Rmu_sc0003402.1_g000015

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003402.1
OG0000910
Physical Location & Seq
Forward (+)
54671 .. 55959
1289 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003402.1_g000015.1.cds

Sequence Viewer

Length: 1206 bp
atggcgtttgaactaggacagcggacaccaatgggtgaagaagacgaagagagggcttgtgatcctttgaaagacgacaagtacaagtactccccatctttattgttgaggagggtactcacagaggagttggcagcccaagaccttcacagaaaccacaaaaacaacaagcttctcgtcgctgcggtgtatgctgtgctcttagactccggtttcgttcgctttagttcgggtacgtcagtacatgatgccctgttagagttaggtttcaccgagttcggttcggtatcgggtatgtcacattcctacactttgcctgtgattcttatcgaacaagataattgtgattgtagtagtaatcggaagatggaaggaattgttttgaagtttgagaattcagtacatgatgccctgttagagttaggtttcaccgagttcggttcggtatcgggtatgtcacattcctacactttgcctgagattcttatcgaacaagataattgtgattgtagtagtaatcggaagatggaaggaattgttttgaagtttgagaattcaggtaacttttcgtctgaccagaacagaacaaaacatgtggctcctcgcatggtgaaagttagtgggtccttgattaattctggttctaggtttgggcagaagcacaagaaggtatgtttggaattggatgagaataagtttgcgccggcgattgagtttttttggggaaataatacgaggaatactgtgaatgatttgggatttcgtgagagacaaatttgtgagttttggaaaattgtcaaggatgagatttcacttccattgctgattgatctttgtgcagaggccggtttgcctgctccattgtgccttatgagccttcctccggagctcaaaatgaagattttggtagcaatgccaggtgctgatcttgccagagtagggggtgtttgtaaggagctgcgagatcttgcaaatgacaatgagttgtggaagcacaagttagcccaggagtttgctgaggagttttctggtacaagagaagtagaagaactctggaaagttgcttttcgtcgacaatgggagactaaaagggagcaagcaaagaaactagtgggaaaaaagagaggacgtgagaacgtttattgtttccattggcaaccacgttctaaatatcctaaatggagtcatgaaccatatttcttgtag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like HLH UN_NPL4

Protein Analysis

401

Amino Acids

45.69

Weight (kDa)

6.17

Isoelectric Point (pI)

52.25

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 1072
AccIII TCCGGA 1 cut(s) 883
AciI CCGC 2 cut(s) 22, 185
AclI AACGTT 1 cut(s) 1137
AclWI GGATC 1 cut(s) 56
AcsI RAATTY 3 cut(s) 394, 553, 774
AfaI GTAC 7 cut(s) 83, 89, 117, 235, 243, 402, 1033
AfiI CCNNNNNNNGG 2 cut(s) 883, 939
AflIII ACRYGT 1 cut(s) 592
AgsI TTSAA 4 cut(s) 11, 70, 385, 544
AhlI ACTAGT 1 cut(s) 1108
AjiI CACGTC 1 cut(s) 1130
AjnI CCWGG 2 cut(s) 916, 1005
AjuI GAANNNNNNNTTGG 2 cut(s) 659, 691
AluBI AGCT 3 cut(s) 172, 889, 958
AluI AGCT 3 cut(s) 172, 889, 958
Alw21I GWGCWC 2 cut(s) 201, 891
Alw26I GTCTC 2 cut(s) 763, 1076
AlwI GGATC 1 cut(s) 56
AlwNI CAGNNNCTG 1 cut(s) 923
Aor13HI TCCGGA 1 cut(s) 883
AoxI GGCC 1 cut(s) 843
ApeKI GCWGC 3 cut(s) 134, 182, 958
ApoI RAATTY 3 cut(s) 394, 553, 774
ArsI GACNNNNNNTTYG 2 cut(s) 197, 229
AseI ATTAAT 1 cut(s) 633
AspLEI GCGC 1 cut(s) 703
AspS9I GGNCC 1 cut(s) 624
AsuHPI GGTGA 4 cut(s) 47, 262, 421, 622
AvaII GGWCC 1 cut(s) 624
BaeI ACNNNNGTAYC 2 cut(s) 225, 258
BanII GRGCYC 1 cut(s) 891
BbsI GAAGAC 1 cut(s) 48
Bbv12I GWGCWC 2 cut(s) 201, 891
BbvCI CCTCAGC 1 cut(s) 1017
BbvI GCAGC 3 cut(s) 146, 169, 945
BccI CCATC 3 cut(s) 103, 361, 520
BciT130I CCWGG 2 cut(s) 918, 1007
BcoDI GTCTC 2 cut(s) 763, 1076
BcuI ACTAGT 1 cut(s) 1108
BfaI CTAG 3 cut(s) 14, 645, 1109
BglII AGATCT 1 cut(s) 964
BisI GCNGC 3 cut(s) 135, 183, 959
BlsI GCNGC 3 cut(s) 136, 184, 960
BmcAI AGTACT 1 cut(s) 89
Bme1390I CCNGG 2 cut(s) 918, 1007
Bme18I GGWCC 1 cut(s) 624
BmgBI CACGTC 1 cut(s) 1130
BmgT120I GGNCC 1 cut(s) 624
BmiI GGNNCC 2 cut(s) 600, 625
BmrFI CCNGG 2 cut(s) 918, 1007
BmsI GCATC 2 cut(s) 238, 397
BpiI GAAGAC 1 cut(s) 48
BplI GAGNNNNNCTC 2 cut(s) 865, 897
Bpu10I CCTNAGC 1 cut(s) 1017
BsaBI GATNNNNATC 2 cut(s) 326, 485
BsaJI CCNNGG 1 cut(s) 1005
BsaWI WCCGGW 2 cut(s) 209, 883
BsaXI ACNNNNNCTCC 2 cut(s) 74, 104
Bsc4I CCNNNNNNNGG 2 cut(s) 883, 939
Bse118I RCCGGY 2 cut(s) 703, 845
Bse3DI GCAATG 2 cut(s) 818, 918
Bse8I GATNNNNATC 2 cut(s) 326, 485
BseAI TCCGGA 1 cut(s) 883
BseBI CCWGG 2 cut(s) 918, 1007
BseDI CCNNGG 1 cut(s) 1005
BseGI GGATG 2 cut(s) 691, 808
BseJI GATNNNNATC 2 cut(s) 326, 485
BseLI CCNNNNNNNGG 2 cut(s) 883, 939
BseMI GCAATG 2 cut(s) 818, 918
BseMII CTCAG 2 cut(s) 468, 1008
BseRI GAGGAG 4 cut(s) 124, 140, 591, 1034
BseXI GCAGC 3 cut(s) 146, 169, 945
BsgI GTGCAG 1 cut(s) 858
BshFI GGCC 1 cut(s) 845
BsiHKAI GWGCWC 2 cut(s) 201, 891
BsiSI CCGG 4 cut(s) 210, 704, 846, 884
BslI CCNNNNNNNGG 2 cut(s) 883, 939
BsmAI GTCTC 2 cut(s) 763, 1076
BsnI GGCC 1 cut(s) 845
Bsp1286I GDGCHC 2 cut(s) 201, 891
Bsp13I TCCGGA 1 cut(s) 883
Bsp143I GATC 4 cut(s) 61, 829, 925, 964
BspACI CCGC 2 cut(s) 22, 185
BspANI GGCC 1 cut(s) 845
BspCNI CTCAG 2 cut(s) 469, 1009
BspEI TCCGGA 1 cut(s) 883
BspHI TCATGA 1 cut(s) 1186
BspLI GGNNCC 2 cut(s) 600, 625
BspPI GGATC 1 cut(s) 56
BsrDI GCAATG 2 cut(s) 818, 918
BsrFI RCCGGY 2 cut(s) 703, 845
BssAI RCCGGY 2 cut(s) 703, 845
BssECI CCNNGG 1 cut(s) 1005
BssMI GATC 4 cut(s) 61, 829, 925, 964
Bst2UI CCWGG 2 cut(s) 918, 1007
Bst4CI ACNGT 1 cut(s) 745
Bst6I CTCTTC 1 cut(s) 42
BstC8I GCNNGC 3 cut(s) 705, 855, 1098
BstDEI CTNAG 3 cut(s) 202, 477, 1017
BstF5I GGATG 2 cut(s) 691, 808
BstHHI GCGC 1 cut(s) 703
BstKTI GATC 4 cut(s) 64, 832, 928, 967
BstMAI GTCTC 2 cut(s) 763, 1076
BstMBI GATC 4 cut(s) 61, 829, 925, 964
BstMWI GCNNNNNNNGC 3 cut(s) 191, 873, 929
BstNI CCWGG 2 cut(s) 918, 1007
BstNSI RCATGY 1 cut(s) 596
BstSCI CCNGG 2 cut(s) 916, 1005
BstV1I GCAGC 3 cut(s) 146, 169, 945
BstV2I GAAGAC 1 cut(s) 48
BstX2I RGATCY 1 cut(s) 964
BstYI RGATCY 1 cut(s) 964
BsuRI GGCC 1 cut(s) 845
BtrI CACGTC 1 cut(s) 1130
BtsCI GGATG 2 cut(s) 691, 808
Cac8I GCNNGC 3 cut(s) 705, 855, 1098
CaiI CAGNNNCTG 1 cut(s) 923
CciI TCATGA 1 cut(s) 1186
CfoI GCGC 1 cut(s) 703
Cfr10I RCCGGY 2 cut(s) 703, 845
Cfr13I GGNCC 1 cut(s) 624
Csp6I GTAC 7 cut(s) 82, 88, 116, 234, 242, 401, 1032
CspCI CAANNNNNGTGG 2 cut(s) 576, 611
CviAII CATG 5 cut(s) 245, 404, 593, 607, 1187
CviJI RGCY 9 cut(s) 56, 137, 172, 599, 845, 876, 889, 958, 1004
CviKI_1 RGCY 9 cut(s) 56, 137, 172, 599, 845, 876, 889, 958, 1004
CviQI GTAC 7 cut(s) 82, 88, 116, 234, 242, 401, 1032
DdeI CTNAG 3 cut(s) 202, 477, 1017
DpnI GATC 4 cut(s) 63, 831, 927, 966
DpnII GATC 4 cut(s) 61, 829, 925, 964
Eam1104I CTCTTC 1 cut(s) 42
EarI CTCTTC 1 cut(s) 42
Ecl136II GAGCTC 1 cut(s) 889
Eco24I GRGCYC 1 cut(s) 891
Eco47I GGWCC 1 cut(s) 624
Eco53kI GAGCTC 1 cut(s) 889
EcoICRI GAGCTC 1 cut(s) 889
EcoO109I RGGNCCY 1 cut(s) 624
EcoRI GAATTC 2 cut(s) 394, 553
EcoRII CCWGG 2 cut(s) 916, 1005
EcoT38I GRGCYC 1 cut(s) 891
FaeI CATG 5 cut(s) 248, 407, 596, 610, 1190
FatI CATG 5 cut(s) 244, 403, 592, 606, 1186
FblI GTMKAC 1 cut(s) 1072
Fnu4HI GCNGC 3 cut(s) 135, 183, 959
FokI GGATG 2 cut(s) 698, 815
FriOI GRGCYC 1 cut(s) 891
Fsp4HI GCNGC 3 cut(s) 135, 183, 959
FspBI CTAG 3 cut(s) 14, 645, 1109
GlaI GCGC 1 cut(s) 702
GluI GCNGC 3 cut(s) 135, 183, 959
HaeIII GGCC 1 cut(s) 845
HapII CCGG 4 cut(s) 210, 704, 846, 884
HhaI GCGC 1 cut(s) 703
Hin1II CATG 5 cut(s) 248, 407, 596, 610, 1190
Hin6I GCGC 1 cut(s) 701
HinP1I GCGC 1 cut(s) 701
HincII GTYRAC 1 cut(s) 1073
HindII GTYRAC 1 cut(s) 1073
HindIII AAGCTT 1 cut(s) 170
HinfI GANTC 4 cut(s) 206, 322, 481, 1183
HpaII CCGG 4 cut(s) 210, 704, 846, 884
HphI GGTGA 4 cut(s) 47, 262, 421, 622
Hpy166II GTNNAC 1 cut(s) 1073
Hpy188I TCNGA 3 cut(s) 363, 522, 574
Hpy188III TCNNGA 4 cut(s) 764, 884, 1054, 1187
Hpy8I GTNNAC 1 cut(s) 1073
Hpy99I CGWCG 2 cut(s) 182, 1074
HpyAV CCTTC 5 cut(s) 155, 365, 524, 661, 887
HpyCH4III ACNGT 1 cut(s) 745
HpyCH4IV ACGT 4 cut(s) 236, 1129, 1137, 1162
HpyCH4V TGCA 2 cut(s) 839, 971
HpyF10VI GCNNNNNNNGC 3 cut(s) 191, 873, 929
HpyF3I CTNAG 3 cut(s) 202, 477, 1017
HpySE526I ACGT 4 cut(s) 236, 1129, 1137, 1162
Hsp92II CATG 5 cut(s) 248, 407, 596, 610, 1190
HspAI GCGC 1 cut(s) 701
Kpn2I TCCGGA 1 cut(s) 883
KroI GCCGGC 1 cut(s) 703
KroNI GCCGGC 1 cut(s) 705
Kzo9I GATC 4 cut(s) 61, 829, 925, 964
LmnI GCTCC 5 cut(s) 604, 862, 886, 955, 1093
Lsp1109I GCAGC 3 cut(s) 146, 169, 945
LweI GCATC 2 cut(s) 238, 397
MaeI CTAG 3 cut(s) 14, 645, 1109
MaeII ACGT 4 cut(s) 236, 1129, 1137, 1162
MaeIII GTNAC 3 cut(s) 297, 456, 560
MalI GATC 4 cut(s) 63, 831, 927, 966
MboI GATC 4 cut(s) 61, 829, 925, 964
MboII GAAGA 7 cut(s) 50, 53, 59, 376, 535, 910, 1058
MflI RGATCY 1 cut(s) 964
MhlI GDGCHC 2 cut(s) 201, 891
MlyI GAGTC 2 cut(s) 200, 1192
MreI CGCCGGCG 1 cut(s) 703
MroI TCCGGA 1 cut(s) 883
MroNI GCCGGC 1 cut(s) 703
MseI TTAA 1 cut(s) 633
MspA1I CMGCKG 1 cut(s) 22
MspI CCGG 4 cut(s) 210, 704, 846, 884
MspR9I CCNGG 2 cut(s) 918, 1007
MvaI CCWGG 2 cut(s) 918, 1007
MwoI GCNNNNNNNGC 3 cut(s) 191, 873, 929
NaeI GCCGGC 1 cut(s) 705
NdeII GATC 4 cut(s) 61, 829, 925, 964
NgoMIV GCCGGC 1 cut(s) 703
NlaIII CATG 5 cut(s) 248, 407, 596, 610, 1190
NlaIV GGNNCC 2 cut(s) 600, 625
NmuCI GTSAC 2 cut(s) 297, 456
NspI RCATGY 1 cut(s) 596
PagI TCATGA 1 cut(s) 1186
PciI ACATGT 1 cut(s) 592
PdiI GCCGGC 1 cut(s) 705
PfeI GAWTC 2 cut(s) 322, 481
PkrI GCNGC 3 cut(s) 136, 184, 960
PleI GAGTC 2 cut(s) 200, 1191
PpsI GAGTC 2 cut(s) 200, 1191
PpuMI RGGWCCY 1 cut(s) 624
PscI ACATGT 1 cut(s) 592
PshBI ATTAAT 1 cut(s) 633
Psp124BI GAGCTC 1 cut(s) 891
Psp1406I AACGTT 1 cut(s) 1137
Psp5II RGGWCCY 1 cut(s) 624
Psp6I CCWGG 2 cut(s) 916, 1005
PspGI CCWGG 2 cut(s) 916, 1005
PspN4I GGNNCC 2 cut(s) 600, 625
PspPI GGNCC 1 cut(s) 624
PspPPI RGGWCCY 1 cut(s) 624
PstNI CAGNNNCTG 1 cut(s) 923
PsuI RGATCY 1 cut(s) 964
RsaI GTAC 7 cut(s) 83, 89, 117, 235, 243, 402, 1033
RsaNI GTAC 7 cut(s) 82, 88, 116, 234, 242, 401, 1032
SacI GAGCTC 1 cut(s) 891
SalI GTCGAC 1 cut(s) 1071
SaqAI TTAA 1 cut(s) 633
SatI GCNGC 3 cut(s) 135, 183, 959
Sau3AI GATC 4 cut(s) 61, 829, 925, 964
Sau96I GGNCC 1 cut(s) 624
ScaI AGTACT 1 cut(s) 89
SchI GAGTC 2 cut(s) 200, 1192
ScrFI CCNGG 2 cut(s) 918, 1007
SduI GDGCHC 2 cut(s) 201, 891
SfaNI GCATC 2 cut(s) 238, 397
SgrAI CRCCGGYG 1 cut(s) 703
SinI GGWCC 1 cut(s) 624
SpeI ACTAGT 1 cut(s) 1108
SsiI CCGC 2 cut(s) 22, 185
SspMI CTAG 3 cut(s) 14, 645, 1109
SstI GAGCTC 1 cut(s) 891
StyD4I CCNGG 2 cut(s) 916, 1005
TaaI ACNGT 1 cut(s) 745
TaiI ACGT 4 cut(s) 239, 1132, 1140, 1165
TaqI TCGA 3 cut(s) 330, 489, 1072
TatI WGTACW 4 cut(s) 81, 87, 241, 400
TfiI GAWTC 2 cut(s) 322, 481
Tru1I TTAA 1 cut(s) 633
Tru9I TTAA 1 cut(s) 633
TseFI GTSAC 2 cut(s) 297, 456
TseI GCWGC 3 cut(s) 134, 182, 958
Tsp45I GTSAC 2 cut(s) 297, 456
TspDTI ATGAA 2 cut(s) 911, 1203
VpaK11BI GGWCC 1 cut(s) 624
VspI ATTAAT 1 cut(s) 633
XapI RAATTY 3 cut(s) 394, 553, 774
XceI RCATGY 1 cut(s) 596
XmiI GTMKAC 1 cut(s) 1072
XspI CTAG 3 cut(s) 14, 645, 1109
ZrmI AGTACT 1 cut(s) 89
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.