Rw2G011320

Regulator of chromosome condensation (RCC1) repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000878
Physical Location & Seq
Forward (+)
11433041 .. 11435029
1989 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G011320.1

Sequence Viewer

Length: 1314 bp
ATGCCAGGACTGGGATCCCTGGTGAAGAACCAGCGTCTAGTCCAAAGGTTTCAGAGATGCATGACCACGACAGCAGCCGTCATGAGCTTCGGGGACGGCAACCATGGCGCTCTGGGCTTGCCCACTTCCTTAATGGGCCTTGGTGTGGACGCCTACGAGCCCACCCGGGTCCCTTCTCTGCCTCCTGACATCACCAGCGTCACCGCCGGGCATTACCACTCCCTGGCCGTCACTTCCGAAGGCCAGCTCTGGGCTTGGGGCCGAGACCTCGAGGCCCAGCTCGGCCGTGGAGGTCCTTCTCCCAGAGATTTGTGGAATGAACCAAGGAGAGTAACAGGATTGGATCAAGTAAATGTTTGTTCTGCATTTGCATCCGGTGTCATTTCTGCAGCCATTGGAGATGATGGTTCTTTGTGGGTTTGGGGAAAGTCAAAGCGTGGCCAGCTTGGTCTGGGAGAGCAGGTCATGGAGGCTGTGGTTCCTTCCCGAGTTGAAGCACTTGCTGGAGAAAAAATAACTAAGGTTGCACTTGGTTGGGGGCATGCTCTTGCATTGACTGAGGATGGAAAGTTATTTGGTTGGGGTTATTTTGCTGATGGTAGGTTAGGAAGCATTAAAGAATCTTTGGAGACATCTCAACTGGAATATACTTCCAATAATCAAGAACTGTCGAGTTCATCTTTGGAAGCTGCCGAGAAGCTGGTTTTAGAAGGAATGGCCAAGGAGAATAACATGCCAATAGTTTGGGAACCCAGTTTAGTGAGGGAACTACAGTGTATTGAAGTTGTAGACATTGCATGTGGTCTTGATCATTCATTAATTCTTTGTGGTGATGGGACGCTACTGAGCTGTGGGAGCAATGTATATGGTCAGTTGGGAAGAGAAAAACAGGATGTAGGATTGTTTCCTGTTGATATAAGCTTCCGTCCAATGTCTATAGCAGCCGGGCTTGGCCACTCTTTGGCAATTTGCCAGGTTCCATCATCAGATGTTATAGGAGGAGCCGCAGGTGTCATTACATGGGGTTGGAATCAGACCTCTCAGCTCGGTAGGGATGGGCCAGAAAATATTCCATTAGGAGTTGAAGGCTTGGAAGGGGAAACTCCTGTATCAGTAGCAGGAGGTCGTGTGCATTCCATTGCTCTAACATCAGAGAGAGAAGTGTGGGTTTGGGGTAGTGGTAAGAATGGAAGGCTTGGGTTAGGAAGCTCCTGTGATGAACCTGAACCAATTTTGCTGGACTCTGTAGAGGGTTGTGATGTTTTACAAGCTGTGTCAGGGTTTGATCATAATCTTCTCTTAATTGCCGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RCC1 RCC1_2

Protein Analysis

437

Amino Acids

46.2

Weight (kDa)

4.75

Isoelectric Point (pI)

37.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_RLD PF25390 27 - 432 3e-81 RCC1-like domain
RCC1 PF00415 29 - 77 3.3e-07 Regulator of chromosome condensation (RCC1) repeat
RCC1_2 PF13540 64 - 89 1.2e-07 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 80 - 132 3.8e-06 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 135 - 185 1.4e-12 Regulator of chromosome condensation (RCC1) repeat
RCC1_2 PF13540 172 - 199 1.3e-08 Regulator of chromosome condensation (RCC1) repeat
RCC1_2 PF13540 262 - 291 1e-09 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 278 - 323 6e-07 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 338 - 382 1.7e-07 Regulator of chromosome condensation (RCC1) repeat
RCC1_2 PF13540 370 - 397 1e-05 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 387 - 434 3e-08 Regulator of chromosome condensation (RCC1) repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 998
AbsI CCTCGAGG 1 cut(s) 269
Acc36I ACCTGC 2 cut(s) 451, 998
AccB7I CCANNNNNTGG 2 cut(s) 223, 961
AccI GTMKAC 1 cut(s) 789
AciI CCGC 2 cut(s) 204, 1005
AclWI GGATC 3 cut(s) 9, 22, 351
AcoI YGGCCR 5 cut(s) 225, 283, 439, 717, 952
AcyI GRCGYC 1 cut(s) 150
AfiI CCNNNNNNNGG 5 cut(s) 11, 145, 223, 250, 961
AgsI TTSAA 3 cut(s) 494, 782, 1085
AjnI CCWGG 4 cut(s) 4, 18, 222, 972
Alw26I GTCTC 2 cut(s) 258, 623
AlwI GGATC 3 cut(s) 9, 22, 351
Ama87I CYCGRG 3 cut(s) 165, 269, 486
ApeKI GCWGC 4 cut(s) 74, 389, 689, 941
AseI ATTAAT 1 cut(s) 818
Asp700I GAANNNNTTC 1 cut(s) 1068
AspLEI GCGC 1 cut(s) 110
AspS9I GGNCC 6 cut(s) 136, 169, 259, 274, 293, 1058
AsuC2I CCSGG 4 cut(s) 166, 167, 208, 946
AsuHPI GGTGA 4 cut(s) 34, 184, 193, 842
AvaI CYCGRG 3 cut(s) 165, 269, 486
AvaII GGWCC 2 cut(s) 169, 293
BaeI ACNNNNGTAYC 2 cut(s) 1092, 1125
BalI TGGCCA 3 cut(s) 441, 719, 954
BamHI GGATCC 1 cut(s) 14
BanII GRGCYC 1 cut(s) 162
BbvI GCAGC 4 cut(s) 86, 401, 676, 953
BccI CCATC 6 cut(s) 398, 557, 590, 827, 988, 1049
BceAI ACGGC 4 cut(s) 62, 112, 212, 270
BciT130I CCWGG 4 cut(s) 6, 20, 224, 974
BclI TGATCA 2 cut(s) 808, 1285
BcnI CCSGG 4 cut(s) 166, 167, 208, 946
BcoDI GTCTC 2 cut(s) 258, 623
BfaI CTAG 1 cut(s) 38
BfmI CTRYAG 4 cut(s) 387, 770, 936, 1245
BfoI RGCGCY 1 cut(s) 111
BfuAI ACCTGC 2 cut(s) 451, 998
BisI GCNGC 5 cut(s) 75, 390, 690, 942, 1005
BlsI GCNGC 5 cut(s) 76, 391, 691, 943, 1006
Bme1390I CCNGG 8 cut(s) 6, 20, 166, 167, 208, 224, 946, 974
Bme18I GGWCC 2 cut(s) 169, 293
BmeT110I CYCGRG 3 cut(s) 165, 269, 486
BmgT120I GGNCC 6 cut(s) 136, 169, 259, 274, 293, 1058
BmiI GGNNCC 8 cut(s) 16, 170, 171, 260, 480, 750, 978, 1003
BmrFI CCNGG 8 cut(s) 6, 20, 166, 167, 208, 224, 946, 974
BmrI ACTGGG 2 cut(s) 20, 747
BmsI GCATC 2 cut(s) 47, 380
BmuI ACTGGG 2 cut(s) 20, 747
BpmI CTGGAG 1 cut(s) 525
BpuMI CCSGG 4 cut(s) 166, 167, 208, 946
BsaBI GATNNNNATC 1 cut(s) 1290
BsaHI GRCGYC 1 cut(s) 150
BsaI GGTCTC 1 cut(s) 258
BsaJI CCNNGG 8 cut(s) 18, 103, 139, 165, 222, 286, 323, 720
BsaWI WCCGGW 1 cut(s) 374
BsaXI ACNNNNNCTCC 4 cut(s) 447, 477, 1113, 1143
Bsc4I CCNNNNNNNGG 5 cut(s) 11, 145, 223, 250, 961
Bse1I ACTGG 3 cut(s) 15, 645, 753
Bse3DI GCAATG 3 cut(s) 792, 865, 1137
Bse8I GATNNNNATC 1 cut(s) 1290
BseBI CCWGG 4 cut(s) 6, 20, 224, 974
BseDI CCNNGG 8 cut(s) 18, 103, 139, 165, 222, 286, 323, 720
BseGI GGATG 4 cut(s) 371, 568, 898, 1060
BseJI GATNNNNATC 1 cut(s) 1290
BseLI CCNNNNNNNGG 5 cut(s) 11, 145, 223, 250, 961
BseMI GCAATG 3 cut(s) 792, 865, 1137
BseMII CTCAG 3 cut(s) 549, 836, 1055
BseNI ACTGG 3 cut(s) 15, 645, 753
BseRI GAGGAG 1 cut(s) 1014
BseX3I CGGCCG 1 cut(s) 283
BseXI GCAGC 4 cut(s) 86, 401, 676, 953
BseYI CCCAGC 1 cut(s) 276
Bsh1285I CGRYCG 1 cut(s) 286
BsiEI CGRYCG 1 cut(s) 286
BsiHKCI CYCGRG 3 cut(s) 165, 269, 486
BsiSI CCGG 4 cut(s) 166, 207, 375, 945
BslFI GGGAC 3 cut(s) 107, 155, 850
BslI CCNNNNNNNGG 5 cut(s) 11, 145, 223, 250, 961
BsmAI GTCTC 2 cut(s) 258, 623
BsmFI GGGAC 3 cut(s) 107, 155, 850
BsmI GAATGC 1 cut(s) 1132
Bso31I GGTCTC 1 cut(s) 258
BsoBI CYCGRG 3 cut(s) 165, 269, 486
Bsp1286I GDGCHC 1 cut(s) 162
Bsp143I GATC 4 cut(s) 14, 343, 808, 1285
Bsp19I CCATGG 1 cut(s) 103
BspACI CCGC 2 cut(s) 204, 1005
BspCNI CTCAG 3 cut(s) 550, 837, 1054
BspHI TCATGA 1 cut(s) 81
BspLI GGNNCC 8 cut(s) 16, 170, 171, 260, 480, 750, 978, 1003
BspMAI CTGCAG 1 cut(s) 391
BspMI ACCTGC 2 cut(s) 451, 998
BspPI GGATC 3 cut(s) 9, 22, 351
BspTNI GGTCTC 1 cut(s) 258
BsrDI GCAATG 3 cut(s) 792, 865, 1137
BsrI ACTGG 3 cut(s) 15, 645, 753
BssECI CCNNGG 8 cut(s) 18, 103, 139, 165, 222, 286, 323, 720
BssMI GATC 4 cut(s) 14, 343, 808, 1285
BssNI GRCGYC 1 cut(s) 150
BssT1I CCWWGG 4 cut(s) 103, 139, 323, 720
Bst2UI CCWGG 4 cut(s) 6, 20, 224, 974
Bst4CI ACNGT 2 cut(s) 669, 774
Bst6I CTCTTC 1 cut(s) 874
BstACI GRCGYC 1 cut(s) 150
BstC8I GCNNGC 4 cut(s) 119, 245, 443, 543
BstDEI CTNAG 4 cut(s) 519, 558, 845, 1041
BstDSI CCRYGG 2 cut(s) 103, 286
BstF5I GGATG 4 cut(s) 371, 568, 898, 1060
BstH2I RGCGCY 1 cut(s) 111
BstHHI GCGC 1 cut(s) 110
BstKTI GATC 4 cut(s) 17, 346, 811, 1288
BstMAI GTCTC 2 cut(s) 258, 623
BstMBI GATC 4 cut(s) 14, 343, 808, 1285
BstMCI CGRYCG 1 cut(s) 286
BstMWI GCNNNNNNNGC 4 cut(s) 105, 114, 442, 855
BstNI CCWGG 4 cut(s) 6, 20, 224, 974
BstNSI RCATGY 3 cut(s) 545, 736, 801
BstSCI CCNGG 8 cut(s) 4, 18, 164, 165, 206, 222, 944, 972
BstSFI CTRYAG 4 cut(s) 387, 770, 936, 1245
BstV1I GCAGC 4 cut(s) 86, 401, 676, 953
BstX2I RGATCY 1 cut(s) 14
BstXI CCANNNNNNTGG 1 cut(s) 744
BstYI RGATCY 1 cut(s) 14
BstZI CGGCCG 1 cut(s) 283
BtgI CCRYGG 2 cut(s) 103, 286
BtsCI GGATG 4 cut(s) 371, 568, 898, 1060
BtsIMutI CAGTG 1 cut(s) 779
BveI ACCTGC 2 cut(s) 451, 998
Cac8I GCNNGC 4 cut(s) 119, 245, 443, 543
CciI TCATGA 1 cut(s) 81
CfoI GCGC 1 cut(s) 110
Cfr13I GGNCC 6 cut(s) 136, 169, 259, 274, 293, 1058
Cfr9I CCCGGG 1 cut(s) 165
CseI GACGC 4 cut(s) 23, 158, 187, 847
CviAII CATG 8 cut(s) 61, 82, 104, 466, 542, 733, 798, 1020
DdeI CTNAG 4 cut(s) 519, 558, 845, 1041
DpnI GATC 4 cut(s) 16, 345, 810, 1287
DpnII GATC 4 cut(s) 14, 343, 808, 1285
EaeI YGGCCR 5 cut(s) 225, 283, 439, 717, 952
EagI CGGCCG 1 cut(s) 283
Eam1104I CTCTTC 1 cut(s) 874
EarI CTCTTC 1 cut(s) 874
EclXI CGGCCG 1 cut(s) 283
Eco130I CCWWGG 4 cut(s) 103, 139, 323, 720
Eco24I GRGCYC 1 cut(s) 162
Eco31I GGTCTC 1 cut(s) 258
Eco47I GGWCC 2 cut(s) 169, 293
Eco52I CGGCCG 1 cut(s) 283
Eco88I CYCGRG 3 cut(s) 165, 269, 486
EcoO109I RGGNCCY 2 cut(s) 169, 293
EcoRII CCWGG 4 cut(s) 4, 18, 222, 972
EcoT14I CCWWGG 4 cut(s) 103, 139, 323, 720
EcoT22I ATGCAT 1 cut(s) 62
EcoT38I GRGCYC 1 cut(s) 162
ErhI CCWWGG 4 cut(s) 103, 139, 323, 720
FaeI CATG 8 cut(s) 64, 85, 107, 469, 545, 736, 801, 1023
FaqI GGGAC 3 cut(s) 107, 155, 850
FatI CATG 8 cut(s) 60, 81, 103, 465, 541, 732, 797, 1019
FbaI TGATCA 2 cut(s) 808, 1285
FblI GTMKAC 1 cut(s) 789
Fnu4HI GCNGC 5 cut(s) 75, 390, 690, 942, 1005
FokI GGATG 4 cut(s) 358, 575, 905, 1067
FriOI GRGCYC 1 cut(s) 162
Fsp4HI GCNGC 5 cut(s) 75, 390, 690, 942, 1005
FspBI CTAG 1 cut(s) 38
GlaI GCGC 1 cut(s) 109
GluI GCNGC 5 cut(s) 75, 390, 690, 942, 1005
GsaI CCCAGC 1 cut(s) 280
GsuI CTGGAG 1 cut(s) 525
HaeII RGCGCY 1 cut(s) 111
HapII CCGG 4 cut(s) 166, 207, 375, 945
HgaI GACGC 4 cut(s) 23, 158, 187, 847
HhaI GCGC 1 cut(s) 110
Hin1I GRCGYC 1 cut(s) 150
Hin1II CATG 8 cut(s) 64, 85, 107, 469, 545, 736, 801, 1023
Hin6I GCGC 1 cut(s) 108
HinP1I GCGC 1 cut(s) 108
HindIII AAGCTT 1 cut(s) 919
HinfI GANTC 3 cut(s) 620, 1030, 1241
HpaII CCGG 4 cut(s) 166, 207, 375, 945
HphI GGTGA 4 cut(s) 34, 184, 193, 842
Hpy166II GTNNAC 2 cut(s) 148, 790
Hpy188I TCNGA 5 cut(s) 54, 238, 988, 1035, 1153
Hpy188III TCNNGA 5 cut(s) 82, 185, 486, 662, 806
Hpy8I GTNNAC 2 cut(s) 148, 790
HpyAV CCTTC 8 cut(s) 183, 233, 306, 492, 704, 1079, 1088, 1185
HpyCH4III ACNGT 2 cut(s) 669, 774
HpyCH4V TGCA 8 cut(s) 60, 365, 371, 389, 527, 551, 797, 1132
HpyF10VI GCNNNNNNNGC 4 cut(s) 105, 114, 442, 855
HpyF3I CTNAG 4 cut(s) 519, 558, 845, 1041
Hsp92I GRCGYC 1 cut(s) 150
Hsp92II CATG 8 cut(s) 64, 85, 107, 469, 545, 736, 801, 1023
HspAI GCGC 1 cut(s) 108
KflI GGGWCCC 1 cut(s) 169
Ksp22I TGATCA 2 cut(s) 808, 1285
Kzo9I GATC 4 cut(s) 14, 343, 808, 1285
LmnI GCTCC 3 cut(s) 855, 1001, 1214
Lsp1109I GCAGC 4 cut(s) 86, 401, 676, 953
LweI GCATC 2 cut(s) 47, 380
MaeI CTAG 1 cut(s) 38
MaeIII GTNAC 3 cut(s) 199, 229, 331
MalI GATC 4 cut(s) 16, 345, 810, 1287
MboI GATC 4 cut(s) 14, 343, 808, 1285
MboII GAAGA 3 cut(s) 37, 891, 1286
MflI RGATCY 1 cut(s) 14
MhlI GDGCHC 1 cut(s) 162
MlsI TGGCCA 3 cut(s) 441, 719, 954
MluCI AATT 4 cut(s) 819, 966, 1230, 1302
MluNI TGGCCA 3 cut(s) 441, 719, 954
MlyI GAGTC 1 cut(s) 1235
MmeI TCCRAC 1 cut(s) 1007
Mox20I TGGCCA 3 cut(s) 441, 719, 954
Mph1103I ATGCAT 1 cut(s) 62
MroXI GAANNNNTTC 1 cut(s) 1068
MscI TGGCCA 3 cut(s) 441, 719, 954
MseI TTAA 4 cut(s) 131, 615, 818, 1301
Msp20I TGGCCA 3 cut(s) 441, 719, 954
MspI CCGG 4 cut(s) 166, 207, 375, 945
MspR9I CCNGG 8 cut(s) 6, 20, 166, 167, 208, 224, 946, 974
Mva1269I GAATGC 1 cut(s) 1132
MvaI CCWGG 4 cut(s) 6, 20, 224, 974
MwoI GCNNNNNNNGC 4 cut(s) 105, 114, 442, 855
NciI CCSGG 4 cut(s) 166, 167, 208, 946
NcoI CCATGG 1 cut(s) 103
NdeII GATC 4 cut(s) 14, 343, 808, 1285
NlaIII CATG 8 cut(s) 64, 85, 107, 469, 545, 736, 801, 1023
NlaIV GGNNCC 8 cut(s) 16, 170, 171, 260, 480, 750, 978, 1003
NmeAIII GCCGAG 3 cut(s) 261, 287, 718
NmuCI GTSAC 2 cut(s) 199, 229
NsiI ATGCAT 1 cut(s) 62
NspI RCATGY 3 cut(s) 545, 736, 801
PaeI GCATGC 1 cut(s) 545
PaeR7I CTCGAG 1 cut(s) 269
PagI TCATGA 1 cut(s) 81
PaqCI CACCTGC 1 cut(s) 998
PctI GAATGC 1 cut(s) 1132
PdmI GAANNNNTTC 1 cut(s) 1068
PfeI GAWTC 2 cut(s) 620, 1030
PflMI CCANNNNNTGG 2 cut(s) 223, 961
PkrI GCNGC 5 cut(s) 76, 391, 691, 943, 1006
PleI GAGTC 1 cut(s) 1235
PpsI GAGTC 1 cut(s) 1235
PpuMI RGGWCCY 2 cut(s) 169, 293
PshBI ATTAAT 1 cut(s) 818
Psp5II RGGWCCY 2 cut(s) 169, 293
Psp6I CCWGG 4 cut(s) 4, 18, 222, 972
PspFI CCCAGC 1 cut(s) 276
PspGI CCWGG 4 cut(s) 4, 18, 222, 972
PspN4I GGNNCC 8 cut(s) 16, 170, 171, 260, 480, 750, 978, 1003
PspPI GGNCC 6 cut(s) 136, 169, 259, 274, 293, 1058
PspPPI RGGWCCY 2 cut(s) 169, 293
PspXI VCTCGAGB 1 cut(s) 269
PstI CTGCAG 1 cut(s) 391
PsuI RGATCY 1 cut(s) 14
SaqAI TTAA 4 cut(s) 131, 615, 818, 1301
SatI GCNGC 5 cut(s) 75, 390, 690, 942, 1005
Sau3AI GATC 4 cut(s) 14, 343, 808, 1285
Sau96I GGNCC 6 cut(s) 136, 169, 259, 274, 293, 1058
SchI GAGTC 1 cut(s) 1235
ScrFI CCNGG 8 cut(s) 6, 20, 166, 167, 208, 224, 946, 974
SduI GDGCHC 1 cut(s) 162
SfaNI GCATC 2 cut(s) 47, 380
SfcI CTRYAG 4 cut(s) 387, 770, 936, 1245
Sfr274I CTCGAG 1 cut(s) 269
SinI GGWCC 2 cut(s) 169, 293
SlaI CTCGAG 1 cut(s) 269
SmaI CCCGGG 1 cut(s) 167
SmlI CTYRAG 1 cut(s) 269
SmoI CTYRAG 1 cut(s) 269
SphI GCATGC 1 cut(s) 545
Sse9I AATT 4 cut(s) 819, 966, 1230, 1302
SsiI CCGC 2 cut(s) 204, 1005
SspI AATATT 1 cut(s) 1069
SspMI CTAG 1 cut(s) 38
StyD4I CCNGG 8 cut(s) 4, 18, 164, 165, 206, 222, 944, 972
StyI CCWWGG 4 cut(s) 103, 139, 323, 720
TaaI ACNGT 2 cut(s) 669, 774
TaqI TCGA 2 cut(s) 270, 671
TasI AATT 4 cut(s) 819, 966, 1230, 1302
TauI GCSGC 1 cut(s) 1007
TfiI GAWTC 2 cut(s) 620, 1030
Tru1I TTAA 4 cut(s) 131, 615, 818, 1301
Tru9I TTAA 4 cut(s) 131, 615, 818, 1301
TscAI CASTG 1 cut(s) 779
TseFI GTSAC 2 cut(s) 199, 229
TseI GCWGC 4 cut(s) 74, 389, 689, 941
Tsp45I GTSAC 2 cut(s) 199, 229
TspDTI ATGAA 4 cut(s) 333, 666, 804, 1233
TspGWI ACGGA 1 cut(s) 914
TspMI CCCGGG 1 cut(s) 165
TspRI CASTG 1 cut(s) 779
Van91I CCANNNNNTGG 2 cut(s) 223, 961
VpaK11BI GGWCC 2 cut(s) 169, 293
VspI ATTAAT 1 cut(s) 818
XceI RCATGY 3 cut(s) 545, 736, 801
XcmI CCANNNNNNNNNTGG 2 cut(s) 130, 284
XhoI CTCGAG 1 cut(s) 269
XmaI CCCGGG 1 cut(s) 165
XmiI GTMKAC 1 cut(s) 789
XmnI GAANNNNTTC 1 cut(s) 1068
XspI CTAG 1 cut(s) 38
Zsp2I ATGCAT 1 cut(s) 62
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.