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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccI | GTMKAC | 1 cut(s) | 50 |
| AcsI | RAATTY | 1 cut(s) | 28 |
| AgsI | TTSAA | 1 cut(s) | 99 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 129 |
| Alw26I | GTCTC | 3 cut(s) | 28, 90, 116 |
| ApoI | RAATTY | 1 cut(s) | 28 |
| BcoDI | GTCTC | 3 cut(s) | 28, 90, 116 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 129 |
| BsaWI | WCCGGW | 1 cut(s) | 115 |
| BseGI | GGATG | 1 cut(s) | 10 |
| BsiSI | CCGG | 1 cut(s) | 116 |
| BsmAI | GTCTC | 3 cut(s) | 28, 90, 116 |
| BsmBI | CGTCTC | 1 cut(s) | 116 |
| BstF5I | GGATG | 1 cut(s) | 10 |
| BstMAI | GTCTC | 3 cut(s) | 28, 90, 116 |
| BtsCI | GGATG | 1 cut(s) | 10 |
| DriI | GACNNNNNGTC | 1 cut(s) | 129 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 129 |
| Esp3I | CGTCTC | 1 cut(s) | 116 |
| FaiI | YATR | 1 cut(s) | 66 |
| FblI | GTMKAC | 1 cut(s) | 50 |
| FokI | GGATG | 1 cut(s) | 17 |
| FspEI | CC | 5 cut(s) | 24, 55, 101, 107, 129 |
| HapII | CCGG | 1 cut(s) | 116 |
| HincII | GTYRAC | 1 cut(s) | 51 |
| HindII | GTYRAC | 1 cut(s) | 51 |
| HpaII | CCGG | 1 cut(s) | 116 |
| Hpy166II | GTNNAC | 2 cut(s) | 24, 51 |
| Hpy8I | GTNNAC | 2 cut(s) | 24, 51 |
| Hpy99I | CGWCG | 2 cut(s) | 55, 93 |
| HpyAV | CCTTC | 1 cut(s) | 63 |
| HpyCH4IV | ACGT | 2 cut(s) | 125, 130 |
| HpySE526I | ACGT | 2 cut(s) | 125, 130 |
| LpnPI | CCDG | 1 cut(s) | 129 |
| MaeII | ACGT | 2 cut(s) | 125, 130 |
| MaeIII | GTNAC | 1 cut(s) | 126 |
| MluCI | AATT | 1 cut(s) | 28 |
| MseI | TTAA | 1 cut(s) | 138 |
| MslI | CAYNNNNRTG | 1 cut(s) | 99 |
| MspI | CCGG | 1 cut(s) | 116 |
| RseI | CAYNNNNRTG | 1 cut(s) | 99 |
| SalI | GTCGAC | 1 cut(s) | 49 |
| SaqAI | TTAA | 1 cut(s) | 138 |
| SetI | ASST | 3 cut(s) | 74, 128, 133 |
| SgeI | CNNG | 5 cut(s) | 29, 50, 94, 99, 128 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 99 |
| Sse9I | AATT | 1 cut(s) | 28 |
| TaiI | ACGT | 2 cut(s) | 128, 133 |
| TaqI | TCGA | 1 cut(s) | 50 |
| TasI | AATT | 1 cut(s) | 28 |
| Tru1I | TTAA | 1 cut(s) | 138 |
| Tru9I | TTAA | 1 cut(s) | 138 |
| XapI | RAATTY | 1 cut(s) | 28 |
| XmiI | GTMKAC | 1 cut(s) | 50 |