RLG00000003146

Zinc finger A20 and AN1 domain-containing stress-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0000730
Physical Location & Seq
Forward (+)
44867344 .. 44867904
561 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000003146

Sequence Viewer

Length: 561 bp
ATGAACCAACATGAGGAAACTGGATGTCAAGCTCCCCCTCAAGGTGCTAAATTATGCATAAAAAACTGTGGTTTCTTTGGAGGTGCGGCTACTATGAACATGTGTTCCAAGTGCCACAAAGACTCGATGAAACAGGAGCAGACTAAGCTTGCTGCATCATCCATTGGAAATACTGTCGATGGAAAATCAAAGGAATCTATTGTTTCTGCTAATGTGGATGTTCAAGCTAATTCAGTGGGCCCAAAAACTATTTTGATGAACTCCATCTTTTCTTTTAGGTCGCAGCAGTATGATGTCTCTGATCCTCAACCATCCTCCTTGACTTTCGGGACATGCCAGAATAACGAGGAAGCGAAGCAGCCGGAGGGTCCGAAACGGTGCACTAGCTGCAACAAGCGGGTTGGTTTGACAGGGTTCAACTGTCGTTGTGGTCACCTTTTTTGTGCAGTACATCGTTATTCGGAGGAACATGATTGCTCTTATGATCATCGTACTGCTGGTCGTGAGGCCATTTCTAAAGCCAATCCTATTGTCAAAGCTGAGAAGCTTGATAAAATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-A20 zf-AN1

Protein Analysis

187

Amino Acids

20.26

Weight (kDa)

8.43

Isoelectric Point (pI)

40.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-A20 PF01754 18 - 40 2.3e-09 A20-like zinc finger
zf-AN1 PF01428 127 - 163 2.2e-08 AN1-like Zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 86, 397
AclWI GGATC 1 cut(s) 296
AfaI GTAC 2 cut(s) 450, 493
AfiI CCNNNNNNNGG 2 cut(s) 13, 41
AflIII ACRYGT 1 cut(s) 99
AgsI TTSAA 2 cut(s) 224, 418
AloI GAACNNNNNNTCC 2 cut(s) 89, 121
AluBI AGCT 6 cut(s) 32, 148, 227, 387, 539, 547
AluI AGCT 6 cut(s) 32, 148, 227, 387, 539, 547
Alw21I GWGCWC 1 cut(s) 383
Alw26I GTCTC 1 cut(s) 301
Alw44I GTGCAC 1 cut(s) 379
AlwI GGATC 1 cut(s) 296
AoxI GGCC 2 cut(s) 238, 507
ApaI GGGCCC 1 cut(s) 242
ApaLI GTGCAC 1 cut(s) 379
ApeKI GCWGC 4 cut(s) 152, 283, 358, 387
AspS9I GGNCC 3 cut(s) 238, 239, 368
AsuHPI GGTGA 1 cut(s) 425
AvaII GGWCC 1 cut(s) 368
BaeGI GKGCMC 2 cut(s) 242, 383
BanII GRGCYC 1 cut(s) 242
Bbv12I GWGCWC 1 cut(s) 383
BbvI GCAGC 4 cut(s) 139, 295, 370, 374
BccI CCATC 3 cut(s) 173, 272, 319
BclI TGATCA 1 cut(s) 484
BcoDI GTCTC 1 cut(s) 301
BfaI CTAG 1 cut(s) 384
BisI GCNGC 5 cut(s) 87, 153, 284, 359, 388
BlsI GCNGC 5 cut(s) 88, 154, 285, 360, 389
Bme18I GGWCC 1 cut(s) 368
BmgT120I GGNCC 3 cut(s) 238, 239, 368
BmiI GGNNCC 2 cut(s) 240, 369
BmsI GCATC 1 cut(s) 164
BpuEI CTTGAG 1 cut(s) 24
Bsc4I CCNNNNNNNGG 2 cut(s) 13, 41
Bse1I ACTGG 1 cut(s) 25
BseGI GGATG 4 cut(s) 29, 158, 223, 311
BseLI CCNNNNNNNGG 2 cut(s) 13, 41
BseMII CTCAG 1 cut(s) 531
BseNI ACTGG 1 cut(s) 25
BseSI GKGCMC 2 cut(s) 242, 383
BseXI GCAGC 4 cut(s) 139, 295, 370, 374
BsgI GTGCAG 1 cut(s) 465
BshFI GGCC 2 cut(s) 240, 509
BsiHKAI GWGCWC 1 cut(s) 383
BsiSI CCGG 1 cut(s) 362
BslFI GGGAC 1 cut(s) 343
BslI CCNNNNNNNGG 2 cut(s) 13, 41
BsmAI GTCTC 1 cut(s) 301
BsmFI GGGAC 1 cut(s) 343
BsnI GGCC 2 cut(s) 240, 509
Bsp120I GGGCCC 1 cut(s) 238
Bsp1286I GDGCHC 2 cut(s) 242, 383
Bsp143I GATC 2 cut(s) 301, 484
BspACI CCGC 2 cut(s) 86, 397
BspANI GGCC 2 cut(s) 240, 509
BspCNI CTCAG 1 cut(s) 532
BspLI GGNNCC 2 cut(s) 240, 369
BspPI GGATC 1 cut(s) 296
BsrI ACTGG 1 cut(s) 25
BssMI GATC 2 cut(s) 301, 484
Bst4CI ACNGT 4 cut(s) 68, 175, 378, 422
BstAPI GCANNNNNTGC 1 cut(s) 387
BstC8I GCNNGC 1 cut(s) 150
BstDEI CTNAG 2 cut(s) 144, 540
BstEII GGTNACC 1 cut(s) 431
BstF5I GGATG 4 cut(s) 29, 158, 223, 311
BstKTI GATC 2 cut(s) 304, 487
BstMAI GTCTC 1 cut(s) 301
BstMBI GATC 2 cut(s) 301, 484
BstMWI GCNNNNNNNGC 2 cut(s) 145, 387
BstNSI RCATGY 2 cut(s) 103, 336
BstPI GGTNACC 1 cut(s) 431
BstSLI GKGCMC 2 cut(s) 242, 383
BstV1I GCAGC 4 cut(s) 139, 295, 370, 374
BsuRI GGCC 2 cut(s) 240, 509
BtsCI GGATG 4 cut(s) 29, 158, 223, 311
BtsIMutI CAGTG 1 cut(s) 240
Cac8I GCNNGC 1 cut(s) 150
Cfr13I GGNCC 3 cut(s) 238, 239, 368
Csp6I GTAC 2 cut(s) 449, 492
CviAII CATG 4 cut(s) 11, 100, 333, 470
CviQI GTAC 2 cut(s) 449, 492
DdeI CTNAG 2 cut(s) 144, 540
DpnI GATC 2 cut(s) 303, 486
DpnII GATC 2 cut(s) 301, 484
Eco24I GRGCYC 1 cut(s) 242
Eco47I GGWCC 1 cut(s) 368
Eco91I GGTNACC 1 cut(s) 431
EcoO65I GGTNACC 1 cut(s) 431
EcoT22I ATGCAT 1 cut(s) 59
EcoT38I GRGCYC 1 cut(s) 242
FaeI CATG 4 cut(s) 14, 103, 336, 473
FaiI YATR 9 cut(s) 12, 55, 59, 95, 101, 291, 334, 471, 483
FaqI GGGAC 1 cut(s) 343
FatI CATG 4 cut(s) 10, 99, 332, 469
FauI CCCGC 1 cut(s) 390
FbaI TGATCA 1 cut(s) 484
Fnu4HI GCNGC 5 cut(s) 87, 153, 284, 359, 388
FokI GGATG 4 cut(s) 36, 145, 230, 298
FriOI GRGCYC 1 cut(s) 242
Fsp4HI GCNGC 5 cut(s) 87, 153, 284, 359, 388
FspBI CTAG 1 cut(s) 384
GluI GCNGC 5 cut(s) 87, 153, 284, 359, 388
HaeIII GGCC 2 cut(s) 240, 509
HapII CCGG 1 cut(s) 362
Hin1II CATG 4 cut(s) 14, 103, 336, 473
HindIII AAGCTT 2 cut(s) 146, 545
HinfI GANTC 2 cut(s) 122, 194
HpaII CCGG 1 cut(s) 362
HphI GGTGA 1 cut(s) 425
Hpy166II GTNNAC 1 cut(s) 381
Hpy188I TCNGA 3 cut(s) 301, 372, 463
Hpy188III TCNNGA 2 cut(s) 328, 503
Hpy8I GTNNAC 1 cut(s) 381
HpyCH4III ACNGT 4 cut(s) 68, 175, 378, 422
HpyCH4V TGCA 5 cut(s) 57, 155, 381, 390, 446
HpyF10VI GCNNNNNNNGC 2 cut(s) 145, 387
HpyF3I CTNAG 2 cut(s) 144, 540
Hsp92II CATG 4 cut(s) 14, 103, 336, 473
Ksp22I TGATCA 1 cut(s) 484
Kzo9I GATC 2 cut(s) 301, 484
LmnI GCTCC 2 cut(s) 37, 136
LpnPI CCDG 6 cut(s) 6, 119, 350, 375, 396, 483
Lsp1109I GCAGC 4 cut(s) 139, 295, 370, 374
LweI GCATC 1 cut(s) 164
MaeI CTAG 1 cut(s) 384
MaeIII GTNAC 1 cut(s) 431
MalI GATC 2 cut(s) 303, 486
MboI GATC 2 cut(s) 301, 484
MhlI GDGCHC 2 cut(s) 242, 383
MluCI AATT 2 cut(s) 50, 229
MlyI GAGTC 1 cut(s) 116
MnlI CCTC 9 cut(s) 7, 48, 74, 315, 325, 340, 358, 457, 499
Mph1103I ATGCAT 1 cut(s) 59
MspI CCGG 1 cut(s) 362
MwoI GCNNNNNNNGC 2 cut(s) 145, 387
NdeII GATC 2 cut(s) 301, 484
NlaIII CATG 4 cut(s) 14, 103, 336, 473
NlaIV GGNNCC 2 cut(s) 240, 369
NmuCI GTSAC 1 cut(s) 431
NsiI ATGCAT 1 cut(s) 59
NspI RCATGY 2 cut(s) 103, 336
PciI ACATGT 1 cut(s) 99
PfeI GAWTC 1 cut(s) 194
PkrI GCNGC 5 cut(s) 88, 154, 285, 360, 389
PleI GAGTC 1 cut(s) 116
PpsI GAGTC 1 cut(s) 116
PscI ACATGT 1 cut(s) 99
PspEI GGTNACC 1 cut(s) 431
PspN4I GGNNCC 2 cut(s) 240, 369
PspOMI GGGCCC 1 cut(s) 238
PspPI GGNCC 3 cut(s) 238, 239, 368
RsaI GTAC 2 cut(s) 450, 493
RsaNI GTAC 2 cut(s) 449, 492
SatI GCNGC 5 cut(s) 87, 153, 284, 359, 388
Sau3AI GATC 2 cut(s) 301, 484
Sau96I GGNCC 3 cut(s) 238, 239, 368
SchI GAGTC 1 cut(s) 116
SduI GDGCHC 2 cut(s) 242, 383
SfaNI GCATC 1 cut(s) 164
SinI GGWCC 1 cut(s) 368
SmlI CTYRAG 1 cut(s) 39
SmoI CTYRAG 1 cut(s) 39
Sse9I AATT 2 cut(s) 50, 229
SsiI CCGC 2 cut(s) 86, 397
SspMI CTAG 1 cut(s) 384
TaaI ACNGT 4 cut(s) 68, 175, 378, 422
TaqI TCGA 2 cut(s) 125, 177
TasI AATT 2 cut(s) 50, 229
TatI WGTACW 1 cut(s) 448
TauI GCSGC 1 cut(s) 89
TfiI GAWTC 1 cut(s) 194
TscAI CASTG 1 cut(s) 240
TseFI GTSAC 1 cut(s) 431
TseI GCWGC 4 cut(s) 152, 283, 358, 387
Tsp45I GTSAC 1 cut(s) 431
TspDTI ATGAA 4 cut(s) 17, 110, 143, 272
TspRI CASTG 1 cut(s) 240
VneI GTGCAC 1 cut(s) 379
VpaK11BI GGWCC 1 cut(s) 368
XceI RCATGY 2 cut(s) 103, 336
XspI CTAG 1 cut(s) 384
Zsp2I ATGCAT 1 cut(s) 59
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.