RchiOBHm_Chr1g0314981

Zinc finger A20 and AN1 domain-containing stress-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0000730
Physical Location & Seq
Reverse (-)
2265890 .. 2266493
604 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ54553

Sequence Viewer

Length: 486 bp
ATGCTATGGGAATATTTTAAGAGGATGGAGCACAACAAGACAGGATGCCAAACACGTCCAGAAGCTCCCAAACTTTGTATCAATAACTGTGGATTCTTTGGAAGTGCAGCAACCATGAACTTGTGTTCCAAATGCCACAAGGACTTGGTATTGAAGCAAGAACAAGCCAAAGTCCCTGCAGTGTCCATTGAAAGTGCTGTGAATGCCAATCCCAATAACTGGGGAAATGAGCATGCTCTGAGGCATCCAGTACTCCGCCTCCAAACAATGAGGATATATAAGGTGAAAGCGACTCCAACCCGGTGCAACACTTGCAGGAAACGTGTTGGTATCACTGGGTTCAATTGCCGCTGTGGACATATCTTCTGTGCAGTTCATCGCTACTCTGATAAACACGACTGTCCATATGACTATCGAGCTGCCGTGCAGGATGCTATAGCAAAAGCCAACCCTGTTGTCAAGGCTGAAAAGCTGGATAAAATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-A20 zf-AN1

Protein Analysis

161

Amino Acids

18.27

Weight (kDa)

9.41

Isoelectric Point (pI)

35.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-A20 PF01754 25 - 47 1.8e-11 A20-like zinc finger
zf-AN1 PF01428 102 - 138 5.2e-09 AN1-like Zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 219
AciI CCGC 2 cut(s) 256, 349
AfaI GTAC 1 cut(s) 252
AfiI CCNNNNNNNGG 1 cut(s) 219
AflIII ACRYGT 2 cut(s) 53, 322
AgsI TTSAA 3 cut(s) 154, 191, 343
AjiI CACGTC 1 cut(s) 56
AloI GAACNNNNNNTCC 2 cut(s) 110, 142
AluBI AGCT 3 cut(s) 65, 419, 472
AluI AGCT 3 cut(s) 65, 419, 472
Alw21I GWGCWC 1 cut(s) 33
ApeKI GCWGC 2 cut(s) 107, 419
AsuC2I CCSGG 1 cut(s) 301
AsuHPI GGTGA 1 cut(s) 295
Bbv12I GWGCWC 1 cut(s) 33
BbvI GCAGC 2 cut(s) 119, 406
BccI CCATC 1 cut(s) 19
BceAI ACGGC 1 cut(s) 407
BcnI CCSGG 1 cut(s) 301
BfmI CTRYAG 2 cut(s) 177, 435
BisI GCNGC 3 cut(s) 108, 349, 420
BlsI GCNGC 3 cut(s) 109, 350, 421
BmcAI AGTACT 1 cut(s) 252
Bme1390I CCNGG 1 cut(s) 301
BmgBI CACGTC 1 cut(s) 56
BmrFI CCNGG 1 cut(s) 301
BmrI ACTGGG 2 cut(s) 229, 345
BmsI GCATC 3 cut(s) 35, 253, 421
BmuI ACTGGG 2 cut(s) 229, 345
BpuMI CCSGG 1 cut(s) 301
BsaXI ACNNNNNCTCC 2 cut(s) 243, 273
Bsc4I CCNNNNNNNGG 1 cut(s) 219
Bse1I ACTGG 3 cut(s) 224, 248, 340
BseGI GGATG 4 cut(s) 30, 50, 244, 436
BseLI CCNNNNNNNGG 1 cut(s) 219
BseMII CTCAG 1 cut(s) 230
BseNI ACTGG 3 cut(s) 224, 248, 340
BseXI GCAGC 2 cut(s) 119, 406
BsgI GTGCAG 3 cut(s) 126, 390, 446
BsiHKAI GWGCWC 1 cut(s) 33
BsiSI CCGG 1 cut(s) 301
BslFI GGGAC 1 cut(s) 158
BslI CCNNNNNNNGG 1 cut(s) 219
BsmFI GGGAC 1 cut(s) 158
BsmI GAATGC 1 cut(s) 208
Bsp1286I GDGCHC 1 cut(s) 33
BspACI CCGC 2 cut(s) 256, 349
BspCNI CTCAG 1 cut(s) 231
BspMAI CTGCAG 1 cut(s) 181
BsrI ACTGG 3 cut(s) 224, 248, 340
Bst4CI ACNGT 2 cut(s) 89, 401
BstAPI GCANNNNNTGC 1 cut(s) 312
BstC8I GCNNGC 1 cut(s) 234
BstDEI CTNAG 1 cut(s) 239
BstF5I GGATG 4 cut(s) 30, 50, 244, 436
BstMWI GCNNNNNNNGC 2 cut(s) 203, 312
BstNSI RCATGY 1 cut(s) 236
BstSCI CCNGG 1 cut(s) 299
BstSFI CTRYAG 2 cut(s) 177, 435
BstV1I GCAGC 2 cut(s) 119, 406
BtgZI GCGATG 1 cut(s) 362
BtrI CACGTC 1 cut(s) 56
BtsCI GGATG 4 cut(s) 30, 50, 244, 436
BtsI GCAGTG 1 cut(s) 186
BtsIMutI CAGTG 2 cut(s) 186, 333
Cac8I GCNNGC 1 cut(s) 234
Csp6I GTAC 1 cut(s) 251
CspCI CAANNNNNGTGG 2 cut(s) 70, 105
CviAII CATG 2 cut(s) 115, 233
CviJI RGCY 6 cut(s) 65, 167, 419, 446, 464, 472
CviKI_1 RGCY 6 cut(s) 65, 167, 419, 446, 464, 472
CviQI GTAC 1 cut(s) 251
DdeI CTNAG 1 cut(s) 239
EciI GGCGGA 1 cut(s) 245
FaeI CATG 2 cut(s) 118, 236
FaqI GGGAC 1 cut(s) 158
FatI CATG 2 cut(s) 114, 232
FauNDI CATATG 1 cut(s) 406
Fnu4HI GCNGC 3 cut(s) 108, 349, 420
FokI GGATG 4 cut(s) 37, 57, 231, 443
Fsp4HI GCNGC 3 cut(s) 108, 349, 420
GluI GCNGC 3 cut(s) 108, 349, 420
HapII CCGG 1 cut(s) 301
Hin1II CATG 2 cut(s) 118, 236
HinfI GANTC 2 cut(s) 93, 292
HpaII CCGG 1 cut(s) 301
HphI GGTGA 1 cut(s) 295
Hpy166II GTNNAC 1 cut(s) 356
Hpy188I TCNGA 2 cut(s) 240, 388
Hpy188III TCNNGA 1 cut(s) 59
Hpy8I GTNNAC 1 cut(s) 356
HpyCH4III ACNGT 2 cut(s) 89, 401
HpyCH4IV ACGT 2 cut(s) 55, 322
HpyCH4V TGCA 6 cut(s) 107, 179, 306, 315, 371, 427
HpyF10VI GCNNNNNNNGC 2 cut(s) 203, 312
HpyF3I CTNAG 1 cut(s) 239
HpySE526I ACGT 2 cut(s) 55, 322
Hsp92II CATG 2 cut(s) 118, 236
LmnI GCTCC 2 cut(s) 28, 70
Lsp1109I GCAGC 2 cut(s) 119, 406
LweI GCATC 3 cut(s) 35, 253, 421
MaeII ACGT 2 cut(s) 55, 322
MboII GAAGA 1 cut(s) 355
MfeI CAATTG 1 cut(s) 343
MhlI GDGCHC 1 cut(s) 33
MluCI AATT 1 cut(s) 343
MlyI GAGTC 1 cut(s) 286
MmeI TCCRAC 1 cut(s) 320
MnlI CCTC 4 cut(s) 15, 234, 264, 269
MseI TTAA 1 cut(s) 18
MspA1I CMGCKG 1 cut(s) 351
MspI CCGG 1 cut(s) 301
MspR9I CCNGG 1 cut(s) 301
MunI CAATTG 1 cut(s) 343
Mva1269I GAATGC 1 cut(s) 208
MwoI GCNNNNNNNGC 2 cut(s) 203, 312
NciI CCSGG 1 cut(s) 301
NdeI CATATG 1 cut(s) 406
NlaIII CATG 2 cut(s) 118, 236
NspI RCATGY 1 cut(s) 236
PaeI GCATGC 1 cut(s) 236
PctI GAATGC 1 cut(s) 208
PfeI GAWTC 1 cut(s) 93
PflMI CCANNNNNTGG 1 cut(s) 219
PkrI GCNGC 3 cut(s) 109, 350, 421
PleI GAGTC 1 cut(s) 286
PpsI GAGTC 1 cut(s) 286
PstI CTGCAG 1 cut(s) 181
RsaI GTAC 1 cut(s) 252
RsaNI GTAC 1 cut(s) 251
SaqAI TTAA 1 cut(s) 18
SatI GCNGC 3 cut(s) 108, 349, 420
ScaI AGTACT 1 cut(s) 252
SchI GAGTC 1 cut(s) 286
ScrFI CCNGG 1 cut(s) 301
SduI GDGCHC 1 cut(s) 33
SetI ASST 6 cut(s) 58, 67, 285, 325, 421, 474
SfaNI GCATC 3 cut(s) 35, 253, 421
SfcI CTRYAG 2 cut(s) 177, 435
SphI GCATGC 1 cut(s) 236
Sse9I AATT 1 cut(s) 343
SsiI CCGC 2 cut(s) 256, 349
SspI AATATT 1 cut(s) 14
StyD4I CCNGG 1 cut(s) 299
TaaI ACNGT 2 cut(s) 89, 401
TaiI ACGT 2 cut(s) 58, 325
TaqI TCGA 1 cut(s) 415
TasI AATT 1 cut(s) 343
TatI WGTACW 1 cut(s) 250
TauI GCSGC 1 cut(s) 351
TfiI GAWTC 1 cut(s) 93
Tru1I TTAA 1 cut(s) 18
Tru9I TTAA 1 cut(s) 18
TscAI CASTG 2 cut(s) 186, 340
TseI GCWGC 2 cut(s) 107, 419
TspDTI ATGAA 2 cut(s) 131, 365
TspRI CASTG 2 cut(s) 186, 340
Van91I CCANNNNNTGG 1 cut(s) 219
XceI RCATGY 1 cut(s) 236
ZrmI AGTACT 1 cut(s) 252
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.