Rh7BG469900

protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7B
OG0001220
Physical Location & Seq
Reverse (-)
55508300 .. 55525572
17273 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7BG469900.1

Sequence Viewer

Length: 1236 bp
ATGCGGAGAATGCCAATGCTACGACGGACAAGGACTATCCGAGCGGCGAGTCTCTGGAAGAGCTTCGTCGTCAAGAAGGGAAGCGTCTTCACCGTGACATTGCGTGGCCAGATAACCGATCAGTTAAAGAGTCGAATTGCTGGTGTGTATCTTCAAATTGAGCGCTTGAACTGTGGATGGGGTAAAGTGGAAGAAATTCGGAGGCATATTTGGACTTCCAGAAATCAGGCATCATTTGTAAGAGGTGTTCTTGAGAAAATTGGAGTTGAGCCACAAGTGGAAAGGATTGGTGAATATAAAAGTGCTGGTGACCAACTTGCGCGCACGACCATGTCCGAAGAAAATTGTGAGATGCTGACAGCATTGCTCGATAACACATATGGGAACTGGCTGGACATAATTTCTTCTACAAGAGGAAAAAAGAGAGAAGATATTGAGAAGTTTGTTAATGAAGGGGTGTACCAAGTAGAAAAGCTGAAAGAAGAGGGCTGGATCACAAACATACAGTATGATGATGAGGTTACTTCAATGTTGAAGGAAAGACTTGGAGTACAAAAAGAGAAAAAGCTTCCTATGGTTGATTACAGGAAATATTCTAAGGTCCGGAAATGGACTCTTGGTTTATCAGGCGGTAAAGACAAGATAGCCATAATAAGAGCCTCAGGCAGTATTAGTCTTGTACGAAGTTCTTTTAGTCTACCTGGATCATCTATTGTTGGAGATCAGTTCATTGAGAAGATCCGTACTATAAGAGAGTCAAAAAGATACAAGGCTGCTATTATCCGAATTGACAGCCCTGGAGGTGATGCCCTTGCTTCTTATTTGATGTGGAGGGAAATCAAACTTTTGGCTGCATCAAAACCGTTGTCACAGGGAAGTTTAACCTTGGAAAACTGTGAGAAGATTGGCTTCAACAAAGAAATCATATCACGGGGAAAATTTGCAGAGGTTCTTGCAGCTGAACAACGACCATTCAGACCAGAGGAAGCTGAACTTTTTGCTAAGTCTGCACAGAATTCGTATAAGCAATTTCGGAGCAAGGCAGCTTCTTCCAGATCAATGACTGTAGATAAAATGGAGGAAGTTGCACAAGGGAGAGTTTGGGCTGGTAAAGATGCAGCTTCAAGGGGTTTAGTTGATGCAGATTCCTCAAGATTTGGTTCCTCTATTGGTGGACTTTGTGACAAAAATCTTATTTCATTGGCTAGGCATATACCAGAGATATTAATAAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_S49

Protein Analysis

411

Amino Acids

46.36

Weight (kDa)

9.76

Isoelectric Point (pI)

42.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S49 PF01343 76 - 184 1e-13 Peptidase family S49
Peptidase_S49 PF01343 299 - 381 1.2e-14 Peptidase family S49
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 44
AccI GTMKAC 1 cut(s) 697
AccII CGCG 1 cut(s) 322
AccIII TCCGGA 1 cut(s) 603
AciI CCGC 3 cut(s) 4, 44, 630
AclWI GGATC 3 cut(s) 500, 712, 733
AcoI YGGCCR 1 cut(s) 106
AcsI RAATTY 3 cut(s) 195, 938, 1015
AfaI GTAC 4 cut(s) 461, 552, 681, 745
AfeI AGCGCT 1 cut(s) 164
AgsI TTSAA 6 cut(s) 155, 169, 528, 535, 913, 1125
AjnI CCWGG 2 cut(s) 700, 796
AluBI AGCT 7 cut(s) 63, 475, 568, 959, 989, 1046, 1121
AluI AGCT 7 cut(s) 63, 475, 568, 959, 989, 1046, 1121
Alw26I GTCTC 1 cut(s) 56
AlwI GGATC 3 cut(s) 500, 712, 733
Aor13HI TCCGGA 1 cut(s) 603
Aor51HI AGCGCT 1 cut(s) 164
AoxI GGCC 1 cut(s) 106
ApeKI GCWGC 5 cut(s) 773, 851, 956, 1043, 1118
ApoI RAATTY 3 cut(s) 195, 938, 1015
ArsI GACNNNNNNTTYG 2 cut(s) 851, 883
AseI ATTAAT 1 cut(s) 1226
Asp700I GAANNNNTTC 2 cut(s) 62, 195
AspLEI GCGC 3 cut(s) 165, 322, 324
AspS9I GGNCC 1 cut(s) 601
AsuHPI GGTGA 4 cut(s) 82, 302, 320, 815
AvaII GGWCC 1 cut(s) 601
AxyI CCTNAGG 1 cut(s) 661
BalI TGGCCA 1 cut(s) 108
BbsI GAAGAC 1 cut(s) 79
BbvI GCAGC 5 cut(s) 760, 838, 968, 1055, 1130
BccI CCATC 1 cut(s) 171
BcgI CGANNNNNNTGC 2 cut(s) 999, 1033
BciT130I CCWGG 2 cut(s) 702, 798
BcoDI GTCTC 1 cut(s) 56
BfaI CTAG 1 cut(s) 1206
BfmI CTRYAG 1 cut(s) 1065
BfoI RGCGCY 1 cut(s) 166
BisI GCNGC 6 cut(s) 45, 774, 852, 957, 1044, 1119
BlsI GCNGC 6 cut(s) 46, 775, 853, 958, 1045, 1120
Bme1390I CCNGG 2 cut(s) 702, 798
Bme18I GGWCC 1 cut(s) 601
BmgT120I GGNCC 1 cut(s) 601
BmiI GGNNCC 1 cut(s) 1162
BmrFI CCNGG 2 cut(s) 702, 798
BmsI GCATC 6 cut(s) 239, 342, 796, 863, 1105, 1129
BpiI GAAGAC 1 cut(s) 79
BpmI CTGGAG 1 cut(s) 819
BpuEI CTTGAG 2 cut(s) 272, 1135
BsaJI CCNNGG 2 cut(s) 796, 885
BsaWI WCCGGW 1 cut(s) 603
Bse1I ACTGG 1 cut(s) 392
Bse21I CCTNAGG 1 cut(s) 661
Bse3DI GCAATG 2 cut(s) 98, 362
BseAI TCCGGA 1 cut(s) 603
BseBI CCWGG 2 cut(s) 702, 798
BseDI CCNNGG 2 cut(s) 796, 885
BseGI GGATG 1 cut(s) 182
BseMI GCAATG 2 cut(s) 98, 362
BseMII CTCAG 1 cut(s) 675
BseNI ACTGG 1 cut(s) 392
BsePI GCGCGC 1 cut(s) 320
BseXI GCAGC 5 cut(s) 760, 838, 968, 1055, 1130
BsgI GTGCAG 1 cut(s) 993
Bsh1236I CGCG 1 cut(s) 322
BshFI GGCC 1 cut(s) 108
BsiSI CCGG 1 cut(s) 604
BsmAI GTCTC 1 cut(s) 56
BsmI GAATGC 1 cut(s) 15
BsnI GGCC 1 cut(s) 108
Bsp13I TCCGGA 1 cut(s) 603
Bsp143I GATC 6 cut(s) 118, 492, 704, 721, 738, 1055
BspACI CCGC 3 cut(s) 4, 44, 630
BspANI GGCC 1 cut(s) 108
BspCNI CTCAG 1 cut(s) 674
BspEI TCCGGA 1 cut(s) 603
BspFNI CGCG 1 cut(s) 322
BspLI GGNNCC 1 cut(s) 1162
BspPI GGATC 3 cut(s) 500, 712, 733
BspQI GCTCTTC 1 cut(s) 53
BsrBI CCGCTC 1 cut(s) 44
BsrDI GCAATG 2 cut(s) 98, 362
BsrI ACTGG 1 cut(s) 392
BssECI CCNNGG 2 cut(s) 796, 885
BssHII GCGCGC 1 cut(s) 320
BssMI GATC 6 cut(s) 118, 492, 704, 721, 738, 1055
BssT1I CCWWGG 1 cut(s) 885
Bst2UI CCWGG 2 cut(s) 702, 798
Bst4CI ACNGT 6 cut(s) 94, 173, 507, 864, 896, 1066
Bst6I CTCTTC 2 cut(s) 53, 477
BstC8I GCNNGC 1 cut(s) 322
BstDEI CTNAG 3 cut(s) 597, 661, 1002
BstEII GGTNACC 1 cut(s) 308
BstF5I GGATG 1 cut(s) 182
BstFNI CGCG 1 cut(s) 322
BstH2I RGCGCY 1 cut(s) 166
BstHHI GCGC 3 cut(s) 165, 322, 324
BstKTI GATC 6 cut(s) 121, 495, 707, 724, 741, 1058
BstMAI GTCTC 1 cut(s) 56
BstMBI GATC 6 cut(s) 118, 492, 704, 721, 738, 1055
BstMWI GCNNNNNNNGC 2 cut(s) 10, 1007
BstNI CCWGG 2 cut(s) 702, 798
BstPI GGTNACC 1 cut(s) 308
BstSCI CCNGG 2 cut(s) 700, 796
BstSFI CTRYAG 1 cut(s) 1065
BstUI CGCG 1 cut(s) 322
BstV1I GCAGC 5 cut(s) 760, 838, 968, 1055, 1130
BstV2I GAAGAC 1 cut(s) 79
BstX2I RGATCY 1 cut(s) 738
BstYI RGATCY 1 cut(s) 738
Bsu36I CCTNAGG 1 cut(s) 661
BsuRI GGCC 1 cut(s) 108
BtsCI GGATG 1 cut(s) 182
Cac8I GCNNGC 1 cut(s) 322
CfoI GCGC 3 cut(s) 165, 322, 324
Cfr13I GGNCC 1 cut(s) 601
CseI GACGC 1 cut(s) 73
Csp6I GTAC 4 cut(s) 460, 551, 680, 744
CviAII CATG 1 cut(s) 331
CviQI GTAC 4 cut(s) 460, 551, 680, 744
DdeI CTNAG 3 cut(s) 597, 661, 1002
DpnI GATC 6 cut(s) 120, 494, 706, 723, 740, 1057
DpnII GATC 6 cut(s) 118, 492, 704, 721, 738, 1055
EaeI YGGCCR 1 cut(s) 106
Eam1104I CTCTTC 2 cut(s) 53, 477
EarI CTCTTC 2 cut(s) 53, 477
Eco130I CCWWGG 1 cut(s) 885
Eco47I GGWCC 1 cut(s) 601
Eco47III AGCGCT 1 cut(s) 164
Eco81I CCTNAGG 1 cut(s) 661
Eco91I GGTNACC 1 cut(s) 308
EcoO65I GGTNACC 1 cut(s) 308
EcoRI GAATTC 1 cut(s) 1015
EcoRII CCWGG 2 cut(s) 700, 796
EcoT14I CCWWGG 1 cut(s) 885
ErhI CCWWGG 1 cut(s) 885
FaeI CATG 1 cut(s) 334
FalI AAGNNNNNCTT 4 cut(s) 893, 925, 978, 1010
FatI CATG 1 cut(s) 330
FauNDI CATATG 1 cut(s) 379
FblI GTMKAC 1 cut(s) 697
Fnu4HI GCNGC 6 cut(s) 45, 774, 852, 957, 1044, 1119
FokI GGATG 1 cut(s) 189
Fsp4HI GCNGC 6 cut(s) 45, 774, 852, 957, 1044, 1119
FspBI CTAG 1 cut(s) 1206
GlaI GCGC 3 cut(s) 164, 321, 323
GluI GCNGC 6 cut(s) 45, 774, 852, 957, 1044, 1119
GsuI CTGGAG 1 cut(s) 819
HaeII RGCGCY 1 cut(s) 166
HaeIII GGCC 1 cut(s) 108
HapII CCGG 1 cut(s) 604
HgaI GACGC 1 cut(s) 73
HhaI GCGC 3 cut(s) 165, 322, 324
Hin1II CATG 1 cut(s) 334
Hin6I GCGC 3 cut(s) 163, 320, 322
HinP1I GCGC 3 cut(s) 163, 320, 322
HindIII AAGCTT 1 cut(s) 566
HinfI GANTC 5 cut(s) 49, 130, 613, 755, 1145
HpaII CCGG 1 cut(s) 604
HphI GGTGA 4 cut(s) 82, 302, 320, 815
Hpy166II GTNNAC 3 cut(s) 460, 698, 1175
Hpy188I TCNGA 6 cut(s) 41, 201, 337, 785, 977, 1035
Hpy188III TCNNGA 7 cut(s) 55, 73, 219, 251, 604, 1053, 1152
Hpy8I GTNNAC 3 cut(s) 460, 698, 1175
Hpy99I CGWCG 2 cut(s) 27, 71
HpyAV CCTTC 3 cut(s) 70, 446, 529
HpyCH4III ACNGT 6 cut(s) 94, 173, 507, 864, 896, 1066
HpyCH4V TGCA 7 cut(s) 854, 944, 956, 1010, 1088, 1118, 1142
HpyF10VI GCNNNNNNNGC 2 cut(s) 10, 1007
HpyF3I CTNAG 3 cut(s) 597, 661, 1002
Hsp92II CATG 1 cut(s) 334
HspAI GCGC 3 cut(s) 163, 320, 322
Kpn2I TCCGGA 1 cut(s) 603
Kzo9I GATC 6 cut(s) 118, 492, 704, 721, 738, 1055
LguI GCTCTTC 1 cut(s) 53
LmnI GCTCC 1 cut(s) 1035
Lsp1109I GCAGC 5 cut(s) 760, 838, 968, 1055, 1130
LweI GCATC 6 cut(s) 239, 342, 796, 863, 1105, 1129
MaeI CTAG 1 cut(s) 1206
MaeIII GTNAC 5 cut(s) 94, 308, 520, 867, 1181
MalI GATC 6 cut(s) 120, 494, 706, 723, 740, 1057
MbiI CCGCTC 1 cut(s) 44
MboI GATC 6 cut(s) 118, 492, 704, 721, 738, 1055
MflI RGATCY 1 cut(s) 738
MlsI TGGCCA 1 cut(s) 108
MluNI TGGCCA 1 cut(s) 108
MlyI GAGTC 4 cut(s) 58, 139, 607, 764
MmeI TCCRAC 1 cut(s) 697
Mox20I TGGCCA 1 cut(s) 108
MroI TCCGGA 1 cut(s) 603
MroXI GAANNNNTTC 2 cut(s) 62, 195
MscI TGGCCA 1 cut(s) 108
MseI TTAA 5 cut(s) 125, 447, 881, 1226, 1234
MslI CAYNNNNRTG 1 cut(s) 329
Msp20I TGGCCA 1 cut(s) 108
MspA1I CMGCKG 1 cut(s) 959
MspI CCGG 1 cut(s) 604
MspR9I CCNGG 2 cut(s) 702, 798
Mva1269I GAATGC 1 cut(s) 15
MvaI CCWGG 2 cut(s) 702, 798
MvnI CGCG 1 cut(s) 322
MwoI GCNNNNNNNGC 2 cut(s) 10, 1007
NdeI CATATG 1 cut(s) 379
NdeII GATC 6 cut(s) 118, 492, 704, 721, 738, 1055
NlaIII CATG 1 cut(s) 334
NlaIV GGNNCC 1 cut(s) 1162
NmuCI GTSAC 4 cut(s) 94, 308, 867, 1181
PauI GCGCGC 1 cut(s) 320
PciSI GCTCTTC 1 cut(s) 53
PctI GAATGC 1 cut(s) 15
PdmI GAANNNNTTC 2 cut(s) 62, 195
PfeI GAWTC 1 cut(s) 1145
PflFI GACNNNGTC 1 cut(s) 331
PkrI GCNGC 6 cut(s) 46, 775, 853, 958, 1045, 1120
PleI GAGTC 4 cut(s) 57, 138, 607, 763
PpsI GAGTC 4 cut(s) 57, 138, 607, 763
PshBI ATTAAT 1 cut(s) 1226
Psp6I CCWGG 2 cut(s) 700, 796
PspEI GGTNACC 1 cut(s) 308
PspGI CCWGG 2 cut(s) 700, 796
PspN4I GGNNCC 1 cut(s) 1162
PspPI GGNCC 1 cut(s) 601
PsrI GAACNNNNNNTAC 2 cut(s) 231, 263
PsuI RGATCY 1 cut(s) 738
PsyI GACNNNGTC 1 cut(s) 331
PteI GCGCGC 1 cut(s) 320
PvuII CAGCTG 1 cut(s) 959
RsaI GTAC 4 cut(s) 461, 552, 681, 745
RsaNI GTAC 4 cut(s) 460, 551, 680, 744
RseI CAYNNNNRTG 1 cut(s) 329
SapI GCTCTTC 1 cut(s) 53
SaqAI TTAA 5 cut(s) 125, 447, 881, 1226, 1234
SatI GCNGC 6 cut(s) 45, 774, 852, 957, 1044, 1119
Sau3AI GATC 6 cut(s) 118, 492, 704, 721, 738, 1055
Sau96I GGNCC 1 cut(s) 601
SchI GAGTC 4 cut(s) 58, 139, 607, 764
ScrFI CCNGG 2 cut(s) 702, 798
SfaNI GCATC 6 cut(s) 239, 342, 796, 863, 1105, 1129
SfcI CTRYAG 1 cut(s) 1065
SinI GGWCC 1 cut(s) 601
SmiMI CAYNNNNRTG 1 cut(s) 329
SmlI CTYRAG 2 cut(s) 251, 1150
SmoI CTYRAG 2 cut(s) 251, 1150
SsiI CCGC 3 cut(s) 4, 44, 630
SspI AATATT 1 cut(s) 593
SspMI CTAG 1 cut(s) 1206
StyD4I CCNGG 2 cut(s) 700, 796
StyI CCWWGG 1 cut(s) 885
TaaI ACNGT 6 cut(s) 94, 173, 507, 864, 896, 1066
TaqI TCGA 2 cut(s) 133, 369
TatI WGTACW 1 cut(s) 550
TauI GCSGC 1 cut(s) 47
TfiI GAWTC 1 cut(s) 1145
Tru1I TTAA 5 cut(s) 125, 447, 881, 1226, 1234
Tru9I TTAA 5 cut(s) 125, 447, 881, 1226, 1234
TseFI GTSAC 4 cut(s) 94, 308, 867, 1181
TseI GCWGC 5 cut(s) 773, 851, 956, 1043, 1118
Tsp45I GTSAC 4 cut(s) 94, 308, 867, 1181
TspDTI ATGAA 3 cut(s) 465, 718, 1188
TspGWI ACGGA 2 cut(s) 40, 731
Tth111I GACNNNGTC 1 cut(s) 331
VpaK11BI GGWCC 1 cut(s) 601
VspI ATTAAT 1 cut(s) 1226
XapI RAATTY 3 cut(s) 195, 938, 1015
XmiI GTMKAC 1 cut(s) 697
XmnI GAANNNNTTC 2 cut(s) 62, 195
XspI CTAG 1 cut(s) 1206
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.