Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0001011

Physical Location & Seq

Genomic Coordinates Reverse (-)

32995608 .. 33000462

Gene Structure 4855 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1318900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1617 bp

ATGTCGGCGCCGTCTCCGGCTACCAACCCCACGTCGCCTCCACCTTTGTCATCTGCGACTAACACCACTTCACCTCCTCCGACGACGACCAGCGGAACCACACCTTCGCCGCCTCCGCCTGCGTCTAAACCGCCGGGATTGCCGTCGAACCCGAACGTGGGGGTTATAGTAGGGGTTGCAATTGGAGGGTTTTGTACGCTACTTGTGGTGGGCATTTTTTTCCTTTTCTACAGAAGGCGGAAGAGGCGGCAGGAGAGAGCGCGGGAGTCCGGGAACTATCCAGAAGAACCCAAAAATGATTTTGGCGGTCCTCCTCAACATTTGCAGCATAATTATGATCCTCCACCATCAGAAAAAGTAGAACTGTTGCCAAAGCCTACTCATCCCCCAGTACCATCTTCGACTAGTAGCAGCTTGGGCTCTGAGAAACCACCCTCAGTTGCATCGTCTGGCTTTGCCTTTGGGTCGTCACAAATCACATTTACATATGAAGAATTAGCAATGGCAACAAATGGTTTCTCCAATGTCAATCTCCTTGGTCAAGGAGGATTTGGTTTTGTTCATAAAGGAGTCCTACCAAATGGGAAAGTGGTTGCAATTAAGCAGCTGAAAGCTGGGAGTGGGCAAGGGGAGCGTGAATTTCAAGCAGAGATTGAAGTTATTAGTCGCGTCCATCACAGACATCTTGTTTCATTGGTTGGACACTGCATTAGTGGGGCCCAGAGGTTGCTTGTTTACGAGTTCGTTCCAAACGATACCTTGCAATTTCATCTGCATGGAATGGGGAGACCGACTATGGATTGGCAAACTAGATTGAAAATCGCCCTAGGTTCTGCCAAAGGATTGGCGTATCTCCACGAGGACTGTCAACCGAAGATCATACACCGTGACATCAAGGCAGCTAATATTCTTCTTGATCATCACTTTGAGGCAAAGGTTGCAGATTTTGGACTTGCCAAGTTGGCTTTAGATACCGATACTCATGTTTCCACTAGGGTCATGGGAACTTTTGGTTACTTAGCTCCTGAATACGCATCTAGTGGAAAGCTCACTGAGAAGTCAGATGTATTTTCATTTGGGGTTGTGCTGTTGGAGTTGATAACTGGACGCCAACCCATCGATAAAACTCAGACCTTCACTGATGATAGCATGGTTGAGTGGGCGAGGCCTTTGCTTGCGCAAGCATTGGAAACAGGCAACTTTGATGCTCTTGTTGACGTAAGGTTGCAGAATGACTACAACACTAGTGAAATGGCCTGTATGCTTTCTTGCGCTGCTGCTTGCGTGCGTCATTCTGCCCGACGTCGGCCGCGAATGAGCCAGGTGGTTCGAGCCTTGGAAGGAAATCTTTCTGCAGATGAGTTAAATGAGGGAATCATACCAGGTCAAAGTACAGTATTCAGTTACGGAAGCTCAGAGTACAACGCTAGTGAGTACAAGGAAGACTTGAATAAATTCAGAAAACTAGCACTAGCACTAGAAATCCAAGAGCAAGAGACCAGTGAGAACAGTGGACCTAGCAGTGACTTTGGTATGCTCTCAAGTGGTGAACGCCAACAAACCACCCAAGAGATCGAACTTGGGAAAACAAAGAAAAACACCCAAGATCTTGAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009611 GO:0009620 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0023052 GO:0036211 GO:0038023 GO:0043170 GO:0043207 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0046777 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0060089 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04621 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05164 map04010 map04064 map04620 map04621 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05164

Pfam Domains

Protein Families

Pkinase_Tyr

Protein Analysis

539

Amino Acids

58.44

Weight (kDa)

6.26

Isoelectric Point (pI)

51.3

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	174 - 420	2.7e-43	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	177 - 446	1.2e-45	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001011)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G24540 AT3G24550
fragaria_vesca	FvH4_1g04680 FvH4_1g04680 FvH4_6g31540 FvH4_6g31540 FvH4_6g31540 FvH4_6g31540 FvH4_6g31540 FvH4_6g31540
malus_domestica	MD02G1045800.v1.1 MD15G1184000.v1.1 MD15G1318900.v1.1 MD17G1193400.v1.1
prunus_persica	Prupe.3G068800_v2.0.a1 Prupe.7G232200_v2.0.a1
pyrus_communis	pycom02g03820 pycom09g12790 pycom15g16560 pycom17g19940
rosa_chinensis	RchiOBHm_Chr2g0090291 RchiOBHm_Chr2g0138371 RchiOBHm_Chr2g0138381 RchiOBHm_Chr2g0138481 RchiOBHm_Chr2g0138511 RchiOBHm_Chr2g0138521 RchiOBHm_Chr2g0138601 RchiOBHm_Chr6g0297471
rosa_laevigata	RLG00000016095 RLG00000019740 RLG00000019744 RLG00000019747
rosa_multiflora	Rmu_co8232347.1_g000001 Rmu_co8362561.1_g000001 Rmu_sc0000436.1_g000004 Rmu_sc0001534.1_g000011 Rmu_sc0006235.1_g000002 Rmu_sc0008467.1_g000011 Rmu_sc0012206.1_g000004
rosa_roxburghii	Rroxscaffold_2G00106070 Rroxscaffold_2G00106140 Rroxscaffold_2G00106150 Rroxscaffold_2G00106280 Rroxscaffold_2G00151310
rosa_rugosa	Rorug02G0006300 Rorug02G0351700 Rorug06G0272400
rosa_samantha	Rh2AG052200 Rh2AG400100 Rh2AG400200 Rh2AG400800 Rh2CG386300 Rh2DG052100 Rh2DG419800 Rh2DG419900 Rh2DG420100 Rh2DG420600 Rh2DG421200
rosa_wichuraiana	Rw2G004470 Rw2G032550 Rw2G032580

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	1306
Acc16I	TGCGCA	1 cut(s)	1179
AccB1I	GGYRCC	1 cut(s)	7
AccII	CGCG	3 cut(s)	262, 669, 1312
AciI	CCGC	9 cut(s)	93, 110, 116, 131, 238, 247, 262, 306, 1310
AclWI	GGATC	1 cut(s)	332
AcoI	YGGCCR	1 cut(s)	1307
AcsI	RAATTY	2 cut(s)	638, 1454
AcyI	GRCGYC	3 cut(s)	8, 1108, 1303
AfaI	GTAC	5 cut(s)	196, 393, 1393, 1421, 1436
AfiI	CCNNNNNNNGG	3 cut(s)	157, 158, 1305
AgsI	TTSAA	4 cut(s)	644, 656, 817, 1450
AhlI	ACTAGT	2 cut(s)	404, 1244
AjiI	CACGTC	1 cut(s)	33
AjnI	CCWGG	2 cut(s)	1320, 1381
AluBI	AGCT	7 cut(s)	414, 607, 614, 902, 1022, 1048, 1413
AluI	AGCT	7 cut(s)	414, 607, 614, 902, 1022, 1048, 1413
Alw26I	GTCTC	3 cut(s)	18, 781, 1490
AlwI	GGATC	1 cut(s)	332
AoxI	GGCC	4 cut(s)	717, 1166, 1254, 1307
ApaI	GGGCCC	1 cut(s)	721
ApeKI	GCWGC	6 cut(s)	325, 411, 604, 899, 1274, 1277
ApoI	RAATTY	2 cut(s)	638, 1454
Asp700I	GAANNNNTTC	2 cut(s)	1348, 1454
AspA2I	CCTAGG	1 cut(s)	826
AspLEI	GCGC	4 cut(s)	10, 262, 1180, 1274
AspS9I	GGNCC	4 cut(s)	308, 717, 718, 1514
AsuC2I	CCSGG	2 cut(s)	135, 271
AsuHPI	GGTGA	2 cut(s)	63, 1559
AvaII	GGWCC	2 cut(s)	308, 1514
AvrII	CCTAGG	1 cut(s)	826
BaeGI	GKGCMC	1 cut(s)	721
BaeI	ACNNNNGTAYC	2 cut(s)	969, 1002
BanI	GGYRCC	1 cut(s)	7
BanII	GRGCYC	2 cut(s)	422, 721
BauI	CACGAG	1 cut(s)	857
BbsI	GAAGAC	1 cut(s)	1449
BbvI	GCAGC	6 cut(s)	337, 423, 616, 911, 1261, 1264
BccI	CCATC	4 cut(s)	355, 403, 681, 1124
BceAI	ACGGC	1 cut(s)	127
BcgI	CGANNNNNNTGC	2 cut(s)	1153, 1187
BciT130I	CCWGG	2 cut(s)	1322, 1383
BclI	TGATCA	1 cut(s)	916
BcnI	CCSGG	2 cut(s)	135, 271
BcoDI	GTCTC	3 cut(s)	18, 781, 1490
BcuI	ACTAGT	2 cut(s)	404, 1244
BfmI	CTRYAG	2 cut(s)	229, 1353
BfoI	RGCGCY	1 cut(s)	11
BglI	GCCNNNNNGGC	1 cut(s)	962
BglII	AGATCT	1 cut(s)	1606
BisI	GCNGC	9 cut(s)	110, 248, 326, 412, 605, 900, 1275, 1278, 1310
BlnI	CCTAGG	1 cut(s)	826
BlsI	GCNGC	9 cut(s)	111, 249, 327, 413, 606, 901, 1276, 1279, 1311
Bme1390I	CCNGG	4 cut(s)	135, 271, 1322, 1383
Bme18I	GGWCC	2 cut(s)	308, 1514
BmgBI	CACGTC	1 cut(s)	33
BmgT120I	GGNCC	4 cut(s)	308, 717, 718, 1514
BmiI	GGNNCC	4 cut(s)	9, 97, 718, 719
BmrFI	CCNGG	4 cut(s)	135, 271, 1322, 1383
BmrI	ACTGGG	1 cut(s)	383
BmsI	GCATC	3 cut(s)	452, 1043, 1195
BmuI	ACTGGG	1 cut(s)	383
BpiI	GAAGAC	1 cut(s)	1449
BpuEI	CTTGAG	1 cut(s)	1525
BpuMI	CCSGG	2 cut(s)	135, 271
Bsa29I	ATCGAT	1 cut(s)	1119
BsaHI	GRCGYC	3 cut(s)	8, 1108, 1303
BsaI	GGTCTC	2 cut(s)	781, 1490
BsaJI	CCNNGG	3 cut(s)	535, 826, 1335
BsaXI	ACNNNNNCTCC	4 cut(s)	22, 52, 58, 88
Bsc4I	CCNNNNNNNGG	3 cut(s)	157, 158, 1305
Bse1I	ACTGG	3 cut(s)	389, 1108, 1500
Bse3DI	GCAATG	1 cut(s)	507
BseBI	CCWGG	2 cut(s)	1322, 1383
BseCI	ATCGAT	1 cut(s)	1119
BseDI	CCNNGG	3 cut(s)	535, 826, 1335
BseGI	GGATG	1 cut(s)	382
BseLI	CCNNNNNNNGG	3 cut(s)	157, 158, 1305
BseMI	GCAATG	1 cut(s)	507
BseMII	CTCAG	5 cut(s)	414, 450, 1044, 1142, 1428
BseNI	ACTGG	3 cut(s)	389, 1108, 1500
BseRI	GAGGAG	2 cut(s)	66, 303
BseSI	GKGCMC	1 cut(s)	721
BseX3I	CGGCCG	1 cut(s)	1307
BseXI	GCAGC	6 cut(s)	337, 423, 616, 911, 1261, 1264
BseYI	CCCAGC	1 cut(s)	614
Bsh1236I	CGCG	3 cut(s)	262, 669, 1312
Bsh1285I	CGRYCG	1 cut(s)	1310
BshFI	GGCC	4 cut(s)	719, 1168, 1256, 1309
BshNI	GGYRCC	1 cut(s)	7
BshVI	ATCGAT	1 cut(s)	1119
BsiEI	CGRYCG	1 cut(s)	1310
BsiSI	CCGG	3 cut(s)	17, 134, 270
BslI	CCNNNNNNNGG	3 cut(s)	157, 158, 1305
BsmAI	GTCTC	3 cut(s)	18, 781, 1490
BsmBI	CGTCTC	1 cut(s)	18
BsnI	GGCC	4 cut(s)	719, 1168, 1256, 1309
Bso31I	GGTCTC	2 cut(s)	781, 1490
Bsp120I	GGGCCC	1 cut(s)	717
Bsp1286I	GDGCHC	2 cut(s)	422, 721
Bsp143I	GATC	5 cut(s)	337, 876, 916, 1572, 1606
BspACI	CCGC	9 cut(s)	93, 110, 116, 131, 238, 247, 262, 306, 1310
BspANI	GGCC	4 cut(s)	719, 1168, 1256, 1309
BspCNI	CTCAG	5 cut(s)	415, 449, 1045, 1141, 1427
BspDI	ATCGAT	1 cut(s)	1119
BspFNI	CGCG	3 cut(s)	262, 669, 1312
BspLI	GGNNCC	4 cut(s)	9, 97, 718, 719
BspMAI	CTGCAG	1 cut(s)	1357
BspPI	GGATC	1 cut(s)	332
BspT107I	GGYRCC	1 cut(s)	7
BspTNI	GGTCTC	2 cut(s)	781, 1490
BsrDI	GCAATG	1 cut(s)	507
BsrI	ACTGG	3 cut(s)	389, 1108, 1500
BssECI	CCNNGG	3 cut(s)	535, 826, 1335
BssMI	GATC	5 cut(s)	337, 876, 916, 1572, 1606
BssNI	GRCGYC	3 cut(s)	8, 1108, 1303
BssSI	CACGAG	1 cut(s)	857
BssT1I	CCWWGG	3 cut(s)	535, 826, 1335
Bst2BI	CACGAG	1 cut(s)	857
Bst2UI	CCWGG	2 cut(s)	1322, 1383
Bst4CI	ACNGT	5 cut(s)	366, 866, 887, 1396, 1511
Bst6I	CTCTTC	1 cut(s)	236
BstACI	GRCGYC	3 cut(s)	8, 1108, 1303
BstAPI	GCANNNNNTGC	1 cut(s)	938
BstC8I	GCNNGC	5 cut(s)	120, 1176, 1182, 1282, 1286
BstDEI	CTNAG	6 cut(s)	423, 436, 1018, 1053, 1128, 1414
BstF5I	GGATG	1 cut(s)	382
BstFNI	CGCG	3 cut(s)	262, 669, 1312
BstH2I	RGCGCY	1 cut(s)	11
BstHHI	GCGC	4 cut(s)	10, 262, 1180, 1274
BstKTI	GATC	5 cut(s)	340, 879, 919, 1575, 1609
BstMAI	GTCTC	3 cut(s)	18, 781, 1490
BstMBI	GATC	5 cut(s)	337, 876, 916, 1572, 1606
BstMCI	CGRYCG	1 cut(s)	1310
BstMWI	GCNNNNNNNGC	7 cut(s)	115, 139, 244, 417, 631, 938, 962
BstNI	CCWGG	2 cut(s)	1322, 1383
BstSCI	CCNGG	4 cut(s)	133, 269, 1320, 1381
BstSFI	CTRYAG	2 cut(s)	229, 1353
BstSLI	GKGCMC	1 cut(s)	721
BstUI	CGCG	3 cut(s)	262, 669, 1312
BstV1I	GCAGC	6 cut(s)	337, 423, 616, 911, 1261, 1264
BstV2I	GAAGAC	1 cut(s)	1449
BstX2I	RGATCY	1 cut(s)	1606
BstXI	CCANNNNNNTGG	1 cut(s)	844
BstYI	RGATCY	1 cut(s)	1606
BstZI	CGGCCG	1 cut(s)	1307
Bsu15I	ATCGAT	1 cut(s)	1119
BsuRI	GGCC	4 cut(s)	719, 1168, 1256, 1309
BsuTUI	ATCGAT	1 cut(s)	1119
BtrI	CACGTC	1 cut(s)	33
BtsCI	GGATG	1 cut(s)	382
BtsI	GCAGTG	2 cut(s)	703, 1528
BtsIMutI	CAGTG	6 cut(s)	703, 1050, 1137, 1507, 1516, 1528
Cac8I	GCNNGC	5 cut(s)	120, 1176, 1182, 1282, 1286
CfoI	GCGC	4 cut(s)	10, 262, 1180, 1274
Cfr13I	GGNCC	4 cut(s)	308, 717, 718, 1514
ClaI	ATCGAT	1 cut(s)	1119
CseI	GACGC	4 cut(s)	111, 658, 1116, 1277
CsiI	ACCWGGT	1 cut(s)	1381
Csp6I	GTAC	5 cut(s)	195, 392, 1392, 1420, 1435
CviAII	CATG	4 cut(s)	776, 983, 1000, 1150
CviQI	GTAC	5 cut(s)	195, 392, 1392, 1420, 1435
DdeI	CTNAG	6 cut(s)	423, 436, 1018, 1053, 1128, 1414
DinI	GGCGCC	1 cut(s)	9
DpnI	GATC	5 cut(s)	339, 878, 918, 1574, 1608
DpnII	GATC	5 cut(s)	337, 876, 916, 1572, 1606
EaeI	YGGCCR	1 cut(s)	1307
EagI	CGGCCG	1 cut(s)	1307
Eam1104I	CTCTTC	1 cut(s)	236
EarI	CTCTTC	1 cut(s)	236
EciI	GGCGGA	2 cut(s)	105, 253
EclXI	CGGCCG	1 cut(s)	1307
Eco130I	CCWWGG	3 cut(s)	535, 826, 1335
Eco147I	AGGCCT	1 cut(s)	1168
Eco24I	GRGCYC	2 cut(s)	422, 721
Eco31I	GGTCTC	2 cut(s)	781, 1490
Eco47I	GGWCC	2 cut(s)	308, 1514
Eco52I	CGGCCG	1 cut(s)	1307
EcoO109I	RGGNCCY	1 cut(s)	717
EcoRII	CCWGG	2 cut(s)	1320, 1381
EcoT14I	CCWWGG	3 cut(s)	535, 826, 1335
EcoT38I	GRGCYC	2 cut(s)	422, 721
EgeI	GGCGCC	1 cut(s)	9
EheI	GGCGCC	1 cut(s)	9
ErhI	CCWWGG	3 cut(s)	535, 826, 1335
Esp3I	CGTCTC	1 cut(s)	18
FaeI	CATG	4 cut(s)	779, 986, 1003, 1153
FalI	AAGNNNNNCTT	4 cut(s)	1332, 1364, 1430, 1462
FatI	CATG	4 cut(s)	775, 982, 999, 1149
FauI	CCCGC	1 cut(s)	255
FauNDI	CATATG	1 cut(s)	487
FbaI	TGATCA	1 cut(s)	916
Fnu4HI	GCNGC	9 cut(s)	110, 248, 326, 412, 605, 900, 1275, 1278, 1310
FokI	GGATG	1 cut(s)	369
FriOI	GRGCYC	2 cut(s)	422, 721
Fsp4HI	GCNGC	9 cut(s)	110, 248, 326, 412, 605, 900, 1275, 1278, 1310
FspI	TGCGCA	1 cut(s)	1179
GlaI	GCGC	4 cut(s)	9, 261, 1179, 1273
GluI	GCNGC	9 cut(s)	110, 248, 326, 412, 605, 900, 1275, 1278, 1310
GsaI	CCCAGC	1 cut(s)	618
HaeII	RGCGCY	1 cut(s)	11
HaeIII	GGCC	4 cut(s)	719, 1168, 1256, 1309
HapII	CCGG	3 cut(s)	17, 134, 270
HgaI	GACGC	4 cut(s)	111, 658, 1116, 1277
HhaI	GCGC	4 cut(s)	10, 262, 1180, 1274
Hin1I	GRCGYC	3 cut(s)	8, 1108, 1303
Hin1II	CATG	4 cut(s)	779, 986, 1003, 1153
Hin6I	GCGC	4 cut(s)	8, 260, 1178, 1272
HinP1I	GCGC	4 cut(s)	8, 260, 1178, 1272
HincII	GTYRAC	2 cut(s)	869, 1216
HindII	GTYRAC	2 cut(s)	869, 1216
HinfI	GANTC	3 cut(s)	266, 570, 1374
HpaII	CCGG	3 cut(s)	17, 134, 270
HphI	GGTGA	2 cut(s)	63, 1559
Hpy166II	GTNNAC	5 cut(s)	736, 869, 1216, 1514, 1550
Hpy188I	TCNGA	7 cut(s)	81, 352, 424, 1063, 1131, 1417, 1460
Hpy188III	TCNNGA	4 cut(s)	281, 914, 1025, 1610
Hpy8I	GTNNAC	5 cut(s)	736, 869, 1216, 1514, 1550
Hpy99I	CGWCG	6 cut(s)	37, 85, 88, 148, 1305, 1308
HpyAV	CCTTC	4 cut(s)	114, 228, 1144, 1334
HpyCH4III	ACNGT	5 cut(s)	366, 866, 887, 1396, 1511
HpyCH4IV	ACGT	4 cut(s)	32, 156, 1218, 1303
HpyF10VI	GCNNNNNNNGC	7 cut(s)	115, 139, 244, 417, 631, 938, 962
HpyF3I	CTNAG	6 cut(s)	423, 436, 1018, 1053, 1128, 1414
HpySE526I	ACGT	4 cut(s)	32, 156, 1218, 1303
Hsp92I	GRCGYC	3 cut(s)	8, 1108, 1303
Hsp92II	CATG	4 cut(s)	779, 986, 1003, 1153
HspAI	GCGC	4 cut(s)	8, 260, 1178, 1272
KasI	GGCGCC	1 cut(s)	7
Ksp22I	TGATCA	1 cut(s)	916
Kzo9I	GATC	5 cut(s)	337, 876, 916, 1572, 1606
LmnI	GCTCC	2 cut(s)	631, 1027
Lsp1109I	GCAGC	6 cut(s)	337, 423, 616, 911, 1261, 1264
LweI	GCATC	3 cut(s)	452, 1043, 1195
MabI	ACCWGGT	1 cut(s)	1381
MaeII	ACGT	4 cut(s)	32, 156, 1218, 1303
MaeIII	GTNAC	5 cut(s)	468, 887, 1013, 1403, 1523
MalI	GATC	5 cut(s)	339, 878, 918, 1574, 1608
MboI	GATC	5 cut(s)	337, 876, 916, 1572, 1606
MboII	GAAGA	7 cut(s)	253, 296, 390, 503, 886, 902, 1454
MfeI	CAATTG	1 cut(s)	180
MflI	RGATCY	1 cut(s)	1606
MhlI	GDGCHC	2 cut(s)	422, 721
MluCI	AATT	7 cut(s)	180, 331, 494, 597, 638, 764, 1454
Mly113I	GGCGCC	1 cut(s)	8
MlyI	GAGTC	2 cut(s)	275, 579
MmeI	TCCRAC	3 cut(s)	104, 679, 1071
MroXI	GAANNNNTTC	2 cut(s)	1348, 1454
MseI	TTAA	2 cut(s)	600, 1364
MslI	CAYNNNNRTG	2 cut(s)	333, 774
MspA1I	CMGCKG	2 cut(s)	93, 607
MspI	CCGG	3 cut(s)	17, 134, 270
MspR9I	CCNGG	4 cut(s)	135, 271, 1322, 1383
MunI	CAATTG	1 cut(s)	180
MvaI	CCWGG	2 cut(s)	1322, 1383
MvnI	CGCG	3 cut(s)	262, 669, 1312
MwoI	GCNNNNNNNGC	7 cut(s)	115, 139, 244, 417, 631, 938, 962
NarI	GGCGCC	1 cut(s)	8
NciI	CCSGG	2 cut(s)	135, 271
NdeI	CATATG	1 cut(s)	487
NdeII	GATC	5 cut(s)	337, 876, 916, 1572, 1606
NlaIII	CATG	4 cut(s)	779, 986, 1003, 1153
NlaIV	GGNNCC	4 cut(s)	9, 97, 718, 719
NmuCI	GTSAC	3 cut(s)	468, 887, 1523
NsbI	TGCGCA	1 cut(s)	1179
PceI	AGGCCT	1 cut(s)	1168
PcsI	WCGNNNNNNNCGW	2 cut(s)	750, 1309
PdmI	GAANNNNTTC	2 cut(s)	1348, 1454
PfeI	GAWTC	1 cut(s)	1374
PfoI	TCCNGGA	1 cut(s)	269
PkrI	GCNGC	9 cut(s)	111, 249, 327, 413, 606, 901, 1276, 1279, 1311
PleI	GAGTC	2 cut(s)	274, 578
PluTI	GGCGCC	1 cut(s)	11
PpsI	GAGTC	2 cut(s)	274, 578
Psp6I	CCWGG	2 cut(s)	1320, 1381
PspFI	CCCAGC	1 cut(s)	614
PspGI	CCWGG	2 cut(s)	1320, 1381
PspN4I	GGNNCC	4 cut(s)	9, 97, 718, 719
PspOMI	GGGCCC	1 cut(s)	717
PspPI	GGNCC	4 cut(s)	308, 717, 718, 1514
PstI	CTGCAG	1 cut(s)	1357
PsuI	RGATCY	1 cut(s)	1606
PvuII	CAGCTG	1 cut(s)	607
RsaI	GTAC	5 cut(s)	196, 393, 1393, 1421, 1436
RsaNI	GTAC	5 cut(s)	195, 392, 1392, 1420, 1435
RseI	CAYNNNNRTG	2 cut(s)	333, 774
SaqAI	TTAA	2 cut(s)	600, 1364
SatI	GCNGC	9 cut(s)	110, 248, 326, 412, 605, 900, 1275, 1278, 1310
Sau3AI	GATC	5 cut(s)	337, 876, 916, 1572, 1606
Sau96I	GGNCC	4 cut(s)	308, 717, 718, 1514
SchI	GAGTC	2 cut(s)	275, 579
ScrFI	CCNGG	4 cut(s)	135, 271, 1322, 1383
SduI	GDGCHC	2 cut(s)	422, 721
SexAI	ACCWGGT	1 cut(s)	1381
SfaNI	GCATC	3 cut(s)	452, 1043, 1195
SfcI	CTRYAG	2 cut(s)	229, 1353
SfoI	GGCGCC	1 cut(s)	9
SinI	GGWCC	2 cut(s)	308, 1514
SmiMI	CAYNNNNRTG	2 cut(s)	333, 774
SmlI	CTYRAG	2 cut(s)	1540, 1610
SmoI	CTYRAG	2 cut(s)	1540, 1610
SpeI	ACTAGT	2 cut(s)	404, 1244
Sse9I	AATT	7 cut(s)	180, 331, 494, 597, 638, 764, 1454
SseBI	AGGCCT	1 cut(s)	1168
SsiI	CCGC	9 cut(s)	93, 110, 116, 131, 238, 247, 262, 306, 1310
SspDI	GGCGCC	1 cut(s)	7
SspI	AATATT	1 cut(s)	907
StuI	AGGCCT	1 cut(s)	1168
StyD4I	CCNGG	4 cut(s)	133, 269, 1320, 1381
StyI	CCWWGG	3 cut(s)	535, 826, 1335
TaaI	ACNGT	5 cut(s)	366, 866, 887, 1396, 1511
TaiI	ACGT	4 cut(s)	35, 159, 1221, 1306
TaqI	TCGA	5 cut(s)	146, 401, 1119, 1330, 1575
TaqII	GACCGA	1 cut(s)	805
TasI	AATT	7 cut(s)	180, 331, 494, 597, 638, 764, 1454
TatI	WGTACW	3 cut(s)	1391, 1419, 1434
TauI	GCSGC	3 cut(s)	112, 250, 1312
TfiI	GAWTC	1 cut(s)	1374
Tru1I	TTAA	2 cut(s)	600, 1364
Tru9I	TTAA	2 cut(s)	600, 1364
TscAI	CASTG	6 cut(s)	710, 1057, 1144, 1507, 1516, 1528
TseFI	GTSAC	3 cut(s)	468, 887, 1523
TseI	GCWGC	6 cut(s)	325, 411, 604, 899, 1274, 1277
Tsp45I	GTSAC	3 cut(s)	468, 887, 1523
TspDTI	ATGAA	5 cut(s)	504, 551, 681, 758, 1062
TspGWI	ACGGA	1 cut(s)	1422
TspRI	CASTG	6 cut(s)	710, 1057, 1144, 1507, 1516, 1528
VpaK11BI	GGWCC	2 cut(s)	308, 1514
XapI	RAATTY	2 cut(s)	638, 1454
XcmI	CCANNNNNNNNNTGG	1 cut(s)	997
XmaJI	CCTAGG	1 cut(s)	826
XmnI	GAANNNNTTC	2 cut(s)	1348, 1454
ZraI	GACGTC	1 cut(s)	1304

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.