pycom07g00630

Zinc finger A20 and AN1 domain-containing stress-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0000730
Physical Location & Seq
Reverse (-)
564688 .. 565203
516 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g00630.1

Sequence Viewer

Length: 516 bp
ATGGAGCACAACGAGACAGGATGCCAAGCTCCTCCTGAAGCTCCCAAGCTTTGTGCCAACAACTGCAGATTCTTTGGAAGTCCAGCAACCATGAATTTGTGTTCCAAGTGCCACAAGGACTTGGTGTTGAAGCAAGAACAAGCTAAAGTCGTTGCAGCATCCATTGGTAGTGTGGTGAATGGATGTCCCACTGAAAGTGGCAAGGGGCCTGTTGCTACTGCTGCTGTAGATGTACAAGCTGGTTCTGCAGATGTGATGCTTATCTCAACACAGGCTTCCTCGACGTCGTTGAACATTAAGAGTGAGGAGAAGGTGAAAGAGACTCCTACGAGGTGCGGCACTTGCAGGAAACGTGTTGGTCTAACGGGGTTCAGTTGCCGTTGTGGAGATCTCTTTTGTGCAGTTCATCGGTACTCTGATAAACACAACTGCCCCTATGATTACCGGACAGCTGCTCAGGATGCAATTGCCAAAGCCAACCCGGTTGTCAAGGCAAAAAAGCTGGATAAAATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-A20 zf-AN1

Protein Analysis

172

Amino Acids

18.19

Weight (kDa)

8.83

Isoelectric Point (pI)

34.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-A20 PF01754 17 - 39 6.9e-11 A20-like zinc finger
zf-AN1 PF01428 112 - 148 7.5e-09 AN1-like Zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 287
AciI CCGC 1 cut(s) 336
AcsI RAATTY 1 cut(s) 94
AcuI CTGAAG 1 cut(s) 57
AcyI GRCGYC 1 cut(s) 284
AfaI GTAC 2 cut(s) 234, 413
AflIII ACRYGT 1 cut(s) 352
AgsI TTSAA 2 cut(s) 130, 292
AluBI AGCT 7 cut(s) 29, 41, 49, 143, 239, 452, 502
AluI AGCT 7 cut(s) 29, 41, 49, 143, 239, 452, 502
Alw21I GWGCWC 1 cut(s) 9
Alw26I GTCTC 2 cut(s) 8, 314
AoxI GGCC 1 cut(s) 206
ApeKI GCWGC 3 cut(s) 155, 221, 452
ApoI RAATTY 1 cut(s) 94
AspS9I GGNCC 1 cut(s) 206
AsuC2I CCSGG 1 cut(s) 482
AsuHPI GGTGA 2 cut(s) 187, 325
Bbv12I GWGCWC 1 cut(s) 9
BbvI GCAGC 3 cut(s) 167, 208, 439
BceAI ACGGC 1 cut(s) 363
BcnI CCSGG 1 cut(s) 482
BcoDI GTCTC 2 cut(s) 8, 314
BfmI CTRYAG 3 cut(s) 64, 225, 246
BglII AGATCT 1 cut(s) 388
BisI GCNGC 4 cut(s) 156, 222, 337, 453
BlsI GCNGC 4 cut(s) 157, 223, 338, 454
Bme1390I CCNGG 1 cut(s) 482
BmgT120I GGNCC 1 cut(s) 206
BmiI GGNNCC 1 cut(s) 207
BmrFI CCNGG 1 cut(s) 482
BmsI GCATC 4 cut(s) 11, 167, 246, 451
Bpu10I CCTNAGC 1 cut(s) 456
BpuMI CCSGG 1 cut(s) 482
BsaBI GATNNNNATC 1 cut(s) 260
BsaHI GRCGYC 1 cut(s) 284
BsaWI WCCGGW 1 cut(s) 444
Bse8I GATNNNNATC 1 cut(s) 260
BseGI GGATG 4 cut(s) 26, 158, 188, 466
BseJI GATNNNNATC 1 cut(s) 260
BseMII CTCAG 1 cut(s) 470
BseRI GAGGAG 2 cut(s) 21, 320
BseXI GCAGC 3 cut(s) 167, 208, 439
BsgI GTGCAG 1 cut(s) 420
BshFI GGCC 1 cut(s) 208
BsiHKAI GWGCWC 1 cut(s) 9
BsiSI CCGG 2 cut(s) 445, 482
BslFI GGGAC 1 cut(s) 171
BsmAI GTCTC 2 cut(s) 8, 314
BsmFI GGGAC 1 cut(s) 171
BsnI GGCC 1 cut(s) 208
Bsp1286I GDGCHC 1 cut(s) 9
Bsp1407I TGTACA 1 cut(s) 232
Bsp143I GATC 1 cut(s) 388
BspACI CCGC 1 cut(s) 336
BspANI GGCC 1 cut(s) 208
BspCNI CTCAG 1 cut(s) 469
BspLI GGNNCC 1 cut(s) 207
BspMAI CTGCAG 2 cut(s) 68, 250
BsrGI TGTACA 1 cut(s) 232
BssMI GATC 1 cut(s) 388
BssNI GRCGYC 1 cut(s) 284
BstACI GRCGYC 1 cut(s) 284
BstAUI TGTACA 1 cut(s) 232
BstDEI CTNAG 1 cut(s) 456
BstF5I GGATG 4 cut(s) 26, 158, 188, 466
BstKTI GATC 1 cut(s) 391
BstMAI GTCTC 2 cut(s) 8, 314
BstMBI GATC 1 cut(s) 388
BstMWI GCNNNNNNNGC 4 cut(s) 221, 245, 342, 461
BstSCI CCNGG 1 cut(s) 480
BstSFI CTRYAG 3 cut(s) 64, 225, 246
BstV1I GCAGC 3 cut(s) 167, 208, 439
BstX2I RGATCY 1 cut(s) 388
BstYI RGATCY 1 cut(s) 388
BsuRI GGCC 1 cut(s) 208
BtsCI GGATG 4 cut(s) 26, 158, 188, 466
BtsIMutI CAGTG 1 cut(s) 189
Cfr13I GGNCC 1 cut(s) 206
Csp6I GTAC 2 cut(s) 233, 412
CviAII CATG 1 cut(s) 91
CviQI GTAC 2 cut(s) 233, 412
DdeI CTNAG 1 cut(s) 456
DpnI GATC 1 cut(s) 390
DpnII GATC 1 cut(s) 388
Eco57I CTGAAG 1 cut(s) 57
EcoO109I RGGNCCY 1 cut(s) 206
FaeI CATG 1 cut(s) 94
FaiI YATR 2 cut(s) 92, 438
FaqI GGGAC 1 cut(s) 171
FatI CATG 1 cut(s) 90
Fnu4HI GCNGC 4 cut(s) 156, 222, 337, 453
FokI GGATG 4 cut(s) 33, 145, 195, 473
Fsp4HI GCNGC 4 cut(s) 156, 222, 337, 453
GluI GCNGC 4 cut(s) 156, 222, 337, 453
HaeIII GGCC 1 cut(s) 208
HapII CCGG 2 cut(s) 445, 482
Hin1I GRCGYC 1 cut(s) 284
Hin1II CATG 1 cut(s) 94
HindIII AAGCTT 1 cut(s) 47
HinfI GANTC 2 cut(s) 69, 322
HpaII CCGG 2 cut(s) 445, 482
HphI GGTGA 2 cut(s) 187, 325
Hpy188I TCNGA 2 cut(s) 418, 515
Hpy188III TCNNGA 2 cut(s) 35, 458
Hpy99I CGWCG 2 cut(s) 286, 289
HpyAV CCTTC 1 cut(s) 304
HpyCH4IV ACGT 2 cut(s) 284, 352
HpyCH4V TGCA 6 cut(s) 66, 155, 248, 345, 401, 464
HpyF10VI GCNNNNNNNGC 4 cut(s) 221, 245, 342, 461
HpyF3I CTNAG 1 cut(s) 456
HpySE526I ACGT 2 cut(s) 284, 352
Hsp92I GRCGYC 1 cut(s) 284
Hsp92II CATG 1 cut(s) 94
Kzo9I GATC 1 cut(s) 388
LmnI GCTCC 3 cut(s) 4, 34, 46
Lsp1109I GCAGC 3 cut(s) 167, 208, 439
LweI GCATC 4 cut(s) 11, 167, 246, 451
MaeII ACGT 2 cut(s) 284, 352
MalI GATC 1 cut(s) 390
MboI GATC 1 cut(s) 388
MfeI CAATTG 1 cut(s) 465
MflI RGATCY 1 cut(s) 388
MhlI GDGCHC 1 cut(s) 9
MluCI AATT 2 cut(s) 94, 465
MlyI GAGTC 1 cut(s) 316
MnlI CCTC 4 cut(s) 42, 289, 298, 324
MseI TTAA 1 cut(s) 297
MspA1I CMGCKG 1 cut(s) 452
MspI CCGG 2 cut(s) 445, 482
MspR9I CCNGG 1 cut(s) 482
MunI CAATTG 1 cut(s) 465
MwoI GCNNNNNNNGC 4 cut(s) 221, 245, 342, 461
NciI CCSGG 1 cut(s) 482
NdeII GATC 1 cut(s) 388
NlaIII CATG 1 cut(s) 94
NlaIV GGNNCC 1 cut(s) 207
PfeI GAWTC 1 cut(s) 69
PkrI GCNGC 4 cut(s) 157, 223, 338, 454
PleI GAGTC 1 cut(s) 316
PpsI GAGTC 1 cut(s) 316
PspN4I GGNNCC 1 cut(s) 207
PspPI GGNCC 1 cut(s) 206
PstI CTGCAG 2 cut(s) 68, 250
PsuI RGATCY 1 cut(s) 388
PvuII CAGCTG 1 cut(s) 452
RsaI GTAC 2 cut(s) 234, 413
RsaNI GTAC 2 cut(s) 233, 412
SaqAI TTAA 1 cut(s) 297
SatI GCNGC 4 cut(s) 156, 222, 337, 453
Sau3AI GATC 1 cut(s) 388
Sau96I GGNCC 1 cut(s) 206
SchI GAGTC 1 cut(s) 316
ScrFI CCNGG 1 cut(s) 482
SduI GDGCHC 1 cut(s) 9
SfaNI GCATC 4 cut(s) 11, 167, 246, 451
SfcI CTRYAG 3 cut(s) 64, 225, 246
Sse9I AATT 2 cut(s) 94, 465
SsiI CCGC 1 cut(s) 336
StyD4I CCNGG 1 cut(s) 480
TaiI ACGT 2 cut(s) 287, 355
TaqI TCGA 1 cut(s) 281
TasI AATT 2 cut(s) 94, 465
TatI WGTACW 1 cut(s) 232
TauI GCSGC 1 cut(s) 339
TfiI GAWTC 1 cut(s) 69
Tru1I TTAA 1 cut(s) 297
Tru9I TTAA 1 cut(s) 297
TscAI CASTG 1 cut(s) 196
TseI GCWGC 3 cut(s) 155, 221, 452
TspDTI ATGAA 2 cut(s) 107, 395
TspRI CASTG 1 cut(s) 196
XapI RAATTY 1 cut(s) 94
XcmI CCANNNNNNNNNTGG 1 cut(s) 169
ZraI GACGTC 1 cut(s) 285
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.