Rorug05G0452500

Zinc finger A20 and AN1 domain-containing stress-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0000730
Physical Location & Seq
Forward (+)
62557127 .. 62557750
624 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0452500.1

Sequence Viewer

Length: 624 bp
ATGGAACCACAAACCCAGCAACCAAGGAGACCACAAACCCAGCAACCAACGAGACCACAAACCCAGCAACTAAGCAAGGATGGAGGAAGCAACAAAGCAGCCGGGAGTAGTGCTAACATGCTTTGCAGTCGGAGCAGTACCAGGTGGACTCCCACTCCAGATCAGATAAGAATCCTCAATGAGCTTTTCTATAGCAAGGGAGTTAGGTCCCCAACTGTAGAGCAAGTTCAGCGGATCTGTTTCCAGCTGAGACGGTACGGACAGATCGAGGCCAAGAATGTCTTTTATTGGTTCCAGCACCACAAGGCTCGGGATAAACGGAAGAAGATCACTTCGGATGTTCTTGTGCCCATGCAAAGATCAGGGCTTGTTGGTAATGGTACCAATTGGAAACCTGAGGATAAGTATCTTAACTTCGGATCTTCTGCATCTGCTTGTGCTTCTTCAGCTGGTGTTGGTGGATCCATAAAGGATATTGAAACCCTTCCACTATTCCCCATGCACAGTGAAGACATCTTGGGCAACATGAAGACTACTTCCGAGGGAGGTGGCGGCTATGGCGGTGGCTCTCGCATTTCCCTTGAGCTCAGCCTCAACTCCTCCGGATCGGTTAACTTGACTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-A20 zf-AN1

Protein Analysis

207

Amino Acids

22.74

Weight (kDa)

9.78

Isoelectric Point (pI)

61.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Homeodomain PF00046 47 - 107 7e-11 Homeodomain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 380
AccB1I GGYRCC 1 cut(s) 380
AccIII TCCGGA 1 cut(s) 602
AciI CCGC 3 cut(s) 232, 552, 561
AclWI GGATC 5 cut(s) 242, 427, 456, 469, 613
AcuI CTGAAG 1 cut(s) 429
AfaI GTAC 3 cut(s) 139, 257, 382
AgsI TTSAA 1 cut(s) 479
AjnI CCWGG 1 cut(s) 140
AluBI AGCT 4 cut(s) 184, 247, 449, 586
AluI AGCT 4 cut(s) 184, 247, 449, 586
Alw21I GWGCWC 1 cut(s) 588
Alw26I GTCTC 3 cut(s) 22, 46, 244
AlwI GGATC 5 cut(s) 242, 427, 456, 469, 613
Ama87I CYCGRG 1 cut(s) 309
Aor13HI TCCGGA 1 cut(s) 602
AoxI GGCC 1 cut(s) 270
ApeKI GCWGC 1 cut(s) 98
Asp700I GAANNNNTTC 1 cut(s) 483
Asp718I GGTACC 1 cut(s) 380
AspS9I GGNCC 1 cut(s) 207
AsuC2I CCSGG 1 cut(s) 103
AvaI CYCGRG 1 cut(s) 309
AvaII GGWCC 1 cut(s) 207
AxyI CCTNAGG 1 cut(s) 396
BaeGI GKGCMC 1 cut(s) 351
BamHI GGATCC 1 cut(s) 461
BanI GGYRCC 1 cut(s) 380
BanII GRGCYC 1 cut(s) 588
BarI GAAGNNNNNNTAC 2 cut(s) 398, 430
BbsI GAAGAC 2 cut(s) 516, 536
Bbv12I GWGCWC 1 cut(s) 588
BbvI GCAGC 1 cut(s) 110
BccI CCATC 1 cut(s) 74
BciT130I CCWGG 1 cut(s) 142
BcnI CCSGG 1 cut(s) 103
BcoDI GTCTC 3 cut(s) 22, 46, 244
BfmI CTRYAG 2 cut(s) 190, 216
BisI GCNGC 2 cut(s) 99, 553
BlpI GCTNAGC 1 cut(s) 587
BlsI GCNGC 2 cut(s) 100, 554
Bme1390I CCNGG 2 cut(s) 103, 142
Bme18I GGWCC 1 cut(s) 207
BmeT110I CYCGRG 1 cut(s) 309
BmgT120I GGNCC 1 cut(s) 207
BmiI GGNNCC 5 cut(s) 6, 209, 293, 382, 463
BmrFI CCNGG 2 cut(s) 103, 142
BmsI GCATC 1 cut(s) 437
BpiI GAAGAC 2 cut(s) 516, 536
BpmI CTGGAG 1 cut(s) 141
Bpu1102I GCTNAGC 1 cut(s) 587
BpuEI CTTGAG 1 cut(s) 602
BpuMI CCSGG 1 cut(s) 103
BsaBI GATNNNNATC 2 cut(s) 170, 405
BsaI GGTCTC 2 cut(s) 22, 46
BsaJI CCNNGG 2 cut(s) 23, 540
BsaWI WCCGGW 1 cut(s) 602
BsaXI ACNNNNNCTCC 2 cut(s) 139, 169
Bse21I CCTNAGG 1 cut(s) 396
Bse8I GATNNNNATC 2 cut(s) 170, 405
BseAI TCCGGA 1 cut(s) 602
BseBI CCWGG 1 cut(s) 142
BseDI CCNNGG 2 cut(s) 23, 540
BseGI GGATG 2 cut(s) 85, 343
BseJI GATNNNNATC 2 cut(s) 170, 405
BseMII CTCAG 3 cut(s) 239, 387, 601
BseRI GAGGAG 1 cut(s) 589
BseSI GKGCMC 1 cut(s) 351
BseXI GCAGC 1 cut(s) 110
BseYI CCCAGC 3 cut(s) 15, 39, 63
BshFI GGCC 1 cut(s) 272
BshNI GGYRCC 1 cut(s) 380
BsiHKAI GWGCWC 1 cut(s) 588
BsiHKCI CYCGRG 1 cut(s) 309
BsiSI CCGG 2 cut(s) 102, 603
BslFI GGGAC 1 cut(s) 193
BsmAI GTCTC 3 cut(s) 22, 46, 244
BsmBI CGTCTC 1 cut(s) 244
BsmFI GGGAC 1 cut(s) 193
BsnI GGCC 1 cut(s) 272
Bso31I GGTCTC 2 cut(s) 22, 46
BsoBI CYCGRG 1 cut(s) 309
Bsp1286I GDGCHC 2 cut(s) 351, 588
Bsp13I TCCGGA 1 cut(s) 602
Bsp143I GATC 8 cut(s) 160, 234, 264, 327, 359, 419, 461, 605
Bsp1720I GCTNAGC 1 cut(s) 587
BspACI CCGC 3 cut(s) 232, 552, 561
BspANI GGCC 1 cut(s) 272
BspCNI CTCAG 3 cut(s) 240, 388, 600
BspEI TCCGGA 1 cut(s) 602
BspLI GGNNCC 5 cut(s) 6, 209, 293, 382, 463
BspPI GGATC 5 cut(s) 242, 427, 456, 469, 613
BspT107I GGYRCC 1 cut(s) 380
BspTNI GGTCTC 2 cut(s) 22, 46
BssECI CCNNGG 2 cut(s) 23, 540
BssMI GATC 8 cut(s) 160, 234, 264, 327, 359, 419, 461, 605
BssT1I CCWWGG 1 cut(s) 23
Bst2UI CCWGG 1 cut(s) 142
Bst4CI ACNGT 3 cut(s) 217, 255, 506
BstDEI CTNAG 5 cut(s) 71, 248, 396, 587, 621
BstF5I GGATG 2 cut(s) 85, 343
BstKTI GATC 8 cut(s) 163, 237, 267, 330, 362, 422, 464, 608
BstMAI GTCTC 3 cut(s) 22, 46, 244
BstMBI GATC 8 cut(s) 160, 234, 264, 327, 359, 419, 461, 605
BstMWI GCNNNNNNNGC 4 cut(s) 132, 229, 446, 558
BstNI CCWGG 1 cut(s) 142
BstNSI RCATGY 1 cut(s) 121
BstSCI CCNGG 2 cut(s) 101, 140
BstSFI CTRYAG 2 cut(s) 190, 216
BstSLI GKGCMC 1 cut(s) 351
BstV1I GCAGC 1 cut(s) 110
BstV2I GAAGAC 2 cut(s) 516, 536
BstX2I RGATCY 3 cut(s) 234, 419, 461
BstYI RGATCY 3 cut(s) 234, 419, 461
Bsu36I CCTNAGG 1 cut(s) 396
BsuRI GGCC 1 cut(s) 272
BtsCI GGATG 2 cut(s) 85, 343
BtsIMutI CAGTG 1 cut(s) 511
Cfr13I GGNCC 1 cut(s) 207
CsiI ACCWGGT 1 cut(s) 140
Csp6I GTAC 3 cut(s) 138, 256, 381
CviAII CATG 4 cut(s) 118, 352, 499, 526
CviQI GTAC 3 cut(s) 138, 256, 381
DdeI CTNAG 5 cut(s) 71, 248, 396, 587, 621
DpnI GATC 8 cut(s) 162, 236, 266, 329, 361, 421, 463, 607
DpnII GATC 8 cut(s) 160, 234, 264, 327, 359, 419, 461, 605
Ecl136II GAGCTC 1 cut(s) 586
Eco130I CCWWGG 1 cut(s) 23
Eco24I GRGCYC 1 cut(s) 588
Eco31I GGTCTC 2 cut(s) 22, 46
Eco47I GGWCC 1 cut(s) 207
Eco53kI GAGCTC 1 cut(s) 586
Eco57I CTGAAG 1 cut(s) 429
Eco81I CCTNAGG 1 cut(s) 396
Eco88I CYCGRG 1 cut(s) 309
EcoICRI GAGCTC 1 cut(s) 586
EcoO109I RGGNCCY 1 cut(s) 207
EcoRII CCWGG 1 cut(s) 140
EcoT14I CCWWGG 1 cut(s) 23
EcoT38I GRGCYC 1 cut(s) 588
ErhI CCWWGG 1 cut(s) 23
Esp3I CGTCTC 1 cut(s) 244
FaeI CATG 4 cut(s) 121, 355, 502, 529
FaiI YATR 7 cut(s) 119, 192, 353, 467, 500, 527, 558
FalI AAGNNNNNCTT 2 cut(s) 266, 298
FaqI GGGAC 1 cut(s) 193
FatI CATG 4 cut(s) 117, 351, 498, 525
Fnu4HI GCNGC 2 cut(s) 99, 553
FokI GGATG 2 cut(s) 92, 350
FriOI GRGCYC 1 cut(s) 588
Fsp4HI GCNGC 2 cut(s) 99, 553
GluI GCNGC 2 cut(s) 99, 553
GsaI CCCAGC 3 cut(s) 19, 43, 67
GsuI CTGGAG 1 cut(s) 141
HaeIII GGCC 1 cut(s) 272
HapII CCGG 2 cut(s) 102, 603
Hin1II CATG 4 cut(s) 121, 355, 502, 529
HincII GTYRAC 1 cut(s) 613
HindII GTYRAC 1 cut(s) 613
HinfI GANTC 2 cut(s) 148, 171
HpaI GTTAAC 1 cut(s) 613
HpaII CCGG 2 cut(s) 102, 603
Hpy166II GTNNAC 2 cut(s) 147, 613
Hpy188I TCNGA 5 cut(s) 132, 165, 337, 419, 541
Hpy188III TCNNGA 3 cut(s) 158, 311, 603
Hpy8I GTNNAC 2 cut(s) 147, 613
HpyAV CCTTC 1 cut(s) 494
HpyCH4III ACNGT 3 cut(s) 217, 255, 506
HpyCH4V TGCA 4 cut(s) 126, 355, 428, 502
HpyF10VI GCNNNNNNNGC 4 cut(s) 132, 229, 446, 558
HpyF3I CTNAG 5 cut(s) 71, 248, 396, 587, 621
Hsp92II CATG 4 cut(s) 121, 355, 502, 529
Kpn2I TCCGGA 1 cut(s) 602
KpnI GGTACC 1 cut(s) 384
KspAI GTTAAC 1 cut(s) 613
Kzo9I GATC 8 cut(s) 160, 234, 264, 327, 359, 419, 461, 605
LmnI GCTCC 1 cut(s) 132
Lsp1109I GCAGC 1 cut(s) 110
LweI GCATC 1 cut(s) 437
MabI ACCWGGT 1 cut(s) 140
MalI GATC 8 cut(s) 162, 236, 266, 329, 361, 421, 463, 607
MboI GATC 8 cut(s) 160, 234, 264, 327, 359, 419, 461, 605
MboII GAAGA 6 cut(s) 334, 337, 414, 435, 521, 541
MfeI CAATTG 1 cut(s) 385
MflI RGATCY 3 cut(s) 234, 419, 461
MhlI GDGCHC 2 cut(s) 351, 588
MluCI AATT 1 cut(s) 385
MlyI GAGTC 1 cut(s) 142
MmeI TCCRAC 1 cut(s) 110
MnlI CCTC 8 cut(s) 77, 185, 262, 391, 535, 539, 602, 610
MroI TCCGGA 1 cut(s) 602
MroXI GAANNNNTTC 1 cut(s) 483
MseI TTAA 2 cut(s) 411, 612
MspA1I CMGCKG 3 cut(s) 232, 247, 449
MspI CCGG 2 cut(s) 102, 603
MspR9I CCNGG 2 cut(s) 103, 142
MunI CAATTG 1 cut(s) 385
MvaI CCWGG 1 cut(s) 142
MwoI GCNNNNNNNGC 4 cut(s) 132, 229, 446, 558
NciI CCSGG 1 cut(s) 103
NdeII GATC 8 cut(s) 160, 234, 264, 327, 359, 419, 461, 605
NlaIII CATG 4 cut(s) 121, 355, 502, 529
NlaIV GGNNCC 5 cut(s) 6, 209, 293, 382, 463
NspI RCATGY 1 cut(s) 121
PcsI WCGNNNNNNNCGW 1 cut(s) 264
PdmI GAANNNNTTC 1 cut(s) 483
PfeI GAWTC 1 cut(s) 171
PkrI GCNGC 2 cut(s) 100, 554
PleI GAGTC 1 cut(s) 142
PpsI GAGTC 1 cut(s) 142
PpuMI RGGWCCY 1 cut(s) 207
Psp124BI GAGCTC 1 cut(s) 588
Psp5II RGGWCCY 1 cut(s) 207
Psp6I CCWGG 1 cut(s) 140
PspFI CCCAGC 3 cut(s) 15, 39, 63
PspGI CCWGG 1 cut(s) 140
PspN4I GGNNCC 5 cut(s) 6, 209, 293, 382, 463
PspPI GGNCC 1 cut(s) 207
PspPPI RGGWCCY 1 cut(s) 207
PsrI GAACNNNNNNTAC 2 cut(s) 210, 242
PsuI RGATCY 3 cut(s) 234, 419, 461
PvuII CAGCTG 2 cut(s) 247, 449
RsaI GTAC 3 cut(s) 139, 257, 382
RsaNI GTAC 3 cut(s) 138, 256, 381
SacI GAGCTC 1 cut(s) 588
SaqAI TTAA 2 cut(s) 411, 612
SatI GCNGC 2 cut(s) 99, 553
Sau3AI GATC 8 cut(s) 160, 234, 264, 327, 359, 419, 461, 605
Sau96I GGNCC 1 cut(s) 207
SchI GAGTC 1 cut(s) 142
ScrFI CCNGG 2 cut(s) 103, 142
SduI GDGCHC 2 cut(s) 351, 588
SetI ASST 8 cut(s) 146, 186, 209, 249, 397, 451, 550, 588
SexAI ACCWGGT 1 cut(s) 140
SfaNI GCATC 1 cut(s) 437
SfcI CTRYAG 2 cut(s) 190, 216
SinI GGWCC 1 cut(s) 207
SmlI CTYRAG 1 cut(s) 581
SmoI CTYRAG 1 cut(s) 581
Sse9I AATT 1 cut(s) 385
SsiI CCGC 3 cut(s) 232, 552, 561
SstI GAGCTC 1 cut(s) 588
StyD4I CCNGG 2 cut(s) 101, 140
StyI CCWWGG 1 cut(s) 23
TaaI ACNGT 3 cut(s) 217, 255, 506
TaqI TCGA 1 cut(s) 267
TasI AATT 1 cut(s) 385
TauI GCSGC 1 cut(s) 555
TfiI GAWTC 1 cut(s) 171
Tru1I TTAA 2 cut(s) 411, 612
Tru9I TTAA 2 cut(s) 411, 612
TscAI CASTG 1 cut(s) 511
TseI GCWGC 1 cut(s) 98
TspDTI ATGAA 1 cut(s) 542
TspGWI ACGGA 2 cut(s) 273, 334
TspRI CASTG 1 cut(s) 511
VpaK11BI GGWCC 1 cut(s) 207
XceI RCATGY 1 cut(s) 121
XmnI GAANNNNTTC 1 cut(s) 483
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.