Rroxscaffold_176G00431720

protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000176
OG0001220
Physical Location & Seq
Reverse (-)
1571833 .. 1574696
2864 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_176G00431720.1

Sequence Viewer

Length: 1389 bp
ATGCCAAAGCTACGACGGACAAGGACTATCCGAGCGGCGAGTTTCAGTTTCAGGAGGCGAGCCTGTGGAAGAGCTCCGAATGATCTTTGCTTATCCTTGGGAGCGGGTCAAGAAGGGAAGCGTGTCTTGACCATGACATTGTGCGGCCAGAGTCCATTCTCCAGTGGATTATCGCTGCCTCAAATTTGTGAGAATTTTGTGATGCTCGAATTACTGGTGTGTATGTATCTTCAAATTGAGAGCTTGAACTTTGGTTGGGGTAAAGTGGAGGAAATTCGGAGACATATATTGGACTTCCAGAAATCAGGTAATTTTGTTGTGGCATATGCGCCTGCTTGTAGCGAAAAAGAATATTACCTTGCCTCTGCTTGTCAAGAGATTTATGCCCCTCCAAATGCTTATTTTTCTTTGTTTGGTCTCAGTGTTCAGGCATCATTTGTAAGAGGTGTTCTTGAGAAAATTGGAGTTGAGCCACAAGTGGAAAGGATTGGTAGATATATAAGTACTGGTGACCAACTTGCACGCACGACCATGTCCGAAGAAAATTGTGAGATGCTAACAGCATTGCTCGATAACATATATTGGAAATTGCTGGACATAATTTCTTCTACAAGAGAAGCTATTGAGAAGTCTGTTAATGAAGGGGTGTGCCAAGTTGAGAAGCTGAAAGAAGAGGGCTGGATCACAAACATACAGTATGATGATGAGGGCTCTTACGGGTTACGAATTTTGGATATGAAGTATTTAAGTAATGTGCATTCCTTCCATGTTACTTCAATGTTGAAGGAAAGACTTGGAGGACAAAAAGAGAAAAAGCTTCCTATGGTTGATTACAGCCTGCGTCTTATGATATTCTTTTGTCCATCTCCAGAATCAAAAAGATACAAGGCTGCTATTATCCGACTTGACTGCCCTGGAGGTGATGCCCTTGCTTCTGATTTGATGTGGAGGGAAATCAAACTTTTGGCTGCATCAAAACCGGTCATTGCGTCAATGTCTGATGTGGCAGCAAGTGGAGGATACTACATGGCTACGGCAGCAGATGCCATTGTTGCAGAAAATCTAACCTCAACTGGTTCCATTGGAGGAAGTTTAACCTTGGAAAACTGTATGAGGAGATTGGCTTCAACAAAGAAATCATACCATGGGGAAAAATTTGCTGAGGTTCTTGCAGCTGAACAACGACCCTTCAGGCCAGAGGAATCTGAACTCTTTGCTAAGTCTGCACAGAATTCATATAAGCAATTTCGGGACAAGGCAGCGTCTCCCAGATCAATGACTGTAGATAAAATGGAGGAAGTTGCACAATTGAGAGTTTGGACTGGTAAAGATGCAGTTTCCAGGGTTTTAGTTGATGCTATTGTGGACTTCCTCGGGCTGTTGCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_S49

Protein Analysis

462

Amino Acids

51.59

Weight (kDa)

7.88

Isoelectric Point (pI)

52.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S49 PF01343 100 - 210 1.9e-15 Peptidase family S49
Peptidase_S49 PF01343 322 - 455 2.2e-28 Peptidase family S49
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 2 cut(s) 35, 104
AciI CCGC 3 cut(s) 35, 104, 144
AclWI GGATC 1 cut(s) 689
AcoI YGGCCR 1 cut(s) 145
AcsI RAATTY 6 cut(s) 183, 193, 273, 726, 1154, 1231
AcuI CTGAAG 1 cut(s) 1174
AfaI GTAC 1 cut(s) 505
AgeI ACCGGT 1 cut(s) 979
AgsI TTSAA 5 cut(s) 233, 247, 777, 784, 1128
AjnI CCWGG 2 cut(s) 913, 1340
AluBI AGCT 7 cut(s) 10, 74, 243, 620, 664, 817, 1175
AluI AGCT 7 cut(s) 10, 74, 243, 620, 664, 817, 1175
Alw21I GWGCWC 1 cut(s) 76
Alw26I GTCTC 3 cut(s) 274, 422, 1269
AlwI GGATC 1 cut(s) 689
Ama87I CYCGRG 1 cut(s) 1373
AoxI GGCC 2 cut(s) 145, 1193
ApeKI GCWGC 7 cut(s) 175, 890, 968, 1007, 1037, 1172, 1259
ApoI RAATTY 6 cut(s) 183, 193, 273, 726, 1154, 1231
AsiGI ACCGGT 1 cut(s) 979
AspLEI GCGC 1 cut(s) 331
AsuHPI GGTGA 2 cut(s) 521, 932
AvaI CYCGRG 1 cut(s) 1373
BanII GRGCYC 2 cut(s) 76, 713
Bbv12I GWGCWC 1 cut(s) 76
BbvCI CCTCAGC 1 cut(s) 1161
BbvI GCAGC 7 cut(s) 162, 877, 955, 1019, 1049, 1184, 1271
BccI CCATC 1 cut(s) 871
BceAI ACGGC 1 cut(s) 1050
BcgI CGANNNNNNTGC 2 cut(s) 891, 925
BciT130I CCWGG 2 cut(s) 915, 1342
BciVI GTATCC 1 cut(s) 1013
BcoDI GTCTC 3 cut(s) 274, 422, 1269
BfmI CTRYAG 1 cut(s) 1281
BfuI GTATCC 1 cut(s) 1013
BisI GCNGC 9 cut(s) 36, 145, 176, 891, 969, 1008, 1038, 1173, 1260
BlsI GCNGC 9 cut(s) 37, 146, 177, 892, 970, 1009, 1039, 1174, 1261
BmcAI AGTACT 1 cut(s) 505
Bme1390I CCNGG 2 cut(s) 915, 1342
BmeT110I CYCGRG 1 cut(s) 1373
BmiI GGNNCC 1 cut(s) 1078
BmrFI CCNGG 2 cut(s) 915, 1342
BmsI GCATC 8 cut(s) 192, 440, 543, 913, 980, 1033, 1321, 1345
BpmI CTGGAG 3 cut(s) 145, 852, 936
Bpu10I CCTNAGC 1 cut(s) 1161
BpuEI CTTGAG 1 cut(s) 473
BsaI GGTCTC 1 cut(s) 422
BsaJI CCNNGG 6 cut(s) 96, 913, 1098, 1144, 1341, 1372
BsaWI WCCGGW 1 cut(s) 979
Bse118I RCCGGY 1 cut(s) 979
Bse1I ACTGG 5 cut(s) 162, 219, 511, 1078, 1327
Bse3DI GCAATG 2 cut(s) 563, 984
BseBI CCWGG 2 cut(s) 915, 1342
BseDI CCNNGG 6 cut(s) 96, 913, 1098, 1144, 1341, 1372
BseMI GCAATG 2 cut(s) 563, 984
BseMII CTCAG 2 cut(s) 433, 1152
BseNI ACTGG 5 cut(s) 162, 219, 511, 1078, 1327
BseRI GAGGAG 1 cut(s) 1129
BseXI GCAGC 7 cut(s) 162, 877, 955, 1019, 1049, 1184, 1271
BsgI GTGCAG 1 cut(s) 1209
BshFI GGCC 2 cut(s) 147, 1195
BshTI ACCGGT 1 cut(s) 979
BsiHKAI GWGCWC 1 cut(s) 76
BsiHKCI CYCGRG 1 cut(s) 1373
BsiSI CCGG 1 cut(s) 980
BslFI GGGAC 1 cut(s) 1265
BsmAI GTCTC 3 cut(s) 274, 422, 1269
BsmBI CGTCTC 1 cut(s) 1269
BsmFI GGGAC 1 cut(s) 1265
BsmI GAATGC 1 cut(s) 757
BsnI GGCC 2 cut(s) 147, 1195
Bso31I GGTCTC 1 cut(s) 422
BsoBI CYCGRG 1 cut(s) 1373
Bsp1286I GDGCHC 2 cut(s) 76, 713
Bsp143I GATC 3 cut(s) 82, 681, 1271
Bsp19I CCATGG 1 cut(s) 1144
BspACI CCGC 3 cut(s) 35, 104, 144
BspANI GGCC 2 cut(s) 147, 1195
BspCNI CTCAG 2 cut(s) 432, 1153
BspLI GGNNCC 1 cut(s) 1078
BspPI GGATC 1 cut(s) 689
BspQI GCTCTTC 1 cut(s) 64
BspTNI GGTCTC 1 cut(s) 422
BsrBI CCGCTC 2 cut(s) 35, 104
BsrDI GCAATG 2 cut(s) 563, 984
BsrFI RCCGGY 1 cut(s) 979
BsrI ACTGG 5 cut(s) 162, 219, 511, 1078, 1327
BssAI RCCGGY 1 cut(s) 979
BssECI CCNNGG 6 cut(s) 96, 913, 1098, 1144, 1341, 1372
BssMI GATC 3 cut(s) 82, 681, 1271
BssT1I CCWWGG 3 cut(s) 96, 1098, 1144
Bst2UI CCWGG 2 cut(s) 915, 1342
Bst4CI ACNGT 3 cut(s) 696, 1109, 1282
Bst6I CTCTTC 2 cut(s) 64, 666
BstAPI GCANNNNNTGC 1 cut(s) 1043
BstC8I GCNNGC 4 cut(s) 60, 333, 523, 839
BstDEI CTNAG 3 cut(s) 419, 1161, 1218
BstDSI CCRYGG 1 cut(s) 1144
BstEII GGTNACC 1 cut(s) 509
BstHHI GCGC 1 cut(s) 331
BstKTI GATC 3 cut(s) 85, 684, 1274
BstMAI GTCTC 3 cut(s) 274, 422, 1269
BstMBI GATC 3 cut(s) 82, 681, 1271
BstMWI GCNNNNNNNGC 4 cut(s) 1037, 1043, 1052, 1223
BstNI CCWGG 2 cut(s) 915, 1342
BstPI GGTNACC 1 cut(s) 509
BstSCI CCNGG 2 cut(s) 913, 1340
BstSFI CTRYAG 1 cut(s) 1281
BstV1I GCAGC 7 cut(s) 162, 877, 955, 1019, 1049, 1184, 1271
BsuI GTATCC 1 cut(s) 1013
BsuRI GGCC 2 cut(s) 147, 1195
BtgI CCRYGG 1 cut(s) 1144
BtsIMutI CAGTG 2 cut(s) 169, 427
Cac8I GCNNGC 4 cut(s) 60, 333, 523, 839
CfoI GCGC 1 cut(s) 331
Cfr10I RCCGGY 1 cut(s) 979
CseI GACGC 3 cut(s) 830, 978, 1251
Csp6I GTAC 1 cut(s) 504
CspAI ACCGGT 1 cut(s) 979
CviAII CATG 5 cut(s) 133, 532, 767, 1027, 1145
CviQI GTAC 1 cut(s) 504
DdeI CTNAG 3 cut(s) 419, 1161, 1218
DpnI GATC 3 cut(s) 84, 683, 1273
DpnII GATC 3 cut(s) 82, 681, 1271
EaeI YGGCCR 1 cut(s) 145
Eam1104I CTCTTC 2 cut(s) 64, 666
EarI CTCTTC 2 cut(s) 64, 666
Ecl136II GAGCTC 1 cut(s) 74
Eco130I CCWWGG 3 cut(s) 96, 1098, 1144
Eco24I GRGCYC 2 cut(s) 76, 713
Eco31I GGTCTC 1 cut(s) 422
Eco53kI GAGCTC 1 cut(s) 74
Eco57I CTGAAG 1 cut(s) 1174
Eco88I CYCGRG 1 cut(s) 1373
Eco91I GGTNACC 1 cut(s) 509
EcoICRI GAGCTC 1 cut(s) 74
EcoO65I GGTNACC 1 cut(s) 509
EcoRI GAATTC 1 cut(s) 1231
EcoRII CCWGG 2 cut(s) 913, 1340
EcoT14I CCWWGG 3 cut(s) 96, 1098, 1144
EcoT38I GRGCYC 2 cut(s) 76, 713
ErhI CCWWGG 3 cut(s) 96, 1098, 1144
Esp3I CGTCTC 1 cut(s) 1269
FaeI CATG 5 cut(s) 136, 535, 770, 1030, 1148
FalI AAGNNNNNCTT 2 cut(s) 110, 142
FaqI GGGAC 1 cut(s) 1265
FatI CATG 5 cut(s) 132, 531, 766, 1026, 1144
FauI CCCGC 1 cut(s) 97
FauNDI CATATG 1 cut(s) 325
Fnu4HI GCNGC 9 cut(s) 36, 145, 176, 891, 969, 1008, 1038, 1173, 1260
FriOI GRGCYC 2 cut(s) 76, 713
Fsp4HI GCNGC 9 cut(s) 36, 145, 176, 891, 969, 1008, 1038, 1173, 1260
GlaI GCGC 1 cut(s) 330
GluI GCNGC 9 cut(s) 36, 145, 176, 891, 969, 1008, 1038, 1173, 1260
GsuI CTGGAG 3 cut(s) 145, 852, 936
HaeIII GGCC 2 cut(s) 147, 1195
HapII CCGG 1 cut(s) 980
HgaI GACGC 3 cut(s) 830, 978, 1251
HhaI GCGC 1 cut(s) 331
Hin1II CATG 5 cut(s) 136, 535, 770, 1030, 1148
Hin6I GCGC 1 cut(s) 329
HinP1I GCGC 1 cut(s) 329
HindIII AAGCTT 1 cut(s) 815
HinfI GANTC 3 cut(s) 151, 872, 1202
HpaII CCGG 1 cut(s) 980
HphI GGTGA 2 cut(s) 521, 932
Hpy166II GTNNAC 1 cut(s) 1366
Hpy188I TCNGA 8 cut(s) 32, 78, 279, 538, 902, 937, 1000, 1207
Hpy188III TCNNGA 8 cut(s) 52, 110, 127, 298, 374, 452, 869, 1250
Hpy8I GTNNAC 1 cut(s) 1366
Hpy99I CGWCG 1 cut(s) 18
HpyAV CCTTC 5 cut(s) 107, 635, 772, 778, 1198
HpyCH4III ACNGT 3 cut(s) 696, 1109, 1282
HpyCH4V TGCA 9 cut(s) 521, 757, 971, 1055, 1172, 1226, 1304, 1334, 1384
HpyF10VI GCNNNNNNNGC 4 cut(s) 1037, 1043, 1052, 1223
HpyF3I CTNAG 3 cut(s) 419, 1161, 1218
Hsp92II CATG 5 cut(s) 136, 535, 770, 1030, 1148
HspAI GCGC 1 cut(s) 329
Kzo9I GATC 3 cut(s) 82, 681, 1271
LguI GCTCTTC 1 cut(s) 64
LmnI GCTCC 2 cut(s) 79, 101
Lsp1109I GCAGC 7 cut(s) 162, 877, 955, 1019, 1049, 1184, 1271
LweI GCATC 8 cut(s) 192, 440, 543, 913, 980, 1033, 1321, 1345
MaeIII GTNAC 3 cut(s) 509, 720, 769
MalI GATC 3 cut(s) 84, 683, 1273
MbiI CCGCTC 2 cut(s) 35, 104
MboI GATC 3 cut(s) 82, 681, 1271
MboII GAAGA 5 cut(s) 81, 221, 551, 597, 683
MfeI CAATTG 1 cut(s) 1307
MhlI GDGCHC 2 cut(s) 76, 713
MlyI GAGTC 1 cut(s) 160
MmeI TCCRAC 1 cut(s) 925
MseI TTAA 3 cut(s) 636, 746, 1094
MslI CAYNNNNRTG 1 cut(s) 530
MspA1I CMGCKG 1 cut(s) 1175
MspI CCGG 1 cut(s) 980
MspR9I CCNGG 2 cut(s) 915, 1342
MunI CAATTG 1 cut(s) 1307
Mva1269I GAATGC 1 cut(s) 757
MvaI CCWGG 2 cut(s) 915, 1342
MwoI GCNNNNNNNGC 4 cut(s) 1037, 1043, 1052, 1223
NcoI CCATGG 1 cut(s) 1144
NdeI CATATG 1 cut(s) 325
NdeII GATC 3 cut(s) 82, 681, 1271
NlaIII CATG 5 cut(s) 136, 535, 770, 1030, 1148
NlaIV GGNNCC 1 cut(s) 1078
NmuCI GTSAC 1 cut(s) 509
PciSI GCTCTTC 1 cut(s) 64
PctI GAATGC 1 cut(s) 757
PfeI GAWTC 2 cut(s) 872, 1202
PflFI GACNNNGTC 1 cut(s) 532
PinAI ACCGGT 1 cut(s) 979
PkrI GCNGC 9 cut(s) 37, 146, 177, 892, 970, 1009, 1039, 1174, 1261
PleI GAGTC 1 cut(s) 159
PpsI GAGTC 1 cut(s) 159
Psp124BI GAGCTC 1 cut(s) 76
Psp6I CCWGG 2 cut(s) 913, 1340
PspEI GGTNACC 1 cut(s) 509
PspGI CCWGG 2 cut(s) 913, 1340
PspN4I GGNNCC 1 cut(s) 1078
PsrI GAACNNNNNNTAC 2 cut(s) 432, 464
PsyI GACNNNGTC 1 cut(s) 532
PvuII CAGCTG 1 cut(s) 1175
RsaI GTAC 1 cut(s) 505
RsaNI GTAC 1 cut(s) 504
RseI CAYNNNNRTG 1 cut(s) 530
SacI GAGCTC 1 cut(s) 76
SapI GCTCTTC 1 cut(s) 64
SaqAI TTAA 3 cut(s) 636, 746, 1094
SatI GCNGC 9 cut(s) 36, 145, 176, 891, 969, 1008, 1038, 1173, 1260
Sau3AI GATC 3 cut(s) 82, 681, 1271
ScaI AGTACT 1 cut(s) 505
SchI GAGTC 1 cut(s) 160
ScrFI CCNGG 2 cut(s) 915, 1342
SduI GDGCHC 2 cut(s) 76, 713
SfaNI GCATC 8 cut(s) 192, 440, 543, 913, 980, 1033, 1321, 1345
SfcI CTRYAG 1 cut(s) 1281
SmiMI CAYNNNNRTG 1 cut(s) 530
SmlI CTYRAG 1 cut(s) 452
SmoI CTYRAG 1 cut(s) 452
SsiI CCGC 3 cut(s) 35, 104, 144
SspI AATATT 1 cut(s) 353
SstI GAGCTC 1 cut(s) 76
StyD4I CCNGG 2 cut(s) 913, 1340
StyI CCWWGG 3 cut(s) 96, 1098, 1144
TaaI ACNGT 3 cut(s) 696, 1109, 1282
TaqI TCGA 2 cut(s) 207, 570
TatI WGTACW 1 cut(s) 503
TauI GCSGC 2 cut(s) 38, 147
TfiI GAWTC 2 cut(s) 872, 1202
Tru1I TTAA 3 cut(s) 636, 746, 1094
Tru9I TTAA 3 cut(s) 636, 746, 1094
TscAI CASTG 2 cut(s) 169, 427
TseFI GTSAC 1 cut(s) 509
TseI GCWGC 7 cut(s) 175, 890, 968, 1007, 1037, 1172, 1259
Tsp45I GTSAC 1 cut(s) 509
TspDTI ATGAA 3 cut(s) 654, 752, 1224
TspGWI ACGGA 1 cut(s) 31
TspRI CASTG 2 cut(s) 169, 427
Tth111I GACNNNGTC 1 cut(s) 532
XapI RAATTY 6 cut(s) 183, 193, 273, 726, 1154, 1231
ZrmI AGTACT 1 cut(s) 505
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.