Rorug04G0079100

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0000910
Physical Location & Seq
Forward (+)
12134983 .. 12136645
1663 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0079100.1

Sequence Viewer

Length: 402 bp
ATGCCTTTCAAGAGGTACGTGGAGATCGGAAGGGTGGCTCTCGTCAACTACGGCGAGGACTACGGCAAGCTCGTCGTCATCGTCGACGTCATCGACCAGAACAGAGCTCTTGTTGATGCCCCTGATATGGTGAGATCTCAAATGAATTTCAAGAGGCTTTCTCTCACTGACATCAAAATTGACATCAAGAGGGTCCCCAAGAAGAAGGAATTGCTTGCTGCAATGGAGGCTGCTGATGTTAAGAAGAAGTGGGAAAGTAGCTCTTGGGGTAGGAAGCTTATTGTTCAGAAGCGAAGGGCCAATCTTAATGACTTTGATCGCTTCAAGCTTATGTTGGCTAAGATCAAGAGGGCTGGCCTGGTCAAGAGCGAACTTGCAAAACTGAAGAAGCAGAGTGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like HLH UN_NPL4

Protein Analysis

133

Amino Acids

15.33

Weight (kDa)

10.18

Isoelectric Point (pI)

32.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L14e PF01929 45 - 117 2.1e-26 Ribosomal protein L14
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 90
AccI GTMKAC 1 cut(s) 84
AcsI RAATTY 1 cut(s) 145
AcyI GRCGYC 1 cut(s) 87
AfaI GTAC 1 cut(s) 17
AfiI CCNNNNNNNGG 1 cut(s) 127
AgsI TTSAA 3 cut(s) 10, 151, 325
AjnI CCWGG 1 cut(s) 357
AluBI AGCT 5 cut(s) 70, 107, 261, 277, 328
AluI AGCT 5 cut(s) 70, 107, 261, 277, 328
Alw21I GWGCWC 1 cut(s) 109
AoxI GGCC 2 cut(s) 297, 355
ApeKI GCWGC 2 cut(s) 218, 230
ApoI RAATTY 1 cut(s) 145
AspS9I GGNCC 2 cut(s) 193, 297
AsuHPI GGTGA 1 cut(s) 142
AvaII GGWCC 1 cut(s) 193
BanII GRGCYC 1 cut(s) 109
Bbv12I GWGCWC 1 cut(s) 109
BbvI GCAGC 2 cut(s) 205, 217
BceAI ACGGC 2 cut(s) 67, 79
BcgI CGANNNNNNTGC 2 cut(s) 55, 89
BciT130I CCWGG 1 cut(s) 359
BfaI CTAG 1 cut(s) 400
BglII AGATCT 1 cut(s) 134
BisI GCNGC 2 cut(s) 219, 231
BlsI GCNGC 2 cut(s) 220, 232
Bme1390I CCNGG 1 cut(s) 359
Bme18I GGWCC 1 cut(s) 193
BmgT120I GGNCC 2 cut(s) 193, 297
BmiI GGNNCC 2 cut(s) 194, 195
BmrFI CCNGG 1 cut(s) 359
BmsI GCATC 1 cut(s) 106
BplI GAGNNNNNCTC 2 cut(s) 145, 177
BsaAI YACGTR 1 cut(s) 19
BsaHI GRCGYC 1 cut(s) 87
Bsc4I CCNNNNNNNGG 1 cut(s) 127
Bse3DI GCAATG 1 cut(s) 228
BseBI CCWGG 1 cut(s) 359
BseLI CCNNNNNNNGG 1 cut(s) 127
BseMI GCAATG 1 cut(s) 228
BseXI GCAGC 2 cut(s) 205, 217
BshFI GGCC 2 cut(s) 299, 357
BsiHKAI GWGCWC 1 cut(s) 109
BslFI GGGAC 1 cut(s) 179
BslI CCNNNNNNNGG 1 cut(s) 127
BsmFI GGGAC 1 cut(s) 179
BsnI GGCC 2 cut(s) 299, 357
Bsp1286I GDGCHC 1 cut(s) 109
Bsp143I GATC 4 cut(s) 24, 134, 316, 342
BspANI GGCC 2 cut(s) 299, 357
BspLI GGNNCC 2 cut(s) 194, 195
BsrDI GCAATG 1 cut(s) 228
BssMI GATC 4 cut(s) 24, 134, 316, 342
BssNI GRCGYC 1 cut(s) 87
Bst2UI CCWGG 1 cut(s) 359
BstACI GRCGYC 1 cut(s) 87
BstBAI YACGTR 1 cut(s) 19
BstC8I GCNNGC 3 cut(s) 68, 216, 355
BstDEI CTNAG 1 cut(s) 339
BstKTI GATC 4 cut(s) 27, 137, 319, 345
BstMBI GATC 4 cut(s) 24, 134, 316, 342
BstMWI GCNNNNNNNGC 1 cut(s) 227
BstNI CCWGG 1 cut(s) 359
BstSCI CCNGG 1 cut(s) 357
BstV1I GCAGC 2 cut(s) 205, 217
BstX2I RGATCY 1 cut(s) 134
BstYI RGATCY 1 cut(s) 134
BsuRI GGCC 2 cut(s) 299, 357
BtsIMutI CAGTG 1 cut(s) 165
Cac8I GCNNGC 3 cut(s) 68, 216, 355
Cfr13I GGNCC 2 cut(s) 193, 297
Csp6I GTAC 1 cut(s) 16
CviQI GTAC 1 cut(s) 16
DdeI CTNAG 1 cut(s) 339
DpnI GATC 4 cut(s) 26, 136, 318, 344
DpnII GATC 4 cut(s) 24, 134, 316, 342
Ecl136II GAGCTC 1 cut(s) 107
Eco24I GRGCYC 1 cut(s) 109
Eco47I GGWCC 1 cut(s) 193
Eco53kI GAGCTC 1 cut(s) 107
EcoICRI GAGCTC 1 cut(s) 107
EcoO109I RGGNCCY 1 cut(s) 193
EcoRII CCWGG 1 cut(s) 357
EcoT38I GRGCYC 1 cut(s) 109
FaiI YATR 2 cut(s) 128, 332
FalI AAGNNNNNCTT 2 cut(s) 247, 279
FaqI GGGAC 1 cut(s) 179
FblI GTMKAC 1 cut(s) 84
Fnu4HI GCNGC 2 cut(s) 219, 231
FriOI GRGCYC 1 cut(s) 109
Fsp4HI GCNGC 2 cut(s) 219, 231
FspBI CTAG 1 cut(s) 400
GluI GCNGC 2 cut(s) 219, 231
HaeIII GGCC 2 cut(s) 299, 357
Hin1I GRCGYC 1 cut(s) 87
HincII GTYRAC 2 cut(s) 46, 85
HindII GTYRAC 2 cut(s) 46, 85
HindIII AAGCTT 2 cut(s) 275, 326
HphI GGTGA 1 cut(s) 142
Hpy166II GTNNAC 2 cut(s) 46, 85
Hpy188I TCNGA 2 cut(s) 29, 288
Hpy188III TCNNGA 5 cut(s) 10, 151, 187, 346, 364
Hpy8I GTNNAC 2 cut(s) 46, 85
Hpy99I CGWCG 3 cut(s) 77, 86, 89
HpyAV CCTTC 3 cut(s) 24, 199, 288
HpyCH4IV ACGT 2 cut(s) 18, 87
HpyCH4V TGCA 2 cut(s) 221, 377
HpyF10VI GCNNNNNNNGC 1 cut(s) 227
HpyF3I CTNAG 1 cut(s) 339
HpySE526I ACGT 2 cut(s) 18, 87
Hsp92I GRCGYC 1 cut(s) 87
KflI GGGWCCC 1 cut(s) 193
Kzo9I GATC 4 cut(s) 24, 134, 316, 342
LpnPI CCDG 5 cut(s) 110, 135, 339, 344, 371
Lsp1109I GCAGC 2 cut(s) 205, 217
LweI GCATC 1 cut(s) 106
MaeI CTAG 1 cut(s) 400
MaeII ACGT 2 cut(s) 18, 87
MalI GATC 4 cut(s) 26, 136, 318, 344
MboI GATC 4 cut(s) 24, 134, 316, 342
MboII GAAGA 3 cut(s) 214, 256, 397
MflI RGATCY 1 cut(s) 134
MhlI GDGCHC 1 cut(s) 109
MluCI AATT 3 cut(s) 145, 177, 209
MnlI CCTC 6 cut(s) 6, 49, 147, 183, 220, 342
MseI TTAA 2 cut(s) 240, 306
MspR9I CCNGG 1 cut(s) 359
MvaI CCWGG 1 cut(s) 359
MwoI GCNNNNNNNGC 1 cut(s) 227
NdeII GATC 4 cut(s) 24, 134, 316, 342
NlaIV GGNNCC 2 cut(s) 194, 195
PcsI WCGNNNNNNNCGW 4 cut(s) 69, 78, 81, 90
PkrI GCNGC 2 cut(s) 220, 232
Ppu21I YACGTR 1 cut(s) 19
PpuMI RGGWCCY 1 cut(s) 193
Psp124BI GAGCTC 1 cut(s) 109
Psp5II RGGWCCY 1 cut(s) 193
Psp6I CCWGG 1 cut(s) 357
PspGI CCWGG 1 cut(s) 357
PspN4I GGNNCC 2 cut(s) 194, 195
PspPI GGNCC 2 cut(s) 193, 297
PspPPI RGGWCCY 1 cut(s) 193
PsuI RGATCY 1 cut(s) 134
RsaI GTAC 1 cut(s) 17
RsaNI GTAC 1 cut(s) 16
SacI GAGCTC 1 cut(s) 109
SalI GTCGAC 1 cut(s) 83
SaqAI TTAA 2 cut(s) 240, 306
SatI GCNGC 2 cut(s) 219, 231
Sau3AI GATC 4 cut(s) 24, 134, 316, 342
Sau96I GGNCC 2 cut(s) 193, 297
ScrFI CCNGG 1 cut(s) 359
SduI GDGCHC 1 cut(s) 109
SetI ASST 8 cut(s) 17, 21, 72, 90, 109, 263, 279, 330
SfaNI GCATC 1 cut(s) 106
SgrDI CGTCGACG 1 cut(s) 83
SinI GGWCC 1 cut(s) 193
Sse9I AATT 3 cut(s) 145, 177, 209
SspMI CTAG 1 cut(s) 400
SstI GAGCTC 1 cut(s) 109
StyD4I CCNGG 1 cut(s) 357
TaiI ACGT 2 cut(s) 21, 90
TaqI TCGA 2 cut(s) 84, 93
TasI AATT 3 cut(s) 145, 177, 209
Tru1I TTAA 2 cut(s) 240, 306
Tru9I TTAA 2 cut(s) 240, 306
TscAI CASTG 1 cut(s) 172
TseI GCWGC 2 cut(s) 218, 230
TspDTI ATGAA 1 cut(s) 158
TspRI CASTG 1 cut(s) 172
VpaK11BI GGWCC 1 cut(s) 193
XapI RAATTY 1 cut(s) 145
XmiI GTMKAC 1 cut(s) 84
XspI CTAG 1 cut(s) 400
ZraI GACGTC 1 cut(s) 88
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.