MD08G1226500.v1.1
ERF Family

DNA RNA polymerases superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0000370
Physical Location & Seq
Reverse (-)
29286039 .. 29291770
5732 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1226500.v1.1.491

Sequence Viewer

Length: 1263 bp
ATGCAAAGGCATGAGAATTTCCCTGAGGATAGATGGGTAGAGATGACTACTTGGTTTATAGGTGAGGAGGTTGCATCCTGGTGGAGGAAGGAATCTTTTCAGTTGTCACTAGAAGAAGCCGCAGACTGGGAGGTTTTTAAGCAGCTATTCCAGAAGAGATTTGTACCTCCAGAGTATATAGATCGTAAGAAGCAAGAATTCACGCATCTAAAGCAAGGGAAAATGTCAACTAATGAGTACTATGGAAAGTTCATTGATTTATCTCGGTATTGTCCGGAGATTGCTGAGAATCCTGCTGAGATGCTTCGACAATTCAAGTGGGGTACTCGAAAGAAGTGGCGTTCTATAGCGACCACGATTCCCTGCTCTACATGTCAGGAGTTTTATGAGATTCTACTGCGGGTTGAGGATTCTGAAATGATGCCCAGTGATAGTGAGGAAGAGGAAGAAAAAGATAATAATCAGAAGAAAAACAATAATAAAGATAAAGGTCAGTCATTACAGGGACCTCGTAAAACGCAGAGTTTTAAGAGAAGTGGTATTAGTTCTGGCTCTTCTAGTGGGGGTTCGAGTTCCACGGCACCGTGGAGGGGTGGTCATTCAGGAGGTTCCCGCTTTCAGAGACAGAGGGATTTTAGTGGTTCTGGTGATCCATTCTGCCATAGGTGTAATGGTCAACATTTTAGGGATTGCAGACAGGGCAGCAGAAGATGTTTTGTTTGTGGTCGGACGGGACATCGGGCTGTTCAATGCCCACAGAACCAACAGAAATCCCAGCCACCTGCCTTACCACCAGCAGTTCCACTTCAGCAGGTCCCAGGGCCTAGTGCTTATACCTCGACGAGTTATGGGGGTGCATATCATTATCAGGGAGATATAGCTCCATATTTTGGAGGACAATATCAGTACCCACACGATCTGTATCATCAGAGTGGTTATGCTCAGTATCCAGGAGGTTACACACCATATCCACCATATTTAGGTGGTGGATCACAGTGGTATCCTGGATGGCAGTCCCAGAACGTTGAGGTTGCTTCTAGCAGTGCAGGATCATCGAGGCAGCCTAATCAGCCTTGTCAGGGACGTGGAAATCAGACTAATGGAGGTCGCAGAGGACGACAACAGCCACATGGCCATGTTCACCACATTACTCTGCAGGATGCGTGGAGTAATCCTGACCTTATCATGAGCGAGATGTTCCAAGAGGACAAGCGTAGCGAGGCGAGACTTGGGGGCTATGACCCTGCTACATACCAGTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVP_2 RVT_1 Retrotrans_gag rve zf-CCHC

Protein Analysis

421

Amino Acids

48.0

Weight (kDa)

8.55

Isoelectric Point (pI)

65.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Retrotrans_gag PF03732 20 - 108 5.1e-13 Retrotransposon gag protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000370)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g29481 FvH4_2g11454 FvH4_3g19661 FvH4_3g28463 FvH4_3g31691 FvH4_3g34551 FvH4_3g35241 FvH4_4g06575 FvH4_4g09082 FvH4_4g15804 FvH4_4g15805 FvH4_4g16178 FvH4_4g16282 FvH4_5g25163 FvH4_6g27413 FvH4_7g02962 FvH4_7g04361
malus_domestica MD01G1095000.v1.1 MD02G1270200.v1.1 MD02G1270300.v1.1 MD02G1270400.v1.1 MD06G1059100.v1.1 MD08G1226500.v1.1
prunus_persica Prupe.1G169500_v2.0.a1 Prupe.5G195800_v2.0.a1
pyrus_communis pycom01g00010 pycom01g00770 pycom02g23140 pycom03g11760 pycom04g06090 pycom05g06900 pycom05g08070 pycom05g14500 pycom06g03050 pycom07g06250 pycom07g07540 pycom07g10580 pycom08g04990 pycom1146g00060 pycom11g01080 pycom11g15270 pycom11g15460 pycom11g24900 pycom12419g00150 pycom12424g00120 pycom13g08740 pycom13g24950 pycom13g25960 pycom13g26030 pycom13g27820 pycom16g23270 pycom16g25020 pycom16g25960 pycom17g07780 pycom17g08210 pycom17g13100
rosa_chinensis RchiOBHm_Chr1g0329421 RchiOBHm_Chr4g0397711 RchiOBHm_Chr4g0403481 RchiOBHm_Chr5g0019091 RchiOBHm_Chr5g0049101
rosa_laevigata RLG00000029892
rosa_multiflora Rmu_sc0001866.1_g000007
rosa_roxburghii Rroxscaffold_2G00094160 Rroxscaffold_3G00229110 Rroxscaffold_4G00321100 Rroxscaffold_7G00196040 Rroxscaffold_7G00196280
rosa_rugosa Rorug05G0160500 Rorug06G0096500
rosa_samantha Rh1AG098600 Rh1AG098800 Rh1BG078200 Rh1BG078700 Rh1BG178500 Rh1CG094600 Rh1CG094900 Rh1DG101700 Rh1DG109600 Rh2AG175800 Rh2AG175900 Rh2AG511400 Rh2BG114700 Rh2CG042200 Rh2CG116600 Rh2CG317500 Rh2DG327700 Rh2DG337800 Rh4CG142700 Rh4DG113100 Rh5BG335800 Rh5CG360600 Rh5DG352000 Rh6BG071400 Rh6BG417100 Rh6CG463300 Rh6CG463400 Rh7BG186600 Rh7CG196500 Rh7CG196600 Rh7CG448900 Rh7DG228300
rosa_wichuraiana Rw4G006560 Rw4G017340 Rw5G030450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 790
Acc36I ACCTGC 2 cut(s) 790, 802
AccB1I GGYRCC 1 cut(s) 580
AccB7I CCANNNNNTGG 1 cut(s) 890
AccIII TCCGGA 1 cut(s) 274
AciI CCGC 3 cut(s) 120, 400, 613
AclI AACGTT 1 cut(s) 1023
AclWI GGATC 3 cut(s) 644, 997, 1057
AcoI YGGCCR 1 cut(s) 1132
AcsI RAATTY 2 cut(s) 16, 197
AcuI CTGAAG 1 cut(s) 791
AfaI GTAC 4 cut(s) 165, 239, 325, 908
AfiI CCNNNNNNNGG 6 cut(s) 84, 126, 590, 890, 980, 1079
AflIII ACRYGT 1 cut(s) 371
AgsI TTSAA 2 cut(s) 316, 749
AjiI CACGTC 1 cut(s) 1085
AjnI CCWGG 4 cut(s) 77, 817, 949, 1003
AluBI AGCT 2 cut(s) 145, 881
AluI AGCT 2 cut(s) 145, 881
Alw26I GTCTC 2 cut(s) 616, 1219
AlwI GGATC 3 cut(s) 644, 997, 1057
Aor13HI TCCGGA 1 cut(s) 274
AoxI GGCC 2 cut(s) 821, 1132
ApeKI GCWGC 3 cut(s) 142, 702, 1060
ApoI RAATTY 2 cut(s) 16, 197
Asp700I GAANNNNTTC 1 cut(s) 96
AspS9I GGNCC 3 cut(s) 506, 814, 821
AsuHPI GGTGA 3 cut(s) 74, 659, 1133
AvaII GGWCC 2 cut(s) 506, 814
AxyI CCTNAGG 1 cut(s) 24
BaeI ACNNNNGTAYC 2 cut(s) 983, 1016
BalI TGGCCA 1 cut(s) 1134
BanI GGYRCC 1 cut(s) 580
BbvI GCAGC 3 cut(s) 154, 714, 1072
BccI CCATC 2 cut(s) 27, 1002
BceAI ACGGC 1 cut(s) 594
BcgI CGANNNNNNTGC 2 cut(s) 1035, 1069
BciT130I CCWGG 4 cut(s) 79, 819, 951, 1005
BciVI GTATCC 2 cut(s) 957, 1011
BcoDI GTCTC 2 cut(s) 616, 1219
BfaI CTAG 5 cut(s) 110, 558, 825, 1038, 1261
BfmI CTRYAG 2 cut(s) 345, 1154
BfuAI ACCTGC 2 cut(s) 790, 802
BfuI GTATCC 2 cut(s) 957, 1011
BisI GCNGC 4 cut(s) 120, 143, 703, 1061
BlsI GCNGC 4 cut(s) 121, 144, 704, 1062
BmcAI AGTACT 1 cut(s) 239
Bme1390I CCNGG 4 cut(s) 79, 819, 951, 1005
Bme18I GGWCC 2 cut(s) 506, 814
BmgBI CACGTC 1 cut(s) 1085
BmgT120I GGNCC 3 cut(s) 506, 814, 821
BmiI GGNNCC 4 cut(s) 507, 582, 610, 816
BmrFI CCNGG 4 cut(s) 79, 819, 951, 1005
BmrI ACTGGG 2 cut(s) 136, 420
BmsI GCATC 5 cut(s) 83, 214, 291, 411, 1150
BmuI ACTGGG 2 cut(s) 136, 420
BpmI CTGGAG 1 cut(s) 153
BsaBI GATNNNNATC 2 cut(s) 459, 921
BsaJI CCNNGG 4 cut(s) 576, 584, 817, 818
BsaWI WCCGGW 1 cut(s) 274
BsaXI ACNNNNNCTCC 2 cut(s) 580, 610
Bsc4I CCNNNNNNNGG 6 cut(s) 84, 126, 590, 890, 980, 1079
Bse1I ACTGG 3 cut(s) 131, 426, 1255
Bse21I CCTNAGG 1 cut(s) 24
Bse8I GATNNNNATC 2 cut(s) 459, 921
BseAI TCCGGA 1 cut(s) 274
BseBI CCWGG 4 cut(s) 79, 819, 951, 1005
BseDI CCNNGG 4 cut(s) 576, 584, 817, 818
BseGI GGATG 3 cut(s) 74, 1013, 1165
BseJI GATNNNNATC 2 cut(s) 459, 921
BseLI CCNNNNNNNGG 6 cut(s) 84, 126, 590, 890, 980, 1079
BseMII CTCAG 4 cut(s) 15, 276, 288, 956
BseNI ACTGG 3 cut(s) 131, 426, 1255
BseRI GAGGAG 1 cut(s) 80
BseXI GCAGC 3 cut(s) 154, 714, 1072
BseYI CCCAGC 1 cut(s) 774
BsgI GTGCAG 1 cut(s) 1065
BshFI GGCC 2 cut(s) 823, 1134
BshNI GGYRCC 1 cut(s) 580
BsiSI CCGG 1 cut(s) 275
BslFI GGGAC 5 cut(s) 519, 747, 800, 1000, 1095
BslI CCNNNNNNNGG 6 cut(s) 84, 126, 590, 890, 980, 1079
BsmAI GTCTC 2 cut(s) 616, 1219
BsmFI GGGAC 5 cut(s) 519, 747, 800, 1000, 1095
BsnI GGCC 2 cut(s) 823, 1134
Bsp13I TCCGGA 1 cut(s) 274
Bsp143I GATC 5 cut(s) 181, 649, 916, 989, 1049
BspACI CCGC 3 cut(s) 120, 400, 613
BspANI GGCC 2 cut(s) 823, 1134
BspCNI CTCAG 4 cut(s) 16, 277, 289, 955
BspEI TCCGGA 1 cut(s) 274
BspHI TCATGA 1 cut(s) 1185
BspLI GGNNCC 4 cut(s) 507, 582, 610, 816
BspMAI CTGCAG 1 cut(s) 1158
BspMI ACCTGC 2 cut(s) 790, 802
BspPI GGATC 3 cut(s) 644, 997, 1057
BspQI GCTCTTC 1 cut(s) 559
BspT107I GGYRCC 1 cut(s) 580
BsrI ACTGG 3 cut(s) 131, 426, 1255
BssECI CCNNGG 4 cut(s) 576, 584, 817, 818
BssMI GATC 5 cut(s) 181, 649, 916, 989, 1049
Bst2UI CCWGG 4 cut(s) 79, 819, 951, 1005
Bst4CI ACNGT 2 cut(s) 585, 996
Bst6I CTCTTC 3 cut(s) 149, 435, 559
BstDEI CTNAG 4 cut(s) 24, 285, 297, 942
BstDSI CCRYGG 2 cut(s) 576, 584
BstENI CCTNNNNNAGG 1 cut(s) 82
BstF5I GGATG 3 cut(s) 74, 1013, 1165
BstKTI GATC 5 cut(s) 184, 652, 919, 992, 1052
BstMAI GTCTC 2 cut(s) 616, 1219
BstMBI GATC 5 cut(s) 181, 649, 916, 989, 1049
BstMWI GCNNNNNNNGC 3 cut(s) 211, 699, 1069
BstNI CCWGG 4 cut(s) 79, 819, 951, 1005
BstNSI RCATGY 1 cut(s) 375
BstSCI CCNGG 4 cut(s) 77, 817, 949, 1003
BstSFI CTRYAG 2 cut(s) 345, 1154
BstV1I GCAGC 3 cut(s) 154, 714, 1072
Bsu36I CCTNAGG 1 cut(s) 24
BsuI GTATCC 2 cut(s) 957, 1011
BsuRI GGCC 2 cut(s) 823, 1134
BtgI CCRYGG 2 cut(s) 576, 584
BtrI CACGTC 1 cut(s) 1085
BtsCI GGATG 3 cut(s) 74, 1013, 1165
BtsI GCAGTG 1 cut(s) 1048
BtsIMutI CAGTG 3 cut(s) 433, 1001, 1048
BveI ACCTGC 2 cut(s) 790, 802
CciI TCATGA 1 cut(s) 1185
Cfr13I GGNCC 3 cut(s) 506, 814, 821
Csp6I GTAC 4 cut(s) 164, 238, 324, 907
CspCI CAANNNNNGTGG 2 cut(s) 299, 334
CviAII CATG 5 cut(s) 11, 372, 1130, 1136, 1186
CviQI GTAC 4 cut(s) 164, 238, 324, 907
DdeI CTNAG 4 cut(s) 24, 285, 297, 942
DpnI GATC 5 cut(s) 183, 651, 918, 991, 1051
DpnII GATC 5 cut(s) 181, 649, 916, 989, 1049
EaeI YGGCCR 1 cut(s) 1132
Eam1104I CTCTTC 3 cut(s) 149, 435, 559
EarI CTCTTC 3 cut(s) 149, 435, 559
Eco47I GGWCC 2 cut(s) 506, 814
Eco57I CTGAAG 1 cut(s) 791
Eco81I CCTNAGG 1 cut(s) 24
EcoNI CCTNNNNNAGG 1 cut(s) 82
EcoO109I RGGNCCY 3 cut(s) 506, 814, 821
EcoRI GAATTC 1 cut(s) 197
EcoRII CCWGG 4 cut(s) 77, 817, 949, 1003
FaeI CATG 5 cut(s) 14, 375, 1133, 1139, 1189
FaqI GGGAC 5 cut(s) 519, 747, 800, 1000, 1095
FatI CATG 5 cut(s) 10, 371, 1129, 1135, 1185
FauI CCCGC 2 cut(s) 393, 620
Fnu4HI GCNGC 4 cut(s) 120, 143, 703, 1061
FokI GGATG 3 cut(s) 61, 1020, 1172
Fsp4HI GCNGC 4 cut(s) 120, 143, 703, 1061
FspBI CTAG 5 cut(s) 110, 558, 825, 1038, 1261
GluI GCNGC 4 cut(s) 120, 143, 703, 1061
GsaI CCCAGC 1 cut(s) 778
GsuI CTGGAG 1 cut(s) 153
HaeIII GGCC 2 cut(s) 823, 1134
HapII CCGG 1 cut(s) 275
Hin1II CATG 5 cut(s) 14, 375, 1133, 1139, 1189
HincII GTYRAC 2 cut(s) 228, 677
HindII GTYRAC 2 cut(s) 228, 677
HinfI GANTC 5 cut(s) 92, 289, 358, 391, 410
HpaII CCGG 1 cut(s) 275
HphI GGTGA 3 cut(s) 74, 659, 1133
Hpy166II GTNNAC 3 cut(s) 228, 677, 1141
Hpy188I TCNGA 6 cut(s) 415, 465, 621, 729, 930, 1095
Hpy188III TCNNGA 7 cut(s) 151, 170, 275, 377, 603, 1175, 1186
Hpy8I GTNNAC 3 cut(s) 228, 677, 1141
Hpy99I CGWCG 1 cut(s) 844
HpyAV CCTTC 1 cut(s) 82
HpyCH4III ACNGT 2 cut(s) 585, 996
HpyCH4IV ACGT 2 cut(s) 1023, 1084
HpyCH4V TGCA 6 cut(s) 4, 74, 693, 857, 1046, 1156
HpyF10VI GCNNNNNNNGC 3 cut(s) 211, 699, 1069
HpyF3I CTNAG 4 cut(s) 24, 285, 297, 942
HpySE526I ACGT 2 cut(s) 1023, 1084
Hsp92II CATG 5 cut(s) 14, 375, 1133, 1139, 1189
Kpn2I TCCGGA 1 cut(s) 274
Kzo9I GATC 5 cut(s) 181, 649, 916, 989, 1049
LguI GCTCTTC 1 cut(s) 559
LmnI GCTCC 1 cut(s) 886
Lsp1109I GCAGC 3 cut(s) 154, 714, 1072
LweI GCATC 5 cut(s) 83, 214, 291, 411, 1150
MaeI CTAG 5 cut(s) 110, 558, 825, 1038, 1261
MaeII ACGT 2 cut(s) 1023, 1084
MaeIII GTNAC 2 cut(s) 105, 956
MalI GATC 5 cut(s) 183, 651, 918, 991, 1051
MboI GATC 5 cut(s) 181, 649, 916, 989, 1049
MboII GAAGA 7 cut(s) 125, 166, 452, 458, 478, 546, 720
MlsI TGGCCA 1 cut(s) 1134
MluCI AATT 3 cut(s) 16, 197, 311
MluNI TGGCCA 1 cut(s) 1134
MmeI TCCRAC 1 cut(s) 707
Mox20I TGGCCA 1 cut(s) 1134
MroI TCCGGA 1 cut(s) 274
MroXI GAANNNNTTC 1 cut(s) 96
MscI TGGCCA 1 cut(s) 1134
MseI TTAA 2 cut(s) 138, 528
MslI CAYNNNNRTG 3 cut(s) 79, 930, 1134
Msp20I TGGCCA 1 cut(s) 1134
MspI CCGG 1 cut(s) 275
MspR9I CCNGG 4 cut(s) 79, 819, 951, 1005
MvaI CCWGG 4 cut(s) 79, 819, 951, 1005
MwoI GCNNNNNNNGC 3 cut(s) 211, 699, 1069
NdeII GATC 5 cut(s) 181, 649, 916, 989, 1049
NlaIII CATG 5 cut(s) 14, 375, 1133, 1139, 1189
NlaIV GGNNCC 4 cut(s) 507, 582, 610, 816
NmuCI GTSAC 1 cut(s) 105
NspI RCATGY 1 cut(s) 375
PagI TCATGA 1 cut(s) 1185
PaqCI CACCTGC 1 cut(s) 790
PasI CCCWGGG 1 cut(s) 818
PciI ACATGT 1 cut(s) 371
PciSI GCTCTTC 1 cut(s) 559
PcsI WCGNNNNNNNCGW 1 cut(s) 1114
PdmI GAANNNNTTC 1 cut(s) 96
PfeI GAWTC 5 cut(s) 92, 289, 358, 391, 410
PflMI CCANNNNNTGG 1 cut(s) 890
PfoI TCCNGGA 2 cut(s) 949, 1003
PkrI GCNGC 4 cut(s) 121, 144, 704, 1062
PpuMI RGGWCCY 2 cut(s) 506, 814
PscI ACATGT 1 cut(s) 371
Psp1406I AACGTT 1 cut(s) 1023
Psp5II RGGWCCY 2 cut(s) 506, 814
Psp6I CCWGG 4 cut(s) 77, 817, 949, 1003
PspFI CCCAGC 1 cut(s) 774
PspGI CCWGG 4 cut(s) 77, 817, 949, 1003
PspN4I GGNNCC 4 cut(s) 507, 582, 610, 816
PspPI GGNCC 3 cut(s) 506, 814, 821
PspPPI RGGWCCY 2 cut(s) 506, 814
PstI CTGCAG 1 cut(s) 1158
RsaI GTAC 4 cut(s) 165, 239, 325, 908
RsaNI GTAC 4 cut(s) 164, 238, 324, 907
RseI CAYNNNNRTG 3 cut(s) 79, 930, 1134
SapI GCTCTTC 1 cut(s) 559
SaqAI TTAA 2 cut(s) 138, 528
SatI GCNGC 4 cut(s) 120, 143, 703, 1061
Sau3AI GATC 5 cut(s) 181, 649, 916, 989, 1049
Sau96I GGNCC 3 cut(s) 506, 814, 821
ScaI AGTACT 1 cut(s) 239
ScrFI CCNGG 4 cut(s) 79, 819, 951, 1005
SfaNI GCATC 5 cut(s) 83, 214, 291, 411, 1150
SfcI CTRYAG 2 cut(s) 345, 1154
SinI GGWCC 2 cut(s) 506, 814
SmiMI CAYNNNNRTG 3 cut(s) 79, 930, 1134
Sse9I AATT 3 cut(s) 16, 197, 311
SsiI CCGC 3 cut(s) 120, 400, 613
SspMI CTAG 5 cut(s) 110, 558, 825, 1038, 1261
StyD4I CCNGG 4 cut(s) 77, 817, 949, 1003
TaaI ACNGT 2 cut(s) 585, 996
TaiI ACGT 2 cut(s) 1026, 1087
TaqI TCGA 5 cut(s) 307, 328, 569, 839, 1055
TasI AATT 3 cut(s) 16, 197, 311
TatI WGTACW 1 cut(s) 237
TauI GCSGC 1 cut(s) 122
TfiI GAWTC 5 cut(s) 92, 289, 358, 391, 410
Tru1I TTAA 2 cut(s) 138, 528
Tru9I TTAA 2 cut(s) 138, 528
TscAI CASTG 3 cut(s) 433, 1001, 1048
TseFI GTSAC 1 cut(s) 105
TseI GCWGC 3 cut(s) 142, 702, 1060
Tsp45I GTSAC 1 cut(s) 105
TspDTI ATGAA 1 cut(s) 241
TspRI CASTG 3 cut(s) 433, 1001, 1048
Van91I CCANNNNNTGG 1 cut(s) 890
VpaK11BI GGWCC 2 cut(s) 506, 814
XagI CCTNNNNNAGG 1 cut(s) 82
XapI RAATTY 2 cut(s) 16, 197
XceI RCATGY 1 cut(s) 375
XcmI CCANNNNNNNNNTGG 1 cut(s) 668
XmnI GAANNNNTTC 1 cut(s) 96
XspI CTAG 5 cut(s) 110, 558, 825, 1038, 1261
ZrmI AGTACT 1 cut(s) 239
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.