Rh6BG071400

Kynurenine formamidase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0000370
Physical Location & Seq
Reverse (-)
10792344 .. 10816532
24189 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG071400.1

Sequence Viewer

Length: 642 bp
ATGGTAGATACCGATGATAAAAAGACAAGACGATTCATTGGAGGACTGAATTCCAATATTCGAAGAATGGTCACTCAACGTGGAATCACGTACGAGGAAGCTGTGGACAAAGCCCTAACTCAAGAGGAAGAAAATCAAAAATACCGCATGGAGAAGGAGCGAGAGAATGGCTTCCGAGGAAAGAGAAGTCATCCAATGTCTAACCAGAAAAGCGGACAACCATGGAAGCAGCAAACAAAGAGAGACGCATTTCAGAAGCCTACAACATTAACTCAACAAGGAAATGAGGTAGCAAGAGACCCAATTCGGTGTTTCAACTGTGGAGAAACGGGGCACATGTCTAATGCTTGTCCAAAGCCACGTCACCTTCCAAGATCTTGTTATAATTGTGGCAAGATGGGACATTTCTCAAATCAGTGCGATGCACCGAGGCAAAAAAATAACCCCCCACCTCGTCCTGTCCAACTAAATGCCATAGCTTGTGAGAACATCGTGATGGAAGCTTATGTTGACGCACCTGGTCACGTCTTCGACCATTACTACCATGCTGGCTTTGACGTCGATGCTCTAGACGTTGAAGTGTTAAATGGCCCTGCGCTGGTGATTGCTGTTCCCAGGGACAAGAACATAACTGGTATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cyclase

Protein Analysis

213

Amino Acids

24.19

Weight (kDa)

8.82

Isoelectric Point (pI)

47.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-CCHC PF00098 103 - 119 4.4e-07 Zinc knuckle
zf-CCHC PF00098 126 - 141 1.4e-06 Zinc knuckle
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000370)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g29481 FvH4_2g11454 FvH4_3g19661 FvH4_3g28463 FvH4_3g31691 FvH4_3g34551 FvH4_3g35241 FvH4_4g06575 FvH4_4g09082 FvH4_4g15804 FvH4_4g15805 FvH4_4g16178 FvH4_4g16282 FvH4_5g25163 FvH4_6g27413 FvH4_7g02962 FvH4_7g04361
malus_domestica MD01G1095000.v1.1 MD02G1270200.v1.1 MD02G1270300.v1.1 MD02G1270400.v1.1 MD06G1059100.v1.1 MD08G1226500.v1.1
prunus_persica Prupe.1G169500_v2.0.a1 Prupe.5G195800_v2.0.a1
pyrus_communis pycom01g00010 pycom01g00770 pycom02g23140 pycom03g11760 pycom04g06090 pycom05g06900 pycom05g08070 pycom05g14500 pycom06g03050 pycom07g06250 pycom07g07540 pycom07g10580 pycom08g04990 pycom1146g00060 pycom11g01080 pycom11g15270 pycom11g15460 pycom11g24900 pycom12419g00150 pycom12424g00120 pycom13g08740 pycom13g24950 pycom13g25960 pycom13g26030 pycom13g27820 pycom16g23270 pycom16g25020 pycom16g25960 pycom17g07780 pycom17g08210 pycom17g13100
rosa_chinensis RchiOBHm_Chr1g0329421 RchiOBHm_Chr4g0397711 RchiOBHm_Chr4g0403481 RchiOBHm_Chr5g0019091 RchiOBHm_Chr5g0049101
rosa_laevigata RLG00000029892
rosa_multiflora Rmu_sc0001866.1_g000007
rosa_roxburghii Rroxscaffold_2G00094160 Rroxscaffold_3G00229110 Rroxscaffold_4G00321100 Rroxscaffold_7G00196040 Rroxscaffold_7G00196280
rosa_rugosa Rorug05G0160500 Rorug06G0096500
rosa_samantha Rh1AG098600 Rh1AG098800 Rh1BG078200 Rh1BG078700 Rh1BG178500 Rh1CG094600 Rh1CG094900 Rh1DG101700 Rh1DG109600 Rh2AG175800 Rh2AG175900 Rh2AG511400 Rh2BG114700 Rh2CG042200 Rh2CG116600 Rh2CG317500 Rh2DG327700 Rh2DG337800 Rh4CG142700 Rh4DG113100 Rh5BG335800 Rh5CG360600 Rh5DG352000 Rh6BG071400 Rh6BG417100 Rh6CG463300 Rh6CG463400 Rh7BG186600 Rh7CG196500 Rh7CG196600 Rh7CG448900 Rh7DG228300
rosa_wichuraiana Rw4G006560 Rw4G017340 Rw5G030450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 384
AatII GACGTC 1 cut(s) 561
AciI CCGC 2 cut(s) 145, 213
AcsI RAATTY 1 cut(s) 49
AcyI GRCGYC 1 cut(s) 558
AfaI GTAC 1 cut(s) 92
AfiI CCNNNNNNNGG 1 cut(s) 598
AflIII ACRYGT 1 cut(s) 336
AgsI TTSAA 2 cut(s) 316, 578
AjiI CACGTC 2 cut(s) 362, 526
AjnI CCWGG 2 cut(s) 517, 614
AluBI AGCT 3 cut(s) 101, 479, 503
AluI AGCT 3 cut(s) 101, 479, 503
Alw26I GTCTC 2 cut(s) 237, 291
AoxI GGCC 1 cut(s) 589
ApeKI GCWGC 1 cut(s) 229
ApoI RAATTY 1 cut(s) 49
Asp700I GAANNNNTTC 1 cut(s) 170
AspLEI GCGC 1 cut(s) 598
AspS9I GGNCC 1 cut(s) 590
AsuHPI GGTGA 2 cut(s) 356, 613
AsuII TTCGAA 1 cut(s) 61
BaeGI GKGCMC 1 cut(s) 336
BbsI GAAGAC 1 cut(s) 520
BbvI GCAGC 1 cut(s) 241
BccI CCATC 2 cut(s) 391, 490
BciT130I CCWGG 2 cut(s) 519, 616
BcoDI GTCTC 2 cut(s) 237, 291
BfaI CTAG 1 cut(s) 569
BglII AGATCT 1 cut(s) 374
BisI GCNGC 1 cut(s) 230
BlsI GCNGC 1 cut(s) 231
Bme1390I CCNGG 2 cut(s) 519, 616
BmgBI CACGTC 2 cut(s) 362, 526
BmgT120I GGNCC 1 cut(s) 590
BmrFI CCNGG 2 cut(s) 519, 616
BmsI GCATC 2 cut(s) 412, 553
BpiI GAAGAC 1 cut(s) 520
Bpu14I TTCGAA 1 cut(s) 61
BpuEI CTTGAG 1 cut(s) 105
BsaAI YACGTR 1 cut(s) 90
BsaHI GRCGYC 1 cut(s) 558
BsaI GGTCTC 1 cut(s) 291
BsaJI CCNNGG 5 cut(s) 175, 221, 428, 614, 615
Bsc4I CCNNNNNNNGG 1 cut(s) 598
Bse1I ACTGG 1 cut(s) 637
BseBI CCWGG 2 cut(s) 519, 616
BseDI CCNNGG 5 cut(s) 175, 221, 428, 614, 615
BseGI GGATG 1 cut(s) 190
BseLI CCNNNNNNNGG 1 cut(s) 598
BseNI ACTGG 1 cut(s) 637
BseSI GKGCMC 1 cut(s) 336
BseXI GCAGC 1 cut(s) 241
BshFI GGCC 1 cut(s) 591
BsiWI CGTACG 1 cut(s) 90
BslFI GGGAC 2 cut(s) 414, 632
BslI CCNNNNNNNGG 1 cut(s) 598
BsmAI GTCTC 2 cut(s) 237, 291
BsmBI CGTCTC 1 cut(s) 237
BsmFI GGGAC 2 cut(s) 414, 632
BsnI GGCC 1 cut(s) 591
Bso31I GGTCTC 1 cut(s) 291
Bsp119I TTCGAA 1 cut(s) 61
Bsp1286I GDGCHC 1 cut(s) 336
Bsp143I GATC 1 cut(s) 374
Bsp19I CCATGG 1 cut(s) 221
BspACI CCGC 2 cut(s) 145, 213
BspANI GGCC 1 cut(s) 591
BspT104I TTCGAA 1 cut(s) 61
BspTNI GGTCTC 1 cut(s) 291
BsrI ACTGG 1 cut(s) 637
BssECI CCNNGG 5 cut(s) 175, 221, 428, 614, 615
BssMI GATC 1 cut(s) 374
BssNI GRCGYC 1 cut(s) 558
BssT1I CCWWGG 1 cut(s) 221
Bst2UI CCWGG 2 cut(s) 519, 616
Bst4CI ACNGT 1 cut(s) 320
BstACI GRCGYC 1 cut(s) 558
BstBAI YACGTR 1 cut(s) 90
BstBI TTCGAA 1 cut(s) 61
BstC8I GCNNGC 1 cut(s) 550
BstDSI CCRYGG 1 cut(s) 221
BstF5I GGATG 1 cut(s) 190
BstHHI GCGC 1 cut(s) 598
BstKTI GATC 1 cut(s) 377
BstMAI GTCTC 2 cut(s) 237, 291
BstMBI GATC 1 cut(s) 374
BstNI CCWGG 2 cut(s) 519, 616
BstNSI RCATGY 1 cut(s) 340
BstSCI CCNGG 2 cut(s) 517, 614
BstSLI GKGCMC 1 cut(s) 336
BstV1I GCAGC 1 cut(s) 241
BstV2I GAAGAC 1 cut(s) 520
BstX2I RGATCY 1 cut(s) 374
BstYI RGATCY 1 cut(s) 374
BsuRI GGCC 1 cut(s) 591
BtgI CCRYGG 1 cut(s) 221
BtgZI GCGATG 1 cut(s) 435
BtrI CACGTC 2 cut(s) 362, 526
BtsCI GGATG 1 cut(s) 190
BtsIMutI CAGTG 1 cut(s) 422
Cac8I GCNNGC 1 cut(s) 550
CfoI GCGC 1 cut(s) 598
Cfr13I GGNCC 1 cut(s) 590
CseI GACGC 2 cut(s) 254, 521
CsiI ACCWGGT 1 cut(s) 517
Csp6I GTAC 1 cut(s) 91
CviAII CATG 4 cut(s) 148, 222, 337, 545
CviJI RGCY 9 cut(s) 101, 113, 171, 259, 358, 479, 503, 552, 591
CviKI_1 RGCY 9 cut(s) 101, 113, 171, 259, 358, 479, 503, 552, 591
CviQI GTAC 1 cut(s) 91
DpnI GATC 1 cut(s) 376
DpnII GATC 1 cut(s) 374
Eco130I CCWWGG 1 cut(s) 221
Eco31I GGTCTC 1 cut(s) 291
EcoRI GAATTC 1 cut(s) 49
EcoRII CCWGG 2 cut(s) 517, 614
EcoT14I CCWWGG 1 cut(s) 221
ErhI CCWWGG 1 cut(s) 221
Esp3I CGTCTC 1 cut(s) 237
FaeI CATG 4 cut(s) 151, 225, 340, 548
FaiI YATR 9 cut(s) 149, 223, 338, 384, 476, 507, 546, 629, 638
FaqI GGGAC 2 cut(s) 414, 632
FatI CATG 4 cut(s) 147, 221, 336, 544
Fnu4HI GCNGC 1 cut(s) 230
FokI GGATG 1 cut(s) 177
Fsp4HI GCNGC 1 cut(s) 230
FspBI CTAG 1 cut(s) 569
GlaI GCGC 1 cut(s) 597
GluI GCNGC 1 cut(s) 230
HaeIII GGCC 1 cut(s) 591
HgaI GACGC 2 cut(s) 254, 521
HhaI GCGC 1 cut(s) 598
Hin1I GRCGYC 1 cut(s) 558
Hin1II CATG 4 cut(s) 151, 225, 340, 548
Hin6I GCGC 1 cut(s) 596
HinP1I GCGC 1 cut(s) 596
HincII GTYRAC 1 cut(s) 511
HindII GTYRAC 1 cut(s) 511
HindIII AAGCTT 1 cut(s) 501
HinfI GANTC 2 cut(s) 33, 84
HphI GGTGA 2 cut(s) 356, 613
Hpy166II GTNNAC 2 cut(s) 106, 511
Hpy188I TCNGA 2 cut(s) 176, 255
Hpy188III TCNNGA 3 cut(s) 122, 493, 569
Hpy8I GTNNAC 2 cut(s) 106, 511
Hpy99I CGWCG 1 cut(s) 563
HpyAV CCTTC 2 cut(s) 148, 377
HpyCH4III ACNGT 1 cut(s) 320
HpyCH4IV ACGT 6 cut(s) 79, 89, 361, 525, 558, 573
HpyCH4V TGCA 1 cut(s) 425
HpySE526I ACGT 6 cut(s) 79, 89, 361, 525, 558, 573
Hsp92I GRCGYC 1 cut(s) 558
Hsp92II CATG 4 cut(s) 151, 225, 340, 548
HspAI GCGC 1 cut(s) 596
Kzo9I GATC 1 cut(s) 374
LmnI GCTCC 1 cut(s) 157
Lsp1109I GCAGC 1 cut(s) 241
LweI GCATC 2 cut(s) 412, 553
MabI ACCWGGT 1 cut(s) 517
MaeI CTAG 1 cut(s) 569
MaeII ACGT 6 cut(s) 79, 89, 361, 525, 558, 573
MaeIII GTNAC 3 cut(s) 70, 362, 521
MalI GATC 1 cut(s) 376
MboI GATC 1 cut(s) 374
MboII GAAGA 3 cut(s) 75, 140, 520
MflI RGATCY 1 cut(s) 374
MhlI GDGCHC 1 cut(s) 336
MluCI AATT 3 cut(s) 49, 303, 385
MmeI TCCRAC 1 cut(s) 487
MnlI CCTC 7 cut(s) 35, 88, 118, 170, 280, 423, 462
MroXI GAANNNNTTC 1 cut(s) 170
MseI TTAA 2 cut(s) 269, 584
MslI CAYNNNNRTG 1 cut(s) 494
MspR9I CCNGG 2 cut(s) 519, 616
MvaI CCWGG 2 cut(s) 519, 616
NcoI CCATGG 1 cut(s) 221
NdeII GATC 1 cut(s) 374
NlaIII CATG 4 cut(s) 151, 225, 340, 548
NmuCI GTSAC 3 cut(s) 70, 362, 521
NspI RCATGY 1 cut(s) 340
NspV TTCGAA 1 cut(s) 61
PasI CCCWGGG 1 cut(s) 615
PciI ACATGT 1 cut(s) 336
PdmI GAANNNNTTC 1 cut(s) 170
PfeI GAWTC 2 cut(s) 33, 84
Pfl23II CGTACG 1 cut(s) 90
PkrI GCNGC 1 cut(s) 231
Ppu21I YACGTR 1 cut(s) 90
PscI ACATGT 1 cut(s) 336
PsiI TTATAA 1 cut(s) 384
Psp6I CCWGG 2 cut(s) 517, 614
PspGI CCWGG 2 cut(s) 517, 614
PspLI CGTACG 1 cut(s) 90
PspPI GGNCC 1 cut(s) 590
PsuI RGATCY 1 cut(s) 374
RsaI GTAC 1 cut(s) 92
RsaNI GTAC 1 cut(s) 91
RseI CAYNNNNRTG 1 cut(s) 494
SaqAI TTAA 2 cut(s) 269, 584
SatI GCNGC 1 cut(s) 230
Sau3AI GATC 1 cut(s) 374
Sau96I GGNCC 1 cut(s) 590
ScrFI CCNGG 2 cut(s) 519, 616
SduI GDGCHC 1 cut(s) 336
SexAI ACCWGGT 1 cut(s) 517
SfaNI GCATC 2 cut(s) 412, 553
SfuI TTCGAA 1 cut(s) 61
SmiMI CAYNNNNRTG 1 cut(s) 494
SmlI CTYRAG 1 cut(s) 120
SmoI CTYRAG 1 cut(s) 120
Sse9I AATT 3 cut(s) 49, 303, 385
SsiI CCGC 2 cut(s) 145, 213
SspI AATATT 1 cut(s) 58
SspMI CTAG 1 cut(s) 569
StyD4I CCNGG 2 cut(s) 517, 614
StyI CCWWGG 1 cut(s) 221
TaaI ACNGT 1 cut(s) 320
TaiI ACGT 6 cut(s) 82, 92, 364, 528, 561, 576
TaqI TCGA 3 cut(s) 61, 531, 561
TasI AATT 3 cut(s) 49, 303, 385
TfiI GAWTC 2 cut(s) 33, 84
Tru1I TTAA 2 cut(s) 269, 584
Tru9I TTAA 2 cut(s) 269, 584
TscAI CASTG 1 cut(s) 422
TseFI GTSAC 3 cut(s) 70, 362, 521
TseI GCWGC 1 cut(s) 229
Tsp45I GTSAC 3 cut(s) 70, 362, 521
TspDTI ATGAA 1 cut(s) 25
TspRI CASTG 1 cut(s) 422
XapI RAATTY 1 cut(s) 49
XbaI TCTAGA 1 cut(s) 568
XceI RCATGY 1 cut(s) 340
XmnI GAANNNNTTC 1 cut(s) 170
XspI CTAG 1 cut(s) 569
ZraI GACGTC 1 cut(s) 559
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.