Rh6DG114100

protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0001220
Physical Location & Seq
Reverse (-)
15073909 .. 15077621
3713 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG114100.1

Sequence Viewer

Length: 1035 bp
ATGGCTGATGCCAGGCAGAAGACAACAAATATCTTTGCCCAATCCAAGTTGCGAAATAAAATCTCTCCTCCCATTCGTCTCTGTCTTCATCTTTCGTCTCTCCCATGTTACTCTTCATCTCGTTATGACCAGAAAAAACTTTCAATCCATTCAATAATTCTGGCAAGAGCAGAGATGGGTCTGGATTTGATCGACTCGTCGTCTCTCCCTCCGCGCCTTAGACGACTCCTCGTCCTCCGCCACCAAACCAGACGAATCGACCGAAAACGGCACCGTCAAGAAGGGGAGCGTGTCTTGACCATGACATTGTGCGGCCAGGTAAGTTTGTTGTGGCATATGCACCTGCTTGTAGTAAAAAAGAATATTACCTTGCTTCTGCTTGTCAAGAGATTTATGCCCCTCCAAGTGCTTAATTTTCTCTGTTTCGTCTCAGTGTTCAGGCATCATTTGTTAGAGGTGTTCTTGAGAAAATTGGAGTTGAGCCACAAGTGGAAAGGATTGGAAAGACTTGGAGTACAAAAACAGAAAAAGCTTCCTATGGTTGATTACTGGAAATATTCTAAGGTTCGGAAATGGACTCTTGGATTATCAGGCGGTAAAGACAAGATAGCCATAATAAGAGCCTCAGGCAGTATTAGCTGTGTACGAGATTCTTTTAGTCTACCTGGATCATCTCTTGTTGGAGAACAGTTCATTGAAAAGATCCGTACTATAAGAGAATCAAAAAGATACAAAGCTGCTATTATCCGAATTGACTGCCCTGGAGGTGATGCCCTTGCTTCTGATTTGATGTGGAGGGAAATCAAACTTTTGGCTGCATCAAAACCGGTCATTGTGTCAATGTCTGATGTGGCAGCAAGTGGAGGATACTATATGGCTATGGCAGCAGATGCCGTTGTTGCAGAAAATCTAACCTTAACTAGTTCCATTGGAGTTGTCACAGGAAAGTTTAACCTTGGAAAACTGTGCGAGAAGATTGGCTTCAACAAAGAAATCATATCACGGGGAAAAATTTGCAGATGTTCTTGCAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_S49

Protein Analysis

344

Amino Acids

38.9

Weight (kDa)

10.18

Isoelectric Point (pI)

46.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S49 PF01343 271 - 337 6.6e-19 Peptidase family S49
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 351
Acc36I ACCTGC 1 cut(s) 351
AccB1I GGYRCC 1 cut(s) 270
AccI GTMKAC 1 cut(s) 661
AccII CGCG 1 cut(s) 214
AciI CCGC 4 cut(s) 212, 238, 312, 594
AclWI GGATC 2 cut(s) 676, 697
AcoI YGGCCR 1 cut(s) 313
AcsI RAATTY 1 cut(s) 1011
AfaI GTAC 3 cut(s) 516, 645, 709
AgeI ACCGGT 1 cut(s) 826
AgsI TTSAA 4 cut(s) 144, 153, 698, 985
AhdI GACNNNNNGTC 2 cut(s) 199, 230
AhlI ACTAGT 1 cut(s) 920
AjnI CCWGG 4 cut(s) 11, 315, 664, 760
AluBI AGCT 4 cut(s) 532, 639, 737, 1032
AluI AGCT 4 cut(s) 532, 639, 737, 1032
Alw26I GTCTC 4 cut(s) 83, 102, 207, 433
AlwI GGATC 2 cut(s) 676, 697
AoxI GGCC 1 cut(s) 313
ApeKI GCWGC 5 cut(s) 737, 815, 854, 884, 1029
ApoI RAATTY 1 cut(s) 1011
AsiGI ACCGGT 1 cut(s) 826
AspLEI GCGC 1 cut(s) 216
AsuHPI GGTGA 1 cut(s) 779
AxyI CCTNAGG 1 cut(s) 625
BanI GGYRCC 1 cut(s) 270
BbsI GAAGAC 2 cut(s) 26, 77
BbvI GCAGC 4 cut(s) 724, 802, 866, 896
BccI CCATC 1 cut(s) 169
BceAI ACGGC 2 cut(s) 284, 878
BcgI CGANNNNNNTGC 2 cut(s) 738, 772
BciT130I CCWGG 4 cut(s) 13, 317, 666, 762
BciVI GTATCC 1 cut(s) 860
BcoDI GTCTC 4 cut(s) 83, 102, 207, 433
BcuI ACTAGT 1 cut(s) 920
BfaI CTAG 1 cut(s) 921
BfuAI ACCTGC 1 cut(s) 351
BfuI GTATCC 1 cut(s) 860
BisI GCNGC 6 cut(s) 313, 738, 816, 855, 885, 1030
BlsI GCNGC 6 cut(s) 314, 739, 817, 856, 886, 1031
Bme1390I CCNGG 4 cut(s) 13, 317, 666, 762
BmeRI GACNNNNNGTC 2 cut(s) 199, 230
BmiI GGNNCC 1 cut(s) 272
BmrFI CCNGG 4 cut(s) 13, 317, 666, 762
BmsI GCATC 4 cut(s) 451, 760, 827, 880
BpiI GAAGAC 2 cut(s) 26, 77
BpmI CTGGAG 1 cut(s) 783
BpuEI CTTGAG 1 cut(s) 484
BsaJI CCNNGG 2 cut(s) 760, 955
BsaWI WCCGGW 1 cut(s) 826
Bse118I RCCGGY 1 cut(s) 826
Bse1I ACTGG 1 cut(s) 554
Bse21I CCTNAGG 1 cut(s) 625
BseBI CCWGG 4 cut(s) 13, 317, 666, 762
BseDI CCNNGG 2 cut(s) 760, 955
BseMII CTCAG 2 cut(s) 444, 639
BseNI ACTGG 1 cut(s) 554
BseRI GAGGAG 2 cut(s) 57, 218
BseXI GCAGC 4 cut(s) 724, 802, 866, 896
Bsh1236I CGCG 1 cut(s) 214
Bsh1285I CGRYCG 1 cut(s) 262
BshFI GGCC 1 cut(s) 315
BshNI GGYRCC 1 cut(s) 270
BshTI ACCGGT 1 cut(s) 826
BsiEI CGRYCG 1 cut(s) 262
BsiSI CCGG 1 cut(s) 827
BsmAI GTCTC 4 cut(s) 83, 102, 207, 433
BsmBI CGTCTC 4 cut(s) 83, 102, 207, 433
BsnI GGCC 1 cut(s) 315
Bsp143I GATC 3 cut(s) 189, 668, 702
BspACI CCGC 4 cut(s) 212, 238, 312, 594
BspANI GGCC 1 cut(s) 315
BspCNI CTCAG 2 cut(s) 443, 638
BspFNI CGCG 1 cut(s) 214
BspLI GGNNCC 1 cut(s) 272
BspMI ACCTGC 1 cut(s) 351
BspPI GGATC 2 cut(s) 676, 697
BspT107I GGYRCC 1 cut(s) 270
BsrFI RCCGGY 1 cut(s) 826
BsrI ACTGG 1 cut(s) 554
BssAI RCCGGY 1 cut(s) 826
BssECI CCNNGG 2 cut(s) 760, 955
BssMI GATC 3 cut(s) 189, 668, 702
BssT1I CCWWGG 1 cut(s) 955
Bst2UI CCWGG 4 cut(s) 13, 317, 666, 762
Bst4CI ACNGT 3 cut(s) 275, 690, 966
Bst6I CTCTTC 1 cut(s) 118
BstAPI GCANNNNNTGC 1 cut(s) 890
BstDEI CTNAG 4 cut(s) 218, 430, 561, 625
BstFNI CGCG 1 cut(s) 214
BstHHI GCGC 1 cut(s) 216
BstKTI GATC 3 cut(s) 192, 671, 705
BstMAI GTCTC 4 cut(s) 83, 102, 207, 433
BstMBI GATC 3 cut(s) 189, 668, 702
BstMCI CGRYCG 1 cut(s) 262
BstMWI GCNNNNNNNGC 4 cut(s) 636, 884, 890, 899
BstNI CCWGG 4 cut(s) 13, 317, 666, 762
BstSCI CCNGG 4 cut(s) 11, 315, 664, 760
BstUI CGCG 1 cut(s) 214
BstV1I GCAGC 4 cut(s) 724, 802, 866, 896
BstV2I GAAGAC 2 cut(s) 26, 77
BstX2I RGATCY 1 cut(s) 702
BstYI RGATCY 1 cut(s) 702
Bsu36I CCTNAGG 1 cut(s) 625
BsuI GTATCC 1 cut(s) 860
BsuRI GGCC 1 cut(s) 315
BtsIMutI CAGTG 1 cut(s) 438
BveI ACCTGC 1 cut(s) 351
CfoI GCGC 1 cut(s) 216
Cfr10I RCCGGY 1 cut(s) 826
Csp6I GTAC 3 cut(s) 515, 644, 708
CspAI ACCGGT 1 cut(s) 826
CviAII CATG 2 cut(s) 105, 301
CviQI GTAC 3 cut(s) 515, 644, 708
DdeI CTNAG 4 cut(s) 218, 430, 561, 625
DpnI GATC 3 cut(s) 191, 670, 704
DpnII GATC 3 cut(s) 189, 668, 702
DriI GACNNNNNGTC 2 cut(s) 199, 230
EaeI YGGCCR 1 cut(s) 313
Eam1104I CTCTTC 1 cut(s) 118
Eam1105I GACNNNNNGTC 2 cut(s) 199, 230
EarI CTCTTC 1 cut(s) 118
EciI GGCGGA 1 cut(s) 227
Eco130I CCWWGG 1 cut(s) 955
Eco81I CCTNAGG 1 cut(s) 625
EcoRII CCWGG 4 cut(s) 11, 315, 664, 760
EcoT14I CCWWGG 1 cut(s) 955
ErhI CCWWGG 1 cut(s) 955
Esp3I CGTCTC 4 cut(s) 83, 102, 207, 433
FaeI CATG 2 cut(s) 108, 304
FalI AAGNNNNNCTT 2 cut(s) 965, 997
FatI CATG 2 cut(s) 104, 300
FauNDI CATATG 1 cut(s) 336
FblI GTMKAC 1 cut(s) 661
Fnu4HI GCNGC 6 cut(s) 313, 738, 816, 855, 885, 1030
Fsp4HI GCNGC 6 cut(s) 313, 738, 816, 855, 885, 1030
FspBI CTAG 1 cut(s) 921
GlaI GCGC 1 cut(s) 215
GluI GCNGC 6 cut(s) 313, 738, 816, 855, 885, 1030
GsuI CTGGAG 1 cut(s) 783
HaeIII GGCC 1 cut(s) 315
HapII CCGG 1 cut(s) 827
HhaI GCGC 1 cut(s) 216
Hin1II CATG 2 cut(s) 108, 304
Hin6I GCGC 1 cut(s) 214
HinP1I GCGC 1 cut(s) 214
HindIII AAGCTT 1 cut(s) 530
HinfI GANTC 6 cut(s) 194, 225, 255, 577, 650, 719
HpaII CCGG 1 cut(s) 827
HphI GGTGA 1 cut(s) 779
Hpy166II GTNNAC 2 cut(s) 644, 662
Hpy188I TCNGA 4 cut(s) 570, 749, 784, 847
Hpy188III TCNNGA 5 cut(s) 182, 278, 295, 385, 463
Hpy8I GTNNAC 2 cut(s) 644, 662
Hpy99I CGWCG 1 cut(s) 202
HpyAV CCTTC 1 cut(s) 275
HpyCH4III ACNGT 3 cut(s) 275, 690, 966
HpyCH4V TGCA 5 cut(s) 340, 818, 902, 1017, 1029
HpyF10VI GCNNNNNNNGC 4 cut(s) 636, 884, 890, 899
HpyF3I CTNAG 4 cut(s) 218, 430, 561, 625
Hsp92II CATG 2 cut(s) 108, 304
HspAI GCGC 1 cut(s) 214
Kzo9I GATC 3 cut(s) 189, 668, 702
LmnI GCTCC 1 cut(s) 286
Lsp1109I GCAGC 4 cut(s) 724, 802, 866, 896
LweI GCATC 4 cut(s) 451, 760, 827, 880
MaeI CTAG 1 cut(s) 921
MaeIII GTNAC 2 cut(s) 107, 937
MalI GATC 3 cut(s) 191, 670, 704
MboI GATC 3 cut(s) 189, 668, 702
MboII GAAGA 4 cut(s) 31, 77, 105, 985
MflI RGATCY 1 cut(s) 702
MluCI AATT 5 cut(s) 156, 412, 470, 750, 1011
MlyI GAGTC 3 cut(s) 188, 219, 571
MmeI TCCRAC 1 cut(s) 661
MseI TTAA 3 cut(s) 411, 917, 951
MspA1I CMGCKG 1 cut(s) 1032
MspI CCGG 1 cut(s) 827
MspR9I CCNGG 4 cut(s) 13, 317, 666, 762
MvaI CCWGG 4 cut(s) 13, 317, 666, 762
MvnI CGCG 1 cut(s) 214
MwoI GCNNNNNNNGC 4 cut(s) 636, 884, 890, 899
NdeI CATATG 1 cut(s) 336
NdeII GATC 3 cut(s) 189, 668, 702
NlaIII CATG 2 cut(s) 108, 304
NlaIV GGNNCC 1 cut(s) 272
NmuCI GTSAC 1 cut(s) 937
PaqCI CACCTGC 1 cut(s) 351
PcsI WCGNNNNNNNCGW 1 cut(s) 259
PfeI GAWTC 3 cut(s) 255, 650, 719
PinAI ACCGGT 1 cut(s) 826
PkrI GCNGC 6 cut(s) 314, 739, 817, 856, 886, 1031
PleI GAGTC 3 cut(s) 188, 219, 571
PpsI GAGTC 3 cut(s) 188, 219, 571
Psp6I CCWGG 4 cut(s) 11, 315, 664, 760
PspGI CCWGG 4 cut(s) 11, 315, 664, 760
PspN4I GGNNCC 1 cut(s) 272
PsuI RGATCY 1 cut(s) 702
PvuII CAGCTG 1 cut(s) 1032
RsaI GTAC 3 cut(s) 516, 645, 709
RsaNI GTAC 3 cut(s) 515, 644, 708
SaqAI TTAA 3 cut(s) 411, 917, 951
SatI GCNGC 6 cut(s) 313, 738, 816, 855, 885, 1030
Sau3AI GATC 3 cut(s) 189, 668, 702
SchI GAGTC 3 cut(s) 188, 219, 571
ScrFI CCNGG 4 cut(s) 13, 317, 666, 762
SfaNI GCATC 4 cut(s) 451, 760, 827, 880
SmlI CTYRAG 1 cut(s) 463
SmoI CTYRAG 1 cut(s) 463
SpeI ACTAGT 1 cut(s) 920
Sse9I AATT 5 cut(s) 156, 412, 470, 750, 1011
SsiI CCGC 4 cut(s) 212, 238, 312, 594
SspI AATATT 2 cut(s) 364, 557
SspMI CTAG 1 cut(s) 921
StyD4I CCNGG 4 cut(s) 11, 315, 664, 760
StyI CCWWGG 1 cut(s) 955
TaaI ACNGT 3 cut(s) 275, 690, 966
TaqI TCGA 2 cut(s) 192, 258
TaqII GACCGA 1 cut(s) 276
TasI AATT 5 cut(s) 156, 412, 470, 750, 1011
TatI WGTACW 1 cut(s) 514
TauI GCSGC 1 cut(s) 315
TfiI GAWTC 3 cut(s) 255, 650, 719
Tru1I TTAA 3 cut(s) 411, 917, 951
Tru9I TTAA 3 cut(s) 411, 917, 951
TscAI CASTG 1 cut(s) 438
TseFI GTSAC 1 cut(s) 937
TseI GCWGC 5 cut(s) 737, 815, 854, 884, 1029
Tsp45I GTSAC 1 cut(s) 937
TspDTI ATGAA 3 cut(s) 77, 105, 682
TspGWI ACGGA 1 cut(s) 695
TspRI CASTG 1 cut(s) 438
XapI RAATTY 1 cut(s) 1011
XmiI GTMKAC 1 cut(s) 661
XspI CTAG 1 cut(s) 921
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.