Rh4BG142000

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0000910
Physical Location & Seq
Reverse (-)
25192600 .. 25193759
1160 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG142000.1

Sequence Viewer

Length: 1047 bp
ATGGCGTTTGAACTAGGACAGCGGACACCAATGGGTGAAGAAGACGAAGAGAGGGCTTGTGATCCTTTGAAAGACGACAAGTACAAGTACTCCCCATCTTTATTGTTGAGGAGGGTACTCACAGAGGAGTTGGCCGCCCAAGACCTTCACAGAAATCACAAAAACAACAAGCTTCTCGTCACTGCGGTGCATGCTGTGCTCTTAGACTCCGGTTTCGTTCGCTTTAGTTCGGGTACGTCAGTACATGATGCCCTATTAGAGTTAGGTTTCACCGAGTTCGGTTCGGTATCGGGTATGTCACATTCCTACACTTTGCCTGAGATTCTTATCGAACAAGATAATTGCGATTGTAGTAGTAATCAGAAGATGGAAGGAATTGTTTTGAAGTTTGAGAATTCAGGTAACTTTTCGTCTGACCAGAAAAGAACAAAACATGTGGCTCCTCGCATGGTGAAAGTTAGTGGGTCCTTGATTAATTCTGGTTCTAGGTTTGGGCAGAAGCACAAGAAGGTATGTTTGGAATTGGATGAGAATAAGTTTGCGCCGGCGATTGAGTTTTTTTGGGGAAATAATACGAGGAATACTGTGAATGATTTGCGATTTCGTGAGAGACAAGTTTGTGAGTTTTGGAAAATTGTCAAGGATGAGATTGCACTTCCATTGCTGATTGATCTTTGCGCAGAGGCCGGTTGGCCTGCTCCATTGTGCCTTATGAGCCTTCCTCCGGAGCTCAAAATGAAGATTTTGGAGGCAATGCCAGGTGCTGATCTTGCCAGAGTAGGGGGTGTTTGTAAGGAGCTACGAGATCTTGCAAATGACAATGAGTTGTGGAAGCACAACTTAGCCCAGGAGTTTGCTGAGGAGTTTTCTGGTACAAGAGAAGTAGAAGAACTCTGGAAAGTTACTTTTCGTCGACAATGGGAGACTAAAAGGGAGCAAGCAAAGAAACTAGTGGGAAAAAAGAGAGGACGTGAGAACGTTTATTGTTTCCATTGGCAACCACGTCCTAAATATCCTAAATGGAGTCATGAACCATATTTCTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like HLH UN_NPL4

Protein Analysis

348

Amino Acids

40.04

Weight (kDa)

6.81

Isoelectric Point (pI)

51.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 238 - 279 9.4e-06 F-box domain
F-box-like PF12937 239 - 279 3.3e-10 F-box-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 679
AccI GTMKAC 1 cut(s) 913
AccIII TCCGGA 1 cut(s) 724
AciI CCGC 3 cut(s) 22, 135, 185
AclI AACGTT 1 cut(s) 978
AclWI GGATC 1 cut(s) 56
AcoI YGGCCR 1 cut(s) 132
AcsI RAATTY 1 cut(s) 394
AfaI GTAC 6 cut(s) 83, 89, 117, 235, 243, 874
AfiI CCNNNNNNNGG 2 cut(s) 724, 780
AflIII ACRYGT 1 cut(s) 433
AgsI TTSAA 3 cut(s) 11, 70, 385
AhlI ACTAGT 1 cut(s) 949
AjiI CACGTC 2 cut(s) 971, 1004
AjnI CCWGG 2 cut(s) 757, 846
AjuI GAANNNNNNNTTGG 2 cut(s) 500, 532
AleI CACNNNNGTG 1 cut(s) 185
AluBI AGCT 3 cut(s) 172, 730, 799
AluI AGCT 3 cut(s) 172, 730, 799
Alw21I GWGCWC 2 cut(s) 201, 732
Alw26I GTCTC 2 cut(s) 604, 917
AlwI GGATC 1 cut(s) 56
AlwNI CAGNNNCTG 1 cut(s) 764
Aor13HI TCCGGA 1 cut(s) 724
AoxI GGCC 3 cut(s) 132, 684, 692
ApoI RAATTY 1 cut(s) 394
ArsI GACNNNNNNTTYG 2 cut(s) 197, 229
AseI ATTAAT 1 cut(s) 474
AspLEI GCGC 2 cut(s) 544, 680
AspS9I GGNCC 1 cut(s) 465
AsuHPI GGTGA 3 cut(s) 47, 262, 463
AvaII GGWCC 1 cut(s) 465
BaeI ACNNNNGTAYC 2 cut(s) 225, 258
BanII GRGCYC 1 cut(s) 732
BbsI GAAGAC 1 cut(s) 48
Bbv12I GWGCWC 2 cut(s) 201, 732
BbvCI CCTCAGC 1 cut(s) 858
BccI CCATC 2 cut(s) 103, 361
BciT130I CCWGG 2 cut(s) 759, 848
BcoDI GTCTC 2 cut(s) 604, 917
BcuI ACTAGT 1 cut(s) 949
BfaI CTAG 3 cut(s) 14, 486, 950
BglII AGATCT 1 cut(s) 805
BisI GCNGC 1 cut(s) 135
BlsI GCNGC 1 cut(s) 136
BmcAI AGTACT 1 cut(s) 89
Bme1390I CCNGG 2 cut(s) 759, 848
Bme18I GGWCC 1 cut(s) 465
BmgBI CACGTC 2 cut(s) 971, 1004
BmgT120I GGNCC 1 cut(s) 465
BmiI GGNNCC 2 cut(s) 441, 466
BmrFI CCNGG 2 cut(s) 759, 848
BmsI GCATC 1 cut(s) 238
BpiI GAAGAC 1 cut(s) 48
BplI GAGNNNNNCTC 2 cut(s) 706, 738
Bpu10I CCTNAGC 1 cut(s) 858
BsaBI GATNNNNATC 1 cut(s) 326
BsaJI CCNNGG 1 cut(s) 846
BsaWI WCCGGW 2 cut(s) 209, 724
BsaXI ACNNNNNCTCC 2 cut(s) 74, 104
Bsc4I CCNNNNNNNGG 2 cut(s) 724, 780
Bse118I RCCGGY 2 cut(s) 544, 686
Bse3DI GCAATG 2 cut(s) 659, 759
Bse8I GATNNNNATC 1 cut(s) 326
BseAI TCCGGA 1 cut(s) 724
BseBI CCWGG 2 cut(s) 759, 848
BseDI CCNNGG 1 cut(s) 846
BseGI GGATG 2 cut(s) 532, 649
BseJI GATNNNNATC 1 cut(s) 326
BseLI CCNNNNNNNGG 2 cut(s) 724, 780
BseMI GCAATG 2 cut(s) 659, 759
BseMII CTCAG 2 cut(s) 309, 849
BseRI GAGGAG 4 cut(s) 124, 140, 432, 875
BshFI GGCC 3 cut(s) 134, 686, 694
BsiHKAI GWGCWC 2 cut(s) 201, 732
BsiSI CCGG 4 cut(s) 210, 545, 687, 725
BslI CCNNNNNNNGG 2 cut(s) 724, 780
BsmAI GTCTC 2 cut(s) 604, 917
BsnI GGCC 3 cut(s) 134, 686, 694
Bsp1286I GDGCHC 2 cut(s) 201, 732
Bsp13I TCCGGA 1 cut(s) 724
Bsp143I GATC 4 cut(s) 61, 670, 766, 805
BspACI CCGC 3 cut(s) 22, 135, 185
BspANI GGCC 3 cut(s) 134, 686, 694
BspCNI CTCAG 2 cut(s) 310, 850
BspEI TCCGGA 1 cut(s) 724
BspHI TCATGA 1 cut(s) 1027
BspLI GGNNCC 2 cut(s) 441, 466
BspPI GGATC 1 cut(s) 56
BsrDI GCAATG 2 cut(s) 659, 759
BsrFI RCCGGY 2 cut(s) 544, 686
BssAI RCCGGY 2 cut(s) 544, 686
BssECI CCNNGG 1 cut(s) 846
BssMI GATC 4 cut(s) 61, 670, 766, 805
Bst2UI CCWGG 2 cut(s) 759, 848
Bst4CI ACNGT 1 cut(s) 586
Bst6I CTCTTC 1 cut(s) 42
BstAPI GCANNNNNTGC 1 cut(s) 196
BstC8I GCNNGC 4 cut(s) 192, 546, 696, 939
BstDEI CTNAG 4 cut(s) 202, 318, 841, 858
BstF5I GGATG 2 cut(s) 532, 649
BstHHI GCGC 2 cut(s) 544, 680
BstKTI GATC 4 cut(s) 64, 673, 769, 808
BstMAI GTCTC 2 cut(s) 604, 917
BstMBI GATC 4 cut(s) 61, 670, 766, 805
BstMWI GCNNNNNNNGC 4 cut(s) 191, 196, 714, 770
BstNI CCWGG 2 cut(s) 759, 848
BstNSI RCATGY 2 cut(s) 194, 437
BstSCI CCNGG 2 cut(s) 757, 846
BstV2I GAAGAC 1 cut(s) 48
BstX2I RGATCY 1 cut(s) 805
BstYI RGATCY 1 cut(s) 805
BsuRI GGCC 3 cut(s) 134, 686, 694
BtrI CACGTC 2 cut(s) 971, 1004
BtsCI GGATG 2 cut(s) 532, 649
BtsI GCAGTG 1 cut(s) 180
BtsIMutI CAGTG 1 cut(s) 180
Cac8I GCNNGC 4 cut(s) 192, 546, 696, 939
CaiI CAGNNNCTG 1 cut(s) 764
CciI TCATGA 1 cut(s) 1027
CfoI GCGC 2 cut(s) 544, 680
Cfr10I RCCGGY 2 cut(s) 544, 686
Cfr13I GGNCC 1 cut(s) 465
Csp6I GTAC 6 cut(s) 82, 88, 116, 234, 242, 873
CspCI CAANNNNNGTGG 2 cut(s) 417, 452
CviAII CATG 5 cut(s) 191, 245, 434, 448, 1028
CviQI GTAC 6 cut(s) 82, 88, 116, 234, 242, 873
DdeI CTNAG 4 cut(s) 202, 318, 841, 858
DpnI GATC 4 cut(s) 63, 672, 768, 807
DpnII GATC 4 cut(s) 61, 670, 766, 805
EaeI YGGCCR 1 cut(s) 132
Eam1104I CTCTTC 1 cut(s) 42
EarI CTCTTC 1 cut(s) 42
Ecl136II GAGCTC 1 cut(s) 730
Eco24I GRGCYC 1 cut(s) 732
Eco47I GGWCC 1 cut(s) 465
Eco53kI GAGCTC 1 cut(s) 730
EcoICRI GAGCTC 1 cut(s) 730
EcoO109I RGGNCCY 1 cut(s) 465
EcoRI GAATTC 1 cut(s) 394
EcoRII CCWGG 2 cut(s) 757, 846
EcoT38I GRGCYC 1 cut(s) 732
FaeI CATG 5 cut(s) 194, 248, 437, 451, 1031
FaiI YATR 9 cut(s) 192, 246, 296, 435, 449, 514, 713, 1029, 1036
FalI AAGNNNNNCTT 2 cut(s) 824, 856
FatI CATG 5 cut(s) 190, 244, 433, 447, 1027
FblI GTMKAC 1 cut(s) 913
Fnu4HI GCNGC 1 cut(s) 135
FokI GGATG 2 cut(s) 539, 656
FriOI GRGCYC 1 cut(s) 732
Fsp4HI GCNGC 1 cut(s) 135
FspBI CTAG 3 cut(s) 14, 486, 950
FspI TGCGCA 1 cut(s) 679
GlaI GCGC 2 cut(s) 543, 679
GluI GCNGC 1 cut(s) 135
HaeIII GGCC 3 cut(s) 134, 686, 694
HapII CCGG 4 cut(s) 210, 545, 687, 725
HhaI GCGC 2 cut(s) 544, 680
Hin1II CATG 5 cut(s) 194, 248, 437, 451, 1031
Hin6I GCGC 2 cut(s) 542, 678
HinP1I GCGC 2 cut(s) 542, 678
HincII GTYRAC 1 cut(s) 914
HindII GTYRAC 1 cut(s) 914
HindIII AAGCTT 1 cut(s) 170
HinfI GANTC 3 cut(s) 206, 322, 1024
HpaII CCGG 4 cut(s) 210, 545, 687, 725
HphI GGTGA 3 cut(s) 47, 262, 463
Hpy166II GTNNAC 1 cut(s) 914
Hpy188I TCNGA 2 cut(s) 363, 415
Hpy188III TCNNGA 4 cut(s) 605, 725, 895, 1028
Hpy8I GTNNAC 1 cut(s) 914
Hpy99I CGWCG 1 cut(s) 915
HpyAV CCTTC 4 cut(s) 155, 365, 502, 728
HpyCH4III ACNGT 1 cut(s) 586
HpyCH4IV ACGT 4 cut(s) 236, 970, 978, 1003
HpyCH4V TGCA 3 cut(s) 190, 653, 812
HpyF10VI GCNNNNNNNGC 4 cut(s) 191, 196, 714, 770
HpyF3I CTNAG 4 cut(s) 202, 318, 841, 858
HpySE526I ACGT 4 cut(s) 236, 970, 978, 1003
Hsp92II CATG 5 cut(s) 194, 248, 437, 451, 1031
HspAI GCGC 2 cut(s) 542, 678
Kpn2I TCCGGA 1 cut(s) 724
KroI GCCGGC 1 cut(s) 544
KroNI GCCGGC 1 cut(s) 546
Kzo9I GATC 4 cut(s) 61, 670, 766, 805
LmnI GCTCC 5 cut(s) 445, 703, 727, 796, 934
LweI GCATC 1 cut(s) 238
MaeI CTAG 3 cut(s) 14, 486, 950
MaeII ACGT 4 cut(s) 236, 970, 978, 1003
MaeIII GTNAC 4 cut(s) 178, 297, 401, 901
MalI GATC 4 cut(s) 63, 672, 768, 807
MboI GATC 4 cut(s) 61, 670, 766, 805
MboII GAAGA 6 cut(s) 50, 53, 59, 376, 751, 899
MflI RGATCY 1 cut(s) 805
MhlI GDGCHC 2 cut(s) 201, 732
MluCI AATT 6 cut(s) 340, 375, 394, 475, 521, 633
MlyI GAGTC 2 cut(s) 200, 1033
MreI CGCCGGCG 1 cut(s) 544
MroI TCCGGA 1 cut(s) 724
MroNI GCCGGC 1 cut(s) 544
MseI TTAA 1 cut(s) 474
MslI CAYNNNNRTG 1 cut(s) 185
MspA1I CMGCKG 1 cut(s) 22
MspI CCGG 4 cut(s) 210, 545, 687, 725
MspR9I CCNGG 2 cut(s) 759, 848
MvaI CCWGG 2 cut(s) 759, 848
MwoI GCNNNNNNNGC 4 cut(s) 191, 196, 714, 770
NaeI GCCGGC 1 cut(s) 546
NdeII GATC 4 cut(s) 61, 670, 766, 805
NgoMIV GCCGGC 1 cut(s) 544
NlaIII CATG 5 cut(s) 194, 248, 437, 451, 1031
NlaIV GGNNCC 2 cut(s) 441, 466
NmuCI GTSAC 2 cut(s) 178, 297
NsbI TGCGCA 1 cut(s) 679
NspI RCATGY 2 cut(s) 194, 437
OliI CACNNNNGTG 1 cut(s) 185
PaeI GCATGC 1 cut(s) 194
PagI TCATGA 1 cut(s) 1027
PciI ACATGT 1 cut(s) 433
PdiI GCCGGC 1 cut(s) 546
PfeI GAWTC 1 cut(s) 322
PkrI GCNGC 1 cut(s) 136
PleI GAGTC 2 cut(s) 200, 1032
PpsI GAGTC 2 cut(s) 200, 1032
PpuMI RGGWCCY 1 cut(s) 465
PscI ACATGT 1 cut(s) 433
PshBI ATTAAT 1 cut(s) 474
Psp124BI GAGCTC 1 cut(s) 732
Psp1406I AACGTT 1 cut(s) 978
Psp5II RGGWCCY 1 cut(s) 465
Psp6I CCWGG 2 cut(s) 757, 846
PspGI CCWGG 2 cut(s) 757, 846
PspN4I GGNNCC 2 cut(s) 441, 466
PspPI GGNCC 1 cut(s) 465
PspPPI RGGWCCY 1 cut(s) 465
PstNI CAGNNNCTG 1 cut(s) 764
PsuI RGATCY 1 cut(s) 805
RsaI GTAC 6 cut(s) 83, 89, 117, 235, 243, 874
RsaNI GTAC 6 cut(s) 82, 88, 116, 234, 242, 873
RseI CAYNNNNRTG 1 cut(s) 185
SacI GAGCTC 1 cut(s) 732
SalI GTCGAC 1 cut(s) 912
SaqAI TTAA 1 cut(s) 474
SatI GCNGC 1 cut(s) 135
Sau3AI GATC 4 cut(s) 61, 670, 766, 805
Sau96I GGNCC 1 cut(s) 465
ScaI AGTACT 1 cut(s) 89
SchI GAGTC 2 cut(s) 200, 1033
ScrFI CCNGG 2 cut(s) 759, 848
SduI GDGCHC 2 cut(s) 201, 732
SfaNI GCATC 1 cut(s) 238
SgrAI CRCCGGYG 1 cut(s) 544
SinI GGWCC 1 cut(s) 465
SmiMI CAYNNNNRTG 1 cut(s) 185
SpeI ACTAGT 1 cut(s) 949
SphI GCATGC 1 cut(s) 194
Sse9I AATT 6 cut(s) 340, 375, 394, 475, 521, 633
SsiI CCGC 3 cut(s) 22, 135, 185
SspMI CTAG 3 cut(s) 14, 486, 950
SstI GAGCTC 1 cut(s) 732
StyD4I CCNGG 2 cut(s) 757, 846
TaaI ACNGT 1 cut(s) 586
TaiI ACGT 4 cut(s) 239, 973, 981, 1006
TaqI TCGA 2 cut(s) 330, 913
TasI AATT 6 cut(s) 340, 375, 394, 475, 521, 633
TatI WGTACW 3 cut(s) 81, 87, 241
TauI GCSGC 1 cut(s) 137
TfiI GAWTC 1 cut(s) 322
Tru1I TTAA 1 cut(s) 474
Tru9I TTAA 1 cut(s) 474
TscAI CASTG 1 cut(s) 187
TseFI GTSAC 2 cut(s) 178, 297
Tsp45I GTSAC 2 cut(s) 178, 297
TspDTI ATGAA 2 cut(s) 752, 1044
TspRI CASTG 1 cut(s) 187
VpaK11BI GGWCC 1 cut(s) 465
VspI ATTAAT 1 cut(s) 474
XapI RAATTY 1 cut(s) 394
XceI RCATGY 2 cut(s) 194, 437
XmiI GTMKAC 1 cut(s) 913
XspI CTAG 3 cut(s) 14, 486, 950
ZrmI AGTACT 1 cut(s) 89
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.