Rmu_sc0004712.1_g000014

protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004712.1
OG0001220
Physical Location & Seq
Forward (+)
61618 .. 65518
3901 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0004712.1_g000014.1.cds

Sequence Viewer

Length: 2052 bp
atgtcgaagctgattctcctccaggcttcccaccacctcaccccaatccacacccgagccttcaccgccgtcgtcggagcctctctctccacgccgctcctccactctcactgccgtcctctcttctctcactctcctcctcctctctctctccgccgccgcctctccctacgcgccttcgacgactcgtcgtccgccaccaaaccagatgaaacgaccgaaaacggcgccgtttcagctcccgaggctaaaatcgacgccgagaatgccaaagctaagacggacaaggactatccgagcggcgagttccagtttcaggaggcgagcctctggaagagcttcgtcgttaagcttcggatgctctttgcttatccttgggagcgggtcaagaagggaagcgtcttgaccatgacattgcgcggccagataactgatcagttaaagagtcgattctctaggggactatcgctgcctcaaatttgtgagaattttgtgaaagctgcacatgatccccgaattgctggtgtgtatcttcaaattgagagcttgaactgtggatggggtaaagtggaagaaattcggaggcatatattggacttccagaaatcaggtaagtttgttgtggcatacgcacctgcttgtagcgaaaaagagtattaccttgcttctgcttgtcaggagatttatgcccctccaagtgcttatttttccttgtttggtctcagtgttcaggcatcatttgtaagaggtgttcttgagaaaattggagttgagccacaagtggaaaggattggtaaatataaaagtgctggtgaccaacttgcgcgcacgagcatgtccgaagaaaattgtgagatgctgacagcattgctcgataacatatatgggaactggctggacataatttcttctacaagaggaaaaaagagagaagatattgagaagtttgttaatgaaggggtgtaccaagtagaaaagctgaaagaagagggctggatcacaaacatacagtatgatgatgaggttacttcaatgttgaaggaaagacttggagtacaaaaagagaaaaagcttcctatggttgattacaggaaatattctaaggttcggaaatggactcttggtttatcaggcggtaaagacaagatagccataataagagcctcaggcagtattagtcgtgtacgaggttcttttagtctacctggatcatctattgttggagaacagttcattgagaagatccgtactataagagaatcaaaaagatacaaggctgctattatccgaattgacagccctggaggtgatgcccttgcttctgatttgatgtggagggaaatcaaacttttggctgcatcaaagccggtcattgcatcaatgtctgatgtggcagcaagtggaggatactatatggctatggcagcagatgccattgttgcagaaaatctaaccttaactggttccattggagttgtcacagggaagtttaacctgggaaaactgtatgagaagattggcttcaacaaggaaatcatatcaagggggaaatttgcggaggttcttgcagctgaacaacgacccttcaaaccagaggaagctgaactctttgctaagtctgcacagaattcgtataagcaatttcgggacaaggcagcttcttccagatcaatgactgcagataaaatggaggaagttgcacaagggagagtttgggctggtaaagatgcagcttcgaggggtttagttgatgccattggtggactttctcgggctgttgcaatagcaaagctcaaggcaaatataccacaagacacagaggttacacttgtggagcttgcaagaccctccccttcactgccagaacttttaagtggcgtagggagtactctggttggagtcgagcaaacgatgaagacactgttgcaagacttaacattttctgatggagttcaagcccggatggatggaatcttatttcagagactggagggagcttctaatgccaaccccatcttcaatttgataagagattaccttggttccctgtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_S49

Protein Analysis

683

Amino Acids

75.33

Weight (kDa)

9.17

Isoelectric Point (pI)

39.62

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 643
Acc36I ACCTGC 1 cut(s) 643
AccB1I GGYRCC 1 cut(s) 227
AccBSI CCGCTC 3 cut(s) 97, 300, 382
AccI GTMKAC 1 cut(s) 1201
AccII CGCG 3 cut(s) 174, 420, 826
AclWI GGATC 4 cut(s) 503, 1004, 1216, 1237
AcoI YGGCCR 1 cut(s) 421
AcsI RAATTY 5 cut(s) 477, 487, 576, 1550, 1627
AcyI GRCGYC 2 cut(s) 228, 258
AfaI GTAC 5 cut(s) 965, 1056, 1185, 1249, 1888
AfiI CCNNNNNNNGG 1 cut(s) 316
AgsI TTSAA 8 cut(s) 536, 550, 1032, 1039, 1525, 1588, 1955, 2020
AhdI GACNNNNNGTC 2 cut(s) 187, 190
AjnI CCWGG 4 cut(s) 21, 1204, 1300, 1494
AjuI GAANNNNNNNTTGG 2 cut(s) 2000, 2032
Alw26I GTCTC 2 cut(s) 725, 1978
AlwI GGATC 4 cut(s) 503, 1004, 1216, 1237
AlwNI CAGNNNCTG 1 cut(s) 1987
Ama87I CYCGRG 3 cut(s) 54, 242, 1770
AoxI GGCC 1 cut(s) 421
ApeKI GCWGC 9 cut(s) 469, 500, 1277, 1355, 1394, 1424, 1568, 1655, 1730
ApoI RAATTY 5 cut(s) 477, 487, 576, 1550, 1627
Asp700I GAANNNNTTC 2 cut(s) 338, 576
AspLEI GCGC 5 cut(s) 176, 230, 420, 826, 828
AsuC2I CCSGG 1 cut(s) 1960
AsuHPI GGTGA 4 cut(s) 31, 55, 824, 1319
AvaI CYCGRG 3 cut(s) 54, 242, 1770
AxyI CCTNAGG 1 cut(s) 1165
BanI GGYRCC 1 cut(s) 227
BauI CACGAG 1 cut(s) 829
BbsI GAAGAC 1 cut(s) 1922
BbvI GCAGC 9 cut(s) 456, 487, 1264, 1342, 1406, 1436, 1580, 1667, 1742
BccI CCATC 5 cut(s) 552, 1940, 1957, 1961, 2021
BceAI ACGGC 4 cut(s) 53, 99, 215, 241
BcgI CGANNNNNNTGC 2 cut(s) 1611, 1645
BciT130I CCWGG 4 cut(s) 23, 1206, 1302, 1496
BciVI GTATCC 1 cut(s) 1400
BclI TGATCA 1 cut(s) 433
BcnI CCSGG 1 cut(s) 1960
BcoDI GTCTC 2 cut(s) 725, 1978
BfaI CTAG 1 cut(s) 456
BfmI CTRYAG 1 cut(s) 1677
BfoI RGCGCY 1 cut(s) 231
BfuAI ACCTGC 1 cut(s) 643
BfuI GTATCC 1 cut(s) 1400
BmcAI AGTACT 1 cut(s) 1888
Bme1390I CCNGG 5 cut(s) 23, 1206, 1302, 1496, 1960
BmeRI GACNNNNNGTC 2 cut(s) 187, 190
BmeT110I CYCGRG 3 cut(s) 54, 242, 1770
BmiI GGNNCC 4 cut(s) 79, 229, 1465, 2044
BmrFI CCNGG 5 cut(s) 23, 1206, 1302, 1496, 1960
BmsI GCATC 9 cut(s) 348, 743, 846, 1300, 1367, 1385, 1420, 1717, 1741
BpiI GAAGAC 1 cut(s) 1922
BplI GAGNNNNNCTC 2 cut(s) 69, 101
BpmI CTGGAG 3 cut(s) 5, 1323, 2009
BpuEI CTTGAG 2 cut(s) 776, 1778
BpuMI CCSGG 1 cut(s) 1960
BsaHI GRCGYC 2 cut(s) 228, 258
BsaI GGTCTC 1 cut(s) 725
BsaJI CCNNGG 5 cut(s) 243, 374, 1300, 1495, 2038
Bsc4I CCNNNNNNNGG 1 cut(s) 316
Bse118I RCCGGY 1 cut(s) 1366
Bse1I ACTGG 4 cut(s) 310, 896, 1465, 1992
Bse21I CCTNAGG 1 cut(s) 1165
Bse3DI GCAATG 3 cut(s) 413, 866, 1371
BseBI CCWGG 4 cut(s) 23, 1206, 1302, 1496
BseDI CCNNGG 5 cut(s) 243, 374, 1300, 1495, 2038
BseGI GGATG 4 cut(s) 363, 563, 1968, 1972
BseLI CCNNNNNNNGG 1 cut(s) 316
BseMI GCAATG 3 cut(s) 413, 866, 1371
BseMII CTCAG 2 cut(s) 736, 1179
BseNI ACTGG 4 cut(s) 310, 896, 1465, 1992
BsePI GCGCGC 1 cut(s) 824
BseRI GAGGAG 5 cut(s) 8, 89, 126, 129, 132
BseXI GCAGC 9 cut(s) 456, 487, 1264, 1342, 1406, 1436, 1580, 1667, 1742
BsgI GTGCAG 2 cut(s) 486, 1605
Bsh1236I CGCG 3 cut(s) 174, 420, 826
Bsh1285I CGRYCG 1 cut(s) 219
BshFI GGCC 1 cut(s) 423
BshNI GGYRCC 1 cut(s) 227
BsiEI CGRYCG 1 cut(s) 219
BsiHKCI CYCGRG 3 cut(s) 54, 242, 1770
BsiSI CCGG 2 cut(s) 1367, 1960
BslFI GGGAC 2 cut(s) 474, 1661
BslI CCNNNNNNNGG 1 cut(s) 316
BsmAI GTCTC 2 cut(s) 725, 1978
BsmFI GGGAC 2 cut(s) 474, 1661
BsmI GAATGC 1 cut(s) 271
BsnI GGCC 1 cut(s) 423
Bso31I GGTCTC 1 cut(s) 725
BsoBI CYCGRG 3 cut(s) 54, 242, 1770
Bsp143I GATC 6 cut(s) 433, 508, 996, 1208, 1242, 1667
BspANI GGCC 1 cut(s) 423
BspCNI CTCAG 2 cut(s) 735, 1178
BspFNI CGCG 3 cut(s) 174, 420, 826
BspLI GGNNCC 4 cut(s) 79, 229, 1465, 2044
BspMAI CTGCAG 1 cut(s) 1681
BspMI ACCTGC 1 cut(s) 643
BspPI GGATC 4 cut(s) 503, 1004, 1216, 1237
BspQI GCTCTTC 1 cut(s) 329
BspT107I GGYRCC 1 cut(s) 227
BspTNI GGTCTC 1 cut(s) 725
BsrBI CCGCTC 3 cut(s) 97, 300, 382
BsrDI GCAATG 3 cut(s) 413, 866, 1371
BsrFI RCCGGY 1 cut(s) 1366
BsrI ACTGG 4 cut(s) 310, 896, 1465, 1992
BssAI RCCGGY 1 cut(s) 1366
BssECI CCNNGG 5 cut(s) 243, 374, 1300, 1495, 2038
BssHII GCGCGC 1 cut(s) 824
BssMI GATC 6 cut(s) 433, 508, 996, 1208, 1242, 1667
BssNI GRCGYC 2 cut(s) 228, 258
BssSI CACGAG 1 cut(s) 829
BssT1I CCWWGG 2 cut(s) 374, 2038
Bst2BI CACGAG 1 cut(s) 829
Bst2UI CCWGG 4 cut(s) 23, 1206, 1302, 1496
Bst4CI ACNGT 5 cut(s) 554, 1011, 1230, 1506, 1923
Bst6I CTCTTC 3 cut(s) 128, 329, 981
BstACI GRCGYC 2 cut(s) 228, 258
BstAPI GCANNNNNTGC 1 cut(s) 1430
BstC8I GCNNGC 3 cut(s) 325, 826, 1839
BstDEI CTNAG 5 cut(s) 276, 722, 1101, 1165, 1614
BstEII GGTNACC 1 cut(s) 812
BstF5I GGATG 4 cut(s) 363, 563, 1968, 1972
BstFNI CGCG 3 cut(s) 174, 420, 826
BstH2I RGCGCY 1 cut(s) 231
BstHHI GCGC 5 cut(s) 176, 230, 420, 826, 828
BstKTI GATC 6 cut(s) 436, 511, 999, 1211, 1245, 1670
BstMAI GTCTC 2 cut(s) 725, 1978
BstMBI GATC 6 cut(s) 433, 508, 996, 1208, 1242, 1667
BstMCI CGRYCG 1 cut(s) 219
BstNI CCWGG 4 cut(s) 23, 1206, 1302, 1496
BstNSI RCATGY 1 cut(s) 838
BstPI GGTNACC 1 cut(s) 812
BstSCI CCNGG 5 cut(s) 21, 1204, 1300, 1494, 1958
BstSFI CTRYAG 1 cut(s) 1677
BstUI CGCG 3 cut(s) 174, 420, 826
BstV1I GCAGC 9 cut(s) 456, 487, 1264, 1342, 1406, 1436, 1580, 1667, 1742
BstV2I GAAGAC 1 cut(s) 1922
BstX2I RGATCY 1 cut(s) 1242
BstYI RGATCY 1 cut(s) 1242
Bsu36I CCTNAGG 1 cut(s) 1165
BsuI GTATCC 1 cut(s) 1400
BsuRI GGCC 1 cut(s) 423
BtsCI GGATG 4 cut(s) 363, 563, 1968, 1972
BtsI GCAGTG 2 cut(s) 109, 1856
BtsIMutI CAGTG 4 cut(s) 109, 730, 1856, 1919
BveI ACCTGC 1 cut(s) 643
Cac8I GCNNGC 3 cut(s) 325, 826, 1839
CaiI CAGNNNCTG 1 cut(s) 1987
CfoI GCGC 5 cut(s) 176, 230, 420, 826, 828
Cfr10I RCCGGY 1 cut(s) 1366
CseI GACGC 2 cut(s) 266, 388
Csp6I GTAC 5 cut(s) 964, 1055, 1184, 1248, 1887
CviAII CATG 3 cut(s) 409, 506, 835
CviQI GTAC 5 cut(s) 964, 1055, 1184, 1248, 1887
DdeI CTNAG 5 cut(s) 276, 722, 1101, 1165, 1614
DinI GGCGCC 1 cut(s) 229
DpnI GATC 6 cut(s) 435, 510, 998, 1210, 1244, 1669
DpnII GATC 6 cut(s) 433, 508, 996, 1208, 1242, 1667
DriI GACNNNNNGTC 2 cut(s) 187, 190
EaeI YGGCCR 1 cut(s) 421
Eam1104I CTCTTC 3 cut(s) 128, 329, 981
Eam1105I GACNNNNNGTC 2 cut(s) 187, 190
EarI CTCTTC 3 cut(s) 128, 329, 981
EciI GGCGGA 2 cut(s) 143, 184
Eco130I CCWWGG 2 cut(s) 374, 2038
Eco31I GGTCTC 1 cut(s) 725
Eco81I CCTNAGG 1 cut(s) 1165
Eco88I CYCGRG 3 cut(s) 54, 242, 1770
Eco91I GGTNACC 1 cut(s) 812
EcoO65I GGTNACC 1 cut(s) 812
EcoRI GAATTC 1 cut(s) 1627
EcoRII CCWGG 4 cut(s) 21, 1204, 1300, 1494
EcoT14I CCWWGG 2 cut(s) 374, 2038
EgeI GGCGCC 1 cut(s) 229
EheI GGCGCC 1 cut(s) 229
ErhI CCWWGG 2 cut(s) 374, 2038
FaeI CATG 3 cut(s) 412, 509, 838
FalI AAGNNNNNCTT 2 cut(s) 1505, 1537
FaqI GGGAC 2 cut(s) 474, 1661
FatI CATG 3 cut(s) 408, 505, 834
FauI CCCGC 1 cut(s) 375
FbaI TGATCA 1 cut(s) 433
FblI GTMKAC 1 cut(s) 1201
FokI GGATG 4 cut(s) 370, 570, 1975, 1979
FspBI CTAG 1 cut(s) 456
GlaI GCGC 5 cut(s) 175, 229, 419, 825, 827
GsuI CTGGAG 3 cut(s) 5, 1323, 2009
HaeII RGCGCY 1 cut(s) 231
HaeIII GGCC 1 cut(s) 423
HapII CCGG 2 cut(s) 1367, 1960
HgaI GACGC 2 cut(s) 266, 388
HhaI GCGC 5 cut(s) 176, 230, 420, 826, 828
Hin1I GRCGYC 2 cut(s) 228, 258
Hin1II CATG 3 cut(s) 412, 509, 838
Hin6I GCGC 5 cut(s) 174, 228, 418, 824, 826
HinP1I GCGC 5 cut(s) 174, 228, 418, 824, 826
HindIII AAGCTT 2 cut(s) 350, 1070
HinfI GANTC 8 cut(s) 13, 185, 445, 450, 1117, 1259, 1899, 1971
HpaII CCGG 2 cut(s) 1367, 1960
HphI GGTGA 4 cut(s) 31, 55, 824, 1319
Hpy166II GTNNAC 4 cut(s) 964, 1184, 1202, 1763
Hpy8I GTNNAC 4 cut(s) 964, 1184, 1202, 1763
Hpy99I CGWCG 6 cut(s) 74, 77, 185, 193, 260, 347
HpyAV CCTTC 7 cut(s) 70, 187, 385, 950, 1033, 1594, 1863
HpyCH4III ACNGT 5 cut(s) 554, 1011, 1230, 1506, 1923
HpyF3I CTNAG 5 cut(s) 276, 722, 1101, 1165, 1614
Hsp92I GRCGYC 2 cut(s) 228, 258
Hsp92II CATG 3 cut(s) 412, 509, 838
HspAI GCGC 5 cut(s) 174, 228, 418, 824, 826
KasI GGCGCC 1 cut(s) 227
Ksp22I TGATCA 1 cut(s) 433
Kzo9I GATC 6 cut(s) 433, 508, 996, 1208, 1242, 1667
LguI GCTCTTC 1 cut(s) 329
LmnI GCTCC 6 cut(s) 77, 102, 244, 379, 1834, 1994
Lsp1109I GCAGC 9 cut(s) 456, 487, 1264, 1342, 1406, 1436, 1580, 1667, 1742
LweI GCATC 9 cut(s) 348, 743, 846, 1300, 1367, 1385, 1420, 1717, 1741
MaeI CTAG 1 cut(s) 456
MaeIII GTNAC 4 cut(s) 812, 1024, 1477, 1822
MalI GATC 6 cut(s) 435, 510, 998, 1210, 1244, 1669
MbiI CCGCTC 3 cut(s) 97, 300, 382
MboI GATC 6 cut(s) 433, 508, 996, 1208, 1242, 1667
MflI RGATCY 1 cut(s) 1242
Mly113I GGCGCC 1 cut(s) 228
MlyI GAGTC 4 cut(s) 179, 454, 1111, 1908
MmeI TCCRAC 3 cut(s) 55, 1201, 1876
MroXI GAANNNNTTC 2 cut(s) 338, 576
MseI TTAA 7 cut(s) 348, 440, 951, 1457, 1491, 1871, 1934
MslI CAYNNNNRTG 1 cut(s) 833
MspA1I CMGCKG 1 cut(s) 1571
MspI CCGG 2 cut(s) 1367, 1960
MspR9I CCNGG 5 cut(s) 23, 1206, 1302, 1496, 1960
Mva1269I GAATGC 1 cut(s) 271
MvaI CCWGG 4 cut(s) 23, 1206, 1302, 1496
MvnI CGCG 3 cut(s) 174, 420, 826
NarI GGCGCC 1 cut(s) 228
NciI CCSGG 1 cut(s) 1960
NdeII GATC 6 cut(s) 433, 508, 996, 1208, 1242, 1667
NlaIII CATG 3 cut(s) 412, 509, 838
NlaIV GGNNCC 4 cut(s) 79, 229, 1465, 2044
NmeAIII GCCGAG 1 cut(s) 286
NmuCI GTSAC 2 cut(s) 812, 1477
NspI RCATGY 1 cut(s) 838
PaqCI CACCTGC 1 cut(s) 643
PauI GCGCGC 1 cut(s) 824
PciSI GCTCTTC 1 cut(s) 329
PctI GAATGC 1 cut(s) 271
PdmI GAANNNNTTC 2 cut(s) 338, 576
PfeI GAWTC 4 cut(s) 13, 450, 1259, 1971
PleI GAGTC 4 cut(s) 179, 453, 1111, 1907
PluTI GGCGCC 1 cut(s) 231
PpsI GAGTC 4 cut(s) 179, 453, 1111, 1907
Psp6I CCWGG 4 cut(s) 21, 1204, 1300, 1494
PspEI GGTNACC 1 cut(s) 812
PspGI CCWGG 4 cut(s) 21, 1204, 1300, 1494
PspN4I GGNNCC 4 cut(s) 79, 229, 1465, 2044
PsrI GAACNNNNNNTAC 2 cut(s) 735, 767
PstI CTGCAG 1 cut(s) 1681
PstNI CAGNNNCTG 1 cut(s) 1987
PsuI RGATCY 1 cut(s) 1242
PteI GCGCGC 1 cut(s) 824
PvuII CAGCTG 1 cut(s) 1571
RsaI GTAC 5 cut(s) 965, 1056, 1185, 1249, 1888
RsaNI GTAC 5 cut(s) 964, 1055, 1184, 1248, 1887
RseI CAYNNNNRTG 1 cut(s) 833
SapI GCTCTTC 1 cut(s) 329
SaqAI TTAA 7 cut(s) 348, 440, 951, 1457, 1491, 1871, 1934
Sau3AI GATC 6 cut(s) 433, 508, 996, 1208, 1242, 1667
ScaI AGTACT 1 cut(s) 1888
SchI GAGTC 4 cut(s) 179, 454, 1111, 1908
ScrFI CCNGG 5 cut(s) 23, 1206, 1302, 1496, 1960
SfaNI GCATC 9 cut(s) 348, 743, 846, 1300, 1367, 1385, 1420, 1717, 1741
SfcI CTRYAG 1 cut(s) 1677
SfoI GGCGCC 1 cut(s) 229
SmiMI CAYNNNNRTG 1 cut(s) 833
SmlI CTYRAG 2 cut(s) 755, 1793
SmoI CTYRAG 2 cut(s) 755, 1793
SspDI GGCGCC 1 cut(s) 227
SspI AATATT 1 cut(s) 1097
SspMI CTAG 1 cut(s) 456
StyD4I CCNGG 5 cut(s) 21, 1204, 1300, 1494, 1958
StyI CCWWGG 2 cut(s) 374, 2038
TaaI ACNGT 5 cut(s) 554, 1011, 1230, 1506, 1923
TaqI TCGA 7 cut(s) 5, 180, 255, 448, 873, 1736, 1902
TaqII GACCGA 1 cut(s) 233
TatI WGTACW 2 cut(s) 1054, 1886
TauI GCSGC 5 cut(s) 97, 159, 162, 303, 423
TfiI GAWTC 4 cut(s) 13, 450, 1259, 1971
Tru1I TTAA 7 cut(s) 348, 440, 951, 1457, 1491, 1871, 1934
Tru9I TTAA 7 cut(s) 348, 440, 951, 1457, 1491, 1871, 1934
TscAI CASTG 4 cut(s) 116, 730, 1863, 1926
TseFI GTSAC 2 cut(s) 812, 1477
TseI GCWGC 9 cut(s) 469, 500, 1277, 1355, 1394, 1424, 1568, 1655, 1730
Tsp45I GTSAC 2 cut(s) 812, 1477
TspDTI ATGAA 4 cut(s) 225, 969, 1222, 1928
TspGWI ACGGA 2 cut(s) 296, 1235
TspRI CASTG 4 cut(s) 116, 730, 1863, 1926
XapI RAATTY 5 cut(s) 477, 487, 576, 1550, 1627
XceI RCATGY 1 cut(s) 838
XmiI GTMKAC 1 cut(s) 1201
XmnI GAANNNNTTC 2 cut(s) 338, 576
XspI CTAG 1 cut(s) 456
ZrmI AGTACT 1 cut(s) 1888
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.