MD14G1065100.v1.1

Zinc finger A20 and AN1 domain-containing stress-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0000730
Physical Location & Seq
Reverse (-)
6910483 .. 6911187
705 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1065100.v1.1.491

Sequence Viewer

Length: 450 bp
ATGTATTCATTATGTTGGGCATTTGGTTATTTGTGTTACAAGGACACGCTCTTAAAGCAGGACCAAGCTAATTTGGCTGCATCATCCATTGACAGTATTGTGAATGGCGTCGATGGCAGCAGCAACATCGTCATTGACCCCGTTGTTGCAAGTGTTGTCGATGTGCAAGCTGTTCAAGTGGGGACCAGTATTGTTTTAACAGAACCATCCAGTGACTCATCCTCAAGCATGAAAATTGAGGTGAAAGAAAAAAAGGGACCAAGCAAATGCACTACTTGCCGAAAGCGCGTTGGTTTAACTGGGTTCAATTGCAAATGTGGAAACACCTTCTGTGCAAGCCATCGTTATTCCGACAAACATGATTGTCCTTTTGATTATAAGACTGCTGGTCAGGATGCTATTGCGAAAGCCAATCCTATTGTGAAGGCAGACAAACTTGATAAAATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-A20 zf-AN1

Protein Analysis

150

Amino Acids

16.02

Weight (kDa)

8.08

Isoelectric Point (pI)

30.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-AN1 PF01428 90 - 127 3.4e-10 AN1-like Zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 378
AccII CGCG 1 cut(s) 288
AcyI GRCGYC 1 cut(s) 108
AgsI TTSAA 2 cut(s) 176, 307
AhdI GACNNNNNGTC 1 cut(s) 387
AluBI AGCT 2 cut(s) 68, 170
AluI AGCT 2 cut(s) 68, 170
ApeKI GCWGC 3 cut(s) 77, 117, 120
AspLEI GCGC 1 cut(s) 288
AspS9I GGNCC 3 cut(s) 61, 183, 257
AsuHPI GGTGA 1 cut(s) 253
AvaII GGWCC 3 cut(s) 61, 183, 257
BbvI GCAGC 3 cut(s) 64, 129, 132
BccI CCATC 3 cut(s) 107, 214, 348
BcgI CGANNNNNNTGC 2 cut(s) 109, 143
BfaI CTAG 1 cut(s) 448
BisI GCNGC 3 cut(s) 78, 118, 121
BlsI GCNGC 3 cut(s) 79, 119, 122
Bme18I GGWCC 3 cut(s) 61, 183, 257
BmeRI GACNNNNNGTC 1 cut(s) 387
BmgT120I GGNCC 3 cut(s) 61, 183, 257
BmiI GGNNCC 2 cut(s) 184, 258
BmrI ACTGGG 1 cut(s) 309
BmsI GCATC 2 cut(s) 89, 385
BmuI ACTGGG 1 cut(s) 309
BpuEI CTTGAG 1 cut(s) 208
BsaHI GRCGYC 1 cut(s) 108
Bse1I ACTGG 3 cut(s) 186, 210, 304
BseGI GGATG 4 cut(s) 83, 206, 218, 400
BseNI ACTGG 3 cut(s) 186, 210, 304
BseXI GCAGC 3 cut(s) 64, 129, 132
Bsh1236I CGCG 1 cut(s) 288
BslFI GGGAC 2 cut(s) 196, 270
BsmFI GGGAC 2 cut(s) 196, 270
BspFNI CGCG 1 cut(s) 288
BspLI GGNNCC 2 cut(s) 184, 258
BsrI ACTGG 3 cut(s) 186, 210, 304
BssNI GRCGYC 1 cut(s) 108
Bst4CI ACNGT 1 cut(s) 95
BstACI GRCGYC 1 cut(s) 108
BstAPI GCANNNNNTGC 1 cut(s) 276
BstC8I GCNNGC 2 cut(s) 168, 337
BstF5I GGATG 4 cut(s) 83, 206, 218, 400
BstFNI CGCG 1 cut(s) 288
BstHHI GCGC 1 cut(s) 288
BstMWI GCNNNNNNNGC 5 cut(s) 55, 74, 114, 276, 285
BstUI CGCG 1 cut(s) 288
BstV1I GCAGC 3 cut(s) 64, 129, 132
BtsCI GGATG 4 cut(s) 83, 206, 218, 400
BtsIMutI CAGTG 1 cut(s) 217
Cac8I GCNNGC 2 cut(s) 168, 337
CfoI GCGC 1 cut(s) 288
Cfr13I GGNCC 3 cut(s) 61, 183, 257
CseI GACGC 1 cut(s) 97
CviAII CATG 2 cut(s) 229, 359
CviJI RGCY 5 cut(s) 68, 77, 170, 339, 410
CviKI_1 RGCY 5 cut(s) 68, 77, 170, 339, 410
DriI GACNNNNNGTC 1 cut(s) 387
Eam1105I GACNNNNNGTC 1 cut(s) 387
Eco47I GGWCC 3 cut(s) 61, 183, 257
FaeI CATG 2 cut(s) 232, 362
FaiI YATR 4 cut(s) 13, 230, 360, 378
FaqI GGGAC 2 cut(s) 196, 270
FatI CATG 2 cut(s) 228, 358
Fnu4HI GCNGC 3 cut(s) 78, 118, 121
FokI GGATG 4 cut(s) 70, 193, 205, 407
Fsp4HI GCNGC 3 cut(s) 78, 118, 121
FspBI CTAG 1 cut(s) 448
GlaI GCGC 1 cut(s) 287
GluI GCNGC 3 cut(s) 78, 118, 121
HgaI GACGC 1 cut(s) 97
HhaI GCGC 1 cut(s) 288
Hin1I GRCGYC 1 cut(s) 108
Hin1II CATG 2 cut(s) 232, 362
Hin6I GCGC 1 cut(s) 286
HinP1I GCGC 1 cut(s) 286
HinfI GANTC 1 cut(s) 215
HphI GGTGA 1 cut(s) 253
Hpy188I TCNGA 1 cut(s) 352
Hpy188III TCNNGA 1 cut(s) 392
Hpy99I CGWCG 1 cut(s) 113
HpyAV CCTTC 2 cut(s) 337, 418
HpyCH4III ACNGT 1 cut(s) 95
HpyCH4V TGCA 6 cut(s) 80, 149, 166, 270, 312, 335
HpyF10VI GCNNNNNNNGC 5 cut(s) 55, 74, 114, 276, 285
Hsp92I GRCGYC 1 cut(s) 108
Hsp92II CATG 2 cut(s) 232, 362
HspAI GCGC 1 cut(s) 286
LpnPI CCDG 6 cut(s) 44, 199, 223, 285, 372, 377
Lsp1109I GCAGC 3 cut(s) 64, 129, 132
LweI GCATC 2 cut(s) 89, 385
MaeI CTAG 1 cut(s) 448
MaeIII GTNAC 2 cut(s) 35, 212
MfeI CAATTG 1 cut(s) 307
MluCI AATT 3 cut(s) 70, 234, 307
MlyI GAGTC 1 cut(s) 209
MmeI TCCRAC 1 cut(s) 375
MnlI CCTC 2 cut(s) 232, 232
MseI TTAA 3 cut(s) 53, 197, 296
MunI CAATTG 1 cut(s) 307
MvnI CGCG 1 cut(s) 288
MwoI GCNNNNNNNGC 5 cut(s) 55, 74, 114, 276, 285
NlaIII CATG 2 cut(s) 232, 362
NlaIV GGNNCC 2 cut(s) 184, 258
NmuCI GTSAC 1 cut(s) 212
PkrI GCNGC 3 cut(s) 79, 119, 122
PleI GAGTC 1 cut(s) 209
PpsI GAGTC 1 cut(s) 209
PsiI TTATAA 1 cut(s) 378
PspN4I GGNNCC 2 cut(s) 184, 258
PspPI GGNCC 3 cut(s) 61, 183, 257
SaqAI TTAA 3 cut(s) 53, 197, 296
SatI GCNGC 3 cut(s) 78, 118, 121
Sau96I GGNCC 3 cut(s) 61, 183, 257
SchI GAGTC 1 cut(s) 209
SetI ASST 4 cut(s) 70, 172, 243, 329
SfaNI GCATC 2 cut(s) 89, 385
SinI GGWCC 3 cut(s) 61, 183, 257
SmlI CTYRAG 1 cut(s) 223
SmoI CTYRAG 1 cut(s) 223
Sse9I AATT 3 cut(s) 70, 234, 307
SspMI CTAG 1 cut(s) 448
TaaI ACNGT 1 cut(s) 95
TaqI TCGA 2 cut(s) 111, 159
TasI AATT 3 cut(s) 70, 234, 307
Tru1I TTAA 3 cut(s) 53, 197, 296
Tru9I TTAA 3 cut(s) 53, 197, 296
TscAI CASTG 1 cut(s) 217
TseFI GTSAC 1 cut(s) 212
TseI GCWGC 3 cut(s) 77, 117, 120
Tsp45I GTSAC 1 cut(s) 212
TspDTI ATGAA 1 cut(s) 245
TspRI CASTG 1 cut(s) 217
VpaK11BI GGWCC 3 cut(s) 61, 183, 257
XspI CTAG 1 cut(s) 448
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.