MD14G1217600.v1.1

protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0001220
Physical Location & Seq
Reverse (-)
30104138 .. 30108881
4744 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1217600.v1.1.491

Sequence Viewer

Length: 2040 bp
ATGTCGAAGCTCATTCTTCTCCACACTTCTCCGATCCACCGCCGCGCCTTCAATGCCTTCGTTTCGACCTCTCTGTCCAAACCTCTCTCCACTCCCACCTCCCTCCGCTTCCTATGCCTCCGCCCCCAATTCATCTCTCCCTCTCCCCCTCTTTCTCTGCGCAGCATCTCAGCTCGCGCCTTCGACTCGTCTCCGTTGTCGTCGACGGATACCAAAGAAGACGAATCGAAGGAGAGCGATGGCGAGATCGTAAATAAATCCAAAACAGCAGACAAGGAGTATCCGAGCGGCGAGTTTCGGTTCGAGAAGGCGAGCGCGTGGAAGAGCTTCGTCGTCAAGCTCCGCATGCTCATTGCCTTCCCTTGGCAGCGCGTCAAGAAAGGCAGCGTCTTGACCATCAAATTGCGTGGCCAGGTGTCTGATCAGCTGAAGAGTCGATTTTCGAGTGGACTTTCGCTGCCTCAAATTTGTGAGAATTTGATTAAAGCAGCTTACGATCCTCGAATTTCCGGTGTCTATCTCCAAATTGAAAGCTTGAATTGTGGTTGGGGCAAAGTCGAAGAAATCCGAAGACATATCTTGGATTTCAAGAAATCAGGTAAATTTGTTGTGGCCTATGTACCGGCTTGTGGAGAGAAAGAGTATTACCTTGCTAGTGCGTGCCAAGAGATATATGCACCTCCAAGTGCTTATTTTTCTTTGTTTGGTCTCACCGTTCAAGCCTCATTCATAAGAGGTGTTCTTGAGAAAGTTGGAATCGAGCCACAAGTGGAAAGGATTGGTAAATACAAAAGTGCCGGTGATCAACTTGCACGCAAAACCATGTCCAAAGAGAATTGTGAGATGCTGACAGCATTGCTTGATAATATATATGGGAATTGGCTGGATGTGATTTCTTCCACGAGAGGAAAGAAAAGAGAAGATATTGAGAATTTTATTAATGAAGGAGTCTACCAAGTAGAAAAATTGAAAGAAGAAGGCTGGATCACAAACATACAATATGATGACGAGGTTATTTCAATGTTGAAGGAAAGACTTGGAGTACAAAAAGAAAAAACTCTTCCTATGGTTGATTACAAAAAGTACTCAAGGGTTCGGCAATGGACTGTTGGATTATCAGGTGGTAAAGACAAGATAGCCATAATTAGAGCATCAGGCAGCATTACTCGTGTACGAGGCGGTTTAAGTCTTCCCGGTTCTAGTATTATTGGGGAACAGTTCATTGAGAAGATTCGCAGTGTGAGAGAATCAAAAAGATATAAGGCCGCAATCATCCGAATTGACAGCCCTGGAGGTGATGCTCTTGCTTCTGATTTGATGTGGAGGGAAATCAGACTTTTGGCTGCATCAAAACCTGTCATTGCATCGATGTCTGATGTGGCAGCAAGTGGAGGATACTATATGGCAATGGCAGCAGACACCATTGTTGCAGAAAATCTAACCTTAACTGGTTCCATTGGAGTTGTGACAGGGAAGTTTAACCTGGGAAAATTGTATGAGAAGATTGGCTTCAACAAAGAAATCATATCAAGGGGAAAATATGCTGAAGTTCTTGCAGCTGAACAACGACCCTTCAGACCAGAAGAAGCTGAACTGTTTGCTAAGTCTGCACAGAATTCATATAAGCAATTCCGAGACAAGGCGGCTTTTTCCAGATCGATGACTGTAGATAAAATGGAGGAGGTTGCACAAGGGAGAGTTTGGACTGGTAACGATGCAGCTTCACGAGGTTTAGTTGATGCTATTGGCGGATTTTCTCGTGCTGTCGCAATAGCAAAGCTCAAGGCAAATATACCCCAAGACAGACAGGTTGCGCTCGTGGAGCTCGCAAGACCTTCCCCAACGCTGCCAGAACTTTTAAGTGGCATAGGGGCTACATTGGTTGGAGTGGATAGAACAATGAAGGAAGTGCTGCAGGAGTTGACGTTTTCCGACGGAGTCCAAGCCCGAATGGACGGAATCATGTTTCAGAGACTGGAGGGAGCTGCTCAGGCCAACCCCATCTTCTCTTTGTTAAAAGATTACTTTGGTTCCCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_S49

Protein Analysis

680

Amino Acids

75.24

Weight (kDa)

9.39

Isoelectric Point (pI)

42.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S49 PF01343 196 - 348 4.6e-23 Peptidase family S49
Peptidase_S49 PF01343 447 - 597 6.6e-43 Peptidase family S49
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 73
Acc16I TGCGCA 1 cut(s) 161
AccBSI CCGCTC 1 cut(s) 288
AccI GTMKAC 2 cut(s) 203, 951
AccII CGCG 4 cut(s) 45, 177, 317, 372
AclWI GGATC 3 cut(s) 28, 491, 992
AcoI YGGCCR 1 cut(s) 409
AcsI RAATTY 6 cut(s) 465, 475, 504, 602, 931, 1615
AcuI CTGAAG 3 cut(s) 449, 1558, 1566
AfaI GTAC 4 cut(s) 621, 1044, 1085, 1173
AfiI CCNNNNNNNGG 3 cut(s) 363, 629, 1639
AgsI TTSAA 9 cut(s) 52, 530, 538, 589, 719, 970, 1020, 1027, 1513
AjnI CCWGG 3 cut(s) 411, 1288, 1482
AjuI GAANNNNNNNTTGG 2 cut(s) 1988, 2020
Alw21I GWGCWC 1 cut(s) 1827
Alw26I GTCTC 4 cut(s) 195, 713, 1629, 1966
AlwI GGATC 3 cut(s) 28, 491, 992
AlwNI CAGNNNCTG 1 cut(s) 1975
AoxI GGCC 4 cut(s) 409, 612, 1263, 1992
ApoI RAATTY 6 cut(s) 465, 475, 504, 602, 931, 1615
AseI ATTAAT 1 cut(s) 939
Asp700I GAANNNNTTC 1 cut(s) 326
AspLEI GCGC 6 cut(s) 47, 162, 179, 317, 372, 1816
AsuC2I CCSGG 1 cut(s) 1194
AsuHPI GGTGA 3 cut(s) 703, 812, 1307
BalI TGGCCA 1 cut(s) 411
BanII GRGCYC 1 cut(s) 1827
BauI CACGAG 5 cut(s) 901, 1167, 1725, 1758, 1817
BbsI GAAGAC 3 cut(s) 225, 577, 1181
Bbv12I GWGCWC 1 cut(s) 1827
BccI CCATC 3 cut(s) 233, 404, 2009
BciT130I CCWGG 3 cut(s) 413, 1290, 1484
BciVI GTATCC 3 cut(s) 202, 291, 1388
BclI TGATCA 2 cut(s) 421, 802
BcnI CCSGG 1 cut(s) 1194
BcoDI GTCTC 4 cut(s) 195, 713, 1629, 1966
BfaI CTAG 2 cut(s) 654, 1200
BfmI CTRYAG 2 cut(s) 1665, 1913
BfuI GTATCC 3 cut(s) 202, 291, 1388
BmcAI AGTACT 1 cut(s) 1085
Bme1390I CCNGG 4 cut(s) 413, 1194, 1290, 1484
BmiI GGNNCC 2 cut(s) 1453, 2032
BmrFI CCNGG 4 cut(s) 413, 1194, 1290, 1484
BmsI GCATC 8 cut(s) 174, 834, 1160, 1288, 1355, 1373, 1705, 1729
BpiI GAAGAC 3 cut(s) 225, 577, 1181
BpmI CTGGAG 2 cut(s) 1311, 1997
Bpu10I CCTNAGC 1 cut(s) 1989
BpuEI CTTGAG 3 cut(s) 764, 1072, 1766
BpuMI CCSGG 1 cut(s) 1194
Bsa29I ATCGAT 2 cut(s) 1367, 1658
BsaI GGTCTC 1 cut(s) 713
BsaJI CCNNGG 3 cut(s) 362, 1288, 1483
BsaWI WCCGGW 1 cut(s) 509
BsaXI ACNNNNNCTCC 2 cut(s) 1910, 1940
Bsc4I CCNNNNNNNGG 3 cut(s) 363, 629, 1639
Bse118I RCCGGY 2 cut(s) 622, 797
Bse1I ACTGG 3 cut(s) 1453, 1711, 1980
Bse3DI GCAATG 5 cut(s) 351, 854, 1106, 1359, 1413
BseBI CCWGG 3 cut(s) 413, 1290, 1484
BseCI ATCGAT 2 cut(s) 1367, 1658
BseDI CCNNGG 3 cut(s) 362, 1288, 1483
BseGI GGATG 2 cut(s) 892, 1272
BseLI CCNNNNNNNGG 3 cut(s) 363, 629, 1639
BseMI GCAATG 5 cut(s) 351, 854, 1106, 1359, 1413
BseMII CTCAG 2 cut(s) 183, 2003
BseNI ACTGG 3 cut(s) 1453, 1711, 1980
BseRI GAGGAG 1 cut(s) 1694
BsgI GTGCAG 1 cut(s) 1593
Bsh1236I CGCG 4 cut(s) 45, 177, 317, 372
BshFI GGCC 4 cut(s) 411, 614, 1265, 1994
BshVI ATCGAT 2 cut(s) 1367, 1658
BsiHKAI GWGCWC 1 cut(s) 1827
BsiSI CCGG 4 cut(s) 510, 623, 798, 1194
BslI CCNNNNNNNGG 3 cut(s) 363, 629, 1639
BsmAI GTCTC 4 cut(s) 195, 713, 1629, 1966
BsmBI CGTCTC 1 cut(s) 195
BsnI GGCC 4 cut(s) 411, 614, 1265, 1994
Bso31I GGTCTC 1 cut(s) 713
Bsp1286I GDGCHC 1 cut(s) 1827
Bsp143I GATC 7 cut(s) 33, 246, 421, 496, 802, 984, 1655
BspANI GGCC 4 cut(s) 411, 614, 1265, 1994
BspCNI CTCAG 2 cut(s) 182, 2002
BspDI ATCGAT 2 cut(s) 1367, 1658
BspFNI CGCG 4 cut(s) 45, 177, 317, 372
BspLI GGNNCC 2 cut(s) 1453, 2032
BspMAI CTGCAG 1 cut(s) 1917
BspPI GGATC 3 cut(s) 28, 491, 992
BspQI GCTCTTC 1 cut(s) 317
BspTNI GGTCTC 1 cut(s) 713
BsrBI CCGCTC 1 cut(s) 288
BsrDI GCAATG 5 cut(s) 351, 854, 1106, 1359, 1413
BsrFI RCCGGY 2 cut(s) 622, 797
BsrI ACTGG 3 cut(s) 1453, 1711, 1980
BssAI RCCGGY 2 cut(s) 622, 797
BssECI CCNNGG 3 cut(s) 362, 1288, 1483
BssMI GATC 7 cut(s) 33, 246, 421, 496, 802, 984, 1655
BssSI CACGAG 5 cut(s) 901, 1167, 1725, 1758, 1817
BssT1I CCWWGG 1 cut(s) 362
Bst2BI CACGAG 5 cut(s) 901, 1167, 1725, 1758, 1817
Bst2UI CCWGG 3 cut(s) 413, 1290, 1484
Bst4CI ACNGT 5 cut(s) 715, 1108, 1218, 1596, 1666
Bst6I CTCTTC 3 cut(s) 317, 425, 1065
BstC8I GCNNGC 6 cut(s) 175, 313, 347, 661, 814, 1827
BstDEI CTNAG 3 cut(s) 169, 1602, 1989
BstF5I GGATG 2 cut(s) 892, 1272
BstFNI CGCG 4 cut(s) 45, 177, 317, 372
BstHHI GCGC 6 cut(s) 47, 162, 179, 317, 372, 1816
BstKTI GATC 7 cut(s) 36, 249, 424, 499, 805, 987, 1658
BstMAI GTCTC 4 cut(s) 195, 713, 1629, 1966
BstMBI GATC 7 cut(s) 33, 246, 421, 496, 802, 984, 1655
BstMWI GCNNNNNNNGC 7 cut(s) 53, 114, 346, 1412, 1607, 1822, 1991
BstNI CCWGG 3 cut(s) 413, 1290, 1484
BstNSI RCATGY 1 cut(s) 349
BstSCI CCNGG 4 cut(s) 411, 1192, 1288, 1482
BstSFI CTRYAG 2 cut(s) 1665, 1913
BstUI CGCG 4 cut(s) 45, 177, 317, 372
BstV2I GAAGAC 3 cut(s) 225, 577, 1181
Bsu15I ATCGAT 2 cut(s) 1367, 1658
BsuI GTATCC 3 cut(s) 202, 291, 1388
BsuRI GGCC 4 cut(s) 411, 614, 1265, 1994
BsuTUI ATCGAT 2 cut(s) 1367, 1658
BtgZI GCGATG 1 cut(s) 252
BtsCI GGATG 2 cut(s) 892, 1272
BtsI GCAGTG 1 cut(s) 1243
BtsIMutI CAGTG 1 cut(s) 1243
Cac8I GCNNGC 6 cut(s) 175, 313, 347, 661, 814, 1827
CaiI CAGNNNCTG 1 cut(s) 1975
CfoI GCGC 6 cut(s) 47, 162, 179, 317, 372, 1816
Cfr10I RCCGGY 2 cut(s) 622, 797
ClaI ATCGAT 2 cut(s) 1367, 1658
CseI GACGC 2 cut(s) 361, 376
Csp6I GTAC 4 cut(s) 620, 1043, 1084, 1172
CspCI CAANNNNNGTGG 2 cut(s) 388, 423
CviAII CATG 3 cut(s) 346, 823, 1963
CviQI GTAC 4 cut(s) 620, 1043, 1084, 1172
DdeI CTNAG 3 cut(s) 169, 1602, 1989
DpnI GATC 7 cut(s) 35, 248, 423, 498, 804, 986, 1657
DpnII GATC 7 cut(s) 33, 246, 421, 496, 802, 984, 1655
DrdI GACNNNNNNGTC 1 cut(s) 73
DseDI GACNNNNNNGTC 1 cut(s) 73
EaeI YGGCCR 1 cut(s) 409
Eam1104I CTCTTC 3 cut(s) 317, 425, 1065
EarI CTCTTC 3 cut(s) 317, 425, 1065
EciI GGCGGA 2 cut(s) 110, 1764
Ecl136II GAGCTC 1 cut(s) 1825
Eco130I CCWWGG 1 cut(s) 362
Eco24I GRGCYC 1 cut(s) 1827
Eco31I GGTCTC 1 cut(s) 713
Eco53kI GAGCTC 1 cut(s) 1825
Eco57I CTGAAG 3 cut(s) 449, 1558, 1566
EcoICRI GAGCTC 1 cut(s) 1825
EcoRI GAATTC 1 cut(s) 1615
EcoRII CCWGG 3 cut(s) 411, 1288, 1482
EcoT14I CCWWGG 1 cut(s) 362
EcoT38I GRGCYC 1 cut(s) 1827
ErhI CCWWGG 1 cut(s) 362
Esp3I CGTCTC 1 cut(s) 195
FaeI CATG 3 cut(s) 349, 826, 1966
FalI AAGNNNNNCTT 2 cut(s) 1493, 1525
FatI CATG 3 cut(s) 345, 822, 1962
FbaI TGATCA 2 cut(s) 421, 802
FblI GTMKAC 2 cut(s) 203, 951
FokI GGATG 2 cut(s) 899, 1259
FriOI GRGCYC 1 cut(s) 1827
FspBI CTAG 2 cut(s) 654, 1200
FspI TGCGCA 1 cut(s) 161
GlaI GCGC 6 cut(s) 46, 161, 178, 316, 371, 1815
GsuI CTGGAG 2 cut(s) 1311, 1997
HaeIII GGCC 4 cut(s) 411, 614, 1265, 1994
HapII CCGG 4 cut(s) 510, 623, 798, 1194
HgaI GACGC 2 cut(s) 361, 376
HhaI GCGC 6 cut(s) 47, 162, 179, 317, 372, 1816
Hin1II CATG 3 cut(s) 349, 826, 1966
Hin6I GCGC 6 cut(s) 45, 160, 177, 315, 370, 1814
HinP1I GCGC 6 cut(s) 45, 160, 177, 315, 370, 1814
HincII GTYRAC 2 cut(s) 204, 1923
HindII GTYRAC 2 cut(s) 204, 1923
HindIII AAGCTT 1 cut(s) 532
HinfI GANTC 9 cut(s) 185, 224, 433, 756, 948, 1231, 1247, 1938, 1959
HpaII CCGG 4 cut(s) 510, 623, 798, 1194
HphI GGTGA 3 cut(s) 703, 812, 1307
Hpy166II GTNNAC 5 cut(s) 204, 449, 952, 1172, 1923
Hpy188III TCNNGA 7 cut(s) 304, 376, 391, 589, 743, 1653, 1725
Hpy8I GTNNAC 5 cut(s) 204, 449, 952, 1172, 1923
Hpy99I CGWCG 4 cut(s) 205, 208, 335, 1937
HpyCH4III ACNGT 5 cut(s) 715, 1108, 1218, 1596, 1666
HpyCH4IV ACGT 1 cut(s) 1925
HpyF10VI GCNNNNNNNGC 7 cut(s) 53, 114, 346, 1412, 1607, 1822, 1991
HpyF3I CTNAG 3 cut(s) 169, 1602, 1989
HpySE526I ACGT 1 cut(s) 1925
Hsp92II CATG 3 cut(s) 349, 826, 1966
HspAI GCGC 6 cut(s) 45, 160, 177, 315, 370, 1814
Ksp22I TGATCA 2 cut(s) 421, 802
Kzo9I GATC 7 cut(s) 33, 246, 421, 496, 802, 984, 1655
LguI GCTCTTC 1 cut(s) 317
LmnI GCTCC 3 cut(s) 345, 1822, 1982
LweI GCATC 8 cut(s) 174, 834, 1160, 1288, 1355, 1373, 1705, 1729
MaeI CTAG 2 cut(s) 654, 1200
MaeII ACGT 1 cut(s) 1925
MaeIII GTNAC 2 cut(s) 1465, 1709
MalI GATC 7 cut(s) 35, 248, 423, 498, 804, 986, 1657
MbiI CCGCTC 1 cut(s) 288
MboI GATC 7 cut(s) 33, 246, 421, 496, 802, 984, 1655
MhlI GDGCHC 1 cut(s) 1827
MlsI TGGCCA 1 cut(s) 411
MluNI TGGCCA 1 cut(s) 411
MlyI GAGTC 4 cut(s) 179, 442, 957, 1947
MmeI TCCRAC 4 cut(s) 733, 1090, 1864, 1956
Mox20I TGGCCA 1 cut(s) 411
MroXI GAANNNNTTC 1 cut(s) 326
MscI TGGCCA 1 cut(s) 411
MseI TTAA 7 cut(s) 483, 939, 1184, 1445, 1479, 1859, 2015
Msp20I TGGCCA 1 cut(s) 411
MspA1I CMGCKG 2 cut(s) 427, 1559
MspI CCGG 4 cut(s) 510, 623, 798, 1194
MspR9I CCNGG 4 cut(s) 413, 1194, 1290, 1484
MvaI CCWGG 3 cut(s) 413, 1290, 1484
MvnI CGCG 4 cut(s) 45, 177, 317, 372
MwoI GCNNNNNNNGC 7 cut(s) 53, 114, 346, 1412, 1607, 1822, 1991
NciI CCSGG 1 cut(s) 1194
NdeII GATC 7 cut(s) 33, 246, 421, 496, 802, 984, 1655
NlaIII CATG 3 cut(s) 349, 826, 1966
NlaIV GGNNCC 2 cut(s) 1453, 2032
NmuCI GTSAC 1 cut(s) 1465
NsbI TGCGCA 1 cut(s) 161
NspI RCATGY 1 cut(s) 349
PaeI GCATGC 1 cut(s) 349
PciSI GCTCTTC 1 cut(s) 317
PdmI GAANNNNTTC 1 cut(s) 326
PfeI GAWTC 5 cut(s) 224, 756, 1231, 1247, 1959
PflFI GACNNNGTC 1 cut(s) 1937
PleI GAGTC 4 cut(s) 179, 441, 956, 1946
PpsI GAGTC 4 cut(s) 179, 441, 956, 1946
PshBI ATTAAT 1 cut(s) 939
Psp124BI GAGCTC 1 cut(s) 1827
Psp6I CCWGG 3 cut(s) 411, 1288, 1482
PspGI CCWGG 3 cut(s) 411, 1288, 1482
PspN4I GGNNCC 2 cut(s) 1453, 2032
PstI CTGCAG 1 cut(s) 1917
PstNI CAGNNNCTG 1 cut(s) 1975
PsyI GACNNNGTC 1 cut(s) 1937
PvuII CAGCTG 2 cut(s) 427, 1559
RsaI GTAC 4 cut(s) 621, 1044, 1085, 1173
RsaNI GTAC 4 cut(s) 620, 1043, 1084, 1172
SacI GAGCTC 1 cut(s) 1827
SalI GTCGAC 1 cut(s) 202
SapI GCTCTTC 1 cut(s) 317
SaqAI TTAA 7 cut(s) 483, 939, 1184, 1445, 1479, 1859, 2015
Sau3AI GATC 7 cut(s) 33, 246, 421, 496, 802, 984, 1655
ScaI AGTACT 1 cut(s) 1085
SchI GAGTC 4 cut(s) 179, 442, 957, 1947
ScrFI CCNGG 4 cut(s) 413, 1194, 1290, 1484
SduI GDGCHC 1 cut(s) 1827
SfaNI GCATC 8 cut(s) 174, 834, 1160, 1288, 1355, 1373, 1705, 1729
SfcI CTRYAG 2 cut(s) 1665, 1913
SgrDI CGTCGACG 1 cut(s) 202
SmlI CTYRAG 3 cut(s) 743, 1087, 1781
SmoI CTYRAG 3 cut(s) 743, 1087, 1781
SphI GCATGC 1 cut(s) 349
SspMI CTAG 2 cut(s) 654, 1200
SstI GAGCTC 1 cut(s) 1827
StyD4I CCNGG 4 cut(s) 411, 1192, 1288, 1482
StyI CCWWGG 1 cut(s) 362
TaaI ACNGT 5 cut(s) 715, 1108, 1218, 1596, 1666
TaiI ACGT 1 cut(s) 1928
TatI WGTACW 2 cut(s) 1042, 1083
TauI GCSGC 4 cut(s) 45, 291, 1268, 1646
TfiI GAWTC 5 cut(s) 224, 756, 1231, 1247, 1959
Tru1I TTAA 7 cut(s) 483, 939, 1184, 1445, 1479, 1859, 2015
Tru9I TTAA 7 cut(s) 483, 939, 1184, 1445, 1479, 1859, 2015
TscAI CASTG 1 cut(s) 1243
TseFI GTSAC 1 cut(s) 1465
Tsp45I GTSAC 1 cut(s) 1465
TspDTI ATGAA 6 cut(s) 121, 718, 957, 1210, 1608, 1916
TspGWI ACGGA 4 cut(s) 183, 221, 1950, 1971
TspRI CASTG 1 cut(s) 1243
Tth111I GACNNNGTC 1 cut(s) 1937
VspI ATTAAT 1 cut(s) 939
XapI RAATTY 6 cut(s) 465, 475, 504, 602, 931, 1615
XceI RCATGY 1 cut(s) 349
XmiI GTMKAC 2 cut(s) 203, 951
XmnI GAANNNNTTC 1 cut(s) 326
XspI CTAG 2 cut(s) 654, 1200
ZrmI AGTACT 1 cut(s) 1085
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.