Rh7BG063200

protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0001220
Physical Location & Seq
Reverse (-)
4218056 .. 4221819
3764 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG063200.1

Sequence Viewer

Length: 1884 bp
ATGTCGAAGCTGATTCTCCTCCACGCTTCCCACCACCTCACCCCAATCCACACCCGAGCCTTCAACGCCGTCGTCGGAGCCTCTCTCTCCACGCCGCTCCTCCACTCTCACTGCCGTCCTTTCTTCTCTCACTCTCCTCCTCCTCTCTCTCTCCGACGCCGCCTCTCCCTCCGCGCCTTCGACGACTCGTCGTCGTCCGCCACCAAACCAGACGAATCGACCGAAAACGGCGCCGTTTCAGCTCCCGAGGCTAAAATCGACGCCGAGAATGCCAAAGCTACGACGGACAAGGACTATCCGAGCGGCGAGTTTCAGTTTCAGGAGGCGAGCCTCTGGAAGAGCTTCGTCGTCAAGCTTCGGATGCTCTTTGCTTATCCTTGGGAGCGGGTCAAGAAGGGAAGCGTCTTGACCATGACATTGCGCGGCCAGATAACCGATCAGTTAAAGAGTCGATTCTCCAGTGGATTATCGCTGCCTCAAATTTGTGAGAATTTTGTGAAAGCTGCGTATGATCCTCGAATTGCTGGTGTGTATCTTCAAATTGAGAGCTTGAACTGTGGATGGGGTAAAGTGGAAGAAATTCGGAGGCATATATTGGACTTCCAGAAATCAGGTAAGTTTGTTGTGGCATACGCACCTGCTTGTAGCGAAAAAGAGTATTACCTTGCTTCTGCTTGTCAGGAGATTTATGCCCCTCCGAGTGCTTATTTTTCCTTGTTTGGTCTCAGTGTTCAGGCATCATTTGTAAGAGGTGTTCTTGAGAAAATTGGAGTTGAGCCACAAGTGGAAAGGATTGGTAAATATAAAAGTGCTGGTGACCAACTTGCGCGAACGACCATGTCCGAAGAAAATTGTGAGATGCTGACAGCATTGCTCGATAACATATATGGGAACTGGCTGGACATAGTTTCTTCTACAAGAGGAAAAGAGAGAGAAGATATTGAGAAGTTTGTTAATGAAGGGGTGTACCAAGTAGAAAAGCTGAAAGAAGAGGGGTGGATCACAAACATACAGTATGATGATGAGGTTACTTCAATGTTGAAGGAAAGACTTGGAGTACAAAAAGAGAAAAAACTTCCTATGGTTGATTACAGGAAATATTCTAAGGTCCGGAAATGGACTCTTGGTTTATCAGGCGGTAAAGACAAGATAGCCATAATAAGAGCCTCAGGCAGTATTAGTCGTGTACGAGGTTCTTTTAGTCTACCTGGATCATCTATTGTTGGAGAACAGTTCATTGAGAAGATCCGTACTATAAGAGAATCAAAAAGATACAAGGCTGCTATTATCCGAATTGACAGCCCTGGAGGTGATGCCCTTGCTTCTGATTTGATGTGGAGGGAAATCAAACTTTTGGCTGCATCAAAACCGGTCATTGCATCAATGTCTGATGTGGCAGCAAGTGGAGGATACTATATGGCTATGGCAGCAGATGCCATTGTTGCAGAAAATCTAACCTTAACTGGTTCCATTGGAGTTGTCACAGGGAAGTTTAACCTGGGAAAACTGTATGAGAAGATTGGCTTCAACAAGGAAATCATATCAAGGGGGAAATTTGCAGAGGTTCTTGCAGCTGAACAACGACCCTTCAAACCAGAGGAAGCTGAACTCTTTGCTAAGTCTGCACAGAATTCGTATAAGCAATTTCGGGACAAGGCAGCTTCTTCCAGATCAATGACTGTAGATAAAATGGAGGAAGTTGCACAAGGGAGAGTTTGGGCTGGTAAAGATGCAGCTTCGAGGGGTTTAGTTGATGCCATTGGTGGACTTTCTCGGGCTGTTGCAATAGCAAAGCTCAAGGCAAATATACCACAAGACACAGAGGTTGGTGACAAAAATCTTATTTCAGTGGCTAGGCATATACTAGAGATAATAATAAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_S49

Protein Analysis

627

Amino Acids

69.33

Weight (kDa)

9.14

Isoelectric Point (pI)

40.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S49 PF01343 201 - 353 2.6e-24 Peptidase family S49
Peptidase_S49 PF01343 452 - 602 2.5e-42 Peptidase family S49
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 646
Acc36I ACCTGC 1 cut(s) 646
AccB1I GGYRCC 1 cut(s) 230
AccBSI CCGCTC 3 cut(s) 97, 303, 385
AccI GTMKAC 1 cut(s) 1204
AccII CGCG 3 cut(s) 174, 423, 829
AccIII TCCGGA 1 cut(s) 1110
AciI CCGC 8 cut(s) 95, 160, 172, 198, 303, 385, 423, 1137
AclWI GGATC 4 cut(s) 506, 1007, 1219, 1240
AcoI YGGCCR 1 cut(s) 424
AcsI RAATTY 5 cut(s) 480, 490, 579, 1553, 1630
AcyI GRCGYC 3 cut(s) 157, 231, 261
AfaI GTAC 4 cut(s) 968, 1059, 1188, 1252
AgeI ACCGGT 1 cut(s) 1369
AgsI TTSAA 7 cut(s) 64, 539, 553, 1035, 1042, 1528, 1591
AhdI GACNNNNNGTC 2 cut(s) 187, 190
AjnI CCWGG 3 cut(s) 1207, 1303, 1497
Alw26I GTCTC 1 cut(s) 728
AlwI GGATC 4 cut(s) 506, 1007, 1219, 1240
Ama87I CYCGRG 3 cut(s) 54, 245, 1773
Aor13HI TCCGGA 1 cut(s) 1110
AoxI GGCC 1 cut(s) 424
ApeKI GCWGC 9 cut(s) 472, 503, 1280, 1358, 1397, 1427, 1571, 1658, 1733
ApoI RAATTY 5 cut(s) 480, 490, 579, 1553, 1630
AsiGI ACCGGT 1 cut(s) 1369
Asp700I GAANNNNTTC 2 cut(s) 341, 579
AspLEI GCGC 4 cut(s) 176, 233, 423, 829
AspS9I GGNCC 1 cut(s) 1108
AsuHPI GGTGA 4 cut(s) 31, 827, 1322, 1841
AvaI CYCGRG 3 cut(s) 54, 245, 1773
AvaII GGWCC 1 cut(s) 1108
AxyI CCTNAGG 1 cut(s) 1168
BanI GGYRCC 1 cut(s) 230
BbvI GCAGC 9 cut(s) 459, 490, 1267, 1345, 1409, 1439, 1583, 1670, 1745
BccI CCATC 1 cut(s) 555
BceAI ACGGC 4 cut(s) 53, 99, 218, 244
BcgI CGANNNNNNTGC 2 cut(s) 1614, 1648
BciT130I CCWGG 3 cut(s) 1209, 1305, 1499
BciVI GTATCC 1 cut(s) 1403
BcoDI GTCTC 1 cut(s) 728
BfaI CTAG 2 cut(s) 1854, 1865
BfmI CTRYAG 1 cut(s) 1680
BfoI RGCGCY 1 cut(s) 234
BfuAI ACCTGC 1 cut(s) 646
BfuI GTATCC 1 cut(s) 1403
Bme1390I CCNGG 3 cut(s) 1209, 1305, 1499
Bme18I GGWCC 1 cut(s) 1108
BmeRI GACNNNNNGTC 2 cut(s) 187, 190
BmeT110I CYCGRG 3 cut(s) 54, 245, 1773
BmgT120I GGNCC 1 cut(s) 1108
BmiI GGNNCC 3 cut(s) 79, 232, 1468
BmrFI CCNGG 3 cut(s) 1209, 1305, 1499
BmsI GCATC 9 cut(s) 351, 746, 849, 1303, 1370, 1388, 1423, 1720, 1744
BplI GAGNNNNNCTC 2 cut(s) 69, 101
BpmI CTGGAG 2 cut(s) 442, 1326
BpuEI CTTGAG 2 cut(s) 779, 1781
BsaHI GRCGYC 3 cut(s) 157, 231, 261
BsaI GGTCTC 1 cut(s) 728
BsaJI CCNNGG 4 cut(s) 246, 377, 1303, 1498
BsaWI WCCGGW 2 cut(s) 1110, 1369
Bse118I RCCGGY 1 cut(s) 1369
Bse1I ACTGG 3 cut(s) 459, 899, 1468
Bse21I CCTNAGG 1 cut(s) 1168
Bse3DI GCAATG 3 cut(s) 416, 869, 1374
BseAI TCCGGA 1 cut(s) 1110
BseBI CCWGG 3 cut(s) 1209, 1305, 1499
BseDI CCNNGG 4 cut(s) 246, 377, 1303, 1498
BseGI GGATG 2 cut(s) 366, 566
BseMI GCAATG 3 cut(s) 416, 869, 1374
BseMII CTCAG 2 cut(s) 739, 1182
BseNI ACTGG 3 cut(s) 459, 899, 1468
BseRI GAGGAG 5 cut(s) 8, 89, 126, 129, 132
BseXI GCAGC 9 cut(s) 459, 490, 1267, 1345, 1409, 1439, 1583, 1670, 1745
BsgI GTGCAG 1 cut(s) 1608
Bsh1236I CGCG 3 cut(s) 174, 423, 829
Bsh1285I CGRYCG 1 cut(s) 222
BshFI GGCC 1 cut(s) 426
BshNI GGYRCC 1 cut(s) 230
BshTI ACCGGT 1 cut(s) 1369
BsiEI CGRYCG 1 cut(s) 222
BsiHKCI CYCGRG 3 cut(s) 54, 245, 1773
BsiSI CCGG 2 cut(s) 1111, 1370
BslFI GGGAC 1 cut(s) 1664
BsmAI GTCTC 1 cut(s) 728
BsmFI GGGAC 1 cut(s) 1664
BsmI GAATGC 1 cut(s) 274
BsnI GGCC 1 cut(s) 426
Bso31I GGTCTC 1 cut(s) 728
BsoBI CYCGRG 3 cut(s) 54, 245, 1773
Bsp13I TCCGGA 1 cut(s) 1110
Bsp143I GATC 6 cut(s) 436, 511, 999, 1211, 1245, 1670
BspACI CCGC 8 cut(s) 95, 160, 172, 198, 303, 385, 423, 1137
BspANI GGCC 1 cut(s) 426
BspCNI CTCAG 2 cut(s) 738, 1181
BspEI TCCGGA 1 cut(s) 1110
BspFNI CGCG 3 cut(s) 174, 423, 829
BspLI GGNNCC 3 cut(s) 79, 232, 1468
BspMI ACCTGC 1 cut(s) 646
BspPI GGATC 4 cut(s) 506, 1007, 1219, 1240
BspQI GCTCTTC 1 cut(s) 332
BspT107I GGYRCC 1 cut(s) 230
BspTNI GGTCTC 1 cut(s) 728
BsrBI CCGCTC 3 cut(s) 97, 303, 385
BsrDI GCAATG 3 cut(s) 416, 869, 1374
BsrFI RCCGGY 1 cut(s) 1369
BsrI ACTGG 3 cut(s) 459, 899, 1468
BssAI RCCGGY 1 cut(s) 1369
BssECI CCNNGG 4 cut(s) 246, 377, 1303, 1498
BssMI GATC 6 cut(s) 436, 511, 999, 1211, 1245, 1670
BssNI GRCGYC 3 cut(s) 157, 231, 261
BssT1I CCWWGG 1 cut(s) 377
Bst2UI CCWGG 3 cut(s) 1209, 1305, 1499
Bst4CI ACNGT 5 cut(s) 557, 1014, 1233, 1509, 1681
Bst6I CTCTTC 2 cut(s) 332, 984
BstACI GRCGYC 3 cut(s) 157, 231, 261
BstAPI GCANNNNNTGC 1 cut(s) 1433
BstC8I GCNNGC 1 cut(s) 328
BstDEI CTNAG 4 cut(s) 725, 1104, 1168, 1617
BstEII GGTNACC 1 cut(s) 815
BstF5I GGATG 2 cut(s) 366, 566
BstFNI CGCG 3 cut(s) 174, 423, 829
BstH2I RGCGCY 1 cut(s) 234
BstHHI GCGC 4 cut(s) 176, 233, 423, 829
BstKTI GATC 6 cut(s) 439, 514, 1002, 1214, 1248, 1673
BstMAI GTCTC 1 cut(s) 728
BstMBI GATC 6 cut(s) 436, 511, 999, 1211, 1245, 1670
BstMCI CGRYCG 1 cut(s) 222
BstMWI GCNNNNNNNGC 9 cut(s) 65, 239, 248, 269, 361, 1427, 1433, 1442, 1622
BstNI CCWGG 3 cut(s) 1209, 1305, 1499
BstPI GGTNACC 1 cut(s) 815
BstSCI CCNGG 3 cut(s) 1207, 1303, 1497
BstSFI CTRYAG 1 cut(s) 1680
BstUI CGCG 3 cut(s) 174, 423, 829
BstV1I GCAGC 9 cut(s) 459, 490, 1267, 1345, 1409, 1439, 1583, 1670, 1745
BstX2I RGATCY 1 cut(s) 1245
BstYI RGATCY 1 cut(s) 1245
Bsu36I CCTNAGG 1 cut(s) 1168
BsuI GTATCC 1 cut(s) 1403
BsuRI GGCC 1 cut(s) 426
BtsCI GGATG 2 cut(s) 366, 566
BtsI GCAGTG 1 cut(s) 109
BtsIMutI CAGTG 4 cut(s) 109, 466, 733, 1854
BveI ACCTGC 1 cut(s) 646
Cac8I GCNNGC 1 cut(s) 328
CfoI GCGC 4 cut(s) 176, 233, 423, 829
Cfr10I RCCGGY 1 cut(s) 1369
Cfr13I GGNCC 1 cut(s) 1108
CseI GACGC 3 cut(s) 165, 269, 391
Csp6I GTAC 4 cut(s) 967, 1058, 1187, 1251
CspAI ACCGGT 1 cut(s) 1369
CviAII CATG 2 cut(s) 412, 838
CviQI GTAC 4 cut(s) 967, 1058, 1187, 1251
DdeI CTNAG 4 cut(s) 725, 1104, 1168, 1617
DinI GGCGCC 1 cut(s) 232
DpnI GATC 6 cut(s) 438, 513, 1001, 1213, 1247, 1672
DpnII GATC 6 cut(s) 436, 511, 999, 1211, 1245, 1670
DriI GACNNNNNGTC 2 cut(s) 187, 190
EaeI YGGCCR 1 cut(s) 424
Eam1104I CTCTTC 2 cut(s) 332, 984
Eam1105I GACNNNNNGTC 2 cut(s) 187, 190
EarI CTCTTC 2 cut(s) 332, 984
EciI GGCGGA 1 cut(s) 187
Eco130I CCWWGG 1 cut(s) 377
Eco31I GGTCTC 1 cut(s) 728
Eco47I GGWCC 1 cut(s) 1108
Eco81I CCTNAGG 1 cut(s) 1168
Eco88I CYCGRG 3 cut(s) 54, 245, 1773
Eco91I GGTNACC 1 cut(s) 815
EcoO65I GGTNACC 1 cut(s) 815
EcoRI GAATTC 1 cut(s) 1630
EcoRII CCWGG 3 cut(s) 1207, 1303, 1497
EcoT14I CCWWGG 1 cut(s) 377
EgeI GGCGCC 1 cut(s) 232
EheI GGCGCC 1 cut(s) 232
ErhI CCWWGG 1 cut(s) 377
FaeI CATG 2 cut(s) 415, 841
FalI AAGNNNNNCTT 2 cut(s) 1508, 1540
FaqI GGGAC 1 cut(s) 1664
FatI CATG 2 cut(s) 411, 837
FauI CCCGC 1 cut(s) 378
FblI GTMKAC 1 cut(s) 1204
FokI GGATG 2 cut(s) 373, 573
FspBI CTAG 2 cut(s) 1854, 1865
GlaI GCGC 4 cut(s) 175, 232, 422, 828
GsuI CTGGAG 2 cut(s) 442, 1326
HaeII RGCGCY 1 cut(s) 234
HaeIII GGCC 1 cut(s) 426
HapII CCGG 2 cut(s) 1111, 1370
HgaI GACGC 3 cut(s) 165, 269, 391
HhaI GCGC 4 cut(s) 176, 233, 423, 829
Hin1I GRCGYC 3 cut(s) 157, 231, 261
Hin1II CATG 2 cut(s) 415, 841
Hin6I GCGC 4 cut(s) 174, 231, 421, 827
HinP1I GCGC 4 cut(s) 174, 231, 421, 827
HindIII AAGCTT 1 cut(s) 353
HinfI GANTC 7 cut(s) 13, 185, 215, 448, 453, 1120, 1262
HpaII CCGG 2 cut(s) 1111, 1370
HphI GGTGA 4 cut(s) 31, 827, 1322, 1841
Hpy166II GTNNAC 4 cut(s) 967, 1187, 1205, 1766
Hpy8I GTNNAC 4 cut(s) 967, 1187, 1205, 1766
Hpy99I CGWCG 9 cut(s) 74, 77, 159, 185, 193, 196, 263, 286, 350
HpyAV CCTTC 6 cut(s) 70, 187, 388, 953, 1036, 1597
HpyCH4III ACNGT 5 cut(s) 557, 1014, 1233, 1509, 1681
HpyCH4V TGCA 9 cut(s) 1361, 1379, 1445, 1559, 1571, 1625, 1703, 1733, 1784
HpyF10VI GCNNNNNNNGC 9 cut(s) 65, 239, 248, 269, 361, 1427, 1433, 1442, 1622
HpyF3I CTNAG 4 cut(s) 725, 1104, 1168, 1617
Hsp92I GRCGYC 3 cut(s) 157, 231, 261
Hsp92II CATG 2 cut(s) 415, 841
HspAI GCGC 4 cut(s) 174, 231, 421, 827
KasI GGCGCC 1 cut(s) 230
Kpn2I TCCGGA 1 cut(s) 1110
Kzo9I GATC 6 cut(s) 436, 511, 999, 1211, 1245, 1670
LguI GCTCTTC 1 cut(s) 332
LmnI GCTCC 4 cut(s) 77, 102, 247, 382
Lsp1109I GCAGC 9 cut(s) 459, 490, 1267, 1345, 1409, 1439, 1583, 1670, 1745
LweI GCATC 9 cut(s) 351, 746, 849, 1303, 1370, 1388, 1423, 1720, 1744
MaeI CTAG 2 cut(s) 1854, 1865
MaeIII GTNAC 4 cut(s) 815, 1027, 1480, 1829
MalI GATC 6 cut(s) 438, 513, 1001, 1213, 1247, 1672
MbiI CCGCTC 3 cut(s) 97, 303, 385
MboI GATC 6 cut(s) 436, 511, 999, 1211, 1245, 1670
MflI RGATCY 1 cut(s) 1245
Mly113I GGCGCC 1 cut(s) 231
MlyI GAGTC 3 cut(s) 179, 457, 1114
MmeI TCCRAC 3 cut(s) 55, 178, 1204
MroI TCCGGA 1 cut(s) 1110
MroXI GAANNNNTTC 2 cut(s) 341, 579
MseI TTAA 5 cut(s) 443, 954, 1460, 1494, 1882
MspA1I CMGCKG 1 cut(s) 1574
MspI CCGG 2 cut(s) 1111, 1370
MspR9I CCNGG 3 cut(s) 1209, 1305, 1499
Mva1269I GAATGC 1 cut(s) 274
MvaI CCWGG 3 cut(s) 1209, 1305, 1499
MvnI CGCG 3 cut(s) 174, 423, 829
MwoI GCNNNNNNNGC 9 cut(s) 65, 239, 248, 269, 361, 1427, 1433, 1442, 1622
NarI GGCGCC 1 cut(s) 231
NdeII GATC 6 cut(s) 436, 511, 999, 1211, 1245, 1670
NlaIII CATG 2 cut(s) 415, 841
NlaIV GGNNCC 3 cut(s) 79, 232, 1468
NmeAIII GCCGAG 1 cut(s) 289
NmuCI GTSAC 3 cut(s) 815, 1480, 1829
PaqCI CACCTGC 1 cut(s) 646
PciSI GCTCTTC 1 cut(s) 332
PcsI WCGNNNNNNNCGW 1 cut(s) 219
PctI GAATGC 1 cut(s) 274
PdmI GAANNNNTTC 2 cut(s) 341, 579
PfeI GAWTC 4 cut(s) 13, 215, 453, 1262
PflFI GACNNNGTC 1 cut(s) 838
PinAI ACCGGT 1 cut(s) 1369
PleI GAGTC 3 cut(s) 179, 456, 1114
PluTI GGCGCC 1 cut(s) 234
PpsI GAGTC 3 cut(s) 179, 456, 1114
Psp6I CCWGG 3 cut(s) 1207, 1303, 1497
PspEI GGTNACC 1 cut(s) 815
PspGI CCWGG 3 cut(s) 1207, 1303, 1497
PspN4I GGNNCC 3 cut(s) 79, 232, 1468
PspPI GGNCC 1 cut(s) 1108
PsrI GAACNNNNNNTAC 2 cut(s) 738, 770
PsuI RGATCY 1 cut(s) 1245
PsyI GACNNNGTC 1 cut(s) 838
PvuII CAGCTG 1 cut(s) 1574
RsaI GTAC 4 cut(s) 968, 1059, 1188, 1252
RsaNI GTAC 4 cut(s) 967, 1058, 1187, 1251
SapI GCTCTTC 1 cut(s) 332
SaqAI TTAA 5 cut(s) 443, 954, 1460, 1494, 1882
Sau3AI GATC 6 cut(s) 436, 511, 999, 1211, 1245, 1670
Sau96I GGNCC 1 cut(s) 1108
SchI GAGTC 3 cut(s) 179, 457, 1114
ScrFI CCNGG 3 cut(s) 1209, 1305, 1499
SfaNI GCATC 9 cut(s) 351, 746, 849, 1303, 1370, 1388, 1423, 1720, 1744
SfcI CTRYAG 1 cut(s) 1680
SfoI GGCGCC 1 cut(s) 232
SinI GGWCC 1 cut(s) 1108
SmlI CTYRAG 2 cut(s) 758, 1796
SmoI CTYRAG 2 cut(s) 758, 1796
SsiI CCGC 8 cut(s) 95, 160, 172, 198, 303, 385, 423, 1137
SspDI GGCGCC 1 cut(s) 230
SspI AATATT 1 cut(s) 1100
SspMI CTAG 2 cut(s) 1854, 1865
StyD4I CCNGG 3 cut(s) 1207, 1303, 1497
StyI CCWWGG 1 cut(s) 377
TaaI ACNGT 5 cut(s) 557, 1014, 1233, 1509, 1681
TaqI TCGA 8 cut(s) 5, 180, 218, 258, 451, 517, 876, 1739
TaqII GACCGA 1 cut(s) 236
TatI WGTACW 1 cut(s) 1057
TauI GCSGC 4 cut(s) 97, 162, 306, 426
TfiI GAWTC 4 cut(s) 13, 215, 453, 1262
Tru1I TTAA 5 cut(s) 443, 954, 1460, 1494, 1882
Tru9I TTAA 5 cut(s) 443, 954, 1460, 1494, 1882
TscAI CASTG 4 cut(s) 116, 466, 733, 1854
TseFI GTSAC 3 cut(s) 815, 1480, 1829
TseI GCWGC 9 cut(s) 472, 503, 1280, 1358, 1397, 1427, 1571, 1658, 1733
Tsp45I GTSAC 3 cut(s) 815, 1480, 1829
TspDTI ATGAA 2 cut(s) 972, 1225
TspGWI ACGGA 2 cut(s) 299, 1238
TspRI CASTG 4 cut(s) 116, 466, 733, 1854
Tth111I GACNNNGTC 1 cut(s) 838
VpaK11BI GGWCC 1 cut(s) 1108
XapI RAATTY 5 cut(s) 480, 490, 579, 1553, 1630
XmiI GTMKAC 1 cut(s) 1204
XmnI GAANNNNTTC 2 cut(s) 341, 579
XspI CTAG 2 cut(s) 1854, 1865
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.