MD10G1175800.v1.1

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0000910
Physical Location & Seq
Reverse (-)
26822580 .. 26823380
801 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1175800.v1.1.491

Sequence Viewer

Length: 801 bp
ATGGAAGAAATTGGTGGTGGGTCGGTGGCAAAGAAGATCATGTTCTTGAGGAGGGCGCTGACGGAGGAGTTGGCAGATGAGCGAAGTAATCACGAGCGGTTGGTGATCGCAGTCCATGCCGTGCTCTTAGAGTCTGGTTTCGTCCCAATCGATCCGATTTCAGGCAAGCAAACCGATGATCAGGATCGTCCTCATCTGTTGGATAAATGGTCAACACTGGATCAAAGTCAGACGATGTGGGTGTACTACACTCTCCCTCAGATTTTGCAAAACTGGACCAATATTGAAAAGTCTAAAGGTTGGACCATTACTGGAAATTATAACGTGATTGAAGGAGGAGGAGGAATTCAGTTGATGATTCAGAGTAGGGGTAATTTTGTCAATGTCTATCCGTCTTTGTCCGGTTACCGATTAGGGCTTTACTCACCTATCCATCACCTATGTTTGGATGAGCAAAGGTTTTGCCCCGCGATTGAATTTCTTTGGGCTAATAGGGATGTGGATCTTGGAGATAGGGATTTGCATCAGTACCAGTCCCCTGAAAAAGAAATTTTGGAATTGTGGAAGATTTCCATGGATGAGATTGTACTGCCTTTTCTGATTGAGCTTTGCCCGATGGCGGGTTTGCCGGCTCCAACGGTGTTTATGGACCTTCTGCCGGAGATAAAAAGGAAGATTTTCGAGTCACTTTCTGGTGTTGACATTTTAAAAGTGCCAGGTGTTTGTAAGAAGCTGAGAAATCTTGTTAATGATGATCAAGAGTTCTGGAAGCACAAGTTTCGTTCGAGAGTGGAAGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like HLH UN_NPL4

Protein Analysis

267

Amino Acids

30.51

Weight (kDa)

5.43

Isoelectric Point (pI)

58.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 220 - 261 2.1e-06 F-box-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 321
AccBSI CCGCTC 1 cut(s) 97
AccII CGCG 1 cut(s) 470
AciI CCGC 3 cut(s) 97, 468, 620
AclWI GGATC 4 cut(s) 146, 192, 228, 510
AcsI RAATTY 3 cut(s) 345, 476, 549
AfaI GTAC 3 cut(s) 245, 530, 588
AfiI CCNNNNNNNGG 5 cut(s) 161, 445, 619, 620, 658
AgsI TTSAA 3 cut(s) 287, 332, 476
AjnI CCWGG 1 cut(s) 715
AluBI AGCT 2 cut(s) 607, 733
AluI AGCT 2 cut(s) 607, 733
Alw21I GWGCWC 1 cut(s) 126
AlwI GGATC 4 cut(s) 146, 192, 228, 510
ApoI RAATTY 3 cut(s) 345, 476, 549
Asp700I GAANNNNTTC 1 cut(s) 677
AspLEI GCGC 1 cut(s) 58
AspS9I GGNCC 3 cut(s) 276, 303, 649
AsuHPI GGTGA 3 cut(s) 115, 417, 428
AvaII GGWCC 3 cut(s) 276, 303, 649
BauI CACGAG 1 cut(s) 92
Bbv12I GWGCWC 1 cut(s) 126
BccI CCATC 2 cut(s) 441, 610
BceAI ACGGC 1 cut(s) 104
BcgI CGANNNNNNTGC 2 cut(s) 761, 795
BciT130I CCWGG 1 cut(s) 717
BclI TGATCA 2 cut(s) 178, 754
BfoI RGCGCY 1 cut(s) 59
Bme1390I CCNGG 1 cut(s) 717
Bme18I GGWCC 3 cut(s) 276, 303, 649
BmgT120I GGNCC 3 cut(s) 276, 303, 649
BmiI GGNNCC 1 cut(s) 633
BmrFI CCNGG 1 cut(s) 717
BmsI GCATC 1 cut(s) 532
BpuEI CTTGAG 1 cut(s) 67
Bsa29I ATCGAT 1 cut(s) 150
BsaBI GATNNNNATC 3 cut(s) 183, 501, 522
BsaJI CCNNGG 1 cut(s) 573
BsaWI WCCGGW 1 cut(s) 401
Bsc4I CCNNNNNNNGG 5 cut(s) 161, 445, 619, 620, 658
Bse118I RCCGGY 1 cut(s) 628
Bse1I ACTGG 4 cut(s) 222, 278, 316, 532
Bse8I GATNNNNATC 3 cut(s) 183, 501, 522
BseBI CCWGG 1 cut(s) 717
BseCI ATCGAT 1 cut(s) 150
BseDI CCNNGG 1 cut(s) 573
BseGI GGATG 3 cut(s) 454, 502, 583
BseJI GATNNNNATC 3 cut(s) 183, 501, 522
BseLI CCNNNNNNNGG 5 cut(s) 161, 445, 619, 620, 658
BseMII CTCAG 2 cut(s) 272, 725
BseNI ACTGG 4 cut(s) 222, 278, 316, 532
BseRI GAGGAG 4 cut(s) 64, 80, 351, 354
Bsh1236I CGCG 1 cut(s) 470
BshVI ATCGAT 1 cut(s) 150
BsiHKAI GWGCWC 1 cut(s) 126
BsiSI CCGG 3 cut(s) 402, 629, 659
BslFI GGGAC 2 cut(s) 128, 520
BslI CCNNNNNNNGG 5 cut(s) 161, 445, 619, 620, 658
BsmFI GGGAC 2 cut(s) 128, 520
Bsp1286I GDGCHC 1 cut(s) 126
Bsp143I GATC 8 cut(s) 36, 105, 151, 178, 184, 220, 502, 754
Bsp19I CCATGG 1 cut(s) 573
BspACI CCGC 3 cut(s) 97, 468, 620
BspCNI CTCAG 2 cut(s) 271, 726
BspDI ATCGAT 1 cut(s) 150
BspFNI CGCG 1 cut(s) 470
BspLI GGNNCC 1 cut(s) 633
BspPI GGATC 4 cut(s) 146, 192, 228, 510
BsrBI CCGCTC 1 cut(s) 97
BsrFI RCCGGY 1 cut(s) 628
BsrI ACTGG 4 cut(s) 222, 278, 316, 532
BssAI RCCGGY 1 cut(s) 628
BssECI CCNNGG 1 cut(s) 573
BssMI GATC 8 cut(s) 36, 105, 151, 178, 184, 220, 502, 754
BssSI CACGAG 1 cut(s) 92
BssT1I CCWWGG 1 cut(s) 573
Bst2BI CACGAG 1 cut(s) 92
Bst2UI CCWGG 1 cut(s) 717
Bst4CI ACNGT 1 cut(s) 640
BstAPI GCANNNNNTGC 1 cut(s) 116
BstC8I GCNNGC 2 cut(s) 167, 630
BstDEI CTNAG 3 cut(s) 127, 258, 734
BstDSI CCRYGG 1 cut(s) 573
BstEII GGTNACC 1 cut(s) 404
BstF5I GGATG 3 cut(s) 454, 502, 583
BstFNI CGCG 1 cut(s) 470
BstH2I RGCGCY 1 cut(s) 59
BstHHI GCGC 1 cut(s) 58
BstKTI GATC 8 cut(s) 39, 108, 154, 181, 187, 223, 505, 757
BstMBI GATC 8 cut(s) 36, 105, 151, 178, 184, 220, 502, 754
BstMWI GCNNNNNNNGC 1 cut(s) 116
BstNI CCWGG 1 cut(s) 717
BstPI GGTNACC 1 cut(s) 404
BstSCI CCNGG 1 cut(s) 715
BstUI CGCG 1 cut(s) 470
BstX2I RGATCY 1 cut(s) 502
BstYI RGATCY 1 cut(s) 502
Bsu15I ATCGAT 1 cut(s) 150
BsuTUI ATCGAT 1 cut(s) 150
BtgI CCRYGG 1 cut(s) 573
BtsCI GGATG 3 cut(s) 454, 502, 583
BtsIMutI CAGTG 1 cut(s) 215
Cac8I GCNNGC 2 cut(s) 167, 630
CfoI GCGC 1 cut(s) 58
Cfr10I RCCGGY 1 cut(s) 628
Cfr13I GGNCC 3 cut(s) 276, 303, 649
ClaI ATCGAT 1 cut(s) 150
Csp6I GTAC 3 cut(s) 244, 529, 587
CviAII CATG 3 cut(s) 40, 116, 574
CviJI RGCY 6 cut(s) 418, 488, 607, 632, 733, 798
CviKI_1 RGCY 6 cut(s) 418, 488, 607, 632, 733, 798
CviQI GTAC 3 cut(s) 244, 529, 587
DdeI CTNAG 3 cut(s) 127, 258, 734
DpnI GATC 8 cut(s) 38, 107, 153, 180, 186, 222, 504, 756
DpnII GATC 8 cut(s) 36, 105, 151, 178, 184, 220, 502, 754
DraI TTTAAA 1 cut(s) 708
Eco130I CCWWGG 1 cut(s) 573
Eco47I GGWCC 3 cut(s) 276, 303, 649
Eco91I GGTNACC 1 cut(s) 404
EcoO65I GGTNACC 1 cut(s) 404
EcoRI GAATTC 1 cut(s) 345
EcoRII CCWGG 1 cut(s) 715
EcoT14I CCWWGG 1 cut(s) 573
ErhI CCWWGG 1 cut(s) 573
FaeI CATG 3 cut(s) 43, 119, 577
FaiI YATR 6 cut(s) 41, 117, 321, 442, 575, 647
FaqI GGGAC 2 cut(s) 128, 520
FatI CATG 3 cut(s) 39, 115, 573
FauI CCCGC 2 cut(s) 475, 613
FbaI TGATCA 2 cut(s) 178, 754
FokI GGATG 3 cut(s) 461, 509, 590
GlaI GCGC 1 cut(s) 57
HaeII RGCGCY 1 cut(s) 59
HapII CCGG 3 cut(s) 402, 629, 659
HhaI GCGC 1 cut(s) 58
Hin1II CATG 3 cut(s) 43, 119, 577
Hin6I GCGC 1 cut(s) 56
HinP1I GCGC 1 cut(s) 56
HincII GTYRAC 2 cut(s) 213, 700
HindII GTYRAC 2 cut(s) 213, 700
HinfI GANTC 3 cut(s) 131, 358, 683
HpaII CCGG 3 cut(s) 402, 629, 659
HphI GGTGA 3 cut(s) 115, 417, 428
Hpy166II GTNNAC 3 cut(s) 213, 244, 700
Hpy188I TCNGA 5 cut(s) 156, 231, 261, 363, 600
Hpy188III TCNNGA 6 cut(s) 46, 92, 182, 758, 766, 786
Hpy8I GTNNAC 3 cut(s) 213, 244, 700
HpyAV CCTTC 3 cut(s) 326, 662, 788
HpyCH4III ACNGT 1 cut(s) 640
HpyCH4IV ACGT 1 cut(s) 324
HpyCH4V TGCA 2 cut(s) 268, 523
HpyF10VI GCNNNNNNNGC 1 cut(s) 116
HpyF3I CTNAG 3 cut(s) 127, 258, 734
HpySE526I ACGT 1 cut(s) 324
Hsp92II CATG 3 cut(s) 43, 119, 577
HspAI GCGC 1 cut(s) 56
KroI GCCGGC 1 cut(s) 628
KroNI GCCGGC 1 cut(s) 630
Ksp22I TGATCA 2 cut(s) 178, 754
Kzo9I GATC 8 cut(s) 36, 105, 151, 178, 184, 220, 502, 754
LmnI GCTCC 1 cut(s) 637
LweI GCATC 1 cut(s) 532
MaeII ACGT 1 cut(s) 324
MaeIII GTNAC 2 cut(s) 404, 684
MalI GATC 8 cut(s) 38, 107, 153, 180, 186, 222, 504, 756
MbiI CCGCTC 1 cut(s) 97
MboI GATC 8 cut(s) 36, 105, 151, 178, 184, 220, 502, 754
MboII GAAGA 4 cut(s) 17, 46, 577, 685
MflI RGATCY 1 cut(s) 502
MhlI GDGCHC 1 cut(s) 126
MluCI AATT 7 cut(s) 9, 316, 345, 373, 476, 549, 557
MlyI GAGTC 2 cut(s) 140, 692
MmeI TCCRAC 3 cut(s) 180, 281, 659
MnlI CCTC 8 cut(s) 42, 45, 58, 201, 267, 329, 332, 335
MroNI GCCGGC 1 cut(s) 628
MroXI GAANNNNTTC 1 cut(s) 677
MseI TTAA 2 cut(s) 707, 747
MspI CCGG 3 cut(s) 402, 629, 659
MspR9I CCNGG 1 cut(s) 717
MvaI CCWGG 1 cut(s) 717
MvnI CGCG 1 cut(s) 470
MwoI GCNNNNNNNGC 1 cut(s) 116
NaeI GCCGGC 1 cut(s) 630
NcoI CCATGG 1 cut(s) 573
NdeII GATC 8 cut(s) 36, 105, 151, 178, 184, 220, 502, 754
NgoMIV GCCGGC 1 cut(s) 628
NlaIII CATG 3 cut(s) 43, 119, 577
NlaIV GGNNCC 1 cut(s) 633
NmuCI GTSAC 1 cut(s) 684
PcsI WCGNNNNNNNCGW 1 cut(s) 147
PdiI GCCGGC 1 cut(s) 630
PdmI GAANNNNTTC 1 cut(s) 677
PfeI GAWTC 1 cut(s) 358
PleI GAGTC 2 cut(s) 139, 691
PpsI GAGTC 2 cut(s) 139, 691
PsiI TTATAA 1 cut(s) 321
Psp6I CCWGG 1 cut(s) 715
PspEI GGTNACC 1 cut(s) 404
PspGI CCWGG 1 cut(s) 715
PspN4I GGNNCC 1 cut(s) 633
PspPI GGNCC 3 cut(s) 276, 303, 649
PsuI RGATCY 1 cut(s) 502
RsaI GTAC 3 cut(s) 245, 530, 588
RsaNI GTAC 3 cut(s) 244, 529, 587
SaqAI TTAA 2 cut(s) 707, 747
Sau3AI GATC 8 cut(s) 36, 105, 151, 178, 184, 220, 502, 754
Sau96I GGNCC 3 cut(s) 276, 303, 649
SchI GAGTC 2 cut(s) 140, 692
ScrFI CCNGG 1 cut(s) 717
SduI GDGCHC 1 cut(s) 126
SetI ASST 9 cut(s) 301, 327, 430, 441, 461, 609, 654, 721, 735
SfaNI GCATC 1 cut(s) 532
SinI GGWCC 3 cut(s) 276, 303, 649
SmlI CTYRAG 1 cut(s) 46
SmoI CTYRAG 1 cut(s) 46
Sse9I AATT 7 cut(s) 9, 316, 345, 373, 476, 549, 557
SsiI CCGC 3 cut(s) 97, 468, 620
SspI AATATT 1 cut(s) 283
StyD4I CCNGG 1 cut(s) 715
StyI CCWWGG 1 cut(s) 573
TaaI ACNGT 1 cut(s) 640
TaiI ACGT 1 cut(s) 327
TaqI TCGA 3 cut(s) 150, 681, 785
TasI AATT 7 cut(s) 9, 316, 345, 373, 476, 549, 557
TatI WGTACW 2 cut(s) 243, 586
TfiI GAWTC 1 cut(s) 358
Tru1I TTAA 2 cut(s) 707, 747
Tru9I TTAA 2 cut(s) 707, 747
TscAI CASTG 1 cut(s) 222
TseFI GTSAC 1 cut(s) 684
Tsp45I GTSAC 1 cut(s) 684
TspGWI ACGGA 2 cut(s) 77, 381
TspRI CASTG 1 cut(s) 222
VpaK11BI GGWCC 3 cut(s) 276, 303, 649
XapI RAATTY 3 cut(s) 345, 476, 549
XmnI GAANNNNTTC 1 cut(s) 677
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.