pycom03g02080

Zinc finger A20 and AN1 domain-containing stress-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0000730
Physical Location & Seq
Reverse (-)
1571874 .. 1572392
519 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g02080.1

Sequence Viewer

Length: 519 bp
ATGGAGCACGAGGAGACTGGATGTCAAGCTGCCCCTGAAGGTCCTATTTTGTGTGTAAACAATTGTGGGTTTTTCGGAAGCGCGGCTACTATGAATATGTGTTCCAAGTGTCACAAGGATATGATGTTGAAACAGGAGCAGGCGAAGCTTGCTGCATCATCCTTTGGAAGCATAGTCAATGGAACATCAAGCATCAGTGCAAATGAGCCTGTTGTTGCGGCTGCTACTGTGGATGTTCAACCCCATCCAGTGGAGCCAAAAACTCTCTCTTTGCAACCATCATTTTCCTTTGGTTCAGGGTCGTCTGGTGAGCCAAAGCCGGAGGGCCCAAAACGTTGCAACACTTGCAACAAGCGGGTTGGATTAACAGGGTTCAGTTGTCGGTGTGGTCACCTTTTTTGTGCAGTACATCGTTATTCAGACAAACATGACTGCCCTTATGATTATCGCACTGCTGGACGCGATGCTATTGCGAAAGCCAACCCTGTCGTAAAAGCTGAGAAGCTCGATAAAATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-A20 zf-AN1

Protein Analysis

173

Amino Acids

18.34

Weight (kDa)

8.0

Isoelectric Point (pI)

35.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-A20 PF01754 16 - 39 1.5e-12 A20-like zinc finger
zf-AN1 PF01428 113 - 149 9.5e-09 AN1-like Zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 250
AccII CGCG 2 cut(s) 83, 462
AciI CCGC 3 cut(s) 83, 218, 355
AclI AACGTT 1 cut(s) 334
AcuI CTGAAG 1 cut(s) 57
AfaI GTAC 1 cut(s) 408
AfiI CCNNNNNNNGG 1 cut(s) 250
AgsI TTSAA 2 cut(s) 130, 239
AhdI GACNNNNNGTC 1 cut(s) 21
AluBI AGCT 4 cut(s) 29, 148, 497, 505
AluI AGCT 4 cut(s) 29, 148, 497, 505
Alw21I GWGCWC 1 cut(s) 9
Alw26I GTCTC 1 cut(s) 8
AoxI GGCC 1 cut(s) 325
ApaI GGGCCC 1 cut(s) 329
ApeKI GCWGC 3 cut(s) 29, 152, 221
AspLEI GCGC 1 cut(s) 83
AspS9I GGNCC 3 cut(s) 41, 325, 326
AsuHPI GGTGA 2 cut(s) 320, 383
AvaII GGWCC 1 cut(s) 41
BaeGI GKGCMC 1 cut(s) 329
BanII GRGCYC 1 cut(s) 329
BarI GAAGNNNNNNTAC 2 cut(s) 70, 102
BauI CACGAG 1 cut(s) 8
Bbv12I GWGCWC 1 cut(s) 9
BbvI GCAGC 3 cut(s) 16, 139, 208
BccI CCATC 2 cut(s) 252, 286
BcgI CGANNNNNNTGC 2 cut(s) 452, 486
BcoDI GTCTC 1 cut(s) 8
BisI GCNGC 5 cut(s) 30, 84, 153, 219, 222
BlsI GCNGC 5 cut(s) 31, 85, 154, 220, 223
Bme18I GGWCC 1 cut(s) 41
BmeRI GACNNNNNGTC 1 cut(s) 21
BmgT120I GGNCC 3 cut(s) 41, 325, 326
BmiI GGNNCC 2 cut(s) 255, 327
BmsI GCATC 3 cut(s) 164, 201, 454
Bsc4I CCNNNNNNNGG 1 cut(s) 250
Bse1I ACTGG 2 cut(s) 22, 248
BseGI GGATG 4 cut(s) 26, 158, 238, 244
BseLI CCNNNNNNNGG 1 cut(s) 250
BseMII CTCAG 1 cut(s) 489
BseNI ACTGG 2 cut(s) 22, 248
BseRI GAGGAG 1 cut(s) 26
BseSI GKGCMC 1 cut(s) 329
BseXI GCAGC 3 cut(s) 16, 139, 208
BsgI GTGCAG 1 cut(s) 423
Bsh1236I CGCG 2 cut(s) 83, 462
BshFI GGCC 1 cut(s) 327
BsiHKAI GWGCWC 1 cut(s) 9
BsiSI CCGG 1 cut(s) 320
BslI CCNNNNNNNGG 1 cut(s) 250
BsmAI GTCTC 1 cut(s) 8
BsnI GGCC 1 cut(s) 327
Bsp120I GGGCCC 1 cut(s) 325
Bsp1286I GDGCHC 2 cut(s) 9, 329
BspACI CCGC 3 cut(s) 83, 218, 355
BspANI GGCC 1 cut(s) 327
BspCNI CTCAG 1 cut(s) 490
BspFNI CGCG 2 cut(s) 83, 462
BspLI GGNNCC 2 cut(s) 255, 327
BsrI ACTGG 2 cut(s) 22, 248
BssSI CACGAG 1 cut(s) 8
Bst2BI CACGAG 1 cut(s) 8
Bst4CI ACNGT 1 cut(s) 229
BstAPI GCANNNNNTGC 1 cut(s) 345
BstC8I GCNNGC 2 cut(s) 141, 150
BstDEI CTNAG 1 cut(s) 498
BstEII GGTNACC 1 cut(s) 389
BstF5I GGATG 4 cut(s) 26, 158, 238, 244
BstFNI CGCG 2 cut(s) 83, 462
BstHHI GCGC 1 cut(s) 83
BstMAI GTCTC 1 cut(s) 8
BstMWI GCNNNNNNNGC 3 cut(s) 145, 149, 345
BstPI GGTNACC 1 cut(s) 389
BstSLI GKGCMC 1 cut(s) 329
BstUI CGCG 2 cut(s) 83, 462
BstV1I GCAGC 3 cut(s) 16, 139, 208
BsuRI GGCC 1 cut(s) 327
BtgZI GCGATG 1 cut(s) 477
BtsCI GGATG 4 cut(s) 26, 158, 238, 244
BtsI GCAGTG 1 cut(s) 450
BtsIMutI CAGTG 3 cut(s) 202, 255, 450
Cac8I GCNNGC 2 cut(s) 141, 150
CfoI GCGC 1 cut(s) 83
Cfr13I GGNCC 3 cut(s) 41, 325, 326
CseI GACGC 1 cut(s) 468
Csp6I GTAC 1 cut(s) 407
CviAII CATG 1 cut(s) 428
CviQI GTAC 1 cut(s) 407
DdeI CTNAG 1 cut(s) 498
DriI GACNNNNNGTC 1 cut(s) 21
Eam1105I GACNNNNNGTC 1 cut(s) 21
Eco24I GRGCYC 1 cut(s) 329
Eco47I GGWCC 1 cut(s) 41
Eco57I CTGAAG 1 cut(s) 57
Eco91I GGTNACC 1 cut(s) 389
EcoO109I RGGNCCY 2 cut(s) 41, 325
EcoO65I GGTNACC 1 cut(s) 389
EcoT38I GRGCYC 1 cut(s) 329
FaeI CATG 1 cut(s) 431
FaiI YATR 6 cut(s) 92, 98, 122, 173, 429, 441
FatI CATG 1 cut(s) 427
FauI CCCGC 1 cut(s) 348
Fnu4HI GCNGC 5 cut(s) 30, 84, 153, 219, 222
FokI GGATG 4 cut(s) 33, 145, 231, 245
FriOI GRGCYC 1 cut(s) 329
Fsp4HI GCNGC 5 cut(s) 30, 84, 153, 219, 222
GlaI GCGC 1 cut(s) 82
GluI GCNGC 5 cut(s) 30, 84, 153, 219, 222
HaeIII GGCC 1 cut(s) 327
HapII CCGG 1 cut(s) 320
HgaI GACGC 1 cut(s) 468
HhaI GCGC 1 cut(s) 83
Hin1II CATG 1 cut(s) 431
Hin6I GCGC 1 cut(s) 81
HinP1I GCGC 1 cut(s) 81
HindIII AAGCTT 1 cut(s) 146
HpaII CCGG 1 cut(s) 320
HphI GGTGA 2 cut(s) 320, 383
Hpy166II GTNNAC 1 cut(s) 58
Hpy188I TCNGA 2 cut(s) 77, 421
Hpy8I GTNNAC 1 cut(s) 58
HpyAV CCTTC 1 cut(s) 32
HpyCH4III ACNGT 1 cut(s) 229
HpyCH4IV ACGT 1 cut(s) 334
HpyCH4V TGCA 6 cut(s) 155, 200, 274, 339, 348, 404
HpyF10VI GCNNNNNNNGC 3 cut(s) 145, 149, 345
HpyF3I CTNAG 1 cut(s) 498
HpySE526I ACGT 1 cut(s) 334
Hsp92II CATG 1 cut(s) 431
HspAI GCGC 1 cut(s) 81
LmnI GCTCC 3 cut(s) 4, 136, 253
Lsp1109I GCAGC 3 cut(s) 16, 139, 208
LweI GCATC 3 cut(s) 164, 201, 454
MaeII ACGT 1 cut(s) 334
MaeIII GTNAC 2 cut(s) 110, 389
MfeI CAATTG 1 cut(s) 61
MhlI GDGCHC 2 cut(s) 9, 329
MluCI AATT 1 cut(s) 61
MmeI TCCRAC 1 cut(s) 340
MnlI CCTC 2 cut(s) 4, 316
MseI TTAA 1 cut(s) 365
MspI CCGG 1 cut(s) 320
MunI CAATTG 1 cut(s) 61
MvnI CGCG 2 cut(s) 83, 462
MwoI GCNNNNNNNGC 3 cut(s) 145, 149, 345
NlaIII CATG 1 cut(s) 431
NlaIV GGNNCC 2 cut(s) 255, 327
NmuCI GTSAC 2 cut(s) 110, 389
PflMI CCANNNNNTGG 1 cut(s) 250
PkrI GCNGC 5 cut(s) 31, 85, 154, 220, 223
PpuMI RGGWCCY 1 cut(s) 41
Psp1406I AACGTT 1 cut(s) 334
Psp5II RGGWCCY 1 cut(s) 41
PspEI GGTNACC 1 cut(s) 389
PspN4I GGNNCC 2 cut(s) 255, 327
PspOMI GGGCCC 1 cut(s) 325
PspPI GGNCC 3 cut(s) 41, 325, 326
PspPPI RGGWCCY 1 cut(s) 41
RsaI GTAC 1 cut(s) 408
RsaNI GTAC 1 cut(s) 407
SaqAI TTAA 1 cut(s) 365
SatI GCNGC 5 cut(s) 30, 84, 153, 219, 222
Sau96I GGNCC 3 cut(s) 41, 325, 326
SduI GDGCHC 2 cut(s) 9, 329
SetI ASST 7 cut(s) 31, 43, 150, 337, 396, 499, 507
SfaNI GCATC 3 cut(s) 164, 201, 454
SinI GGWCC 1 cut(s) 41
Sse9I AATT 1 cut(s) 61
SsiI CCGC 3 cut(s) 83, 218, 355
TaaI ACNGT 1 cut(s) 229
TaiI ACGT 1 cut(s) 337
TaqI TCGA 1 cut(s) 507
TasI AATT 1 cut(s) 61
TatI WGTACW 1 cut(s) 406
TauI GCSGC 2 cut(s) 86, 221
Tru1I TTAA 1 cut(s) 365
Tru9I TTAA 1 cut(s) 365
TscAI CASTG 3 cut(s) 202, 255, 457
TseFI GTSAC 2 cut(s) 110, 389
TseI GCWGC 3 cut(s) 29, 152, 221
Tsp45I GTSAC 2 cut(s) 110, 389
TspDTI ATGAA 1 cut(s) 107
TspRI CASTG 3 cut(s) 202, 255, 457
Van91I CCANNNNNTGG 1 cut(s) 250
VpaK11BI GGWCC 1 cut(s) 41
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.