Rh2BG505400

Encoded by

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0000917
Physical Location & Seq
Forward (+)
71078394 .. 71080401
2008 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG505400.1

Sequence Viewer

Length: 1833 bp
ATGCCCAAGTTGCTGGATCATCTGAGAAAAGCCAGAGCCGTGGCCGACACAGATGACGAAGCTTTACTCGTGAGCTTCAAAAAGATGGAAGATGAGCTTGAGAACATCAAAGGCGTGCTACCCAGAGTGAAGCACTGGGAGCAAACTCTGGTGAACCAGTTCACTAACTTGGAGCGCCGCCTCGACAAGATCATCTTGGGCACTGAGACTTCCATCGAGGCCATCACCGCCACACTAAACCGCATGTTTCAAAATGTGGAGCAAATCAAACTCTCACATCCTCTAGTGCAGCTGATGCCTTCACCCGAGCGCAGCTCCGAGTCGACATGGACGCGCAGATCTCAGCCGGAGTTGAGTCAGGGAATGTCACCCGAATGGTCCGAATTGAGCCTCGAAAATTGGGTTGATAACACTCGGAGCATGGAGGATATAAAGAGAAGCTATGAACATCTTGAGAGCGTTGAGTTGAAGATGTGCTGTCTATCTCTTTCAATATTCCCTCCTGATTCTGTCATAAAGAAGAGGCCTCTGATTTACTGGTGGATTGGCGAGGGTTTCGTTACAACCACTAAAGGCAGGACAGCTGAAGAGACAGGTGAGGAAATCTTCCGGAAACTCATAATCTCGGGTTTGATTCAAGTTCAACCACATAATGCATTTAGTTCTATAGTTGTGAATAGTTGTACAGTGCATCCTTGGGTTCGTCATATGTTGGTCTCCTTGGCCAAGCACGCTAAGCTTTTCGACTTCGATTCAAGATGGCCAAGGATGCCGTCTTATAGTACAGATAGTATTTCAAATTGTAGACGTGCATGTTTAGTTTATGACAACCCAATTTCTGAGGGGTCTAATGGCCGGAAAGTGGAGGATCTGTTGACAGTGTTCAATGTGAACGAACAGTATCTTAGTTTGAAACGGGAATGGCTTAGGAAGTTGAAAAAAGTTGAGGTGATTCAGCTAGGACGGTGGCAGAACTCGGCTTCGCATCACATTGAAGTCGAAGATAAAGGGTTTTTGAAAGGTTTAGGGGCTCAGAATAAGCTCTTGAAGTATCTTTGCCTCCGAGGGATATCAGGAATTACCAAACTCCCTTCTTCTATCTTGAAGCTTCTCAACCTTGAAATTCTTGATCTGAGGGCATGCCACAGCTTAGAGACTTTGCCTCCAGATATCTCACCATTGAGGAATCTCACGCATTTCGACATATCCGAGTGTTACTTGCTAGAAGCAATGCCGAAAGGAATTGAGAAGCTGTCTTCTCTTCAAGTGCTAAAAGGCTTTGTGATTGGGGATTTGGAAGGAACTCCCTGCAGAATTGGTGATCTTGCACAGCTGAGGCATCTAAAGCGGCTCAGTATACACATGGGCAATCAGGCTGAAGTTCAAAAGGGGGAGTTCCACAAATTGGAGGAAATTGTATCTCTTCGCCACCTCAAACTTTCATGGGGAGTTGTTTCTCCAAACTTGAAAGCAAAAGTTGCCTTCCAGTCCTTGGAGTTCTCTTTTCCGCCGGATTTAGAAAAATTGGATCTTCAAGGCATCCCTCTAGCACATGTACCAGAATGGTTGAACCCTGTCAAGCTCAAGAATTTAAAAAAGCTCTTTATAAGGGGTGGGGAACTAGATAGCTTGGATCATGGAGAGACTGAGAAGTGGACTGTTGAAGTCTTGCGTCTCAAGCACTTGAATAATTTGGAGATAGGTTTGCCAAAGTTGAAGGATGAGTTTCCTCGTCTGCGCTATTTGGAGAAAATCAACTGTCATGAGATTGAAAAGGATCGATACGATGAAGACATTGTTTGGAGTGAATATGCAGAGTACACCTGCATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

610

Amino Acids

70.24

Weight (kDa)

6.87

Isoelectric Point (pI)

49.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WHD_DRP PF23559 165 - 235 3.1e-13 Disease resistance protein Winged helix domain
LRR_14 PF23598 348 - 541 2.2e-24 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1607
AccB7I CCANNNNNTGG 2 cut(s) 1405, 1492
AccI GTMKAC 3 cut(s) 323, 805, 1357
AccII CGCG 1 cut(s) 334
AccIII TCCGGA 1 cut(s) 609
AciI CCGC 5 cut(s) 178, 228, 241, 1348, 1508
AclWI GGATC 5 cut(s) 24, 876, 1536, 1641, 1785
AcoI YGGCCR 4 cut(s) 42, 723, 761, 853
AcsI RAATTY 2 cut(s) 1122, 1588
AcuI CTGAAG 2 cut(s) 606, 1398
AfaI GTAC 4 cut(s) 685, 784, 1557, 1820
AfiI CCNNNNNNNGG 3 cut(s) 862, 1405, 1492
AflIII ACRYGT 1 cut(s) 1552
AjiI CACGTC 1 cut(s) 809
Alw26I GTCTC 6 cut(s) 200, 584, 721, 1148, 1637, 1679
AlwI GGATC 5 cut(s) 24, 876, 1536, 1641, 1785
Ama87I CYCGRG 2 cut(s) 305, 625
Aor13HI TCCGGA 1 cut(s) 609
AoxI GGCC 6 cut(s) 42, 219, 524, 723, 761, 853
ApeKI GCWGC 2 cut(s) 289, 312
ApoI RAATTY 2 cut(s) 1122, 1588
Asp700I GAANNNNTTC 1 cut(s) 158
AspLEI GCGC 4 cut(s) 177, 312, 336, 1740
AspS9I GGNCC 1 cut(s) 378
AsuHPI GGTGA 8 cut(s) 163, 217, 294, 360, 608, 961, 1167, 1331
AvaI CYCGRG 2 cut(s) 305, 625
AvaII GGWCC 1 cut(s) 378
BaeGI GKGCMC 1 cut(s) 203
BalI TGGCCA 2 cut(s) 725, 763
BanII GRGCYC 1 cut(s) 1033
BauI CACGAG 1 cut(s) 68
BbsI GAAGAC 2 cut(s) 1248, 1797
BbvCI CCTCAGC 1 cut(s) 1334
BbvI GCAGC 2 cut(s) 301, 324
BccI CCATC 4 cut(s) 79, 221, 230, 753
BceAI ACGGC 2 cut(s) 23, 757
BcoDI GTCTC 6 cut(s) 200, 584, 721, 1148, 1637, 1679
BfaI CTAG 5 cut(s) 284, 959, 1223, 1547, 1622
BfmI CTRYAG 2 cut(s) 666, 1309
BfoI RGCGCY 1 cut(s) 178
BglII AGATCT 1 cut(s) 338
BisI GCNGC 4 cut(s) 178, 290, 313, 1349
BlpI GCTNAGC 1 cut(s) 735
BlsI GCNGC 4 cut(s) 179, 291, 314, 1350
Bme18I GGWCC 1 cut(s) 378
BmeT110I CYCGRG 2 cut(s) 305, 625
BmgBI CACGTC 1 cut(s) 809
BmgT120I GGNCC 1 cut(s) 378
BmrI ACTGGG 1 cut(s) 145
BmsI GCATC 6 cut(s) 285, 700, 759, 994, 1348, 1548
BmuI ACTGGG 1 cut(s) 145
BpiI GAAGAC 2 cut(s) 1248, 1797
BplI GAGNNNNNCTC 2 cut(s) 299, 331
BpmI CTGGAG 1 cut(s) 1149
Bpu10I CCTNAGC 2 cut(s) 926, 1334
Bpu1102I GCTNAGC 1 cut(s) 735
BpuEI CTTGAG 4 cut(s) 119, 473, 1568, 1661
Bsa29I ATCGAT 1 cut(s) 1780
BsaI GGTCTC 1 cut(s) 721
BsaJI CCNNGG 6 cut(s) 39, 695, 720, 764, 1063, 1491
BsaWI WCCGGW 1 cut(s) 609
BsaXI ACNNNNNCTCC 4 cut(s) 857, 887, 1147, 1177
Bsc4I CCNNNNNNNGG 3 cut(s) 862, 1405, 1492
Bse1I ACTGG 4 cut(s) 140, 157, 542, 1486
Bse3DI GCAATG 1 cut(s) 1236
BseAI TCCGGA 1 cut(s) 609
BseCI ATCGAT 1 cut(s) 1780
BseDI CCNNGG 6 cut(s) 39, 695, 720, 764, 1063, 1491
BseGI GGATG 5 cut(s) 277, 691, 774, 1539, 1726
BseLI CCNNNNNNNGG 3 cut(s) 862, 1405, 1492
BseMI GCAATG 1 cut(s) 1236
BseMII CTCAG 9 cut(s) 14, 195, 356, 831, 1046, 1124, 1325, 1366, 1638
BseNI ACTGG 4 cut(s) 140, 157, 542, 1486
BseSI GKGCMC 1 cut(s) 203
BseXI GCAGC 2 cut(s) 301, 324
BsgI GTGCAG 1 cut(s) 308
Bsh1236I CGCG 1 cut(s) 334
BshFI GGCC 6 cut(s) 44, 221, 526, 725, 763, 855
BshVI ATCGAT 1 cut(s) 1780
BsiHKCI CYCGRG 2 cut(s) 305, 625
BsiSI CCGG 4 cut(s) 347, 610, 856, 1511
BslI CCNNNNNNNGG 3 cut(s) 862, 1405, 1492
BsmAI GTCTC 6 cut(s) 200, 584, 721, 1148, 1637, 1679
BsmBI CGTCTC 1 cut(s) 1679
BsnI GGCC 6 cut(s) 44, 221, 526, 725, 763, 855
Bso31I GGTCTC 1 cut(s) 721
BsoBI CYCGRG 2 cut(s) 305, 625
Bsp1286I GDGCHC 2 cut(s) 203, 1033
Bsp13I TCCGGA 1 cut(s) 609
Bsp1407I TGTACA 1 cut(s) 683
Bsp143I GATC 9 cut(s) 16, 189, 338, 868, 1129, 1321, 1528, 1633, 1777
Bsp1720I GCTNAGC 1 cut(s) 735
BspACI CCGC 5 cut(s) 178, 228, 241, 1348, 1508
BspANI GGCC 6 cut(s) 44, 221, 526, 725, 763, 855
BspCNI CTCAG 9 cut(s) 15, 196, 355, 832, 1045, 1125, 1326, 1365, 1639
BspDI ATCGAT 1 cut(s) 1780
BspEI TCCGGA 1 cut(s) 609
BspFNI CGCG 1 cut(s) 334
BspHI TCATGA 1 cut(s) 1762
BspMAI CTGCAG 1 cut(s) 1313
BspPI GGATC 5 cut(s) 24, 876, 1536, 1641, 1785
BspTNI GGTCTC 1 cut(s) 721
BsrDI GCAATG 1 cut(s) 1236
BsrGI TGTACA 1 cut(s) 683
BsrI ACTGG 4 cut(s) 140, 157, 542, 1486
BssECI CCNNGG 6 cut(s) 39, 695, 720, 764, 1063, 1491
BssMI GATC 9 cut(s) 16, 189, 338, 868, 1129, 1321, 1528, 1633, 1777
BssNAI GTATAC 1 cut(s) 1358
BssSI CACGAG 1 cut(s) 68
BssT1I CCWWGG 4 cut(s) 695, 720, 764, 1491
Bst1107I GTATAC 1 cut(s) 1358
Bst2BI CACGAG 1 cut(s) 68
Bst4CI ACNGT 6 cut(s) 688, 880, 900, 966, 1660, 1760
Bst6I CTCTTC 4 cut(s) 515, 582, 1266, 1428
BstAPI GCANNNNNTGC 2 cut(s) 295, 1478
BstAUI TGTACA 1 cut(s) 683
BstC8I GCNNGC 3 cut(s) 116, 732, 1141
BstDSI CCRYGG 1 cut(s) 39
BstF5I GGATG 5 cut(s) 277, 691, 774, 1539, 1726
BstFNI CGCG 1 cut(s) 334
BstH2I RGCGCY 1 cut(s) 178
BstHHI GCGC 4 cut(s) 177, 312, 336, 1740
BstKTI GATC 9 cut(s) 19, 192, 341, 871, 1132, 1324, 1531, 1636, 1780
BstMAI GTCTC 6 cut(s) 200, 584, 721, 1148, 1637, 1679
BstMBI GATC 9 cut(s) 16, 189, 338, 868, 1129, 1321, 1528, 1633, 1777
BstNSI RCATGY 4 cut(s) 247, 816, 1143, 1556
BstSFI CTRYAG 2 cut(s) 666, 1309
BstSLI GKGCMC 1 cut(s) 203
BstUI CGCG 1 cut(s) 334
BstV1I GCAGC 2 cut(s) 301, 324
BstV2I GAAGAC 2 cut(s) 1248, 1797
BstX2I RGATCY 3 cut(s) 338, 868, 1528
BstXI CCANNNNNNTGG 2 cut(s) 13, 40
BstYI RGATCY 3 cut(s) 338, 868, 1528
BstZ17I GTATAC 1 cut(s) 1358
Bsu15I ATCGAT 1 cut(s) 1780
BsuRI GGCC 6 cut(s) 44, 221, 526, 725, 763, 855
BsuTUI ATCGAT 1 cut(s) 1780
BtgI CCRYGG 1 cut(s) 39
BtrI CACGTC 1 cut(s) 809
BtsCI GGATG 5 cut(s) 277, 691, 774, 1539, 1726
BtsIMutI CAGTG 4 cut(s) 133, 201, 693, 885
Cac8I GCNNGC 3 cut(s) 116, 732, 1141
CciI TCATGA 1 cut(s) 1762
CfoI GCGC 4 cut(s) 177, 312, 336, 1740
Cfr13I GGNCC 1 cut(s) 378
ClaI ATCGAT 1 cut(s) 1780
CseI GACGC 2 cut(s) 340, 1661
Csp6I GTAC 4 cut(s) 684, 783, 1556, 1819
CviQI GTAC 4 cut(s) 684, 783, 1556, 1819
DpnI GATC 9 cut(s) 18, 191, 340, 870, 1131, 1323, 1530, 1635, 1779
DpnII GATC 9 cut(s) 16, 189, 338, 868, 1129, 1321, 1528, 1633, 1777
DraI TTTAAA 1 cut(s) 1593
EaeI YGGCCR 4 cut(s) 42, 723, 761, 853
Eam1104I CTCTTC 4 cut(s) 515, 582, 1266, 1428
EarI CTCTTC 4 cut(s) 515, 582, 1266, 1428
EciI GGCGGA 1 cut(s) 1497
Eco130I CCWWGG 4 cut(s) 695, 720, 764, 1491
Eco147I AGGCCT 1 cut(s) 526
Eco24I GRGCYC 1 cut(s) 1033
Eco31I GGTCTC 1 cut(s) 721
Eco32I GATATC 2 cut(s) 1071, 1171
Eco47I GGWCC 1 cut(s) 378
Eco57I CTGAAG 2 cut(s) 606, 1398
Eco88I CYCGRG 2 cut(s) 305, 625
EcoRV GATATC 2 cut(s) 1071, 1171
EcoT14I CCWWGG 4 cut(s) 695, 720, 764, 1491
EcoT22I ATGCAT 1 cut(s) 658
EcoT38I GRGCYC 1 cut(s) 1033
ErhI CCWWGG 4 cut(s) 695, 720, 764, 1491
Esp3I CGTCTC 1 cut(s) 1679
FalI AAGNNNNNCTT 4 cut(s) 81, 113, 179, 211
FauNDI CATATG 2 cut(s) 708, 1829
FblI GTMKAC 3 cut(s) 323, 805, 1357
Fnu4HI GCNGC 4 cut(s) 178, 290, 313, 1349
FokI GGATG 5 cut(s) 264, 678, 781, 1526, 1733
FriOI GRGCYC 1 cut(s) 1033
Fsp4HI GCNGC 4 cut(s) 178, 290, 313, 1349
FspBI CTAG 5 cut(s) 284, 959, 1223, 1547, 1622
GlaI GCGC 4 cut(s) 176, 311, 335, 1739
GluI GCNGC 4 cut(s) 178, 290, 313, 1349
GsuI CTGGAG 1 cut(s) 1149
HaeII RGCGCY 1 cut(s) 178
HaeIII GGCC 6 cut(s) 44, 221, 526, 725, 763, 855
HapII CCGG 4 cut(s) 347, 610, 856, 1511
HgaI GACGC 2 cut(s) 340, 1661
HhaI GCGC 4 cut(s) 177, 312, 336, 1740
Hin6I GCGC 4 cut(s) 175, 310, 334, 1738
HinP1I GCGC 4 cut(s) 175, 310, 334, 1738
HincII GTYRAC 2 cut(s) 324, 876
HindII GTYRAC 2 cut(s) 324, 876
HindIII AAGCTT 3 cut(s) 60, 737, 1106
HinfI GANTC 7 cut(s) 320, 355, 506, 634, 752, 952, 1186
HpaII CCGG 4 cut(s) 347, 610, 856, 1511
HphI GGTGA 8 cut(s) 163, 217, 294, 360, 608, 961, 1167, 1331
Hpy166II GTNNAC 9 cut(s) 154, 162, 324, 806, 876, 892, 1358, 1656, 1821
Hpy8I GTNNAC 9 cut(s) 154, 162, 324, 806, 876, 892, 1358, 1656, 1821
HpyAV CCTTC 5 cut(s) 309, 1101, 1292, 1492, 1711
HpyCH4III ACNGT 6 cut(s) 688, 880, 900, 966, 1660, 1760
HpyCH4IV ACGT 1 cut(s) 808
HpyCH4V TGCA 8 cut(s) 289, 656, 691, 812, 1311, 1328, 1814, 1827
HpySE526I ACGT 1 cut(s) 808
HspAI GCGC 4 cut(s) 175, 310, 334, 1738
Kpn2I TCCGGA 1 cut(s) 609
Kzo9I GATC 9 cut(s) 16, 189, 338, 868, 1129, 1321, 1528, 1633, 1777
LmnI GCTCC 5 cut(s) 139, 172, 259, 320, 417
Lsp1109I GCAGC 2 cut(s) 301, 324
LweI GCATC 6 cut(s) 285, 700, 759, 994, 1348, 1548
MaeI CTAG 5 cut(s) 284, 959, 1223, 1547, 1622
MaeII ACGT 1 cut(s) 808
MaeIII GTNAC 3 cut(s) 366, 559, 1214
MalI GATC 9 cut(s) 18, 191, 340, 870, 1131, 1323, 1530, 1635, 1779
MboI GATC 9 cut(s) 16, 189, 338, 868, 1129, 1321, 1528, 1633, 1777
MflI RGATCY 3 cut(s) 338, 868, 1528
MhlI GDGCHC 2 cut(s) 203, 1033
MlsI TGGCCA 2 cut(s) 725, 763
MluNI TGGCCA 2 cut(s) 725, 763
MlyI GAGTC 2 cut(s) 329, 364
Mox20I TGGCCA 2 cut(s) 725, 763
Mph1103I ATGCAT 1 cut(s) 658
MroI TCCGGA 1 cut(s) 609
MroXI GAANNNNTTC 1 cut(s) 158
MscI TGGCCA 2 cut(s) 725, 763
MseI TTAA 1 cut(s) 1592
MslI CAYNNNNRTG 1 cut(s) 373
Msp20I TGGCCA 2 cut(s) 725, 763
MspA1I CMGCKG 3 cut(s) 292, 584, 1333
MspI CCGG 4 cut(s) 347, 610, 856, 1511
MvnI CGCG 1 cut(s) 334
NdeI CATATG 2 cut(s) 708, 1829
NdeII GATC 9 cut(s) 16, 189, 338, 868, 1129, 1321, 1528, 1633, 1777
NmeAIII GCCGAG 1 cut(s) 956
NmuCI GTSAC 1 cut(s) 366
NsiI ATGCAT 1 cut(s) 658
NspI RCATGY 4 cut(s) 247, 816, 1143, 1556
PaeI GCATGC 1 cut(s) 1143
PagI TCATGA 1 cut(s) 1762
PceI AGGCCT 1 cut(s) 526
PciI ACATGT 1 cut(s) 1552
PcsI WCGNNNNNNNCGW 1 cut(s) 1206
PdmI GAANNNNTTC 1 cut(s) 158
PfeI GAWTC 5 cut(s) 506, 634, 752, 952, 1186
PflMI CCANNNNNTGG 2 cut(s) 1405, 1492
PkrI GCNGC 4 cut(s) 179, 291, 314, 1350
PleI GAGTC 2 cut(s) 328, 363
PpsI GAGTC 2 cut(s) 328, 363
PscI ACATGT 1 cut(s) 1552
PsiI TTATAA 1 cut(s) 1607
PspPI GGNCC 1 cut(s) 378
PstI CTGCAG 1 cut(s) 1313
PsuI RGATCY 3 cut(s) 338, 868, 1528
PvuII CAGCTG 3 cut(s) 292, 584, 1333
RsaI GTAC 4 cut(s) 685, 784, 1557, 1820
RsaNI GTAC 4 cut(s) 684, 783, 1556, 1819
RseI CAYNNNNRTG 1 cut(s) 373
SalI GTCGAC 1 cut(s) 322
SaqAI TTAA 1 cut(s) 1592
SatI GCNGC 4 cut(s) 178, 290, 313, 1349
Sau3AI GATC 9 cut(s) 16, 189, 338, 868, 1129, 1321, 1528, 1633, 1777
Sau96I GGNCC 1 cut(s) 378
SchI GAGTC 2 cut(s) 329, 364
SduI GDGCHC 2 cut(s) 203, 1033
SfaNI GCATC 6 cut(s) 285, 700, 759, 994, 1348, 1548
SfcI CTRYAG 2 cut(s) 666, 1309
SinI GGWCC 1 cut(s) 378
SmiMI CAYNNNNRTG 1 cut(s) 373
SmlI CTYRAG 4 cut(s) 98, 452, 1583, 1676
SmoI CTYRAG 4 cut(s) 98, 452, 1583, 1676
SphI GCATGC 1 cut(s) 1143
SseBI AGGCCT 1 cut(s) 526
SsiI CCGC 5 cut(s) 178, 228, 241, 1348, 1508
SspI AATATT 1 cut(s) 495
SspMI CTAG 5 cut(s) 284, 959, 1223, 1547, 1622
StuI AGGCCT 1 cut(s) 526
StyI CCWWGG 4 cut(s) 695, 720, 764, 1491
TaaI ACNGT 6 cut(s) 688, 880, 900, 966, 1660, 1760
TaiI ACGT 1 cut(s) 811
TaqI TCGA 9 cut(s) 183, 216, 323, 393, 744, 750, 999, 1200, 1780
TatI WGTACW 3 cut(s) 683, 782, 1818
TauI GCSGC 2 cut(s) 180, 1351
TfiI GAWTC 5 cut(s) 506, 634, 752, 952, 1186
Tru1I TTAA 1 cut(s) 1592
Tru9I TTAA 1 cut(s) 1592
TscAI CASTG 4 cut(s) 140, 208, 693, 885
TseFI GTSAC 1 cut(s) 366
TseI GCWGC 2 cut(s) 289, 312
Tsp45I GTSAC 1 cut(s) 366
TspDTI ATGAA 3 cut(s) 459, 1431, 1803
TspRI CASTG 4 cut(s) 140, 208, 693, 885
Van91I CCANNNNNTGG 2 cut(s) 1405, 1492
VpaK11BI GGWCC 1 cut(s) 378
XapI RAATTY 2 cut(s) 1122, 1588
XceI RCATGY 4 cut(s) 247, 816, 1143, 1556
XmiI GTMKAC 3 cut(s) 323, 805, 1357
XmnI GAANNNNTTC 1 cut(s) 158
XspI CTAG 5 cut(s) 284, 959, 1223, 1547, 1622
Zsp2I ATGCAT 1 cut(s) 658
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.