RLG00000023442

Zinc finger A20 and AN1 domain-containing stress-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0000730
Physical Location & Seq
Forward (+)
23821443 .. 23825362
3920 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000023442

Sequence Viewer

Length: 921 bp
ATGCCCAAACTCGGGGAGCCCAGCGCAAGGAGCCCAACAATAGTCAGCCTAGCAGGAGGACAAGGCAGCCCAATCAGCTTGGATGCCATCGCCGATCTCCCTAGCTCATCTCAGAGTTCCAGCCACCAACGCCAGCACCGCCGCTGGTGCCTCTACCAAGTGCAGCCCATGCCCAGCCATGGAACTCTGCAAGCACTGACGACGAAGGCTCTGCAGAAGCCGTCACCACCGCTCCTCGCTCCGGATCTGCCTCCGACACCCGAAATCAGGTCTTACCTCCCTTCCAGCCTCGACAACTCCCGTCGATTCCTCAATCAAAACCATCCGCCACCAGATTGTCTCCCCCACGAGACGACAACGGAAACAGTCAATGACCAATCGGCCAAGGCAACCAGATTAACAAAGATGGACTCTCACGATGAAACTGGATGCCAAGCACCAGACCGCCCCATCCTTTGCATTAATAACTGTGGCTTCTTCGGAAGGGCAGCTACAATGAATATGTGTTCCAAATGTTACAAGGACATGCTTCTAAAGCAGGAGCAGGCCAATCTGGCAGCAACATCCATTGGTAGCCTTGTGAATGGCAACAATAGTGGCATTGGCCCTGTTGTTGCTAATGCTGTCGATGTGCAAGCTGGACAAGTTGAGGCAGTGGTTATTTCAACAGAGCCATCTTGTGGCTCATCCTCAAGCAAAGTTACTGATGAGGTGAAAGAAATAGCGGGACCAAAGAGATGCACTACTTGCCGAAAGCGTGTTGGTCTAACTGGGTTCAATTGCAAATGTGGAAACACCTTCTGTTCAACTCATCGCTATTCTGACAAACATGACTGCCCTTTTGATTATAGGACTGCTGGTCGGGATGCTATTGCTAAAGCCAATCCTGTTGTCAAGGCAGAGAAACTTGATAAGATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-A20 zf-AN1

Protein Analysis

307

Amino Acids

32.9

Weight (kDa)

8.62

Isoelectric Point (pI)

56.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-A20 PF01754 151 - 174 1.5e-11 A20-like zinc finger
zf-AN1 PF01428 247 - 283 8.1e-10 AN1-like Zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 147
AccB7I CCANNNNNTGG 1 cut(s) 680
AccBSI CCGCTC 1 cut(s) 232
AccIII TCCGGA 1 cut(s) 241
AciI CCGC 6 cut(s) 139, 142, 230, 326, 445, 725
AclWI GGATC 1 cut(s) 252
AcoI YGGCCR 1 cut(s) 381
AfiI CCNNNNNNNGG 7 cut(s) 11, 12, 27, 179, 241, 267, 680
AgsI TTSAA 3 cut(s) 666, 778, 807
AhdI GACNNNNNGTC 1 cut(s) 858
AluBI AGCT 4 cut(s) 78, 105, 491, 638
AluI AGCT 4 cut(s) 78, 105, 491, 638
Alw26I GTCTC 2 cut(s) 344, 344
AlwI GGATC 1 cut(s) 252
Ama87I CYCGRG 1 cut(s) 11
Aor13HI TCCGGA 1 cut(s) 241
AoxI GGCC 3 cut(s) 381, 546, 604
ApeKI GCWGC 4 cut(s) 66, 163, 488, 557
ArsI GACNNNNNNTTYG 2 cut(s) 824, 856
AseI ATTAAT 1 cut(s) 462
AspLEI GCGC 1 cut(s) 26
AspS9I GGNCC 2 cut(s) 605, 728
AsuHPI GGTGA 2 cut(s) 216, 724
AvaI CYCGRG 1 cut(s) 11
AvaII GGWCC 1 cut(s) 728
BanI GGYRCC 1 cut(s) 147
BanII GRGCYC 2 cut(s) 21, 35
BarI GAAGNNNNNNTAC 2 cut(s) 475, 507
BauI CACGAG 1 cut(s) 347
BbvI GCAGC 4 cut(s) 78, 175, 500, 569
BccI CCATC 5 cut(s) 95, 330, 400, 458, 682
BceAI ACGGC 1 cut(s) 205
BcgI CGANNNNNNTGC 2 cut(s) 193, 227
BcoDI GTCTC 2 cut(s) 344, 344
BfaI CTAG 3 cut(s) 50, 102, 919
BfmI CTRYAG 1 cut(s) 212
BglI GCCNNNNNGGC 1 cut(s) 554
BglII AGATCT 1 cut(s) 915
BisI GCNGC 5 cut(s) 67, 142, 164, 489, 558
BlsI GCNGC 5 cut(s) 68, 143, 165, 490, 559
Bme18I GGWCC 1 cut(s) 728
BmeRI GACNNNNNGTC 1 cut(s) 858
BmeT110I CYCGRG 1 cut(s) 11
BmgT120I GGNCC 2 cut(s) 605, 728
BmiI GGNNCC 4 cut(s) 18, 32, 149, 729
BmrI ACTGGG 1 cut(s) 780
BmsI GCATC 4 cut(s) 73, 419, 728, 856
BmuI ACTGGG 1 cut(s) 780
BpuEI CTTGAG 1 cut(s) 676
BsaJI CCNNGG 2 cut(s) 178, 384
BsaWI WCCGGW 1 cut(s) 241
BsaXI ACNNNNNCTCC 2 cut(s) 216, 246
Bsc4I CCNNNNNNNGG 7 cut(s) 11, 12, 27, 179, 241, 267, 680
Bse1I ACTGG 2 cut(s) 430, 775
BseAI TCCGGA 1 cut(s) 241
BseDI CCNNGG 2 cut(s) 178, 384
BseGI GGATG 7 cut(s) 88, 322, 434, 450, 563, 686, 871
BseLI CCNNNNNNNGG 7 cut(s) 11, 12, 27, 179, 241, 267, 680
BseMII CTCAG 1 cut(s) 125
BseNI ACTGG 2 cut(s) 430, 775
BseRI GAGGAG 1 cut(s) 224
BseXI GCAGC 4 cut(s) 78, 175, 500, 569
BseYI CCCAGC 2 cut(s) 20, 173
BsgI GTGCAG 1 cut(s) 182
BshFI GGCC 3 cut(s) 383, 548, 606
BshNI GGYRCC 1 cut(s) 147
BsiHKCI CYCGRG 1 cut(s) 11
BsiSI CCGG 1 cut(s) 242
BslFI GGGAC 1 cut(s) 741
BslI CCNNNNNNNGG 7 cut(s) 11, 12, 27, 179, 241, 267, 680
BsmAI GTCTC 2 cut(s) 344, 344
BsmBI CGTCTC 1 cut(s) 344
BsmFI GGGAC 1 cut(s) 741
BsnI GGCC 3 cut(s) 383, 548, 606
BsoBI CYCGRG 1 cut(s) 11
Bsp1286I GDGCHC 2 cut(s) 21, 35
Bsp13I TCCGGA 1 cut(s) 241
Bsp143I GATC 3 cut(s) 94, 244, 915
Bsp19I CCATGG 1 cut(s) 178
BspACI CCGC 6 cut(s) 139, 142, 230, 326, 445, 725
BspANI GGCC 3 cut(s) 383, 548, 606
BspCNI CTCAG 1 cut(s) 124
BspEI TCCGGA 1 cut(s) 241
BspLI GGNNCC 4 cut(s) 18, 32, 149, 729
BspMAI CTGCAG 1 cut(s) 216
BspPI GGATC 1 cut(s) 252
BspT107I GGYRCC 1 cut(s) 147
BsrBI CCGCTC 1 cut(s) 232
BsrI ACTGG 2 cut(s) 430, 775
BssECI CCNNGG 2 cut(s) 178, 384
BssMI GATC 3 cut(s) 94, 244, 915
BssSI CACGAG 1 cut(s) 347
BssT1I CCWWGG 2 cut(s) 178, 384
Bst2BI CACGAG 1 cut(s) 347
Bst4CI ACNGT 2 cut(s) 367, 470
BstAPI GCANNNNNTGC 2 cut(s) 169, 747
BstC8I GCNNGC 4 cut(s) 134, 192, 546, 636
BstDEI CTNAG 1 cut(s) 111
BstDSI CCRYGG 1 cut(s) 178
BstF5I GGATG 7 cut(s) 88, 322, 434, 450, 563, 686, 871
BstHHI GCGC 1 cut(s) 26
BstKTI GATC 3 cut(s) 97, 247, 918
BstMAI GTCTC 2 cut(s) 344, 344
BstMBI GATC 3 cut(s) 94, 244, 915
BstMWI GCNNNNNNNGC 9 cut(s) 30, 75, 129, 138, 147, 169, 535, 554, 747
BstNSI RCATGY 1 cut(s) 529
BstSFI CTRYAG 1 cut(s) 212
BstV1I GCAGC 4 cut(s) 78, 175, 500, 569
BstX2I RGATCY 2 cut(s) 244, 915
BstYI RGATCY 2 cut(s) 244, 915
BsuRI GGCC 3 cut(s) 383, 548, 606
BtgI CCRYGG 1 cut(s) 178
BtgZI GCGATG 2 cut(s) 73, 797
BtsCI GGATG 7 cut(s) 88, 322, 434, 450, 563, 686, 871
BtsI GCAGTG 1 cut(s) 660
BtsIMutI CAGTG 2 cut(s) 194, 660
Cac8I GCNNGC 4 cut(s) 134, 192, 546, 636
CfoI GCGC 1 cut(s) 26
Cfr13I GGNCC 2 cut(s) 605, 728
CspCI CAANNNNNGTGG 2 cut(s) 577, 612
CviAII CATG 4 cut(s) 169, 179, 526, 830
DdeI CTNAG 1 cut(s) 111
DpnI GATC 3 cut(s) 96, 246, 917
DpnII GATC 3 cut(s) 94, 244, 915
DriI GACNNNNNGTC 1 cut(s) 858
EaeI YGGCCR 1 cut(s) 381
Eam1105I GACNNNNNGTC 1 cut(s) 858
EciI GGCGGA 1 cut(s) 315
Eco130I CCWWGG 2 cut(s) 178, 384
Eco24I GRGCYC 2 cut(s) 21, 35
Eco47I GGWCC 1 cut(s) 728
Eco88I CYCGRG 1 cut(s) 11
EcoT14I CCWWGG 2 cut(s) 178, 384
EcoT38I GRGCYC 2 cut(s) 21, 35
ErhI CCWWGG 2 cut(s) 178, 384
Esp3I CGTCTC 1 cut(s) 344
FaeI CATG 4 cut(s) 172, 182, 529, 833
FaiI YATR 6 cut(s) 170, 180, 503, 527, 831, 849
FaqI GGGAC 1 cut(s) 741
FatI CATG 4 cut(s) 168, 178, 525, 829
FauI CCCGC 1 cut(s) 718
Fnu4HI GCNGC 5 cut(s) 67, 142, 164, 489, 558
FokI GGATG 7 cut(s) 95, 309, 437, 441, 550, 673, 878
FriOI GRGCYC 2 cut(s) 21, 35
Fsp4HI GCNGC 5 cut(s) 67, 142, 164, 489, 558
FspBI CTAG 3 cut(s) 50, 102, 919
GlaI GCGC 1 cut(s) 25
GluI GCNGC 5 cut(s) 67, 142, 164, 489, 558
GsaI CCCAGC 2 cut(s) 24, 177
HaeIII GGCC 3 cut(s) 383, 548, 606
HapII CCGG 1 cut(s) 242
HhaI GCGC 1 cut(s) 26
Hin1II CATG 4 cut(s) 172, 182, 529, 833
Hin6I GCGC 1 cut(s) 24
HinP1I GCGC 1 cut(s) 24
HinfI GANTC 2 cut(s) 306, 410
HpaII CCGG 1 cut(s) 242
HphI GGTGA 2 cut(s) 216, 724
Hpy188I TCNGA 4 cut(s) 114, 255, 482, 823
Hpy188III TCNNGA 3 cut(s) 242, 416, 863
Hpy99I CGWCG 2 cut(s) 205, 306
HpyAV CCTTC 4 cut(s) 199, 291, 477, 808
HpyCH4III ACNGT 2 cut(s) 367, 470
HpyCH4V TGCA 7 cut(s) 163, 190, 214, 459, 634, 741, 783
HpyF10VI GCNNNNNNNGC 9 cut(s) 30, 75, 129, 138, 147, 169, 535, 554, 747
HpyF3I CTNAG 1 cut(s) 111
Hsp92II CATG 4 cut(s) 172, 182, 529, 833
HspAI GCGC 1 cut(s) 24
Kpn2I TCCGGA 1 cut(s) 241
Kzo9I GATC 3 cut(s) 94, 244, 915
LmnI GCTCC 5 cut(s) 16, 30, 237, 244, 541
Lsp1109I GCAGC 4 cut(s) 78, 175, 500, 569
LweI GCATC 4 cut(s) 73, 419, 728, 856
MaeI CTAG 3 cut(s) 50, 102, 919
MaeIII GTNAC 3 cut(s) 222, 515, 700
MalI GATC 3 cut(s) 96, 246, 917
MbiI CCGCTC 1 cut(s) 232
MboI GATC 3 cut(s) 94, 244, 915
MboII GAAGA 1 cut(s) 469
MfeI CAATTG 1 cut(s) 778
MflI RGATCY 2 cut(s) 244, 915
MhlI GDGCHC 2 cut(s) 21, 35
MluCI AATT 1 cut(s) 778
MlyI GAGTC 1 cut(s) 404
MmeI TCCRAC 1 cut(s) 278
MroI TCCGGA 1 cut(s) 241
MseI TTAA 2 cut(s) 398, 462
MspA1I CMGCKG 1 cut(s) 144
MspI CCGG 1 cut(s) 242
MunI CAATTG 1 cut(s) 778
MwoI GCNNNNNNNGC 9 cut(s) 30, 75, 129, 138, 147, 169, 535, 554, 747
NcoI CCATGG 1 cut(s) 178
NdeII GATC 3 cut(s) 94, 244, 915
NlaIII CATG 4 cut(s) 172, 182, 529, 833
NlaIV GGNNCC 4 cut(s) 18, 32, 149, 729
NmuCI GTSAC 1 cut(s) 222
NspI RCATGY 1 cut(s) 529
PfeI GAWTC 1 cut(s) 306
PflMI CCANNNNNTGG 1 cut(s) 680
PkrI GCNGC 5 cut(s) 68, 143, 165, 490, 559
PleI GAGTC 1 cut(s) 404
PpsI GAGTC 1 cut(s) 404
PshBI ATTAAT 1 cut(s) 462
PspFI CCCAGC 2 cut(s) 20, 173
PspN4I GGNNCC 4 cut(s) 18, 32, 149, 729
PspPI GGNCC 2 cut(s) 605, 728
PstI CTGCAG 1 cut(s) 216
PsuI RGATCY 2 cut(s) 244, 915
SaqAI TTAA 2 cut(s) 398, 462
SatI GCNGC 5 cut(s) 67, 142, 164, 489, 558
Sau3AI GATC 3 cut(s) 94, 244, 915
Sau96I GGNCC 2 cut(s) 605, 728
SchI GAGTC 1 cut(s) 404
SduI GDGCHC 2 cut(s) 21, 35
SetI ASST 8 cut(s) 80, 107, 272, 279, 493, 640, 714, 800
SfaNI GCATC 4 cut(s) 73, 419, 728, 856
SfcI CTRYAG 1 cut(s) 212
SinI GGWCC 1 cut(s) 728
SmlI CTYRAG 1 cut(s) 691
SmoI CTYRAG 1 cut(s) 691
Sse9I AATT 1 cut(s) 778
SsiI CCGC 6 cut(s) 139, 142, 230, 326, 445, 725
SspMI CTAG 3 cut(s) 50, 102, 919
StyI CCWWGG 2 cut(s) 178, 384
TaaI ACNGT 2 cut(s) 367, 470
TaqI TCGA 3 cut(s) 291, 304, 627
TasI AATT 1 cut(s) 778
TauI GCSGC 1 cut(s) 144
TfiI GAWTC 1 cut(s) 306
Tru1I TTAA 2 cut(s) 398, 462
Tru9I TTAA 2 cut(s) 398, 462
TscAI CASTG 2 cut(s) 201, 660
TseFI GTSAC 1 cut(s) 222
TseI GCWGC 4 cut(s) 66, 163, 488, 557
Tsp45I GTSAC 1 cut(s) 222
TspDTI ATGAA 2 cut(s) 435, 512
TspGWI ACGGA 1 cut(s) 374
TspRI CASTG 2 cut(s) 201, 660
Van91I CCANNNNNTGG 1 cut(s) 680
VpaK11BI GGWCC 1 cut(s) 728
VspI ATTAAT 1 cut(s) 462
XceI RCATGY 1 cut(s) 529
XspI CTAG 3 cut(s) 50, 102, 919
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.