pycom06g04320

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0001095
Physical Location & Seq
Forward (+)
5465777 .. 5469238
3462 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1617 bp
ATGGCGTGGAATCAGATCAAGTACTCGATGCTGAACCTTCGAAGCTCTGATTTCTCTATCCGTGAAGAATCCATGGATTCCCAGATTCTGAGCTCTCCACGCCCGCTCTCCATCGCTTCCGAGCCTTCAGATCTGAAAAACTGGTTCCCCAGCTACCAATATGAATCACCTGCACTGAAGTCCAATTCTTTTCTCGATGATACCGTTGTCCCCGAAACTGAGATGGAAGAAAACTCAGGTGGGTTCGAGAGAAATGGAACAAAAAATGAAGCTTTTATTCCTAAGAACGAAACCCCAAATGGGGTTGTGAAATGTGGCAGCTTTTCTGGATATGAGCACGAAAGCCATTTGTTAAGTAAGATCCCAAGTTCTTTGGAATCAACTTCGTTCGTTTCAGAGCCCACCGACATCAGAAACTGGTACTCAAGCTATGCATATGAATCGCTATCATTGGATACAATTGATGGTGTTGGAAATTCCATTTGCGAAGTGAGTAAACACGAGGAGGATGGCTTTTTGGTTGAAAACAACAGAGGAAAAGAAAAGGTTTCGGAGGATTTTAGTATAAACAGAAGTGATTCTGAAGAGGTTAGTGGGAGAGCTCCTAAGGAGGAAAGGCAGCCTGTAAGAGAGACTCCAGGTCCTTTAGAGCCGCCTTCGCAACTGTCAGAGCCTACTGACATCAGAAACTGGTTCTCAAGCTATGTGTACGAGTCTCCGTCCTATGATACAAGTACAACTGATGGTTTTCGAGATCCTGTTTGGAAAGAAAACAAAAGTGGGAAGGATGGGTTTCTGGTTGATAATAGCCACAGAGAAAATGAAGAGGGCTTGGAGGGTTATAATGAAAAGGGAACTGATGGCGAAGCGGTTTCTGATTTACAGTCGGAAGGGCTTGCAAAATGCATCACCTCTTGGAGAGATAATAAGCAGAAAACACAGCCTGGTCGCCCTAGCTATGGAGTAAGCGAAAACCTACTTAGTCAGAATGATTTGTGTCTCAAGCATATGCCTAAATCATGGAGAACCAAAGTCATCGCCCAAGCACAAGATGTCGAAATGTCAATCTTGAACAAGGAAAGTTGTCAATGCAAGCTATCGCCTAAGAGTTTCAAGGGAACCAAAATTAAGCCATCGGACAACGAAAAAAGTCCATGCAGGAACTATGAGAAGTCTTCACAAGGCATGACTCCTAGCAAGGCCTTCACTTCACAGGAGAACGCAGAAGCAGAATTCCACACAGAAGTTGATTGTATATTAACTGGAAGTGATTCGGATTTAATTCCAAGCAATGGAGTGTCAGAACGAAAACCAACTCATGGAAGCAGAGACAGAAAAGATGATGGAAATGAAATATCGGAAAATGGTTTTATAACAACTAGGAAAGGCAAACTTACAAGAAACAATCAGGAAAAGATTGTGGAACGACCGCAAGAGATTAGTAAAATGACAGCTCCAATAGTCGTGAAAAGAAAAGCCTTGGCAGATGCAACAAATATTCATCATTCTCCTGCGGCGGGGATTACAGGGAAATGGAAGTGTCCTCAGAAAAGTAAGCCAAATCGAGGACCCGCTTTGAAGCAGCTTCGACTTGAGAAATGGGTTCGTAAGTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

539

Amino Acids

60.31

Weight (kDa)

5.51

Isoelectric Point (pI)

45.99

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 843, 1373
AarI CACCTGC 1 cut(s) 178
Acc36I ACCTGC 1 cut(s) 178
AccB7I CCANNNNNTGG 2 cut(s) 1292, 1319
AccBSI CCGCTC 1 cut(s) 106
AciI CCGC 7 cut(s) 104, 653, 869, 1430, 1514, 1517, 1572
AclWI GGATC 2 cut(s) 355, 749
AcsI RAATTY 2 cut(s) 475, 1232
AcuI CTGAAG 3 cut(s) 111, 197, 603
AfaI GTAC 4 cut(s) 23, 422, 710, 736
AfiI CCNNNNNNNGG 7 cut(s) 300, 301, 959, 1292, 1319, 1517, 1565
AgsI TTSAA 4 cut(s) 524, 1072, 1114, 1579
AhdI GACNNNNNGTC 1 cut(s) 639
AjnI CCWGG 2 cut(s) 637, 943
Alw21I GWGCWC 3 cut(s) 95, 339, 604
Alw26I GTCTC 4 cut(s) 626, 720, 1004, 1323
AlwI GGATC 2 cut(s) 355, 749
AlwNI CAGNNNCTG 3 cut(s) 88, 417, 690
AoxI GGCC 1 cut(s) 1200
ApeKI GCWGC 3 cut(s) 318, 619, 1582
ApoI RAATTY 2 cut(s) 475, 1232
Asp700I GAANNNNTTC 2 cut(s) 577, 1270
AspS9I GGNCC 2 cut(s) 641, 1568
AsuHPI GGTGA 2 cut(s) 159, 901
AsuII TTCGAA 1 cut(s) 40
AvaII GGWCC 2 cut(s) 641, 1568
AxyI CCTNAGG 1 cut(s) 606
BaeI ACNNNNGTAYC 2 cut(s) 192, 225
BanII GRGCYC 3 cut(s) 95, 402, 604
BauI CACGAG 1 cut(s) 500
BbsI GAAGAC 1 cut(s) 1167
Bbv12I GWGCWC 3 cut(s) 95, 339, 604
BbvI GCAGC 3 cut(s) 330, 631, 1594
BccI CCATC 9 cut(s) 119, 217, 458, 503, 737, 782, 854, 1141, 1337
BcgI CGANNNNNNTGC 2 cut(s) 423, 457
BciT130I CCWGG 2 cut(s) 639, 945
BciVI GTATCC 1 cut(s) 448
BcoDI GTCTC 4 cut(s) 626, 720, 1004, 1323
BfaI CTAG 3 cut(s) 954, 1194, 1380
BfuAI ACCTGC 1 cut(s) 178
BfuI GTATCC 1 cut(s) 448
BglII AGATCT 1 cut(s) 130
BisI GCNGC 5 cut(s) 319, 620, 653, 1515, 1583
BlsI GCNGC 5 cut(s) 320, 621, 654, 1516, 1584
BmcAI AGTACT 1 cut(s) 23
Bme1390I CCNGG 2 cut(s) 639, 945
Bme18I GGWCC 2 cut(s) 641, 1568
BmeRI GACNNNNNGTC 1 cut(s) 639
BmgT120I GGNCC 2 cut(s) 641, 1568
BmiI GGNNCC 3 cut(s) 146, 1120, 1570
BmrFI CCNGG 2 cut(s) 639, 945
BmsI GCATC 3 cut(s) 18, 915, 1477
BpiI GAAGAC 1 cut(s) 1167
BpmI CTGGAG 1 cut(s) 621
Bpu14I TTCGAA 1 cut(s) 40
BpuEI CTTGAG 4 cut(s) 409, 682, 986, 1613
BsaJI CCNNGG 2 cut(s) 72, 1479
Bsc4I CCNNNNNNNGG 7 cut(s) 300, 301, 959, 1292, 1319, 1517, 1565
Bse1I ACTGG 4 cut(s) 146, 422, 695, 1267
Bse21I CCTNAGG 1 cut(s) 606
Bse3DI GCAATG 1 cut(s) 1297
BseBI CCWGG 2 cut(s) 639, 945
BseDI CCNNGG 2 cut(s) 72, 1479
BseGI GGATG 2 cut(s) 514, 793
BseLI CCNNNNNNNGG 7 cut(s) 300, 301, 959, 1292, 1319, 1517, 1565
BseMI GCAATG 1 cut(s) 1297
BseMII CTCAG 4 cut(s) 80, 210, 249, 1559
BseNI ACTGG 4 cut(s) 146, 422, 695, 1267
BseRI GAGGAG 1 cut(s) 518
BseXI GCAGC 3 cut(s) 330, 631, 1594
BseYI CCCAGC 1 cut(s) 149
BsgI GTGCAG 1 cut(s) 156
Bsh1285I CGRYCG 1 cut(s) 1430
BshFI GGCC 1 cut(s) 1202
BsiEI CGRYCG 1 cut(s) 1430
BsiHKAI GWGCWC 3 cut(s) 95, 339, 604
BslFI GGGAC 1 cut(s) 194
BslI CCNNNNNNNGG 7 cut(s) 300, 301, 959, 1292, 1319, 1517, 1565
BsmAI GTCTC 4 cut(s) 626, 720, 1004, 1323
BsmFI GGGAC 1 cut(s) 194
BsnI GGCC 1 cut(s) 1202
Bsp119I TTCGAA 1 cut(s) 40
Bsp1286I GDGCHC 4 cut(s) 95, 339, 402, 604
Bsp143I GATC 4 cut(s) 15, 130, 360, 754
Bsp19I CCATGG 1 cut(s) 72
BspACI CCGC 7 cut(s) 104, 653, 869, 1430, 1514, 1517, 1572
BspANI GGCC 1 cut(s) 1202
BspCNI CTCAG 4 cut(s) 81, 211, 248, 1558
BspLI GGNNCC 3 cut(s) 146, 1120, 1570
BspMI ACCTGC 1 cut(s) 178
BspPI GGATC 2 cut(s) 355, 749
BspT104I TTCGAA 1 cut(s) 40
BsrBI CCGCTC 1 cut(s) 106
BsrDI GCAATG 1 cut(s) 1297
BsrI ACTGG 4 cut(s) 146, 422, 695, 1267
BssECI CCNNGG 2 cut(s) 72, 1479
BssMI GATC 4 cut(s) 15, 130, 360, 754
BssSI CACGAG 1 cut(s) 500
BssT1I CCWWGG 2 cut(s) 72, 1479
Bst2BI CACGAG 1 cut(s) 500
Bst2UI CCWGG 2 cut(s) 639, 945
Bst4CI ACNGT 3 cut(s) 205, 666, 885
Bst6I CTCTTC 2 cut(s) 579, 819
BstBI TTCGAA 1 cut(s) 40
BstC8I GCNNGC 3 cut(s) 104, 897, 1094
BstDEI CTNAG 8 cut(s) 89, 219, 235, 282, 606, 980, 1104, 1545
BstDSI CCRYGG 1 cut(s) 72
BstF5I GGATG 2 cut(s) 514, 793
BstKTI GATC 4 cut(s) 18, 133, 363, 757
BstMAI GTCTC 4 cut(s) 626, 720, 1004, 1323
BstMBI GATC 4 cut(s) 15, 130, 360, 754
BstMCI CGRYCG 1 cut(s) 1430
BstMWI GCNNNNNNNGC 2 cut(s) 99, 658
BstNI CCWGG 2 cut(s) 639, 945
BstSCI CCNGG 2 cut(s) 637, 943
BstV1I GCAGC 3 cut(s) 330, 631, 1594
BstV2I GAAGAC 1 cut(s) 1167
BstX2I RGATCY 3 cut(s) 130, 360, 754
BstYI RGATCY 3 cut(s) 130, 360, 754
Bsu36I CCTNAGG 1 cut(s) 606
BsuI GTATCC 1 cut(s) 448
BsuRI GGCC 1 cut(s) 1202
BtgI CCRYGG 1 cut(s) 72
BtgZI GCGATG 2 cut(s) 97, 1021
BtsCI GGATG 2 cut(s) 514, 793
BtsIMutI CAGTG 1 cut(s) 173
BveI ACCTGC 1 cut(s) 178
Cac8I GCNNGC 3 cut(s) 104, 897, 1094
CaiI CAGNNNCTG 3 cut(s) 88, 417, 690
Cfr13I GGNCC 2 cut(s) 641, 1568
Csp6I GTAC 4 cut(s) 22, 421, 709, 735
CviAII CATG 5 cut(s) 73, 1020, 1155, 1186, 1319
CviQI GTAC 4 cut(s) 22, 421, 709, 735
DdeI CTNAG 8 cut(s) 89, 219, 235, 282, 606, 980, 1104, 1545
DpnI GATC 4 cut(s) 17, 132, 362, 756
DpnII GATC 4 cut(s) 15, 130, 360, 754
DriI GACNNNNNGTC 1 cut(s) 639
Eam1104I CTCTTC 2 cut(s) 579, 819
Eam1105I GACNNNNNGTC 1 cut(s) 639
EarI CTCTTC 2 cut(s) 579, 819
Ecl136II GAGCTC 2 cut(s) 93, 602
Eco130I CCWWGG 2 cut(s) 72, 1479
Eco147I AGGCCT 1 cut(s) 1202
Eco24I GRGCYC 3 cut(s) 95, 402, 604
Eco47I GGWCC 2 cut(s) 641, 1568
Eco53kI GAGCTC 2 cut(s) 93, 602
Eco57I CTGAAG 3 cut(s) 111, 197, 603
Eco81I CCTNAGG 1 cut(s) 606
EcoICRI GAGCTC 2 cut(s) 93, 602
EcoO109I RGGNCCY 2 cut(s) 641, 1568
EcoRI GAATTC 1 cut(s) 1232
EcoRII CCWGG 2 cut(s) 637, 943
EcoT14I CCWWGG 2 cut(s) 72, 1479
EcoT22I ATGCAT 2 cut(s) 436, 908
EcoT38I GRGCYC 3 cut(s) 95, 402, 604
ErhI CCWWGG 2 cut(s) 72, 1479
FaeI CATG 5 cut(s) 76, 1023, 1158, 1189, 1322
FalI AAGNNNNNCTT 2 cut(s) 1377, 1409
FaqI GGGAC 1 cut(s) 194
FatI CATG 5 cut(s) 72, 1019, 1154, 1185, 1318
FauI CCCGC 3 cut(s) 111, 1510, 1579
FauNDI CATATG 2 cut(s) 436, 1008
Fnu4HI GCNGC 5 cut(s) 319, 620, 653, 1515, 1583
FokI GGATG 2 cut(s) 521, 800
FriOI GRGCYC 3 cut(s) 95, 402, 604
Fsp4HI GCNGC 5 cut(s) 319, 620, 653, 1515, 1583
FspBI CTAG 3 cut(s) 954, 1194, 1380
GluI GCNGC 5 cut(s) 319, 620, 653, 1515, 1583
GsaI CCCAGC 1 cut(s) 153
GsuI CTGGAG 1 cut(s) 621
HaeIII GGCC 1 cut(s) 1202
Hin1II CATG 5 cut(s) 76, 1023, 1158, 1189, 1322
HindIII AAGCTT 1 cut(s) 270
HphI GGTGA 2 cut(s) 159, 901
Hpy166II GTNNAC 2 cut(s) 497, 709
Hpy188III TCNNGA 7 cut(s) 194, 247, 327, 752, 1069, 1409, 1465
Hpy8I GTNNAC 2 cut(s) 497, 709
HpyAV CCTTC 6 cut(s) 47, 135, 666, 778, 884, 1213
HpyCH4III ACNGT 3 cut(s) 205, 666, 885
HpyCH4V TGCA 7 cut(s) 173, 434, 899, 906, 1092, 1158, 1490
HpyF10VI GCNNNNNNNGC 2 cut(s) 99, 658
HpyF3I CTNAG 8 cut(s) 89, 219, 235, 282, 606, 980, 1104, 1545
Hsp92II CATG 5 cut(s) 76, 1023, 1158, 1189, 1322
Kzo9I GATC 4 cut(s) 15, 130, 360, 754
LmnI GCTCC 2 cut(s) 607, 1459
Lsp1109I GCAGC 3 cut(s) 330, 631, 1594
LweI GCATC 3 cut(s) 18, 915, 1477
MaeI CTAG 3 cut(s) 954, 1194, 1380
MalI GATC 4 cut(s) 17, 132, 362, 756
MbiI CCGCTC 1 cut(s) 106
MboI GATC 4 cut(s) 15, 130, 360, 754
MboII GAAGA 5 cut(s) 77, 239, 596, 836, 1167
MfeI CAATTG 1 cut(s) 459
MflI RGATCY 3 cut(s) 130, 360, 754
MhlI GDGCHC 4 cut(s) 95, 339, 402, 604
MluCI AATT 6 cut(s) 184, 459, 475, 1125, 1232, 1281
MlyI GAGTC 3 cut(s) 628, 722, 1183
MmeI TCCRAC 2 cut(s) 451, 867
Mph1103I ATGCAT 2 cut(s) 436, 908
MroXI GAANNNNTTC 2 cut(s) 577, 1270
MseI TTAA 4 cut(s) 353, 1128, 1259, 1280
MspR9I CCNGG 2 cut(s) 639, 945
MunI CAATTG 1 cut(s) 459
MvaI CCWGG 2 cut(s) 639, 945
MwoI GCNNNNNNNGC 2 cut(s) 99, 658
NcoI CCATGG 1 cut(s) 72
NdeI CATATG 2 cut(s) 436, 1008
NdeII GATC 4 cut(s) 15, 130, 360, 754
NlaIII CATG 5 cut(s) 76, 1023, 1158, 1189, 1322
NlaIV GGNNCC 3 cut(s) 146, 1120, 1570
NsiI ATGCAT 2 cut(s) 436, 908
NspV TTCGAA 1 cut(s) 40
PaqCI CACCTGC 1 cut(s) 178
PceI AGGCCT 1 cut(s) 1202
PcsI WCGNNNNNNNCGW 1 cut(s) 201
PdmI GAANNNNTTC 2 cut(s) 577, 1270
PfeI GAWTC 9 cut(s) 10, 68, 77, 85, 164, 377, 440, 578, 1271
PflMI CCANNNNNTGG 2 cut(s) 1292, 1319
PkrI GCNGC 5 cut(s) 320, 621, 654, 1516, 1584
PleI GAGTC 3 cut(s) 628, 721, 1183
PpsI GAGTC 3 cut(s) 628, 721, 1183
PpuMI RGGWCCY 2 cut(s) 641, 1568
PsiI TTATAA 2 cut(s) 843, 1373
Psp124BI GAGCTC 2 cut(s) 95, 604
Psp5II RGGWCCY 2 cut(s) 641, 1568
Psp6I CCWGG 2 cut(s) 637, 943
PspFI CCCAGC 1 cut(s) 149
PspGI CCWGG 2 cut(s) 637, 943
PspN4I GGNNCC 3 cut(s) 146, 1120, 1570
PspPI GGNCC 2 cut(s) 641, 1568
PspPPI RGGWCCY 2 cut(s) 641, 1568
PstNI CAGNNNCTG 3 cut(s) 88, 417, 690
PsuI RGATCY 3 cut(s) 130, 360, 754
RsaI GTAC 4 cut(s) 23, 422, 710, 736
RsaNI GTAC 4 cut(s) 22, 421, 709, 735
SacI GAGCTC 2 cut(s) 95, 604
SaqAI TTAA 4 cut(s) 353, 1128, 1259, 1280
SatI GCNGC 5 cut(s) 319, 620, 653, 1515, 1583
Sau3AI GATC 4 cut(s) 15, 130, 360, 754
Sau96I GGNCC 2 cut(s) 641, 1568
ScaI AGTACT 1 cut(s) 23
SchI GAGTC 3 cut(s) 628, 722, 1183
ScrFI CCNGG 2 cut(s) 639, 945
SduI GDGCHC 4 cut(s) 95, 339, 402, 604
SfaNI GCATC 3 cut(s) 18, 915, 1477
SfuI TTCGAA 1 cut(s) 40
SinI GGWCC 2 cut(s) 641, 1568
SmlI CTYRAG 4 cut(s) 424, 697, 1001, 1592
SmoI CTYRAG 4 cut(s) 424, 697, 1001, 1592
Sse9I AATT 6 cut(s) 184, 459, 475, 1125, 1232, 1281
SseBI AGGCCT 1 cut(s) 1202
SsiI CCGC 7 cut(s) 104, 653, 869, 1430, 1514, 1517, 1572
SspI AATATT 1 cut(s) 1498
SspMI CTAG 3 cut(s) 954, 1194, 1380
SstI GAGCTC 2 cut(s) 95, 604
StuI AGGCCT 1 cut(s) 1202
StyD4I CCNGG 2 cut(s) 637, 943
StyI CCWWGG 2 cut(s) 72, 1479
TaaI ACNGT 3 cut(s) 205, 666, 885
TaqI TCGA 8 cut(s) 26, 40, 195, 246, 751, 1056, 1564, 1588
TasI AATT 6 cut(s) 184, 459, 475, 1125, 1232, 1281
TatI WGTACW 2 cut(s) 21, 734
TauI GCSGC 2 cut(s) 655, 1517
TfiI GAWTC 9 cut(s) 10, 68, 77, 85, 164, 377, 440, 578, 1271
Tru1I TTAA 4 cut(s) 353, 1128, 1259, 1280
Tru9I TTAA 4 cut(s) 353, 1128, 1259, 1280
TscAI CASTG 1 cut(s) 180
TseI GCWGC 3 cut(s) 318, 619, 1582
TspDTI ATGAA 7 cut(s) 177, 282, 453, 837, 861, 1365, 1490
TspGWI ACGGA 2 cut(s) 50, 708
TspRI CASTG 1 cut(s) 180
Van91I CCANNNNNTGG 2 cut(s) 1292, 1319
VpaK11BI GGWCC 2 cut(s) 641, 1568
XapI RAATTY 2 cut(s) 475, 1232
XmnI GAANNNNTTC 2 cut(s) 577, 1270
XspI CTAG 3 cut(s) 954, 1194, 1380
ZrmI AGTACT 1 cut(s) 23
Zsp2I ATGCAT 2 cut(s) 436, 908
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.