC 3 cut(s) 373, 586, 1116
PleI GAGTC 3 cut(s) 53, 170, 604
PpsI GAGTC 3 cut(s) 53, 170, 604
PpuMI RGGWCCY 2 cut(s) 52, 1312
Psp124BI GAGCTC 2 cut(s) 156, 1047
Psp5II RGGWCCY 2 cut(s) 52, 1312
Psp6I CCWGG 1 cut(s) 1342
PspFI CCCAGC 1 cut(s) 821
PspGI CCWGG 1 cut(s) 1342
PspN4I GGNNCC 8 cut(s) 54, 501, 637, 1065, 1313, 1314, 1387, 1484
PspPI GGNCC 4 cut(s) 52, 636, 824, 1312
PspPPI RGGWCCY 2 cut(s) 52, 1312
PsrI GAACNNNNNNTAC 2 cut(s) 1323, 1355
PstNI CAGNNNCTG 1 cut(s) 1343
PsuI RGATCY 1 cut(s) 17
RsaI GTAC 5 cut(s) 285, 494, 522, 1180, 1332
RsaNI GTAC 5 cut(s) 284, 493, 521, 1179, 1331
RseI CAYNNNNRTG 1 cut(s) 314
SacI GAGCTC 2 cut(s) 156, 1047
SacII CCGCGG 1 cut(s) 375
SaqAI TTAA 4 cut(s) 81, 789, 1203, 1392
SatI GCNGC 3 cut(s) 372, 585, 1115
Sau3AI GATC 6 cut(s) 17, 102, 208, 963, 985, 1274
Sau96I GGNCC 4 cut(s) 52, 636, 824, 1312
ScaI AGTACT 1 cut(s) 494
SchI GAGTC 3 cut(s) 54, 170, 605
ScrFI CCNGG 1 cut(s) 1344
SduI GDGCHC 4 cut(s) 156, 504, 1047, 1390
SfaNI GCATC 1 cut(s) 1017
Sfr303I CCGCGG 1 cut(s) 375
SfuI TTCGAA 2 cut(s) 329, 413
SgrBI CCGCGG 1 cut(s) 375
SinI GGWCC 2 cut(s) 52, 1312
SmiMI CAYNNNNRTG 1 cut(s) 314
SmlI CTYRAG 2 cut(s) 80, 321
SmoI CTYRAG 2 cut(s) 80, 321
SseBI AGGCCT 1 cut(s) 1288
SsiI CCGC 8 cut(s) 127, 260, 372, 374, 587, 1011, 1241, 1369
SspMI CTAG 4 cut(s) 248, 428, 1224, 1492
SstI GAGCTC 2 cut(s) 156, 1047
StuI AGGCCT 1 cut(s) 1288
StyD4I CCNGG 1 cut(s) 1342
StyI CCWWGG 2 cut(s) 668, 1486
TaaI ACNGT 2 cut(s) 548, 1301
TaqI TCGA 6 cut(s) 48, 228, 329, 383, 413, 615
TatI WGTACW 1 cut(s) 492
TauI GCSGC 1 cut(s) 374
TfiI GAWTC 4 cut(s) 380, 617, 712, 872
Tru1I TTAA 4 cut(s) 81, 789, 1203, 1392
Tru9I TTAA 4 cut(s) 81, 789, 1203, 1392
TscAI CASTG 2 cut(s) 316, 817
TseI GCWGC 2 cut(s) 584, 1114
TspDTI ATGAA 5 cut(s) 17, 569, 638, 675, 1067
TspGWI ACGGA 2 cut(s) 271, 610
TspRI CASTG 2 cut(s) 316, 817
Van91I CCANNNNNTGG 2 cut(s) 1073, 1343
Vha464I CTTAAG 1 cut(s) 80
VpaK11BI GGWCC 2 cut(s) 52, 1312
XapI RAATTY 3 cut(s) 331, 415, 764
XspI CTAG 4 cut(s) 248, 428, 1224, 1492
ZrmI AGTACT 1 cut(s) 494
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.