RLG00000005008

protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0001220
Physical Location & Seq
Forward (+)
64323571 .. 64327824
4254 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000005008

Sequence Viewer

Length: 1800 bp
ATGTCGAAGCTGATTCTCCTCCACGCTTCCCCCCACCTCACCCCAATCCACACCCGAGCCTTCACCGCCGTCGTCGGAGCCTCTCTCTCCACGCCGCTCCTCCACTCTCACTGCCGTCCTCTCTTCTCTCACTCTTCTCCTCCTCTCTCTCTCCGCCGCCGCCTCTCCCTCCGCGCCTTCGACGACTCGTCGTCCGCCACCAAACCAGATGAATCGACCGAAAACGGCGCCGTTTCAGCTCCCGAGGCTAAAATCGACGCCGAGAATGCCAAAGCTAAGAAGGACAAGTACTATCCGAGTGGCGAGTTCCAGTTTCAGGAGGCGAGCCTCTGGAAGAGCTTCGTCGTTAAGCTTCGGATGCTCTTTGCTTATCCTTGGGAGCGGGTCAAGAAGGGAAGCGTCTTGACCATGACATTGCGCGGCCAGGAGATTTATGCCCCTCCAAGTGCTTATTTTTCCTTGTTTGGTCTCAGTGTTCAGGCATCATTTGTAAGAGGTGTTCTTGAGAAAATTGGAGTTGAGCCACAAGTGGAAAGGATTGGTAAATATAAAAGTGCTGGTGACCAACTTGCGCGCACGACCATGTCCGAAGAAAATTGTGAGATGCTGACAGCATTGCTCAATAACATATATGGGAACTGGCTGGACATAATTTCTTCTACAAGAGGAAAAAAGAGAGAAGATATTGAGAAGTTTGTTAATGAAGGGGTGTACCAAGTAGAAAAGCTGAAAGAAGAGGGCTGGATCACAAACATACAGTATGATGATGAGGTTACTTCAATGTTGAAGGAAAGACTTGGAGTACAAAAAGAGAAAAAGCTTCCTATGGTTGATTACAGGAAATATTCTAAGGTCAGGAAATGGACTCTTGGTTTATCAGGCGGTAAAGACAAGATAGCCATAATAAGAGCCTCAGGCAGTATTAGTCGTGTTCGAGGTTCTTTTAGTCTACCTGGATCATCTATTGTTGGAGAACAGTTCATTGAGAAGATCCGTACTATAAGAGAATCAAAAAGATACAAGGCTGCTATTATCCGAATTGACAGCCCTGGAGGTGATGCCCTTGCTTCTGATTTGATGTGGAGGGAAATCAAACTTTTGGCTGCATCAAAACCGGTCATTGCATCAATGTCTGATGTGGCAGCAAGTGGAGGATACTATATGGCTATGGCAGCAGATGCCATTGTTGCAGAAAATCTAACCTTAACTGGTTCCATTGGAGTTGTCACAGGGAAGTTTAACCTGGGAAAACTGTATGAGAAGATTGGCTTCAACAAGGAAATCATATCAAGGGGGAAATTTGCAGAGGTTCTTGCAGCCGAACAACGACCCTTCAAACCAGAGGAAGCTGAACTCTTTGCCAAGTCTGCACAGAATTCGTATAAGCAATTTCGGGACAAGGCAGCTTCTTCCAGATCAATGACTGTAGATAAAATGGAGGAAGTTGCACAAGGGAGAGTTTGGGCTGGTAAAGATGCAGCTTCGAGGGGTTTAGTTGATGCCATTGGTGGACTTTCTCGGGCTGTTGCAATAGCAAAGCTCAAGGCAAATATACCACAAGACACAGAGGTTACACTTGTGGAGCTTGCAAGACCCTCCCCTTCACTGCCAGAACTTTTAAGTGGCGTAGGGAGTACTCTGGTTGGAGTCGAGCAAACGATGAAGACACTGTTGCAAGACTTAACATTTTCTGATGGAGTTCAAGCCCGGATGGATGGAATCTTATTTCAGAGACTGGAGGGAGCTTCTAATGCCAACCCCATCTTCAATTTGATAAGAGATTACCTTGGTTCCCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_S49

Protein Analysis

600

Amino Acids

65.92

Weight (kDa)

9.39

Isoelectric Point (pI)

36.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S49 PF01343 141 - 268 8e-17 Peptidase family S49
Peptidase_S49 PF01343 367 - 517 2.3e-42 Peptidase family S49
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 227
AccBSI CCGCTC 2 cut(s) 97, 382
AccI GTMKAC 1 cut(s) 949
AccII CGCG 3 cut(s) 174, 420, 574
AclWI GGATC 3 cut(s) 752, 964, 985
AcoI YGGCCR 1 cut(s) 421
AcsI RAATTY 2 cut(s) 1298, 1375
AcyI GRCGYC 2 cut(s) 228, 258
AfaI GTAC 5 cut(s) 290, 713, 804, 997, 1636
AfiI CCNNNNNNNGG 1 cut(s) 316
AgeI ACCGGT 1 cut(s) 1114
AgsI TTSAA 6 cut(s) 780, 787, 1273, 1336, 1703, 1768
AhdI GACNNNNNGTC 2 cut(s) 187, 190
AjnI CCWGG 4 cut(s) 423, 952, 1048, 1242
AjuI GAANNNNNNNTTGG 2 cut(s) 1748, 1780
Alw26I GTCTC 2 cut(s) 473, 1726
AlwI GGATC 3 cut(s) 752, 964, 985
AlwNI CAGNNNCTG 1 cut(s) 1735
Ama87I CYCGRG 3 cut(s) 54, 242, 1518
AoxI GGCC 1 cut(s) 421
ApeKI GCWGC 7 cut(s) 1025, 1103, 1142, 1172, 1316, 1403, 1478
ApoI RAATTY 2 cut(s) 1298, 1375
AsiGI ACCGGT 1 cut(s) 1114
Asp700I GAANNNNTTC 1 cut(s) 338
AspLEI GCGC 5 cut(s) 176, 230, 420, 574, 576
AsuC2I CCSGG 1 cut(s) 1708
AsuHPI GGTGA 4 cut(s) 31, 55, 572, 1067
AvaI CYCGRG 3 cut(s) 54, 242, 1518
AxyI CCTNAGG 1 cut(s) 913
BanI GGYRCC 1 cut(s) 227
BarI GAAGNNNNNNTAC 2 cut(s) 272, 304
BbsI GAAGAC 1 cut(s) 1670
BbvI GCAGC 7 cut(s) 1012, 1090, 1154, 1184, 1328, 1415, 1490
BccI CCATC 4 cut(s) 1688, 1705, 1709, 1769
BceAI ACGGC 4 cut(s) 53, 99, 215, 241
BcgI CGANNNNNNTGC 2 cut(s) 1359, 1393
BciT130I CCWGG 4 cut(s) 425, 954, 1050, 1244
BciVI GTATCC 1 cut(s) 1148
BcnI CCSGG 1 cut(s) 1708
BcoDI GTCTC 2 cut(s) 473, 1726
BfmI CTRYAG 1 cut(s) 1425
BfoI RGCGCY 1 cut(s) 231
BfuI GTATCC 1 cut(s) 1148
BmcAI AGTACT 2 cut(s) 290, 1636
Bme1390I CCNGG 5 cut(s) 425, 954, 1050, 1244, 1708
BmeRI GACNNNNNGTC 2 cut(s) 187, 190
BmeT110I CYCGRG 3 cut(s) 54, 242, 1518
BmiI GGNNCC 4 cut(s) 79, 229, 1213, 1792
BmrFI CCNGG 5 cut(s) 425, 954, 1050, 1244, 1708
BmsI GCATC 9 cut(s) 348, 491, 594, 1048, 1115, 1133, 1168, 1465, 1489
BpiI GAAGAC 1 cut(s) 1670
BplI GAGNNNNNCTC 2 cut(s) 69, 101
BpmI CTGGAG 2 cut(s) 1071, 1757
BpuEI CTTGAG 2 cut(s) 524, 1526
BpuMI CCSGG 1 cut(s) 1708
BsaHI GRCGYC 2 cut(s) 228, 258
BsaI GGTCTC 1 cut(s) 473
BsaJI CCNNGG 5 cut(s) 243, 374, 1048, 1243, 1786
BsaWI WCCGGW 1 cut(s) 1114
Bsc4I CCNNNNNNNGG 1 cut(s) 316
Bse118I RCCGGY 1 cut(s) 1114
Bse1I ACTGG 4 cut(s) 310, 644, 1213, 1740
Bse21I CCTNAGG 1 cut(s) 913
Bse3DI GCAATG 3 cut(s) 413, 614, 1119
BseBI CCWGG 4 cut(s) 425, 954, 1050, 1244
BseDI CCNNGG 5 cut(s) 243, 374, 1048, 1243, 1786
BseGI GGATG 3 cut(s) 363, 1716, 1720
BseLI CCNNNNNNNGG 1 cut(s) 316
BseMI GCAATG 3 cut(s) 413, 614, 1119
BseMII CTCAG 2 cut(s) 484, 927
BseNI ACTGG 4 cut(s) 310, 644, 1213, 1740
BsePI GCGCGC 1 cut(s) 572
BseRI GAGGAG 4 cut(s) 8, 89, 129, 132
BseXI GCAGC 7 cut(s) 1012, 1090, 1154, 1184, 1328, 1415, 1490
BsgI GTGCAG 1 cut(s) 1353
Bsh1236I CGCG 3 cut(s) 174, 420, 574
Bsh1285I CGRYCG 1 cut(s) 219
BshFI GGCC 1 cut(s) 423
BshNI GGYRCC 1 cut(s) 227
BshTI ACCGGT 1 cut(s) 1114
BsiEI CGRYCG 1 cut(s) 219
BsiHKCI CYCGRG 3 cut(s) 54, 242, 1518
BsiSI CCGG 2 cut(s) 1115, 1708
BslFI GGGAC 1 cut(s) 1409
BslI CCNNNNNNNGG 1 cut(s) 316
BsmAI GTCTC 2 cut(s) 473, 1726
BsmFI GGGAC 1 cut(s) 1409
BsmI GAATGC 1 cut(s) 271
BsnI GGCC 1 cut(s) 423
Bso31I GGTCTC 1 cut(s) 473
BsoBI CYCGRG 3 cut(s) 54, 242, 1518
Bsp143I GATC 4 cut(s) 744, 956, 990, 1415
BspANI GGCC 1 cut(s) 423
BspCNI CTCAG 2 cut(s) 483, 926
BspFNI CGCG 3 cut(s) 174, 420, 574
BspLI GGNNCC 4 cut(s) 79, 229, 1213, 1792
BspPI GGATC 3 cut(s) 752, 964, 985
BspQI GCTCTTC 1 cut(s) 329
BspT107I GGYRCC 1 cut(s) 227
BspTNI GGTCTC 1 cut(s) 473
BsrBI CCGCTC 2 cut(s) 97, 382
BsrDI GCAATG 3 cut(s) 413, 614, 1119
BsrFI RCCGGY 1 cut(s) 1114
BsrI ACTGG 4 cut(s) 310, 644, 1213, 1740
BssAI RCCGGY 1 cut(s) 1114
BssECI CCNNGG 5 cut(s) 243, 374, 1048, 1243, 1786
BssHII GCGCGC 1 cut(s) 572
BssMI GATC 4 cut(s) 744, 956, 990, 1415
BssNI GRCGYC 2 cut(s) 228, 258
BssT1I CCWWGG 2 cut(s) 374, 1786
Bst2UI CCWGG 4 cut(s) 425, 954, 1050, 1244
Bst4CI ACNGT 5 cut(s) 759, 978, 1254, 1426, 1671
Bst6I CTCTTC 4 cut(s) 128, 139, 329, 729
BstACI GRCGYC 2 cut(s) 228, 258
BstAPI GCANNNNNTGC 1 cut(s) 1178
BstC8I GCNNGC 3 cut(s) 325, 574, 1587
BstDEI CTNAG 4 cut(s) 276, 470, 849, 913
BstEII GGTNACC 1 cut(s) 560
BstF5I GGATG 3 cut(s) 363, 1716, 1720
BstFNI CGCG 3 cut(s) 174, 420, 574
BstH2I RGCGCY 1 cut(s) 231
BstHHI GCGC 5 cut(s) 176, 230, 420, 574, 576
BstKTI GATC 4 cut(s) 747, 959, 993, 1418
BstMAI GTCTC 2 cut(s) 473, 1726
BstMBI GATC 4 cut(s) 744, 956, 990, 1415
BstMCI CGRYCG 1 cut(s) 219
BstNI CCWGG 4 cut(s) 425, 954, 1050, 1244
BstPI GGTNACC 1 cut(s) 560
BstSCI CCNGG 5 cut(s) 423, 952, 1048, 1242, 1706
BstSFI CTRYAG 1 cut(s) 1425
BstUI CGCG 3 cut(s) 174, 420, 574
BstV1I GCAGC 7 cut(s) 1012, 1090, 1154, 1184, 1328, 1415, 1490
BstV2I GAAGAC 1 cut(s) 1670
BstX2I RGATCY 1 cut(s) 990
BstYI RGATCY 1 cut(s) 990
Bsu36I CCTNAGG 1 cut(s) 913
BsuI GTATCC 1 cut(s) 1148
BsuRI GGCC 1 cut(s) 423
BtsCI GGATG 3 cut(s) 363, 1716, 1720
BtsI GCAGTG 2 cut(s) 109, 1604
BtsIMutI CAGTG 4 cut(s) 109, 478, 1604, 1667
Cac8I GCNNGC 3 cut(s) 325, 574, 1587
CaiI CAGNNNCTG 1 cut(s) 1735
CfoI GCGC 5 cut(s) 176, 230, 420, 574, 576
Cfr10I RCCGGY 1 cut(s) 1114
CseI GACGC 2 cut(s) 266, 388
Csp6I GTAC 5 cut(s) 289, 712, 803, 996, 1635
CspAI ACCGGT 1 cut(s) 1114
CviAII CATG 2 cut(s) 409, 583
CviQI GTAC 5 cut(s) 289, 712, 803, 996, 1635
DdeI CTNAG 4 cut(s) 276, 470, 849, 913
DinI GGCGCC 1 cut(s) 229
DpnI GATC 4 cut(s) 746, 958, 992, 1417
DpnII GATC 4 cut(s) 744, 956, 990, 1415
DriI GACNNNNNGTC 2 cut(s) 187, 190
EaeI YGGCCR 1 cut(s) 421
Eam1104I CTCTTC 4 cut(s) 128, 139, 329, 729
Eam1105I GACNNNNNGTC 2 cut(s) 187, 190
EarI CTCTTC 4 cut(s) 128, 139, 329, 729
EciI GGCGGA 2 cut(s) 143, 184
Eco130I CCWWGG 2 cut(s) 374, 1786
Eco31I GGTCTC 1 cut(s) 473
Eco81I CCTNAGG 1 cut(s) 913
Eco88I CYCGRG 3 cut(s) 54, 242, 1518
Eco91I GGTNACC 1 cut(s) 560
EcoO65I GGTNACC 1 cut(s) 560
EcoRI GAATTC 1 cut(s) 1375
EcoRII CCWGG 4 cut(s) 423, 952, 1048, 1242
EcoT14I CCWWGG 2 cut(s) 374, 1786
EgeI GGCGCC 1 cut(s) 229
EheI GGCGCC 1 cut(s) 229
ErhI CCWWGG 2 cut(s) 374, 1786
FaeI CATG 2 cut(s) 412, 586
FalI AAGNNNNNCTT 2 cut(s) 1253, 1285
FaqI GGGAC 1 cut(s) 1409
FatI CATG 2 cut(s) 408, 582
FauI CCCGC 1 cut(s) 375
FblI GTMKAC 1 cut(s) 949
FokI GGATG 3 cut(s) 370, 1723, 1727
GlaI GCGC 5 cut(s) 175, 229, 419, 573, 575
GsuI CTGGAG 2 cut(s) 1071, 1757
HaeII RGCGCY 1 cut(s) 231
HaeIII GGCC 1 cut(s) 423
HapII CCGG 2 cut(s) 1115, 1708
HgaI GACGC 2 cut(s) 266, 388
HhaI GCGC 5 cut(s) 176, 230, 420, 574, 576
Hin1I GRCGYC 2 cut(s) 228, 258
Hin1II CATG 2 cut(s) 412, 586
Hin6I GCGC 5 cut(s) 174, 228, 418, 572, 574
HinP1I GCGC 5 cut(s) 174, 228, 418, 572, 574
HindIII AAGCTT 2 cut(s) 350, 818
HinfI GANTC 7 cut(s) 13, 185, 212, 865, 1007, 1647, 1719
HpaII CCGG 2 cut(s) 1115, 1708
HphI GGTGA 4 cut(s) 31, 55, 572, 1067
Hpy166II GTNNAC 3 cut(s) 712, 950, 1511
Hpy188I TCNGA 9 cut(s) 77, 297, 357, 589, 1037, 1072, 1135, 1693, 1731
Hpy188III TCNNGA 9 cut(s) 242, 317, 331, 388, 403, 503, 856, 1394, 1413
Hpy8I GTNNAC 3 cut(s) 712, 950, 1511
Hpy99I CGWCG 6 cut(s) 74, 77, 185, 193, 260, 347
HpyAV CCTTC 8 cut(s) 70, 187, 274, 385, 698, 781, 1342, 1611
HpyCH4III ACNGT 5 cut(s) 759, 978, 1254, 1426, 1671
HpyF3I CTNAG 4 cut(s) 276, 470, 849, 913
Hsp92I GRCGYC 2 cut(s) 228, 258
Hsp92II CATG 2 cut(s) 412, 586
HspAI GCGC 5 cut(s) 174, 228, 418, 572, 574
KasI GGCGCC 1 cut(s) 227
Kzo9I GATC 4 cut(s) 744, 956, 990, 1415
LguI GCTCTTC 1 cut(s) 329
LmnI GCTCC 6 cut(s) 77, 102, 244, 379, 1582, 1742
Lsp1109I GCAGC 7 cut(s) 1012, 1090, 1154, 1184, 1328, 1415, 1490
LweI GCATC 9 cut(s) 348, 491, 594, 1048, 1115, 1133, 1168, 1465, 1489
MaeIII GTNAC 4 cut(s) 560, 772, 1225, 1570
MalI GATC 4 cut(s) 746, 958, 992, 1417
MbiI CCGCTC 2 cut(s) 97, 382
MboI GATC 4 cut(s) 744, 956, 990, 1415
MflI RGATCY 1 cut(s) 990
MluCI AATT 8 cut(s) 510, 595, 651, 1038, 1298, 1375, 1388, 1768
Mly113I GGCGCC 1 cut(s) 228
MlyI GAGTC 3 cut(s) 179, 859, 1656
MmeI TCCRAC 3 cut(s) 55, 949, 1624
MroXI GAANNNNTTC 1 cut(s) 338
MseI TTAA 6 cut(s) 348, 699, 1205, 1239, 1619, 1682
MslI CAYNNNNRTG 1 cut(s) 581
MspI CCGG 2 cut(s) 1115, 1708
MspR9I CCNGG 5 cut(s) 425, 954, 1050, 1244, 1708
Mva1269I GAATGC 1 cut(s) 271
MvaI CCWGG 4 cut(s) 425, 954, 1050, 1244
MvnI CGCG 3 cut(s) 174, 420, 574
NarI GGCGCC 1 cut(s) 228
NciI CCSGG 1 cut(s) 1708
NdeII GATC 4 cut(s) 744, 956, 990, 1415
NlaIII CATG 2 cut(s) 412, 586
NlaIV GGNNCC 4 cut(s) 79, 229, 1213, 1792
NmeAIII GCCGAG 1 cut(s) 286
NmuCI GTSAC 2 cut(s) 560, 1225
PauI GCGCGC 1 cut(s) 572
PciSI GCTCTTC 1 cut(s) 329
PctI GAATGC 1 cut(s) 271
PdmI GAANNNNTTC 1 cut(s) 338
PfeI GAWTC 4 cut(s) 13, 212, 1007, 1719
PflFI GACNNNGTC 1 cut(s) 583
PinAI ACCGGT 1 cut(s) 1114
PleI GAGTC 3 cut(s) 179, 859, 1655
PluTI GGCGCC 1 cut(s) 231
PpsI GAGTC 3 cut(s) 179, 859, 1655
Psp6I CCWGG 4 cut(s) 423, 952, 1048, 1242
PspEI GGTNACC 1 cut(s) 560
PspGI CCWGG 4 cut(s) 423, 952, 1048, 1242
PspN4I GGNNCC 4 cut(s) 79, 229, 1213, 1792
PsrI GAACNNNNNNTAC 2 cut(s) 483, 515
PstNI CAGNNNCTG 1 cut(s) 1735
PsuI RGATCY 1 cut(s) 990
PsyI GACNNNGTC 1 cut(s) 583
PteI GCGCGC 1 cut(s) 572
RsaI GTAC 5 cut(s) 290, 713, 804, 997, 1636
RsaNI GTAC 5 cut(s) 289, 712, 803, 996, 1635
RseI CAYNNNNRTG 1 cut(s) 581
SapI GCTCTTC 1 cut(s) 329
SaqAI TTAA 6 cut(s) 348, 699, 1205, 1239, 1619, 1682
Sau3AI GATC 4 cut(s) 744, 956, 990, 1415
ScaI AGTACT 2 cut(s) 290, 1636
SchI GAGTC 3 cut(s) 179, 859, 1656
ScrFI CCNGG 5 cut(s) 425, 954, 1050, 1244, 1708
SfaNI GCATC 9 cut(s) 348, 491, 594, 1048, 1115, 1133, 1168, 1465, 1489
SfcI CTRYAG 1 cut(s) 1425
SfoI GGCGCC 1 cut(s) 229
SmiMI CAYNNNNRTG 1 cut(s) 581
SmlI CTYRAG 2 cut(s) 503, 1541
SmoI CTYRAG 2 cut(s) 503, 1541
Sse9I AATT 8 cut(s) 510, 595, 651, 1038, 1298, 1375, 1388, 1768
SspDI GGCGCC 1 cut(s) 227
SspI AATATT 1 cut(s) 845
StyD4I CCNGG 5 cut(s) 423, 952, 1048, 1242, 1706
StyI CCWWGG 2 cut(s) 374, 1786
TaaI ACNGT 5 cut(s) 759, 978, 1254, 1426, 1671
TaqI TCGA 7 cut(s) 5, 180, 215, 255, 934, 1484, 1650
TaqII GACCGA 1 cut(s) 233
TasI AATT 8 cut(s) 510, 595, 651, 1038, 1298, 1375, 1388, 1768
TatI WGTACW 3 cut(s) 288, 802, 1634
TauI GCSGC 4 cut(s) 97, 159, 162, 423
TfiI GAWTC 4 cut(s) 13, 212, 1007, 1719
Tru1I TTAA 6 cut(s) 348, 699, 1205, 1239, 1619, 1682
Tru9I TTAA 6 cut(s) 348, 699, 1205, 1239, 1619, 1682
TscAI CASTG 4 cut(s) 116, 478, 1611, 1674
TseFI GTSAC 2 cut(s) 560, 1225
TseI GCWGC 7 cut(s) 1025, 1103, 1142, 1172, 1316, 1403, 1478
Tsp45I GTSAC 2 cut(s) 560, 1225
TspDTI ATGAA 4 cut(s) 225, 717, 970, 1676
TspGWI ACGGA 1 cut(s) 983
TspRI CASTG 4 cut(s) 116, 478, 1611, 1674
Tth111I GACNNNGTC 1 cut(s) 583
XapI RAATTY 2 cut(s) 1298, 1375
XmiI GTMKAC 1 cut(s) 949
XmnI GAANNNNTTC 1 cut(s) 338
ZrmI AGTACT 2 cut(s) 290, 1636
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.