Rorug06G0461700

protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0001220
Physical Location & Seq
Forward (+)
58765310 .. 58770608
5299 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0461700.1

Sequence Viewer

Length: 1254 bp
ATGGGTCGCTTAGTCCTCTCTCACTTCCTCACCATCTCCAACTCTTCTCCTCGCCTCGCTTCTTCCTCACTGCGCGCGGGTGGATTTCAAATCAGGCACTTGAAAGGAGCTAGTTCAAGTCCTCAGAATTGGCTTCCGAGTCCACGAAGCTCCGCCGGATTCCGATGCTGTTGCAGCGTTTCGCTCGCGCAACCGGCTGTTTCCGAGAGTTCTCCGACCACTGTAAAGAAGAGAATAGTGTCTGGAGTCCAGCCGACAGGTTCTATCCACCTGGGGAATTATCTCGGTGCCATACGAAACTGGATTACATTACAGAATACATACGATACTCTCTTTTTCATTGTGGACCTCCATGCGATTACATTACCGTATGATGCACAACAATTATCCAGGGCAACGAGGGATACAGCAGCTCTTTATTTGGCATGTGGAGTTGACACCTCGAAGGCATCTGTCTTCGTGCAGTCTCATGTTCGTGCCCATGTAGAACTGATGTGGCTTTTAAGTTCTGTAGCACCTGTTGGATGGCTTAACAGAATGATTCAGTTTAAAGAGAAATCACGCAAGGCGGGGGATGAAAATGTTGGTGTTGCTCTCTTGACTTACCCTGTTTTGATGGCGTCTGATATTCTTTTATATCAGTCTGACTTTGTCCCAGTCGGTGAAGATCAAAAGCAGCATTTGGAGTTGACACGTGAGCTGGCTGAGCGTGTTAATAATTTATTTGGAGGACGGAAATGGAAAAAATTAGGAGGGAGAGGAGGTGCACTTTTCAAGGTCCCTGAGCCTCTTATACCACCAGTTGGAGCTCGAGTGATGTCCCTTACTGATGGTTGTTCCAAGATGTCCAAATCGGCGCCTTCTGATCAGTCGCGGATAAATCTTCTTGACCCAAAAGATGTAATAGCTAACAAGATAAAGAGGTGCAAAACAGACTCATTTCCAGGCATGGAATTTGATAATCCAGAAAGGCCTGAGTGCAATAATCTTCTCTCAATATATCAGCTCATATCGGGAAAGACAAAAGAGGAAGTTGCACAAGAATGCCAAGATATGAACTGGGGCACATTTAAGTCACTCCTGGCTGACGCGTTGATTGATCATCTGTATCCTATCCAGGTTCGCTACGAGGAAATCATTTCTGATGCAGCTTATTTGGATGGAATTTTGGCAGAAGGTGCCACGAAAGCTTCAGATATAGCTGATACTACTCTCAATAATGTTTATCAGGCTATGGGATTCTTGCGGAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_S49

Protein Analysis

417

Amino Acids

45.89

Weight (kDa)

8.8

Isoelectric Point (pI)

46.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
tRNA-synt_1b PF00579 77 - 369 2.2e-80 tRNA synthetases class I (W and Y)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 287, 856, 1178
AccB7I CCANNNNNTGG 1 cut(s) 803
AccII CGCG 5 cut(s) 75, 77, 188, 874, 1091
AciI CCGC 5 cut(s) 77, 153, 569, 874, 1246
AcsI RAATTY 2 cut(s) 953, 1164
AcuI CTGAAG 1 cut(s) 1176
AcvI CACGTG 1 cut(s) 695
AcyI GRCGYC 2 cut(s) 620, 857
AfiI CCNNNNNNNGG 1 cut(s) 803
AflIII ACRYGT 2 cut(s) 692, 1089
AgsI TTSAA 4 cut(s) 89, 103, 117, 775
AjnI CCWGG 5 cut(s) 270, 389, 943, 1080, 1116
Alw21I GWGCWC 2 cut(s) 769, 811
Alw26I GTCTC 1 cut(s) 471
Alw44I GTGCAC 1 cut(s) 765
Ama87I CYCGRG 1 cut(s) 810
AoxI GGCC 1 cut(s) 971
ApaLI GTGCAC 1 cut(s) 765
ApeKI GCWGC 4 cut(s) 174, 410, 676, 1148
ApoI RAATTY 2 cut(s) 953, 1164
AspLEI GCGC 4 cut(s) 75, 77, 190, 859
AspS9I GGNCC 2 cut(s) 346, 778
AsuHPI GGTGA 2 cut(s) 22, 674
AvaI CYCGRG 1 cut(s) 810
AvaII GGWCC 2 cut(s) 346, 778
BaeGI GKGCMC 3 cut(s) 481, 769, 1067
BanI GGYRCC 3 cut(s) 287, 856, 1178
BanII GRGCYC 1 cut(s) 811
BbrPI CACGTG 1 cut(s) 695
BbsI GAAGAC 1 cut(s) 448
Bbv12I GWGCWC 2 cut(s) 769, 811
BbvI GCAGC 4 cut(s) 186, 422, 688, 1160
BccI CCATC 5 cut(s) 41, 519, 610, 824, 1154
BcgI CGANNNNNNTGC 2 cut(s) 153, 187
BciT130I CCWGG 5 cut(s) 272, 391, 945, 1082, 1118
BciVI GTATCC 2 cut(s) 397, 1119
BclI TGATCA 2 cut(s) 865, 1099
BcoDI GTCTC 1 cut(s) 471
BfaI CTAG 1 cut(s) 111
BfmI CTRYAG 1 cut(s) 510
BfoI RGCGCY 1 cut(s) 860
BfuI GTATCC 2 cut(s) 397, 1119
BisI GCNGC 4 cut(s) 175, 411, 677, 1149
BlpI GCTNAGC 1 cut(s) 705
BlsI GCNGC 4 cut(s) 176, 412, 678, 1150
Bme1390I CCNGG 5 cut(s) 272, 391, 945, 1082, 1118
Bme18I GGWCC 2 cut(s) 346, 778
BmeT110I CYCGRG 1 cut(s) 810
BmgT120I GGNCC 2 cut(s) 346, 778
BmiI GGNNCC 4 cut(s) 289, 780, 858, 1180
BmrFI CCNGG 5 cut(s) 272, 391, 945, 1082, 1118
BmrI ACTGGG 2 cut(s) 650, 1069
BmsI GCATC 4 cut(s) 155, 364, 458, 1135
BmuI ACTGGG 2 cut(s) 650, 1069
BpiI GAAGAC 1 cut(s) 448
BpmI CTGGAG 1 cut(s) 264
Bpu10I CCTNAGC 1 cut(s) 783
Bpu1102I GCTNAGC 1 cut(s) 705
BsaAI YACGTR 1 cut(s) 695
BsaHI GRCGYC 2 cut(s) 620, 857
BsaJI CCNNGG 2 cut(s) 271, 390
BsaXI ACNNNNNCTCC 4 cut(s) 748, 778, 798, 828
Bsc4I CCNNNNNNNGG 1 cut(s) 803
Bse118I RCCGGY 1 cut(s) 193
Bse1I ACTGG 4 cut(s) 305, 656, 800, 1064
BseBI CCWGG 5 cut(s) 272, 391, 945, 1082, 1118
BseDI CCNNGG 2 cut(s) 271, 390
BseGI GGATG 3 cut(s) 530, 580, 1165
BseLI CCNNNNNNNGG 1 cut(s) 803
BseMII CTCAG 4 cut(s) 137, 696, 774, 966
BseNI ACTGG 4 cut(s) 305, 656, 800, 1064
BsePI GCGCGC 1 cut(s) 73
BseRI GAGGAG 2 cut(s) 39, 774
BseSI GKGCMC 3 cut(s) 481, 769, 1067
BseXI GCAGC 4 cut(s) 186, 422, 688, 1160
BsgI GTGCAG 1 cut(s) 482
Bsh1236I CGCG 5 cut(s) 75, 77, 188, 874, 1091
BshFI GGCC 1 cut(s) 973
BshNI GGYRCC 3 cut(s) 287, 856, 1178
BsiHKAI GWGCWC 2 cut(s) 769, 811
BsiHKCI CYCGRG 1 cut(s) 810
BsiSI CCGG 2 cut(s) 156, 194
BslFI GGGAC 3 cut(s) 638, 764, 805
BslI CCNNNNNNNGG 1 cut(s) 803
BsmAI GTCTC 1 cut(s) 471
BsmFI GGGAC 3 cut(s) 638, 764, 805
BsmI GAATGC 1 cut(s) 1049
BsnI GGCC 1 cut(s) 973
BsoBI CYCGRG 1 cut(s) 810
Bsp1286I GDGCHC 4 cut(s) 481, 769, 811, 1067
Bsp143I GATC 3 cut(s) 667, 865, 1099
Bsp1720I GCTNAGC 1 cut(s) 705
BspACI CCGC 5 cut(s) 77, 153, 569, 874, 1246
BspANI GGCC 1 cut(s) 973
BspCNI CTCAG 4 cut(s) 136, 697, 775, 967
BspFNI CGCG 5 cut(s) 75, 77, 188, 874, 1091
BspLI GGNNCC 4 cut(s) 289, 780, 858, 1180
BspT107I GGYRCC 3 cut(s) 287, 856, 1178
BsrFI RCCGGY 1 cut(s) 193
BsrI ACTGG 4 cut(s) 305, 656, 800, 1064
BssAI RCCGGY 1 cut(s) 193
BssECI CCNNGG 2 cut(s) 271, 390
BssHII GCGCGC 1 cut(s) 73
BssMI GATC 3 cut(s) 667, 865, 1099
BssNI GRCGYC 2 cut(s) 620, 857
Bst2UI CCWGG 5 cut(s) 272, 391, 945, 1082, 1118
Bst4CI ACNGT 2 cut(s) 223, 369
Bst6I CTCTTC 2 cut(s) 49, 224
BstACI GRCGYC 2 cut(s) 620, 857
BstAPI GCANNNNNTGC 1 cut(s) 1178
BstBAI YACGTR 1 cut(s) 695
BstC8I GCNNGC 3 cut(s) 75, 186, 702
BstDEI CTNAG 5 cut(s) 10, 123, 705, 783, 975
BstF5I GGATG 3 cut(s) 530, 580, 1165
BstFNI CGCG 5 cut(s) 75, 77, 188, 874, 1091
BstH2I RGCGCY 1 cut(s) 860
BstHHI GCGC 4 cut(s) 75, 77, 190, 859
BstKTI GATC 3 cut(s) 670, 868, 1102
BstMAI GTCTC 1 cut(s) 471
BstMBI GATC 3 cut(s) 667, 865, 1099
BstMWI GCNNNNNNNGC 5 cut(s) 174, 194, 706, 1178, 1187
BstNI CCWGG 5 cut(s) 272, 391, 945, 1082, 1118
BstNSI RCATGY 1 cut(s) 429
BstSCI CCNGG 5 cut(s) 270, 389, 943, 1080, 1116
BstSFI CTRYAG 1 cut(s) 510
BstSLI GKGCMC 3 cut(s) 481, 769, 1067
BstUI CGCG 5 cut(s) 75, 77, 188, 874, 1091
BstV1I GCAGC 4 cut(s) 186, 422, 688, 1160
BstV2I GAAGAC 1 cut(s) 448
BsuI GTATCC 2 cut(s) 397, 1119
BsuRI GGCC 1 cut(s) 973
BtsCI GGATG 3 cut(s) 530, 580, 1165
BtsI GCAGTG 1 cut(s) 68
BtsIMutI CAGTG 2 cut(s) 68, 219
Cac8I GCNNGC 3 cut(s) 75, 186, 702
CfoI GCGC 4 cut(s) 75, 77, 190, 859
Cfr10I RCCGGY 1 cut(s) 193
Cfr13I GGNCC 2 cut(s) 346, 778
CseI GACGC 2 cut(s) 609, 1097
CviAII CATG 5 cut(s) 353, 426, 470, 482, 949
DdeI CTNAG 5 cut(s) 10, 123, 705, 783, 975
DinI GGCGCC 1 cut(s) 858
DpnI GATC 3 cut(s) 669, 867, 1101
DpnII GATC 3 cut(s) 667, 865, 1099
DraI TTTAAA 1 cut(s) 550
Eam1104I CTCTTC 2 cut(s) 49, 224
EarI CTCTTC 2 cut(s) 49, 224
EciI GGCGGA 1 cut(s) 142
Ecl136II GAGCTC 1 cut(s) 809
Eco147I AGGCCT 1 cut(s) 973
Eco24I GRGCYC 1 cut(s) 811
Eco47I GGWCC 2 cut(s) 346, 778
Eco53kI GAGCTC 1 cut(s) 809
Eco57I CTGAAG 1 cut(s) 1176
Eco72I CACGTG 1 cut(s) 695
Eco88I CYCGRG 1 cut(s) 810
EcoICRI GAGCTC 1 cut(s) 809
EcoO109I RGGNCCY 1 cut(s) 778
EcoRII CCWGG 5 cut(s) 270, 389, 943, 1080, 1116
EcoT38I GRGCYC 1 cut(s) 811
EgeI GGCGCC 1 cut(s) 858
EheI GGCGCC 1 cut(s) 858
FaeI CATG 5 cut(s) 356, 429, 473, 485, 952
FaqI GGGAC 3 cut(s) 638, 764, 805
FatI CATG 5 cut(s) 352, 425, 469, 481, 948
FauI CCCGC 2 cut(s) 70, 562
FbaI TGATCA 2 cut(s) 865, 1099
Fnu4HI GCNGC 4 cut(s) 175, 411, 677, 1149
FokI GGATG 3 cut(s) 537, 587, 1172
FriOI GRGCYC 1 cut(s) 811
Fsp4HI GCNGC 4 cut(s) 175, 411, 677, 1149
FspBI CTAG 1 cut(s) 111
GlaI GCGC 4 cut(s) 74, 76, 189, 858
GluI GCNGC 4 cut(s) 175, 411, 677, 1149
GsuI CTGGAG 1 cut(s) 264
HaeII RGCGCY 1 cut(s) 860
HaeIII GGCC 1 cut(s) 973
HapII CCGG 2 cut(s) 156, 194
HgaI GACGC 2 cut(s) 609, 1097
HhaI GCGC 4 cut(s) 75, 77, 190, 859
Hin1I GRCGYC 2 cut(s) 620, 857
Hin1II CATG 5 cut(s) 356, 429, 473, 485, 952
Hin6I GCGC 4 cut(s) 73, 75, 188, 857
HinP1I GCGC 4 cut(s) 73, 75, 188, 857
HincII GTYRAC 2 cut(s) 436, 690
HindII GTYRAC 2 cut(s) 436, 690
HindIII AAGCTT 1 cut(s) 1188
HinfI GANTC 6 cut(s) 139, 159, 246, 541, 935, 1239
HpaII CCGG 2 cut(s) 156, 194
HphI GGTGA 2 cut(s) 22, 674
Hpy166II GTNNAC 5 cut(s) 143, 346, 436, 690, 767
Hpy188III TCNNGA 5 cut(s) 243, 598, 887, 965, 1014
Hpy8I GTNNAC 5 cut(s) 143, 346, 436, 690, 767
HpyAV CCTTC 3 cut(s) 439, 870, 1169
HpyCH4III ACNGT 2 cut(s) 223, 369
HpyCH4IV ACGT 1 cut(s) 694
HpyCH4V TGCA 8 cut(s) 174, 377, 463, 767, 927, 981, 1037, 1148
HpyF10VI GCNNNNNNNGC 5 cut(s) 174, 194, 706, 1178, 1187
HpyF3I CTNAG 5 cut(s) 10, 123, 705, 783, 975
HpySE526I ACGT 1 cut(s) 694
Hsp92I GRCGYC 2 cut(s) 620, 857
Hsp92II CATG 5 cut(s) 356, 429, 473, 485, 952
HspAI GCGC 4 cut(s) 73, 75, 188, 857
KasI GGCGCC 1 cut(s) 856
Ksp22I TGATCA 2 cut(s) 865, 1099
Kzo9I GATC 3 cut(s) 667, 865, 1099
LmnI GCTCC 3 cut(s) 107, 155, 806
Lsp1109I GCAGC 4 cut(s) 186, 422, 688, 1160
LweI GCATC 4 cut(s) 155, 364, 458, 1135
MaeI CTAG 1 cut(s) 111
MaeII ACGT 1 cut(s) 694
MaeIII GTNAC 1 cut(s) 1074
MalI GATC 3 cut(s) 669, 867, 1101
MboI GATC 3 cut(s) 667, 865, 1099
MboII GAAGA 7 cut(s) 36, 54, 241, 448, 677, 875, 980
MhlI GDGCHC 4 cut(s) 481, 769, 811, 1067
MluCI AATT 7 cut(s) 127, 277, 383, 718, 746, 953, 1164
MluI ACGCGT 1 cut(s) 1089
Mly113I GGCGCC 1 cut(s) 857
MlyI GAGTC 3 cut(s) 148, 255, 929
MmeI TCCRAC 4 cut(s) 63, 239, 502, 784
MseI TTAA 5 cut(s) 503, 531, 549, 714, 1071
MslI CAYNNNNRTG 2 cut(s) 474, 1042
MspI CCGG 2 cut(s) 156, 194
MspR9I CCNGG 5 cut(s) 272, 391, 945, 1082, 1118
Mva1269I GAATGC 1 cut(s) 1049
MvaI CCWGG 5 cut(s) 272, 391, 945, 1082, 1118
MvnI CGCG 5 cut(s) 75, 77, 188, 874, 1091
MwoI GCNNNNNNNGC 5 cut(s) 174, 194, 706, 1178, 1187
NarI GGCGCC 1 cut(s) 857
NdeII GATC 3 cut(s) 667, 865, 1099
NlaIII CATG 5 cut(s) 356, 429, 473, 485, 952
NlaIV GGNNCC 4 cut(s) 289, 780, 858, 1180
NmuCI GTSAC 1 cut(s) 1074
NspI RCATGY 1 cut(s) 429
PaeR7I CTCGAG 1 cut(s) 810
PauI GCGCGC 1 cut(s) 73
PceI AGGCCT 1 cut(s) 973
PctI GAATGC 1 cut(s) 1049
PfeI GAWTC 3 cut(s) 159, 541, 1239
PflFI GACNNNGTC 1 cut(s) 650
PflMI CCANNNNNTGG 1 cut(s) 803
PkrI GCNGC 4 cut(s) 176, 412, 678, 1150
PleI GAGTC 3 cut(s) 147, 254, 929
PluTI GGCGCC 1 cut(s) 860
PmaCI CACGTG 1 cut(s) 695
PmlI CACGTG 1 cut(s) 695
PpsI GAGTC 3 cut(s) 147, 254, 929
Ppu21I YACGTR 1 cut(s) 695
PpuMI RGGWCCY 1 cut(s) 778
Psp124BI GAGCTC 1 cut(s) 811
Psp5II RGGWCCY 1 cut(s) 778
Psp6I CCWGG 5 cut(s) 270, 389, 943, 1080, 1116
PspCI CACGTG 1 cut(s) 695
PspGI CCWGG 5 cut(s) 270, 389, 943, 1080, 1116
PspN4I GGNNCC 4 cut(s) 289, 780, 858, 1180
PspPI GGNCC 2 cut(s) 346, 778
PspPPI RGGWCCY 1 cut(s) 778
PspXI VCTCGAGB 1 cut(s) 810
PsyI GACNNNGTC 1 cut(s) 650
PteI GCGCGC 1 cut(s) 73
RseI CAYNNNNRTG 2 cut(s) 474, 1042
SacI GAGCTC 1 cut(s) 811
SaqAI TTAA 5 cut(s) 503, 531, 549, 714, 1071
SatI GCNGC 4 cut(s) 175, 411, 677, 1149
Sau3AI GATC 3 cut(s) 667, 865, 1099
Sau96I GGNCC 2 cut(s) 346, 778
SchI GAGTC 3 cut(s) 148, 255, 929
ScrFI CCNGG 5 cut(s) 272, 391, 945, 1082, 1118
SduI GDGCHC 4 cut(s) 481, 769, 811, 1067
SfaNI GCATC 4 cut(s) 155, 364, 458, 1135
SfcI CTRYAG 1 cut(s) 510
SfoI GGCGCC 1 cut(s) 858
Sfr274I CTCGAG 1 cut(s) 810
SinI GGWCC 2 cut(s) 346, 778
SlaI CTCGAG 1 cut(s) 810
SmiMI CAYNNNNRTG 2 cut(s) 474, 1042
SmlI CTYRAG 1 cut(s) 810
SmoI CTYRAG 1 cut(s) 810
Sse9I AATT 7 cut(s) 127, 277, 383, 718, 746, 953, 1164
SseBI AGGCCT 1 cut(s) 973
SsiI CCGC 5 cut(s) 77, 153, 569, 874, 1246
SspDI GGCGCC 1 cut(s) 856
SspMI CTAG 1 cut(s) 111
SstI GAGCTC 1 cut(s) 811
StuI AGGCCT 1 cut(s) 973
StyD4I CCNGG 5 cut(s) 270, 389, 943, 1080, 1116
TaaI ACNGT 2 cut(s) 223, 369
TaiI ACGT 1 cut(s) 697
TaqI TCGA 2 cut(s) 443, 811
TasI AATT 7 cut(s) 127, 277, 383, 718, 746, 953, 1164
TfiI GAWTC 3 cut(s) 159, 541, 1239
Tru1I TTAA 5 cut(s) 503, 531, 549, 714, 1071
Tru9I TTAA 5 cut(s) 503, 531, 549, 714, 1071
TscAI CASTG 2 cut(s) 75, 226
TseFI GTSAC 1 cut(s) 1074
TseI GCWGC 4 cut(s) 174, 410, 676, 1148
Tsp45I GTSAC 1 cut(s) 1074
TspDTI ATGAA 3 cut(s) 328, 591, 1070
TspGWI ACGGA 1 cut(s) 748
TspRI CASTG 2 cut(s) 75, 226
Tth111I GACNNNGTC 1 cut(s) 650
Van91I CCANNNNNTGG 1 cut(s) 803
VneI GTGCAC 1 cut(s) 765
VpaK11BI GGWCC 2 cut(s) 346, 778
XapI RAATTY 2 cut(s) 953, 1164
XceI RCATGY 1 cut(s) 429
XhoI CTCGAG 1 cut(s) 810
XspI CTAG 1 cut(s) 111
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.