protease
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccBSI | CCGCTC | 2 cut(s) | 108, 282 |
| AccI | GTMKAC | 1 cut(s) | 945 |
| AccII | CGCG | 7 cut(s) | 57, 177, 311, 366, 402, 808, 1755 |
| AclWI | GGATC | 3 cut(s) | 485, 986, 1557 |
| AcoI | YGGCCR | 1 cut(s) | 403 |
| AcsI | RAATTY | 6 cut(s) | 459, 469, 498, 596, 925, 1848 |
| AcuI | CTGAAG | 4 cut(s) | 443, 1040, 1552, 1560 |
| AcyI | GRCGYC | 1 cut(s) | 1413 |
| AfaI | GTAC | 4 cut(s) | 488, 1038, 1167, 1231 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 357, 623, 1948 |
| AgeI | ACCGGT | 1 cut(s) | 1700 |
| AgsI | TTSAA | 7 cut(s) | 524, 532, 583, 964, 1014, 1507, 2011 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 190 |
| AjnI | CCWGG | 4 cut(s) | 405, 1186, 1282, 1476 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1982, 2014 |
| Alw21I | GWGCWC | 1 cut(s) | 1821 |
| Alw26I | GTCTC | 3 cut(s) | 194, 1623, 1960 |
| AlwI | GGATC | 3 cut(s) | 485, 986, 1557 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 1969 |
| AoxI | GGCC | 4 cut(s) | 403, 606, 1257, 1986 |
| ApoI | RAATTY | 6 cut(s) | 459, 469, 498, 596, 925, 1848 |
| AseI | ATTAAT | 1 cut(s) | 933 |
| AsiGI | ACCGGT | 1 cut(s) | 1700 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 320 |
| AspLEI | GCGC | 8 cut(s) | 59, 179, 311, 366, 402, 808, 810, 1757 |
| AspS9I | GGNCC | 1 cut(s) | 1698 |
| AsuC2I | CCSGG | 2 cut(s) | 504, 1942 |
| AsuHPI | GGTGA | 4 cut(s) | 31, 697, 806, 1301 |
| AsuII | TTCGAA | 1 cut(s) | 1270 |
| AvaII | GGWCC | 1 cut(s) | 1698 |
| BanII | GRGCYC | 2 cut(s) | 705, 1821 |
| BauI | CACGAG | 1 cut(s) | 1811 |
| BbsI | GAAGAC | 2 cut(s) | 571, 1175 |
| Bbv12I | GWGCWC | 1 cut(s) | 1821 |
| BccI | CCATC | 5 cut(s) | 140, 224, 398, 1939, 2003 |
| BceAI | ACGGC | 1 cut(s) | 53 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 52, 86 |
| BciT130I | CCWGG | 4 cut(s) | 407, 1188, 1284, 1478 |
| BciVI | GTATCC | 1 cut(s) | 1382 |
| BclI | TGATCA | 2 cut(s) | 415, 796 |
| BcnI | CCSGG | 2 cut(s) | 504, 1942 |
| BcoDI | GTCTC | 3 cut(s) | 194, 1623, 1960 |
| BfaI | CTAG | 3 cut(s) | 648, 1194, 1869 |
| BfmI | CTRYAG | 2 cut(s) | 1659, 1907 |
| BfuI | GTATCC | 1 cut(s) | 1382 |
| Bme1390I | CCNGG | 6 cut(s) | 407, 504, 1188, 1284, 1478, 1942 |
| Bme18I | GGWCC | 1 cut(s) | 1698 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 190 |
| BmgT120I | GGNCC | 1 cut(s) | 1698 |
| BmiI | GGNNCC | 2 cut(s) | 1447, 2026 |
| BmrFI | CCNGG | 6 cut(s) | 407, 504, 1188, 1284, 1478, 1942 |
| BmsI | GCATC | 7 cut(s) | 828, 1154, 1282, 1349, 1367, 1699, 1723 |
| BpiI | GAAGAC | 2 cut(s) | 571, 1175 |
| BpmI | CTGGAG | 3 cut(s) | 110, 1305, 1991 |
| Bpu14I | TTCGAA | 1 cut(s) | 1270 |
| BpuEI | CTTGAG | 2 cut(s) | 758, 1760 |
| BpuMI | CCSGG | 2 cut(s) | 504, 1942 |
| Bsa29I | ATCGAT | 1 cut(s) | 1652 |
| BsaAI | YACGTR | 1 cut(s) | 1229 |
| BsaHI | GRCGYC | 1 cut(s) | 1413 |
| BsaI | GGTCTC | 1 cut(s) | 194 |
| BsaJI | CCNNGG | 5 cut(s) | 204, 267, 356, 1282, 1477 |
| BsaWI | WCCGGW | 1 cut(s) | 1700 |
| BsaXI | ACNNNNNCTCC | 3 cut(s) | 1904, 1934, 2009 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 357, 623, 1948 |
| Bse118I | RCCGGY | 2 cut(s) | 791, 1700 |
| Bse1I | ACTGG | 3 cut(s) | 127, 438, 1974 |
| Bse3DI | GCAATG | 6 cut(s) | 345, 848, 1100, 1353, 1407, 1899 |
| BseBI | CCWGG | 4 cut(s) | 407, 1188, 1284, 1478 |
| BseCI | ATCGAT | 1 cut(s) | 1652 |
| BseDI | CCNNGG | 5 cut(s) | 204, 267, 356, 1282, 1477 |
| BseGI | GGATG | 3 cut(s) | 12, 886, 1950 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 357, 623, 1948 |
| BseMI | GCAATG | 6 cut(s) | 345, 848, 1100, 1353, 1407, 1899 |
| BseMII | CTCAG | 1 cut(s) | 1997 |
| BseNI | ACTGG | 3 cut(s) | 127, 438, 1974 |
| BsePI | GCGCGC | 1 cut(s) | 806 |
| BseRI | GAGGAG | 3 cut(s) | 8, 88, 1688 |
| BsgI | GTGCAG | 1 cut(s) | 1587 |
| Bsh1236I | CGCG | 7 cut(s) | 57, 177, 311, 366, 402, 808, 1755 |
| BshFI | GGCC | 4 cut(s) | 405, 608, 1259, 1988 |
| BshTI | ACCGGT | 1 cut(s) | 1700 |
| BshVI | ATCGAT | 1 cut(s) | 1652 |
| BsiHKAI | GWGCWC | 1 cut(s) | 1821 |
| BsiSI | CCGG | 6 cut(s) | 197, 504, 792, 1701, 1926, 1942 |
| BsiWI | CGTACG | 1 cut(s) | 486 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 357, 623, 1948 |
| BsmAI | GTCTC | 3 cut(s) | 194, 1623, 1960 |
| BsmI | GAATGC | 1 cut(s) | 1615 |
| BsnI | GGCC | 4 cut(s) | 405, 608, 1259, 1988 |
| Bso31I | GGTCTC | 1 cut(s) | 194 |
| Bsp119I | TTCGAA | 1 cut(s) | 1270 |
| Bsp1286I | GDGCHC | 2 cut(s) | 705, 1821 |
| Bsp1407I | TGTACA | 1 cut(s) | 1165 |
| Bsp143I | GATC | 6 cut(s) | 415, 490, 796, 978, 1562, 1649 |
| BspANI | GGCC | 4 cut(s) | 405, 608, 1259, 1988 |
| BspCNI | CTCAG | 1 cut(s) | 1996 |
| BspDI | ATCGAT | 1 cut(s) | 1652 |
| BspFNI | CGCG | 7 cut(s) | 57, 177, 311, 366, 402, 808, 1755 |
| BspLI | GGNNCC | 2 cut(s) | 1447, 2026 |
| BspMAI | CTGCAG | 1 cut(s) | 1911 |
| BspPI | GGATC | 3 cut(s) | 485, 986, 1557 |
| BspQI | GCTCTTC | 1 cut(s) | 311 |
| BspT104I | TTCGAA | 1 cut(s) | 1270 |
| BspTNI | GGTCTC | 1 cut(s) | 194 |
| BsrBI | CCGCTC | 2 cut(s) | 108, 282 |
| BsrDI | GCAATG | 6 cut(s) | 345, 848, 1100, 1353, 1407, 1899 |
| BsrFI | RCCGGY | 2 cut(s) | 791, 1700 |
| BsrGI | TGTACA | 1 cut(s) | 1165 |
| BsrI | ACTGG | 3 cut(s) | 127, 438, 1974 |
| BssAI | RCCGGY | 2 cut(s) | 791, 1700 |
| BssECI | CCNNGG | 5 cut(s) | 204, 267, 356, 1282, 1477 |
| BssHII | GCGCGC | 1 cut(s) | 806 |
| BssMI | GATC | 6 cut(s) | 415, 490, 796, 978, 1562, 1649 |
| BssNI | GRCGYC | 1 cut(s) | 1413 |
| BssSI | CACGAG | 1 cut(s) | 1811 |
| BssT1I | CCWWGG | 3 cut(s) | 204, 267, 356 |
| Bst2BI | CACGAG | 1 cut(s) | 1811 |
| Bst2UI | CCWGG | 4 cut(s) | 407, 1188, 1284, 1478 |
| Bst4CI | ACNGT | 6 cut(s) | 709, 1102, 1212, 1234, 1488, 1660 |
| Bst6I | CTCTTC | 5 cut(s) | 92, 311, 419, 819, 1059 |
| BstACI | GRCGYC | 1 cut(s) | 1413 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1610 |
| BstAUI | TGTACA | 1 cut(s) | 1165 |
| BstBAI | YACGTR | 1 cut(s) | 1229 |
| BstBI | TTCGAA | 1 cut(s) | 1270 |
| BstC8I | GCNNGC | 8 cut(s) | 61, 175, 307, 341, 622, 655, 808, 1821 |
| BstDEI | CTNAG | 2 cut(s) | 1596, 1983 |
| BstF5I | GGATG | 3 cut(s) | 12, 886, 1950 |
| BstFNI | CGCG | 7 cut(s) | 57, 177, 311, 366, 402, 808, 1755 |
| BstHHI | GCGC | 8 cut(s) | 59, 179, 311, 366, 402, 808, 810, 1757 |
| BstKTI | GATC | 6 cut(s) | 418, 493, 799, 981, 1565, 1652 |
| BstMAI | GTCTC | 3 cut(s) | 194, 1623, 1960 |
| BstMBI | GATC | 6 cut(s) | 415, 490, 796, 978, 1562, 1649 |
| BstNI | CCWGG | 4 cut(s) | 407, 1188, 1284, 1478 |
| BstNSI | RCATGY | 1 cut(s) | 343 |
| BstSCI | CCNGG | 6 cut(s) | 405, 502, 1186, 1282, 1476, 1940 |
| BstSFI | CTRYAG | 2 cut(s) | 1659, 1907 |
| BstSNI | TACGTA | 1 cut(s) | 1229 |
| BstUI | CGCG | 7 cut(s) | 57, 177, 311, 366, 402, 808, 1755 |
| BstV2I | GAAGAC | 2 cut(s) | 571, 1175 |
| Bsu15I | ATCGAT | 1 cut(s) | 1652 |
| BsuI | GTATCC | 1 cut(s) | 1382 |
| BsuRI | GGCC | 4 cut(s) | 405, 608, 1259, 1988 |
| BsuTUI | ATCGAT | 1 cut(s) | 1652 |
| BtgZI | GCGATG | 2 cut(s) | 243, 249 |
| BtsCI | GGATG | 3 cut(s) | 12, 886, 1950 |
| BtsI | GCAGTG | 1 cut(s) | 1838 |
| BtsIMutI | CAGTG | 2 cut(s) | 445, 1838 |
| Cac8I | GCNNGC | 8 cut(s) | 61, 175, 307, 341, 622, 655, 808, 1821 |
| CaiI | CAGNNNCTG | 1 cut(s) | 1969 |
| CfoI | GCGC | 8 cut(s) | 59, 179, 311, 366, 402, 808, 810, 1757 |
| Cfr10I | RCCGGY | 2 cut(s) | 791, 1700 |
| Cfr13I | GGNCC | 1 cut(s) | 1698 |
| ClaI | ATCGAT | 1 cut(s) | 1652 |
| CseI | GACGC | 3 cut(s) | 355, 370, 1421 |
| Csp6I | GTAC | 4 cut(s) | 487, 1037, 1166, 1230 |
| CspAI | ACCGGT | 1 cut(s) | 1700 |
| CviAII | CATG | 4 cut(s) | 340, 817, 1396, 1957 |
| CviQI | GTAC | 4 cut(s) | 487, 1037, 1166, 1230 |
| DdeI | CTNAG | 2 cut(s) | 1596, 1983 |
| DpnI | GATC | 6 cut(s) | 417, 492, 798, 980, 1564, 1651 |
| DpnII | GATC | 6 cut(s) | 415, 490, 796, 978, 1562, 1649 |
| DriI | GACNNNNNGTC | 1 cut(s) | 190 |
| EaeI | YGGCCR | 1 cut(s) | 403 |
| Eam1104I | CTCTTC | 5 cut(s) | 92, 311, 419, 819, 1059 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 190 |
| EarI | CTCTTC | 5 cut(s) | 92, 311, 419, 819, 1059 |
| EciI | GGCGGA | 3 cut(s) | 110, 1758, 1944 |
| Ecl136II | GAGCTC | 1 cut(s) | 1819 |
| Eco105I | TACGTA | 1 cut(s) | 1229 |
| Eco130I | CCWWGG | 3 cut(s) | 204, 267, 356 |
| Eco24I | GRGCYC | 2 cut(s) | 705, 1821 |
| Eco31I | GGTCTC | 1 cut(s) | 194 |
| Eco32I | GATATC | 1 cut(s) | 919 |
| Eco47I | GGWCC | 1 cut(s) | 1698 |
| Eco53kI | GAGCTC | 1 cut(s) | 1819 |
| Eco57I | CTGAAG | 4 cut(s) | 443, 1040, 1552, 1560 |
| EcoICRI | GAGCTC | 1 cut(s) | 1819 |
| EcoRI | GAATTC | 1 cut(s) | 498 |
| EcoRII | CCWGG | 4 cut(s) | 405, 1186, 1282, 1476 |
| EcoRV | GATATC | 1 cut(s) | 919 |
| EcoT14I | CCWWGG | 3 cut(s) | 204, 267, 356 |
| EcoT22I | ATGCAT | 1 cut(s) | 1615 |
| EcoT38I | GRGCYC | 2 cut(s) | 705, 1821 |
| ErhI | CCWWGG | 3 cut(s) | 204, 267, 356 |
| FaeI | CATG | 4 cut(s) | 343, 820, 1399, 1960 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1487, 1519 |
| FatI | CATG | 4 cut(s) | 339, 816, 1395, 1956 |
| FauI | CCCGC | 1 cut(s) | 625 |
| FbaI | TGATCA | 2 cut(s) | 415, 796 |
| FblI | GTMKAC | 1 cut(s) | 945 |
| FokI | GGATG | 2 cut(s) | 893, 1957 |
| FriOI | GRGCYC | 2 cut(s) | 705, 1821 |
| FspBI | CTAG | 3 cut(s) | 648, 1194, 1869 |
| GlaI | GCGC | 8 cut(s) | 58, 178, 310, 365, 401, 807, 809, 1756 |
| GsuI | CTGGAG | 3 cut(s) | 110, 1305, 1991 |
| HaeIII | GGCC | 4 cut(s) | 405, 608, 1259, 1988 |
| HapII | CCGG | 6 cut(s) | 197, 504, 792, 1701, 1926, 1942 |
| HgaI | GACGC | 3 cut(s) | 355, 370, 1421 |
| HhaI | GCGC | 8 cut(s) | 59, 179, 311, 366, 402, 808, 810, 1757 |
| Hin1I | GRCGYC | 1 cut(s) | 1413 |
| Hin1II | CATG | 4 cut(s) | 343, 820, 1399, 1960 |
| Hin6I | GCGC | 8 cut(s) | 57, 177, 309, 364, 400, 806, 808, 1755 |
| HinP1I | GCGC | 8 cut(s) | 57, 177, 309, 364, 400, 806, 808, 1755 |
| HincII | GTYRAC | 1 cut(s) | 1917 |
| HindII | GTYRAC | 1 cut(s) | 1917 |
| HinfI | GANTC | 8 cut(s) | 185, 215, 427, 750, 942, 1241, 1932, 1953 |
| HpaII | CCGG | 6 cut(s) | 197, 504, 792, 1701, 1926, 1942 |
| HphI | GGTGA | 4 cut(s) | 31, 697, 806, 1301 |
| Hpy166II | GTNNAC | 4 cut(s) | 443, 946, 1166, 1917 |
| Hpy188III | TCNNGA | 8 cut(s) | 298, 370, 385, 583, 737, 922, 1628, 1647 |
| Hpy8I | GTNNAC | 4 cut(s) | 443, 946, 1166, 1917 |
| Hpy99I | CGWCG | 4 cut(s) | 74, 193, 196, 329 |
| HpyAV | CCTTC | 9 cut(s) | 190, 214, 295, 361, 932, 965, 1015, 1576, 1891 |
| HpyCH4III | ACNGT | 6 cut(s) | 709, 1102, 1212, 1234, 1488, 1660 |
| HpyCH4IV | ACGT | 2 cut(s) | 1228, 1919 |
| HpyF3I | CTNAG | 2 cut(s) | 1596, 1983 |
| HpySE526I | ACGT | 2 cut(s) | 1228, 1919 |
| Hsp92I | GRCGYC | 1 cut(s) | 1413 |
| Hsp92II | CATG | 4 cut(s) | 343, 820, 1399, 1960 |
| HspAI | GCGC | 8 cut(s) | 57, 177, 309, 364, 400, 806, 808, 1755 |
| Ksp22I | TGATCA | 2 cut(s) | 415, 796 |
| Kzo9I | GATC | 6 cut(s) | 415, 490, 796, 978, 1562, 1649 |
| LguI | GCTCTTC | 1 cut(s) | 311 |
| LmnI | GCTCC | 7 cut(s) | 113, 162, 339, 1816, 1865, 1976, 2030 |
| LweI | GCATC | 7 cut(s) | 828, 1154, 1282, 1349, 1367, 1699, 1723 |
| MaeI | CTAG | 3 cut(s) | 648, 1194, 1869 |
| MaeII | ACGT | 2 cut(s) | 1228, 1919 |
| MaeIII | GTNAC | 2 cut(s) | 1459, 1703 |
| MalI | GATC | 6 cut(s) | 417, 492, 798, 980, 1564, 1651 |
| MbiI | CCGCTC | 2 cut(s) | 108, 282 |
| MboI | GATC | 6 cut(s) | 415, 490, 796, 978, 1562, 1649 |
| MhlI | GDGCHC | 2 cut(s) | 705, 1821 |
| MlyI | GAGTC | 4 cut(s) | 179, 436, 951, 1941 |
| MmeI | TCCRAC | 3 cut(s) | 727, 1084, 1858 |
| Mph1103I | ATGCAT | 1 cut(s) | 1615 |
| MroXI | GAANNNNTTC | 1 cut(s) | 320 |
| MseI | TTAA | 6 cut(s) | 246, 933, 1178, 1439, 1473, 1853 |
| MslI | CAYNNNNRTG | 2 cut(s) | 815, 1400 |
| MspA1I | CMGCKG | 2 cut(s) | 421, 1553 |
| MspI | CCGG | 6 cut(s) | 197, 504, 792, 1701, 1926, 1942 |
| MspR9I | CCNGG | 6 cut(s) | 407, 504, 1188, 1284, 1478, 1942 |
| Mva1269I | GAATGC | 1 cut(s) | 1615 |
| MvaI | CCWGG | 4 cut(s) | 407, 1188, 1284, 1478 |
| MvnI | CGCG | 7 cut(s) | 57, 177, 311, 366, 402, 808, 1755 |
| NciI | CCSGG | 2 cut(s) | 504, 1942 |
| NdeII | GATC | 6 cut(s) | 415, 490, 796, 978, 1562, 1649 |
| NlaIII | CATG | 4 cut(s) | 343, 820, 1399, 1960 |
| NlaIV | GGNNCC | 2 cut(s) | 1447, 2026 |
| NmuCI | GTSAC | 1 cut(s) | 1459 |
| NsiI | ATGCAT | 1 cut(s) | 1615 |
| NspI | RCATGY | 1 cut(s) | 343 |
| NspV | TTCGAA | 1 cut(s) | 1270 |
| PaeI | GCATGC | 1 cut(s) | 343 |
| PauI | GCGCGC | 1 cut(s) | 806 |
| PciSI | GCTCTTC | 1 cut(s) | 311 |
| PctI | GAATGC | 1 cut(s) | 1615 |
| PdmI | GAANNNNTTC | 1 cut(s) | 320 |
| PfeI | GAWTC | 4 cut(s) | 215, 750, 1241, 1953 |
| Pfl23II | CGTACG | 1 cut(s) | 486 |
| PinAI | ACCGGT | 1 cut(s) | 1700 |
| PleI | GAGTC | 4 cut(s) | 179, 435, 950, 1940 |
| PpsI | GAGTC | 4 cut(s) | 179, 435, 950, 1940 |
| Ppu21I | YACGTR | 1 cut(s) | 1229 |
| PshBI | ATTAAT | 1 cut(s) | 933 |
| Psp124BI | GAGCTC | 1 cut(s) | 1821 |
| Psp6I | CCWGG | 4 cut(s) | 405, 1186, 1282, 1476 |
| PspGI | CCWGG | 4 cut(s) | 405, 1186, 1282, 1476 |
| PspLI | CGTACG | 1 cut(s) | 486 |
| PspN4I | GGNNCC | 2 cut(s) | 1447, 2026 |
| PspPI | GGNCC | 1 cut(s) | 1698 |
| PstI | CTGCAG | 1 cut(s) | 1911 |
| PstNI | CAGNNNCTG | 1 cut(s) | 1969 |
| PteI | GCGCGC | 1 cut(s) | 806 |
| PvuII | CAGCTG | 2 cut(s) | 421, 1553 |
| RsaI | GTAC | 4 cut(s) | 488, 1038, 1167, 1231 |
| RsaNI | GTAC | 4 cut(s) | 487, 1037, 1166, 1230 |
| RseI | CAYNNNNRTG | 2 cut(s) | 815, 1400 |
| SacI | GAGCTC | 1 cut(s) | 1821 |
| SapI | GCTCTTC | 1 cut(s) | 311 |
| SaqAI | TTAA | 6 cut(s) | 246, 933, 1178, 1439, 1473, 1853 |
| Sau3AI | GATC | 6 cut(s) | 415, 490, 796, 978, 1562, 1649 |
| Sau96I | GGNCC | 1 cut(s) | 1698 |
| SchI | GAGTC | 4 cut(s) | 179, 436, 951, 1941 |
| ScrFI | CCNGG | 6 cut(s) | 407, 504, 1188, 1284, 1478, 1942 |
| SduI | GDGCHC | 2 cut(s) | 705, 1821 |
| SfaNI | GCATC | 7 cut(s) | 828, 1154, 1282, 1349, 1367, 1699, 1723 |
| SfcI | CTRYAG | 2 cut(s) | 1659, 1907 |
| SfuI | TTCGAA | 1 cut(s) | 1270 |
| SinI | GGWCC | 1 cut(s) | 1698 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 815, 1400 |
| SmlI | CTYRAG | 2 cut(s) | 737, 1775 |
| SmoI | CTYRAG | 2 cut(s) | 737, 1775 |
| SnaBI | TACGTA | 1 cut(s) | 1229 |
| SphI | GCATGC | 1 cut(s) | 343 |
| SspMI | CTAG | 3 cut(s) | 648, 1194, 1869 |
| SstI | GAGCTC | 1 cut(s) | 1821 |
| StyD4I | CCNGG | 6 cut(s) | 405, 502, 1186, 1282, 1476, 1940 |
| StyI | CCWWGG | 3 cut(s) | 204, 267, 356 |
| TaaI | ACNGT | 6 cut(s) | 709, 1102, 1212, 1234, 1488, 1660 |
| TaiI | ACGT | 2 cut(s) | 1231, 1922 |
| TatI | WGTACW | 2 cut(s) | 1036, 1165 |
| TauI | GCSGC | 4 cut(s) | 57, 285, 405, 1262 |
| TfiI | GAWTC | 4 cut(s) | 215, 750, 1241, 1953 |
| Tru1I | TTAA | 6 cut(s) | 246, 933, 1178, 1439, 1473, 1853 |
| Tru9I | TTAA | 6 cut(s) | 246, 933, 1178, 1439, 1473, 1853 |
| TscAI | CASTG | 2 cut(s) | 445, 1845 |
| TseFI | GTSAC | 1 cut(s) | 1459 |
| Tsp45I | GTSAC | 1 cut(s) | 1459 |
| TspDTI | ATGAA | 6 cut(s) | 491, 712, 919, 951, 1204, 1910 |
| TspRI | CASTG | 2 cut(s) | 445, 1845 |
| VpaK11BI | GGWCC | 1 cut(s) | 1698 |
| VspI | ATTAAT | 1 cut(s) | 933 |
| XapI | RAATTY | 6 cut(s) | 459, 469, 498, 596, 925, 1848 |
| XceI | RCATGY | 1 cut(s) | 343 |
| XmiI | GTMKAC | 1 cut(s) | 945 |
| XmnI | GAANNNNTTC | 1 cut(s) | 320 |
| XspI | CTAG | 3 cut(s) | 648, 1194, 1869 |
| Zsp2I | ATGCAT | 1 cut(s) | 1615 |