pycom15g22720

Regulator of chromosome condensation (RCC1) repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0000878
Physical Location & Seq
Reverse (-)
16800484 .. 16802592
2109 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g22720.2

Sequence Viewer

Length: 1338 bp
ATGAAAGGAGGACTGGGATCCCTGGTGAAGGGGCCTTGTGCGGTTAACGCGATCATTCAAAAGCTGCAGAGATGTATGAGCAGCGGAACCGCCGCAGTCATGAGCTTTGGCGACGGAAACCACGGTGCTCTGGGCCTGCCGACTTCCCTCATGGGACTCGGCGTTGACGCTTACGAGCCCATCCGGGTCCCTTCTCTGCCTCCTGATGTCACCAGCGTCGCCGCCGGGCACTACCACTCCCTCGCCGTCACTTCCCAAGGGCAGCTCTGGGCTTGGGGCCGAGACCTTGAAGCCCAACTCGGCCGCGGACTGCCTTCTCCCAGAGATTCATGGAATGAACCAAAGAGAGTAACTGGGTTGGATCAAGTAAACGTTTGTGCTGCATTTGCGTCTGGTGTTGTTTCTGCAGCCATTGGAGATGATGGTTCTTTGTGGGTTTGGGGAAAGTCGAAGCGCGGCCAGCTTGGTCTTGGAAATCAAGTCACTGAGGCTGTGGTACCTTCCAGAGTTGAAGCACTATCGGGAGAGAAAATAATTAAGGTATCATTCGGTTGGGGGCATGCTCTTGCACTGACTGAGGATGGAAAATTGTTTGGTTGGGGTTATTCAGCCGATGGTAGGTTAGGAAGCATTACAGAATCCTTGGAGACGTCTCTACTGGAATCACGGGCAGGTTTGAGTGATAAGGAACTATCAAGTTCAACTGTGGAAGCTGCGGAGAAGCTGGTTTTAGAAGGAATGGCTAAGGAGAATGACATGCCGATAGTTTGGGAACCTAGTTTAGTGAGAGAACTACATTCTGTTGAAGTTGTAGACATTTCATGTGGTTTTGATCATTCATTAATTCTTTGCGGTGATGGAACACTACTAAGTTGTGGGAGCAACGTATATGGTCAGTTGGGCAGAGAAAAACAGGATTTGGGATTGTTCCCTGTTGACATAAGCTTCCGTCCCACATCTATAGCATCAGGACTTGGCCATTCTTTGGCAATTTGCCAGGTTCCATCGTCGAATGTTATAAAAGGAGCCGAAGACATTGTTACATGGGGGTGGAATCAAAGTTCTCAGCTTGGAAGAGGTGGATCGGAGAATATTCCATTGGTGGTTGAAGGATTGGAAGGGGAAATTCCTGTCTCAGTGTCAGGAGGGCGTGTGCATTCCATTGCTCTCACATCCAAGGGAGAAGTGTGGGTTTGGGGTTGTGGTAAGAATGGAAGATTAGGGTTAGGAAGCTCCTGTGATGAAGCAGAACCGATTTTGCTCGACTCTGTAGAGGGTTGTCAAGTTTTACAAGCTGTCTCTGGATTTGATCATAATCTTGTCCTGATTGCGGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RCC1 RCC1_2

Protein Analysis

446

Amino Acids

46.55

Weight (kDa)

4.95

Isoelectric Point (pI)

34.06

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1019
AatII GACGTC 1 cut(s) 653
Acc36I ACCTGC 1 cut(s) 662
Acc65I GGTACC 1 cut(s) 496
AccB1I GGYRCC 1 cut(s) 496
AccB7I CCANNNNNTGG 1 cut(s) 985
AccI GTMKAC 1 cut(s) 813
AccII CGCG 3 cut(s) 50, 306, 456
AclI AACGTT 1 cut(s) 372
AclWI GGATC 4 cut(s) 12, 25, 369, 1090
AcoI YGGCCR 3 cut(s) 301, 457, 976
AcsI RAATTY 1 cut(s) 1125
AcyI GRCGYC 1 cut(s) 650
AfaI GTAC 1 cut(s) 498
AfiI CCNNNNNNNGG 4 cut(s) 28, 618, 985, 1330
AgsI TTSAA 6 cut(s) 59, 290, 512, 702, 806, 1109
AjnI CCWGG 2 cut(s) 21, 996
AjuI GAANNNNNNNTTGG 2 cut(s) 1082, 1114
AloI GAACNNNNNNTCC 2 cut(s) 1045, 1077
Alw21I GWGCWC 1 cut(s) 130
Alw26I GTCTC 5 cut(s) 276, 641, 657, 1138, 1303
AlwI GGATC 4 cut(s) 12, 25, 369, 1090
AoxI GGCC 6 cut(s) 32, 133, 277, 301, 457, 976
ApeKI GCWGC 6 cut(s) 64, 81, 262, 380, 407, 713
ApoI RAATTY 1 cut(s) 1125
AseI ATTAAT 1 cut(s) 842
Asp718I GGTACC 1 cut(s) 496
AspLEI GCGC 1 cut(s) 456
AspS9I GGNCC 4 cut(s) 32, 133, 187, 277
AsuC2I CCSGG 2 cut(s) 185, 226
AsuHPI GGTGA 3 cut(s) 37, 202, 866
AvaII GGWCC 1 cut(s) 187
BaeGI GKGCMC 1 cut(s) 231
BalI TGGCCA 1 cut(s) 978
BamHI GGATCC 1 cut(s) 17
BanI GGYRCC 1 cut(s) 496
BanII GRGCYC 1 cut(s) 180
BbsI GAAGAC 1 cut(s) 1038
Bbv12I GWGCWC 1 cut(s) 130
BbvI GCAGC 6 cut(s) 51, 93, 274, 367, 419, 700
BccI CCATC 6 cut(s) 188, 416, 575, 608, 851, 1012
BceAI ACGGC 1 cut(s) 230
BciT130I CCWGG 2 cut(s) 23, 998
BclI TGATCA 2 cut(s) 832, 1309
BcnI CCSGG 2 cut(s) 185, 226
BcoDI GTCTC 5 cut(s) 276, 641, 657, 1138, 1303
BfaI CTAG 1 cut(s) 777
BfmI CTRYAG 4 cut(s) 65, 405, 960, 1269
BfuAI ACCTGC 1 cut(s) 662
Bme1390I CCNGG 4 cut(s) 23, 185, 226, 998
Bme18I GGWCC 1 cut(s) 187
BmgT120I GGNCC 4 cut(s) 32, 133, 187, 277
BmrFI CCNGG 4 cut(s) 23, 185, 226, 998
BmrI ACTGGG 2 cut(s) 23, 363
BmsI GCATC 1 cut(s) 974
BmuI ACTGGG 2 cut(s) 23, 363
BpiI GAAGAC 1 cut(s) 1038
Bpu10I CCTNAGC 1 cut(s) 744
BpuMI CCSGG 2 cut(s) 185, 226
BsaBI GATNNNNATC 1 cut(s) 1314
BsaHI GRCGYC 1 cut(s) 650
BsaI GGTCTC 1 cut(s) 276
BsaJI CCNNGG 6 cut(s) 21, 121, 256, 304, 642, 1176
BsaXI ACNNNNNCTCC 4 cut(s) 221, 251, 1137, 1167
Bsc4I CCNNNNNNNGG 4 cut(s) 28, 618, 985, 1330
Bse1I ACTGG 3 cut(s) 18, 358, 663
Bse3DI GCAATG 1 cut(s) 1161
Bse8I GATNNNNATC 1 cut(s) 1314
BseBI CCWGG 2 cut(s) 23, 998
BseDI CCNNGG 6 cut(s) 21, 121, 256, 304, 642, 1176
BseGI GGATG 3 cut(s) 180, 586, 1172
BseJI GATNNNNATC 1 cut(s) 1314
BseLI CCNNNNNNNGG 4 cut(s) 28, 618, 985, 1330
BseMI GCAATG 1 cut(s) 1161
BseMII CTCAG 4 cut(s) 477, 567, 1079, 1149
BseNI ACTGG 3 cut(s) 18, 358, 663
BseSI GKGCMC 1 cut(s) 231
BseX3I CGGCCG 1 cut(s) 301
BseXI GCAGC 6 cut(s) 51, 93, 274, 367, 419, 700
Bsh1236I CGCG 3 cut(s) 50, 306, 456
Bsh1285I CGRYCG 1 cut(s) 304
BshFI GGCC 6 cut(s) 34, 135, 279, 303, 459, 978
BshNI GGYRCC 1 cut(s) 496
BsiEI CGRYCG 1 cut(s) 304
BsiHKAI GWGCWC 1 cut(s) 130
BsiSI CCGG 2 cut(s) 184, 225
BslFI GGGAC 3 cut(s) 168, 173, 936
BslI CCNNNNNNNGG 4 cut(s) 28, 618, 985, 1330
BsmAI GTCTC 5 cut(s) 276, 641, 657, 1138, 1303
BsmBI CGTCTC 2 cut(s) 641, 657
BsmFI GGGAC 3 cut(s) 168, 173, 936
BsmI GAATGC 1 cut(s) 1156
BsnI GGCC 6 cut(s) 34, 135, 279, 303, 459, 978
Bso31I GGTCTC 1 cut(s) 276
Bsp1286I GDGCHC 3 cut(s) 130, 180, 231
Bsp143I GATC 6 cut(s) 17, 51, 361, 832, 1082, 1309
BspANI GGCC 6 cut(s) 34, 135, 279, 303, 459, 978
BspCNI CTCAG 4 cut(s) 478, 568, 1078, 1148
BspFNI CGCG 3 cut(s) 50, 306, 456
BspHI TCATGA 1 cut(s) 99
BspMAI CTGCAG 2 cut(s) 69, 409
BspMI ACCTGC 1 cut(s) 662
BspPI GGATC 4 cut(s) 12, 25, 369, 1090
BspT107I GGYRCC 1 cut(s) 496
BspTNI GGTCTC 1 cut(s) 276
BsrDI GCAATG 1 cut(s) 1161
BsrI ACTGG 3 cut(s) 18, 358, 663
BssECI CCNNGG 6 cut(s) 21, 121, 256, 304, 642, 1176
BssMI GATC 6 cut(s) 17, 51, 361, 832, 1082, 1309
BssNI GRCGYC 1 cut(s) 650
BssT1I CCWWGG 3 cut(s) 256, 642, 1176
Bst2UI CCWGG 2 cut(s) 23, 998
Bst4CI ACNGT 2 cut(s) 125, 706
Bst6I CTCTTC 1 cut(s) 1069
BstACI GRCGYC 1 cut(s) 650
BstC8I GCNNGC 3 cut(s) 137, 461, 561
BstDEI CTNAG 6 cut(s) 486, 576, 744, 869, 1065, 1135
BstDSI CCRYGG 2 cut(s) 121, 304
BstENI CCTNNNNNAGG 1 cut(s) 26
BstF5I GGATG 3 cut(s) 180, 586, 1172
BstFNI CGCG 3 cut(s) 50, 306, 456
BstHHI GCGC 1 cut(s) 456
BstKTI GATC 6 cut(s) 20, 54, 364, 835, 1085, 1312
BstMAI GTCTC 5 cut(s) 276, 641, 657, 1138, 1303
BstMBI GATC 6 cut(s) 17, 51, 361, 832, 1082, 1309
BstMCI CGRYCG 1 cut(s) 304
BstMWI GCNNNNNNNGC 3 cut(s) 47, 386, 460
BstNI CCWGG 2 cut(s) 23, 998
BstNSI RCATGY 2 cut(s) 563, 760
BstSCI CCNGG 4 cut(s) 21, 183, 224, 996
BstSFI CTRYAG 4 cut(s) 65, 405, 960, 1269
BstSLI GKGCMC 1 cut(s) 231
BstUI CGCG 3 cut(s) 50, 306, 456
BstV1I GCAGC 6 cut(s) 51, 93, 274, 367, 419, 700
BstV2I GAAGAC 1 cut(s) 1038
BstX2I RGATCY 1 cut(s) 17
BstYI RGATCY 1 cut(s) 17
BstZI CGGCCG 1 cut(s) 301
BsuRI GGCC 6 cut(s) 34, 135, 279, 303, 459, 978
BtgI CCRYGG 2 cut(s) 121, 304
BtsCI GGATG 3 cut(s) 180, 586, 1172
BtsIMutI CAGTG 3 cut(s) 483, 569, 1143
BveI ACCTGC 1 cut(s) 662
Cac8I GCNNGC 3 cut(s) 137, 461, 561
CciI TCATGA 1 cut(s) 99
CfoI GCGC 1 cut(s) 456
Cfr13I GGNCC 4 cut(s) 32, 133, 187, 277
Cfr42I CCGCGG 1 cut(s) 307
CseI GACGC 3 cut(s) 176, 205, 378
Csp6I GTAC 1 cut(s) 497
CviAII CATG 7 cut(s) 100, 151, 330, 560, 757, 822, 1044
CviQI GTAC 1 cut(s) 497
DdeI CTNAG 6 cut(s) 486, 576, 744, 869, 1065, 1135
DpnI GATC 6 cut(s) 19, 53, 363, 834, 1084, 1311
DpnII GATC 6 cut(s) 17, 51, 361, 832, 1082, 1309
EaeI YGGCCR 3 cut(s) 301, 457, 976
EagI CGGCCG 1 cut(s) 301
Eam1104I CTCTTC 1 cut(s) 1069
EarI CTCTTC 1 cut(s) 1069
EclXI CGGCCG 1 cut(s) 301
Eco130I CCWWGG 3 cut(s) 256, 642, 1176
Eco24I GRGCYC 1 cut(s) 180
Eco31I GGTCTC 1 cut(s) 276
Eco47I GGWCC 1 cut(s) 187
Eco52I CGGCCG 1 cut(s) 301
EcoNI CCTNNNNNAGG 1 cut(s) 26
EcoO109I RGGNCCY 2 cut(s) 32, 187
EcoRII CCWGG 2 cut(s) 21, 996
EcoT14I CCWWGG 3 cut(s) 256, 642, 1176
EcoT38I GRGCYC 1 cut(s) 180
ErhI CCWWGG 3 cut(s) 256, 642, 1176
Esp3I CGTCTC 2 cut(s) 641, 657
FaeI CATG 7 cut(s) 103, 154, 333, 563, 760, 825, 1047
FaqI GGGAC 3 cut(s) 168, 173, 936
FatI CATG 7 cut(s) 99, 150, 329, 559, 756, 821, 1043
FbaI TGATCA 2 cut(s) 832, 1309
FblI GTMKAC 1 cut(s) 813
FokI GGATG 3 cut(s) 167, 593, 1159
FriOI GRGCYC 1 cut(s) 180
FspBI CTAG 1 cut(s) 777
GlaI GCGC 1 cut(s) 455
HaeIII GGCC 6 cut(s) 34, 135, 279, 303, 459, 978
HapII CCGG 2 cut(s) 184, 225
HgaI GACGC 3 cut(s) 176, 205, 378
HhaI GCGC 1 cut(s) 456
Hin1I GRCGYC 1 cut(s) 650
Hin1II CATG 7 cut(s) 103, 154, 333, 563, 760, 825, 1047
Hin6I GCGC 1 cut(s) 454
HinP1I GCGC 1 cut(s) 454
HincII GTYRAC 3 cut(s) 46, 166, 937
HindII GTYRAC 3 cut(s) 46, 166, 937
HindIII AAGCTT 1 cut(s) 943
HinfI GANTC 6 cut(s) 156, 326, 638, 662, 1054, 1265
HpaI GTTAAC 1 cut(s) 46
HpaII CCGG 2 cut(s) 184, 225
HphI GGTGA 3 cut(s) 37, 202, 866
Hpy166II GTNNAC 5 cut(s) 46, 166, 370, 814, 937
Hpy188I TCNGA 1 cut(s) 1087
Hpy188III TCNNGA 8 cut(s) 100, 203, 504, 522, 969, 1143, 1302, 1324
Hpy8I GTNNAC 5 cut(s) 46, 166, 370, 814, 937
Hpy99I CGWCG 3 cut(s) 116, 221, 1012
HpyAV CCTTC 7 cut(s) 22, 201, 324, 510, 728, 1103, 1112
HpyCH4III ACNGT 2 cut(s) 125, 706
HpyCH4IV ACGT 3 cut(s) 372, 650, 885
HpyCH4V TGCA 5 cut(s) 67, 383, 407, 569, 1156
HpyF10VI GCNNNNNNNGC 3 cut(s) 47, 386, 460
HpyF3I CTNAG 6 cut(s) 486, 576, 744, 869, 1065, 1135
HpySE526I ACGT 3 cut(s) 372, 650, 885
Hsp92I GRCGYC 1 cut(s) 650
Hsp92II CATG 7 cut(s) 103, 154, 333, 563, 760, 825, 1047
HspAI GCGC 1 cut(s) 454
KflI GGGWCCC 1 cut(s) 187
KpnI GGTACC 1 cut(s) 500
Ksp22I TGATCA 2 cut(s) 832, 1309
KspAI GTTAAC 1 cut(s) 46
KspI CCGCGG 1 cut(s) 307
Kzo9I GATC 6 cut(s) 17, 51, 361, 832, 1082, 1309
LmnI GCTCC 3 cut(s) 879, 1025, 1238
Lsp1109I GCAGC 6 cut(s) 51, 93, 274, 367, 419, 700
LweI GCATC 1 cut(s) 974
MaeI CTAG 1 cut(s) 777
MaeII ACGT 3 cut(s) 372, 650, 885
MaeIII GTNAC 5 cut(s) 208, 247, 349, 481, 1039
MalI GATC 6 cut(s) 19, 53, 363, 834, 1084, 1311
MboI GATC 6 cut(s) 17, 51, 361, 832, 1082, 1309
MboII GAAGA 3 cut(s) 1043, 1086, 1227
MflI RGATCY 1 cut(s) 17
MhlI GDGCHC 3 cut(s) 130, 180, 231
MlsI TGGCCA 1 cut(s) 978
MluCI AATT 5 cut(s) 534, 587, 843, 990, 1125
MluNI TGGCCA 1 cut(s) 978
MlyI GAGTC 2 cut(s) 150, 1259
MmeI TCCRAC 1 cut(s) 339
MnlI CCTC 8 cut(s) 158, 210, 251, 481, 571, 1070, 1139, 1267
Mox20I TGGCCA 1 cut(s) 978
MscI TGGCCA 1 cut(s) 978
MseI TTAA 3 cut(s) 45, 537, 842
MslI CAYNNNNRTG 1 cut(s) 1048
Msp20I TGGCCA 1 cut(s) 978
MspA1I CMGCKG 2 cut(s) 84, 306
MspI CCGG 2 cut(s) 184, 225
MspR9I CCNGG 4 cut(s) 23, 185, 226, 998
Mva1269I GAATGC 1 cut(s) 1156
MvaI CCWGG 2 cut(s) 23, 998
MvnI CGCG 3 cut(s) 50, 306, 456
MwoI GCNNNNNNNGC 3 cut(s) 47, 386, 460
NciI CCSGG 2 cut(s) 185, 226
NdeII GATC 6 cut(s) 17, 51, 361, 832, 1082, 1309
NlaIII CATG 7 cut(s) 103, 154, 333, 563, 760, 825, 1047
NmeAIII GCCGAG 3 cut(s) 138, 279, 305
NmuCI GTSAC 3 cut(s) 208, 247, 481
NspI RCATGY 2 cut(s) 563, 760
PaeI GCATGC 1 cut(s) 563
PagI TCATGA 1 cut(s) 99
PctI GAATGC 1 cut(s) 1156
PfeI GAWTC 4 cut(s) 326, 638, 662, 1054
PflMI CCANNNNNTGG 1 cut(s) 985
PleI GAGTC 2 cut(s) 150, 1259
PpsI GAGTC 2 cut(s) 150, 1259
PpuMI RGGWCCY 1 cut(s) 187
PshBI ATTAAT 1 cut(s) 842
PsiI TTATAA 1 cut(s) 1019
Psp1406I AACGTT 1 cut(s) 372
Psp5II RGGWCCY 1 cut(s) 187
Psp6I CCWGG 2 cut(s) 21, 996
PspGI CCWGG 2 cut(s) 21, 996
PspPI GGNCC 4 cut(s) 32, 133, 187, 277
PspPPI RGGWCCY 1 cut(s) 187
PstI CTGCAG 2 cut(s) 69, 409
PsuI RGATCY 1 cut(s) 17
RsaI GTAC 1 cut(s) 498
RsaNI GTAC 1 cut(s) 497
RseI CAYNNNNRTG 1 cut(s) 1048
SacII CCGCGG 1 cut(s) 307
SaqAI TTAA 3 cut(s) 45, 537, 842
Sau3AI GATC 6 cut(s) 17, 51, 361, 832, 1082, 1309
Sau96I GGNCC 4 cut(s) 32, 133, 187, 277
SchI GAGTC 2 cut(s) 150, 1259
ScrFI CCNGG 4 cut(s) 23, 185, 226, 998
SduI GDGCHC 3 cut(s) 130, 180, 231
SfaNI GCATC 1 cut(s) 974
SfcI CTRYAG 4 cut(s) 65, 405, 960, 1269
Sfr303I CCGCGG 1 cut(s) 307
SgrBI CCGCGG 1 cut(s) 307
SinI GGWCC 1 cut(s) 187
SmiMI CAYNNNNRTG 1 cut(s) 1048
SphI GCATGC 1 cut(s) 563
Sse9I AATT 5 cut(s) 534, 587, 843, 990, 1125
SspI AATATT 1 cut(s) 1093
SspMI CTAG 1 cut(s) 777
StyD4I CCNGG 4 cut(s) 21, 183, 224, 996
StyI CCWWGG 3 cut(s) 256, 642, 1176
TaaI ACNGT 2 cut(s) 125, 706
TaiI ACGT 3 cut(s) 375, 653, 888
TaqI TCGA 3 cut(s) 449, 1010, 1263
TasI AATT 5 cut(s) 534, 587, 843, 990, 1125
TauI GCSGC 4 cut(s) 95, 224, 306, 459
TfiI GAWTC 4 cut(s) 326, 638, 662, 1054
Tru1I TTAA 3 cut(s) 45, 537, 842
Tru9I TTAA 3 cut(s) 45, 537, 842
TscAI CASTG 3 cut(s) 490, 576, 1143
TseFI GTSAC 3 cut(s) 208, 247, 481
TseI GCWGC 6 cut(s) 64, 81, 262, 380, 407, 713
Tsp45I GTSAC 3 cut(s) 208, 247, 481
TspDTI ATGAA 6 cut(s) 17, 318, 351, 810, 828, 1257
TspGWI ACGGA 2 cut(s) 129, 938
TspRI CASTG 3 cut(s) 490, 576, 1143
Van91I CCANNNNNTGG 1 cut(s) 985
VpaK11BI GGWCC 1 cut(s) 187
VspI ATTAAT 1 cut(s) 842
XagI CCTNNNNNAGG 1 cut(s) 26
XapI RAATTY 1 cut(s) 1125
XceI RCATGY 2 cut(s) 563, 760
XcmI CCANNNNNNNNNTGG 1 cut(s) 467
XmiI GTMKAC 1 cut(s) 813
XspI CTAG 1 cut(s) 777
ZraI GACGTC 1 cut(s) 651
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.