Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0001459

Physical Location & Seq

Genomic Coordinates Reverse (-)

3810784 .. 3816831

Gene Structure 6048 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1054400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2583 bp

ATGATAGATGCTCTTGTTAGGCATTATGATGTTAGAGATAGGTGTTTTAAGATTAATAATCAAAATTTATTTTTCGGATTAGAAGATGTGCTTTTAATCACTGGGCTTCCAGTAGAAGGGACCCCGGTGATAAAGTCAACAACAAACATTCATGCCCTTGAAAACTTGTTAGGGGGAGTGCCTGAAAGTGAGTTGAAGGATAAATCAAATGCGGTGAACCTTAGTTGGTTACAAAAAGAGTTTGAGCATGTACCTGAAAATGTAGATGCCGATAGATTTTCCTGTCATGTTAGAGGATTTGTCCTCTACATATTAGGCACGGTAATGGTTCCCAGTTTAGACCATGTGCACGTGAATCTGGGCTACTTGGGCTGCATGTCCGAGATAGGTGAGATAAAGAAGTATGCATGGGGTGTTGCACTGCTTTCCCATGTGCACTATAGTCTAGAGAGTCATCATAAAAGAAGGAAAAATGCTATTGGCAGCCACTTGTTCTTCCTAATGGTGTGGGCATTGGAGCATATCCCTGCCTTTGTGACGGAATTTTTCGGGAATGAGTTAGACACCTATCCCAGCGAGTTCCCGCTGTCTTGTGCTTGGGGAAAATTGTTTCATAATGTGCTTTCTAATAGGCACAAAGTGAGGGACTTTAATCGGCACTTGGAGAACCTTTGGGAAAATGAAAATTCGGTTACTTGGCAGCCTTATATGAGGCTTGCTGACAATTTCCTCCCCGAAGAGATTAGGGGGCAGCGGCGGATGGGAAGCTGCCGGGTGATTTTGATTCACCCGATCCACAGGTGCCCCCTCATATTCCATCGTCCTGATCTTTGCCCAAGGAAACTTGGGTTTAGAGAGGCCGATGTGACTGCCTGTTCCCCAATGCTTTCTGATCGCACAAGATTTCGAAGTTGGAAACTTCATCTAGAAGAGTGGGAGAAGTTGGAGACATGCTTTGTGAGGAACCGGTTAGATCAACAAGAAACGAACCTGAGAGAAGGCGGAACCAAATCTGGAGGATTCGAAAACATAGAAAGAATCCATATTACAGTACAAGAATGGTCTGAGGCAGAAATTACAGCAACAGCAGCAGCTGCTCTATCTGCATCTGCAACTCAGGTCATTGAACGAGCAGCAAATCTAGATGTTCCATCTGCATCTATACCTGTTCCGTCTGCATCTATACCTGTTCCATCTACATCAGCACCTCAAGATGTTCCAGAACCAGGAGCACATGTTCCATTTGCATCTGCAACTCAAGACGTTGCAGAACAAGTAGCACCGCCGCCGGTCGTCGATTCATCGTCTTCCTTCATCAAAAACTCTGAATCCAAAGACAACCCATTGTTATCGCCGTCACCACCCAAAATCCCAATTGTATGGCCGCCCGACGGAAAGCTCAGCCTTGAATGGGTTCGAAACCTCATGTCCACTATGGATTGGGCTTCTAGAAATCTTGACCCGATGAAATTTCCGGACATGTTTCCTATGGAGGTTTTCGATAGCTTGGTCATCAGCGCCTCCAATATCCTCCACAAAGAGGCAAATTGCGTTTTAATCGACCGCTTAGGCTCGGAATCCACTGTTGTCGTTGTTGGAGACATTCATGGTCAGCTGCATGATCTCCTTTTCCTTCTAAATGATGCTGGATTTCCTTCAGAAAATCGATTATTCGTCTTCAATGGCGATTATGTTGATAGAGGGGCTTGGGGGCTCGAAACCTTCTTGATTTTATTGGCATGGAAAGTGTTTTTGCCCAAGAGCGTTTATCTTCTTAGAGGAAATCATGAATCCAAATATTGCACTTCCGTTTATGGATTTGAGAAGGAAGTGTTGACAAAGTATGGAGACAAAGGGAAAGATGTATACCGACAATGTCTCTGGTGCTTTAAGCGTCTCCCTTTGACATCCATTATCGGGAAGTATGTATATACTGCTCATGGTGGGCTGTTTCGCAGCATGGCGGCATACCCCAAGAGATCAAAAGGAAAGAAGAGAAGGAGAATAGCTTTCGATCCTGAACTGAGCAGTCCATTGTCTCTTGGCACATTTGAAGAATTAAAGAAGGCTCAAAGATCGGTTTTGGATCCTTCGTGTGAAGGTTCGAACTTGATTCCTGGTGACGTGCTATGGTCAGATCCCTCCATGGATCCCGGTCTTTCTTTGAACACAGAGAGAGGCATTGGTTTGCTTTGGGGCCCTGACTGCACTGAGGATTTTCTCAAGAAGTCTCAACTCAAGTTGATTATTAGAACGCATGAAGGCCCTGATGCGCGGGACAAGAGGCAAAGGCCAGGTCTTGAGGGAATGGATAACGGGTTCACCATAGATCACATTGTGGAGTCTGGAAAGCTCATTACTTTGTTTAGTGCTCCAGACTACCCACAATTTCAGGGGACAGGAGATAGGTGCAGAAATAAAGGAGCTTACATCGTTCTGGAACCCCCAAATTTTGATAATCCTGTTTTTCATAGCTTTGAAGCAGTCACTCCAAGACCTCAGGCGAATCCCTTCTATGATTTTGAAGAAGTGGCCGATTCTGATCAAGAGTTGGACTTCGCATCGATGCAGACTAGCCCGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004721 GO:0004722 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006464 GO:0006470 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009266 GO:0009314 GO:0009408 GO:0009416 GO:0009628 GO:0009637 GO:0009785 GO:0009987 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0019538 GO:0023052 GO:0030522 GO:0036211 GO:0042578 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071214 GO:0071478 GO:0071482 GO:0071483 GO:0071704 GO:0104004 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04010 ko04922 map04010 map04922

Pfam Domains

Protein Families

Metallophos PMD

Protein Analysis

861

Amino Acids

97.0

Weight (kDa)

5.88

Isoelectric Point (pI)

47.84

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DUF7745	PF24924	2 - 181	3e-07	Domain of unknown function (DUF7745)
PMD	PF10536	27 - 254	1.4e-28	Plant mobile domain
Metallophos	PF00149	528 - 754	1.7e-28	Calcineurin-like phosphoesterase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001459)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g02840 FvH4_4g28291 FvH4_4g28291 FvH4_5g29581
malus_domestica	MD10G1009900.v1.1 MD13G1054400.v1.1
prunus_persica	Prupe.8G013700_v2.0.a1 Prupe.8G013700_v2.0.a1 Prupe.8G013900_v2.0.a1 Prupe.8G014000_v2.0.a1
pyrus_communis	pycom10g00610 pycom13g04830 pycom14g08210
rosa_laevigata	RLG00000013271
rosa_multiflora	Rmu_co8191550.1_g000001 Rmu_co8290979.1_g000001 Rmu_sc0000056.1_g000004 Rmu_sc0000834.1_g000019 Rmu_sc0004145.1_g000004 Rmu_sc0004145.1_g000014 Rmu_sc0008004.1_g000008 Rmu_sc0009291.1_g000008 Rmu_sc0042239.1_g000001 Rmu_ssc0000189.1_g000023 Rmu_ssc0000255.1_g000006
rosa_roxburghii	Rroxscaffold_5G00350480 Rroxscaffold_5G00350490
rosa_rugosa	Rorug01G0106300 Rorug01G0106300 Rorug01G0106400 Rorug01G0210200
rosa_samantha	Rh1AG123300 Rh1AG225300 Rh1BG097100 Rh1BG098700 Rh1BG193200 Rh1CG210100 Rh1DG128500 Rh1DG221500 Rh2AG598100 Rh2AG598200 Rh5BG336600 Rh6AG174000 Rh7AG329800 Rh7AG336300 Rh7AG336400 Rh7CG354000 Rh7CG354100 Rh7DG332600
rosa_wichuraiana	Rw0G011920 Rw1G019370 Rw6G014810

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	801
AccI	GTMKAC	1 cut(s)	1866
AccII	CGCG	1 cut(s)	2275
AccIII	TCCGGA	1 cut(s)	1474
AclWI	GGATC	7 cut(s)	787, 2009, 2081, 2094, 2132, 2144, 2157
AcoI	YGGCCR	2 cut(s)	1382, 2532
AcsI	RAATTY	5 cut(s)	64, 542, 685, 1469, 2449
AcuI	CTGAAG	1 cut(s)	1641
AcvI	CACGTG	1 cut(s)	352
AdeI	CACNNNGTG	2 cut(s)	640, 2338
AfaI	GTAC	2 cut(s)	252, 1053
AfiI	CCNNNNNNNGG	6 cut(s)	116, 1226, 1391, 1411, 1540, 1917
AflIII	ACRYGT	2 cut(s)	1234, 1479
AgeI	ACCGGT	1 cut(s)	966
AgsI	TTSAA	9 cut(s)	161, 196, 1127, 1409, 1681, 2054, 2167, 2480, 2525
AjiI	CACGTC	1 cut(s)	2125
AjnI	CCWGG	3 cut(s)	1225, 2116, 2293
AloI	GAACNNNNNNTCC	4 cut(s)	1221, 1253, 2303, 2335
AluBI	AGCT	9 cut(s)	768, 1094, 1399, 1506, 1615, 2009, 2353, 2426, 2475
AluI	AGCT	9 cut(s)	768, 1094, 1399, 1506, 1615, 2009, 2353, 2426, 2475
Alw21I	GWGCWC	4 cut(s)	351, 438, 1234, 2374
Alw26I	GTCTC	7 cut(s)	941, 1593, 1842, 1883, 1901, 2043, 2235
Alw44I	GTGCAC	2 cut(s)	347, 434
AlwI	GGATC	7 cut(s)	787, 2009, 2081, 2094, 2132, 2144, 2157
AlwNI	CAGNNNCTG	1 cut(s)	1094
Aor13HI	TCCGGA	1 cut(s)	1474
AoxI	GGCC	6 cut(s)	858, 1382, 2197, 2263, 2291, 2532
ApaI	GGGCCC	1 cut(s)	2201
ApaLI	GTGCAC	2 cut(s)	347, 434
ApoI	RAATTY	5 cut(s)	64, 542, 685, 1469, 2449
ArsI	GACNNNNNNTTYG	2 cut(s)	2281, 2313
AseI	ATTAAT	1 cut(s)	54
AsiGI	ACCGGT	1 cut(s)	966
Asp700I	GAANNNNTTC	2 cut(s)	1413, 2510
AspLEI	GCGC	2 cut(s)	1520, 2275
AspS9I	GGNCC	4 cut(s)	120, 2197, 2198, 2264
AsuC2I	CCSGG	3 cut(s)	125, 773, 2154
AsuHPI	GGTGA	8 cut(s)	139, 226, 401, 779, 787, 1350, 2132, 2314
AsuII	TTCGAA	4 cut(s)	907, 1023, 1417, 2105
AvaII	GGWCC	1 cut(s)	120
AxyI	CCTNAGG	1 cut(s)	2499
BaeGI	GKGCMC	4 cut(s)	351, 438, 806, 2201
BamHI	GGATCC	2 cut(s)	2086, 2149
BanI	GGYRCC	1 cut(s)	801
BanII	GRGCYC	2 cut(s)	1716, 2201
BbrPI	CACGTG	1 cut(s)	352
BbsI	GAAGAC	2 cut(s)	1299, 1669
Bbv12I	GWGCWC	4 cut(s)	351, 438, 1234, 2374
BccI	CCATC	4 cut(s)	754, 825, 1159, 1201
BceAI	ACGGC	1 cut(s)	1339
BcgI	CGANNNNNNTGC	2 cut(s)	851, 885
BciT130I	CCWGG	3 cut(s)	1227, 2118, 2295
BclI	TGATCA	1 cut(s)	2542
BcnI	CCSGG	3 cut(s)	125, 773, 2154
BcoDI	GTCTC	7 cut(s)	941, 1593, 1842, 1883, 1901, 2043, 2235
BfaI	CTAG	5 cut(s)	446, 926, 1142, 1449, 2574
BfmI	CTRYAG	1 cut(s)	439
BfoI	RGCGCY	1 cut(s)	1521
BlpI	GCTNAGC	1 cut(s)	1400
Bme1390I	CCNGG	6 cut(s)	125, 773, 1227, 2118, 2154, 2295
Bme18I	GGWCC	1 cut(s)	120
BmgBI	CACGTC	1 cut(s)	2125
BmgT120I	GGNCC	4 cut(s)	120, 2197, 2198, 2264
BmrFI	CCNGG	6 cut(s)	125, 773, 1227, 2118, 2154, 2295
BmrI	ACTGGG	2 cut(s)	111, 327
BmsI	GCATC	9 cut(s)	256, 1115, 1166, 1187, 1256, 1633, 2260, 2556, 2570
BmuI	ACTGGG	2 cut(s)	111, 327
BpiI	GAAGAC	2 cut(s)	1299, 1669
BpmI	CTGGAG	2 cut(s)	1035, 2358
Bpu10I	CCTNAGC	1 cut(s)	1567
Bpu1102I	GCTNAGC	1 cut(s)	1400
Bpu14I	TTCGAA	4 cut(s)	907, 1023, 1417, 2105
BpuEI	CTTGAG	5 cut(s)	1194, 1242, 2207, 2222, 2321
BpuMI	CCSGG	3 cut(s)	125, 773, 2154
Bsa29I	ATCGAT	2 cut(s)	1666, 2564
BsaAI	YACGTR	1 cut(s)	352
BsaBI	GATNNNNATC	1 cut(s)	2541
BsaJI	CCNNGG	3 cut(s)	123, 836, 2145
BsaWI	WCCGGW	2 cut(s)	966, 1474
BsaXI	ACNNNNNCTCC	2 cut(s)	1221, 1251
Bsc4I	CCNNNNNNNGG	6 cut(s)	116, 1226, 1391, 1411, 1540, 1917
Bse118I	RCCGGY	2 cut(s)	966, 1288
Bse1I	ACTGG	3 cut(s)	106, 110, 333
Bse21I	CCTNAGG	1 cut(s)	2499
Bse8I	GATNNNNATC	1 cut(s)	2541
BseAI	TCCGGA	1 cut(s)	1474
BseBI	CCWGG	3 cut(s)	1227, 2118, 2295
BseCI	ATCGAT	2 cut(s)	1666, 2564
BseDI	CCNNGG	3 cut(s)	123, 836, 2145
BseGI	GGATG	2 cut(s)	765, 1907
BseJI	GATNNNNATC	1 cut(s)	2541
BseLI	CCNNNNNNNGG	6 cut(s)	116, 1226, 1391, 1411, 1540, 1917
BseMII	CTCAG	7 cut(s)	983, 1056, 1130, 1414, 2015, 2202, 2513
BseNI	ACTGG	3 cut(s)	106, 110, 333
BseSI	GKGCMC	4 cut(s)	351, 438, 806, 2201
BseYI	CCCAGC	1 cut(s)	572
BsgI	GTGCAG	2 cut(s)	2191, 2431
Bsh1236I	CGCG	1 cut(s)	2275
Bsh1285I	CGRYCG	2 cut(s)	1293, 1564
BshFI	GGCC	6 cut(s)	860, 1384, 2199, 2265, 2293, 2534
BshNI	GGYRCC	1 cut(s)	801
BshTI	ACCGGT	1 cut(s)	966
BshVI	ATCGAT	2 cut(s)	1666, 2564
BsiEI	CGRYCG	2 cut(s)	1293, 1564
BsiHKAI	GWGCWC	4 cut(s)	351, 438, 1234, 2374
BsiSI	CCGG	6 cut(s)	125, 772, 967, 1289, 1475, 2154
BslFI	GGGAC	4 cut(s)	133, 659, 2291, 2410
BslI	CCNNNNNNNGG	6 cut(s)	116, 1226, 1391, 1411, 1540, 1917
BsmAI	GTCTC	7 cut(s)	941, 1593, 1842, 1883, 1901, 2043, 2235
BsmBI	CGTCTC	1 cut(s)	1901
BsmFI	GGGAC	4 cut(s)	133, 659, 2291, 2410
BsnI	GGCC	6 cut(s)	860, 1384, 2199, 2265, 2293, 2534
Bsp119I	TTCGAA	4 cut(s)	907, 1023, 1417, 2105
Bsp120I	GGGCCC	1 cut(s)	2197
Bsp1286I	GDGCHC	7 cut(s)	351, 438, 806, 1234, 1716, 2201, 2374
Bsp13I	TCCGGA	1 cut(s)	1474
Bsp1720I	GCTNAGC	1 cut(s)	1400
Bsp19I	CCATGG	1 cut(s)	2145
BspANI	GGCC	6 cut(s)	860, 1384, 2199, 2265, 2293, 2534
BspCNI	CTCAG	7 cut(s)	984, 1057, 1129, 1413, 2016, 2203, 2512
BspDI	ATCGAT	2 cut(s)	1666, 2564
BspEI	TCCGGA	1 cut(s)	1474
BspFNI	CGCG	1 cut(s)	2275
BspHI	TCATGA	1 cut(s)	1786
BspPI	GGATC	7 cut(s)	787, 2009, 2081, 2094, 2132, 2144, 2157
BspT104I	TTCGAA	4 cut(s)	907, 1023, 1417, 2105
BspT107I	GGYRCC	1 cut(s)	801
BsrFI	RCCGGY	2 cut(s)	966, 1288
BsrI	ACTGG	3 cut(s)	106, 110, 333
BssAI	RCCGGY	2 cut(s)	966, 1288
BssECI	CCNNGG	3 cut(s)	123, 836, 2145
BssNAI	GTATAC	1 cut(s)	1867
BssT1I	CCWWGG	2 cut(s)	836, 2145
Bst1107I	GTATAC	1 cut(s)	1867
Bst2UI	CCWGG	3 cut(s)	1227, 2118, 2295
Bst4CI	ACNGT	3 cut(s)	322, 1051, 1585
Bst6I	CTCTTC	3 cut(s)	732, 924, 1988
BstAPI	GCANNNNNTGC	1 cut(s)	1094
BstBAI	YACGTR	1 cut(s)	352
BstBI	TTCGAA	4 cut(s)	907, 1023, 1417, 2105
BstC8I	GCNNGC	1 cut(s)	717
BstDSI	CCRYGG	1 cut(s)	2145
BstF5I	GGATG	2 cut(s)	765, 1907
BstFNI	CGCG	1 cut(s)	2275
BstH2I	RGCGCY	1 cut(s)	1521
BstHHI	GCGC	2 cut(s)	1520, 2275
BstMAI	GTCTC	7 cut(s)	941, 1593, 1842, 1883, 1901, 2043, 2235
BstMCI	CGRYCG	2 cut(s)	1293, 1564
BstMWI	GCNNNNNNNGC	5 cut(s)	369, 1088, 1094, 1103, 2205
BstNI	CCWGG	3 cut(s)	1227, 2118, 2295
BstNSI	RCATGY	5 cut(s)	251, 379, 954, 1238, 1483
BstSCI	CCNGG	6 cut(s)	123, 771, 1225, 2116, 2152, 2293
BstSFI	CTRYAG	1 cut(s)	439
BstSLI	GKGCMC	4 cut(s)	351, 438, 806, 2201
BstUI	CGCG	1 cut(s)	2275
BstV2I	GAAGAC	2 cut(s)	1299, 1669
BstX2I	RGATCY	3 cut(s)	2086, 2137, 2149
BstXI	CCANNNNNNTGG	1 cut(s)	1380
BstYI	RGATCY	3 cut(s)	2086, 2137, 2149
BstZ17I	GTATAC	1 cut(s)	1867
Bsu15I	ATCGAT	2 cut(s)	1666, 2564
Bsu36I	CCTNAGG	1 cut(s)	2499
BsuRI	GGCC	6 cut(s)	860, 1384, 2199, 2265, 2293, 2534
BsuTUI	ATCGAT	2 cut(s)	1666, 2564
BtgI	CCRYGG	1 cut(s)	2145
BtrI	CACGTC	1 cut(s)	2125
BtsCI	GGATG	2 cut(s)	765, 1907
BtsI	GCAGTG	1 cut(s)	419
BtsIMutI	CAGTG	4 cut(s)	99, 419, 1581, 2208
Cac8I	GCNNGC	1 cut(s)	717
CaiI	CAGNNNCTG	1 cut(s)	1094
CciI	TCATGA	1 cut(s)	1786
CfoI	GCGC	2 cut(s)	1520, 2275
Cfr10I	RCCGGY	2 cut(s)	966, 1288
Cfr13I	GGNCC	4 cut(s)	120, 2197, 2198, 2264
ClaI	ATCGAT	2 cut(s)	1666, 2564
CseI	GACGC	1 cut(s)	1883
Csp6I	GTAC	2 cut(s)	251, 1052
CspAI	ACCGGT	1 cut(s)	966
CviQI	GTAC	2 cut(s)	251, 1052
DraIII	CACNNNGTG	2 cut(s)	640, 2338
EaeI	YGGCCR	2 cut(s)	1382, 2532
Eam1104I	CTCTTC	3 cut(s)	732, 924, 1988
EarI	CTCTTC	3 cut(s)	732, 924, 1988
EciI	GGCGGA	2 cut(s)	772, 1017
Eco130I	CCWWGG	2 cut(s)	836, 2145
Eco24I	GRGCYC	2 cut(s)	1716, 2201
Eco47I	GGWCC	1 cut(s)	120
Eco57I	CTGAAG	1 cut(s)	1641
Eco72I	CACGTG	1 cut(s)	352
Eco81I	CCTNAGG	1 cut(s)	2499
EcoO109I	RGGNCCY	4 cut(s)	120, 2197, 2198, 2264
EcoRII	CCWGG	3 cut(s)	1225, 2116, 2293
EcoT14I	CCWWGG	2 cut(s)	836, 2145
EcoT22I	ATGCAT	1 cut(s)	409
EcoT38I	GRGCYC	2 cut(s)	1716, 2201
ErhI	CCWWGG	2 cut(s)	836, 2145
Esp3I	CGTCTC	1 cut(s)	1901
FalI	AAGNNNNNCTT	2 cut(s)	75, 107
FaqI	GGGAC	4 cut(s)	133, 659, 2291, 2410
FauI	CCCGC	2 cut(s)	591, 2268
FbaI	TGATCA	1 cut(s)	2542
FblI	GTMKAC	1 cut(s)	1866
FokI	GGATG	2 cut(s)	772, 1894
FriOI	GRGCYC	2 cut(s)	1716, 2201
FspBI	CTAG	5 cut(s)	446, 926, 1142, 1449, 2574
GlaI	GCGC	2 cut(s)	1519, 2274
GsaI	CCCAGC	1 cut(s)	576
GsuI	CTGGAG	2 cut(s)	1035, 2358
HaeII	RGCGCY	1 cut(s)	1521
HaeIII	GGCC	6 cut(s)	860, 1384, 2199, 2265, 2293, 2534
HapII	CCGG	6 cut(s)	125, 772, 967, 1289, 1475, 2154
HgaI	GACGC	1 cut(s)	1883
HhaI	GCGC	2 cut(s)	1520, 2275
Hin6I	GCGC	2 cut(s)	1518, 2273
HinP1I	GCGC	2 cut(s)	1518, 2273
HincII	GTYRAC	2 cut(s)	138, 1836
HindII	GTYRAC	2 cut(s)	138, 1836
HpaII	CCGG	6 cut(s)	125, 772, 967, 1289, 1475, 2154
HphI	GGTGA	8 cut(s)	139, 226, 401, 779, 787, 1350, 2132, 2314
Hpy166II	GTNNAC	8 cut(s)	138, 217, 349, 436, 1431, 1836, 1867, 2322
Hpy188I	TCNGA	9 cut(s)	77, 382, 892, 1066, 1327, 1576, 1660, 2137, 2542
Hpy8I	GTNNAC	8 cut(s)	138, 217, 349, 436, 1431, 1836, 1867, 2322
Hpy99I	CGWCG	2 cut(s)	1298, 1394
HpyCH4III	ACNGT	3 cut(s)	322, 1051, 1585
HpyCH4IV	ACGT	3 cut(s)	351, 1263, 2124
HpyF10VI	GCNNNNNNNGC	5 cut(s)	369, 1088, 1094, 1103, 2205
HpySE526I	ACGT	3 cut(s)	351, 1263, 2124
HspAI	GCGC	2 cut(s)	1518, 2273
KflI	GGGWCCC	1 cut(s)	120
Kpn2I	TCCGGA	1 cut(s)	1474
Ksp22I	TGATCA	1 cut(s)	2542
LmnI	GCTCC	4 cut(s)	517, 1229, 2377, 2423
LweI	GCATC	9 cut(s)	256, 1115, 1166, 1187, 1256, 1633, 2260, 2556, 2570
MaeI	CTAG	5 cut(s)	446, 926, 1142, 1449, 2574
MaeII	ACGT	3 cut(s)	351, 1263, 2124
MaeIII	GTNAC	7 cut(s)	228, 535, 691, 865, 1356, 2120, 2485
MfeI	CAATTG	1 cut(s)	1374
MflI	RGATCY	3 cut(s)	2086, 2137, 2149
MhlI	GDGCHC	7 cut(s)	351, 438, 806, 1234, 1716, 2201, 2374
MlyI	GAGTC	2 cut(s)	460, 2351
MmeI	TCCRAC	4 cut(s)	893, 924, 1576, 2532
Mph1103I	ATGCAT	1 cut(s)	409
MroI	TCCGGA	1 cut(s)	1474
MroXI	GAANNNNTTC	2 cut(s)	1413, 2510
MseI	TTAA	7 cut(s)	48, 54, 95, 651, 1556, 1890, 2060
MslI	CAYNNNNRTG	2 cut(s)	27, 323
MspA1I	CMGCKG	4 cut(s)	586, 754, 1094, 1615
MspI	CCGG	6 cut(s)	125, 772, 967, 1289, 1475, 2154
MspR9I	CCNGG	6 cut(s)	125, 773, 1227, 2118, 2154, 2295
MunI	CAATTG	1 cut(s)	1374
MvaI	CCWGG	3 cut(s)	1227, 2118, 2295
MvnI	CGCG	1 cut(s)	2275
MwoI	GCNNNNNNNGC	5 cut(s)	369, 1088, 1094, 1103, 2205
NciI	CCSGG	3 cut(s)	125, 773, 2154
NcoI	CCATGG	1 cut(s)	2145
NmuCI	GTSAC	5 cut(s)	535, 865, 1356, 2120, 2485
NsiI	ATGCAT	1 cut(s)	409
NspI	RCATGY	5 cut(s)	251, 379, 954, 1238, 1483
NspV	TTCGAA	4 cut(s)	907, 1023, 1417, 2105
PagI	TCATGA	1 cut(s)	1786
PciI	ACATGT	2 cut(s)	1234, 1479
PdmI	GAANNNNTTC	2 cut(s)	1413, 2510
PflFI	GACNNNGTC	1 cut(s)	1875
PinAI	ACCGGT	1 cut(s)	966
PleI	GAGTC	2 cut(s)	459, 2350
PmaCI	CACGTG	1 cut(s)	352
PmlI	CACGTG	1 cut(s)	352
PpsI	GAGTC	2 cut(s)	459, 2350
Ppu21I	YACGTR	1 cut(s)	352
PpuMI	RGGWCCY	1 cut(s)	120
PscI	ACATGT	2 cut(s)	1234, 1479
PshBI	ATTAAT	1 cut(s)	54
Psp5II	RGGWCCY	1 cut(s)	120
Psp6I	CCWGG	3 cut(s)	1225, 2116, 2293
PspCI	CACGTG	1 cut(s)	352
PspFI	CCCAGC	1 cut(s)	572
PspGI	CCWGG	3 cut(s)	1225, 2116, 2293
PspOMI	GGGCCC	1 cut(s)	2197
PspPI	GGNCC	4 cut(s)	120, 2197, 2198, 2264
PspPPI	RGGWCCY	1 cut(s)	120
PstNI	CAGNNNCTG	1 cut(s)	1094
PsuI	RGATCY	3 cut(s)	2086, 2137, 2149
PsyI	GACNNNGTC	1 cut(s)	1875
PvuII	CAGCTG	2 cut(s)	1094, 1615
RsaI	GTAC	2 cut(s)	252, 1053
RsaNI	GTAC	2 cut(s)	251, 1052
RseI	CAYNNNNRTG	2 cut(s)	27, 323
SaqAI	TTAA	7 cut(s)	48, 54, 95, 651, 1556, 1890, 2060
Sau96I	GGNCC	4 cut(s)	120, 2197, 2198, 2264
SchI	GAGTC	2 cut(s)	460, 2351
ScrFI	CCNGG	6 cut(s)	125, 773, 1227, 2118, 2154, 2295
SduI	GDGCHC	7 cut(s)	351, 438, 806, 1234, 1716, 2201, 2374
SfaNI	GCATC	9 cut(s)	256, 1115, 1166, 1187, 1256, 1633, 2260, 2556, 2570
SfcI	CTRYAG	1 cut(s)	439
SfuI	TTCGAA	4 cut(s)	907, 1023, 1417, 2105
SinI	GGWCC	1 cut(s)	120
SmiMI	CAYNNNNRTG	2 cut(s)	27, 323
SmlI	CTYRAG	5 cut(s)	1209, 1257, 2222, 2237, 2300
SmoI	CTYRAG	5 cut(s)	1209, 1257, 2222, 2237, 2300
SspI	AATATT	1 cut(s)	1799
SspMI	CTAG	5 cut(s)	446, 926, 1142, 1449, 2574
StyD4I	CCNGG	6 cut(s)	123, 771, 1225, 2116, 2152, 2293
StyI	CCWWGG	2 cut(s)	836, 2145
TaaI	ACNGT	3 cut(s)	322, 1051, 1585
TaiI	ACGT	3 cut(s)	354, 1266, 2127
TatI	WGTACW	1 cut(s)	1051
TauI	GCSGC	4 cut(s)	757, 1288, 1387, 1967
Tru1I	TTAA	7 cut(s)	48, 54, 95, 651, 1556, 1890, 2060
Tru9I	TTAA	7 cut(s)	48, 54, 95, 651, 1556, 1890, 2060
TscAI	CASTG	4 cut(s)	106, 426, 1588, 2215
TseFI	GTSAC	5 cut(s)	535, 865, 1356, 2120, 2485
Tsp45I	GTSAC	5 cut(s)	535, 865, 1356, 2120, 2485
TspGWI	ACGGA	4 cut(s)	554, 1161, 1407, 1798
TspRI	CASTG	4 cut(s)	106, 426, 1588, 2215
Tth111I	GACNNNGTC	1 cut(s)	1875
VneI	GTGCAC	2 cut(s)	347, 434
VpaK11BI	GGWCC	1 cut(s)	120
VspI	ATTAAT	1 cut(s)	54
XapI	RAATTY	5 cut(s)	64, 542, 685, 1469, 2449
XbaI	TCTAGA	4 cut(s)	445, 925, 1141, 1448
XceI	RCATGY	5 cut(s)	251, 379, 954, 1238, 1483
XmiI	GTMKAC	1 cut(s)	1866
XmnI	GAANNNNTTC	2 cut(s)	1413, 2510
XspI	CTAG	5 cut(s)	446, 926, 1142, 1449, 2574
Zsp2I	ATGCAT	1 cut(s)	409

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.