pycom808g00780

Encoded by

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
tig00000808
OG0000917
Physical Location & Seq
Forward (+)
536528 .. 538246
1719 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom808g00780.1

Sequence Viewer

Length: 1719 bp
ATGGAAGAAATAGATTTGCTACCCAAAGTGAAGCTCTTTGATCAAACTCTGTCGAAGCAGTTTACCGACATAGAACGACAACTTGGAAAGATTATCTTGGGAGCTGAAACCACTTACAGCAAAAAAGAGGAAATGTACAAGGCCCTAGGTCTCATGAAACGAAATGTCGATCAGATAATTGAAGACTCAATCCATTTAGAGAAGAAAATGCCTACCCATGCACCCATCCAAGAGTCACAGGTGCATTGGTCTACAGTCGATCAGTCAGGACCAGATCAGAAGATGTCACAAGAGTGGTCACAACTGGGCCTGGAGGATAGAATTTATGAGACTCCTGCCATGGCTAATATAAGGAGGACTTATGAAGATCTTGAAAGCATGGATTTGAAGTTGTGCTTCCTCTCTTTCTCAATCTTCCCGGATGGTTCCGTGATGAAGAAGAGGCCTCTGATTTACTGGTGGATTGGCGAGGGCTTCATCACGTCAACCCAACAGAAGACAGCAGAAGAGGTAGGAGAAGATATATTTGAAAAACTTATGAGAAAGGGCTTGATTAATCAAACTGGCTCAAATGCATCCTCCTGTCCGTTTGTAAAAAGTTGTGTATTGAACCCTTGGATCCATCGTATGTTGATCTCCTTGGCAAGTGAAGCTAGGCTGTTTCACTTTGATTCAGGATGGCAAAGAATGCCATCTTATGATACCTTAACCTGTAGGCGTGCATGCTTAGTTTTTAATAATCAAATTCGTCAGGGTGATGATGGCCCAAAGGTGGACGATCTGTTGATTGTATTCAATGTCAATGTGCAATATCTTGGTTTGAAAAAAGAATGGCTCAATAAGTTGAAGAAGGTTGGGGTTCTTCAGCTTGGGCGCTGGCAGGACTCGGCTACTCATCATATTGAGGTGGATGATGAAGACTTTGTGAACCAAGAAGATGTGGGATTTCTAAAAGGTTTGGGGACTCAGAATAAGCACTTGAAGTATCTTAGCCTCAGAGGAATATCAAGAATTACCCAACTCCCTTCTTCTATCCTTAATCTTATAAGCCTTGAGATTCTGGATCTTCGAGCATGTCACAACTTAGAGACGATGCCTTCAGATATCTCCGCATTAAGGAAGCTCACCCATTTGGATATGTCGGAGTGCTACTTGCTTGAACGCATGCCAAAAGGGCTTGACAAACTCTCTTCGCTCCAAGTTCTGAAAGGTTTTCTCCTGGGGTCTTTGAAAAATAATCCCTGTAAACTTGGTGATCTTGCCAAGTTGAAGAATTTAAAGCGGCTCAGTATACACATGGGGAATGAGTCTGAAGTTCAAAAGGGGGAACTCAACAAGTTGAAGGACATTTCATCTCTTTGCCGCCTTAAAATATCTTGGGGTTTGGTTTCTTCCGTGTTAAAAGACCAAATTGCCAATGAATTCTCATTTCCGCCGAATTTGGAAAAATTGGACCTCCAAGGTATCCCCCTAGAAAACGTACCAAAATGGCTGAACCCGAGACAACTGGGAAATTTAAAGAAGCTGTACATAAGGGGTGGAGAACTTTCCAGCTTAGATCATGGGACCGGTGCTATGTGGAAGGTAGAAATCTTGCGTCTCAAGTACTTGGCGAAATGGGACATAGATTGGCCAAAGTTGAAGGGGTCTTTTCCTCGTCTGCAGCACTGGGATTTGGAGAAGTTCCACTGTCACGAGATTAAAAAGGACGTAAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

573

Amino Acids

65.77

Weight (kDa)

8.47

Isoelectric Point (pI)

44.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WHD_DRP PF23559 138 - 208 6.8e-13 Disease resistance protein Winged helix domain
LRR_14 PF23598 325 - 560 5.8e-29 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 418 - 519 2.7e-07 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1046
AccI GTMKAC 2 cut(s) 251, 1291
AciI CCGC 4 cut(s) 1110, 1282, 1363, 1433
AclWI GGATC 3 cut(s) 613, 626, 1071
AcoI YGGCCR 1 cut(s) 1631
AcsI RAATTY 6 cut(s) 321, 744, 1273, 1421, 1438, 1513
AcuI CTGAAG 3 cut(s) 848, 1083, 1332
AfaI GTAC 4 cut(s) 137, 1482, 1529, 1607
AfiI CCNNNNNNNGG 2 cut(s) 772, 1116
AgeI ACCGGT 1 cut(s) 1568
AjiI CACGTC 1 cut(s) 483
AjnI CCWGG 2 cut(s) 309, 1218
AjuI GAANNNNNNNTTGG 2 cut(s) 66, 98
AleI CACNNNNGTG 1 cut(s) 292
AluBI AGCT 7 cut(s) 34, 104, 653, 868, 1123, 1525, 1554
AluI AGCT 7 cut(s) 34, 104, 653, 868, 1123, 1525, 1554
Alw26I GTCTC 5 cut(s) 155, 323, 1082, 1495, 1604
AlwI GGATC 3 cut(s) 613, 626, 1071
Ama87I CYCGRG 1 cut(s) 1498
AoxI GGCC 5 cut(s) 141, 307, 443, 763, 1631
ApeKI GCWGC 1 cut(s) 1663
ApoI RAATTY 6 cut(s) 321, 744, 1273, 1421, 1438, 1513
AseI ATTAAT 1 cut(s) 555
AsiGI ACCGGT 1 cut(s) 1568
AspA2I CCTAGG 1 cut(s) 145
AspLEI GCGC 1 cut(s) 876
AspS9I GGNCC 6 cut(s) 142, 269, 307, 764, 1453, 1566
AsuC2I CCSGG 1 cut(s) 419
AsuHPI GGTGA 3 cut(s) 767, 1117, 1265
AvaI CYCGRG 1 cut(s) 1498
AvaII GGWCC 3 cut(s) 269, 1453, 1566
AvrII CCTAGG 1 cut(s) 145
BalI TGGCCA 1 cut(s) 1633
BamHI GGATCC 1 cut(s) 618
BauI CACGAG 1 cut(s) 1694
BbsI GAAGAC 3 cut(s) 189, 503, 924
BbvI GCAGC 1 cut(s) 1675
BccI CCATC 6 cut(s) 233, 416, 630, 672, 700, 755
BciT130I CCWGG 2 cut(s) 311, 1220
BciVI GTATCC 1 cut(s) 1475
BclI TGATCA 1 cut(s) 40
BcnI CCSGG 1 cut(s) 419
BcoDI GTCTC 5 cut(s) 155, 323, 1082, 1495, 1604
BfaI CTAG 3 cut(s) 146, 654, 1472
BfmI CTRYAG 3 cut(s) 252, 712, 1661
BfoI RGCGCY 1 cut(s) 877
BfuI GTATCC 1 cut(s) 1475
BglI GCCNNNNNGGC 1 cut(s) 1174
BglII AGATCT 1 cut(s) 367
BisI GCNGC 3 cut(s) 1283, 1363, 1664
BlnI CCTAGG 1 cut(s) 145
BlsI GCNGC 3 cut(s) 1284, 1364, 1665
BmcAI AGTACT 1 cut(s) 1607
Bme1390I CCNGG 3 cut(s) 311, 419, 1220
Bme18I GGWCC 3 cut(s) 269, 1453, 1566
BmeT110I CYCGRG 1 cut(s) 1498
BmgBI CACGTC 1 cut(s) 483
BmgT120I GGNCC 6 cut(s) 142, 269, 307, 764, 1453, 1566
BmiI GGNNCC 3 cut(s) 427, 620, 1567
BmrFI CCNGG 3 cut(s) 311, 419, 1220
BmrI ACTGGG 3 cut(s) 314, 1517, 1678
BmsI GCATC 2 cut(s) 584, 1083
BmuI ACTGGG 3 cut(s) 314, 1517, 1678
BpiI GAAGAC 3 cut(s) 189, 503, 924
BpmI CTGGAG 1 cut(s) 332
BpuEI CTTGAG 2 cut(s) 1073, 1586
BpuMI CCSGG 1 cut(s) 419
BsaI GGTCTC 1 cut(s) 155
BsaJI CCNNGG 6 cut(s) 145, 339, 614, 639, 1219, 1459
BsaWI WCCGGW 1 cut(s) 1568
Bsc4I CCNNNNNNNGG 2 cut(s) 772, 1116
Bse118I RCCGGY 1 cut(s) 1568
Bse1I ACTGG 5 cut(s) 309, 461, 568, 1512, 1673
BseBI CCWGG 2 cut(s) 311, 1220
BseDI CCNNGG 6 cut(s) 145, 339, 614, 639, 1219, 1459
BseGI GGATG 5 cut(s) 225, 427, 575, 683, 916
BseLI CCNNNNNNNGG 2 cut(s) 772, 1116
BseMII CTCAG 3 cut(s) 980, 1009, 1300
BseNI ACTGG 5 cut(s) 309, 461, 568, 1512, 1673
BseXI GCAGC 1 cut(s) 1675
BshFI GGCC 5 cut(s) 143, 309, 445, 765, 1633
BshTI ACCGGT 1 cut(s) 1568
BsiHKCI CYCGRG 1 cut(s) 1498
BsiSI CCGG 2 cut(s) 419, 1569
BslFI GGGAC 3 cut(s) 976, 1579, 1634
BslI CCNNNNNNNGG 2 cut(s) 772, 1116
BsmAI GTCTC 5 cut(s) 155, 323, 1082, 1495, 1604
BsmBI CGTCTC 2 cut(s) 1082, 1604
BsmFI GGGAC 3 cut(s) 976, 1579, 1634
BsmI GAATGC 1 cut(s) 693
BsnI GGCC 5 cut(s) 143, 309, 445, 765, 1633
Bso31I GGTCTC 1 cut(s) 155
BsoBI CYCGRG 1 cut(s) 1498
Bsp1407I TGTACA 2 cut(s) 135, 1527
Bsp19I CCATGG 1 cut(s) 339
BspACI CCGC 4 cut(s) 1110, 1282, 1363, 1433
BspANI GGCC 5 cut(s) 143, 309, 445, 765, 1633
BspCNI CTCAG 3 cut(s) 979, 1008, 1299
BspHI TCATGA 1 cut(s) 153
BspLI GGNNCC 3 cut(s) 427, 620, 1567
BspMAI CTGCAG 1 cut(s) 1665
BspPI GGATC 3 cut(s) 613, 626, 1071
BspTNI GGTCTC 1 cut(s) 155
BsrFI RCCGGY 1 cut(s) 1568
BsrGI TGTACA 2 cut(s) 135, 1527
BsrI ACTGG 5 cut(s) 309, 461, 568, 1512, 1673
BssAI RCCGGY 1 cut(s) 1568
BssECI CCNNGG 6 cut(s) 145, 339, 614, 639, 1219, 1459
BssNAI GTATAC 1 cut(s) 1292
BssSI CACGAG 1 cut(s) 1694
BssT1I CCWWGG 5 cut(s) 145, 339, 614, 639, 1459
Bst1107I GTATAC 1 cut(s) 1292
Bst2BI CACGAG 1 cut(s) 1694
Bst2UI CCWGG 2 cut(s) 311, 1220
Bst4CI ACNGT 2 cut(s) 256, 1691
Bst6I CTCTTC 3 cut(s) 434, 501, 1195
BstAPI GCANNNNNTGC 1 cut(s) 688
BstAUI TGTACA 2 cut(s) 135, 1527
BstC8I GCNNGC 4 cut(s) 720, 724, 878, 1166
BstDEI CTNAG 7 cut(s) 727, 966, 989, 995, 1084, 1286, 1555
BstDSI CCRYGG 1 cut(s) 339
BstF5I GGATG 5 cut(s) 225, 427, 575, 683, 916
BstH2I RGCGCY 1 cut(s) 877
BstHHI GCGC 1 cut(s) 876
BstMAI GTCTC 5 cut(s) 155, 323, 1082, 1495, 1604
BstMWI GCNNNNNNNGC 3 cut(s) 650, 688, 1174
BstNI CCWGG 2 cut(s) 311, 1220
BstNSI RCATGY 3 cut(s) 726, 1077, 1168
BstSCI CCNGG 3 cut(s) 309, 417, 1218
BstSFI CTRYAG 3 cut(s) 252, 712, 1661
BstV1I GCAGC 1 cut(s) 1675
BstV2I GAAGAC 3 cut(s) 189, 503, 924
BstX2I RGATCY 3 cut(s) 367, 618, 1063
BstYI RGATCY 3 cut(s) 367, 618, 1063
BstZ17I GTATAC 1 cut(s) 1292
BsuI GTATCC 1 cut(s) 1475
BsuRI GGCC 5 cut(s) 143, 309, 445, 765, 1633
BtgI CCRYGG 1 cut(s) 339
BtrI CACGTC 1 cut(s) 483
BtsCI GGATG 5 cut(s) 225, 427, 575, 683, 916
BtsIMutI CAGTG 2 cut(s) 1666, 1687
Cac8I GCNNGC 4 cut(s) 720, 724, 878, 1166
CciI TCATGA 1 cut(s) 153
CfoI GCGC 1 cut(s) 876
Cfr10I RCCGGY 1 cut(s) 1568
Cfr13I GGNCC 6 cut(s) 142, 269, 307, 764, 1453, 1566
CseI GACGC 1 cut(s) 1586
Csp6I GTAC 4 cut(s) 136, 1481, 1528, 1606
CspAI ACCGGT 1 cut(s) 1568
CviAII CATG 9 cut(s) 154, 218, 340, 379, 723, 1074, 1165, 1297, 1562
CviQI GTAC 4 cut(s) 136, 1481, 1528, 1606
DdeI CTNAG 7 cut(s) 727, 966, 989, 995, 1084, 1286, 1555
DraI TTTAAA 2 cut(s) 1278, 1518
EaeI YGGCCR 1 cut(s) 1631
Eam1104I CTCTTC 3 cut(s) 434, 501, 1195
EarI CTCTTC 3 cut(s) 434, 501, 1195
EciI GGCGGA 1 cut(s) 1422
Eco130I CCWWGG 5 cut(s) 145, 339, 614, 639, 1459
Eco147I AGGCCT 1 cut(s) 445
Eco31I GGTCTC 1 cut(s) 155
Eco32I GATATC 1 cut(s) 1105
Eco47I GGWCC 3 cut(s) 269, 1453, 1566
Eco57I CTGAAG 3 cut(s) 848, 1083, 1332
Eco88I CYCGRG 1 cut(s) 1498
EcoO109I RGGNCCY 1 cut(s) 142
EcoRI GAATTC 1 cut(s) 1421
EcoRII CCWGG 2 cut(s) 309, 1218
EcoRV GATATC 1 cut(s) 1105
EcoT14I CCWWGG 5 cut(s) 145, 339, 614, 639, 1459
EcoT22I ATGCAT 1 cut(s) 577
ErhI CCWWGG 5 cut(s) 145, 339, 614, 639, 1459
Esp3I CGTCTC 2 cut(s) 1082, 1604
FaeI CATG 9 cut(s) 157, 221, 343, 382, 726, 1077, 1168, 1300, 1565
FalI AAGNNNNNCTT 6 cut(s) 80, 112, 343, 375, 380, 412
FaqI GGGAC 3 cut(s) 976, 1579, 1634
FatI CATG 9 cut(s) 153, 217, 339, 378, 722, 1073, 1164, 1296, 1561
FbaI TGATCA 1 cut(s) 40
FblI GTMKAC 2 cut(s) 251, 1291
Fnu4HI GCNGC 3 cut(s) 1283, 1363, 1664
FokI GGATG 5 cut(s) 212, 434, 562, 690, 923
Fsp4HI GCNGC 3 cut(s) 1283, 1363, 1664
FspBI CTAG 3 cut(s) 146, 654, 1472
GlaI GCGC 1 cut(s) 875
GluI GCNGC 3 cut(s) 1283, 1363, 1664
GsuI CTGGAG 1 cut(s) 332
HaeII RGCGCY 1 cut(s) 877
HaeIII GGCC 5 cut(s) 143, 309, 445, 765, 1633
HapII CCGG 2 cut(s) 419, 1569
HgaI GACGC 1 cut(s) 1586
HhaI GCGC 1 cut(s) 876
Hin1II CATG 9 cut(s) 157, 221, 343, 382, 726, 1077, 1168, 1300, 1565
Hin6I GCGC 1 cut(s) 874
HinP1I GCGC 1 cut(s) 874
HincII GTYRAC 1 cut(s) 486
HindII GTYRAC 1 cut(s) 486
HinfI GANTC 8 cut(s) 185, 233, 331, 671, 884, 964, 1057, 1307
HpaII CCGG 2 cut(s) 419, 1569
HphI GGTGA 3 cut(s) 767, 1117, 1265
Hpy166II GTNNAC 7 cut(s) 63, 252, 486, 775, 928, 1247, 1292
Hpy188I TCNGA 9 cut(s) 174, 279, 450, 969, 998, 1102, 1144, 1206, 1312
Hpy188III TCNNGA 7 cut(s) 154, 267, 371, 675, 1008, 1061, 1694
Hpy8I GTNNAC 7 cut(s) 63, 252, 486, 775, 928, 1247, 1292
HpyAV CCTTC 6 cut(s) 844, 1035, 1107, 1336, 1576, 1636
HpyCH4III ACNGT 2 cut(s) 256, 1691
HpyCH4IV ACGT 3 cut(s) 482, 1479, 1710
HpyCH4V TGCA 6 cut(s) 221, 244, 575, 722, 808, 1663
HpyF10VI GCNNNNNNNGC 3 cut(s) 650, 688, 1174
HpyF3I CTNAG 7 cut(s) 727, 966, 989, 995, 1084, 1286, 1555
HpySE526I ACGT 3 cut(s) 482, 1479, 1710
Hsp92II CATG 9 cut(s) 157, 221, 343, 382, 726, 1077, 1168, 1300, 1565
HspAI GCGC 1 cut(s) 874
Ksp22I TGATCA 1 cut(s) 40
LmnI GCTCC 2 cut(s) 101, 1200
Lsp1109I GCAGC 1 cut(s) 1675
LweI GCATC 2 cut(s) 584, 1083
MaeI CTAG 3 cut(s) 146, 654, 1472
MaeII ACGT 3 cut(s) 482, 1479, 1710
MaeIII GTNAC 5 cut(s) 234, 285, 297, 1076, 1691
MflI RGATCY 3 cut(s) 367, 618, 1063
MlsI TGGCCA 1 cut(s) 1633
MluNI TGGCCA 1 cut(s) 1633
MlyI GAGTC 6 cut(s) 179, 242, 325, 878, 958, 1316
MmeI TCCRAC 1 cut(s) 1122
Mox20I TGGCCA 1 cut(s) 1633
Mph1103I ATGCAT 1 cut(s) 577
MscI TGGCCA 1 cut(s) 1633
MslI CAYNNNNRTG 1 cut(s) 292
Msp20I TGGCCA 1 cut(s) 1633
MspI CCGG 2 cut(s) 419, 1569
MspR9I CCNGG 3 cut(s) 311, 419, 1220
Mva1269I GAATGC 1 cut(s) 693
MvaI CCWGG 2 cut(s) 311, 1220
MwoI GCNNNNNNNGC 3 cut(s) 650, 688, 1174
NciI CCSGG 1 cut(s) 419
NcoI CCATGG 1 cut(s) 339
NlaIII CATG 9 cut(s) 157, 221, 343, 382, 726, 1077, 1168, 1300, 1565
NlaIV GGNNCC 3 cut(s) 427, 620, 1567
NmeAIII GCCGAG 1 cut(s) 866
NmuCI GTSAC 5 cut(s) 234, 285, 297, 1076, 1691
NsiI ATGCAT 1 cut(s) 577
NspI RCATGY 3 cut(s) 726, 1077, 1168
OliI CACNNNNGTG 1 cut(s) 292
PaeI GCATGC 2 cut(s) 726, 1168
PagI TCATGA 1 cut(s) 153
PceI AGGCCT 1 cut(s) 445
PctI GAATGC 1 cut(s) 693
PfeI GAWTC 2 cut(s) 671, 1057
PfoI TCCNGGA 1 cut(s) 417
PinAI ACCGGT 1 cut(s) 1568
PkrI GCNGC 3 cut(s) 1284, 1364, 1665
PleI GAGTC 6 cut(s) 179, 241, 325, 878, 958, 1315
PpsI GAGTC 6 cut(s) 179, 241, 325, 878, 958, 1315
PshBI ATTAAT 1 cut(s) 555
PsiI TTATAA 1 cut(s) 1046
Psp6I CCWGG 2 cut(s) 309, 1218
PspGI CCWGG 2 cut(s) 309, 1218
PspN4I GGNNCC 3 cut(s) 427, 620, 1567
PspPI GGNCC 6 cut(s) 142, 269, 307, 764, 1453, 1566
PstI CTGCAG 1 cut(s) 1665
PsuI RGATCY 3 cut(s) 367, 618, 1063
RsaI GTAC 4 cut(s) 137, 1482, 1529, 1607
RsaNI GTAC 4 cut(s) 136, 1481, 1528, 1606
RseI CAYNNNNRTG 1 cut(s) 292
SatI GCNGC 3 cut(s) 1283, 1363, 1664
Sau96I GGNCC 6 cut(s) 142, 269, 307, 764, 1453, 1566
ScaI AGTACT 1 cut(s) 1607
SchI GAGTC 6 cut(s) 179, 242, 325, 878, 958, 1316
ScrFI CCNGG 3 cut(s) 311, 419, 1220
SfaNI GCATC 2 cut(s) 584, 1083
SfcI CTRYAG 3 cut(s) 252, 712, 1661
SinI GGWCC 3 cut(s) 269, 1453, 1566
SmiMI CAYNNNNRTG 1 cut(s) 292
SmlI CTYRAG 2 cut(s) 1052, 1601
SmoI CTYRAG 2 cut(s) 1052, 1601
SphI GCATGC 2 cut(s) 726, 1168
SseBI AGGCCT 1 cut(s) 445
SsiI CCGC 4 cut(s) 1110, 1282, 1363, 1433
SspMI CTAG 3 cut(s) 146, 654, 1472
StuI AGGCCT 1 cut(s) 445
StyD4I CCNGG 3 cut(s) 309, 417, 1218
StyI CCWWGG 5 cut(s) 145, 339, 614, 639, 1459
TaaI ACNGT 2 cut(s) 256, 1691
TaiI ACGT 3 cut(s) 485, 1482, 1713
TaqI TCGA 4 cut(s) 53, 168, 258, 1069
TatI WGTACW 3 cut(s) 135, 1527, 1605
TauI GCSGC 2 cut(s) 1285, 1365
TfiI GAWTC 2 cut(s) 671, 1057
TscAI CASTG 2 cut(s) 1673, 1694
TseFI GTSAC 5 cut(s) 234, 285, 297, 1076, 1691
TseI GCWGC 1 cut(s) 1663
Tsp45I GTSAC 5 cut(s) 234, 285, 297, 1076, 1691
TspDTI ATGAA 7 cut(s) 170, 378, 449, 466, 930, 1341, 1434
TspGWI ACGGA 3 cut(s) 418, 576, 1384
TspRI CASTG 2 cut(s) 1673, 1694
VpaK11BI GGWCC 3 cut(s) 269, 1453, 1566
VspI ATTAAT 1 cut(s) 555
XapI RAATTY 6 cut(s) 321, 744, 1273, 1421, 1438, 1513
XceI RCATGY 3 cut(s) 726, 1077, 1168
XmaJI CCTAGG 1 cut(s) 145
XmiI GTMKAC 2 cut(s) 251, 1291
XspI CTAG 3 cut(s) 146, 654, 1472
ZrmI AGTACT 1 cut(s) 1607
Zsp2I ATGCAT 1 cut(s) 577
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.