RLG00000017062

Regulator of chromosome condensation (RCC1) repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0000878
Physical Location & Seq
Forward (+)
12481141 .. 12483145
2005 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000017062

Sequence Viewer

Length: 1326 bp
ATGCCAGGACGGGGATCGCTGGTGAAGAACCAGCGTCTAGTAAACGCGGTTGTCCAAAGGTTTCAGAGATGCATGACCACAACAACAGCCGTCATGAGCTTCGGGGACGGCAACCATGGCGCTCTGGGCTTGCCCACTTCCTTAATGGGCCTTGGTGTGGACTCCTACGAGCCCACCCGGGTCCCTTCTCTGCCTACTGACATCACCCGCGTCACCGCCGGGCATTACCACTCCCTGGCCGTCACTTCTGAAGGCCAGCTTTGGGCTTGGGGCCGAGACCTCGAGGCCCAGCTCGGCCGTGGAGGTCCTTCTCCCAGAGATTCGTGGAATGAACCAAGGAGAGTAACAGGATTGGATCAAGTAAATGTTTGTTCTGCATTTGCATCCGGTGTCATTTCTGCGGCCATTGGAGATGATGGTTCTTTGTGGGTTTGGGGAAAGTCAAAGCGTGGCCAGCTTGGTCTGGGAGAGCAAGTCATGAAGGCTCTGCTGCCTTCCCGAGTTGAAGCACTTGCTGGAGAAAAAATAACTAAGGTTGCACTTGGTTGGGGGCATGCTCTTGCGTTGACTGAGGATGGAAAGTTATTTGGTTGGGGTTATTTTGCTGATGGTAGGTTAGGAAGCATTAAAGAATCTTTGGAGACATCTCAACTGGAATCTACTTCCAATAATCAAGAACTGTCGAGTTCGTCTTTGGAAGCTGCTGAGAAGCTGGTTTTAGGAGGAATGGCCAAGGAGAATAATATGCCAATAGTTTGGGAACCCAGTTTAGTGAGGGAACTACAGTGTATTGAAGTTGTAGACATTGCATGTGGTCTTGATCATTCATTAACTCTTTGTGGTGATGGGACGCTACTGAGCTCTGGGAGCAATGTATATGGTCAGTTGGGAAGAGAAAAACAGGATGTAGGATTGTTTCCTGTTGATATAAGCTTCCGTCCAATGTCTATAGCAGCCGGGCTTGGCCATTCTTTGGCAATTTGCCAGGTTCCATCATCAGATGTTATAGGAGGAGCCGCAGGTGTCATTACATGGGGTTGGAATCAGACCTCTCAGCTTGGTAGGGATGGGCCAGAAAATATTCCATTAGGAGTTGAAGGCTTGGAAGGGGAAACTCCTGTATCAGTAGCAGGAGGTCGTGTGCATTCCATTGCTCTAACATCAAAGAGAGAAGTGTGGGTTTGGGGTAGTGGTAAGAATGGAAGGCTTGGGTTAGGAAGCTCCTGTGATGAACCTGAACCAATTTTGCTGGACTCTGTAGAGGGTTGTGATGTTTTGCAAGCTGTGTCAGGGTTTGATCATAATCTTCTCTTAATTGCCGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RCC1 RCC1_2

Protein Analysis

442

Amino Acids

46.57

Weight (kDa)

5.05

Isoelectric Point (pI)

37.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_RLD PF25390 31 - 436 4.9e-80 RCC1-like domain
RCC1 PF00415 33 - 81 5.6e-08 Regulator of chromosome condensation (RCC1) repeat
RCC1_2 PF13540 68 - 93 5.7e-07 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 84 - 136 3.1e-06 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 139 - 189 1.9e-12 Regulator of chromosome condensation (RCC1) repeat
RCC1_2 PF13540 176 - 203 1.3e-08 Regulator of chromosome condensation (RCC1) repeat
RCC1_2 PF13540 266 - 295 6.4e-07 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 342 - 386 1.7e-07 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 391 - 438 3e-08 Regulator of chromosome condensation (RCC1) repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1010
AbsI CCTCGAGG 1 cut(s) 281
Acc36I ACCTGC 1 cut(s) 1010
AccB7I CCANNNNNTGG 2 cut(s) 235, 973
AccI GTMKAC 1 cut(s) 801
AccII CGCG 2 cut(s) 47, 210
AciI CCGC 5 cut(s) 47, 208, 216, 401, 1017
AclWI GGATC 2 cut(s) 22, 363
AcoI YGGCCR 6 cut(s) 237, 295, 402, 451, 729, 964
AcuI CTGAAG 1 cut(s) 270
AfiI CCNNNNNNNGG 5 cut(s) 11, 157, 235, 262, 973
AgsI TTSAA 3 cut(s) 506, 794, 1097
AjnI CCWGG 3 cut(s) 4, 234, 984
Alw21I GWGCWC 1 cut(s) 863
Alw26I GTCTC 2 cut(s) 270, 635
AlwI GGATC 2 cut(s) 22, 363
Ama87I CYCGRG 3 cut(s) 177, 281, 498
ApeKI GCWGC 3 cut(s) 490, 701, 953
Asp700I GAANNNNTTC 1 cut(s) 1080
AspLEI GCGC 1 cut(s) 122
AspS9I GGNCC 6 cut(s) 148, 181, 271, 286, 305, 1070
AsuC2I CCSGG 4 cut(s) 178, 179, 220, 958
AsuHPI GGTGA 4 cut(s) 34, 196, 205, 854
AvaI CYCGRG 3 cut(s) 177, 281, 498
AvaII GGWCC 2 cut(s) 181, 305
BaeI ACNNNNGTAYC 2 cut(s) 1104, 1137
BalI TGGCCA 3 cut(s) 453, 731, 966
BanII GRGCYC 2 cut(s) 174, 863
Bbv12I GWGCWC 1 cut(s) 863
BbvI GCAGC 3 cut(s) 477, 688, 965
BccI CCATC 6 cut(s) 410, 569, 602, 839, 1000, 1061
BceAI ACGGC 4 cut(s) 74, 124, 224, 282
BciT130I CCWGG 3 cut(s) 6, 236, 986
BclI TGATCA 2 cut(s) 820, 1297
BcnI CCSGG 4 cut(s) 178, 179, 220, 958
BcoDI GTCTC 2 cut(s) 270, 635
BfaI CTAG 1 cut(s) 38
BfmI CTRYAG 3 cut(s) 782, 948, 1257
BfoI RGCGCY 1 cut(s) 123
BfuAI ACCTGC 1 cut(s) 1010
BisI GCNGC 5 cut(s) 402, 491, 702, 954, 1017
BlsI GCNGC 5 cut(s) 403, 492, 703, 955, 1018
Bme1390I CCNGG 7 cut(s) 6, 178, 179, 220, 236, 958, 986
Bme18I GGWCC 2 cut(s) 181, 305
BmeT110I CYCGRG 3 cut(s) 177, 281, 498
BmgT120I GGNCC 6 cut(s) 148, 181, 271, 286, 305, 1070
BmiI GGNNCC 6 cut(s) 182, 183, 272, 762, 990, 1015
BmrFI CCNGG 7 cut(s) 6, 178, 179, 220, 236, 958, 986
BmrI ACTGGG 1 cut(s) 759
BmsI GCATC 2 cut(s) 59, 392
BmuI ACTGGG 1 cut(s) 759
BpmI CTGGAG 1 cut(s) 537
BpuMI CCSGG 4 cut(s) 178, 179, 220, 958
BsaBI GATNNNNATC 1 cut(s) 1302
BsaI GGTCTC 1 cut(s) 270
BsaJI CCNNGG 7 cut(s) 115, 151, 177, 234, 298, 335, 732
BsaWI WCCGGW 1 cut(s) 386
BsaXI ACNNNNNCTCC 4 cut(s) 459, 489, 1125, 1155
Bsc4I CCNNNNNNNGG 5 cut(s) 11, 157, 235, 262, 973
Bse1I ACTGG 2 cut(s) 657, 765
Bse3DI GCAATG 3 cut(s) 804, 877, 1149
Bse8I GATNNNNATC 1 cut(s) 1302
BseBI CCWGG 3 cut(s) 6, 236, 986
BseDI CCNNGG 7 cut(s) 115, 151, 177, 234, 298, 335, 732
BseGI GGATG 4 cut(s) 383, 580, 910, 1072
BseJI GATNNNNATC 1 cut(s) 1302
BseLI CCNNNNNNNGG 5 cut(s) 11, 157, 235, 262, 973
BseMI GCAATG 3 cut(s) 804, 877, 1149
BseMII CTCAG 4 cut(s) 561, 696, 848, 1067
BseNI ACTGG 2 cut(s) 657, 765
BseRI GAGGAG 1 cut(s) 1026
BseX3I CGGCCG 1 cut(s) 295
BseXI GCAGC 3 cut(s) 477, 688, 965
BseYI CCCAGC 1 cut(s) 288
Bsh1236I CGCG 2 cut(s) 47, 210
Bsh1285I CGRYCG 1 cut(s) 298
BsiEI CGRYCG 1 cut(s) 298
BsiHKAI GWGCWC 1 cut(s) 863
BsiHKCI CYCGRG 3 cut(s) 177, 281, 498
BsiSI CCGG 4 cut(s) 178, 219, 387, 957
BslFI GGGAC 3 cut(s) 119, 167, 862
BslI CCNNNNNNNGG 5 cut(s) 11, 157, 235, 262, 973
BsmAI GTCTC 2 cut(s) 270, 635
BsmFI GGGAC 3 cut(s) 119, 167, 862
BsmI GAATGC 1 cut(s) 1144
Bso31I GGTCTC 1 cut(s) 270
BsoBI CYCGRG 3 cut(s) 177, 281, 498
Bsp1286I GDGCHC 2 cut(s) 174, 863
Bsp143I GATC 4 cut(s) 14, 355, 820, 1297
Bsp19I CCATGG 1 cut(s) 115
BspACI CCGC 5 cut(s) 47, 208, 216, 401, 1017
BspCNI CTCAG 4 cut(s) 562, 697, 849, 1066
BspFNI CGCG 2 cut(s) 47, 210
BspHI TCATGA 2 cut(s) 93, 477
BspLI GGNNCC 6 cut(s) 182, 183, 272, 762, 990, 1015
BspMI ACCTGC 1 cut(s) 1010
BspPI GGATC 2 cut(s) 22, 363
BspTNI GGTCTC 1 cut(s) 270
BsrDI GCAATG 3 cut(s) 804, 877, 1149
BsrI ACTGG 2 cut(s) 657, 765
BssECI CCNNGG 7 cut(s) 115, 151, 177, 234, 298, 335, 732
BssMI GATC 4 cut(s) 14, 355, 820, 1297
BssT1I CCWWGG 4 cut(s) 115, 151, 335, 732
Bst2UI CCWGG 3 cut(s) 6, 236, 986
Bst4CI ACNGT 2 cut(s) 681, 786
Bst6I CTCTTC 1 cut(s) 886
BstC8I GCNNGC 5 cut(s) 131, 257, 455, 555, 1281
BstDEI CTNAG 5 cut(s) 531, 570, 705, 857, 1053
BstDSI CCRYGG 2 cut(s) 115, 298
BstF5I GGATG 4 cut(s) 383, 580, 910, 1072
BstFNI CGCG 2 cut(s) 47, 210
BstH2I RGCGCY 1 cut(s) 123
BstHHI GCGC 1 cut(s) 122
BstKTI GATC 4 cut(s) 17, 358, 823, 1300
BstMAI GTCTC 2 cut(s) 270, 635
BstMBI GATC 4 cut(s) 14, 355, 820, 1297
BstMCI CGRYCG 1 cut(s) 298
BstMWI GCNNNNNNNGC 4 cut(s) 117, 126, 454, 867
BstNI CCWGG 3 cut(s) 6, 236, 986
BstNSI RCATGY 2 cut(s) 557, 813
BstSCI CCNGG 7 cut(s) 4, 176, 177, 218, 234, 956, 984
BstSFI CTRYAG 3 cut(s) 782, 948, 1257
BstUI CGCG 2 cut(s) 47, 210
BstV1I GCAGC 3 cut(s) 477, 688, 965
BstXI CCANNNNNNTGG 1 cut(s) 756
BstZI CGGCCG 1 cut(s) 295
BtgI CCRYGG 2 cut(s) 115, 298
BtsCI GGATG 4 cut(s) 383, 580, 910, 1072
BtsIMutI CAGTG 1 cut(s) 791
BveI ACCTGC 1 cut(s) 1010
Cac8I GCNNGC 5 cut(s) 131, 257, 455, 555, 1281
CciI TCATGA 2 cut(s) 93, 477
CfoI GCGC 1 cut(s) 122
Cfr13I GGNCC 6 cut(s) 148, 181, 271, 286, 305, 1070
Cfr9I CCCGGG 1 cut(s) 177
CseI GACGC 3 cut(s) 23, 199, 859
CviAII CATG 7 cut(s) 73, 94, 116, 478, 554, 810, 1032
DdeI CTNAG 5 cut(s) 531, 570, 705, 857, 1053
DpnI GATC 4 cut(s) 16, 357, 822, 1299
DpnII GATC 4 cut(s) 14, 355, 820, 1297
EaeI YGGCCR 6 cut(s) 237, 295, 402, 451, 729, 964
EagI CGGCCG 1 cut(s) 295
Eam1104I CTCTTC 1 cut(s) 886
EarI CTCTTC 1 cut(s) 886
Ecl136II GAGCTC 1 cut(s) 861
EclXI CGGCCG 1 cut(s) 295
Eco130I CCWWGG 4 cut(s) 115, 151, 335, 732
Eco24I GRGCYC 2 cut(s) 174, 863
Eco31I GGTCTC 1 cut(s) 270
Eco47I GGWCC 2 cut(s) 181, 305
Eco52I CGGCCG 1 cut(s) 295
Eco53kI GAGCTC 1 cut(s) 861
Eco57I CTGAAG 1 cut(s) 270
Eco88I CYCGRG 3 cut(s) 177, 281, 498
EcoICRI GAGCTC 1 cut(s) 861
EcoO109I RGGNCCY 2 cut(s) 181, 305
EcoRII CCWGG 3 cut(s) 4, 234, 984
EcoT14I CCWWGG 4 cut(s) 115, 151, 335, 732
EcoT22I ATGCAT 1 cut(s) 74
EcoT38I GRGCYC 2 cut(s) 174, 863
ErhI CCWWGG 4 cut(s) 115, 151, 335, 732
FaeI CATG 7 cut(s) 76, 97, 119, 481, 557, 813, 1035
FalI AAGNNNNNCTT 2 cut(s) 243, 275
FaqI GGGAC 3 cut(s) 119, 167, 862
FatI CATG 7 cut(s) 72, 93, 115, 477, 553, 809, 1031
FauI CCCGC 1 cut(s) 215
FbaI TGATCA 2 cut(s) 820, 1297
FblI GTMKAC 1 cut(s) 801
Fnu4HI GCNGC 5 cut(s) 402, 491, 702, 954, 1017
FokI GGATG 4 cut(s) 370, 587, 917, 1079
FriOI GRGCYC 2 cut(s) 174, 863
Fsp4HI GCNGC 5 cut(s) 402, 491, 702, 954, 1017
FspBI CTAG 1 cut(s) 38
GlaI GCGC 1 cut(s) 121
GluI GCNGC 5 cut(s) 402, 491, 702, 954, 1017
GsaI CCCAGC 1 cut(s) 292
GsuI CTGGAG 1 cut(s) 537
HaeII RGCGCY 1 cut(s) 123
HapII CCGG 4 cut(s) 178, 219, 387, 957
HgaI GACGC 3 cut(s) 23, 199, 859
HhaI GCGC 1 cut(s) 122
Hin1II CATG 7 cut(s) 76, 97, 119, 481, 557, 813, 1035
Hin6I GCGC 1 cut(s) 120
HinP1I GCGC 1 cut(s) 120
HincII GTYRAC 1 cut(s) 567
HindII GTYRAC 1 cut(s) 567
HindIII AAGCTT 1 cut(s) 931
HinfI GANTC 6 cut(s) 161, 320, 632, 656, 1042, 1253
HpaII CCGG 4 cut(s) 178, 219, 387, 957
HphI GGTGA 4 cut(s) 34, 196, 205, 854
Hpy166II GTNNAC 4 cut(s) 43, 160, 567, 802
Hpy188I TCNGA 4 cut(s) 66, 250, 1000, 1047
Hpy188III TCNNGA 5 cut(s) 94, 478, 498, 674, 818
Hpy8I GTNNAC 4 cut(s) 43, 160, 567, 802
HpyAV CCTTC 8 cut(s) 195, 245, 318, 475, 504, 1091, 1100, 1197
HpyCH4III ACNGT 2 cut(s) 681, 786
HpyCH4V TGCA 7 cut(s) 72, 377, 383, 539, 809, 1144, 1279
HpyF10VI GCNNNNNNNGC 4 cut(s) 117, 126, 454, 867
HpyF3I CTNAG 5 cut(s) 531, 570, 705, 857, 1053
Hsp92II CATG 7 cut(s) 76, 97, 119, 481, 557, 813, 1035
HspAI GCGC 1 cut(s) 120
KflI GGGWCCC 1 cut(s) 181
Ksp22I TGATCA 2 cut(s) 820, 1297
Kzo9I GATC 4 cut(s) 14, 355, 820, 1297
LmnI GCTCC 3 cut(s) 867, 1013, 1226
Lsp1109I GCAGC 3 cut(s) 477, 688, 965
LweI GCATC 2 cut(s) 59, 392
MaeI CTAG 1 cut(s) 38
MaeIII GTNAC 3 cut(s) 211, 241, 343
MalI GATC 4 cut(s) 16, 357, 822, 1299
MboI GATC 4 cut(s) 14, 355, 820, 1297
MboII GAAGA 3 cut(s) 37, 903, 1298
MhlI GDGCHC 2 cut(s) 174, 863
MlsI TGGCCA 3 cut(s) 453, 731, 966
MluCI AATT 3 cut(s) 978, 1242, 1314
MluNI TGGCCA 3 cut(s) 453, 731, 966
MlyI GAGTC 2 cut(s) 155, 1247
MmeI TCCRAC 1 cut(s) 1019
Mox20I TGGCCA 3 cut(s) 453, 731, 966
Mph1103I ATGCAT 1 cut(s) 74
MroXI GAANNNNTTC 1 cut(s) 1080
MscI TGGCCA 3 cut(s) 453, 731, 966
MseI TTAA 4 cut(s) 143, 627, 830, 1313
Msp20I TGGCCA 3 cut(s) 453, 731, 966
MspI CCGG 4 cut(s) 178, 219, 387, 957
MspR9I CCNGG 7 cut(s) 6, 178, 179, 220, 236, 958, 986
Mva1269I GAATGC 1 cut(s) 1144
MvaI CCWGG 3 cut(s) 6, 236, 986
MvnI CGCG 2 cut(s) 47, 210
MwoI GCNNNNNNNGC 4 cut(s) 117, 126, 454, 867
NciI CCSGG 4 cut(s) 178, 179, 220, 958
NcoI CCATGG 1 cut(s) 115
NdeII GATC 4 cut(s) 14, 355, 820, 1297
NlaIII CATG 7 cut(s) 76, 97, 119, 481, 557, 813, 1035
NlaIV GGNNCC 6 cut(s) 182, 183, 272, 762, 990, 1015
NmeAIII GCCGAG 2 cut(s) 273, 299
NmuCI GTSAC 2 cut(s) 211, 241
NsiI ATGCAT 1 cut(s) 74
NspI RCATGY 2 cut(s) 557, 813
PaeI GCATGC 1 cut(s) 557
PaeR7I CTCGAG 1 cut(s) 281
PagI TCATGA 2 cut(s) 93, 477
PaqCI CACCTGC 1 cut(s) 1010
PctI GAATGC 1 cut(s) 1144
PdmI GAANNNNTTC 1 cut(s) 1080
PfeI GAWTC 4 cut(s) 320, 632, 656, 1042
PflMI CCANNNNNTGG 2 cut(s) 235, 973
PkrI GCNGC 5 cut(s) 403, 492, 703, 955, 1018
PleI GAGTC 2 cut(s) 155, 1247
PpsI GAGTC 2 cut(s) 155, 1247
PpuMI RGGWCCY 2 cut(s) 181, 305
Psp124BI GAGCTC 1 cut(s) 863
Psp5II RGGWCCY 2 cut(s) 181, 305
Psp6I CCWGG 3 cut(s) 4, 234, 984
PspFI CCCAGC 1 cut(s) 288
PspGI CCWGG 3 cut(s) 4, 234, 984
PspN4I GGNNCC 6 cut(s) 182, 183, 272, 762, 990, 1015
PspPI GGNCC 6 cut(s) 148, 181, 271, 286, 305, 1070
PspPPI RGGWCCY 2 cut(s) 181, 305
PspXI VCTCGAGB 1 cut(s) 281
SacI GAGCTC 1 cut(s) 863
SaqAI TTAA 4 cut(s) 143, 627, 830, 1313
SatI GCNGC 5 cut(s) 402, 491, 702, 954, 1017
Sau3AI GATC 4 cut(s) 14, 355, 820, 1297
Sau96I GGNCC 6 cut(s) 148, 181, 271, 286, 305, 1070
SchI GAGTC 2 cut(s) 155, 1247
ScrFI CCNGG 7 cut(s) 6, 178, 179, 220, 236, 958, 986
SduI GDGCHC 2 cut(s) 174, 863
SfaNI GCATC 2 cut(s) 59, 392
SfcI CTRYAG 3 cut(s) 782, 948, 1257
Sfr274I CTCGAG 1 cut(s) 281
SinI GGWCC 2 cut(s) 181, 305
SlaI CTCGAG 1 cut(s) 281
SmaI CCCGGG 1 cut(s) 179
SmlI CTYRAG 1 cut(s) 281
SmoI CTYRAG 1 cut(s) 281
SphI GCATGC 1 cut(s) 557
Sse9I AATT 3 cut(s) 978, 1242, 1314
SsiI CCGC 5 cut(s) 47, 208, 216, 401, 1017
SspI AATATT 1 cut(s) 1081
SspMI CTAG 1 cut(s) 38
SstI GAGCTC 1 cut(s) 863
StyD4I CCNGG 7 cut(s) 4, 176, 177, 218, 234, 956, 984
StyI CCWWGG 4 cut(s) 115, 151, 335, 732
TaaI ACNGT 2 cut(s) 681, 786
TaqI TCGA 2 cut(s) 282, 683
TasI AATT 3 cut(s) 978, 1242, 1314
TauI GCSGC 2 cut(s) 404, 1019
TfiI GAWTC 4 cut(s) 320, 632, 656, 1042
Tru1I TTAA 4 cut(s) 143, 627, 830, 1313
Tru9I TTAA 4 cut(s) 143, 627, 830, 1313
TscAI CASTG 1 cut(s) 791
TseFI GTSAC 2 cut(s) 211, 241
TseI GCWGC 3 cut(s) 490, 701, 953
Tsp45I GTSAC 2 cut(s) 211, 241
TspDTI ATGAA 4 cut(s) 345, 494, 816, 1245
TspGWI ACGGA 1 cut(s) 926
TspMI CCCGGG 1 cut(s) 177
TspRI CASTG 1 cut(s) 791
Van91I CCANNNNNTGG 2 cut(s) 235, 973
VpaK11BI GGWCC 2 cut(s) 181, 305
XceI RCATGY 2 cut(s) 557, 813
XcmI CCANNNNNNNNNTGG 2 cut(s) 142, 296
XhoI CTCGAG 1 cut(s) 281
XmaI CCCGGG 1 cut(s) 177
XmiI GTMKAC 1 cut(s) 801
XmnI GAANNNNTTC 1 cut(s) 1080
XspI CTAG 1 cut(s) 38
Zsp2I ATGCAT 1 cut(s) 74
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.