Rmu_sc0002553.1_g000006

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002553.1
OG0000910
Physical Location & Seq
Reverse (-)
33307 .. 34845
1539 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002553.1_g000006.1.cds

Sequence Viewer

Length: 1539 bp
atgaagctgagactgcgatccatcgagtctaaagacactctccgaatagaggtccccaatctctgttctctccaccacctcaaacaatccctctccgaatccatctccgccgcctctccctcttctctccgcctctccctcaaccgccgcgacgagctcctctcctcctctcccgacgactccctcgcctccctcggcatcacctccggtgacctcatcttctacaccctcgacccctcctcccaaacccttcaatccccaattcctcacactccagtctccccaaatccccaaactctaaccctagttggccaaggggcttccacgagcgttgcaatggccgaagatccgaggtcgggttcggaaaccctaattggcgaagggtgttctcgtgtgggaacttcgatcgccgaagagccgaggtccgtggtttcgaattctgaaaccctaatggagtcggaaaccctaattggcgaagggtcttgtggtatggaaattgatgatgaagatgagtcgactggagtggtgaggaagaagtactctgttccgtttttcttgaggagggtgatgagggaggagttagccgaagatcgcagcgatcacaagctactggtgattgcattacatgcggtgctcgcagagtctcgattcgtcaggtttgattctgtttcgggtgtgcagaccgatcggtttcatcttccgaaagaatggccggcagtggcttacacgctgtccctcttgtatacattgcctgaggttctgcagaatcggggcaatggcggcggtggggttgaagctgtggtgctgaagattcagactttggggcattttgtcaatgtctatgggtcgttggccggtggcggcggttccgggcattatagggtgtctttggatgtgaagaggtttgcgccaatgctggattttgtctggggaagcaggaatgtgagtgatagtgaagtgtttgtcccggaaattgaaatctttgagttttggaggattgtcaaggatgggattacatacccattgctgattgatctttgtgcaaaggctggtttgcctgagccgcccagcttcatgtgccttcctttggatctcaagatgaatattttggagaggctgtctggtgttgacattgcgaaagtgggatgtgcgtgtaaggagctacaaaatctagccaataatgatgagctctggaagcagaagtttcaggaggagttcagagagggcgcaggaggaataggtaactggaagtttcgatatgcttggaagtgggaggatagggagcgagagaagaaggcaccggtcatgtggagaccaaggcttcatcacaatttccctattagaaggaacccagaaatcccatttgtacctggttttcctggaattattagggatcctccagattttggtgtccctgatccttttagacgaccacggttaccataccctcttggtggtggtcagcctgcaatcttttggcatgggaggaggcagaatttctctcccaattgcaatctgggaggaggatttagtgaatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like HLH

Protein Analysis

512

Amino Acids

56.89

Weight (kDa)

5.87

Isoelectric Point (pI)

61.53

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1297
AccB7I CCANNNNNTGG 1 cut(s) 1406
AccI GTMKAC 2 cut(s) 515, 743
AccII CGCG 1 cut(s) 150
AclWI GGATC 6 cut(s) 12, 341, 1098, 1388, 1401, 1412
AcoI YGGCCR 4 cut(s) 310, 339, 710, 852
AcsI RAATTY 2 cut(s) 436, 1495
AcuI CTGAAG 1 cut(s) 827
AfaI GTAC 2 cut(s) 539, 1368
AfiI CCNNNNNNNGG 5 cut(s) 49, 356, 1087, 1406, 1454
AgeI ACCGGT 1 cut(s) 1300
AgsI TTSAA 3 cut(s) 254, 794, 977
AjnI CCWGG 2 cut(s) 1369, 1378
AjuI GAANNNNNNNTTGG 4 cut(s) 357, 389, 453, 485
AluBI AGCT 7 cut(s) 7, 157, 607, 797, 1071, 1162, 1189
AluI AGCT 7 cut(s) 7, 157, 607, 797, 1071, 1162, 1189
Alw21I GWGCWC 3 cut(s) 159, 636, 1191
Alw26I GTCTC 4 cut(s) 4, 283, 648, 1306
AlwI GGATC 6 cut(s) 12, 341, 1098, 1388, 1401, 1412
AoxI GGCC 4 cut(s) 310, 339, 710, 852
ApeKI GCWGC 1 cut(s) 594
ApoI RAATTY 2 cut(s) 436, 1495
AsiGI ACCGGT 1 cut(s) 1300
AspLEI GCGC 2 cut(s) 910, 1229
AspS9I GGNCC 2 cut(s) 52, 423
AsuC2I CCSGG 2 cut(s) 871, 968
AsuHPI GGTGA 5 cut(s) 193, 221, 538, 577, 625
AsuII TTCGAA 1 cut(s) 434
AvaII GGWCC 2 cut(s) 52, 423
AxyI CCTNAGG 1 cut(s) 753
BalI TGGCCA 1 cut(s) 312
BamHI GGATCC 1 cut(s) 1393
BanI GGYRCC 1 cut(s) 1297
BanII GRGCYC 2 cut(s) 159, 1191
BauI CACGAG 2 cut(s) 325, 390
Bbv12I GWGCWC 3 cut(s) 159, 636, 1191
BbvI GCAGC 1 cut(s) 606
BccI CCATC 3 cut(s) 29, 110, 1001
BcgI CGANNNNNNTGC 2 cut(s) 668, 702
BciT130I CCWGG 2 cut(s) 1371, 1380
BcnI CCSGG 2 cut(s) 871, 968
BcoDI GTCTC 4 cut(s) 4, 283, 648, 1306
BfaI CTAG 2 cut(s) 305, 1172
BfmI CTRYAG 1 cut(s) 761
BisI GCNGC 6 cut(s) 111, 148, 595, 781, 862, 1064
BlsI GCNGC 6 cut(s) 112, 149, 596, 782, 863, 1065
BmcAI AGTACT 1 cut(s) 539
Bme1390I CCNGG 4 cut(s) 871, 968, 1371, 1380
Bme18I GGWCC 2 cut(s) 52, 423
BmgT120I GGNCC 2 cut(s) 52, 423
BmiI GGNNCC 5 cut(s) 54, 868, 1299, 1349, 1395
BmrFI CCNGG 4 cut(s) 871, 968, 1371, 1380
BmsI GCATC 1 cut(s) 207
BplI GAGNNNNNCTC 2 cut(s) 146, 178
BpmI CTGGAG 3 cut(s) 258, 540, 1383
Bpu10I CCTNAGC 1 cut(s) 1059
Bpu14I TTCGAA 1 cut(s) 434
BpuEI CTTGAG 2 cut(s) 577, 1079
BpuMI CCSGG 2 cut(s) 871, 968
BsaI GGTCTC 1 cut(s) 1306
BsaJI CCNNGG 7 cut(s) 193, 313, 350, 419, 426, 1316, 1433
BsaWI WCCGGW 2 cut(s) 206, 1300
BsaXI ACNNNNNCTCC 4 cut(s) 224, 254, 1103, 1133
Bsc4I CCNNNNNNNGG 5 cut(s) 49, 356, 1087, 1406, 1454
Bse118I RCCGGY 3 cut(s) 712, 854, 1300
Bse1I ACTGG 4 cut(s) 275, 523, 615, 1250
Bse21I CCTNAGG 1 cut(s) 753
Bse3DI GCAATG 5 cut(s) 342, 746, 781, 1022, 1131
BseBI CCWGG 2 cut(s) 1371, 1380
BseDI CCNNGG 7 cut(s) 193, 313, 350, 419, 426, 1316, 1433
BseGI GGATG 3 cut(s) 898, 1012, 1151
BseLI CCNNNNNNNGG 5 cut(s) 49, 356, 1087, 1406, 1454
BseMI GCAATG 5 cut(s) 342, 746, 781, 1022, 1131
BseMII CTCAG 2 cut(s) 744, 1050
BseNI ACTGG 4 cut(s) 275, 523, 615, 1250
BseRI GAGGAG 9 cut(s) 149, 154, 157, 229, 574, 590, 1226, 1501, 1536
BseXI GCAGC 1 cut(s) 606
BseYI CCCAGC 1 cut(s) 1067
BsgI GTGCAG 1 cut(s) 698
Bsh1236I CGCG 1 cut(s) 150
Bsh1285I CGRYCG 2 cut(s) 408, 688
BshFI GGCC 4 cut(s) 312, 341, 712, 854
BshNI GGYRCC 1 cut(s) 1297
BshTI ACCGGT 1 cut(s) 1300
BsiEI CGRYCG 2 cut(s) 408, 688
BsiHKAI GWGCWC 3 cut(s) 159, 636, 1191
BsiSI CCGG 6 cut(s) 207, 713, 855, 870, 968, 1301
BslFI GGGAC 4 cut(s) 38, 718, 950, 1397
BslI CCNNNNNNNGG 5 cut(s) 49, 356, 1087, 1406, 1454
BsmAI GTCTC 4 cut(s) 4, 283, 648, 1306
BsmFI GGGAC 4 cut(s) 38, 718, 950, 1397
BsnI GGCC 4 cut(s) 312, 341, 712, 854
Bso31I GGTCTC 1 cut(s) 1306
Bsp119I TTCGAA 1 cut(s) 434
Bsp1286I GDGCHC 3 cut(s) 159, 636, 1191
BspANI GGCC 4 cut(s) 312, 341, 712, 854
BspCNI CTCAG 2 cut(s) 745, 1051
BspFNI CGCG 1 cut(s) 150
BspLI GGNNCC 5 cut(s) 54, 868, 1299, 1349, 1395
BspMAI CTGCAG 1 cut(s) 765
BspPI GGATC 6 cut(s) 12, 341, 1098, 1388, 1401, 1412
BspQI GCTCTTC 1 cut(s) 408
BspT104I TTCGAA 1 cut(s) 434
BspT107I GGYRCC 1 cut(s) 1297
BspTNI GGTCTC 1 cut(s) 1306
BsrDI GCAATG 5 cut(s) 342, 746, 781, 1022, 1131
BsrFI RCCGGY 3 cut(s) 712, 854, 1300
BsrI ACTGG 4 cut(s) 275, 523, 615, 1250
BssAI RCCGGY 3 cut(s) 712, 854, 1300
BssECI CCNNGG 7 cut(s) 193, 313, 350, 419, 426, 1316, 1433
BssNAI GTATAC 1 cut(s) 744
BssSI CACGAG 2 cut(s) 325, 390
BssT1I CCWWGG 2 cut(s) 313, 1316
Bst1107I GTATAC 1 cut(s) 744
Bst2BI CACGAG 2 cut(s) 325, 390
Bst2UI CCWGG 2 cut(s) 1371, 1380
Bst4CI ACNGT 1 cut(s) 1437
Bst6I CTCTTC 3 cut(s) 127, 408, 893
BstAPI GCANNNNNTGC 1 cut(s) 626
BstBI TTCGAA 1 cut(s) 434
BstC8I GCNNGC 3 cut(s) 636, 714, 1467
BstDEI CTNAG 3 cut(s) 8, 753, 1059
BstDSI CCRYGG 2 cut(s) 426, 1433
BstEII GGTNACC 2 cut(s) 209, 1437
BstF5I GGATG 3 cut(s) 898, 1012, 1151
BstFNI CGCG 1 cut(s) 150
BstHHI GCGC 2 cut(s) 910, 1229
BstMAI GTCTC 4 cut(s) 4, 283, 648, 1306
BstMCI CGRYCG 2 cut(s) 408, 688
BstMWI GCNNNNNNNGC 7 cut(s) 13, 626, 635, 780, 1063, 1077, 1195
BstNI CCWGG 2 cut(s) 1371, 1380
BstNSI RCATGY 1 cut(s) 629
BstPI GGTNACC 2 cut(s) 209, 1437
BstSCI CCNGG 4 cut(s) 869, 966, 1369, 1378
BstSFI CTRYAG 1 cut(s) 761
BstUI CGCG 1 cut(s) 150
BstV1I GCAGC 1 cut(s) 606
BstX2I RGATCY 3 cut(s) 346, 1090, 1393
BstYI RGATCY 3 cut(s) 346, 1090, 1393
BstZ17I GTATAC 1 cut(s) 744
Bsu36I CCTNAGG 1 cut(s) 753
BsuRI GGCC 4 cut(s) 312, 341, 712, 854
BtgI CCRYGG 2 cut(s) 426, 1433
BtsCI GGATG 3 cut(s) 898, 1012, 1151
BtsI GCAGTG 1 cut(s) 723
BtsIMutI CAGTG 1 cut(s) 723
Cac8I GCNNGC 3 cut(s) 636, 714, 1467
CfoI GCGC 2 cut(s) 910, 1229
Cfr10I RCCGGY 3 cut(s) 712, 854, 1300
Cfr13I GGNCC 2 cut(s) 52, 423
CsiI ACCWGGT 1 cut(s) 1369
Csp6I GTAC 2 cut(s) 538, 1367
CspAI ACCGGT 1 cut(s) 1300
CspCI CAANNNNNGTGG 2 cut(s) 313, 348
CviAII CATG 4 cut(s) 626, 1075, 1306, 1481
CviQI GTAC 2 cut(s) 538, 1367
DdeI CTNAG 3 cut(s) 8, 753, 1059
EaeI YGGCCR 4 cut(s) 310, 339, 710, 852
Eam1104I CTCTTC 3 cut(s) 127, 408, 893
EarI CTCTTC 3 cut(s) 127, 408, 893
EciI GGCGGA 2 cut(s) 97, 119
Ecl136II GAGCTC 2 cut(s) 157, 1189
Eco130I CCWWGG 2 cut(s) 313, 1316
Eco24I GRGCYC 2 cut(s) 159, 1191
Eco31I GGTCTC 1 cut(s) 1306
Eco47I GGWCC 2 cut(s) 52, 423
Eco53kI GAGCTC 2 cut(s) 157, 1189
Eco57I CTGAAG 1 cut(s) 827
Eco81I CCTNAGG 1 cut(s) 753
Eco91I GGTNACC 2 cut(s) 209, 1437
EcoICRI GAGCTC 2 cut(s) 157, 1189
EcoO109I RGGNCCY 1 cut(s) 52
EcoO65I GGTNACC 2 cut(s) 209, 1437
EcoRI GAATTC 1 cut(s) 436
EcoRII CCWGG 2 cut(s) 1369, 1378
EcoT14I CCWWGG 2 cut(s) 313, 1316
EcoT38I GRGCYC 2 cut(s) 159, 1191
ErhI CCWWGG 2 cut(s) 313, 1316
FaeI CATG 4 cut(s) 629, 1078, 1309, 1484
FaqI GGGAC 4 cut(s) 38, 718, 950, 1397
FatI CATG 4 cut(s) 625, 1074, 1305, 1480
FblI GTMKAC 2 cut(s) 515, 743
Fnu4HI GCNGC 6 cut(s) 111, 148, 595, 781, 862, 1064
FokI GGATG 3 cut(s) 905, 1019, 1158
FriOI GRGCYC 2 cut(s) 159, 1191
Fsp4HI GCNGC 6 cut(s) 111, 148, 595, 781, 862, 1064
FspBI CTAG 2 cut(s) 305, 1172
GlaI GCGC 2 cut(s) 909, 1228
GluI GCNGC 6 cut(s) 111, 148, 595, 781, 862, 1064
GsaI CCCAGC 1 cut(s) 1071
GsuI CTGGAG 3 cut(s) 258, 540, 1383
HaeIII GGCC 4 cut(s) 312, 341, 712, 854
HapII CCGG 6 cut(s) 207, 713, 855, 870, 968, 1301
HhaI GCGC 2 cut(s) 910, 1229
Hin1II CATG 4 cut(s) 629, 1078, 1309, 1484
Hin6I GCGC 2 cut(s) 908, 1227
HinP1I GCGC 2 cut(s) 908, 1227
HincII GTYRAC 2 cut(s) 516, 1129
HindII GTYRAC 2 cut(s) 516, 1129
HpaII CCGG 6 cut(s) 207, 713, 855, 870, 968, 1301
HphI GGTGA 5 cut(s) 193, 221, 538, 577, 625
Hpy166II GTNNAC 3 cut(s) 516, 744, 1129
Hpy188I TCNGA 9 cut(s) 44, 97, 351, 364, 442, 460, 702, 816, 1220
Hpy188III TCNNGA 7 cut(s) 173, 556, 645, 1096, 1192, 1208, 1400
Hpy8I GTNNAC 3 cut(s) 516, 744, 1129
Hpy99I CGWCG 2 cut(s) 155, 179
HpyAV CCTTC 6 cut(s) 260, 374, 470, 1091, 1288, 1338
HpyCH4III ACNGT 1 cut(s) 1437
HpyCH4V TGCA 7 cut(s) 335, 620, 679, 763, 1043, 1469, 1512
HpyF10VI GCNNNNNNNGC 7 cut(s) 13, 626, 635, 780, 1063, 1077, 1195
HpyF3I CTNAG 3 cut(s) 8, 753, 1059
Hsp92II CATG 4 cut(s) 629, 1078, 1309, 1484
HspAI GCGC 2 cut(s) 908, 1227
KroI GCCGGC 1 cut(s) 712
KroNI GCCGGC 1 cut(s) 714
LguI GCTCTTC 1 cut(s) 408
LmnI GCTCC 3 cut(s) 162, 1159, 1282
Lsp1109I GCAGC 1 cut(s) 606
LweI GCATC 1 cut(s) 207
MabI ACCWGGT 1 cut(s) 1369
MaeI CTAG 2 cut(s) 305, 1172
MaeIII GTNAC 3 cut(s) 209, 1241, 1437
MfeI CAATTG 1 cut(s) 1507
MflI RGATCY 3 cut(s) 346, 1090, 1393
MhlI GDGCHC 3 cut(s) 159, 636, 1191
MlsI TGGCCA 1 cut(s) 312
MluNI TGGCCA 1 cut(s) 312
MlyI GAGTC 5 cut(s) 35, 173, 464, 521, 650
MmeI TCCRAC 1 cut(s) 438
Mox20I TGGCCA 1 cut(s) 312
MroNI GCCGGC 1 cut(s) 712
MscI TGGCCA 1 cut(s) 312
Msp20I TGGCCA 1 cut(s) 312
MspI CCGG 6 cut(s) 207, 713, 855, 870, 968, 1301
MspR9I CCNGG 4 cut(s) 871, 968, 1371, 1380
MunI CAATTG 1 cut(s) 1507
MvaI CCWGG 2 cut(s) 1371, 1380
MvnI CGCG 1 cut(s) 150
MwoI GCNNNNNNNGC 7 cut(s) 13, 626, 635, 780, 1063, 1077, 1195
NaeI GCCGGC 1 cut(s) 714
NciI CCSGG 2 cut(s) 871, 968
NgoMIV GCCGGC 1 cut(s) 712
NlaIII CATG 4 cut(s) 629, 1078, 1309, 1484
NlaIV GGNNCC 5 cut(s) 54, 868, 1299, 1349, 1395
NmeAIII GCCGAG 2 cut(s) 174, 444
NmuCI GTSAC 1 cut(s) 209
NspI RCATGY 1 cut(s) 629
NspV TTCGAA 1 cut(s) 434
PciSI GCTCTTC 1 cut(s) 408
PdiI GCCGGC 1 cut(s) 714
PfeI GAWTC 5 cut(s) 98, 648, 662, 766, 811
PflMI CCANNNNNTGG 1 cut(s) 1406
PfoI TCCNGGA 2 cut(s) 966, 1378
PinAI ACCGGT 1 cut(s) 1300
PkrI GCNGC 6 cut(s) 112, 149, 596, 782, 863, 1065
Ple19I CGATCG 2 cut(s) 408, 688
PleI GAGTC 5 cut(s) 34, 173, 463, 520, 649
PpsI GAGTC 5 cut(s) 34, 173, 463, 520, 649
PpuMI RGGWCCY 1 cut(s) 52
Psp124BI GAGCTC 2 cut(s) 159, 1191
Psp5II RGGWCCY 1 cut(s) 52
Psp6I CCWGG 2 cut(s) 1369, 1378
PspEI GGTNACC 2 cut(s) 209, 1437
PspFI CCCAGC 1 cut(s) 1067
PspGI CCWGG 2 cut(s) 1369, 1378
PspN4I GGNNCC 5 cut(s) 54, 868, 1299, 1349, 1395
PspPI GGNCC 2 cut(s) 52, 423
PspPPI RGGWCCY 1 cut(s) 52
PstI CTGCAG 1 cut(s) 765
PsuI RGATCY 3 cut(s) 346, 1090, 1393
PvuI CGATCG 2 cut(s) 408, 688
RsaI GTAC 2 cut(s) 539, 1368
RsaNI GTAC 2 cut(s) 538, 1367
SacI GAGCTC 2 cut(s) 159, 1191
SalI GTCGAC 1 cut(s) 514
SapI GCTCTTC 1 cut(s) 408
SatI GCNGC 6 cut(s) 111, 148, 595, 781, 862, 1064
Sau96I GGNCC 2 cut(s) 52, 423
ScaI AGTACT 1 cut(s) 539
SchI GAGTC 5 cut(s) 35, 173, 464, 521, 650
ScrFI CCNGG 4 cut(s) 871, 968, 1371, 1380
SduI GDGCHC 3 cut(s) 159, 636, 1191
SexAI ACCWGGT 1 cut(s) 1369
SfaNI GCATC 1 cut(s) 207
SfcI CTRYAG 1 cut(s) 761
SfuI TTCGAA 1 cut(s) 434
SinI GGWCC 2 cut(s) 52, 423
SmlI CTYRAG 2 cut(s) 556, 1094
SmoI CTYRAG 2 cut(s) 556, 1094
SspI AATATT 1 cut(s) 1105
SspMI CTAG 2 cut(s) 305, 1172
SstI GAGCTC 2 cut(s) 159, 1191
StyD4I CCNGG 4 cut(s) 869, 966, 1369, 1378
StyI CCWWGG 2 cut(s) 313, 1316
TaaI ACNGT 1 cut(s) 1437
TaqI TCGA 7 cut(s) 24, 231, 404, 434, 515, 646, 1255
TaqII GACCGA 1 cut(s) 698
TatI WGTACW 1 cut(s) 537
TauI GCSGC 5 cut(s) 113, 150, 783, 864, 1066
TfiI GAWTC 5 cut(s) 98, 648, 662, 766, 811
TscAI CASTG 1 cut(s) 723
TseFI GTSAC 1 cut(s) 209
TseI GCWGC 1 cut(s) 594
Tsp45I GTSAC 1 cut(s) 209
TspDTI ATGAA 6 cut(s) 17, 519, 683, 1063, 1115, 1313
TspGWI ACGGA 2 cut(s) 415, 537
TspRI CASTG 1 cut(s) 723
Van91I CCANNNNNTGG 1 cut(s) 1406
VpaK11BI GGWCC 2 cut(s) 52, 423
XapI RAATTY 2 cut(s) 436, 1495
XceI RCATGY 1 cut(s) 629
XcmI CCANNNNNNNNNTGG 1 cut(s) 1513
XmiI GTMKAC 2 cut(s) 515, 743
XspI CTAG 2 cut(s) 305, 1172
ZrmI AGTACT 1 cut(s) 539
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.