Rroxscaffold_3G00269980

protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0001220
Physical Location & Seq
Forward (+)
62682806 .. 62687039
4234 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00269980.1

Sequence Viewer

Length: 2052 bp
ATGTCGAAGCTGATTCTCCTCCACGCTTCCCACCACCTCACCCCAATCCACACCCGAGCGTTCACCGCCGTCGTCGGAGCCTCTCTCTCCACGCCGCTCCTCCACTCTCACTGCCGTCCTCTCTTCTCTCACTCTCCTCCTCCTCTCTCTCTCCGCCGCCGCCTCTCTGTGCGCGCCTTCGACGAATCGTCGTCCACCACCAAATCAGACGAATTGACCGAAAACGGCGCCGTTTCAGCTCCCGAGGCTAAAATCGATGCCGAGAATGGCAAAGCTACGACGGAGAAGGACTATCCGAGCGGCGAGTTTCATTTTCAGGAGGCGAGTCTCTGGAAGAGCTTCGTCGTCAAGCTTCGGATGCTCTTTGCTTATCCTTGGGAGCGGGTCAAGAAGGGAAGCGTCTTGACCATGACATTGCGCGGCCAGATAACCGATCAGTTAAACAGTCGATTCTCCAGTGGATTATCGCTGCCTCAAATTTGTGAGAATTTTGTGAAAGCTGCGTATGATCCTCGAATTGCTGGTGTGTATCTTCAAATTGAGAGCTTGAACTGTGGTTGGGGGAAAGTGGAAGAAATTCGGAGGCATATATTGGACTTCCAGAAATCAGGTAAGTTTGTTGTGGCGTATGCACCTGCTTGTAGCGAAAAAGAGTATTACCTTGCTTCAGCTTGTCAAGAGATTTATGCCCCTCCAAGTGCTTATTTTTCTTTGTTTGGTCTCAGTGTTCAGGCATCATTTGTAAGAGGTGTTCTTGAGAAAATTGGAGTTGAGCCACAAGTGGAAAGGATTGGTAAATACAAAAGTGCTGGTGACCAACTTGCACGCACGACCATGTCCGAAGAAAATTGTGAGATGCTGACAGCATTGCTCGATAACATATATGGGAACTGGCTGGACATAATTTCTTCTACAAGAGGAAAAAAGAGAGAAGATATTGAGAAGTTTGTTAATGAAGGGGTGTACCAAGTAGAAAAGCTGAAAGATGAGGGCTGGATCACAAACATACAGTATGATGATGAGGTTACTTCAATGTTGAAGGAAAGACTTGGAGTACAAAAAGAGAAAAAGCTTCCTATGGTTGATTACAGGAAATATTCTAAGGTCCGGAAATGGACTCTTGGTTTATCAGGCGGTAAAGACAAGATAGCCATAATAAGAGCCTCAGGCAGTATTAGTCGTGTACGAGGTTCTTTTAGTCTACCTGGATCATCTATTGTTGGAGAACAGTTCATTGAGAAGATCCGTACTATAAGAGAATCAAAAAGATACAAGGCTGCTATTATCCGCATTGACAGCCCTGGAGGTGATGCCCTTGCTTCTGATTTGATGTGGAGGGAAATCAAACTTTTGGCTGCATCAAAACCGGTCATTGCATCAATGTCTGATGTGGCAGCAAGTGGAGGATACTATATGGCAATGGCAGCAGATGCCATTGTTGCAGAAAATCTAACCTTAACTGGTTCCATTGGAGTTGTCACAGGGAAGTTTAACCTGGGAAAACTGTATGAGAAGATTGGCTTCAACAAGGAAATCATATCAAGGGGGAAATTTGCAGAGGTTCTTGCAGCTGAACAACGACCCTTCAAACCAGAGGAAGCAGAACTCTTTGCTAAGTCTGCACAGAATTCGTATAAGCAATTTCGGGACAAGGCAGCTTCTTCCAGATCAATGACTGTAGATAAAATGGAGGAAGTTGCACAAGGGAGAGTTTGGGCTGGTAAAGATGCAGCTTCAAGGGGTTTAGTTGATGCTATTGGTGGACTCTCTCGGGCTGTTGCAATAGCAAAGCTCAAGGCAAAGATACCACAAGACACAGAGGTTACACTAGTGGAACTTGCAAGACCCTCCCCTTCACTGCCAGAACTTTTAAGTGGCATAGGGAGTACTCTGGTTGGAGTTGAGCAAACGATGAAGGCACTGCTGCAAGACTTAACATTTTCTGATGGAGTTCAAGCCCGGATGGACGGAATCTTGTTTCAGAGACTGGAGGGTGCTTCTAATGCCAACCCCATCTTCAATTTGATAAGAGATTACCTTGGTTCTCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_S49

Protein Analysis

683

Amino Acids

75.32

Weight (kDa)

9.08

Isoelectric Point (pI)

38.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S49 PF01343 200 - 352 9.1e-24 Peptidase family S49
Peptidase_S49 PF01343 451 - 601 3.1e-42 Peptidase family S49
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 643
Acc36I ACCTGC 1 cut(s) 643
AccB1I GGYRCC 1 cut(s) 227
AccBSI CCGCTC 3 cut(s) 97, 300, 382
AccI GTMKAC 1 cut(s) 1201
AccII CGCG 2 cut(s) 174, 420
AccIII TCCGGA 1 cut(s) 1107
AclWI GGATC 4 cut(s) 503, 1004, 1216, 1237
AcoI YGGCCR 1 cut(s) 421
AcsI RAATTY 5 cut(s) 477, 487, 576, 1550, 1627
AcuI CTGAAG 1 cut(s) 651
AcyI GRCGYC 1 cut(s) 228
AfaI GTAC 5 cut(s) 965, 1056, 1185, 1249, 1888
AgeI ACCGGT 1 cut(s) 1366
AgsI TTSAA 9 cut(s) 536, 550, 1032, 1039, 1525, 1588, 1737, 1955, 2020
AhdI GACNNNNNGTC 1 cut(s) 187
AhlI ACTAGT 1 cut(s) 1828
AjnI CCWGG 3 cut(s) 1204, 1300, 1494
AjuI GAANNNNNNNTTGG 2 cut(s) 2000, 2032
Alw26I GTCTC 3 cut(s) 332, 725, 1978
AlwI GGATC 4 cut(s) 503, 1004, 1216, 1237
AlwNI CAGNNNCTG 1 cut(s) 1987
Ama87I CYCGRG 3 cut(s) 54, 242, 1770
Aor13HI TCCGGA 1 cut(s) 1107
AoxI GGCC 1 cut(s) 421
ApoI RAATTY 5 cut(s) 477, 487, 576, 1550, 1627
AsiGI ACCGGT 1 cut(s) 1366
Asp700I GAANNNNTTC 2 cut(s) 338, 576
AspLEI GCGC 4 cut(s) 174, 176, 230, 420
AspS9I GGNCC 1 cut(s) 1105
AsuC2I CCSGG 1 cut(s) 1960
AsuHPI GGTGA 4 cut(s) 31, 55, 824, 1319
AvaI CYCGRG 3 cut(s) 54, 242, 1770
AvaII GGWCC 1 cut(s) 1105
AxyI CCTNAGG 1 cut(s) 1165
BanI GGYRCC 1 cut(s) 227
BccI CCATC 3 cut(s) 1940, 1957, 2021
BceAI ACGGC 4 cut(s) 53, 99, 215, 241
BcgI CGANNNNNNTGC 2 cut(s) 1611, 1645
BciT130I CCWGG 3 cut(s) 1206, 1302, 1496
BciVI GTATCC 1 cut(s) 1400
BcnI CCSGG 1 cut(s) 1960
BcoDI GTCTC 3 cut(s) 332, 725, 1978
BcuI ACTAGT 1 cut(s) 1828
BfaI CTAG 1 cut(s) 1829
BfmI CTRYAG 1 cut(s) 1677
BfoI RGCGCY 1 cut(s) 231
BfuAI ACCTGC 1 cut(s) 643
BfuI GTATCC 1 cut(s) 1400
BmcAI AGTACT 1 cut(s) 1888
Bme1390I CCNGG 4 cut(s) 1206, 1302, 1496, 1960
Bme18I GGWCC 1 cut(s) 1105
BmeRI GACNNNNNGTC 1 cut(s) 187
BmeT110I CYCGRG 3 cut(s) 54, 242, 1770
BmgT120I GGNCC 1 cut(s) 1105
BmiI GGNNCC 3 cut(s) 79, 229, 1465
BmrFI CCNGG 4 cut(s) 1206, 1302, 1496, 1960
BplI GAGNNNNNCTC 2 cut(s) 69, 101
BpmI CTGGAG 3 cut(s) 439, 1323, 2009
BpuEI CTTGAG 2 cut(s) 776, 1778
BpuMI CCSGG 1 cut(s) 1960
Bsa29I ATCGAT 1 cut(s) 255
BsaHI GRCGYC 1 cut(s) 228
BsaI GGTCTC 1 cut(s) 725
BsaJI CCNNGG 5 cut(s) 243, 374, 1300, 1495, 2038
BsaWI WCCGGW 2 cut(s) 1107, 1366
Bse118I RCCGGY 1 cut(s) 1366
Bse1I ACTGG 4 cut(s) 456, 896, 1465, 1992
Bse21I CCTNAGG 1 cut(s) 1165
Bse3DI GCAATG 4 cut(s) 413, 866, 1371, 1425
BseAI TCCGGA 1 cut(s) 1107
BseBI CCWGG 3 cut(s) 1206, 1302, 1496
BseCI ATCGAT 1 cut(s) 255
BseDI CCNNGG 5 cut(s) 243, 374, 1300, 1495, 2038
BseGI GGATG 2 cut(s) 363, 1968
BseMI GCAATG 4 cut(s) 413, 866, 1371, 1425
BseMII CTCAG 2 cut(s) 736, 1179
BseNI ACTGG 4 cut(s) 456, 896, 1465, 1992
BsePI GCGCGC 1 cut(s) 172
BseRI GAGGAG 5 cut(s) 8, 89, 126, 129, 132
BsgI GTGCAG 1 cut(s) 1605
Bsh1236I CGCG 2 cut(s) 174, 420
BshFI GGCC 1 cut(s) 423
BshNI GGYRCC 1 cut(s) 227
BshTI ACCGGT 1 cut(s) 1366
BshVI ATCGAT 1 cut(s) 255
BsiHKCI CYCGRG 3 cut(s) 54, 242, 1770
BsiSI CCGG 3 cut(s) 1108, 1367, 1960
BslFI GGGAC 1 cut(s) 1661
BsmAI GTCTC 3 cut(s) 332, 725, 1978
BsmFI GGGAC 1 cut(s) 1661
BsnI GGCC 1 cut(s) 423
Bso31I GGTCTC 1 cut(s) 725
BsoBI CYCGRG 3 cut(s) 54, 242, 1770
Bsp13I TCCGGA 1 cut(s) 1107
Bsp143I GATC 6 cut(s) 433, 508, 996, 1208, 1242, 1667
BspANI GGCC 1 cut(s) 423
BspCNI CTCAG 2 cut(s) 735, 1178
BspDI ATCGAT 1 cut(s) 255
BspEI TCCGGA 1 cut(s) 1107
BspFNI CGCG 2 cut(s) 174, 420
BspLI GGNNCC 3 cut(s) 79, 229, 1465
BspMI ACCTGC 1 cut(s) 643
BspPI GGATC 4 cut(s) 503, 1004, 1216, 1237
BspQI GCTCTTC 1 cut(s) 329
BspT107I GGYRCC 1 cut(s) 227
BspTNI GGTCTC 1 cut(s) 725
BsrBI CCGCTC 3 cut(s) 97, 300, 382
BsrDI GCAATG 4 cut(s) 413, 866, 1371, 1425
BsrFI RCCGGY 1 cut(s) 1366
BsrI ACTGG 4 cut(s) 456, 896, 1465, 1992
BssAI RCCGGY 1 cut(s) 1366
BssECI CCNNGG 5 cut(s) 243, 374, 1300, 1495, 2038
BssHII GCGCGC 1 cut(s) 172
BssMI GATC 6 cut(s) 433, 508, 996, 1208, 1242, 1667
BssNI GRCGYC 1 cut(s) 228
BssT1I CCWWGG 2 cut(s) 374, 2038
Bst2UI CCWGG 3 cut(s) 1206, 1302, 1496
Bst4CI ACNGT 6 cut(s) 446, 554, 1011, 1230, 1506, 1678
Bst6I CTCTTC 2 cut(s) 128, 329
BstACI GRCGYC 1 cut(s) 228
BstAPI GCANNNNNTGC 1 cut(s) 1430
BstC8I GCNNGC 2 cut(s) 174, 826
BstDEI CTNAG 4 cut(s) 722, 1101, 1165, 1614
BstEII GGTNACC 1 cut(s) 812
BstF5I GGATG 2 cut(s) 363, 1968
BstFNI CGCG 2 cut(s) 174, 420
BstH2I RGCGCY 1 cut(s) 231
BstHHI GCGC 4 cut(s) 174, 176, 230, 420
BstKTI GATC 6 cut(s) 436, 511, 999, 1211, 1245, 1670
BstMAI GTCTC 3 cut(s) 332, 725, 1978
BstMBI GATC 6 cut(s) 433, 508, 996, 1208, 1242, 1667
BstNI CCWGG 3 cut(s) 1206, 1302, 1496
BstPI GGTNACC 1 cut(s) 812
BstSCI CCNGG 4 cut(s) 1204, 1300, 1494, 1958
BstSFI CTRYAG 1 cut(s) 1677
BstUI CGCG 2 cut(s) 174, 420
BstX2I RGATCY 1 cut(s) 1242
BstYI RGATCY 1 cut(s) 1242
Bsu15I ATCGAT 1 cut(s) 255
Bsu36I CCTNAGG 1 cut(s) 1165
BsuI GTATCC 1 cut(s) 1400
BsuRI GGCC 1 cut(s) 423
BsuTUI ATCGAT 1 cut(s) 255
BtsCI GGATG 2 cut(s) 363, 1968
BtsI GCAGTG 3 cut(s) 109, 1856, 1919
BtsIMutI CAGTG 5 cut(s) 109, 463, 730, 1856, 1919
BveI ACCTGC 1 cut(s) 643
Cac8I GCNNGC 2 cut(s) 174, 826
CaiI CAGNNNCTG 1 cut(s) 1987
CfoI GCGC 4 cut(s) 174, 176, 230, 420
Cfr10I RCCGGY 1 cut(s) 1366
Cfr13I GGNCC 1 cut(s) 1105
ClaI ATCGAT 1 cut(s) 255
CseI GACGC 1 cut(s) 388
Csp6I GTAC 5 cut(s) 964, 1055, 1184, 1248, 1887
CspAI ACCGGT 1 cut(s) 1366
CviAII CATG 2 cut(s) 409, 835
CviQI GTAC 5 cut(s) 964, 1055, 1184, 1248, 1887
DdeI CTNAG 4 cut(s) 722, 1101, 1165, 1614
DinI GGCGCC 1 cut(s) 229
DpnI GATC 6 cut(s) 435, 510, 998, 1210, 1244, 1669
DpnII GATC 6 cut(s) 433, 508, 996, 1208, 1242, 1667
DriI GACNNNNNGTC 1 cut(s) 187
EaeI YGGCCR 1 cut(s) 421
Eam1104I CTCTTC 2 cut(s) 128, 329
Eam1105I GACNNNNNGTC 1 cut(s) 187
EarI CTCTTC 2 cut(s) 128, 329
EciI GGCGGA 1 cut(s) 143
Eco130I CCWWGG 2 cut(s) 374, 2038
Eco31I GGTCTC 1 cut(s) 725
Eco47I GGWCC 1 cut(s) 1105
Eco57I CTGAAG 1 cut(s) 651
Eco81I CCTNAGG 1 cut(s) 1165
Eco88I CYCGRG 3 cut(s) 54, 242, 1770
Eco91I GGTNACC 1 cut(s) 812
EcoO65I GGTNACC 1 cut(s) 812
EcoRI GAATTC 1 cut(s) 1627
EcoRII CCWGG 3 cut(s) 1204, 1300, 1494
EcoT14I CCWWGG 2 cut(s) 374, 2038
EgeI GGCGCC 1 cut(s) 229
EheI GGCGCC 1 cut(s) 229
ErhI CCWWGG 2 cut(s) 374, 2038
FaeI CATG 2 cut(s) 412, 838
FalI AAGNNNNNCTT 2 cut(s) 1505, 1537
FaqI GGGAC 1 cut(s) 1661
FatI CATG 2 cut(s) 408, 834
FauI CCCGC 1 cut(s) 375
FblI GTMKAC 1 cut(s) 1201
FokI GGATG 2 cut(s) 370, 1975
FspBI CTAG 1 cut(s) 1829
GlaI GCGC 4 cut(s) 173, 175, 229, 419
GsuI CTGGAG 3 cut(s) 439, 1323, 2009
HaeII RGCGCY 1 cut(s) 231
HaeIII GGCC 1 cut(s) 423
HapII CCGG 3 cut(s) 1108, 1367, 1960
HgaI GACGC 1 cut(s) 388
HhaI GCGC 4 cut(s) 174, 176, 230, 420
Hin1I GRCGYC 1 cut(s) 228
Hin1II CATG 2 cut(s) 412, 838
Hin6I GCGC 4 cut(s) 172, 174, 228, 418
HinP1I GCGC 4 cut(s) 172, 174, 228, 418
HindIII AAGCTT 2 cut(s) 350, 1070
HinfI GANTC 8 cut(s) 13, 185, 325, 450, 1117, 1259, 1764, 1971
HpaII CCGG 3 cut(s) 1108, 1367, 1960
HphI GGTGA 4 cut(s) 31, 55, 824, 1319
Hpy166II GTNNAC 6 cut(s) 63, 195, 964, 1184, 1202, 1763
Hpy8I GTNNAC 6 cut(s) 63, 195, 964, 1184, 1202, 1763
Hpy99I CGWCG 6 cut(s) 74, 77, 185, 193, 283, 347
HpyAV CCTTC 8 cut(s) 187, 280, 385, 950, 1033, 1594, 1863, 1909
HpyCH4III ACNGT 6 cut(s) 446, 554, 1011, 1230, 1506, 1678
HpyF3I CTNAG 4 cut(s) 722, 1101, 1165, 1614
Hsp92I GRCGYC 1 cut(s) 228
Hsp92II CATG 2 cut(s) 412, 838
HspAI GCGC 4 cut(s) 172, 174, 228, 418
KasI GGCGCC 1 cut(s) 227
Kpn2I TCCGGA 1 cut(s) 1107
Kzo9I GATC 6 cut(s) 433, 508, 996, 1208, 1242, 1667
LguI GCTCTTC 1 cut(s) 329
LmnI GCTCC 4 cut(s) 77, 102, 244, 379
MaeI CTAG 1 cut(s) 1829
MaeIII GTNAC 4 cut(s) 812, 1024, 1477, 1822
MalI GATC 6 cut(s) 435, 510, 998, 1210, 1244, 1669
MbiI CCGCTC 3 cut(s) 97, 300, 382
MboI GATC 6 cut(s) 433, 508, 996, 1208, 1242, 1667
MflI RGATCY 1 cut(s) 1242
Mly113I GGCGCC 1 cut(s) 228
MlyI GAGTC 3 cut(s) 334, 1111, 1758
MmeI TCCRAC 3 cut(s) 55, 1201, 1876
MroI TCCGGA 1 cut(s) 1107
MroXI GAANNNNTTC 2 cut(s) 338, 576
MseI TTAA 6 cut(s) 440, 951, 1457, 1491, 1871, 1934
MslI CAYNNNNRTG 1 cut(s) 833
MspA1I CMGCKG 1 cut(s) 1571
MspI CCGG 3 cut(s) 1108, 1367, 1960
MspR9I CCNGG 4 cut(s) 1206, 1302, 1496, 1960
MvaI CCWGG 3 cut(s) 1206, 1302, 1496
MvnI CGCG 2 cut(s) 174, 420
NarI GGCGCC 1 cut(s) 228
NciI CCSGG 1 cut(s) 1960
NdeII GATC 6 cut(s) 433, 508, 996, 1208, 1242, 1667
NlaIII CATG 2 cut(s) 412, 838
NlaIV GGNNCC 3 cut(s) 79, 229, 1465
NmeAIII GCCGAG 1 cut(s) 286
NmuCI GTSAC 2 cut(s) 812, 1477
PaqCI CACCTGC 1 cut(s) 643
PauI GCGCGC 1 cut(s) 172
PciSI GCTCTTC 1 cut(s) 329
PcsI WCGNNNNNNNCGW 1 cut(s) 216
PdmI GAANNNNTTC 2 cut(s) 338, 576
PfeI GAWTC 5 cut(s) 13, 185, 450, 1259, 1971
PflFI GACNNNGTC 1 cut(s) 835
PinAI ACCGGT 1 cut(s) 1366
PleI GAGTC 3 cut(s) 333, 1111, 1758
PluTI GGCGCC 1 cut(s) 231
PpsI GAGTC 3 cut(s) 333, 1111, 1758
Psp6I CCWGG 3 cut(s) 1204, 1300, 1494
PspEI GGTNACC 1 cut(s) 812
PspGI CCWGG 3 cut(s) 1204, 1300, 1494
PspN4I GGNNCC 3 cut(s) 79, 229, 1465
PspPI GGNCC 1 cut(s) 1105
PsrI GAACNNNNNNTAC 2 cut(s) 735, 767
PstNI CAGNNNCTG 1 cut(s) 1987
PsuI RGATCY 1 cut(s) 1242
PsyI GACNNNGTC 1 cut(s) 835
PteI GCGCGC 1 cut(s) 172
PvuII CAGCTG 1 cut(s) 1571
RsaI GTAC 5 cut(s) 965, 1056, 1185, 1249, 1888
RsaNI GTAC 5 cut(s) 964, 1055, 1184, 1248, 1887
RseI CAYNNNNRTG 1 cut(s) 833
SapI GCTCTTC 1 cut(s) 329
SaqAI TTAA 6 cut(s) 440, 951, 1457, 1491, 1871, 1934
Sau3AI GATC 6 cut(s) 433, 508, 996, 1208, 1242, 1667
Sau96I GGNCC 1 cut(s) 1105
ScaI AGTACT 1 cut(s) 1888
SchI GAGTC 3 cut(s) 334, 1111, 1758
ScrFI CCNGG 4 cut(s) 1206, 1302, 1496, 1960
SfcI CTRYAG 1 cut(s) 1677
SfoI GGCGCC 1 cut(s) 229
SinI GGWCC 1 cut(s) 1105
SmiMI CAYNNNNRTG 1 cut(s) 833
SmlI CTYRAG 2 cut(s) 755, 1793
SmoI CTYRAG 2 cut(s) 755, 1793
SpeI ACTAGT 1 cut(s) 1828
SspDI GGCGCC 1 cut(s) 227
SspI AATATT 1 cut(s) 1097
SspMI CTAG 1 cut(s) 1829
StyD4I CCNGG 4 cut(s) 1204, 1300, 1494, 1958
StyI CCWWGG 2 cut(s) 374, 2038
TaaI ACNGT 6 cut(s) 446, 554, 1011, 1230, 1506, 1678
TaqI TCGA 6 cut(s) 5, 180, 255, 448, 514, 873
TaqII GACCGA 1 cut(s) 233
TatI WGTACW 2 cut(s) 1054, 1886
TauI GCSGC 5 cut(s) 97, 159, 162, 303, 423
TfiI GAWTC 5 cut(s) 13, 185, 450, 1259, 1971
Tru1I TTAA 6 cut(s) 440, 951, 1457, 1491, 1871, 1934
Tru9I TTAA 6 cut(s) 440, 951, 1457, 1491, 1871, 1934
TscAI CASTG 5 cut(s) 116, 463, 730, 1863, 1926
TseFI GTSAC 2 cut(s) 812, 1477
Tsp45I GTSAC 2 cut(s) 812, 1477
TspDTI ATGAA 4 cut(s) 299, 969, 1222, 1928
TspGWI ACGGA 3 cut(s) 296, 1235, 1983
TspRI CASTG 5 cut(s) 116, 463, 730, 1863, 1926
Tth111I GACNNNGTC 1 cut(s) 835
VpaK11BI GGWCC 1 cut(s) 1105
XapI RAATTY 5 cut(s) 477, 487, 576, 1550, 1627
XmiI GTMKAC 1 cut(s) 1201
XmnI GAANNNNTTC 2 cut(s) 338, 576
XspI CTAG 1 cut(s) 1829
ZrmI AGTACT 1 cut(s) 1888
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.