Rh7DG063600

protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0001220
Physical Location & Seq
Reverse (-)
4953432 .. 4956332
2901 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG063600.1

Sequence Viewer

Length: 1323 bp
ATGAAATTTTGTTTTTCAGGTGTTCTTGAGAAAATTGGAGTTGAGCCACAAGTGGAAAGGATTGGTAAATATAAAAGTGCTGGTGACCAACTTGCGCGCACGAGCATGTCCGAAGAAAATTGTGAGATGCTGACAGCATTGCTCGATAACATATATGGGAACTGGCTGGACATAATTTCTTCTACAAGAGGAAAAAAGAGAGAAGATATTGAGAAGTTTGTTAATGAAGGGGTGTACCAAGTAGAAAAGCTGAAAGAAGAGGGCTGGATCACAAACATACAGTATGATGATGAGGTTACTTCAATGTTGAAGGAAAGACTTGGAGTACAAAAAGAGAAAAAGCTTCCTATGGTTGATTACAGGAAATATTCTAAGGTTCGGAAATGGACTCTTGGTTTATCAGGCGGTAAAGACCAGATAGCCATAATAAGAGCCTCAGGCAGTATTAGTCGTGTACGAGGTTCTTTTAGTCTACCTGGATCATCTATTGTTGGAGAACAGTTCATTGAGAAGATCCGTACTATAAGAGAATCAAAAAGATACAAGGCTGCTATTATCCGAATTGACAGCCCTGGAGGTGATGCCCTTGCTTCTGATTTGATGTGGAGGGAAATCAAACTTTTGGCTGCATCAAAGCCGGTCATTGCATCAATGTCTGATGTGGCAGCAAGTGGAGGATACTATATGGCTATGGCAGCAGATGCCATTGTTGCAGAAAATCTAACCTTAACTGGTTCCATTGGAGTTGTCACAGGGAAGTTTAACCTGGGAAAACTGTATGAGAAGATTGGGTTCAACAAGGAAATCATATCAAGGGGGAAATTTGCGGAGGTTCTTGCAGCTGAACAACGACCCTTCAAACCAGAGGAAGCTGAACTCTTTGCTAAGTCTGCACAGAATTCGTATAAGCAATTTCGGGACAAGGCAGCTTCTTCCAGATCAATGACTGCAGATAAAATGGAGGAAGTTGCACAAGGGAGAGTTTGGGCTGGTAAAGATGCAGCTTCGAGGGGTTTAGTTGATGCCATTGGTGGACTTTCTCGGGCTGTTGCAATAGCAAAGCTCAAGGCAAATATACCACAAGACACAGAGGTTACACTTGTGGAGCTTGCAAGACCCTCCCCTTCACTGCCAGAACTTTTAAGTGGCGTAGGGAGTACTCTGGTTGGAGTCGAGCAAACGATGAAGACACTGTTGCAAGACTTAACATTTTCTGATGGAGTTCAAGCCCGGATGGATGGAATCTTATTTCAGAGACTGGAGGGAGCTTCTAATGCCAACCCCATCTTCAATTTGATAAGAGATTACCTTGGTTCCCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_S49

Protein Analysis

440

Amino Acids

48.29

Weight (kDa)

8.96

Isoelectric Point (pI)

32.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S49 PF01343 4 - 109 1e-12 Peptidase family S49
Peptidase_S49 PF01343 208 - 358 2.2e-42 Peptidase family S49
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 472
AccII CGCG 1 cut(s) 97
AciI CCGC 2 cut(s) 405, 827
AclWI GGATC 3 cut(s) 275, 487, 508
AcsI RAATTY 3 cut(s) 5, 821, 898
AfaI GTAC 5 cut(s) 236, 327, 456, 520, 1159
AgsI TTSAA 6 cut(s) 303, 310, 796, 859, 1226, 1291
AjnI CCWGG 3 cut(s) 475, 571, 765
AjuI GAANNNNNNNTTGG 2 cut(s) 1271, 1303
AluBI AGCT 9 cut(s) 250, 343, 842, 872, 929, 1004, 1063, 1108, 1268
AluI AGCT 9 cut(s) 250, 343, 842, 872, 929, 1004, 1063, 1108, 1268
Alw26I GTCTC 1 cut(s) 1249
AlwI GGATC 3 cut(s) 275, 487, 508
AlwNI CAGNNNCTG 1 cut(s) 1258
Ama87I CYCGRG 1 cut(s) 1041
ApeKI GCWGC 7 cut(s) 548, 626, 665, 695, 839, 926, 1001
ApoI RAATTY 3 cut(s) 5, 821, 898
AspLEI GCGC 2 cut(s) 97, 99
AsuC2I CCSGG 1 cut(s) 1231
AsuHPI GGTGA 2 cut(s) 95, 590
AvaI CYCGRG 1 cut(s) 1041
AxyI CCTNAGG 1 cut(s) 436
BauI CACGAG 1 cut(s) 100
BbsI GAAGAC 1 cut(s) 1193
BbvI GCAGC 7 cut(s) 535, 613, 677, 707, 851, 938, 1013
BccI CCATC 4 cut(s) 1211, 1228, 1232, 1292
BcgI CGANNNNNNTGC 2 cut(s) 882, 916
BciT130I CCWGG 3 cut(s) 477, 573, 767
BciVI GTATCC 1 cut(s) 671
BcnI CCSGG 1 cut(s) 1231
BcoDI GTCTC 1 cut(s) 1249
BfmI CTRYAG 1 cut(s) 948
BfuI GTATCC 1 cut(s) 671
BisI GCNGC 7 cut(s) 549, 627, 666, 696, 840, 927, 1002
BlsI GCNGC 7 cut(s) 550, 628, 667, 697, 841, 928, 1003
BmcAI AGTACT 1 cut(s) 1159
Bme1390I CCNGG 4 cut(s) 477, 573, 767, 1231
BmeT110I CYCGRG 1 cut(s) 1041
BmiI GGNNCC 2 cut(s) 736, 1315
BmrFI CCNGG 4 cut(s) 477, 573, 767, 1231
BmsI GCATC 7 cut(s) 117, 571, 638, 656, 691, 988, 1012
BpiI GAAGAC 1 cut(s) 1193
BpmI CTGGAG 2 cut(s) 594, 1280
BpuEI CTTGAG 2 cut(s) 47, 1049
BpuMI CCSGG 1 cut(s) 1231
BsaJI CCNNGG 3 cut(s) 571, 766, 1309
Bse118I RCCGGY 1 cut(s) 637
Bse1I ACTGG 3 cut(s) 167, 736, 1263
Bse21I CCTNAGG 1 cut(s) 436
Bse3DI GCAATG 2 cut(s) 137, 642
BseBI CCWGG 3 cut(s) 477, 573, 767
BseDI CCNNGG 3 cut(s) 571, 766, 1309
BseGI GGATG 2 cut(s) 1239, 1243
BseMI GCAATG 2 cut(s) 137, 642
BseMII CTCAG 1 cut(s) 450
BseNI ACTGG 3 cut(s) 167, 736, 1263
BsePI GCGCGC 1 cut(s) 95
BseXI GCAGC 7 cut(s) 535, 613, 677, 707, 851, 938, 1013
BsgI GTGCAG 1 cut(s) 876
Bsh1236I CGCG 1 cut(s) 97
BsiHKCI CYCGRG 1 cut(s) 1041
BsiSI CCGG 2 cut(s) 638, 1231
BslFI GGGAC 1 cut(s) 932
BsmAI GTCTC 1 cut(s) 1249
BsmFI GGGAC 1 cut(s) 932
BsoBI CYCGRG 1 cut(s) 1041
Bsp143I GATC 4 cut(s) 267, 479, 513, 938
BspACI CCGC 2 cut(s) 405, 827
BspCNI CTCAG 1 cut(s) 449
BspFNI CGCG 1 cut(s) 97
BspLI GGNNCC 2 cut(s) 736, 1315
BspMAI CTGCAG 1 cut(s) 952
BspPI GGATC 3 cut(s) 275, 487, 508
BsrDI GCAATG 2 cut(s) 137, 642
BsrFI RCCGGY 1 cut(s) 637
BsrI ACTGG 3 cut(s) 167, 736, 1263
BssAI RCCGGY 1 cut(s) 637
BssECI CCNNGG 3 cut(s) 571, 766, 1309
BssHII GCGCGC 1 cut(s) 95
BssMI GATC 4 cut(s) 267, 479, 513, 938
BssSI CACGAG 1 cut(s) 100
BssT1I CCWWGG 1 cut(s) 1309
Bst2BI CACGAG 1 cut(s) 100
Bst2UI CCWGG 3 cut(s) 477, 573, 767
Bst4CI ACNGT 4 cut(s) 282, 501, 777, 1194
Bst6I CTCTTC 1 cut(s) 252
BstAPI GCANNNNNTGC 1 cut(s) 701
BstC8I GCNNGC 2 cut(s) 97, 1110
BstDEI CTNAG 3 cut(s) 372, 436, 885
BstEII GGTNACC 1 cut(s) 83
BstF5I GGATG 2 cut(s) 1239, 1243
BstFNI CGCG 1 cut(s) 97
BstHHI GCGC 2 cut(s) 97, 99
BstKTI GATC 4 cut(s) 270, 482, 516, 941
BstMAI GTCTC 1 cut(s) 1249
BstMBI GATC 4 cut(s) 267, 479, 513, 938
BstMWI GCNNNNNNNGC 5 cut(s) 695, 701, 710, 890, 1274
BstNI CCWGG 3 cut(s) 477, 573, 767
BstNSI RCATGY 1 cut(s) 109
BstPI GGTNACC 1 cut(s) 83
BstSCI CCNGG 4 cut(s) 475, 571, 765, 1229
BstSFI CTRYAG 1 cut(s) 948
BstUI CGCG 1 cut(s) 97
BstV1I GCAGC 7 cut(s) 535, 613, 677, 707, 851, 938, 1013
BstV2I GAAGAC 1 cut(s) 1193
BstX2I RGATCY 1 cut(s) 513
BstYI RGATCY 1 cut(s) 513
Bsu36I CCTNAGG 1 cut(s) 436
BsuI GTATCC 1 cut(s) 671
BtsCI GGATG 2 cut(s) 1239, 1243
BtsI GCAGTG 1 cut(s) 1127
BtsIMutI CAGTG 2 cut(s) 1127, 1190
Cac8I GCNNGC 2 cut(s) 97, 1110
CaiI CAGNNNCTG 1 cut(s) 1258
CfoI GCGC 2 cut(s) 97, 99
Cfr10I RCCGGY 1 cut(s) 637
Csp6I GTAC 5 cut(s) 235, 326, 455, 519, 1158
CviAII CATG 1 cut(s) 106
CviQI GTAC 5 cut(s) 235, 326, 455, 519, 1158
DdeI CTNAG 3 cut(s) 372, 436, 885
DpnI GATC 4 cut(s) 269, 481, 515, 940
DpnII GATC 4 cut(s) 267, 479, 513, 938
Eam1104I CTCTTC 1 cut(s) 252
EarI CTCTTC 1 cut(s) 252
Eco130I CCWWGG 1 cut(s) 1309
Eco81I CCTNAGG 1 cut(s) 436
Eco88I CYCGRG 1 cut(s) 1041
Eco91I GGTNACC 1 cut(s) 83
EcoO65I GGTNACC 1 cut(s) 83
EcoRI GAATTC 1 cut(s) 898
EcoRII CCWGG 3 cut(s) 475, 571, 765
EcoT14I CCWWGG 1 cut(s) 1309
ErhI CCWWGG 1 cut(s) 1309
FaeI CATG 1 cut(s) 109
FaqI GGGAC 1 cut(s) 932
FatI CATG 1 cut(s) 105
FblI GTMKAC 1 cut(s) 472
Fnu4HI GCNGC 7 cut(s) 549, 627, 666, 696, 840, 927, 1002
FokI GGATG 2 cut(s) 1246, 1250
Fsp4HI GCNGC 7 cut(s) 549, 627, 666, 696, 840, 927, 1002
GlaI GCGC 2 cut(s) 96, 98
GluI GCNGC 7 cut(s) 549, 627, 666, 696, 840, 927, 1002
GsuI CTGGAG 2 cut(s) 594, 1280
HapII CCGG 2 cut(s) 638, 1231
HhaI GCGC 2 cut(s) 97, 99
Hin1II CATG 1 cut(s) 109
Hin6I GCGC 2 cut(s) 95, 97
HinP1I GCGC 2 cut(s) 95, 97
HindIII AAGCTT 1 cut(s) 341
HinfI GANTC 4 cut(s) 388, 530, 1170, 1242
HpaII CCGG 2 cut(s) 638, 1231
HphI GGTGA 2 cut(s) 95, 590
Hpy166II GTNNAC 4 cut(s) 235, 455, 473, 1034
Hpy188I TCNGA 7 cut(s) 112, 381, 560, 595, 658, 1216, 1254
Hpy188III TCNNGA 3 cut(s) 26, 917, 936
Hpy8I GTNNAC 4 cut(s) 235, 455, 473, 1034
HpyAV CCTTC 4 cut(s) 221, 304, 865, 1134
HpyCH4III ACNGT 4 cut(s) 282, 501, 777, 1194
HpyF10VI GCNNNNNNNGC 5 cut(s) 695, 701, 710, 890, 1274
HpyF3I CTNAG 3 cut(s) 372, 436, 885
Hsp92II CATG 1 cut(s) 109
HspAI GCGC 2 cut(s) 95, 97
Kzo9I GATC 4 cut(s) 267, 479, 513, 938
LmnI GCTCC 2 cut(s) 1105, 1265
Lsp1109I GCAGC 7 cut(s) 535, 613, 677, 707, 851, 938, 1013
LweI GCATC 7 cut(s) 117, 571, 638, 656, 691, 988, 1012
MaeIII GTNAC 4 cut(s) 83, 295, 748, 1093
MalI GATC 4 cut(s) 269, 481, 515, 940
MboI GATC 4 cut(s) 267, 479, 513, 938
MboII GAAGA 9 cut(s) 125, 171, 215, 269, 523, 796, 924, 1198, 1279
MflI RGATCY 1 cut(s) 513
MluCI AATT 9 cut(s) 5, 33, 118, 174, 561, 821, 898, 911, 1291
MlyI GAGTC 2 cut(s) 382, 1179
MmeI TCCRAC 2 cut(s) 472, 1147
MseI TTAA 5 cut(s) 222, 728, 762, 1142, 1205
MslI CAYNNNNRTG 1 cut(s) 104
MspA1I CMGCKG 1 cut(s) 842
MspI CCGG 2 cut(s) 638, 1231
MspR9I CCNGG 4 cut(s) 477, 573, 767, 1231
MvaI CCWGG 3 cut(s) 477, 573, 767
MvnI CGCG 1 cut(s) 97
MwoI GCNNNNNNNGC 5 cut(s) 695, 701, 710, 890, 1274
NciI CCSGG 1 cut(s) 1231
NdeII GATC 4 cut(s) 267, 479, 513, 938
NlaIII CATG 1 cut(s) 109
NlaIV GGNNCC 2 cut(s) 736, 1315
NmuCI GTSAC 2 cut(s) 83, 748
NspI RCATGY 1 cut(s) 109
PauI GCGCGC 1 cut(s) 95
PfeI GAWTC 2 cut(s) 530, 1242
PkrI GCNGC 7 cut(s) 550, 628, 667, 697, 841, 928, 1003
PleI GAGTC 2 cut(s) 382, 1178
PpsI GAGTC 2 cut(s) 382, 1178
Psp6I CCWGG 3 cut(s) 475, 571, 765
PspEI GGTNACC 1 cut(s) 83
PspGI CCWGG 3 cut(s) 475, 571, 765
PspN4I GGNNCC 2 cut(s) 736, 1315
PstI CTGCAG 1 cut(s) 952
PstNI CAGNNNCTG 1 cut(s) 1258
PsuI RGATCY 1 cut(s) 513
PteI GCGCGC 1 cut(s) 95
PvuII CAGCTG 1 cut(s) 842
RsaI GTAC 5 cut(s) 236, 327, 456, 520, 1159
RsaNI GTAC 5 cut(s) 235, 326, 455, 519, 1158
RseI CAYNNNNRTG 1 cut(s) 104
SaqAI TTAA 5 cut(s) 222, 728, 762, 1142, 1205
SatI GCNGC 7 cut(s) 549, 627, 666, 696, 840, 927, 1002
Sau3AI GATC 4 cut(s) 267, 479, 513, 938
ScaI AGTACT 1 cut(s) 1159
SchI GAGTC 2 cut(s) 382, 1179
ScrFI CCNGG 4 cut(s) 477, 573, 767, 1231
SfaNI GCATC 7 cut(s) 117, 571, 638, 656, 691, 988, 1012
SfcI CTRYAG 1 cut(s) 948
SmiMI CAYNNNNRTG 1 cut(s) 104
SmlI CTYRAG 2 cut(s) 26, 1064
SmoI CTYRAG 2 cut(s) 26, 1064
Sse9I AATT 9 cut(s) 5, 33, 118, 174, 561, 821, 898, 911, 1291
SsiI CCGC 2 cut(s) 405, 827
SspI AATATT 1 cut(s) 368
StyD4I CCNGG 4 cut(s) 475, 571, 765, 1229
StyI CCWWGG 1 cut(s) 1309
TaaI ACNGT 4 cut(s) 282, 501, 777, 1194
TaqI TCGA 3 cut(s) 144, 1007, 1173
TasI AATT 9 cut(s) 5, 33, 118, 174, 561, 821, 898, 911, 1291
TatI WGTACW 2 cut(s) 325, 1157
TfiI GAWTC 2 cut(s) 530, 1242
Tru1I TTAA 5 cut(s) 222, 728, 762, 1142, 1205
Tru9I TTAA 5 cut(s) 222, 728, 762, 1142, 1205
TscAI CASTG 2 cut(s) 1134, 1197
TseFI GTSAC 2 cut(s) 83, 748
TseI GCWGC 7 cut(s) 548, 626, 665, 695, 839, 926, 1001
Tsp45I GTSAC 2 cut(s) 83, 748
TspDTI ATGAA 4 cut(s) 17, 240, 493, 1199
TspGWI ACGGA 1 cut(s) 506
TspRI CASTG 2 cut(s) 1134, 1197
XapI RAATTY 3 cut(s) 5, 821, 898
XceI RCATGY 1 cut(s) 109
XmiI GTMKAC 1 cut(s) 472
ZrmI AGTACT 1 cut(s) 1159
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.