pycom10g09590

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0000910
Physical Location & Seq
Forward (+)
12712536 .. 12714435
1900 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g09590.5

Sequence Viewer

Length: 1635 bp
ATGAGTATAAAGATAACTTTATATTTTGAATTGGGTTTGAGAAGGTGTAAAAAGAAATTATATGTAATAATGGAGGTGGATTGTCTGCCCACCCATTTTCATAGCAATAATAAAACAAAAAGTCATAAGAATATAAAATCCACCTCCAAAAATAATACCTATGCTTTAAGAGTAAAAAGAAAAATGGGACTTGGGGAGTGCGATCGACAGAAACCAAAGAAACCATTCGAGTGGAGGTCCCAGATCGATCTCTGCTCTCTCCACCACCTCAAACAAACCGTCTCCCAACTAATCTCCGCCGCTCCTTCCTCCTTCTGCCTCTCCCTCAACCACCGCGACGAGCTCCAAGCGCCATCCCCTCACGATTCCCTCCGCTCCCTCGGCATCACCTCCGGCGATCTTGTCTTCTACACCCTCGACCCCTCCTCCCAAACCCTAGCTTTGCCTTCCCATCCAAATTTGCACTCCTCCGCAGAATCCCCAAATCGAATTGAGCCCCAATGTTCGCATTTCCGTAACTCCGAAACCCTAATGGACAAAGGGGGTGTTATGGACGATTCGATTGGTGAAGATCCGAGCTCTCGAGGTTCGAATTCGGAAACCCTGGTAGTTTCTGGTGCCCAAAGTATGGAAATTGATGATCCGTCTGATGGGCTAGGGTTGGAAAGGTACTCGGTTCCATTTTTCTTGAAGCGGGTACTGAGGGAGGAGTTGGGAGAGGATCGTAGCAATCATCATAAGCTATTGTTGATTTTTGTACATGCTGTGCTATTAGAGTCTGGTTTCGTTGGGTTCGATTCACTTTCGAGTATGTTGGCCAATCAAAGGACGGGTTCAACGATTTCGCTTTCATATACTTTGCCTGAGCTTTTGCGAAATCGGGGCAGTGATCGTAATGGGGTTGAAGGGGTTGTGTTTAAGTTTCAGAATTTGGGGCATTTTGTGAATGTTTATGGTTCTTTGGCCTTTGGCGGTTCTTCACTCCATCAGATATGTTTGGATAAAAACAGATTTGCCCCAATGATCGAATCCATTTGGGAAATCCAAAATGTGCATGAGAGAGATGGGTTAGCCCCAGAGAGAGAAGTTTTAGAGTTTTGGAAGATTGTGAAAGATGGGATCACATTTCCATTGCTGATTGATCTTTGCGCAATGGCGGGTTTGCCCGCCCCACCAAGCCTTATGCGCCTTCCACCGGAGCTCAAAATGAAGATTCTGGAGCCACTGCCTGGTGTCGACATTGCCAAGGTGGGTTGTGTTTGTAAGGAGCTGCAAAATCATGCTAATAATGATGAATTGTGGAAGCAGAAGTATGCTGAGGAGTTCGGTAATGGCAGCAGAGGAGAAGGAACTGGAGGTGAAGGAACAGTGACTAACTGGAAGTTTAGGTTTGTTGGAAATTGGGAGACTAGGAATGAGACGCCTTATTTCAACCATATCAGGAGGGACCATAACCCACTTGTACCTCCAAGGATTGGTGGAATAATTGGTGGGGATTATGACCGCTTTCCACTCCTAGGTGCCGTCGATCCTACTGGACAAGCACATCCTCTACAACAACCCCGAATATCTTATATGCGGCGTAATTTTTCACCCAACTGCAATCTGGGCAGTCTGGGAGGGTTCAATATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like HLH

Protein Analysis

545

Amino Acids

60.85

Weight (kDa)

7.64

Isoelectric Point (pI)

50.7

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1150
AccB1I GGYRCC 2 cut(s) 617, 1520
AccB7I CCANNNNNTGG 3 cut(s) 628, 1229, 1475
AccBSI CCGCTC 2 cut(s) 302, 375
AccI GTMKAC 1 cut(s) 1236
AccII CGCG 1 cut(s) 336
AclWI GGATC 5 cut(s) 566, 635, 729, 1127, 1523
AcoI YGGCCR 1 cut(s) 816
AcsI RAATTY 3 cut(s) 457, 592, 928
AcyI GRCGYC 1 cut(s) 1421
AfaI GTAC 4 cut(s) 671, 699, 759, 1464
AfiI CCNNNNNNNGG 7 cut(s) 628, 650, 825, 1195, 1229, 1475, 1517
AgsI TTSAA 6 cut(s) 29, 691, 837, 905, 1432, 1627
AjnI CCWGG 2 cut(s) 603, 1228
AluBI AGCT 7 cut(s) 343, 440, 579, 742, 868, 1201, 1270
AluI AGCT 7 cut(s) 343, 440, 579, 742, 868, 1201, 1270
Alw21I GWGCWC 3 cut(s) 345, 581, 1203
Alw26I GTCTC 3 cut(s) 286, 1400, 1412
AlwI GGATC 5 cut(s) 566, 635, 729, 1127, 1523
Ama87I CYCGRG 1 cut(s) 582
AoxI GGCC 2 cut(s) 816, 963
ApeKI GCWGC 2 cut(s) 1270, 1335
ApoI RAATTY 3 cut(s) 457, 592, 928
ArsI GACNNNNNNTTYG 2 cut(s) 264, 296
Asp700I GAANNNNTTC 1 cut(s) 224
AspA2I CCTAGG 1 cut(s) 1516
AspLEI GCGC 3 cut(s) 352, 1151, 1188
AspS9I GGNCC 2 cut(s) 237, 1447
AsuHPI GGTGA 4 cut(s) 379, 578, 1370, 1584
AsuII TTCGAA 1 cut(s) 590
AvaI CYCGRG 1 cut(s) 582
AvaII GGWCC 2 cut(s) 237, 1447
AvrII CCTAGG 1 cut(s) 1516
BaeGI GKGCMC 1 cut(s) 622
BalI TGGCCA 1 cut(s) 818
BanI GGYRCC 2 cut(s) 617, 1520
BanII GRGCYC 4 cut(s) 345, 498, 581, 1203
BbsI GAAGAC 1 cut(s) 397
Bbv12I GWGCWC 3 cut(s) 345, 581, 1203
BbvCI CCTCAGC 1 cut(s) 1317
BbvI GCAGC 2 cut(s) 1257, 1347
BccI CCATC 6 cut(s) 361, 459, 644, 993, 1058, 1109
BceAI ACGGC 1 cut(s) 1508
BciT130I CCWGG 2 cut(s) 605, 1230
BcoDI GTCTC 3 cut(s) 286, 1400, 1412
BfaI CTAG 4 cut(s) 437, 656, 1410, 1517
BfoI RGCGCY 1 cut(s) 353
BisI GCNGC 4 cut(s) 300, 1271, 1336, 1580
BlnI CCTAGG 1 cut(s) 1516
BlsI GCNGC 4 cut(s) 301, 1272, 1337, 1581
Bme1390I CCNGG 2 cut(s) 605, 1230
Bme18I GGWCC 2 cut(s) 237, 1447
BmeT110I CYCGRG 1 cut(s) 582
BmgT120I GGNCC 2 cut(s) 237, 1447
BmiI GGNNCC 6 cut(s) 239, 619, 678, 1221, 1448, 1522
BmrFI CCNGG 2 cut(s) 605, 1230
BmsI GCATC 1 cut(s) 393
BpiI GAAGAC 1 cut(s) 397
BpmI CTGGAG 2 cut(s) 1238, 1374
Bpu10I CCTNAGC 2 cut(s) 864, 1317
Bpu14I TTCGAA 1 cut(s) 590
Bsa29I ATCGAT 1 cut(s) 246
BsaHI GRCGYC 1 cut(s) 1421
BsaJI CCNNGG 5 cut(s) 379, 603, 1245, 1469, 1516
BsaWI WCCGGW 1 cut(s) 1195
BsaXI ACNNNNNCTCC 2 cut(s) 410, 440
Bsc4I CCNNNNNNNGG 7 cut(s) 628, 650, 825, 1195, 1229, 1475, 1517
Bse1I ACTGG 3 cut(s) 1357, 1382, 1540
Bse3DI GCAATG 3 cut(s) 1130, 1158, 1239
BseBI CCWGG 2 cut(s) 605, 1230
BseCI ATCGAT 1 cut(s) 246
BseDI CCNNGG 5 cut(s) 379, 603, 1245, 1469, 1516
BseGI GGATG 3 cut(s) 353, 451, 1546
BseLI CCNNNNNNNGG 7 cut(s) 628, 650, 825, 1195, 1229, 1475, 1517
BseMI GCAATG 3 cut(s) 1130, 1158, 1239
BseMII CTCAG 3 cut(s) 692, 855, 1308
BseNI ACTGG 3 cut(s) 1357, 1382, 1540
BseRI GAGGAG 5 cut(s) 415, 457, 722, 1334, 1356
BseSI GKGCMC 1 cut(s) 622
BseXI GCAGC 2 cut(s) 1257, 1347
Bsh1236I CGCG 1 cut(s) 336
Bsh1285I CGRYCG 1 cut(s) 205
BshFI GGCC 2 cut(s) 818, 965
BshNI GGYRCC 2 cut(s) 617, 1520
BshVI ATCGAT 1 cut(s) 246
BsiEI CGRYCG 1 cut(s) 205
BsiHKAI GWGCWC 3 cut(s) 345, 581, 1203
BsiHKCI CYCGRG 1 cut(s) 582
BsiSI CCGG 2 cut(s) 393, 1196
BslFI GGGAC 3 cut(s) 201, 223, 1460
BslI CCNNNNNNNGG 7 cut(s) 628, 650, 825, 1195, 1229, 1475, 1517
BsmAI GTCTC 3 cut(s) 286, 1400, 1412
BsmBI CGTCTC 2 cut(s) 286, 1412
BsmFI GGGAC 3 cut(s) 201, 223, 1460
BsnI GGCC 2 cut(s) 818, 965
BsoBI CYCGRG 1 cut(s) 582
Bsp119I TTCGAA 1 cut(s) 590
Bsp1286I GDGCHC 5 cut(s) 345, 498, 581, 622, 1203
Bsp1407I TGTACA 1 cut(s) 757
BspANI GGCC 2 cut(s) 818, 965
BspCNI CTCAG 3 cut(s) 693, 856, 1309
BspDI ATCGAT 1 cut(s) 246
BspFNI CGCG 1 cut(s) 336
BspLI GGNNCC 6 cut(s) 239, 619, 678, 1221, 1448, 1522
BspPI GGATC 5 cut(s) 566, 635, 729, 1127, 1523
BspT104I TTCGAA 1 cut(s) 590
BspT107I GGYRCC 2 cut(s) 617, 1520
BsrBI CCGCTC 2 cut(s) 302, 375
BsrDI GCAATG 3 cut(s) 1130, 1158, 1239
BsrGI TGTACA 1 cut(s) 757
BsrI ACTGG 3 cut(s) 1357, 1382, 1540
BssECI CCNNGG 5 cut(s) 379, 603, 1245, 1469, 1516
BssNI GRCGYC 1 cut(s) 1421
BssT1I CCWWGG 3 cut(s) 1245, 1469, 1516
Bst2UI CCWGG 2 cut(s) 605, 1230
Bst4CI ACNGT 2 cut(s) 280, 1369
BstACI GRCGYC 1 cut(s) 1421
BstAUI TGTACA 1 cut(s) 757
BstBI TTCGAA 1 cut(s) 590
BstC8I GCNNGC 1 cut(s) 1167
BstDEI CTNAG 3 cut(s) 701, 864, 1317
BstF5I GGATG 3 cut(s) 353, 451, 1546
BstFNI CGCG 1 cut(s) 336
BstH2I RGCGCY 1 cut(s) 353
BstHHI GCGC 3 cut(s) 352, 1151, 1188
BstMAI GTCTC 3 cut(s) 286, 1400, 1412
BstMCI CGRYCG 1 cut(s) 205
BstMWI GCNNNNNNNGC 4 cut(s) 349, 381, 1185, 1608
BstNI CCWGG 2 cut(s) 605, 1230
BstNSI RCATGY 1 cut(s) 764
BstSCI CCNGG 2 cut(s) 603, 1228
BstSLI GKGCMC 1 cut(s) 622
BstUI CGCG 1 cut(s) 336
BstV1I GCAGC 2 cut(s) 1257, 1347
BstV2I GAAGAC 1 cut(s) 397
BstX2I RGATCY 1 cut(s) 571
BstXI CCANNNNNNTGG 1 cut(s) 231
BstYI RGATCY 1 cut(s) 571
Bsu15I ATCGAT 1 cut(s) 246
BsuRI GGCC 2 cut(s) 818, 965
BsuTUI ATCGAT 1 cut(s) 246
BtsCI GGATG 3 cut(s) 353, 451, 1546
BtsI GCAGTG 2 cut(s) 892, 1223
BtsIMutI CAGTG 3 cut(s) 892, 1223, 1374
Cac8I GCNNGC 1 cut(s) 1167
CfoI GCGC 3 cut(s) 352, 1151, 1188
Cfr13I GGNCC 2 cut(s) 237, 1447
ClaI ATCGAT 1 cut(s) 246
CseI GACGC 1 cut(s) 1429
Csp6I GTAC 4 cut(s) 670, 698, 758, 1463
CviAII CATG 3 cut(s) 761, 1055, 1280
CviQI GTAC 4 cut(s) 670, 698, 758, 1463
DdeI CTNAG 3 cut(s) 701, 864, 1317
EaeI YGGCCR 1 cut(s) 816
EciI GGCGGA 1 cut(s) 286
Ecl136II GAGCTC 3 cut(s) 343, 579, 1201
Eco130I CCWWGG 3 cut(s) 1245, 1469, 1516
Eco24I GRGCYC 4 cut(s) 345, 498, 581, 1203
Eco47I GGWCC 2 cut(s) 237, 1447
Eco53kI GAGCTC 3 cut(s) 343, 579, 1201
Eco88I CYCGRG 1 cut(s) 582
EcoICRI GAGCTC 3 cut(s) 343, 579, 1201
EcoO109I RGGNCCY 1 cut(s) 237
EcoRI GAATTC 1 cut(s) 592
EcoRII CCWGG 2 cut(s) 603, 1228
EcoT14I CCWWGG 3 cut(s) 1245, 1469, 1516
EcoT38I GRGCYC 4 cut(s) 345, 498, 581, 1203
ErhI CCWWGG 3 cut(s) 1245, 1469, 1516
Esp3I CGTCTC 2 cut(s) 286, 1412
FaeI CATG 3 cut(s) 764, 1058, 1283
FaqI GGGAC 3 cut(s) 201, 223, 1460
FatI CATG 3 cut(s) 760, 1054, 1279
FauI CCCGC 3 cut(s) 687, 1150, 1174
FblI GTMKAC 1 cut(s) 1236
Fnu4HI GCNGC 4 cut(s) 300, 1271, 1336, 1580
FokI GGATG 3 cut(s) 340, 438, 1533
FriOI GRGCYC 4 cut(s) 345, 498, 581, 1203
Fsp4HI GCNGC 4 cut(s) 300, 1271, 1336, 1580
FspBI CTAG 4 cut(s) 437, 656, 1410, 1517
FspI TGCGCA 1 cut(s) 1150
GlaI GCGC 3 cut(s) 351, 1150, 1187
GluI GCNGC 4 cut(s) 300, 1271, 1336, 1580
GsuI CTGGAG 2 cut(s) 1238, 1374
HaeII RGCGCY 1 cut(s) 353
HaeIII GGCC 2 cut(s) 818, 965
HapII CCGG 2 cut(s) 393, 1196
HgaI GACGC 1 cut(s) 1429
HhaI GCGC 3 cut(s) 352, 1151, 1188
Hin1I GRCGYC 1 cut(s) 1421
Hin1II CATG 3 cut(s) 764, 1058, 1283
Hin6I GCGC 3 cut(s) 350, 1149, 1186
HinP1I GCGC 3 cut(s) 350, 1149, 1186
HincII GTYRAC 1 cut(s) 1237
HindII GTYRAC 1 cut(s) 1237
HinfI GANTC 7 cut(s) 365, 476, 557, 776, 797, 1028, 1213
HpaII CCGG 2 cut(s) 393, 1196
HphI GGTGA 4 cut(s) 379, 578, 1370, 1584
Hpy166II GTNNAC 1 cut(s) 1237
Hpy188I TCNGA 6 cut(s) 523, 576, 598, 649, 927, 990
Hpy188III TCNNGA 5 cut(s) 362, 582, 688, 1217, 1441
Hpy8I GTNNAC 1 cut(s) 1237
Hpy99I CGWCG 2 cut(s) 341, 1529
HpyAV CCTTC 8 cut(s) 36, 315, 322, 456, 899, 1199, 1340, 1355
HpyCH4III ACNGT 2 cut(s) 280, 1369
HpyCH4V TGCA 4 cut(s) 463, 1054, 1273, 1602
HpyF10VI GCNNNNNNNGC 4 cut(s) 349, 381, 1185, 1608
HpyF3I CTNAG 3 cut(s) 701, 864, 1317
Hsp92I GRCGYC 1 cut(s) 1421
Hsp92II CATG 3 cut(s) 764, 1058, 1283
HspAI GCGC 3 cut(s) 350, 1149, 1186
LmnI GCTCC 6 cut(s) 307, 348, 380, 1198, 1219, 1267
Lsp1109I GCAGC 2 cut(s) 1257, 1347
LweI GCATC 1 cut(s) 393
MaeI CTAG 4 cut(s) 437, 656, 1410, 1517
MaeIII GTNAC 2 cut(s) 515, 1369
MbiI CCGCTC 2 cut(s) 302, 375
MboII GAAGA 5 cut(s) 397, 581, 969, 1114, 1222
MflI RGATCY 1 cut(s) 571
MhlI GDGCHC 5 cut(s) 345, 498, 581, 622, 1203
MlsI TGGCCA 1 cut(s) 818
MluNI TGGCCA 1 cut(s) 818
MlyI GAGTC 1 cut(s) 785
MmeI TCCRAC 2 cut(s) 642, 1375
Mox20I TGGCCA 1 cut(s) 818
MroXI GAANNNNTTC 1 cut(s) 224
MscI TGGCCA 1 cut(s) 818
MseI TTAA 2 cut(s) 167, 918
MslI CAYNNNNRTG 1 cut(s) 229
Msp20I TGGCCA 1 cut(s) 818
MspI CCGG 2 cut(s) 393, 1196
MspR9I CCNGG 2 cut(s) 605, 1230
MvaI CCWGG 2 cut(s) 605, 1230
MvnI CGCG 1 cut(s) 336
MwoI GCNNNNNNNGC 4 cut(s) 349, 381, 1185, 1608
NlaIII CATG 3 cut(s) 764, 1058, 1283
NlaIV GGNNCC 6 cut(s) 239, 619, 678, 1221, 1448, 1522
NmeAIII GCCGAG 1 cut(s) 360
NmuCI GTSAC 1 cut(s) 1369
NsbI TGCGCA 1 cut(s) 1150
NspI RCATGY 1 cut(s) 764
NspV TTCGAA 1 cut(s) 590
PaeR7I CTCGAG 1 cut(s) 582
PcsI WCGNNNNNNNCGW 2 cut(s) 792, 836
PdmI GAANNNNTTC 1 cut(s) 224
PfeI GAWTC 6 cut(s) 365, 476, 557, 797, 1028, 1213
PflMI CCANNNNNTGG 3 cut(s) 628, 1229, 1475
PkrI GCNGC 4 cut(s) 301, 1272, 1337, 1581
Ple19I CGATCG 1 cut(s) 205
PleI GAGTC 1 cut(s) 784
PpsI GAGTC 1 cut(s) 784
PpuMI RGGWCCY 1 cut(s) 237
Psp124BI GAGCTC 3 cut(s) 345, 581, 1203
Psp5II RGGWCCY 1 cut(s) 237
Psp6I CCWGG 2 cut(s) 603, 1228
PspGI CCWGG 2 cut(s) 603, 1228
PspN4I GGNNCC 6 cut(s) 239, 619, 678, 1221, 1448, 1522
PspPI GGNCC 2 cut(s) 237, 1447
PspPPI RGGWCCY 1 cut(s) 237
PsuI RGATCY 1 cut(s) 571
PvuI CGATCG 1 cut(s) 205
RsaI GTAC 4 cut(s) 671, 699, 759, 1464
RsaNI GTAC 4 cut(s) 670, 698, 758, 1463
RseI CAYNNNNRTG 1 cut(s) 229
SacI GAGCTC 3 cut(s) 345, 581, 1203
SalI GTCGAC 1 cut(s) 1235
SaqAI TTAA 2 cut(s) 167, 918
SatI GCNGC 4 cut(s) 300, 1271, 1336, 1580
Sau96I GGNCC 2 cut(s) 237, 1447
SchI GAGTC 1 cut(s) 785
ScrFI CCNGG 2 cut(s) 605, 1230
SduI GDGCHC 5 cut(s) 345, 498, 581, 622, 1203
SfaNI GCATC 1 cut(s) 393
Sfr274I CTCGAG 1 cut(s) 582
SfuI TTCGAA 1 cut(s) 590
SinI GGWCC 2 cut(s) 237, 1447
SlaI CTCGAG 1 cut(s) 582
SmiMI CAYNNNNRTG 1 cut(s) 229
SmlI CTYRAG 1 cut(s) 582
SmoI CTYRAG 1 cut(s) 582
SspMI CTAG 4 cut(s) 437, 656, 1410, 1517
SstI GAGCTC 3 cut(s) 345, 581, 1203
StyD4I CCNGG 2 cut(s) 603, 1228
StyI CCWWGG 3 cut(s) 1245, 1469, 1516
TaaI ACNGT 2 cut(s) 280, 1369
TatI WGTACW 1 cut(s) 757
TauI GCSGC 2 cut(s) 302, 1582
TfiI GAWTC 6 cut(s) 365, 476, 557, 797, 1028, 1213
Tru1I TTAA 2 cut(s) 167, 918
Tru9I TTAA 2 cut(s) 167, 918
TscAI CASTG 3 cut(s) 892, 1230, 1374
TseFI GTSAC 1 cut(s) 1369
TseI GCWGC 2 cut(s) 1270, 1335
Tsp45I GTSAC 1 cut(s) 1369
TspDTI ATGAA 4 cut(s) 89, 840, 1223, 1308
TspGWI ACGGA 2 cut(s) 503, 633
TspRI CASTG 3 cut(s) 892, 1230, 1374
Van91I CCANNNNNTGG 3 cut(s) 628, 1229, 1475
VpaK11BI GGWCC 2 cut(s) 237, 1447
XapI RAATTY 3 cut(s) 457, 592, 928
XceI RCATGY 1 cut(s) 764
XcmI CCANNNNNNNNNTGG 1 cut(s) 1603
XhoI CTCGAG 1 cut(s) 582
XmaJI CCTAGG 1 cut(s) 1516
XmiI GTMKAC 1 cut(s) 1236
XmnI GAANNNNTTC 1 cut(s) 224
XspI CTAG 4 cut(s) 437, 656, 1410, 1517
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.