MD10G1110700.v1.1

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0000910
Physical Location & Seq
Forward (+)
18220429 .. 18221529
1101 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1110700.v1.1.491

Sequence Viewer

Length: 1041 bp
ATGCAATTACAGATCATAACACTCAACCACCGCGACGAGCTCCAAGAGCCATCCCCTAACAACTCCCTCCGCTCCCTCGGCATCACCTCCGGCGATCTTGTCTTCTGCACCCTCGAACCTTCCTTCCAAACCCTAGCTTTGCCTTCCCATCCAAATTTGCACTCATCCGCAGAATCCCCAAATCGAATTGAGCCCCAATGTTCGAATTTCCGTAACTCCGAAACCCTAATTGACAAAGGGGGTGTTATGGACGATTCGATTGGTGAAGATCCGAAGTCTGGAGGTTCGAATTCGAAAACCCTGGTAGTTTCTGGTGCCGAAAGTATGGAAATTGATGATCCGTCTGATGGGCTAGGGTTGGAAAGGTACTTGGTTCCATTTTTCTTAAAGCGGGTACTGAGGGAAGAGTTGGGAGAGGATCATAGCAATCATCACAAGCTATTGTTGATTGCTGTACATGTTGTGCTATTAGAGTTTGGTTTCGTTGGGTTCGATTCACTTTCGAGTATGTTGGCCAATCAAAAGACGGGTTCAACAATTTCGCTTTCATATACTTTGCCTGAGCTTTTGCAAAATCTGGGCAGTGATCGTAATGGGGTTGAAGGGGTTGTGTTGAAGTTTCAGAATTTGGGGCATTTTGTGAATGTTTATGGGTCTTTGGCCTTTGGAGGTTCTTCGCCCCATCAGATATGTTTGGACAAAAACAGATTTGCCCCAATGATCGAATCCATTTGGGAAAACCAAAATGTGAATGAGAGAGATGGGTTAGCCCCAGAGAGAGAAGTTTTAGAGTTTTGGAAGATTGTGAAAAATGGGATCACATTTCCATTGCTGATTGATCTTTGCGCAATGGCGGGTTTGCCCGCCCCACCGAGCCTTATACGCCTTCCACCGGAGCTCAAAATGAAGATTCTGGAGCCACTGCCTGGTGTCGACATTTCCAAGAAGTATGCTGAGGAGTTCGGTAATGGCAGCAGAGGAGAAGGAACTGGAGGTGAAGGAACAGTGACTAACTGGAAGTTTAGGTTTGTTGGAAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like HLH

Protein Analysis

347

Amino Acids

37.92

Weight (kDa)

5.06

Isoelectric Point (pI)

42.47

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 847
AccB1I GGYRCC 1 cut(s) 314
AccB7I CCANNNNNTGG 1 cut(s) 926
AccBSI CCGCTC 1 cut(s) 72
AccI GTMKAC 1 cut(s) 933
AccII CGCG 1 cut(s) 33
AciI CCGC 6 cut(s) 31, 70, 168, 391, 854, 864
AclWI GGATC 4 cut(s) 263, 332, 426, 824
AcoI YGGCCR 1 cut(s) 513
AcsI RAATTY 4 cut(s) 154, 205, 289, 625
AfaI GTAC 3 cut(s) 368, 396, 456
AfiI CCNNNNNNNGG 4 cut(s) 278, 347, 892, 926
AflIII ACRYGT 1 cut(s) 457
AgsI TTSAA 3 cut(s) 534, 602, 616
AjnI CCWGG 2 cut(s) 300, 925
AloI GAACNNNNNNTCC 2 cut(s) 108, 140
AluBI AGCT 5 cut(s) 40, 137, 439, 565, 898
AluI AGCT 5 cut(s) 40, 137, 439, 565, 898
Alw21I GWGCWC 2 cut(s) 42, 900
AlwI GGATC 4 cut(s) 263, 332, 426, 824
AoxI GGCC 2 cut(s) 513, 660
ApeKI GCWGC 1 cut(s) 972
ApoI RAATTY 4 cut(s) 154, 205, 289, 625
AspLEI GCGC 1 cut(s) 848
AsuHPI GGTGA 3 cut(s) 76, 275, 1007
AsuII TTCGAA 3 cut(s) 203, 287, 293
BalI TGGCCA 1 cut(s) 515
BanI GGYRCC 1 cut(s) 314
BanII GRGCYC 3 cut(s) 42, 195, 900
BbsI GAAGAC 1 cut(s) 94
Bbv12I GWGCWC 2 cut(s) 42, 900
BbvCI CCTCAGC 1 cut(s) 954
BbvI GCAGC 1 cut(s) 984
BccI CCATC 5 cut(s) 58, 156, 341, 690, 755
BciT130I CCWGG 2 cut(s) 302, 927
BfaI CTAG 2 cut(s) 134, 353
BisI GCNGC 1 cut(s) 973
BlsI GCNGC 1 cut(s) 974
Bme1390I CCNGG 2 cut(s) 302, 927
BmiI GGNNCC 3 cut(s) 316, 375, 918
BmrFI CCNGG 2 cut(s) 302, 927
BmsI GCATC 1 cut(s) 90
BpiI GAAGAC 1 cut(s) 94
BpmI CTGGAG 3 cut(s) 300, 935, 1011
Bpu10I CCTNAGC 2 cut(s) 561, 954
Bpu14I TTCGAA 3 cut(s) 203, 287, 293
BsaJI CCNNGG 2 cut(s) 76, 300
BsaWI WCCGGW 1 cut(s) 892
Bsc4I CCNNNNNNNGG 4 cut(s) 278, 347, 892, 926
Bse1I ACTGG 2 cut(s) 994, 1019
Bse3DI GCAATG 2 cut(s) 827, 855
BseBI CCWGG 2 cut(s) 302, 927
BseDI CCNNGG 2 cut(s) 76, 300
BseGI GGATG 3 cut(s) 50, 148, 164
BseLI CCNNNNNNNGG 4 cut(s) 278, 347, 892, 926
BseMI GCAATG 2 cut(s) 827, 855
BseMII CTCAG 3 cut(s) 389, 552, 945
BseNI ACTGG 2 cut(s) 994, 1019
BseRI GAGGAG 2 cut(s) 971, 993
BseXI GCAGC 1 cut(s) 984
BsgI GTGCAG 1 cut(s) 91
Bsh1236I CGCG 1 cut(s) 33
BshFI GGCC 2 cut(s) 515, 662
BshNI GGYRCC 1 cut(s) 314
BsiHKAI GWGCWC 2 cut(s) 42, 900
BsiSI CCGG 2 cut(s) 90, 893
BslI CCNNNNNNNGG 4 cut(s) 278, 347, 892, 926
BsnI GGCC 2 cut(s) 515, 662
Bsp119I TTCGAA 3 cut(s) 203, 287, 293
Bsp1286I GDGCHC 3 cut(s) 42, 195, 900
Bsp1407I TGTACA 1 cut(s) 454
Bsp143I GATC 9 cut(s) 12, 94, 268, 337, 418, 586, 720, 816, 838
BspACI CCGC 6 cut(s) 31, 70, 168, 391, 854, 864
BspANI GGCC 2 cut(s) 515, 662
BspCNI CTCAG 3 cut(s) 390, 553, 946
BspFNI CGCG 1 cut(s) 33
BspLI GGNNCC 3 cut(s) 316, 375, 918
BspPI GGATC 4 cut(s) 263, 332, 426, 824
BspT104I TTCGAA 3 cut(s) 203, 287, 293
BspT107I GGYRCC 1 cut(s) 314
BsrBI CCGCTC 1 cut(s) 72
BsrDI GCAATG 2 cut(s) 827, 855
BsrGI TGTACA 1 cut(s) 454
BsrI ACTGG 2 cut(s) 994, 1019
BssECI CCNNGG 2 cut(s) 76, 300
BssMI GATC 9 cut(s) 12, 94, 268, 337, 418, 586, 720, 816, 838
Bst2UI CCWGG 2 cut(s) 302, 927
Bst4CI ACNGT 1 cut(s) 1006
Bst6I CTCTTC 1 cut(s) 399
BstAUI TGTACA 1 cut(s) 454
BstBI TTCGAA 3 cut(s) 203, 287, 293
BstC8I GCNNGC 1 cut(s) 864
BstDEI CTNAG 3 cut(s) 398, 561, 954
BstF5I GGATG 3 cut(s) 50, 148, 164
BstFNI CGCG 1 cut(s) 33
BstHHI GCGC 1 cut(s) 848
BstKTI GATC 9 cut(s) 15, 97, 271, 340, 421, 589, 723, 819, 841
BstMBI GATC 9 cut(s) 12, 94, 268, 337, 418, 586, 720, 816, 838
BstMWI GCNNNNNNNGC 3 cut(s) 46, 78, 882
BstNI CCWGG 2 cut(s) 302, 927
BstNSI RCATGY 1 cut(s) 461
BstSCI CCNGG 2 cut(s) 300, 925
BstUI CGCG 1 cut(s) 33
BstV1I GCAGC 1 cut(s) 984
BstV2I GAAGAC 1 cut(s) 94
BstX2I RGATCY 1 cut(s) 268
BstYI RGATCY 1 cut(s) 268
BsuRI GGCC 2 cut(s) 515, 662
BtsCI GGATG 3 cut(s) 50, 148, 164
BtsI GCAGTG 2 cut(s) 589, 920
BtsIMutI CAGTG 3 cut(s) 589, 920, 1011
Cac8I GCNNGC 1 cut(s) 864
CfoI GCGC 1 cut(s) 848
Csp6I GTAC 3 cut(s) 367, 395, 455
CviAII CATG 1 cut(s) 458
CviQI GTAC 3 cut(s) 367, 395, 455
DdeI CTNAG 3 cut(s) 398, 561, 954
DpnI GATC 9 cut(s) 14, 96, 270, 339, 420, 588, 722, 818, 840
DpnII GATC 9 cut(s) 12, 94, 268, 337, 418, 586, 720, 816, 838
EaeI YGGCCR 1 cut(s) 513
Eam1104I CTCTTC 1 cut(s) 399
EarI CTCTTC 1 cut(s) 399
Ecl136II GAGCTC 2 cut(s) 40, 898
Eco24I GRGCYC 3 cut(s) 42, 195, 900
Eco53kI GAGCTC 2 cut(s) 40, 898
EcoICRI GAGCTC 2 cut(s) 40, 898
EcoRI GAATTC 1 cut(s) 289
EcoRII CCWGG 2 cut(s) 300, 925
EcoT38I GRGCYC 3 cut(s) 42, 195, 900
FaeI CATG 1 cut(s) 461
FatI CATG 1 cut(s) 457
FauI CCCGC 3 cut(s) 384, 847, 871
FblI GTMKAC 1 cut(s) 933
Fnu4HI GCNGC 1 cut(s) 973
FokI GGATG 3 cut(s) 37, 135, 151
FriOI GRGCYC 3 cut(s) 42, 195, 900
Fsp4HI GCNGC 1 cut(s) 973
FspBI CTAG 2 cut(s) 134, 353
FspI TGCGCA 1 cut(s) 847
GlaI GCGC 1 cut(s) 847
GluI GCNGC 1 cut(s) 973
GsuI CTGGAG 3 cut(s) 300, 935, 1011
HaeIII GGCC 2 cut(s) 515, 662
HapII CCGG 2 cut(s) 90, 893
HhaI GCGC 1 cut(s) 848
Hin1II CATG 1 cut(s) 461
Hin6I GCGC 1 cut(s) 846
HinP1I GCGC 1 cut(s) 846
HincII GTYRAC 1 cut(s) 934
HindII GTYRAC 1 cut(s) 934
HinfI GANTC 5 cut(s) 173, 254, 494, 725, 910
HpaII CCGG 2 cut(s) 90, 893
HphI GGTGA 3 cut(s) 76, 275, 1007
Hpy166II GTNNAC 1 cut(s) 934
Hpy188I TCNGA 5 cut(s) 220, 273, 346, 624, 687
Hpy188III TCNNGA 2 cut(s) 279, 914
Hpy8I GTNNAC 1 cut(s) 934
Hpy99I CGWCG 1 cut(s) 38
HpyAV CCTTC 7 cut(s) 129, 133, 153, 596, 896, 977, 992
HpyCH4III ACNGT 1 cut(s) 1006
HpyCH4V TGCA 4 cut(s) 4, 108, 160, 571
HpyF10VI GCNNNNNNNGC 3 cut(s) 46, 78, 882
HpyF3I CTNAG 3 cut(s) 398, 561, 954
Hsp92II CATG 1 cut(s) 461
HspAI GCGC 1 cut(s) 846
Kzo9I GATC 9 cut(s) 12, 94, 268, 337, 418, 586, 720, 816, 838
LmnI GCTCC 4 cut(s) 45, 77, 895, 916
Lsp1109I GCAGC 1 cut(s) 984
LweI GCATC 1 cut(s) 90
MaeI CTAG 2 cut(s) 134, 353
MaeIII GTNAC 2 cut(s) 212, 1006
MalI GATC 9 cut(s) 14, 96, 270, 339, 420, 588, 722, 818, 840
MbiI CCGCTC 1 cut(s) 72
MboI GATC 9 cut(s) 12, 94, 268, 337, 418, 586, 720, 816, 838
MboII GAAGA 6 cut(s) 94, 278, 416, 666, 811, 919
MflI RGATCY 1 cut(s) 268
MhlI GDGCHC 3 cut(s) 42, 195, 900
MlsI TGGCCA 1 cut(s) 515
MluNI TGGCCA 1 cut(s) 515
MmeI TCCRAC 2 cut(s) 339, 1012
Mox20I TGGCCA 1 cut(s) 515
MscI TGGCCA 1 cut(s) 515
MseI TTAA 1 cut(s) 386
Msp20I TGGCCA 1 cut(s) 515
MspI CCGG 2 cut(s) 90, 893
MspR9I CCNGG 2 cut(s) 302, 927
MvaI CCWGG 2 cut(s) 302, 927
MvnI CGCG 1 cut(s) 33
MwoI GCNNNNNNNGC 3 cut(s) 46, 78, 882
NdeII GATC 9 cut(s) 12, 94, 268, 337, 418, 586, 720, 816, 838
NlaIII CATG 1 cut(s) 461
NlaIV GGNNCC 3 cut(s) 316, 375, 918
NmeAIII GCCGAG 1 cut(s) 57
NmuCI GTSAC 1 cut(s) 1006
NsbI TGCGCA 1 cut(s) 847
NspI RCATGY 1 cut(s) 461
NspV TTCGAA 3 cut(s) 203, 287, 293
PciI ACATGT 1 cut(s) 457
PcsI WCGNNNNNNNCGW 1 cut(s) 489
PfeI GAWTC 5 cut(s) 173, 254, 494, 725, 910
PflMI CCANNNNNTGG 1 cut(s) 926
PkrI GCNGC 1 cut(s) 974
PscI ACATGT 1 cut(s) 457
Psp124BI GAGCTC 2 cut(s) 42, 900
Psp6I CCWGG 2 cut(s) 300, 925
PspGI CCWGG 2 cut(s) 300, 925
PspN4I GGNNCC 3 cut(s) 316, 375, 918
PsuI RGATCY 1 cut(s) 268
RsaI GTAC 3 cut(s) 368, 396, 456
RsaNI GTAC 3 cut(s) 367, 395, 455
SacI GAGCTC 2 cut(s) 42, 900
SalI GTCGAC 1 cut(s) 932
SaqAI TTAA 1 cut(s) 386
SatI GCNGC 1 cut(s) 973
Sau3AI GATC 9 cut(s) 12, 94, 268, 337, 418, 586, 720, 816, 838
ScrFI CCNGG 2 cut(s) 302, 927
SduI GDGCHC 3 cut(s) 42, 195, 900
SfaNI GCATC 1 cut(s) 90
SfuI TTCGAA 3 cut(s) 203, 287, 293
SsiI CCGC 6 cut(s) 31, 70, 168, 391, 854, 864
SspMI CTAG 2 cut(s) 134, 353
SstI GAGCTC 2 cut(s) 42, 900
StyD4I CCNGG 2 cut(s) 300, 925
TaaI ACNGT 1 cut(s) 1006
TatI WGTACW 1 cut(s) 454
TfiI GAWTC 5 cut(s) 173, 254, 494, 725, 910
Tru1I TTAA 1 cut(s) 386
Tru9I TTAA 1 cut(s) 386
TscAI CASTG 3 cut(s) 589, 927, 1011
TseFI GTSAC 1 cut(s) 1006
TseI GCWGC 1 cut(s) 972
Tsp45I GTSAC 1 cut(s) 1006
TspDTI ATGAA 2 cut(s) 537, 920
TspGWI ACGGA 2 cut(s) 200, 330
TspRI CASTG 3 cut(s) 589, 927, 1011
Van91I CCANNNNNTGG 1 cut(s) 926
XapI RAATTY 4 cut(s) 154, 205, 289, 625
XceI RCATGY 1 cut(s) 461
XmiI GTMKAC 1 cut(s) 933
XspI CTAG 2 cut(s) 134, 353
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.