Rh7AG499100

protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0001220
Physical Location & Seq
Reverse (-)
69910442 .. 69914249
3808 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG499100.1

Sequence Viewer

Length: 873 bp
ATGCGGAGAATGCCAATGCTACGACGGACAAGGACTATCCGAGCGGCGAGTCTCTGGAAGAGCTTCGTCGTCAAGAAGGGAAGCGTCTTCACCGTGACATTGCGTGGCCAGATAACCGATCAGTTAAAGAGTCGAATTGCTGGTGTGTATCTTCAAATTGAGCGCTTGAACTGTGGATGGGGAAAAAAGAGAGAAGATATTGAGAAGTTTGTTAATGAAGGGGTGTACCAAGTAGAAAAGCTGAAAGAAGAGGGCTGGATCACAAACATACAGTATGATGATGAGGTTACTTCAATGTTGAAGGAAAGACTTGGAGTACAAAAAGAGAAAAAGCTTCCTATGGTTGATTACAGGAAATATTCTAAGGTCCGGAAATGGACTCTTGGTTTATCAGGCGAACAGTTCATTGAGAAGATCCGTACTATAAGAGAATCAAAAAGATACAAGGCTGCTATTATCCGAATTGACAGCCCTGGAGGATGTGGAGGGAAATCAATCTTTTGGCTGCATCAAAACCAGTCATTGCATCAATGTCTGATGTGGCAGCAAGTGGAGGATACTATGGCTATGGCAGCAGATGCCATTGTTGCAGAAAATCTAACCTTAACTGGTTCCATTGGAGTTGTCACAGGGAAGTTTAACCTTGGAAAACTGTGTGAGAAGATTGGCTTCAACAAAGAAATCATATCACGGGGAAAATTTGCAGAGGTTCTTGCAGCTGAACAACGACCATTCAGACCAGAGGAAGCTGAACTTTTTGCCAAGTCTGCACAGAATTCGTATAAGCAATTTCGGGGCAAGGCAGCTTCTTCCAGATCAATGACTGTAGATAAAATGGAGGAAGTTGCACAAGGGAGAGTGAGTCTAGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_S49

Protein Analysis

290

Amino Acids

33.2

Weight (kDa)

9.78

Isoelectric Point (pI)

37.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S49 PF01343 188 - 287 9.6e-21 Peptidase family S49
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 44
AccIII TCCGGA 1 cut(s) 369
AciI CCGC 2 cut(s) 4, 44
AclWI GGATC 2 cut(s) 266, 409
AcoI YGGCCR 1 cut(s) 106
AcsI RAATTY 2 cut(s) 698, 775
AfaI GTAC 3 cut(s) 227, 318, 421
AfeI AGCGCT 1 cut(s) 164
AgsI TTSAA 5 cut(s) 155, 169, 294, 301, 673
AjnI CCWGG 1 cut(s) 472
AluBI AGCT 6 cut(s) 63, 241, 334, 719, 749, 806
AluI AGCT 6 cut(s) 63, 241, 334, 719, 749, 806
Alw26I GTCTC 1 cut(s) 56
AlwI GGATC 2 cut(s) 266, 409
Aor13HI TCCGGA 1 cut(s) 369
Aor51HI AGCGCT 1 cut(s) 164
AoxI GGCC 1 cut(s) 106
ApeKI GCWGC 6 cut(s) 449, 505, 544, 572, 716, 803
ApoI RAATTY 2 cut(s) 698, 775
Asp700I GAANNNNTTC 1 cut(s) 62
AspLEI GCGC 1 cut(s) 165
AspS9I GGNCC 1 cut(s) 367
AsuHPI GGTGA 1 cut(s) 82
AvaII GGWCC 1 cut(s) 367
BalI TGGCCA 1 cut(s) 108
BbsI GAAGAC 1 cut(s) 79
BbvI GCAGC 6 cut(s) 436, 492, 556, 584, 728, 815
BccI CCATC 1 cut(s) 171
BcgI CGANNNNNNTGC 2 cut(s) 759, 793
BciT130I CCWGG 1 cut(s) 474
BciVI GTATCC 1 cut(s) 550
BcoDI GTCTC 1 cut(s) 56
BfaI CTAG 1 cut(s) 866
BfmI CTRYAG 1 cut(s) 825
BfoI RGCGCY 1 cut(s) 166
BfuI GTATCC 1 cut(s) 550
BisI GCNGC 7 cut(s) 45, 450, 506, 545, 573, 717, 804
BlsI GCNGC 7 cut(s) 46, 451, 507, 546, 574, 718, 805
Bme1390I CCNGG 1 cut(s) 474
Bme18I GGWCC 1 cut(s) 367
BmgT120I GGNCC 1 cut(s) 367
BmiI GGNNCC 1 cut(s) 613
BmrFI CCNGG 1 cut(s) 474
BmsI GCATC 3 cut(s) 517, 535, 568
BpiI GAAGAC 1 cut(s) 79
BpmI CTGGAG 1 cut(s) 495
BsaJI CCNNGG 2 cut(s) 472, 643
BsaWI WCCGGW 1 cut(s) 369
BsaXI ACNNNNNCTCC 1 cut(s) 847
Bse1I ACTGG 2 cut(s) 517, 613
Bse3DI GCAATG 2 cut(s) 98, 521
BseAI TCCGGA 1 cut(s) 369
BseBI CCWGG 1 cut(s) 474
BseDI CCNNGG 2 cut(s) 472, 643
BseGI GGATG 2 cut(s) 182, 485
BseMI GCAATG 2 cut(s) 98, 521
BseNI ACTGG 2 cut(s) 517, 613
BseXI GCAGC 6 cut(s) 436, 492, 556, 584, 728, 815
BsgI GTGCAG 1 cut(s) 753
BshFI GGCC 1 cut(s) 108
BsiSI CCGG 1 cut(s) 370
BsmAI GTCTC 1 cut(s) 56
BsmI GAATGC 1 cut(s) 15
BsnI GGCC 1 cut(s) 108
Bsp13I TCCGGA 1 cut(s) 369
Bsp143I GATC 4 cut(s) 118, 258, 414, 815
BspACI CCGC 2 cut(s) 4, 44
BspANI GGCC 1 cut(s) 108
BspEI TCCGGA 1 cut(s) 369
BspLI GGNNCC 1 cut(s) 613
BspPI GGATC 2 cut(s) 266, 409
BspQI GCTCTTC 1 cut(s) 53
BsrBI CCGCTC 1 cut(s) 44
BsrDI GCAATG 2 cut(s) 98, 521
BsrI ACTGG 2 cut(s) 517, 613
BssECI CCNNGG 2 cut(s) 472, 643
BssMI GATC 4 cut(s) 118, 258, 414, 815
BssT1I CCWWGG 1 cut(s) 643
Bst2UI CCWGG 1 cut(s) 474
Bst4CI ACNGT 6 cut(s) 94, 173, 273, 402, 654, 826
Bst6I CTCTTC 2 cut(s) 53, 243
BstAPI GCANNNNNTGC 1 cut(s) 578
BstDEI CTNAG 1 cut(s) 363
BstF5I GGATG 2 cut(s) 182, 485
BstH2I RGCGCY 1 cut(s) 166
BstHHI GCGC 1 cut(s) 165
BstKTI GATC 4 cut(s) 121, 261, 417, 818
BstMAI GTCTC 1 cut(s) 56
BstMBI GATC 4 cut(s) 118, 258, 414, 815
BstMWI GCNNNNNNNGC 5 cut(s) 10, 572, 578, 587, 767
BstNI CCWGG 1 cut(s) 474
BstSCI CCNGG 1 cut(s) 472
BstSFI CTRYAG 1 cut(s) 825
BstV1I GCAGC 6 cut(s) 436, 492, 556, 584, 728, 815
BstV2I GAAGAC 1 cut(s) 79
BstX2I RGATCY 1 cut(s) 414
BstYI RGATCY 1 cut(s) 414
BsuI GTATCC 1 cut(s) 550
BsuRI GGCC 1 cut(s) 108
BtsCI GGATG 2 cut(s) 182, 485
CfoI GCGC 1 cut(s) 165
Cfr13I GGNCC 1 cut(s) 367
CseI GACGC 1 cut(s) 73
Csp6I GTAC 3 cut(s) 226, 317, 420
CviQI GTAC 3 cut(s) 226, 317, 420
DdeI CTNAG 1 cut(s) 363
DpnI GATC 4 cut(s) 120, 260, 416, 817
DpnII GATC 4 cut(s) 118, 258, 414, 815
EaeI YGGCCR 1 cut(s) 106
Eam1104I CTCTTC 2 cut(s) 53, 243
EarI CTCTTC 2 cut(s) 53, 243
Eco130I CCWWGG 1 cut(s) 643
Eco47I GGWCC 1 cut(s) 367
Eco47III AGCGCT 1 cut(s) 164
EcoRI GAATTC 1 cut(s) 775
EcoRII CCWGG 1 cut(s) 472
EcoT14I CCWWGG 1 cut(s) 643
ErhI CCWWGG 1 cut(s) 643
FaiI YATR 8 cut(s) 269, 276, 341, 425, 563, 569, 686, 783
FalI AAGNNNNNCTT 4 cut(s) 653, 685, 738, 770
Fnu4HI GCNGC 7 cut(s) 45, 450, 506, 545, 573, 717, 804
FokI GGATG 2 cut(s) 189, 492
Fsp4HI GCNGC 7 cut(s) 45, 450, 506, 545, 573, 717, 804
FspBI CTAG 1 cut(s) 866
GlaI GCGC 1 cut(s) 164
GluI GCNGC 7 cut(s) 45, 450, 506, 545, 573, 717, 804
GsuI CTGGAG 1 cut(s) 495
HaeII RGCGCY 1 cut(s) 166
HaeIII GGCC 1 cut(s) 108
HapII CCGG 1 cut(s) 370
HgaI GACGC 1 cut(s) 73
HhaI GCGC 1 cut(s) 165
Hin6I GCGC 1 cut(s) 163
HinP1I GCGC 1 cut(s) 163
HindIII AAGCTT 1 cut(s) 332
HinfI GANTC 5 cut(s) 49, 130, 379, 431, 862
HpaII CCGG 1 cut(s) 370
HphI GGTGA 1 cut(s) 82
Hpy166II GTNNAC 1 cut(s) 226
Hpy188I TCNGA 4 cut(s) 41, 461, 537, 737
Hpy188III TCNNGA 5 cut(s) 55, 73, 370, 813, 866
Hpy8I GTNNAC 1 cut(s) 226
Hpy99I CGWCG 2 cut(s) 27, 71
HpyAV CCTTC 3 cut(s) 70, 212, 295
HpyCH4III ACNGT 6 cut(s) 94, 173, 273, 402, 654, 826
HpyCH4V TGCA 7 cut(s) 508, 526, 590, 704, 716, 770, 848
HpyF10VI GCNNNNNNNGC 5 cut(s) 10, 572, 578, 587, 767
HpyF3I CTNAG 1 cut(s) 363
HspAI GCGC 1 cut(s) 163
Kpn2I TCCGGA 1 cut(s) 369
Kzo9I GATC 4 cut(s) 118, 258, 414, 815
LguI GCTCTTC 1 cut(s) 53
Lsp1109I GCAGC 6 cut(s) 436, 492, 556, 584, 728, 815
LweI GCATC 3 cut(s) 517, 535, 568
MaeI CTAG 1 cut(s) 866
MaeIII GTNAC 3 cut(s) 94, 286, 625
MalI GATC 4 cut(s) 120, 260, 416, 817
MbiI CCGCTC 1 cut(s) 44
MboI GATC 4 cut(s) 118, 258, 414, 815
MboII GAAGA 8 cut(s) 70, 79, 143, 206, 260, 424, 673, 801
MflI RGATCY 1 cut(s) 414
MlsI TGGCCA 1 cut(s) 108
MluCI AATT 6 cut(s) 135, 156, 462, 698, 775, 788
MluNI TGGCCA 1 cut(s) 108
MlyI GAGTC 4 cut(s) 58, 139, 373, 871
MnlI CCTC 8 cut(s) 244, 277, 470, 479, 547, 700, 736, 832
Mox20I TGGCCA 1 cut(s) 108
MroI TCCGGA 1 cut(s) 369
MroXI GAANNNNTTC 1 cut(s) 62
MscI TGGCCA 1 cut(s) 108
MseI TTAA 4 cut(s) 125, 213, 605, 639
Msp20I TGGCCA 1 cut(s) 108
MspA1I CMGCKG 1 cut(s) 719
MspI CCGG 1 cut(s) 370
MspR9I CCNGG 1 cut(s) 474
Mva1269I GAATGC 1 cut(s) 15
MvaI CCWGG 1 cut(s) 474
MwoI GCNNNNNNNGC 5 cut(s) 10, 572, 578, 587, 767
NdeII GATC 4 cut(s) 118, 258, 414, 815
NlaIV GGNNCC 1 cut(s) 613
NmuCI GTSAC 2 cut(s) 94, 625
PciSI GCTCTTC 1 cut(s) 53
PctI GAATGC 1 cut(s) 15
PdmI GAANNNNTTC 1 cut(s) 62
PfeI GAWTC 1 cut(s) 431
PkrI GCNGC 7 cut(s) 46, 451, 507, 546, 574, 718, 805
PleI GAGTC 4 cut(s) 57, 138, 373, 870
PpsI GAGTC 4 cut(s) 57, 138, 373, 870
Psp6I CCWGG 1 cut(s) 472
PspGI CCWGG 1 cut(s) 472
PspN4I GGNNCC 1 cut(s) 613
PspPI GGNCC 1 cut(s) 367
PsuI RGATCY 1 cut(s) 414
PvuII CAGCTG 1 cut(s) 719
RsaI GTAC 3 cut(s) 227, 318, 421
RsaNI GTAC 3 cut(s) 226, 317, 420
SapI GCTCTTC 1 cut(s) 53
SaqAI TTAA 4 cut(s) 125, 213, 605, 639
SatI GCNGC 7 cut(s) 45, 450, 506, 545, 573, 717, 804
Sau3AI GATC 4 cut(s) 118, 258, 414, 815
Sau96I GGNCC 1 cut(s) 367
SchI GAGTC 4 cut(s) 58, 139, 373, 871
ScrFI CCNGG 1 cut(s) 474
SfaNI GCATC 3 cut(s) 517, 535, 568
SfcI CTRYAG 1 cut(s) 825
SinI GGWCC 1 cut(s) 367
Sse9I AATT 6 cut(s) 135, 156, 462, 698, 775, 788
SsiI CCGC 2 cut(s) 4, 44
SspI AATATT 1 cut(s) 359
SspMI CTAG 1 cut(s) 866
StyD4I CCNGG 1 cut(s) 472
StyI CCWWGG 1 cut(s) 643
TaaI ACNGT 6 cut(s) 94, 173, 273, 402, 654, 826
TaqI TCGA 1 cut(s) 133
TasI AATT 6 cut(s) 135, 156, 462, 698, 775, 788
TatI WGTACW 1 cut(s) 316
TauI GCSGC 1 cut(s) 47
TfiI GAWTC 1 cut(s) 431
Tru1I TTAA 4 cut(s) 125, 213, 605, 639
Tru9I TTAA 4 cut(s) 125, 213, 605, 639
TseFI GTSAC 2 cut(s) 94, 625
TseI GCWGC 6 cut(s) 449, 505, 544, 572, 716, 803
Tsp45I GTSAC 2 cut(s) 94, 625
TspDTI ATGAA 2 cut(s) 231, 394
TspGWI ACGGA 2 cut(s) 40, 407
VpaK11BI GGWCC 1 cut(s) 367
XapI RAATTY 2 cut(s) 698, 775
XbaI TCTAGA 1 cut(s) 865
XmnI GAANNNNTTC 1 cut(s) 62
XspI CTAG 1 cut(s) 866
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.