Rw4G006560

Retrotransposon gag protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0000370
Physical Location & Seq
Reverse (-)
13651623 .. 13652967
1345 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G006560.1

Sequence Viewer

Length: 1128 bp
ATGGATCTTCAGAGAGGCAGTGTTAAAGGCAGAGGCAGGTTTCCCCCTCAGAAGGAGTTTTATCAGGAAGATGAAGTGGAAGCACCGCTTTTGGTGCAGCCTGTTTTGCCTGTTTTTGAGGGCATCAATACTACTCGCCTATCTTGGGTGACTGAGGAAGTTACGAGATTGGGGGCAATCACATTTCCTGGTGGTACAGATTATATGCTAGCCGAATGCTGGATCGAGAACATGGAGACCTATTTTAAAATGATTATCTGTACTGACGTTGAGAAGTGGATAGTAGCTACATTTCTCCTCCAGGATGAGGCAAAACAATGGTGGAATTTTGTACTGAAGACAAAGGATATAGCCACCTTGACCTGGAAATGTTTTGTGGGACTCTTTCGAGATAAGTATTTTCCGGCTTCAGCGAGGGAGCAGATAGAGTTAGAGTTCATTGCTCTAACACAGGGGGCCATGACGGTGAGAGAATATGAGACACGGTTCACCCAGCTATACAGGTTCGTTCCGCAGATGGATGCTCGAGCCTTGGCTAAGAAGTTCTTAAGGGGACTAAATCACAGGATTAGGGAAATATTGCATCCCTTTCGGTTGGCTACCAAAGCAGACATCTTTGCTAGTGCCATGGCCCATGAACAAGCGGTCAACATTTGTGCAAGTGAACAGGGGGCTGTGAGGGAATCCCTCGGAAAGGGGAAAGCGATTGTAGGGAGCAGCAGTTCCAGGGATCATGGAAGTGGGTCATGGAAGCGACAGCGAACTTATTTCCATCCACAGGCCCCAGCCAGGATAGGTTATCAGCACCAGGCCCCAGCTAGGGCAGCATCGGCCCTATTTAAGGCTTTACCTGTGAGACAGGCAGCACCAGCTGCACCAACTGCACCAACTGCACCTGTGAGGTGCTATAACTGTGGTGAGCATGGCCACATCTTACGTGCGTGCACGAAACCGCGTAGCAGAGTATGCTACAAATGTAAGGTGAAAGGGCACTTTGCCAGGGAGTGTACCCGACCTCAGGGTAGGGTACAGGGAAACCAGAAGAGGCTACTACCATCGGCACCGGCTAGAGTCTTCGTTATTGGCCAGAGAGGCACTGAGGCGGAAGGAAATGATGGAGAAGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVP_2 Retrotrans_gag zf-CCHC

Protein Analysis

375

Amino Acids

42.28

Weight (kDa)

9.49

Isoelectric Point (pI)

48.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ty3_capsid PF19259 59 - 196 5.5e-08 Ty3 transposon capsid-like protein
Retrotrans_gag PF03732 95 - 188 2.4e-19 Retrotransposon gag protein
zf-CCHC PF00098 321 - 337 8.1e-06 Zinc knuckle
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000370)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g29481 FvH4_2g11454 FvH4_3g19661 FvH4_3g28463 FvH4_3g31691 FvH4_3g34551 FvH4_3g35241 FvH4_4g06575 FvH4_4g09082 FvH4_4g15804 FvH4_4g15805 FvH4_4g16178 FvH4_4g16282 FvH4_5g25163 FvH4_6g27413 FvH4_7g02962 FvH4_7g04361
malus_domestica MD01G1095000.v1.1 MD02G1270200.v1.1 MD02G1270300.v1.1 MD02G1270400.v1.1 MD06G1059100.v1.1 MD08G1226500.v1.1
prunus_persica Prupe.1G169500_v2.0.a1 Prupe.5G195800_v2.0.a1
pyrus_communis pycom01g00010 pycom01g00770 pycom02g23140 pycom03g11760 pycom04g06090 pycom05g06900 pycom05g08070 pycom05g14500 pycom06g03050 pycom07g06250 pycom07g07540 pycom07g10580 pycom08g04990 pycom1146g00060 pycom11g01080 pycom11g15270 pycom11g15460 pycom11g24900 pycom12419g00150 pycom12424g00120 pycom13g08740 pycom13g24950 pycom13g25960 pycom13g26030 pycom13g27820 pycom16g23270 pycom16g25020 pycom16g25960 pycom17g07780 pycom17g08210 pycom17g13100
rosa_chinensis RchiOBHm_Chr1g0329421 RchiOBHm_Chr4g0397711 RchiOBHm_Chr4g0403481 RchiOBHm_Chr5g0019091 RchiOBHm_Chr5g0049101
rosa_laevigata RLG00000029892
rosa_multiflora Rmu_sc0001866.1_g000007
rosa_roxburghii Rroxscaffold_2G00094160 Rroxscaffold_3G00229110 Rroxscaffold_4G00321100 Rroxscaffold_7G00196040 Rroxscaffold_7G00196280
rosa_rugosa Rorug05G0160500 Rorug06G0096500
rosa_samantha Rh1AG098600 Rh1AG098800 Rh1BG078200 Rh1BG078700 Rh1BG178500 Rh1CG094600 Rh1CG094900 Rh1DG101700 Rh1DG109600 Rh2AG175800 Rh2AG175900 Rh2AG511400 Rh2BG114700 Rh2CG042200 Rh2CG116600 Rh2CG317500 Rh2DG327700 Rh2DG337800 Rh4CG142700 Rh4DG113100 Rh5BG335800 Rh5CG360600 Rh5DG352000 Rh6BG071400 Rh6BG417100 Rh6CG463300 Rh6CG463400 Rh7BG186600 Rh7CG196500 Rh7CG196600 Rh7CG448900 Rh7DG228300
rosa_wichuraiana Rw4G006560 Rw4G017340 Rw5G030450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 27
AccB1I GGYRCC 1 cut(s) 1060
AccII CGCG 1 cut(s) 955
AciI CCGC 5 cut(s) 86, 512, 644, 953, 1103
AclWI GGATC 3 cut(s) 12, 230, 738
AcoI YGGCCR 2 cut(s) 925, 1084
AcsI RAATTY 1 cut(s) 325
AcuI CTGAAG 2 cut(s) 356, 393
AfaI GTAC 5 cut(s) 196, 262, 333, 1009, 1029
AflII CTTAAG 1 cut(s) 547
AjnI CCWGG 7 cut(s) 187, 300, 362, 725, 788, 807, 998
AloI GAACNNNNNNTCC 2 cut(s) 706, 738
AluBI AGCT 4 cut(s) 287, 496, 818, 872
AluI AGCT 4 cut(s) 287, 496, 818, 872
Alw21I GWGCWC 1 cut(s) 947
Alw26I GTCTC 3 cut(s) 230, 473, 850
Alw44I GTGCAC 1 cut(s) 943
AlwI GGATC 3 cut(s) 12, 230, 738
Ama87I CYCGRG 1 cut(s) 525
AoxI GGCC 7 cut(s) 456, 630, 780, 810, 831, 925, 1084
ApaLI GTGCAC 1 cut(s) 943
ApeKI GCWGC 5 cut(s) 97, 717, 824, 863, 872
ApoI RAATTY 1 cut(s) 325
AspS9I GGNCC 5 cut(s) 456, 631, 781, 811, 832
AsuHPI GGTGA 5 cut(s) 160, 478, 481, 929, 994
AsuNHI GCTAGC 1 cut(s) 208
AvaI CYCGRG 1 cut(s) 525
AxyI CCTNAGG 1 cut(s) 1017
BaeGI GKGCMC 2 cut(s) 947, 993
BalI TGGCCA 2 cut(s) 927, 1086
BanI GGYRCC 1 cut(s) 1060
BbsI GAAGAC 2 cut(s) 344, 1066
Bbv12I GWGCWC 1 cut(s) 947
BbvI GCAGC 5 cut(s) 109, 729, 836, 859, 875
BccI CCATC 4 cut(s) 511, 780, 1063, 1109
BcgI CGANNNNNNTGC 2 cut(s) 572, 606
BciT130I CCWGG 7 cut(s) 189, 302, 364, 727, 790, 809, 1000
BcoDI GTCTC 3 cut(s) 230, 473, 850
BfaI CTAG 4 cut(s) 209, 621, 819, 1068
BfrI CTTAAG 1 cut(s) 547
BfuAI ACCTGC 1 cut(s) 27
BglI GCCNNNNNGGC 1 cut(s) 1092
BisI GCNGC 5 cut(s) 98, 718, 825, 864, 873
BlsI GCNGC 5 cut(s) 99, 719, 826, 865, 874
Bme1390I CCNGG 7 cut(s) 189, 302, 364, 727, 790, 809, 1000
BmeT110I CYCGRG 1 cut(s) 525
BmgT120I GGNCC 5 cut(s) 456, 631, 781, 811, 832
BmiI GGNNCC 4 cut(s) 457, 783, 813, 1062
BmrFI CCNGG 7 cut(s) 189, 302, 364, 727, 790, 809, 1000
BmsI GCATC 4 cut(s) 132, 511, 592, 836
BmtI GCTAGC 1 cut(s) 212
BpiI GAAGAC 2 cut(s) 344, 1066
BpmI CTGGAG 1 cut(s) 284
BsaAI YACGTR 1 cut(s) 938
BsaI GGTCTC 1 cut(s) 230
BsaJI CCNNGG 5 cut(s) 531, 627, 688, 726, 999
BsaXI ACNNNNNCTCC 2 cut(s) 706, 736
Bse118I RCCGGY 1 cut(s) 1063
Bse21I CCTNAGG 1 cut(s) 1017
Bse3DI GCAATG 1 cut(s) 438
BseBI CCWGG 7 cut(s) 189, 302, 364, 727, 790, 809, 1000
BseDI CCNNGG 5 cut(s) 531, 627, 688, 726, 999
BseGI GGATG 4 cut(s) 310, 526, 583, 772
BseMI GCAATG 1 cut(s) 438
BseMII CTCAG 4 cut(s) 62, 144, 1031, 1089
BseRI GAGGAG 1 cut(s) 287
BseSI GKGCMC 2 cut(s) 947, 993
BseXI GCAGC 5 cut(s) 109, 729, 836, 859, 875
BseYI CCCAGC 3 cut(s) 492, 784, 814
BsgI GTGCAG 4 cut(s) 116, 858, 867, 876
Bsh1236I CGCG 1 cut(s) 955
BshFI GGCC 7 cut(s) 458, 632, 782, 812, 833, 927, 1086
BshNI GGYRCC 1 cut(s) 1060
BsiHKAI GWGCWC 1 cut(s) 947
BsiHKCI CYCGRG 1 cut(s) 525
BsiSI CCGG 2 cut(s) 404, 1064
BslFI GGGAC 2 cut(s) 393, 567
BsmAI GTCTC 3 cut(s) 230, 473, 850
BsmFI GGGAC 2 cut(s) 393, 567
BsmI GAATGC 1 cut(s) 221
BsnI GGCC 7 cut(s) 458, 632, 782, 812, 833, 927, 1086
Bso31I GGTCTC 1 cut(s) 230
BsoBI CYCGRG 1 cut(s) 525
Bsp1286I GDGCHC 2 cut(s) 947, 993
Bsp143I GATC 3 cut(s) 4, 222, 730
Bsp19I CCATGG 1 cut(s) 627
BspACI CCGC 5 cut(s) 86, 512, 644, 953, 1103
BspANI GGCC 7 cut(s) 458, 632, 782, 812, 833, 927, 1086
BspCNI CTCAG 4 cut(s) 61, 145, 1030, 1090
BspFNI CGCG 1 cut(s) 955
BspLI GGNNCC 4 cut(s) 457, 783, 813, 1062
BspMI ACCTGC 1 cut(s) 27
BspOI GCTAGC 1 cut(s) 212
BspPI GGATC 3 cut(s) 12, 230, 738
BspT107I GGYRCC 1 cut(s) 1060
BspTI CTTAAG 1 cut(s) 547
BspTNI GGTCTC 1 cut(s) 230
BsrDI GCAATG 1 cut(s) 438
BsrFI RCCGGY 1 cut(s) 1063
BssAI RCCGGY 1 cut(s) 1063
BssECI CCNNGG 5 cut(s) 531, 627, 688, 726, 999
BssMI GATC 3 cut(s) 4, 222, 730
BssT1I CCWWGG 2 cut(s) 531, 627
Bst2UI CCWGG 7 cut(s) 189, 302, 364, 727, 790, 809, 1000
Bst4CI ACNGT 3 cut(s) 466, 486, 914
Bst6I CTCTTC 1 cut(s) 1037
BstAFI CTTAAG 1 cut(s) 547
BstAPI GCANNNNNTGC 4 cut(s) 872, 881, 890, 966
BstBAI YACGTR 1 cut(s) 938
BstC8I GCNNGC 2 cut(s) 210, 943
BstDEI CTNAG 5 cut(s) 48, 153, 537, 1017, 1098
BstDSI CCRYGG 1 cut(s) 627
BstENI CCTNNNNNAGG 2 cut(s) 692, 839
BstF5I GGATG 4 cut(s) 310, 526, 583, 772
BstFNI CGCG 1 cut(s) 955
BstKTI GATC 3 cut(s) 7, 225, 733
BstMAI GTCTC 3 cut(s) 230, 473, 850
BstMBI GATC 3 cut(s) 4, 222, 730
BstNI CCWGG 7 cut(s) 189, 302, 364, 727, 790, 809, 1000
BstSCI CCNGG 7 cut(s) 187, 300, 362, 725, 788, 807, 998
BstSLI GKGCMC 2 cut(s) 947, 993
BstUI CGCG 1 cut(s) 955
BstV1I GCAGC 5 cut(s) 109, 729, 836, 859, 875
BstV2I GAAGAC 2 cut(s) 344, 1066
BstX2I RGATCY 1 cut(s) 4
BstYI RGATCY 1 cut(s) 4
Bsu36I CCTNAGG 1 cut(s) 1017
BsuRI GGCC 7 cut(s) 458, 632, 782, 812, 833, 927, 1086
BtgI CCRYGG 1 cut(s) 627
BtsCI GGATG 4 cut(s) 310, 526, 583, 772
BtsI GCAGTG 1 cut(s) 25
BtsIMutI CAGTG 2 cut(s) 25, 1095
BveI ACCTGC 1 cut(s) 27
Cac8I GCNNGC 2 cut(s) 210, 943
Cfr10I RCCGGY 1 cut(s) 1063
Cfr13I GGNCC 5 cut(s) 456, 631, 781, 811, 832
Csp6I GTAC 5 cut(s) 195, 261, 332, 1008, 1028
CviAII CATG 8 cut(s) 232, 460, 628, 635, 734, 747, 923, 1125
CviQI GTAC 5 cut(s) 195, 261, 332, 1008, 1028
DdeI CTNAG 5 cut(s) 48, 153, 537, 1017, 1098
DpnI GATC 3 cut(s) 6, 224, 732
DpnII GATC 3 cut(s) 4, 222, 730
DraI TTTAAA 1 cut(s) 247
EaeI YGGCCR 2 cut(s) 925, 1084
Eam1104I CTCTTC 1 cut(s) 1037
EarI CTCTTC 1 cut(s) 1037
EciI GGCGGA 1 cut(s) 1118
Eco130I CCWWGG 2 cut(s) 531, 627
Eco31I GGTCTC 1 cut(s) 230
Eco57I CTGAAG 2 cut(s) 356, 393
Eco81I CCTNAGG 1 cut(s) 1017
Eco88I CYCGRG 1 cut(s) 525
EcoNI CCTNNNNNAGG 2 cut(s) 692, 839
EcoO109I RGGNCCY 2 cut(s) 781, 811
EcoRII CCWGG 7 cut(s) 187, 300, 362, 725, 788, 807, 998
EcoT14I CCWWGG 2 cut(s) 531, 627
ErhI CCWWGG 2 cut(s) 531, 627
FaeI CATG 8 cut(s) 235, 463, 631, 638, 737, 750, 926, 1128
FalI AAGNNNNNCTT 4 cut(s) 72, 104, 530, 562
FaqI GGGAC 2 cut(s) 393, 567
FatI CATG 8 cut(s) 231, 459, 627, 634, 733, 746, 922, 1124
Fnu4HI GCNGC 5 cut(s) 98, 718, 825, 864, 873
FokI GGATG 4 cut(s) 317, 533, 570, 759
Fsp4HI GCNGC 5 cut(s) 98, 718, 825, 864, 873
FspBI CTAG 4 cut(s) 209, 621, 819, 1068
GluI GCNGC 5 cut(s) 98, 718, 825, 864, 873
GsaI CCCAGC 3 cut(s) 496, 788, 818
GsuI CTGGAG 1 cut(s) 284
HaeIII GGCC 7 cut(s) 458, 632, 782, 812, 833, 927, 1086
HapII CCGG 2 cut(s) 404, 1064
Hin1II CATG 8 cut(s) 235, 463, 631, 638, 737, 750, 926, 1128
HincII GTYRAC 1 cut(s) 649
HindII GTYRAC 1 cut(s) 649
HinfI GANTC 3 cut(s) 381, 683, 1071
HpaII CCGG 2 cut(s) 404, 1064
HphI GGTGA 5 cut(s) 160, 478, 481, 929, 994
Hpy166II GTNNAC 5 cut(s) 489, 649, 665, 945, 1008
Hpy188I TCNGA 3 cut(s) 12, 51, 692
Hpy188III TCNNGA 3 cut(s) 65, 226, 389
Hpy8I GTNNAC 5 cut(s) 489, 649, 665, 945, 1008
HpyAV CCTTC 2 cut(s) 46, 1100
HpyCH4III ACNGT 3 cut(s) 466, 486, 914
HpyCH4IV ACGT 2 cut(s) 267, 937
HpyCH4V TGCA 7 cut(s) 97, 583, 659, 875, 884, 893, 945
HpyF3I CTNAG 5 cut(s) 48, 153, 537, 1017, 1098
HpySE526I ACGT 2 cut(s) 267, 937
Hsp92II CATG 8 cut(s) 235, 463, 631, 638, 737, 750, 926, 1128
Kzo9I GATC 3 cut(s) 4, 222, 730
LmnI GCTCC 2 cut(s) 418, 714
Lsp1109I GCAGC 5 cut(s) 109, 729, 836, 859, 875
LweI GCATC 4 cut(s) 132, 511, 592, 836
MaeI CTAG 4 cut(s) 209, 621, 819, 1068
MaeII ACGT 2 cut(s) 267, 937
MaeIII GTNAC 2 cut(s) 148, 160
MalI GATC 3 cut(s) 6, 224, 732
MboI GATC 3 cut(s) 4, 222, 730
MboII GAAGA 4 cut(s) 80, 349, 1054, 1066
MflI RGATCY 1 cut(s) 4
MhlI GDGCHC 2 cut(s) 947, 993
MlsI TGGCCA 2 cut(s) 927, 1086
MluCI AATT 1 cut(s) 325
MluNI TGGCCA 2 cut(s) 927, 1086
MlyI GAGTC 2 cut(s) 375, 1080
Mox20I TGGCCA 2 cut(s) 927, 1086
MscI TGGCCA 2 cut(s) 927, 1086
MseI TTAA 4 cut(s) 24, 246, 548, 840
MslI CAYNNNNRTG 2 cut(s) 464, 738
Msp20I TGGCCA 2 cut(s) 927, 1086
MspA1I CMGCKG 1 cut(s) 872
MspCI CTTAAG 1 cut(s) 547
MspI CCGG 2 cut(s) 404, 1064
MspR9I CCNGG 7 cut(s) 189, 302, 364, 727, 790, 809, 1000
Mva1269I GAATGC 1 cut(s) 221
MvaI CCWGG 7 cut(s) 189, 302, 364, 727, 790, 809, 1000
MvnI CGCG 1 cut(s) 955
NcoI CCATGG 1 cut(s) 627
NdeII GATC 3 cut(s) 4, 222, 730
NheI GCTAGC 1 cut(s) 208
NlaIII CATG 8 cut(s) 235, 463, 631, 638, 737, 750, 926, 1128
NlaIV GGNNCC 4 cut(s) 457, 783, 813, 1062
NmuCI GTSAC 1 cut(s) 148
PaeR7I CTCGAG 1 cut(s) 525
PctI GAATGC 1 cut(s) 221
PfeI GAWTC 1 cut(s) 683
PfoI TCCNGGA 1 cut(s) 300
PkrI GCNGC 5 cut(s) 99, 719, 826, 865, 874
PleI GAGTC 2 cut(s) 375, 1079
PpsI GAGTC 2 cut(s) 375, 1079
Ppu21I YACGTR 1 cut(s) 938
Psp6I CCWGG 7 cut(s) 187, 300, 362, 725, 788, 807, 998
PspFI CCCAGC 3 cut(s) 492, 784, 814
PspGI CCWGG 7 cut(s) 187, 300, 362, 725, 788, 807, 998
PspN4I GGNNCC 4 cut(s) 457, 783, 813, 1062
PspPI GGNCC 5 cut(s) 456, 631, 781, 811, 832
PspXI VCTCGAGB 1 cut(s) 525
PsuI RGATCY 1 cut(s) 4
PvuII CAGCTG 1 cut(s) 872
RsaI GTAC 5 cut(s) 196, 262, 333, 1009, 1029
RsaNI GTAC 5 cut(s) 195, 261, 332, 1008, 1028
RseI CAYNNNNRTG 2 cut(s) 464, 738
SaqAI TTAA 4 cut(s) 24, 246, 548, 840
SatI GCNGC 5 cut(s) 98, 718, 825, 864, 873
Sau3AI GATC 3 cut(s) 4, 222, 730
Sau96I GGNCC 5 cut(s) 456, 631, 781, 811, 832
SchI GAGTC 2 cut(s) 375, 1080
ScrFI CCNGG 7 cut(s) 189, 302, 364, 727, 790, 809, 1000
SduI GDGCHC 2 cut(s) 947, 993
SfaNI GCATC 4 cut(s) 132, 511, 592, 836
Sfr274I CTCGAG 1 cut(s) 525
SlaI CTCGAG 1 cut(s) 525
SmiMI CAYNNNNRTG 2 cut(s) 464, 738
SmlI CTYRAG 2 cut(s) 525, 547
SmoI CTYRAG 2 cut(s) 525, 547
Sse9I AATT 1 cut(s) 325
SsiI CCGC 5 cut(s) 86, 512, 644, 953, 1103
SspI AATATT 1 cut(s) 579
SspMI CTAG 4 cut(s) 209, 621, 819, 1068
StyD4I CCNGG 7 cut(s) 187, 300, 362, 725, 788, 807, 998
StyI CCWWGG 2 cut(s) 531, 627
TaaI ACNGT 3 cut(s) 466, 486, 914
TaiI ACGT 2 cut(s) 270, 940
TaqI TCGA 3 cut(s) 225, 388, 526
TasI AATT 1 cut(s) 325
TatI WGTACW 2 cut(s) 260, 331
TfiI GAWTC 1 cut(s) 683
Tru1I TTAA 4 cut(s) 24, 246, 548, 840
Tru9I TTAA 4 cut(s) 24, 246, 548, 840
TscAI CASTG 2 cut(s) 25, 1102
TseFI GTSAC 1 cut(s) 148
TseI GCWGC 5 cut(s) 97, 717, 824, 863, 872
Tsp45I GTSAC 1 cut(s) 148
TspDTI ATGAA 3 cut(s) 87, 427, 651
TspRI CASTG 2 cut(s) 25, 1102
Vha464I CTTAAG 1 cut(s) 547
VneI GTGCAC 1 cut(s) 943
XagI CCTNNNNNAGG 2 cut(s) 692, 839
XapI RAATTY 1 cut(s) 325
XhoI CTCGAG 1 cut(s) 525
XspI CTAG 4 cut(s) 209, 621, 819, 1068
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.