RchiOBHm_Chr7g0184031

protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0001220
Physical Location & Seq
Reverse (-)
4756541 .. 4760339
3799 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ16419

Sequence Viewer

Length: 1404 bp
ATGCTTTTTGCTTATCCTTGGGAGCGGGTTAAGAAGGGAACCGTCTTGACCATGACATTGCGAGGCCAGTTAAAGAGTCAGTTCTCTAGTGGATTATCGCTGCCTCAAATTTGTGAGAATTTTGTGAAAGTTGCATATGATCCTCGAATTGCTAGTGTGTATCTTCAAATTGAGAGCTTGAACTATGGTTGGGGTAAGTTTGTTGTGGCATATGCACCTGCTTGTAGTGAAAAAGAGTATTACCTTGCTTCTGCTTGTCAAGAGATTTTATGCCCCTCCAAGTGCTTATTTTTCTTTGTTTGGTCTCAGCTTTCAGGCATCATTTGCATTCTTGAGAAAATTGGAGTTGAGCCACAAGTGGAGAGGATTGGTAAATATAGAAGTGCTGGTGACCAACTTGCGTGCACGACCATGTCCGAAAAAAATTGTGAGATGCTGACAGCATTGCTCGATAACATATATGGGAACTGGCTGGACATAATTTCTTCTACAAGAGGAAAAAAGAGAGAAGATATTGAGAAGTTTGTTAATGAAGGGGTGTACCAAGTAGAAAAGCTGAAAGAAGAGGGCTGGATCACAAACATACAGTATGATGATGAGGTTACTTCAATGTTGAAGGAAAGACTTGGAGTACAAAAAGAGAAAAAGCTTCCTATGGTTGATTACAGGAAATATTCTAAGGTCCGGAAATGGACTCTTGGTTTATCAGGCGGTAAAGACAAGATAGCCATAATAAGAGCCTCAGGCAGTATTAGTCGTGTACGAGGTTCTTTTAGTCTACCCGGATCATCTATTGTTGGAGAACAGTTCATTGAGAAGTTCCGTAGTATAAGAGGTATTGTGAATCAAAAAGATACAAAGGCTGCTATTATCCGAATTGACAGCCCTGGAGGTGATGCCCTTGCTTCTGATTTGATGTGGAGGGAAATCAAACTTTTGGCTGCATCAAAACCGGTCATTGCATCAATGTCTGACGTGGCAGCAAGTGGAGGATACTATATGGCTATGGCAGCAGATGCCATTGTTGCAGAAAGTCTAACCTTAACTGGTTCCATTGGAGTTGTCATAGGGAAGTTTAACCTTGGAAAACTGTATGAGAAGATTGCATTCAACAAAGAAATTATATCACGGGGAAAATTTGCGGAGGTTCTTGCAGCTGAACAACGACCCTTCAGGTGCACTGTCTTGTTGCATACTCTATTTTGTCTGATTTCTTTTATAATCCTGAAAGGAGACAAAATTATTAGTTCTCTTTTTCTTCCTAATTCAGACCAGAGGAAGTTTGCTAAGTCTGCACAGAATTCGTATAAGCAATTTCGGGACAAGGCAGCTTCTTCCAGATCAATGACTGTATGTTCCAATTGTTGGAGAGGAAAGATCACACATTGGAAAAGTGACAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_S49

Protein Analysis

467

Amino Acids

52.29

Weight (kDa)

9.34

Isoelectric Point (pI)

35.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S49 PF01343 66 - 211 9.2e-13 Peptidase family S49
Peptidase_S49 PF01343 313 - 386 1.7e-21 Peptidase family S49
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1220
AarI CACCTGC 1 cut(s) 226
Acc36I ACCTGC 1 cut(s) 226
AccB7I CCANNNNNTGG 1 cut(s) 1365
AccBSI CCGCTC 1 cut(s) 25
AccI GTMKAC 1 cut(s) 778
AccIII TCCGGA 1 cut(s) 684
AciI CCGC 3 cut(s) 25, 711, 1142
AclWI GGATC 3 cut(s) 134, 581, 793
AcsI RAATTY 4 cut(s) 108, 118, 1136, 1300
AcuI CTGAAG 1 cut(s) 1156
AfaI GTAC 3 cut(s) 542, 633, 762
AfiI CCNNNNNNNGG 1 cut(s) 1365
AgeI ACCGGT 1 cut(s) 952
AgsI TTSAA 5 cut(s) 167, 181, 609, 616, 1111
AjiI CACGTC 1 cut(s) 976
AjnI CCWGG 1 cut(s) 886
AluBI AGCT 6 cut(s) 177, 310, 556, 649, 1157, 1331
AluI AGCT 6 cut(s) 177, 310, 556, 649, 1157, 1331
Alw21I GWGCWC 2 cut(s) 407, 1181
Alw26I GTCTC 2 cut(s) 309, 1227
Alw44I GTGCAC 2 cut(s) 403, 1177
AlwI GGATC 3 cut(s) 134, 581, 793
Aor13HI TCCGGA 1 cut(s) 684
AoxI GGCC 1 cut(s) 64
ApaLI GTGCAC 2 cut(s) 403, 1177
ApeKI GCWGC 7 cut(s) 100, 863, 941, 980, 1010, 1154, 1328
ApoI RAATTY 4 cut(s) 108, 118, 1136, 1300
AsiGI ACCGGT 1 cut(s) 952
AspS9I GGNCC 1 cut(s) 682
AsuC2I CCSGG 1 cut(s) 783
AsuHPI GGTGA 2 cut(s) 401, 905
AvaII GGWCC 1 cut(s) 682
AxyI CCTNAGG 1 cut(s) 742
BaeGI GKGCMC 2 cut(s) 407, 1181
Bbv12I GWGCWC 2 cut(s) 407, 1181
BbvI GCAGC 7 cut(s) 87, 850, 928, 992, 1022, 1166, 1340
BcgI CGANNNNNNTGC 2 cut(s) 1284, 1318
BciT130I CCWGG 1 cut(s) 888
BciVI GTATCC 1 cut(s) 986
BcnI CCSGG 1 cut(s) 783
BcoDI GTCTC 2 cut(s) 309, 1227
BfaI CTAG 2 cut(s) 87, 153
BfuAI ACCTGC 1 cut(s) 226
BfuI GTATCC 1 cut(s) 986
BisI GCNGC 7 cut(s) 101, 864, 942, 981, 1011, 1155, 1329
BlsI GCNGC 7 cut(s) 102, 865, 943, 982, 1012, 1156, 1330
Bme1390I CCNGG 2 cut(s) 783, 888
Bme18I GGWCC 1 cut(s) 682
BmgBI CACGTC 1 cut(s) 976
BmgT120I GGNCC 1 cut(s) 682
BmiI GGNNCC 2 cut(s) 40, 1051
BmrFI CCNGG 2 cut(s) 783, 888
BmsI GCATC 6 cut(s) 327, 423, 886, 953, 971, 1006
BpmI CTGGAG 1 cut(s) 909
BpuEI CTTGAG 1 cut(s) 353
BpuMI CCSGG 1 cut(s) 783
BsaI GGTCTC 1 cut(s) 309
BsaJI CCNNGG 3 cut(s) 17, 886, 1081
BsaWI WCCGGW 2 cut(s) 684, 952
Bsc4I CCNNNNNNNGG 1 cut(s) 1365
Bse118I RCCGGY 1 cut(s) 952
Bse1I ACTGG 3 cut(s) 67, 473, 1051
Bse21I CCTNAGG 1 cut(s) 742
Bse3DI GCAATG 3 cut(s) 56, 443, 957
BseAI TCCGGA 1 cut(s) 684
BseBI CCWGG 1 cut(s) 888
BseDI CCNNGG 3 cut(s) 17, 886, 1081
BseLI CCNNNNNNNGG 1 cut(s) 1365
BseMI GCAATG 3 cut(s) 56, 443, 957
BseMII CTCAG 2 cut(s) 320, 756
BseNI ACTGG 3 cut(s) 67, 473, 1051
BseSI GKGCMC 2 cut(s) 407, 1181
BseXI GCAGC 7 cut(s) 87, 850, 928, 992, 1022, 1166, 1340
BsgI GTGCAG 1 cut(s) 1278
BshFI GGCC 1 cut(s) 66
BshTI ACCGGT 1 cut(s) 952
BsiHKAI GWGCWC 2 cut(s) 407, 1181
BsiSI CCGG 3 cut(s) 685, 783, 953
BslFI GGGAC 1 cut(s) 1334
BslI CCNNNNNNNGG 1 cut(s) 1365
BsmAI GTCTC 2 cut(s) 309, 1227
BsmFI GGGAC 1 cut(s) 1334
BsmI GAATGC 2 cut(s) 327, 1106
BsnI GGCC 1 cut(s) 66
Bso31I GGTCTC 1 cut(s) 309
Bsp1286I GDGCHC 2 cut(s) 407, 1181
Bsp13I TCCGGA 1 cut(s) 684
Bsp143I GATC 5 cut(s) 139, 573, 785, 1340, 1377
BspACI CCGC 3 cut(s) 25, 711, 1142
BspANI GGCC 1 cut(s) 66
BspCNI CTCAG 2 cut(s) 319, 755
BspEI TCCGGA 1 cut(s) 684
BspLI GGNNCC 2 cut(s) 40, 1051
BspMI ACCTGC 1 cut(s) 226
BspPI GGATC 3 cut(s) 134, 581, 793
BspTNI GGTCTC 1 cut(s) 309
BsrBI CCGCTC 1 cut(s) 25
BsrDI GCAATG 3 cut(s) 56, 443, 957
BsrFI RCCGGY 1 cut(s) 952
BsrI ACTGG 3 cut(s) 67, 473, 1051
BssAI RCCGGY 1 cut(s) 952
BssECI CCNNGG 3 cut(s) 17, 886, 1081
BssMI GATC 5 cut(s) 139, 573, 785, 1340, 1377
BssT1I CCWWGG 2 cut(s) 17, 1081
Bst2UI CCWGG 1 cut(s) 888
Bst4CI ACNGT 6 cut(s) 43, 588, 807, 1092, 1183, 1351
Bst6I CTCTTC 1 cut(s) 558
BstAPI GCANNNNNTGC 2 cut(s) 324, 1016
BstC8I GCNNGC 1 cut(s) 403
BstDEI CTNAG 4 cut(s) 306, 678, 742, 1287
BstEII GGTNACC 1 cut(s) 389
BstKTI GATC 5 cut(s) 142, 576, 788, 1343, 1380
BstMAI GTCTC 2 cut(s) 309, 1227
BstMBI GATC 5 cut(s) 139, 573, 785, 1340, 1377
BstMWI GCNNNNNNNGC 5 cut(s) 324, 1010, 1016, 1025, 1292
BstNI CCWGG 1 cut(s) 888
BstPI GGTNACC 1 cut(s) 389
BstSCI CCNGG 2 cut(s) 781, 886
BstSLI GKGCMC 2 cut(s) 407, 1181
BstV1I GCAGC 7 cut(s) 87, 850, 928, 992, 1022, 1166, 1340
Bsu36I CCTNAGG 1 cut(s) 742
BsuI GTATCC 1 cut(s) 986
BsuRI GGCC 1 cut(s) 66
BtrI CACGTC 1 cut(s) 976
BtsIMutI CAGTG 1 cut(s) 1179
BveI ACCTGC 1 cut(s) 226
Cac8I GCNNGC 1 cut(s) 403
Cfr10I RCCGGY 1 cut(s) 952
Cfr13I GGNCC 1 cut(s) 682
Csp6I GTAC 3 cut(s) 541, 632, 761
CspAI ACCGGT 1 cut(s) 952
CviAII CATG 2 cut(s) 52, 412
CviQI GTAC 3 cut(s) 541, 632, 761
DdeI CTNAG 4 cut(s) 306, 678, 742, 1287
DpnI GATC 5 cut(s) 141, 575, 787, 1342, 1379
DpnII GATC 5 cut(s) 139, 573, 785, 1340, 1377
Eam1104I CTCTTC 1 cut(s) 558
EarI CTCTTC 1 cut(s) 558
Eco130I CCWWGG 2 cut(s) 17, 1081
Eco31I GGTCTC 1 cut(s) 309
Eco47I GGWCC 1 cut(s) 682
Eco57I CTGAAG 1 cut(s) 1156
Eco81I CCTNAGG 1 cut(s) 742
Eco91I GGTNACC 1 cut(s) 389
EcoO65I GGTNACC 1 cut(s) 389
EcoRI GAATTC 1 cut(s) 1300
EcoRII CCWGG 1 cut(s) 886
EcoT14I CCWWGG 2 cut(s) 17, 1081
ErhI CCWWGG 2 cut(s) 17, 1081
FaeI CATG 2 cut(s) 55, 415
FaqI GGGAC 1 cut(s) 1334
FatI CATG 2 cut(s) 51, 411
FauI CCCGC 1 cut(s) 18
FauNDI CATATG 2 cut(s) 136, 211
FblI GTMKAC 1 cut(s) 778
Fnu4HI GCNGC 7 cut(s) 101, 864, 942, 981, 1011, 1155, 1329
Fsp4HI GCNGC 7 cut(s) 101, 864, 942, 981, 1011, 1155, 1329
FspBI CTAG 2 cut(s) 87, 153
GluI GCNGC 7 cut(s) 101, 864, 942, 981, 1011, 1155, 1329
GsuI CTGGAG 1 cut(s) 909
HaeIII GGCC 1 cut(s) 66
HapII CCGG 3 cut(s) 685, 783, 953
Hin1II CATG 2 cut(s) 55, 415
HindIII AAGCTT 1 cut(s) 647
HinfI GANTC 3 cut(s) 76, 694, 844
HpaII CCGG 3 cut(s) 685, 783, 953
HphI GGTGA 2 cut(s) 401, 905
Hpy166II GTNNAC 5 cut(s) 405, 541, 761, 779, 1179
Hpy188I TCNGA 6 cut(s) 418, 875, 910, 973, 1209, 1270
Hpy188III TCNNGA 7 cut(s) 46, 260, 332, 685, 1225, 1319, 1338
Hpy8I GTNNAC 5 cut(s) 405, 541, 761, 779, 1179
HpyAV CCTTC 4 cut(s) 28, 527, 610, 1180
HpyCH4III ACNGT 6 cut(s) 43, 588, 807, 1092, 1183, 1351
HpyCH4IV ACGT 1 cut(s) 975
HpyF10VI GCNNNNNNNGC 5 cut(s) 324, 1010, 1016, 1025, 1292
HpyF3I CTNAG 4 cut(s) 306, 678, 742, 1287
HpySE526I ACGT 1 cut(s) 975
Hsp92II CATG 2 cut(s) 55, 415
Kpn2I TCCGGA 1 cut(s) 684
Kzo9I GATC 5 cut(s) 139, 573, 785, 1340, 1377
LmnI GCTCC 1 cut(s) 22
Lsp1109I GCAGC 7 cut(s) 87, 850, 928, 992, 1022, 1166, 1340
LweI GCATC 6 cut(s) 327, 423, 886, 953, 971, 1006
MaeI CTAG 2 cut(s) 87, 153
MaeII ACGT 1 cut(s) 975
MaeIII GTNAC 3 cut(s) 389, 601, 1394
MalI GATC 5 cut(s) 141, 575, 787, 1342, 1379
MbiI CCGCTC 1 cut(s) 25
MboI GATC 5 cut(s) 139, 573, 785, 1340, 1377
MboII GAAGA 7 cut(s) 155, 477, 521, 575, 1111, 1250, 1326
MfeI CAATTG 1 cut(s) 1360
MhlI GDGCHC 2 cut(s) 407, 1181
MlyI GAGTC 2 cut(s) 85, 688
MmeI TCCRAC 2 cut(s) 778, 1346
MroI TCCGGA 1 cut(s) 684
MseI TTAA 5 cut(s) 30, 71, 528, 1043, 1077
MslI CAYNNNNRTG 1 cut(s) 410
MspA1I CMGCKG 1 cut(s) 1157
MspI CCGG 3 cut(s) 685, 783, 953
MspR9I CCNGG 2 cut(s) 783, 888
MunI CAATTG 1 cut(s) 1360
Mva1269I GAATGC 2 cut(s) 327, 1106
MvaI CCWGG 1 cut(s) 888
MwoI GCNNNNNNNGC 5 cut(s) 324, 1010, 1016, 1025, 1292
NciI CCSGG 1 cut(s) 783
NdeI CATATG 2 cut(s) 136, 211
NdeII GATC 5 cut(s) 139, 573, 785, 1340, 1377
NlaIII CATG 2 cut(s) 55, 415
NlaIV GGNNCC 2 cut(s) 40, 1051
NmuCI GTSAC 2 cut(s) 389, 1394
PaqCI CACCTGC 1 cut(s) 226
PctI GAATGC 2 cut(s) 327, 1106
PfeI GAWTC 1 cut(s) 844
PflFI GACNNNGTC 1 cut(s) 412
PflMI CCANNNNNTGG 1 cut(s) 1365
PinAI ACCGGT 1 cut(s) 952
PkrI GCNGC 7 cut(s) 102, 865, 943, 982, 1012, 1156, 1330
PleI GAGTC 2 cut(s) 84, 688
PpsI GAGTC 2 cut(s) 84, 688
PsiI TTATAA 1 cut(s) 1220
Psp6I CCWGG 1 cut(s) 886
PspEI GGTNACC 1 cut(s) 389
PspGI CCWGG 1 cut(s) 886
PspN4I GGNNCC 2 cut(s) 40, 1051
PspPI GGNCC 1 cut(s) 682
PsyI GACNNNGTC 1 cut(s) 412
PvuII CAGCTG 1 cut(s) 1157
RsaI GTAC 3 cut(s) 542, 633, 762
RsaNI GTAC 3 cut(s) 541, 632, 761
RseI CAYNNNNRTG 1 cut(s) 410
SaqAI TTAA 5 cut(s) 30, 71, 528, 1043, 1077
SatI GCNGC 7 cut(s) 101, 864, 942, 981, 1011, 1155, 1329
Sau3AI GATC 5 cut(s) 139, 573, 785, 1340, 1377
Sau96I GGNCC 1 cut(s) 682
SchI GAGTC 2 cut(s) 85, 688
ScrFI CCNGG 2 cut(s) 783, 888
SduI GDGCHC 2 cut(s) 407, 1181
SfaNI GCATC 6 cut(s) 327, 423, 886, 953, 971, 1006
SinI GGWCC 1 cut(s) 682
SmiMI CAYNNNNRTG 1 cut(s) 410
SmlI CTYRAG 1 cut(s) 332
SmoI CTYRAG 1 cut(s) 332
SsiI CCGC 3 cut(s) 25, 711, 1142
SspI AATATT 1 cut(s) 674
SspMI CTAG 2 cut(s) 87, 153
StyD4I CCNGG 2 cut(s) 781, 886
StyI CCWWGG 2 cut(s) 17, 1081
TaaI ACNGT 6 cut(s) 43, 588, 807, 1092, 1183, 1351
TaiI ACGT 1 cut(s) 978
TaqI TCGA 2 cut(s) 145, 450
TatI WGTACW 1 cut(s) 631
TfiI GAWTC 1 cut(s) 844
Tru1I TTAA 5 cut(s) 30, 71, 528, 1043, 1077
Tru9I TTAA 5 cut(s) 30, 71, 528, 1043, 1077
TscAI CASTG 1 cut(s) 1186
TseFI GTSAC 2 cut(s) 389, 1394
TseI GCWGC 7 cut(s) 100, 863, 941, 980, 1010, 1154, 1328
Tsp45I GTSAC 2 cut(s) 389, 1394
TspDTI ATGAA 2 cut(s) 546, 799
TspGWI ACGGA 1 cut(s) 812
TspRI CASTG 1 cut(s) 1186
Tth111I GACNNNGTC 1 cut(s) 412
Van91I CCANNNNNTGG 1 cut(s) 1365
VneI GTGCAC 2 cut(s) 403, 1177
VpaK11BI GGWCC 1 cut(s) 682
XapI RAATTY 4 cut(s) 108, 118, 1136, 1300
XmiI GTMKAC 1 cut(s) 778
XspI CTAG 2 cut(s) 87, 153
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.