Rw6G011500

protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0001220
Physical Location & Seq
Reverse (-)
20207723 .. 20210635
2913 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G011500.1

Sequence Viewer

Length: 978 bp
ATGCTGACAGCATTGCTCGATAACATATATGGGAACTGGCTGGACATAATTTCTTCTACAAGAGGTATCACAAACATACAGTATGATGATGAGGTATGCTTAATTTATATGCATTCCTTTTGGGTTACTTCAATGTTGAAGGAAAGACTTGGAGTACAAAAGGAGAAAAAGCTTCGTATGGTTGATTACAGGAAATATTCTAAGGTTCGGAAATGGACTCTTGGATTATCAGTCGGTAAAGACAAGATAGCCATAATAAGAGCCTCAGGCAGTATTAGTCAATCAAAAAGATACAAAGCTGCTATTGTCCAAATTGACTGCCCTGGAGGTGATGCCCTTGCTTCTGATTTGATGTGGAGGGAAATCAAACTTTTGGCTGCATCAAAACCGGTCATTGCGTCAATGTCTGATGTGGCAGCAAGTGGAGGATACTATATGGCTATGGCAGCAGATGCCATTGTTGCAGAAAATCTAACCTTAACTAGTTCCATTGGAGTTGTCACAGGGAAGTTTGACCTTGGAAAACTGTGCGAGAAGATTGGCTTCAACAAAGAAATCATATCACGGGGAAAAATTTGCAGATCTGAACTCTTTGCTAAGTCTGCACAGAGTTCATATAAGCAATTTTGGGACAAGGCAGCTTCTTCCAGATCAATGAATAAAATGGAGGAAGTTGCACAAGGGAGAGTTTGGACTGGTAAAGATGCAGCTTTCAGGGGCTTAGTTGATGCTATTGGTGGACTCTGTCAGGCTGTTACATTAGTGGAACTTGCAAGACCCTCCCCTTCACTGCCAGAACTTTTAAGTGGCGTAGGGAGTACTTTGGTTGGAGTCTATCAAACGCTGAAGACACAGCTGCAAGACTTGACATTTTCTGATGGATCCCGGATGGATGGAATCATGTTTCAGAGACTGGAGGGAACTTCTAGTGCCAACCCCATCTTCAATTTGATAAGAGATTACCTTGGTTCCCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_S49

Protein Analysis

325

Amino Acids

35.66

Weight (kDa)

9.21

Isoelectric Point (pI)

33.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S49 PF01343 125 - 255 3.4e-29 Peptidase family S49
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 876, 889
AcsI RAATTY 1 cut(s) 573
AcuI CTGAAG 1 cut(s) 866
AfaI GTAC 2 cut(s) 156, 820
AgeI ACCGGT 1 cut(s) 388
AgsI TTSAA 4 cut(s) 132, 139, 547, 946
AhlI ACTAGT 1 cut(s) 482
AjnI CCWGG 1 cut(s) 322
AjuI GAANNNNNNNTTGG 2 cut(s) 926, 958
AluBI AGCT 5 cut(s) 172, 299, 641, 710, 856
AluI AGCT 5 cut(s) 172, 299, 641, 710, 856
Alw26I GTCTC 1 cut(s) 904
AlwI GGATC 2 cut(s) 876, 889
AlwNI CAGNNNCTG 1 cut(s) 913
ApeKI GCWGC 7 cut(s) 299, 377, 416, 446, 638, 707, 856
ApoI RAATTY 1 cut(s) 573
AsiGI ACCGGT 1 cut(s) 388
AsuC2I CCSGG 1 cut(s) 886
AsuHPI GGTGA 1 cut(s) 341
AxyI CCTNAGG 1 cut(s) 265
BamHI GGATCC 1 cut(s) 881
BbsI GAAGAC 1 cut(s) 854
BbvI GCAGC 7 cut(s) 286, 364, 428, 458, 650, 719, 843
BccI CCATC 4 cut(s) 872, 883, 887, 947
BciT130I CCWGG 1 cut(s) 324
BciVI GTATCC 1 cut(s) 422
BcnI CCSGG 1 cut(s) 886
BcoDI GTCTC 1 cut(s) 904
BcuI ACTAGT 1 cut(s) 482
BfaI CTAG 2 cut(s) 483, 927
BfuI GTATCC 1 cut(s) 422
BglII AGATCT 1 cut(s) 581
BisI GCNGC 7 cut(s) 300, 378, 417, 447, 639, 708, 857
BlsI GCNGC 7 cut(s) 301, 379, 418, 448, 640, 709, 858
BmcAI AGTACT 1 cut(s) 820
Bme1390I CCNGG 2 cut(s) 324, 886
BmiI GGNNCC 2 cut(s) 883, 970
BmrFI CCNGG 2 cut(s) 324, 886
BmsI GCATC 5 cut(s) 322, 389, 442, 694, 718
BpiI GAAGAC 1 cut(s) 854
BpmI CTGGAG 2 cut(s) 345, 935
BpuMI CCSGG 1 cut(s) 886
BsaJI CCNNGG 3 cut(s) 322, 517, 964
BsaWI WCCGGW 1 cut(s) 388
Bse118I RCCGGY 1 cut(s) 388
Bse1I ACTGG 3 cut(s) 41, 700, 918
Bse21I CCTNAGG 1 cut(s) 265
Bse3DI GCAATG 2 cut(s) 11, 393
BseBI CCWGG 1 cut(s) 324
BseDI CCNNGG 3 cut(s) 322, 517, 964
BseGI GGATG 2 cut(s) 894, 898
BseMI GCAATG 2 cut(s) 11, 393
BseMII CTCAG 1 cut(s) 279
BseNI ACTGG 3 cut(s) 41, 700, 918
BseXI GCAGC 7 cut(s) 286, 364, 428, 458, 650, 719, 843
BsgI GTGCAG 1 cut(s) 588
BshTI ACCGGT 1 cut(s) 388
BsiSI CCGG 2 cut(s) 389, 886
BslFI GGGAC 1 cut(s) 644
BsmAI GTCTC 1 cut(s) 904
BsmFI GGGAC 1 cut(s) 644
BsmI GAATGC 1 cut(s) 112
Bsp143I GATC 3 cut(s) 581, 650, 881
BspCNI CTCAG 1 cut(s) 278
BspLI GGNNCC 2 cut(s) 883, 970
BspPI GGATC 2 cut(s) 876, 889
BsrDI GCAATG 2 cut(s) 11, 393
BsrFI RCCGGY 1 cut(s) 388
BsrI ACTGG 3 cut(s) 41, 700, 918
BssAI RCCGGY 1 cut(s) 388
BssECI CCNNGG 3 cut(s) 322, 517, 964
BssMI GATC 3 cut(s) 581, 650, 881
BssT1I CCWWGG 2 cut(s) 517, 964
Bst2UI CCWGG 1 cut(s) 324
Bst4CI ACNGT 2 cut(s) 81, 528
BstAPI GCANNNNNTGC 1 cut(s) 452
BstDEI CTNAG 4 cut(s) 201, 265, 597, 721
BstF5I GGATG 2 cut(s) 894, 898
BstKTI GATC 3 cut(s) 584, 653, 884
BstMAI GTCTC 1 cut(s) 904
BstMBI GATC 3 cut(s) 581, 650, 881
BstMWI GCNNNNNNNGC 4 cut(s) 446, 452, 461, 602
BstNI CCWGG 1 cut(s) 324
BstSCI CCNGG 2 cut(s) 322, 884
BstV1I GCAGC 7 cut(s) 286, 364, 428, 458, 650, 719, 843
BstV2I GAAGAC 1 cut(s) 854
BstX2I RGATCY 2 cut(s) 581, 881
BstYI RGATCY 2 cut(s) 581, 881
Bsu36I CCTNAGG 1 cut(s) 265
BsuI GTATCC 1 cut(s) 422
BtsCI GGATG 2 cut(s) 894, 898
BtsI GCAGTG 1 cut(s) 788
BtsIMutI CAGTG 1 cut(s) 788
CaiI CAGNNNCTG 1 cut(s) 913
Cfr10I RCCGGY 1 cut(s) 388
CseI GACGC 1 cut(s) 387
Csp6I GTAC 2 cut(s) 155, 819
CspAI ACCGGT 1 cut(s) 388
CviAII CATG 1 cut(s) 901
CviQI GTAC 2 cut(s) 155, 819
DdeI CTNAG 4 cut(s) 201, 265, 597, 721
DpnI GATC 3 cut(s) 583, 652, 883
DpnII GATC 3 cut(s) 581, 650, 881
Eco130I CCWWGG 2 cut(s) 517, 964
Eco57I CTGAAG 1 cut(s) 866
Eco81I CCTNAGG 1 cut(s) 265
EcoRII CCWGG 1 cut(s) 322
EcoT14I CCWWGG 2 cut(s) 517, 964
EcoT22I ATGCAT 1 cut(s) 114
ErhI CCWWGG 2 cut(s) 517, 964
FaeI CATG 1 cut(s) 904
FalI AAGNNNNNCTT 2 cut(s) 527, 559
FaqI GGGAC 1 cut(s) 644
FatI CATG 1 cut(s) 900
Fnu4HI GCNGC 7 cut(s) 300, 378, 417, 447, 639, 708, 857
FokI GGATG 2 cut(s) 901, 905
Fsp4HI GCNGC 7 cut(s) 300, 378, 417, 447, 639, 708, 857
FspBI CTAG 2 cut(s) 483, 927
GluI GCNGC 7 cut(s) 300, 378, 417, 447, 639, 708, 857
GsuI CTGGAG 2 cut(s) 345, 935
HapII CCGG 2 cut(s) 389, 886
HgaI GACGC 1 cut(s) 387
Hin1II CATG 1 cut(s) 904
HindIII AAGCTT 1 cut(s) 170
HinfI GANTC 4 cut(s) 217, 741, 831, 897
HpaII CCGG 2 cut(s) 389, 886
HphI GGTGA 1 cut(s) 341
Hpy166II GTNNAC 1 cut(s) 740
Hpy188I TCNGA 6 cut(s) 210, 346, 409, 586, 877, 909
Hpy188III TCNNGA 1 cut(s) 648
Hpy8I GTNNAC 1 cut(s) 740
HpyAV CCTTC 2 cut(s) 133, 795
HpyCH4III ACNGT 2 cut(s) 81, 528
HpyCH4V TGCA 9 cut(s) 112, 380, 464, 579, 605, 677, 707, 773, 859
HpyF10VI GCNNNNNNNGC 4 cut(s) 446, 452, 461, 602
HpyF3I CTNAG 4 cut(s) 201, 265, 597, 721
Hsp92II CATG 1 cut(s) 904
Kzo9I GATC 3 cut(s) 581, 650, 881
Lsp1109I GCAGC 7 cut(s) 286, 364, 428, 458, 650, 719, 843
LweI GCATC 5 cut(s) 322, 389, 442, 694, 718
MaeI CTAG 2 cut(s) 483, 927
MaeIII GTNAC 3 cut(s) 124, 499, 754
MalI GATC 3 cut(s) 583, 652, 883
MboI GATC 3 cut(s) 581, 650, 881
MboII GAAGA 5 cut(s) 45, 547, 636, 859, 934
MflI RGATCY 2 cut(s) 581, 881
MluCI AATT 6 cut(s) 48, 102, 312, 573, 623, 946
MlyI GAGTC 3 cut(s) 211, 735, 840
MmeI TCCRAC 1 cut(s) 808
MnlI CCTC 9 cut(s) 56, 85, 274, 320, 351, 419, 661, 790, 910
Mph1103I ATGCAT 1 cut(s) 114
MseI TTAA 3 cut(s) 101, 479, 803
MspA1I CMGCKG 1 cut(s) 856
MspI CCGG 2 cut(s) 389, 886
MspR9I CCNGG 2 cut(s) 324, 886
Mva1269I GAATGC 1 cut(s) 112
MvaI CCWGG 1 cut(s) 324
MwoI GCNNNNNNNGC 4 cut(s) 446, 452, 461, 602
NciI CCSGG 1 cut(s) 886
NdeII GATC 3 cut(s) 581, 650, 881
NlaIII CATG 1 cut(s) 904
NlaIV GGNNCC 2 cut(s) 883, 970
NmuCI GTSAC 1 cut(s) 499
NsiI ATGCAT 1 cut(s) 114
PctI GAATGC 1 cut(s) 112
PfeI GAWTC 1 cut(s) 897
PflFI GACNNNGTC 1 cut(s) 744
PfoI TCCNGGA 1 cut(s) 884
PinAI ACCGGT 1 cut(s) 388
PkrI GCNGC 7 cut(s) 301, 379, 418, 448, 640, 709, 858
PleI GAGTC 3 cut(s) 211, 735, 839
PpsI GAGTC 3 cut(s) 211, 735, 839
Psp6I CCWGG 1 cut(s) 322
PspGI CCWGG 1 cut(s) 322
PspN4I GGNNCC 2 cut(s) 883, 970
PstNI CAGNNNCTG 1 cut(s) 913
PsuI RGATCY 2 cut(s) 581, 881
PsyI GACNNNGTC 1 cut(s) 744
PvuII CAGCTG 1 cut(s) 856
RsaI GTAC 2 cut(s) 156, 820
RsaNI GTAC 2 cut(s) 155, 819
SaqAI TTAA 3 cut(s) 101, 479, 803
SatI GCNGC 7 cut(s) 300, 378, 417, 447, 639, 708, 857
Sau3AI GATC 3 cut(s) 581, 650, 881
ScaI AGTACT 1 cut(s) 820
SchI GAGTC 3 cut(s) 211, 735, 840
ScrFI CCNGG 2 cut(s) 324, 886
SfaNI GCATC 5 cut(s) 322, 389, 442, 694, 718
SpeI ACTAGT 1 cut(s) 482
Sse9I AATT 6 cut(s) 48, 102, 312, 573, 623, 946
SspI AATATT 1 cut(s) 197
SspMI CTAG 2 cut(s) 483, 927
StyD4I CCNGG 2 cut(s) 322, 884
StyI CCWWGG 2 cut(s) 517, 964
TaaI ACNGT 2 cut(s) 81, 528
TaqI TCGA 1 cut(s) 18
TasI AATT 6 cut(s) 48, 102, 312, 573, 623, 946
TatI WGTACW 2 cut(s) 154, 818
TfiI GAWTC 1 cut(s) 897
Tru1I TTAA 3 cut(s) 101, 479, 803
Tru9I TTAA 3 cut(s) 101, 479, 803
TscAI CASTG 1 cut(s) 795
TseFI GTSAC 1 cut(s) 499
TseI GCWGC 7 cut(s) 299, 377, 416, 446, 638, 707, 856
Tsp45I GTSAC 1 cut(s) 499
TspDTI ATGAA 2 cut(s) 603, 671
TspRI CASTG 1 cut(s) 795
Tth111I GACNNNGTC 1 cut(s) 744
XapI RAATTY 1 cut(s) 573
XspI CTAG 2 cut(s) 483, 927
ZrmI AGTACT 1 cut(s) 820
Zsp2I ATGCAT 1 cut(s) 114
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.