Rh7AG063000

protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0001220
Physical Location & Seq
Reverse (-)
4388333 .. 4391133
2801 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG063000.1

Sequence Viewer

Length: 639 bp
ATGTTGAAGGAAAGACTTGGAGTACAAAAAGAGAAAAAGCTTCCTATGGTTGATTACAGGATGTGGAGGGAAATCAAACTTTTGGCTGCATCAAAGCCGGTCATTGCATCAATGTCTGATGTGGCAGCAAGTGGAGGATACTATATGGCTATGGCAGCAGATGCCATTGTTGCAGAAAATCTAACCTTAACTGGTTCCATTGGAGTTGTCACAGGGAAGTTTAACCTGGGAAAACTGTATGAGATGATTGGCTTCAACAAGGAAATCATATCAAGGGGGAAATTTGCAGAGGTTCTTGCAGCTGAACAACGACCCTTCAAACCAGAGGAAGCTGAACTCTTTGCTAAGTCTGCACAGAATTCGTATAAGCAATTTCGGGACAAGGCAGCTTCTTCCAGATCAATGACTCAAGTAAGAATGGAAGCAATTACAAATCAAGATTGTGAGCCAGCAGAGCGCATGGATGAAGAATGCGACACATTTGAACCAGAGAGCGGGAAATGGAATAGTGATGGATATGAAGATTGCAGTGACCCCAAAAAAAATGCCTACTCGCTCGAGCACGTGAGCCGCTTGTTCGAAAATGGAGCGAATACATCTGCCTACAAAGCAGCAAGTAAAGGCAAGTATGATGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_S49

Protein Analysis

212

Amino Acids

23.59

Weight (kDa)

5.69

Isoelectric Point (pI)

29.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S49 PF01343 28 - 146 1.6e-29 Peptidase family S49
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 495
AciI CCGC 2 cut(s) 495, 571
AcsI RAATTY 2 cut(s) 281, 358
AcvI CACGTG 1 cut(s) 565
AfaI GTAC 1 cut(s) 24
AfiI CCNNNNNNNGG 1 cut(s) 494
AgsI TTSAA 4 cut(s) 7, 256, 319, 485
AjnI CCWGG 1 cut(s) 225
AluBI AGCT 4 cut(s) 40, 302, 332, 389
AluI AGCT 4 cut(s) 40, 302, 332, 389
Alw21I GWGCWC 1 cut(s) 564
Ama87I CYCGRG 1 cut(s) 557
ApeKI GCWGC 6 cut(s) 86, 125, 155, 299, 386, 611
ApoI RAATTY 2 cut(s) 281, 358
AspLEI GCGC 1 cut(s) 459
AsuII TTCGAA 1 cut(s) 579
AvaI CYCGRG 1 cut(s) 557
BbrPI CACGTG 1 cut(s) 565
Bbv12I GWGCWC 1 cut(s) 564
BbvI GCAGC 6 cut(s) 73, 137, 167, 311, 398, 623
BccI CCATC 2 cut(s) 506, 626
BcgI CGANNNNNNTGC 2 cut(s) 342, 376
BciT130I CCWGG 1 cut(s) 227
BciVI GTATCC 1 cut(s) 131
BfuI GTATCC 1 cut(s) 131
BisI GCNGC 7 cut(s) 87, 126, 156, 300, 387, 571, 612
BlsI GCNGC 7 cut(s) 88, 127, 157, 301, 388, 572, 613
Bme1390I CCNGG 1 cut(s) 227
BmeT110I CYCGRG 1 cut(s) 557
BmiI GGNNCC 1 cut(s) 196
BmrFI CCNGG 1 cut(s) 227
BmsI GCATC 3 cut(s) 98, 116, 151
Bpu14I TTCGAA 1 cut(s) 579
BpuEI CTTGAG 1 cut(s) 393
BsaAI YACGTR 1 cut(s) 565
BsaJI CCNNGG 1 cut(s) 226
Bsc4I CCNNNNNNNGG 1 cut(s) 494
Bse118I RCCGGY 1 cut(s) 97
Bse1I ACTGG 1 cut(s) 196
Bse3DI GCAATG 1 cut(s) 102
BseBI CCWGG 1 cut(s) 227
BseDI CCNNGG 1 cut(s) 226
BseGI GGATG 2 cut(s) 66, 469
BseLI CCNNNNNNNGG 1 cut(s) 494
BseMI GCAATG 1 cut(s) 102
BseNI ACTGG 1 cut(s) 196
BseXI GCAGC 6 cut(s) 73, 137, 167, 311, 398, 623
BsgI GTGCAG 1 cut(s) 336
BsiHKAI GWGCWC 1 cut(s) 564
BsiHKCI CYCGRG 1 cut(s) 557
BsiSI CCGG 1 cut(s) 98
BslFI GGGAC 1 cut(s) 392
BslI CCNNNNNNNGG 1 cut(s) 494
BsmFI GGGAC 1 cut(s) 392
BsmI GAATGC 1 cut(s) 476
BsoBI CYCGRG 1 cut(s) 557
Bsp119I TTCGAA 1 cut(s) 579
Bsp1286I GDGCHC 1 cut(s) 564
Bsp143I GATC 1 cut(s) 398
BspACI CCGC 2 cut(s) 495, 571
BspLI GGNNCC 1 cut(s) 196
BspT104I TTCGAA 1 cut(s) 579
BsrBI CCGCTC 1 cut(s) 495
BsrDI GCAATG 1 cut(s) 102
BsrFI RCCGGY 1 cut(s) 97
BsrI ACTGG 1 cut(s) 196
BssAI RCCGGY 1 cut(s) 97
BssECI CCNNGG 1 cut(s) 226
BssMI GATC 1 cut(s) 398
Bst2UI CCWGG 1 cut(s) 227
Bst4CI ACNGT 1 cut(s) 237
BstAPI GCANNNNNTGC 1 cut(s) 161
BstBAI YACGTR 1 cut(s) 565
BstBI TTCGAA 1 cut(s) 579
BstC8I GCNNGC 1 cut(s) 450
BstDEI CTNAG 1 cut(s) 345
BstF5I GGATG 2 cut(s) 66, 469
BstHHI GCGC 1 cut(s) 459
BstKTI GATC 1 cut(s) 401
BstMBI GATC 1 cut(s) 398
BstMWI GCNNNNNNNGC 6 cut(s) 155, 161, 170, 350, 454, 608
BstNI CCWGG 1 cut(s) 227
BstSCI CCNGG 1 cut(s) 225
BstV1I GCAGC 6 cut(s) 73, 137, 167, 311, 398, 623
BsuI GTATCC 1 cut(s) 131
BtsCI GGATG 2 cut(s) 66, 469
BtsI GCAGTG 1 cut(s) 535
BtsIMutI CAGTG 1 cut(s) 535
Cac8I GCNNGC 1 cut(s) 450
CfoI GCGC 1 cut(s) 459
Cfr10I RCCGGY 1 cut(s) 97
Csp6I GTAC 1 cut(s) 23
CviAII CATG 1 cut(s) 460
CviQI GTAC 1 cut(s) 23
DdeI CTNAG 1 cut(s) 345
DpnI GATC 1 cut(s) 400
DpnII GATC 1 cut(s) 398
Eco72I CACGTG 1 cut(s) 565
Eco88I CYCGRG 1 cut(s) 557
EcoRI GAATTC 1 cut(s) 358
EcoRII CCWGG 1 cut(s) 225
FaeI CATG 1 cut(s) 463
FaqI GGGAC 1 cut(s) 392
FatI CATG 1 cut(s) 459
FauI CCCGC 1 cut(s) 488
Fnu4HI GCNGC 7 cut(s) 87, 126, 156, 300, 387, 571, 612
FokI GGATG 2 cut(s) 73, 476
Fsp4HI GCNGC 7 cut(s) 87, 126, 156, 300, 387, 571, 612
GlaI GCGC 1 cut(s) 458
GluI GCNGC 7 cut(s) 87, 126, 156, 300, 387, 571, 612
HapII CCGG 1 cut(s) 98
HhaI GCGC 1 cut(s) 459
Hin1II CATG 1 cut(s) 463
Hin6I GCGC 1 cut(s) 457
HinP1I GCGC 1 cut(s) 457
HindIII AAGCTT 1 cut(s) 38
HinfI GANTC 1 cut(s) 406
HpaII CCGG 1 cut(s) 98
Hpy188I TCNGA 1 cut(s) 118
Hpy188III TCNNGA 3 cut(s) 377, 396, 437
HpyAV CCTTC 1 cut(s) 325
HpyCH4III ACNGT 1 cut(s) 237
HpyCH4IV ACGT 1 cut(s) 564
HpyCH4V TGCA 7 cut(s) 89, 107, 173, 287, 299, 353, 528
HpyF10VI GCNNNNNNNGC 6 cut(s) 155, 161, 170, 350, 454, 608
HpyF3I CTNAG 1 cut(s) 345
HpySE526I ACGT 1 cut(s) 564
Hsp92II CATG 1 cut(s) 463
HspAI GCGC 1 cut(s) 457
Kzo9I GATC 1 cut(s) 398
LmnI GCTCC 1 cut(s) 587
Lsp1109I GCAGC 6 cut(s) 73, 137, 167, 311, 398, 623
LweI GCATC 3 cut(s) 98, 116, 151
MaeII ACGT 1 cut(s) 564
MaeIII GTNAC 2 cut(s) 208, 530
MalI GATC 1 cut(s) 400
MbiI CCGCTC 1 cut(s) 495
MboI GATC 1 cut(s) 398
MboII GAAGA 3 cut(s) 384, 479, 533
MhlI GDGCHC 1 cut(s) 564
MluCI AATT 4 cut(s) 281, 358, 371, 426
MlyI GAGTC 1 cut(s) 400
MnlI CCTC 4 cut(s) 60, 128, 283, 319
MseI TTAA 3 cut(s) 188, 222, 637
MspA1I CMGCKG 1 cut(s) 302
MspI CCGG 1 cut(s) 98
MspR9I CCNGG 1 cut(s) 227
Mva1269I GAATGC 1 cut(s) 476
MvaI CCWGG 1 cut(s) 227
MwoI GCNNNNNNNGC 6 cut(s) 155, 161, 170, 350, 454, 608
NdeII GATC 1 cut(s) 398
NlaIII CATG 1 cut(s) 463
NlaIV GGNNCC 1 cut(s) 196
NmuCI GTSAC 2 cut(s) 208, 530
NspV TTCGAA 1 cut(s) 579
PaeR7I CTCGAG 1 cut(s) 557
PctI GAATGC 1 cut(s) 476
PkrI GCNGC 7 cut(s) 88, 127, 157, 301, 388, 572, 613
PleI GAGTC 1 cut(s) 400
PmaCI CACGTG 1 cut(s) 565
PmlI CACGTG 1 cut(s) 565
PpsI GAGTC 1 cut(s) 400
Ppu21I YACGTR 1 cut(s) 565
Psp6I CCWGG 1 cut(s) 225
PspCI CACGTG 1 cut(s) 565
PspGI CCWGG 1 cut(s) 225
PspN4I GGNNCC 1 cut(s) 196
PspXI VCTCGAGB 1 cut(s) 557
PvuII CAGCTG 1 cut(s) 302
RsaI GTAC 1 cut(s) 24
RsaNI GTAC 1 cut(s) 23
SaqAI TTAA 3 cut(s) 188, 222, 637
SatI GCNGC 7 cut(s) 87, 126, 156, 300, 387, 571, 612
Sau3AI GATC 1 cut(s) 398
SchI GAGTC 1 cut(s) 400
ScrFI CCNGG 1 cut(s) 227
SduI GDGCHC 1 cut(s) 564
SetI ASST 8 cut(s) 42, 188, 228, 294, 304, 334, 391, 567
SfaNI GCATC 3 cut(s) 98, 116, 151
Sfr274I CTCGAG 1 cut(s) 557
SfuI TTCGAA 1 cut(s) 579
SlaI CTCGAG 1 cut(s) 557
SmlI CTYRAG 2 cut(s) 408, 557
SmoI CTYRAG 2 cut(s) 408, 557
Sse9I AATT 4 cut(s) 281, 358, 371, 426
SsiI CCGC 2 cut(s) 495, 571
StyD4I CCNGG 1 cut(s) 225
TaaI ACNGT 1 cut(s) 237
TaiI ACGT 1 cut(s) 567
TaqI TCGA 2 cut(s) 558, 579
TasI AATT 4 cut(s) 281, 358, 371, 426
TatI WGTACW 1 cut(s) 22
TauI GCSGC 1 cut(s) 573
Tru1I TTAA 3 cut(s) 188, 222, 637
Tru9I TTAA 3 cut(s) 188, 222, 637
TscAI CASTG 1 cut(s) 535
TseFI GTSAC 2 cut(s) 208, 530
TseI GCWGC 6 cut(s) 86, 125, 155, 299, 386, 611
Tsp45I GTSAC 2 cut(s) 208, 530
TspDTI ATGAA 2 cut(s) 480, 534
TspRI CASTG 1 cut(s) 535
XapI RAATTY 2 cut(s) 281, 358
XhoI CTCGAG 1 cut(s) 557
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.