pycom10g00610

Serine threonine-protein phosphatase 7 long form homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0001459
Physical Location & Seq
Reverse (-)
641218 .. 645040
3823 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g00610.2

Sequence Viewer

Length: 2232 bp
ATGGATGTTCACGAAGCACTGGACCTCTTGAATAAATTGGGGGAATGGAAGCTTGATGGAAGAGTTAAAGAGAGGATTAAAGACACTGGTTTTGATGGGTTACTTAAGCTCACTACTTTTCCAATTAACCATGATTTACCCCTATTATCTGTACTTGTAGAATCCTATGATGTTAGAGAGAGATGTTTCATTTTTAATAGCCATAAACTTTTTTTTGGTTTGGAAGATGTATTGTATATCACAGGGCTGCCTGTAGATGGTAATCCTGTCACCGGTATTGATTCAAGAGGTGATGAGCTTTGTATGAAATACTTGGGTTGCAATGGTATTGACCCAAGGAGTGCTGGTATCACTAGTTTAGAATGGCTGAGGGAGAATTTTGAGGTAGTTCCTGAGACTATTGATAAAGATAGTCCCGACATTGAACCTTATGTTCGAGCTTTTCTACTATATCTTCTAGGCTCTGTGGTTGTTCCTGATTACTCTCGGAAGTATGTCCATGTTATTTATCTTTCTCTCATGGAAAATCTTCAAAGCATAAAGGACTATGCATGGGGTGCTGCTTTGCTAGCTCATCTTCATTTTTCACTGGAGAACTTTAAGCAACTCTATTCACCTCGGCGAAAAAACATATTAATGGGACACACCTTTTCCCTTCTGGTTTTTGCAATGGAGCGTATTCCTAATCTGGTGCGGAGATTTTTGTCCAATGGAGTCAATCGGGTGGATGATTATCTTCCAACAAGTTTTCCTTTGTTAGCAGGATGGACCAAACTACTATGCGACCCCTCCAATACTCATGAAGAAACCACTAGACAGGAATATCTTCAAATGCTGGATGAGCTAATAGAGGATGATGTAAGTGTTTTTGCATTTGGCTTTTGTAAGTTGATTGTTTGGCAGCCATACAAAAGGCTTCCCTGTAATTTTCTTCCCGAGTATTGTGCCGGGCAGGAGAAAATGGGCATGTCTAGGACAATCCTCCTTTGCTATGAGAAGGCTGTGTATCATCGGCCCGATTTCAGTCCAAAGCAATTTGGCATTCAGAAAGTGAATACCAGTATGTTGCGCCCCCTTGTTGAGTTGGAATTATGTCCTAAGAGGGGTCGCAAAGGAATAAATTGGGGAACGTATAAAAATTATGGATGTTACAAGGAGGAGTGGGAAAATAGGGCCAGCTGCATCATAACAGAATCTGTTGAAGAGGGTCCTAACTCCCCTTCGATTGAAGCGCTTACTACTTCGTCTTCTCTGGCCCCTGATGACCCTAATCCACTGTCTGCTAAGAGGAAGAGCGTTGACCATGTTGGCAACACACTCAAGGTTAAGACACCGAAGACATCAGGCCCCCTTGTAGATACTTATGGGTCCACTAACTCCTTGGAATTAGGGAATTCTCTGCACGCAGACTTATATTTTGGACTTACTGATGCCGCTCCTATGGATGCGACTCCGCCTCCGTCGATGGGGATATTCTCAGTGACATCTGATCGGGAGGATCCTGCAACCATTCATCCCTCAAATGCAATTTCAAGCTTGCTGACTGACCTTAATGATTCAGGCTTATTTCCTCCGGCCAGATCACCCAAGTTGTTGGATGAGTTGAATTCTTTACAGGACGATGTTAATGACCTTCTTCAGGCATCTATTACCCATCCTTCTGATGTTCGTCCCACAGCTATGCCAGCGGCCGTGGAGTCTGCCAAGGAGACTATCCGAGGAATCCTATGTCGAGGTTTCGATGCCTTAGCAAACTCTGAAACGCAAGAAGCTTTTCTTGCTTCTTCAGATGTTCTATTGTCATCCGGTGTCTTCCCATCTGATGATTTGAAGACTAGGTTGACAGCCTTTAGGAATGAGCTCCCGCGCAATGCTACTGCCTTTCTACAAGCACATGCAGATGTTGATACAGCTACAGAGTTTTCTTCTTTACAGGAATTCTTGGTCTCGCAAGCCGTCAAACTTCCTGCCCTGCAAGAACGCTTACACGTGGCTTCTGAGAAGATAGCACATCTTCGAGCAATATTAGATGCTGCCCTTGAGAAGAAAACGAAGATGGATGAGGTTCTCGCTGCCTTTGTTGAGAAGACTAAAACTGCCAAGCAGAAATTGGCCTCCATGCATCCTCAGGCAGCTGAAATCGAGTCAAAGAAGCGGGCATCCGAGAAGTTGATGGAGCAATTCAGATCCTCTTGGGTTGACCTTAGGACTAGTTTGTTCAAATTTTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PMD

Protein Analysis

744

Amino Acids

83.25

Weight (kDa)

5.48

Isoelectric Point (pI)

41.86

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 1436
AccII CGCG 1 cut(s) 1867
AciI CCGC 6 cut(s) 694, 1434, 1454, 1688, 1865, 2155
AclWI GGATC 3 cut(s) 1493, 1506, 2181
AcoI YGGCCR 2 cut(s) 1575, 1689
AcsI RAATTY 5 cut(s) 376, 1393, 1606, 1937, 2222
AcuI CTGAAG 2 cut(s) 1622, 1770
AcvI CACGTG 1 cut(s) 1990
AfaI GTAC 1 cut(s) 153
AfeI AGCGCT 1 cut(s) 1233
AfiI CCNNNNNNNGG 5 cut(s) 257, 272, 1103, 1466, 1639
AflII CTTAAG 1 cut(s) 104
AflIII ACRYGT 1 cut(s) 1987
AgeI ACCGGT 1 cut(s) 272
AhlI ACTAGT 2 cut(s) 353, 2210
Alw21I GWGCWC 1 cut(s) 1863
Alw26I GTCTC 3 cut(s) 389, 1703, 1951
AlwI GGATC 3 cut(s) 1493, 1506, 2181
AlwNI CAGNNNCTG 1 cut(s) 1196
Ama87I CYCGRG 1 cut(s) 935
Aor51HI AGCGCT 1 cut(s) 1233
AoxI GGCC 7 cut(s) 1013, 1173, 1254, 1345, 1575, 1689, 2112
ApeKI GCWGC 7 cut(s) 247, 560, 901, 1179, 2033, 2072, 2132
ApoI RAATTY 5 cut(s) 376, 1393, 1606, 1937, 2222
ArsI GACNNNNNNTTYG 4 cut(s) 74, 106, 1400, 1432
AseI ATTAAT 1 cut(s) 635
AsiGI ACCGGT 1 cut(s) 272
Asp700I GAANNNNTTC 3 cut(s) 528, 825, 1773
AspLEI GCGC 3 cut(s) 1071, 1234, 1869
AspS9I GGNCC 8 cut(s) 22, 768, 1014, 1173, 1208, 1255, 1346, 1368
AsuC2I CCSGG 1 cut(s) 949
AsuHPI GGTGA 4 cut(s) 262, 302, 606, 1575
AsuNHI GCTAGC 1 cut(s) 568
AvaI CYCGRG 1 cut(s) 935
AvaII GGWCC 4 cut(s) 22, 768, 1208, 1368
AxyI CCTNAGG 2 cut(s) 2127, 2204
BamHI GGATCC 1 cut(s) 1498
BanII GRGCYC 1 cut(s) 1863
BbrPI CACGTG 1 cut(s) 1990
BbsI GAAGAC 5 cut(s) 1239, 1343, 1804, 1838, 2093
Bbv12I GWGCWC 1 cut(s) 1863
BbvCI CCTCAGC 1 cut(s) 368
BbvI GCAGC 7 cut(s) 234, 547, 913, 1166, 2020, 2059, 2144
BccI CCATC 9 cut(s) 50, 89, 251, 759, 1459, 1662, 1825, 2050, 2167
BceAI ACGGC 2 cut(s) 1676, 1940
BcgI CGANNNNNNTGC 2 cut(s) 926, 960
BcnI CCSGG 1 cut(s) 949
BcoDI GTCTC 3 cut(s) 389, 1703, 1951
BcuI ACTAGT 2 cut(s) 353, 2210
BfaI CTAG 7 cut(s) 354, 458, 569, 813, 972, 1836, 2211
BfmI CTRYAG 2 cut(s) 252, 1914
BfoI RGCGCY 1 cut(s) 1235
BfrI CTTAAG 1 cut(s) 104
BisI GCNGC 9 cut(s) 248, 561, 902, 1180, 1434, 1689, 2034, 2073, 2133
BlsI GCNGC 9 cut(s) 249, 562, 903, 1181, 1435, 1690, 2035, 2074, 2134
Bme1390I CCNGG 1 cut(s) 949
Bme18I GGWCC 4 cut(s) 22, 768, 1208, 1368
BmeT110I CYCGRG 1 cut(s) 935
BmgT120I GGNCC 8 cut(s) 22, 768, 1014, 1173, 1208, 1255, 1346, 1368
BmiI GGNNCC 5 cut(s) 1209, 1257, 1348, 1369, 1500
BmrFI CCNGG 1 cut(s) 949
BmsI GCATC 8 cut(s) 1191, 1420, 1435, 1652, 1732, 2020, 2131, 2168
BmtI GCTAGC 1 cut(s) 572
BpiI GAAGAC 5 cut(s) 1239, 1343, 1804, 1838, 2093
BpmI CTGGAG 1 cut(s) 611
Bpu10I CCTNAGC 2 cut(s) 368, 1747
BpuEI CTTGAG 2 cut(s) 1304, 2060
BpuMI CCSGG 1 cut(s) 949
BsaAI YACGTR 1 cut(s) 1990
BsaBI GATNNNNATC 2 cut(s) 261, 732
BsaI GGTCTC 1 cut(s) 1951
BsaJI CCNNGG 6 cut(s) 335, 617, 1380, 1692, 1704, 1717
BsaWI WCCGGW 2 cut(s) 272, 1805
BsaXI ACNNNNNCTCC 4 cut(s) 331, 361, 1441, 1471
Bsc4I CCNNNNNNNGG 5 cut(s) 257, 272, 1103, 1466, 1639
Bse118I RCCGGY 1 cut(s) 272
Bse1I ACTGG 4 cut(s) 24, 91, 594, 1059
Bse21I CCTNAGG 2 cut(s) 2127, 2204
Bse3DI GCAATG 3 cut(s) 328, 675, 1876
Bse8I GATNNNNATC 2 cut(s) 261, 732
BseDI CCNNGG 6 cut(s) 335, 617, 1380, 1692, 1704, 1717
BseJI GATNNNNATC 2 cut(s) 261, 732
BseLI CCNNNNNNNGG 5 cut(s) 257, 272, 1103, 1466, 1639
BseMI GCAATG 3 cut(s) 328, 675, 1876
BseMII CTCAG 5 cut(s) 359, 384, 1491, 1989, 2141
BseNI ACTGG 4 cut(s) 24, 91, 594, 1059
BseRI GAGGAG 1 cut(s) 1172
BseX3I CGGCCG 1 cut(s) 1689
BseXI GCAGC 7 cut(s) 234, 547, 913, 1166, 2020, 2059, 2144
BsgI GTGCAG 1 cut(s) 1385
Bsh1236I CGCG 1 cut(s) 1867
Bsh1285I CGRYCG 1 cut(s) 1692
BshFI GGCC 7 cut(s) 1015, 1175, 1256, 1347, 1577, 1691, 2114
BshTI ACCGGT 1 cut(s) 272
BsiEI CGRYCG 1 cut(s) 1692
BsiHKAI GWGCWC 1 cut(s) 1863
BsiHKCI CYCGRG 1 cut(s) 935
BsiSI CCGG 4 cut(s) 273, 948, 1574, 1806
BslFI GGGAC 3 cut(s) 399, 654, 1656
BslI CCNNNNNNNGG 5 cut(s) 257, 272, 1103, 1466, 1639
BsmAI GTCTC 3 cut(s) 389, 1703, 1951
BsmFI GGGAC 3 cut(s) 399, 654, 1656
BsmI GAATGC 1 cut(s) 1041
BsnI GGCC 7 cut(s) 1015, 1175, 1256, 1347, 1577, 1691, 2114
Bso31I GGTCTC 1 cut(s) 1951
BsoBI CYCGRG 1 cut(s) 935
Bsp1286I GDGCHC 1 cut(s) 1863
Bsp143I GATC 4 cut(s) 1489, 1498, 1580, 2186
BspACI CCGC 6 cut(s) 694, 1434, 1454, 1688, 1865, 2155
BspANI GGCC 7 cut(s) 1015, 1175, 1256, 1347, 1577, 1691, 2114
BspCNI CTCAG 5 cut(s) 360, 385, 1490, 1990, 2140
BspFNI CGCG 1 cut(s) 1867
BspHI TCATGA 1 cut(s) 799
BspLI GGNNCC 5 cut(s) 1209, 1257, 1348, 1369, 1500
BspOI GCTAGC 1 cut(s) 572
BspPI GGATC 3 cut(s) 1493, 1506, 2181
BspQI GCTCTTC 1 cut(s) 1286
BspTI CTTAAG 1 cut(s) 104
BspTNI GGTCTC 1 cut(s) 1951
BsrBI CCGCTC 1 cut(s) 1436
BsrDI GCAATG 3 cut(s) 328, 675, 1876
BsrFI RCCGGY 1 cut(s) 272
BsrI ACTGG 4 cut(s) 24, 91, 594, 1059
BssAI RCCGGY 1 cut(s) 272
BssECI CCNNGG 6 cut(s) 335, 617, 1380, 1692, 1704, 1717
BssMI GATC 4 cut(s) 1489, 1498, 1580, 2186
BssT1I CCWWGG 3 cut(s) 335, 1380, 1704
Bst4CI ACNGT 1 cut(s) 1278
Bst6I CTCTTC 3 cut(s) 55, 1197, 1286
BstAFI CTTAAG 1 cut(s) 104
BstAPI GCANNNNNTGC 1 cut(s) 557
BstBAI YACGTR 1 cut(s) 1990
BstC8I GCNNGC 7 cut(s) 570, 1177, 1404, 1538, 1686, 1953, 2157
BstDEI CTNAG 9 cut(s) 368, 393, 1098, 1284, 1477, 1747, 1998, 2127, 2204
BstDSI CCRYGG 1 cut(s) 1692
BstENI CCTNNNNNAGG 1 cut(s) 1637
BstFNI CGCG 1 cut(s) 1867
BstH2I RGCGCY 1 cut(s) 1235
BstHHI GCGC 3 cut(s) 1071, 1234, 1869
BstKTI GATC 4 cut(s) 1492, 1501, 1583, 2189
BstMAI GTCTC 3 cut(s) 389, 1703, 1951
BstMBI GATC 4 cut(s) 1489, 1498, 1580, 2186
BstMCI CGRYCG 1 cut(s) 1692
BstMWI GCNNNNNNNGC 5 cut(s) 557, 569, 841, 1685, 1778
BstNSI RCATGY 2 cut(s) 970, 1898
BstSCI CCNGG 1 cut(s) 947
BstSFI CTRYAG 2 cut(s) 252, 1914
BstUI CGCG 1 cut(s) 1867
BstV1I GCAGC 7 cut(s) 234, 547, 913, 1166, 2020, 2059, 2144
BstV2I GAAGAC 5 cut(s) 1239, 1343, 1804, 1838, 2093
BstX2I RGATCY 2 cut(s) 1498, 2186
BstXI CCANNNNNNTGG 1 cut(s) 1594
BstYI RGATCY 2 cut(s) 1498, 2186
BstZI CGGCCG 1 cut(s) 1689
Bsu36I CCTNAGG 2 cut(s) 2127, 2204
BsuRI GGCC 7 cut(s) 1015, 1175, 1256, 1347, 1577, 1691, 2114
BtgI CCRYGG 1 cut(s) 1692
BtsIMutI CAGTG 5 cut(s) 17, 84, 587, 1274, 1485
Cac8I GCNNGC 7 cut(s) 570, 1177, 1404, 1538, 1686, 1953, 2157
CaiI CAGNNNCTG 1 cut(s) 1196
CciI TCATGA 1 cut(s) 799
CfoI GCGC 3 cut(s) 1071, 1234, 1869
Cfr10I RCCGGY 1 cut(s) 272
Cfr13I GGNCC 8 cut(s) 22, 768, 1014, 1173, 1208, 1255, 1346, 1368
Csp6I GTAC 1 cut(s) 152
CspAI ACCGGT 1 cut(s) 272
CviAII CATG 9 cut(s) 131, 500, 520, 552, 800, 967, 1304, 1895, 2119
CviQI GTAC 1 cut(s) 152
DdeI CTNAG 9 cut(s) 368, 393, 1098, 1284, 1477, 1747, 1998, 2127, 2204
DpnI GATC 4 cut(s) 1491, 1500, 1582, 2188
DpnII GATC 4 cut(s) 1489, 1498, 1580, 2186
EaeI YGGCCR 2 cut(s) 1575, 1689
EagI CGGCCG 1 cut(s) 1689
Eam1104I CTCTTC 3 cut(s) 55, 1197, 1286
EarI CTCTTC 3 cut(s) 55, 1197, 1286
EciI GGCGGA 1 cut(s) 1443
Ecl136II GAGCTC 1 cut(s) 1861
EclXI CGGCCG 1 cut(s) 1689
Eco130I CCWWGG 3 cut(s) 335, 1380, 1704
Eco24I GRGCYC 1 cut(s) 1863
Eco31I GGTCTC 1 cut(s) 1951
Eco47I GGWCC 4 cut(s) 22, 768, 1208, 1368
Eco47III AGCGCT 1 cut(s) 1233
Eco52I CGGCCG 1 cut(s) 1689
Eco53kI GAGCTC 1 cut(s) 1861
Eco57I CTGAAG 2 cut(s) 1622, 1770
Eco72I CACGTG 1 cut(s) 1990
Eco81I CCTNAGG 2 cut(s) 2127, 2204
Eco88I CYCGRG 1 cut(s) 935
EcoICRI GAGCTC 1 cut(s) 1861
EcoNI CCTNNNNNAGG 1 cut(s) 1637
EcoO109I RGGNCCY 2 cut(s) 1208, 1346
EcoRI GAATTC 3 cut(s) 1393, 1606, 1937
EcoT14I CCWWGG 3 cut(s) 335, 1380, 1704
EcoT22I ATGCAT 2 cut(s) 553, 2124
EcoT38I GRGCYC 1 cut(s) 1863
ErhI CCWWGG 3 cut(s) 335, 1380, 1704
FaeI CATG 9 cut(s) 134, 503, 523, 555, 803, 970, 1307, 1898, 2122
FalI AAGNNNNNCTT 4 cut(s) 736, 768, 1761, 1793
FaqI GGGAC 3 cut(s) 399, 654, 1656
FatI CATG 9 cut(s) 130, 499, 519, 551, 799, 966, 1303, 1894, 2118
FauI CCCGC 2 cut(s) 1872, 2148
Fnu4HI GCNGC 9 cut(s) 248, 561, 902, 1180, 1434, 1689, 2034, 2073, 2133
FriOI GRGCYC 1 cut(s) 1863
Fsp4HI GCNGC 9 cut(s) 248, 561, 902, 1180, 1434, 1689, 2034, 2073, 2133
FspBI CTAG 7 cut(s) 354, 458, 569, 813, 972, 1836, 2211
GlaI GCGC 3 cut(s) 1070, 1233, 1868
GluI GCNGC 9 cut(s) 248, 561, 902, 1180, 1434, 1689, 2034, 2073, 2133
GsuI CTGGAG 1 cut(s) 611
HaeII RGCGCY 1 cut(s) 1235
HaeIII GGCC 7 cut(s) 1015, 1175, 1256, 1347, 1577, 1691, 2114
HapII CCGG 4 cut(s) 273, 948, 1574, 1806
HhaI GCGC 3 cut(s) 1071, 1234, 1869
Hin1II CATG 9 cut(s) 134, 503, 523, 555, 803, 970, 1307, 1898, 2122
Hin6I GCGC 3 cut(s) 1069, 1232, 1867
HinP1I GCGC 3 cut(s) 1069, 1232, 1867
HincII GTYRAC 3 cut(s) 1300, 1842, 2200
HindII GTYRAC 3 cut(s) 1300, 1842, 2200
HindIII AAGCTT 3 cut(s) 50, 1534, 1770
HinfI GANTC 9 cut(s) 161, 281, 714, 1193, 1450, 1556, 1697, 1722, 2144
HpaII CCGG 4 cut(s) 273, 948, 1574, 1806
HphI GGTGA 4 cut(s) 262, 302, 606, 1575
Hpy166II GTNNAC 5 cut(s) 10, 1300, 1371, 1842, 2200
Hpy188III TCNNGA 9 cut(s) 11, 28, 285, 392, 416, 476, 800, 935, 1493
Hpy8I GTNNAC 5 cut(s) 10, 1300, 1371, 1842, 2200
Hpy99I CGWCG 1 cut(s) 1465
HpyAV CCTTC 5 cut(s) 665, 991, 1230, 1643, 1668
HpyCH4III ACNGT 1 cut(s) 1278
HpyCH4IV ACGT 2 cut(s) 1130, 1989
HpyF10VI GCNNNNNNNGC 5 cut(s) 557, 569, 841, 1685, 1778
HpyF3I CTNAG 9 cut(s) 368, 393, 1098, 1284, 1477, 1747, 1998, 2127, 2204
HpySE526I ACGT 2 cut(s) 1130, 1989
Hsp92II CATG 9 cut(s) 134, 503, 523, 555, 803, 970, 1307, 1898, 2122
HspAI GCGC 3 cut(s) 1069, 1232, 1867
Kzo9I GATC 4 cut(s) 1489, 1498, 1580, 2186
LguI GCTCTTC 1 cut(s) 1286
LmnI GCTCC 4 cut(s) 673, 1441, 1866, 2176
Lsp1109I GCAGC 7 cut(s) 234, 547, 913, 1166, 2020, 2059, 2144
LweI GCATC 8 cut(s) 1191, 1420, 1435, 1652, 1732, 2020, 2131, 2168
MaeI CTAG 7 cut(s) 354, 458, 569, 813, 972, 1836, 2211
MaeII ACGT 2 cut(s) 1130, 1989
MaeIII GTNAC 4 cut(s) 99, 268, 1148, 1480
MalI GATC 4 cut(s) 1491, 1500, 1582, 2188
MbiI CCGCTC 1 cut(s) 1436
MboI GATC 4 cut(s) 1489, 1498, 1580, 2186
MflI RGATCY 2 cut(s) 1498, 2186
MhlI GDGCHC 1 cut(s) 1863
MlyI GAGTC 4 cut(s) 723, 1444, 1706, 2153
MmeI TCCRAC 3 cut(s) 764, 1065, 1575
Mph1103I ATGCAT 2 cut(s) 553, 2124
MroXI GAANNNNTTC 3 cut(s) 528, 825, 1773
MslI CAYNNNNRTG 3 cut(s) 635, 1679, 1899
MspA1I CMGCKG 3 cut(s) 1179, 1688, 2135
MspCI CTTAAG 1 cut(s) 104
MspI CCGG 4 cut(s) 273, 948, 1574, 1806
MspR9I CCNGG 1 cut(s) 949
Mva1269I GAATGC 1 cut(s) 1041
MvnI CGCG 1 cut(s) 1867
MwoI GCNNNNNNNGC 5 cut(s) 557, 569, 841, 1685, 1778
NciI CCSGG 1 cut(s) 949
NdeII GATC 4 cut(s) 1489, 1498, 1580, 2186
NheI GCTAGC 1 cut(s) 568
NlaIII CATG 9 cut(s) 134, 503, 523, 555, 803, 970, 1307, 1898, 2122
NlaIV GGNNCC 5 cut(s) 1209, 1257, 1348, 1369, 1500
NmeAIII GCCGAG 1 cut(s) 598
NmuCI GTSAC 2 cut(s) 268, 1480
NsiI ATGCAT 2 cut(s) 553, 2124
NspI RCATGY 2 cut(s) 970, 1898
PagI TCATGA 1 cut(s) 799
PciSI GCTCTTC 1 cut(s) 1286
PctI GAATGC 1 cut(s) 1041
PdmI GAANNNNTTC 3 cut(s) 528, 825, 1773
PfeI GAWTC 5 cut(s) 161, 281, 1193, 1556, 1722
PinAI ACCGGT 1 cut(s) 272
PkrI GCNGC 9 cut(s) 249, 562, 903, 1181, 1435, 1690, 2035, 2074, 2134
PleI GAGTC 4 cut(s) 722, 1444, 1705, 2152
PmaCI CACGTG 1 cut(s) 1990
PmlI CACGTG 1 cut(s) 1990
PpsI GAGTC 4 cut(s) 722, 1444, 1705, 2152
Ppu21I YACGTR 1 cut(s) 1990
PpuMI RGGWCCY 1 cut(s) 1208
PshBI ATTAAT 1 cut(s) 635
Psp124BI GAGCTC 1 cut(s) 1863
Psp5II RGGWCCY 1 cut(s) 1208
PspCI CACGTG 1 cut(s) 1990
PspN4I GGNNCC 5 cut(s) 1209, 1257, 1348, 1369, 1500
PspPI GGNCC 8 cut(s) 22, 768, 1014, 1173, 1208, 1255, 1346, 1368
PspPPI RGGWCCY 1 cut(s) 1208
PstNI CAGNNNCTG 1 cut(s) 1196
PsuI RGATCY 2 cut(s) 1498, 2186
PvuII CAGCTG 2 cut(s) 1179, 2135
RsaI GTAC 1 cut(s) 153
RsaNI GTAC 1 cut(s) 152
RseI CAYNNNNRTG 3 cut(s) 635, 1679, 1899
SacI GAGCTC 1 cut(s) 1863
SapI GCTCTTC 1 cut(s) 1286
SatI GCNGC 9 cut(s) 248, 561, 902, 1180, 1434, 1689, 2034, 2073, 2133
Sau3AI GATC 4 cut(s) 1489, 1498, 1580, 2186
Sau96I GGNCC 8 cut(s) 22, 768, 1014, 1173, 1208, 1255, 1346, 1368
SchI GAGTC 4 cut(s) 723, 1444, 1706, 2153
ScrFI CCNGG 1 cut(s) 949
SduI GDGCHC 1 cut(s) 1863
SfaNI GCATC 8 cut(s) 1191, 1420, 1435, 1652, 1732, 2020, 2131, 2168
SfcI CTRYAG 2 cut(s) 252, 1914
SinI GGWCC 4 cut(s) 22, 768, 1208, 1368
SmiMI CAYNNNNRTG 3 cut(s) 635, 1679, 1899
SmlI CTYRAG 3 cut(s) 104, 1319, 2039
SmoI CTYRAG 3 cut(s) 104, 1319, 2039
SpeI ACTAGT 2 cut(s) 353, 2210
SsiI CCGC 6 cut(s) 694, 1434, 1454, 1688, 1865, 2155
SspI AATATT 1 cut(s) 2025
SspMI CTAG 7 cut(s) 354, 458, 569, 813, 972, 1836, 2211
SstI GAGCTC 1 cut(s) 1863
StyD4I CCNGG 1 cut(s) 947
StyI CCWWGG 3 cut(s) 335, 1380, 1704
TaaI ACNGT 1 cut(s) 1278
TaiI ACGT 2 cut(s) 1133, 1992
TaqI TCGA 7 cut(s) 436, 1223, 1463, 1732, 1740, 2017, 2142
TatI WGTACW 1 cut(s) 151
TauI GCSGC 2 cut(s) 1436, 1691
TfiI GAWTC 5 cut(s) 161, 281, 1193, 1556, 1722
TscAI CASTG 5 cut(s) 24, 91, 594, 1281, 1485
TseFI GTSAC 2 cut(s) 268, 1480
TseI GCWGC 7 cut(s) 247, 560, 901, 1179, 2033, 2072, 2132
Tsp45I GTSAC 2 cut(s) 268, 1480
TspDTI ATGAA 5 cut(s) 178, 320, 569, 816, 1502
TspGWI ACGGA 1 cut(s) 1449
TspRI CASTG 5 cut(s) 24, 91, 594, 1281, 1485
Vha464I CTTAAG 1 cut(s) 104
VpaK11BI GGWCC 4 cut(s) 22, 768, 1208, 1368
VspI ATTAAT 1 cut(s) 635
XagI CCTNNNNNAGG 1 cut(s) 1637
XapI RAATTY 5 cut(s) 376, 1393, 1606, 1937, 2222
XceI RCATGY 2 cut(s) 970, 1898
XcmI CCANNNNNNNNNTGG 2 cut(s) 1378, 2107
XmnI GAANNNNTTC 3 cut(s) 528, 825, 1773
XspI CTAG 7 cut(s) 354, 458, 569, 813, 972, 1836, 2211
Zsp2I ATGCAT 2 cut(s) 553, 2124
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.