Rh7DG063300

protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0001220
Physical Location & Seq
Reverse (-)
4919655 .. 4931198
11544 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG063300.1

Sequence Viewer

Length: 1626 bp
ATGAAGCAGATGACAGCTCTGAATCACAAAGGAAATGGAATGATAATGGCTATGAAGAAGGCGGTGAGGCCAAAGAAGATACCAAATCGCTCGAGCATGAGAGCAGGTAAGTTTGTTGTGGCATATGCACCTGCTTGTAGTGAAAAAGAGTATTACCTTGCTTCTGCTTGTCAAGAGATTTTATGCCCCTCCAAGTGCTTATTTTTCTTTGTTTGGTCTCAGCTTTCAGGCATCATTTGCATTCTTGAGAAAATTGGAGTTGAGCCACAAGTGGAGAGGATTGGTAAATATAGAAGTGCTGGTGACCAACTTGCGTGCACGACCATGTCCGAAAAAAATTGTGAGATGCTGACAGCATTGCTCGATAACATATATGGGAACTGGCTGGACATAATTTCTTCTACAAGAGGAAAAAAGAGAGAAGATATTGAGAAGTTTGTTAATGAAGGGGTGTACCAAGTAGAAAAGCTGAAAGAAGAGGGCTGGATCACAAACATACAGTATGATGATGAGGTTACTTCAATGTTGAAGGAAAGACTTGGAGTACAAAAAGAGAAAAAGCTTCCTATGGTTGATTACAGGAAATATTCTAAGGTCCGGAAATGGACTCTTGGTTTATCAGGCGGTAAAGACAAGATAGCCATAATAAGAGCCTCAGGCAGTATTAGTCGTGTACGAGGTTCTTTTAGTCTACCCGGATCATCTATTGTTGGAGAACAGTTCATTGAGAAGTTCCGTAGTATAAGAGGTATTGTGAATCAAAAAGATACAAAGGCTGCTATTATCCGAATTGACAGCCCTGGAGGTGATGCCCTTGCTTCTGATTTGATGTGGAGGGAAATCAAACTTTTGGCTGCATCAAAACCGGTCATTGCATCAATGTCTGACGTGGCAGCAAGTGGAGGATACTATATGGCTATGGCAGCAGATGCCATTGTTGCAGAAAGTCTAACCTTAACTGGTTCCATTGGAGTTGTCATAGGGAAGTTTAACCTTGGAAAACTGTATGAGAAGATTGCATTCAACAAAGAAATTATATCACGGGGAAAATTTGCGGAGGTTCTTGCAGCTGAACAACGACCCTTCAGGTGCACTGTCTTGTTGCATACTCTATTTTGTCTGATTTCTTTTATAATCCTGAAAGGAGACAAAATTATTAGTTCTCTTTTTCTTCCTAATTCAGACCAGAGGAAGTTTGCTAAGTCTGCACAGAATTCGTATAAGCAATTTCGGGACAAGGCAGCTTCTTCCAGATCAATGACTGTAGATAAAATGGAGGAAGTTGCACAAGGGAGAGTTTGGGCTGGTAAAGATGCAGCTTCAAGGGGTTTAGTTGATGCTATTGGTGGACTTTCTCGGGCTGTTGCAATAGCAAAGCTCAAGGCAAATATACCACAAGACACAGAGGTTACACTGGTGGAACTTGTAAGACCCTCCCCTTCACTGCCAGAACTTTTAAGTAGCGTAGGGAGTACTCTGGTTGGAGTTGAGCAAACGATGAAGACACTGCTGCAAGACTTAACATTTTCTGATGGGGTTCAAGCCCGGATGGACGGAATCTTGTTTCAGAGACTGGAGGGTTCTTCTAATGCCAACCCCATCTTCAATTTGATGAGAGATTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_S49

Protein Analysis

541

Amino Acids

59.71

Weight (kDa)

9.41

Isoelectric Point (pI)

38.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S49 PF01343 34 - 182 2.9e-13 Peptidase family S49
Peptidase_S49 PF01343 284 - 357 2.1e-21 Peptidase family S49
Peptidase_S49 PF01343 393 - 463 2.9e-12 Peptidase family S49
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1133
AarI CACCTGC 1 cut(s) 139
Acc36I ACCTGC 2 cut(s) 95, 139
AccI GTMKAC 1 cut(s) 691
AccIII TCCGGA 1 cut(s) 597
AciI CCGC 3 cut(s) 62, 624, 1055
AclWI GGATC 2 cut(s) 494, 706
AcsI RAATTY 2 cut(s) 1049, 1213
AcuI CTGAAG 1 cut(s) 1069
AfaI GTAC 4 cut(s) 455, 546, 675, 1474
AgeI ACCGGT 1 cut(s) 865
AgsI TTSAA 6 cut(s) 522, 529, 1024, 1323, 1541, 1606
AjiI CACGTC 1 cut(s) 889
AjnI CCWGG 1 cut(s) 799
AjuI GAANNNNNNNTTGG 2 cut(s) 1586, 1618
AluBI AGCT 8 cut(s) 17, 223, 469, 562, 1070, 1244, 1319, 1378
AluI AGCT 8 cut(s) 17, 223, 469, 562, 1070, 1244, 1319, 1378
Alw21I GWGCWC 2 cut(s) 320, 1094
Alw26I GTCTC 3 cut(s) 222, 1140, 1564
Alw44I GTGCAC 2 cut(s) 316, 1090
AlwI GGATC 2 cut(s) 494, 706
AlwNI CAGNNNCTG 1 cut(s) 1573
Ama87I CYCGRG 2 cut(s) 91, 1356
Aor13HI TCCGGA 1 cut(s) 597
AoxI GGCC 1 cut(s) 68
ApaLI GTGCAC 2 cut(s) 316, 1090
ApeKI GCWGC 8 cut(s) 776, 854, 893, 923, 1067, 1241, 1316, 1510
ApoI RAATTY 2 cut(s) 1049, 1213
AsiGI ACCGGT 1 cut(s) 865
AspS9I GGNCC 1 cut(s) 595
AsuC2I CCSGG 2 cut(s) 696, 1546
AsuHPI GGTGA 3 cut(s) 76, 314, 818
AvaI CYCGRG 2 cut(s) 91, 1356
AvaII GGWCC 1 cut(s) 595
AxyI CCTNAGG 1 cut(s) 655
BaeGI GKGCMC 2 cut(s) 320, 1094
BbsI GAAGAC 1 cut(s) 1508
Bbv12I GWGCWC 2 cut(s) 320, 1094
BbvI GCAGC 8 cut(s) 763, 841, 905, 935, 1079, 1253, 1328, 1497
BccI CCATC 3 cut(s) 1526, 1543, 1607
BcgI CGANNNNNNTGC 2 cut(s) 1197, 1231
BciT130I CCWGG 1 cut(s) 801
BciVI GTATCC 1 cut(s) 899
BcnI CCSGG 2 cut(s) 696, 1546
BcoDI GTCTC 3 cut(s) 222, 1140, 1564
BfmI CTRYAG 1 cut(s) 1263
BfuAI ACCTGC 2 cut(s) 95, 139
BfuI GTATCC 1 cut(s) 899
BisI GCNGC 8 cut(s) 777, 855, 894, 924, 1068, 1242, 1317, 1511
BlsI GCNGC 8 cut(s) 778, 856, 895, 925, 1069, 1243, 1318, 1512
BmcAI AGTACT 1 cut(s) 1474
Bme1390I CCNGG 3 cut(s) 696, 801, 1546
Bme18I GGWCC 1 cut(s) 595
BmeT110I CYCGRG 2 cut(s) 91, 1356
BmgBI CACGTC 1 cut(s) 889
BmgT120I GGNCC 1 cut(s) 595
BmiI GGNNCC 1 cut(s) 964
BmrFI CCNGG 3 cut(s) 696, 801, 1546
BmsI GCATC 8 cut(s) 240, 336, 799, 866, 884, 919, 1303, 1327
BpiI GAAGAC 1 cut(s) 1508
BpmI CTGGAG 2 cut(s) 822, 1595
BpuEI CTTGAG 2 cut(s) 266, 1364
BpuMI CCSGG 2 cut(s) 696, 1546
BsaI GGTCTC 1 cut(s) 222
BsaJI CCNNGG 2 cut(s) 799, 994
BsaWI WCCGGW 2 cut(s) 597, 865
Bse118I RCCGGY 1 cut(s) 865
Bse1I ACTGG 4 cut(s) 386, 964, 1419, 1578
Bse21I CCTNAGG 1 cut(s) 655
Bse3DI GCAATG 2 cut(s) 356, 870
BseAI TCCGGA 1 cut(s) 597
BseBI CCWGG 1 cut(s) 801
BseDI CCNNGG 2 cut(s) 799, 994
BseGI GGATG 1 cut(s) 1554
BseMI GCAATG 2 cut(s) 356, 870
BseMII CTCAG 2 cut(s) 233, 669
BseNI ACTGG 4 cut(s) 386, 964, 1419, 1578
BseSI GKGCMC 2 cut(s) 320, 1094
BseXI GCAGC 8 cut(s) 763, 841, 905, 935, 1079, 1253, 1328, 1497
BsgI GTGCAG 1 cut(s) 1191
BshFI GGCC 1 cut(s) 70
BshTI ACCGGT 1 cut(s) 865
BsiHKAI GWGCWC 2 cut(s) 320, 1094
BsiHKCI CYCGRG 2 cut(s) 91, 1356
BsiSI CCGG 4 cut(s) 598, 696, 866, 1546
BslFI GGGAC 1 cut(s) 1247
BsmAI GTCTC 3 cut(s) 222, 1140, 1564
BsmFI GGGAC 1 cut(s) 1247
BsmI GAATGC 2 cut(s) 240, 1019
BsnI GGCC 1 cut(s) 70
Bso31I GGTCTC 1 cut(s) 222
BsoBI CYCGRG 2 cut(s) 91, 1356
Bsp1286I GDGCHC 2 cut(s) 320, 1094
Bsp13I TCCGGA 1 cut(s) 597
Bsp143I GATC 3 cut(s) 486, 698, 1253
BspACI CCGC 3 cut(s) 62, 624, 1055
BspANI GGCC 1 cut(s) 70
BspCNI CTCAG 2 cut(s) 232, 668
BspEI TCCGGA 1 cut(s) 597
BspLI GGNNCC 1 cut(s) 964
BspMI ACCTGC 2 cut(s) 95, 139
BspPI GGATC 2 cut(s) 494, 706
BspTNI GGTCTC 1 cut(s) 222
BsrDI GCAATG 2 cut(s) 356, 870
BsrFI RCCGGY 1 cut(s) 865
BsrI ACTGG 4 cut(s) 386, 964, 1419, 1578
BssAI RCCGGY 1 cut(s) 865
BssECI CCNNGG 2 cut(s) 799, 994
BssMI GATC 3 cut(s) 486, 698, 1253
BssT1I CCWWGG 1 cut(s) 994
Bst2UI CCWGG 1 cut(s) 801
Bst4CI ACNGT 5 cut(s) 501, 720, 1005, 1096, 1264
Bst6I CTCTTC 1 cut(s) 471
BstAPI GCANNNNNTGC 2 cut(s) 237, 929
BstC8I GCNNGC 1 cut(s) 316
BstDEI CTNAG 4 cut(s) 219, 591, 655, 1200
BstEII GGTNACC 1 cut(s) 302
BstF5I GGATG 1 cut(s) 1554
BstKTI GATC 3 cut(s) 489, 701, 1256
BstMAI GTCTC 3 cut(s) 222, 1140, 1564
BstMBI GATC 3 cut(s) 486, 698, 1253
BstMWI GCNNNNNNNGC 5 cut(s) 237, 923, 929, 938, 1205
BstNI CCWGG 1 cut(s) 801
BstPI GGTNACC 1 cut(s) 302
BstSCI CCNGG 3 cut(s) 694, 799, 1544
BstSFI CTRYAG 1 cut(s) 1263
BstSLI GKGCMC 2 cut(s) 320, 1094
BstV1I GCAGC 8 cut(s) 763, 841, 905, 935, 1079, 1253, 1328, 1497
BstV2I GAAGAC 1 cut(s) 1508
Bsu36I CCTNAGG 1 cut(s) 655
BsuI GTATCC 1 cut(s) 899
BsuRI GGCC 1 cut(s) 70
BtrI CACGTC 1 cut(s) 889
BtsCI GGATG 1 cut(s) 1554
BtsI GCAGTG 2 cut(s) 1442, 1505
BtsIMutI CAGTG 4 cut(s) 1092, 1412, 1442, 1505
BveI ACCTGC 2 cut(s) 95, 139
Cac8I GCNNGC 1 cut(s) 316
CaiI CAGNNNCTG 1 cut(s) 1573
Cfr10I RCCGGY 1 cut(s) 865
Cfr13I GGNCC 1 cut(s) 595
Csp6I GTAC 4 cut(s) 454, 545, 674, 1473
CspAI ACCGGT 1 cut(s) 865
CviAII CATG 2 cut(s) 97, 325
CviQI GTAC 4 cut(s) 454, 545, 674, 1473
DdeI CTNAG 4 cut(s) 219, 591, 655, 1200
DpnI GATC 3 cut(s) 488, 700, 1255
DpnII GATC 3 cut(s) 486, 698, 1253
Eam1104I CTCTTC 1 cut(s) 471
EarI CTCTTC 1 cut(s) 471
Eco130I CCWWGG 1 cut(s) 994
Eco31I GGTCTC 1 cut(s) 222
Eco47I GGWCC 1 cut(s) 595
Eco57I CTGAAG 1 cut(s) 1069
Eco81I CCTNAGG 1 cut(s) 655
Eco88I CYCGRG 2 cut(s) 91, 1356
Eco91I GGTNACC 1 cut(s) 302
EcoO65I GGTNACC 1 cut(s) 302
EcoRI GAATTC 1 cut(s) 1213
EcoRII CCWGG 1 cut(s) 799
EcoT14I CCWWGG 1 cut(s) 994
ErhI CCWWGG 1 cut(s) 994
FaeI CATG 2 cut(s) 100, 328
FaqI GGGAC 1 cut(s) 1247
FatI CATG 2 cut(s) 96, 324
FauNDI CATATG 1 cut(s) 124
FblI GTMKAC 1 cut(s) 691
Fnu4HI GCNGC 8 cut(s) 777, 855, 894, 924, 1068, 1242, 1317, 1511
FokI GGATG 1 cut(s) 1561
Fsp4HI GCNGC 8 cut(s) 777, 855, 894, 924, 1068, 1242, 1317, 1511
GluI GCNGC 8 cut(s) 777, 855, 894, 924, 1068, 1242, 1317, 1511
GsuI CTGGAG 2 cut(s) 822, 1595
HaeIII GGCC 1 cut(s) 70
HapII CCGG 4 cut(s) 598, 696, 866, 1546
Hin1II CATG 2 cut(s) 100, 328
HindIII AAGCTT 1 cut(s) 560
HinfI GANTC 4 cut(s) 22, 607, 757, 1557
HpaII CCGG 4 cut(s) 598, 696, 866, 1546
HphI GGTGA 3 cut(s) 76, 314, 818
Hpy166II GTNNAC 6 cut(s) 318, 454, 674, 692, 1092, 1349
Hpy188I TCNGA 9 cut(s) 21, 331, 788, 823, 886, 1122, 1183, 1531, 1569
Hpy188III TCNNGA 6 cut(s) 173, 245, 598, 1138, 1232, 1251
Hpy8I GTNNAC 6 cut(s) 318, 454, 674, 692, 1092, 1349
HpyAV CCTTC 5 cut(s) 52, 440, 523, 1093, 1449
HpyCH4III ACNGT 5 cut(s) 501, 720, 1005, 1096, 1264
HpyCH4IV ACGT 1 cut(s) 888
HpyF10VI GCNNNNNNNGC 5 cut(s) 237, 923, 929, 938, 1205
HpyF3I CTNAG 4 cut(s) 219, 591, 655, 1200
HpySE526I ACGT 1 cut(s) 888
Hsp92II CATG 2 cut(s) 100, 328
Kpn2I TCCGGA 1 cut(s) 597
Kzo9I GATC 3 cut(s) 486, 698, 1253
Lsp1109I GCAGC 8 cut(s) 763, 841, 905, 935, 1079, 1253, 1328, 1497
LweI GCATC 8 cut(s) 240, 336, 799, 866, 884, 919, 1303, 1327
MaeII ACGT 1 cut(s) 888
MaeIII GTNAC 3 cut(s) 302, 514, 1408
MalI GATC 3 cut(s) 488, 700, 1255
MboI GATC 3 cut(s) 486, 698, 1253
MhlI GDGCHC 2 cut(s) 320, 1094
MlyI GAGTC 1 cut(s) 601
MmeI TCCRAC 2 cut(s) 691, 1462
MroI TCCGGA 1 cut(s) 597
MseI TTAA 5 cut(s) 441, 956, 990, 1457, 1520
MslI CAYNNNNRTG 1 cut(s) 323
MspA1I CMGCKG 1 cut(s) 1070
MspI CCGG 4 cut(s) 598, 696, 866, 1546
MspR9I CCNGG 3 cut(s) 696, 801, 1546
Mva1269I GAATGC 2 cut(s) 240, 1019
MvaI CCWGG 1 cut(s) 801
MwoI GCNNNNNNNGC 5 cut(s) 237, 923, 929, 938, 1205
NciI CCSGG 2 cut(s) 696, 1546
NdeI CATATG 1 cut(s) 124
NdeII GATC 3 cut(s) 486, 698, 1253
NlaIII CATG 2 cut(s) 100, 328
NlaIV GGNNCC 1 cut(s) 964
NmuCI GTSAC 1 cut(s) 302
PaeR7I CTCGAG 1 cut(s) 91
PaqCI CACCTGC 1 cut(s) 139
PctI GAATGC 2 cut(s) 240, 1019
PfeI GAWTC 3 cut(s) 22, 757, 1557
PflFI GACNNNGTC 1 cut(s) 325
PinAI ACCGGT 1 cut(s) 865
PkrI GCNGC 8 cut(s) 778, 856, 895, 925, 1069, 1243, 1318, 1512
PleI GAGTC 1 cut(s) 601
PpsI GAGTC 1 cut(s) 601
PsiI TTATAA 1 cut(s) 1133
Psp6I CCWGG 1 cut(s) 799
PspEI GGTNACC 1 cut(s) 302
PspGI CCWGG 1 cut(s) 799
PspN4I GGNNCC 1 cut(s) 964
PspPI GGNCC 1 cut(s) 595
PspXI VCTCGAGB 1 cut(s) 91
PstNI CAGNNNCTG 1 cut(s) 1573
PsyI GACNNNGTC 1 cut(s) 325
PvuII CAGCTG 1 cut(s) 1070
RsaI GTAC 4 cut(s) 455, 546, 675, 1474
RsaNI GTAC 4 cut(s) 454, 545, 674, 1473
RseI CAYNNNNRTG 1 cut(s) 323
SaqAI TTAA 5 cut(s) 441, 956, 990, 1457, 1520
SatI GCNGC 8 cut(s) 777, 855, 894, 924, 1068, 1242, 1317, 1511
Sau3AI GATC 3 cut(s) 486, 698, 1253
Sau96I GGNCC 1 cut(s) 595
ScaI AGTACT 1 cut(s) 1474
SchI GAGTC 1 cut(s) 601
ScrFI CCNGG 3 cut(s) 696, 801, 1546
SduI GDGCHC 2 cut(s) 320, 1094
SfaNI GCATC 8 cut(s) 240, 336, 799, 866, 884, 919, 1303, 1327
SfcI CTRYAG 1 cut(s) 1263
Sfr274I CTCGAG 1 cut(s) 91
SinI GGWCC 1 cut(s) 595
SlaI CTCGAG 1 cut(s) 91
SmiMI CAYNNNNRTG 1 cut(s) 323
SmlI CTYRAG 3 cut(s) 91, 245, 1379
SmoI CTYRAG 3 cut(s) 91, 245, 1379
SsiI CCGC 3 cut(s) 62, 624, 1055
SspI AATATT 1 cut(s) 587
StyD4I CCNGG 3 cut(s) 694, 799, 1544
StyI CCWWGG 1 cut(s) 994
TaaI ACNGT 5 cut(s) 501, 720, 1005, 1096, 1264
TaiI ACGT 1 cut(s) 891
TaqI TCGA 2 cut(s) 92, 363
TatI WGTACW 2 cut(s) 544, 1472
TfiI GAWTC 3 cut(s) 22, 757, 1557
Tru1I TTAA 5 cut(s) 441, 956, 990, 1457, 1520
Tru9I TTAA 5 cut(s) 441, 956, 990, 1457, 1520
TscAI CASTG 4 cut(s) 1099, 1419, 1449, 1512
TseFI GTSAC 1 cut(s) 302
TseI GCWGC 8 cut(s) 776, 854, 893, 923, 1067, 1241, 1316, 1510
Tsp45I GTSAC 1 cut(s) 302
TspDTI ATGAA 5 cut(s) 17, 68, 459, 712, 1514
TspGWI ACGGA 2 cut(s) 725, 1569
TspRI CASTG 4 cut(s) 1099, 1419, 1449, 1512
Tth111I GACNNNGTC 1 cut(s) 325
VneI GTGCAC 2 cut(s) 316, 1090
VpaK11BI GGWCC 1 cut(s) 595
XapI RAATTY 2 cut(s) 1049, 1213
XhoI CTCGAG 1 cut(s) 91
XmiI GTMKAC 1 cut(s) 691
ZrmI AGTACT 1 cut(s) 1474
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.