pycom05g10350

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0000910
Physical Location & Seq
Forward (+)
13726188 .. 13727651
1464 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g10350.3

Sequence Viewer

Length: 1380 bp
ATGAAACTGAGATTGCGATTGATAGAAACCAAAGAGACCATCCGAGTGGAGGTCCCCGATCTCTGCTCTTTCCACCACCTCAGACAAACCGTCTCCCAACTAATCTCTGCCGCCCCTTCCTCCCTCCGCCTCTCCCTCAACCGCCGCGACGAGCTCCACGCGCCATCCCCTCACGACCCCCTCCACTCCCTCGGCATCACCTCCGGCGACCTTGTCTTCTACACTCTCGACCCCTCCTCCCAAGCCCTAGCTTCGCCTTTCCATCCAATTTCGCATTCGTCCGCAGAATCCCCAAATCGAACTGAGCCCCAGTGTTCGAATTTCCTAAACTCCGAAACCCTAATTGACGAAGGGGCTGTTATGGCCGATTTAATTACTGAAAAACAGAGGTCTGGAGGTTCGAATTCGGAAACCGTAGTGGTTTCTGGTGCCGAATGTATGGATATTGATGACCCGTCTGATGTGCTAGGGTTGAAGAAGTACCCTGTTTCATTTTTCTTGAAGAGGGTACTGAGGGAGGAGTTGGGAGACGACCGTAGCAATCGCAGGCTATTGGTGATTGCTGTACAAGCTGTGCTCTTAGAGTCTGGTTTCGTTGGCTTCAATTCGTTTTCGGGTATGCTGGCCAATCAAAGGACGGGTTCAACGATTTCGCTTTCGTATACTCTGCCTGAGATATTGCGAAATTGGGGCAATACTGGTAGAGAGGTTGAAAGGGTTGTGTTGAAGTTTCAGAATTTGGGGCATTTTGTGAATGTTTATGGGTCTTTGGCAAATGACGGTTCTTCGCCCTATCAGGTATGTTTGGACAAAAACAGATTTGCCCCAACGATTAAATTTGTTTATGAAACCCAAAATGTCAATGACAGAGATGGGTTCGTTTTAGAGTTTCTGATCGATCTTTGCGCAAAGGCTGGTTTGCCTGCACCACCGAGCCTTATGTGCCTTCCACCGGAGCTCAAAATGAAGATTCTAGAGCCACTGGCTGGTGTTGACATTGCCAAGGTGGGTTGTGTTTGTAAGGAGCTGCAAAATCTTGGTAATAATGATGAGTTGTGGAAGCAGAAGTACGCTGAGGAGTTCGGTAATGGTAGCGGAGGAGAAGGAACTGGAGGTGAAGGAACAGTGACTAACTGGAAGTTTAGATTTGTTGGAAATTGGGAGACTAGGAAGCCTTATTTCAACCGTATCAGGAGGGACCCTAACCCATTTGTACCTCCAAGGATTGGTGGAATAGTTGGTGGAGATTATGACCGCTTTCCACTCTTTGGTGCAATTGATCCTACTGGACAAGCACACCCTCTTCTACAACAACCCCGAAGATCTGGTATGCGGCGTAATTTTTCACCCAATTGCGATCTGGGAGGGTTCAATATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like HLH

Protein Analysis

460

Amino Acids

50.78

Weight (kDa)

6.18

Isoelectric Point (pI)

47.64

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 907
AccB1I GGYRCC 1 cut(s) 428
AccB7I CCANNNNNTGG 3 cut(s) 986, 1226, 1268
AccI GTMKAC 1 cut(s) 662
AccII CGCG 2 cut(s) 147, 161
AciI CCGC 8 cut(s) 111, 127, 142, 145, 282, 1095, 1255, 1333
AclWI GGATC 1 cut(s) 1274
AcoI YGGCCR 2 cut(s) 363, 624
AcsI RAATTY 4 cut(s) 319, 403, 736, 836
AfaI GTAC 5 cut(s) 482, 510, 567, 1070, 1215
AfiI CCNNNNNNNGG 6 cut(s) 49, 633, 952, 986, 1226, 1268
AgsI TTSAA 8 cut(s) 475, 502, 604, 645, 713, 727, 1183, 1372
AhdI GACNNNNNGTC 1 cut(s) 89
AjuI GAANNNNNNNTTGG 2 cut(s) 259, 291
AluBI AGCT 5 cut(s) 154, 251, 572, 958, 1027
AluI AGCT 5 cut(s) 154, 251, 572, 958, 1027
Alw21I GWGCWC 3 cut(s) 156, 579, 960
Alw26I GTCTC 4 cut(s) 29, 97, 522, 1157
AlwI GGATC 1 cut(s) 1274
AoxI GGCC 2 cut(s) 363, 624
ApeKI GCWGC 1 cut(s) 1027
ApoI RAATTY 4 cut(s) 319, 403, 736, 836
AspLEI GCGC 2 cut(s) 163, 908
AspS9I GGNCC 2 cut(s) 52, 1198
AsuHPI GGTGA 4 cut(s) 190, 568, 1127, 1338
AsuII TTCGAA 2 cut(s) 317, 401
AvaII GGWCC 2 cut(s) 52, 1198
BalI TGGCCA 1 cut(s) 626
BanI GGYRCC 1 cut(s) 428
BanII GRGCYC 3 cut(s) 156, 309, 960
BarI GAAGNNNNNNTAC 2 cut(s) 1052, 1084
BbsI GAAGAC 1 cut(s) 208
Bbv12I GWGCWC 3 cut(s) 156, 579, 960
BbvCI CCTCAGC 1 cut(s) 1074
BbvI GCAGC 1 cut(s) 1014
BccI CCATC 4 cut(s) 47, 172, 270, 866
BcoDI GTCTC 4 cut(s) 29, 97, 522, 1157
BfaI CTAG 4 cut(s) 248, 467, 974, 1167
BglII AGATCT 1 cut(s) 1322
BisI GCNGC 4 cut(s) 111, 145, 1028, 1334
BlsI GCNGC 4 cut(s) 112, 146, 1029, 1335
Bme18I GGWCC 2 cut(s) 52, 1198
BmeRI GACNNNNNGTC 1 cut(s) 89
BmgT120I GGNCC 2 cut(s) 52, 1198
BmiI GGNNCC 4 cut(s) 54, 430, 1199, 1200
BmrI ACTGGG 1 cut(s) 304
BmsI GCATC 1 cut(s) 204
BmuI ACTGGG 1 cut(s) 304
BpiI GAAGAC 1 cut(s) 208
BpmI CTGGAG 2 cut(s) 414, 1131
Bpu10I CCTNAGC 1 cut(s) 1074
Bpu14I TTCGAA 2 cut(s) 317, 401
Bsa29I ATCGAT 1 cut(s) 897
BsaI GGTCTC 1 cut(s) 29
BsaJI CCNNGG 3 cut(s) 190, 1002, 1220
BsaWI WCCGGW 1 cut(s) 952
BsaXI ACNNNNNCTCC 2 cut(s) 221, 251
Bsc4I CCNNNNNNNGG 6 cut(s) 49, 633, 952, 986, 1226, 1268
Bse1I ACTGG 6 cut(s) 310, 703, 987, 1114, 1139, 1291
Bse3DI GCAATG 1 cut(s) 996
BseCI ATCGAT 1 cut(s) 897
BseDI CCNNGG 3 cut(s) 190, 1002, 1220
BseGI GGATG 3 cut(s) 39, 164, 262
BseLI CCNNNNNNNGG 6 cut(s) 49, 633, 952, 986, 1226, 1268
BseMI GCAATG 1 cut(s) 996
BseMII CTCAG 5 cut(s) 94, 294, 503, 663, 1065
BseNI ACTGG 6 cut(s) 310, 703, 987, 1114, 1139, 1291
BseRI GAGGAG 4 cut(s) 226, 533, 1091, 1113
BseXI GCAGC 1 cut(s) 1014
BsgI GTGCAG 1 cut(s) 909
Bsh1236I CGCG 2 cut(s) 147, 161
Bsh1285I CGRYCG 1 cut(s) 535
BshFI GGCC 2 cut(s) 365, 626
BshNI GGYRCC 1 cut(s) 428
BshVI ATCGAT 1 cut(s) 897
BsiEI CGRYCG 1 cut(s) 535
BsiHKAI GWGCWC 3 cut(s) 156, 579, 960
BsiSI CCGG 2 cut(s) 204, 953
BslFI GGGAC 2 cut(s) 38, 1211
BslI CCNNNNNNNGG 6 cut(s) 49, 633, 952, 986, 1226, 1268
BsmAI GTCTC 4 cut(s) 29, 97, 522, 1157
BsmBI CGTCTC 2 cut(s) 97, 522
BsmFI GGGAC 2 cut(s) 38, 1211
BsmI GAATGC 1 cut(s) 274
BsnI GGCC 2 cut(s) 365, 626
Bso31I GGTCTC 1 cut(s) 29
Bsp119I TTCGAA 2 cut(s) 317, 401
Bsp1286I GDGCHC 4 cut(s) 156, 309, 579, 960
Bsp1407I TGTACA 1 cut(s) 565
Bsp143I GATC 6 cut(s) 58, 894, 898, 1279, 1322, 1357
BspACI CCGC 8 cut(s) 111, 127, 142, 145, 282, 1095, 1255, 1333
BspANI GGCC 2 cut(s) 365, 626
BspCNI CTCAG 5 cut(s) 93, 295, 504, 664, 1066
BspDI ATCGAT 1 cut(s) 897
BspFNI CGCG 2 cut(s) 147, 161
BspLI GGNNCC 4 cut(s) 54, 430, 1199, 1200
BspPI GGATC 1 cut(s) 1274
BspT104I TTCGAA 2 cut(s) 317, 401
BspT107I GGYRCC 1 cut(s) 428
BspTNI GGTCTC 1 cut(s) 29
BsrDI GCAATG 1 cut(s) 996
BsrGI TGTACA 1 cut(s) 565
BsrI ACTGG 6 cut(s) 310, 703, 987, 1114, 1139, 1291
BssECI CCNNGG 3 cut(s) 190, 1002, 1220
BssMI GATC 6 cut(s) 58, 894, 898, 1279, 1322, 1357
BssNAI GTATAC 1 cut(s) 663
BssT1I CCWWGG 2 cut(s) 1002, 1220
Bst1107I GTATAC 1 cut(s) 663
Bst4CI ACNGT 6 cut(s) 91, 415, 536, 782, 1126, 1187
Bst6I CTCTTC 2 cut(s) 497, 1308
BstAUI TGTACA 1 cut(s) 565
BstBI TTCGAA 2 cut(s) 317, 401
BstC8I GCNNGC 3 cut(s) 548, 624, 924
BstDEI CTNAG 7 cut(s) 8, 80, 303, 512, 580, 672, 1074
BstF5I GGATG 3 cut(s) 39, 164, 262
BstFNI CGCG 2 cut(s) 147, 161
BstHHI GCGC 2 cut(s) 163, 908
BstKTI GATC 6 cut(s) 61, 897, 901, 1282, 1325, 1360
BstMAI GTCTC 4 cut(s) 29, 97, 522, 1157
BstMBI GATC 6 cut(s) 58, 894, 898, 1279, 1322, 1357
BstMCI CGRYCG 1 cut(s) 535
BstMWI GCNNNNNNNGC 4 cut(s) 160, 362, 569, 942
BstUI CGCG 2 cut(s) 147, 161
BstV1I GCAGC 1 cut(s) 1014
BstV2I GAAGAC 1 cut(s) 208
BstX2I RGATCY 1 cut(s) 1322
BstXI CCANNNNNNTGG 1 cut(s) 46
BstYI RGATCY 1 cut(s) 1322
BstZ17I GTATAC 1 cut(s) 663
Bsu15I ATCGAT 1 cut(s) 897
BsuRI GGCC 2 cut(s) 365, 626
BsuTUI ATCGAT 1 cut(s) 897
BtsCI GGATG 3 cut(s) 39, 164, 262
BtsIMutI CAGTG 3 cut(s) 317, 980, 1131
Cac8I GCNNGC 3 cut(s) 548, 624, 924
CfoI GCGC 2 cut(s) 163, 908
Cfr13I GGNCC 2 cut(s) 52, 1198
ClaI ATCGAT 1 cut(s) 897
Csp6I GTAC 5 cut(s) 481, 509, 566, 1069, 1214
CviQI GTAC 5 cut(s) 481, 509, 566, 1069, 1214
DdeI CTNAG 7 cut(s) 8, 80, 303, 512, 580, 672, 1074
DpnI GATC 6 cut(s) 60, 896, 900, 1281, 1324, 1359
DpnII GATC 6 cut(s) 58, 894, 898, 1279, 1322, 1357
DriI GACNNNNNGTC 1 cut(s) 89
EaeI YGGCCR 2 cut(s) 363, 624
Eam1104I CTCTTC 2 cut(s) 497, 1308
Eam1105I GACNNNNNGTC 1 cut(s) 89
EarI CTCTTC 2 cut(s) 497, 1308
EciI GGCGGA 1 cut(s) 116
Ecl136II GAGCTC 2 cut(s) 154, 958
Eco130I CCWWGG 2 cut(s) 1002, 1220
Eco24I GRGCYC 3 cut(s) 156, 309, 960
Eco31I GGTCTC 1 cut(s) 29
Eco47I GGWCC 2 cut(s) 52, 1198
Eco53kI GAGCTC 2 cut(s) 154, 958
EcoICRI GAGCTC 2 cut(s) 154, 958
EcoO109I RGGNCCY 2 cut(s) 52, 1198
EcoRI GAATTC 1 cut(s) 403
EcoT14I CCWWGG 2 cut(s) 1002, 1220
EcoT38I GRGCYC 3 cut(s) 156, 309, 960
ErhI CCWWGG 2 cut(s) 1002, 1220
Esp3I CGTCTC 2 cut(s) 97, 522
FaqI GGGAC 2 cut(s) 38, 1211
FblI GTMKAC 1 cut(s) 662
Fnu4HI GCNGC 4 cut(s) 111, 145, 1028, 1334
FokI GGATG 3 cut(s) 26, 151, 249
FriOI GRGCYC 3 cut(s) 156, 309, 960
Fsp4HI GCNGC 4 cut(s) 111, 145, 1028, 1334
FspBI CTAG 4 cut(s) 248, 467, 974, 1167
FspI TGCGCA 1 cut(s) 907
GlaI GCGC 2 cut(s) 162, 907
GluI GCNGC 4 cut(s) 111, 145, 1028, 1334
GsuI CTGGAG 2 cut(s) 414, 1131
HaeIII GGCC 2 cut(s) 365, 626
HapII CCGG 2 cut(s) 204, 953
HhaI GCGC 2 cut(s) 163, 908
Hin6I GCGC 2 cut(s) 161, 906
HinP1I GCGC 2 cut(s) 161, 906
HincII GTYRAC 1 cut(s) 994
HindII GTYRAC 1 cut(s) 994
HinfI GANTC 3 cut(s) 287, 584, 970
HpaII CCGG 2 cut(s) 204, 953
HphI GGTGA 4 cut(s) 190, 568, 1127, 1338
Hpy166II GTNNAC 2 cut(s) 663, 994
Hpy188I TCNGA 8 cut(s) 44, 83, 334, 409, 460, 735, 894, 1379
Hpy188III TCNNGA 6 cut(s) 173, 227, 393, 499, 974, 1192
Hpy8I GTNNAC 2 cut(s) 663, 994
Hpy99I CGWCG 1 cut(s) 152
HpyAV CCTTC 5 cut(s) 126, 344, 956, 1097, 1112
HpyCH4III ACNGT 6 cut(s) 91, 415, 536, 782, 1126, 1187
HpyCH4V TGCA 3 cut(s) 926, 1030, 1274
HpyF10VI GCNNNNNNNGC 4 cut(s) 160, 362, 569, 942
HpyF3I CTNAG 7 cut(s) 8, 80, 303, 512, 580, 672, 1074
HspAI GCGC 2 cut(s) 161, 906
KflI GGGWCCC 1 cut(s) 1198
Kzo9I GATC 6 cut(s) 58, 894, 898, 1279, 1322, 1357
LmnI GCTCC 3 cut(s) 159, 955, 1024
Lsp1109I GCAGC 1 cut(s) 1014
LweI GCATC 1 cut(s) 204
MaeI CTAG 4 cut(s) 248, 467, 974, 1167
MaeIII GTNAC 1 cut(s) 1126
MalI GATC 6 cut(s) 60, 896, 900, 1281, 1324, 1359
MboI GATC 6 cut(s) 58, 894, 898, 1279, 1322, 1357
MboII GAAGA 7 cut(s) 208, 487, 514, 777, 979, 1295, 1332
MfeI CAATTG 2 cut(s) 1275, 1351
MflI RGATCY 1 cut(s) 1322
MhlI GDGCHC 4 cut(s) 156, 309, 579, 960
MlsI TGGCCA 1 cut(s) 626
MluNI TGGCCA 1 cut(s) 626
MlyI GAGTC 1 cut(s) 593
MmeI TCCRAC 1 cut(s) 1132
Mox20I TGGCCA 1 cut(s) 626
MscI TGGCCA 1 cut(s) 626
MseI TTAA 2 cut(s) 371, 834
MslI CAYNNNNRTG 1 cut(s) 44
Msp20I TGGCCA 1 cut(s) 626
MspI CCGG 2 cut(s) 204, 953
MunI CAATTG 2 cut(s) 1275, 1351
Mva1269I GAATGC 1 cut(s) 274
MvnI CGCG 2 cut(s) 147, 161
MwoI GCNNNNNNNGC 4 cut(s) 160, 362, 569, 942
NdeII GATC 6 cut(s) 58, 894, 898, 1279, 1322, 1357
NlaIV GGNNCC 4 cut(s) 54, 430, 1199, 1200
NmeAIII GCCGAG 1 cut(s) 171
NmuCI GTSAC 1 cut(s) 1126
NsbI TGCGCA 1 cut(s) 907
NspV TTCGAA 2 cut(s) 317, 401
PcsI WCGNNNNNNNCGW 1 cut(s) 644
PctI GAATGC 1 cut(s) 274
PfeI GAWTC 2 cut(s) 287, 970
PflFI GACNNNGTC 1 cut(s) 212
PflMI CCANNNNNTGG 3 cut(s) 986, 1226, 1268
PkrI GCNGC 4 cut(s) 112, 146, 1029, 1335
PleI GAGTC 1 cut(s) 592
PpsI GAGTC 1 cut(s) 592
PpuMI RGGWCCY 2 cut(s) 52, 1198
Psp124BI GAGCTC 2 cut(s) 156, 960
Psp5II RGGWCCY 2 cut(s) 52, 1198
PspN4I GGNNCC 4 cut(s) 54, 430, 1199, 1200
PspPI GGNCC 2 cut(s) 52, 1198
PspPPI RGGWCCY 2 cut(s) 52, 1198
PsuI RGATCY 1 cut(s) 1322
PsyI GACNNNGTC 1 cut(s) 212
RsaI GTAC 5 cut(s) 482, 510, 567, 1070, 1215
RsaNI GTAC 5 cut(s) 481, 509, 566, 1069, 1214
RseI CAYNNNNRTG 1 cut(s) 44
SacI GAGCTC 2 cut(s) 156, 960
SaqAI TTAA 2 cut(s) 371, 834
SatI GCNGC 4 cut(s) 111, 145, 1028, 1334
Sau3AI GATC 6 cut(s) 58, 894, 898, 1279, 1322, 1357
Sau96I GGNCC 2 cut(s) 52, 1198
SchI GAGTC 1 cut(s) 593
SduI GDGCHC 4 cut(s) 156, 309, 579, 960
SfaNI GCATC 1 cut(s) 204
SfuI TTCGAA 2 cut(s) 317, 401
SinI GGWCC 2 cut(s) 52, 1198
SmiMI CAYNNNNRTG 1 cut(s) 44
SsiI CCGC 8 cut(s) 111, 127, 142, 145, 282, 1095, 1255, 1333
SspMI CTAG 4 cut(s) 248, 467, 974, 1167
SstI GAGCTC 2 cut(s) 156, 960
StyI CCWWGG 2 cut(s) 1002, 1220
TaaI ACNGT 6 cut(s) 91, 415, 536, 782, 1126, 1187
TaqI TCGA 5 cut(s) 228, 298, 317, 401, 897
TatI WGTACW 1 cut(s) 565
TauI GCSGC 3 cut(s) 113, 147, 1336
TfiI GAWTC 2 cut(s) 287, 970
Tru1I TTAA 2 cut(s) 371, 834
Tru9I TTAA 2 cut(s) 371, 834
TscAI CASTG 3 cut(s) 317, 987, 1131
TseFI GTSAC 1 cut(s) 1126
TseI GCWGC 1 cut(s) 1027
Tsp45I GTSAC 1 cut(s) 1126
TspDTI ATGAA 4 cut(s) 17, 480, 861, 980
TspRI CASTG 3 cut(s) 317, 987, 1131
Tth111I GACNNNGTC 1 cut(s) 212
Van91I CCANNNNNTGG 3 cut(s) 986, 1226, 1268
VpaK11BI GGWCC 2 cut(s) 52, 1198
XapI RAATTY 4 cut(s) 319, 403, 736, 836
XbaI TCTAGA 1 cut(s) 973
XcmI CCANNNNNNNNNTGG 1 cut(s) 1357
XmiI GTMKAC 1 cut(s) 662
XspI CTAG 4 cut(s) 248, 467, 974, 1167
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.