Encoded by
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 1610 |
| AatII | GACGTC | 1 cut(s) | 114 |
| Acc36I | ACCTGC | 1 cut(s) | 1610 |
| Acc65I | GGTACC | 1 cut(s) | 154 |
| AccB1I | GGYRCC | 3 cut(s) | 154, 1368, 1449 |
| AccI | GTMKAC | 3 cut(s) | 5, 909, 1378 |
| AccII | CGCG | 1 cut(s) | 213 |
| AciI | CCGC | 8 cut(s) | 66, 213, 234, 305, 327, 395, 758, 1838 |
| AclWI | GGATC | 1 cut(s) | 1158 |
| AcoI | YGGCCR | 2 cut(s) | 124, 794 |
| AcsI | RAATTY | 5 cut(s) | 26, 720, 1143, 1458, 1597 |
| AcuI | CTGAAG | 5 cut(s) | 354, 903, 974, 1206, 1878 |
| AcyI | GRCGYC | 3 cut(s) | 111, 803, 1369 |
| AfaI | GTAC | 5 cut(s) | 156, 364, 1290, 1324, 1566 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 615, 874 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 272 |
| AjnI | CCWGG | 3 cut(s) | 126, 423, 1874 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1851, 1883 |
| Alw21I | GWGCWC | 1 cut(s) | 246 |
| Alw26I | GTCTC | 5 cut(s) | 56, 372, 1169, 1201, 1691 |
| AlwI | GGATC | 1 cut(s) | 1158 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 184 |
| Ama87I | CYCGRG | 1 cut(s) | 539 |
| AoxI | GGCC | 4 cut(s) | 124, 214, 563, 794 |
| ApeKI | GCWGC | 3 cut(s) | 329, 383, 1750 |
| ApoI | RAATTY | 5 cut(s) | 26, 720, 1143, 1458, 1597 |
| Asp700I | GAANNNNTTC | 3 cut(s) | 1443, 1474, 1847 |
| Asp718I | GGTACC | 1 cut(s) | 154 |
| AspA2I | CCTAGG | 1 cut(s) | 1745 |
| AspLEI | GCGC | 3 cut(s) | 346, 1371, 1755 |
| AspS9I | GGNCC | 3 cut(s) | 421, 1642, 1871 |
| AsuHPI | GGTGA | 6 cut(s) | 599, 716, 1123, 1188, 1204, 1352 |
| AsuII | TTCGAA | 1 cut(s) | 1707 |
| AvaI | CYCGRG | 1 cut(s) | 539 |
| AvaII | GGWCC | 3 cut(s) | 421, 1642, 1871 |
| AvrII | CCTAGG | 1 cut(s) | 1745 |
| BalI | TGGCCA | 2 cut(s) | 126, 796 |
| BanI | GGYRCC | 3 cut(s) | 154, 1368, 1449 |
| BanII | GRGCYC | 1 cut(s) | 246 |
| BbsI | GAAGAC | 1 cut(s) | 342 |
| Bbv12I | GWGCWC | 1 cut(s) | 246 |
| BbvI | GCAGC | 3 cut(s) | 341, 395, 1737 |
| BccI | CCATC | 2 cut(s) | 814, 1817 |
| BceAI | ACGGC | 1 cut(s) | 822 |
| BciT130I | CCWGG | 3 cut(s) | 128, 425, 1876 |
| BciVI | GTATCC | 1 cut(s) | 402 |
| BclI | TGATCA | 1 cut(s) | 91 |
| BcoDI | GTCTC | 5 cut(s) | 56, 372, 1169, 1201, 1691 |
| BfaI | CTAG | 3 cut(s) | 80, 1244, 1746 |
| BfmI | CTRYAG | 2 cut(s) | 872, 1398 |
| BfoI | RGCGCY | 1 cut(s) | 1372 |
| BfuAI | ACCTGC | 1 cut(s) | 1610 |
| BfuI | GTATCC | 1 cut(s) | 402 |
| BglII | AGATCT | 1 cut(s) | 886 |
| BisI | GCNGC | 4 cut(s) | 214, 330, 384, 1751 |
| BlnI | CCTAGG | 1 cut(s) | 1745 |
| BlsI | GCNGC | 4 cut(s) | 215, 331, 385, 1752 |
| BmcAI | AGTACT | 2 cut(s) | 1290, 1324 |
| Bme1390I | CCNGG | 3 cut(s) | 128, 425, 1876 |
| Bme18I | GGWCC | 3 cut(s) | 421, 1642, 1871 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 272 |
| BmeT110I | CYCGRG | 1 cut(s) | 539 |
| BmgT120I | GGNCC | 3 cut(s) | 421, 1642, 1871 |
| BmiI | GGNNCC | 6 cut(s) | 156, 879, 1370, 1451, 1836, 1873 |
| BmrFI | CCNGG | 3 cut(s) | 128, 425, 1876 |
| BmsI | GCATC | 2 cut(s) | 247, 1557 |
| BpiI | GAAGAC | 1 cut(s) | 342 |
| BplI | GAGNNNNNCTC | 4 cut(s) | 153, 185, 228, 260 |
| Bpu14I | TTCGAA | 1 cut(s) | 1707 |
| BpuEI | CTTGAG | 3 cut(s) | 679, 1539, 1717 |
| BsaHI | GRCGYC | 3 cut(s) | 111, 803, 1369 |
| BsaJI | CCNNGG | 6 cut(s) | 540, 797, 1084, 1745, 1874, 1875 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1643, 1673 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 615, 874 |
| Bse1I | ACTGG | 3 cut(s) | 581, 690, 863 |
| Bse3DI | GCAATG | 2 cut(s) | 1178, 1383 |
| BseBI | CCWGG | 3 cut(s) | 128, 425, 1876 |
| BseDI | CCNNGG | 6 cut(s) | 540, 797, 1084, 1745, 1874, 1875 |
| BseGI | GGATG | 3 cut(s) | 353, 1027, 1548 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 615, 874 |
| BseMI | GCAATG | 2 cut(s) | 1178, 1383 |
| BseMII | CTCAG | 7 cut(s) | 383, 401, 1067, 1145, 1193, 1387, 1737 |
| BseNI | ACTGG | 3 cut(s) | 581, 690, 863 |
| BseRI | GAGGAG | 2 cut(s) | 1219, 1664 |
| BseXI | GCAGC | 3 cut(s) | 341, 395, 1737 |
| BsgI | GTGCAG | 1 cut(s) | 1464 |
| Bsh1236I | CGCG | 1 cut(s) | 213 |
| BshFI | GGCC | 4 cut(s) | 126, 216, 565, 796 |
| BshNI | GGYRCC | 3 cut(s) | 154, 1368, 1449 |
| BsiHKAI | GWGCWC | 1 cut(s) | 246 |
| BsiHKCI | CYCGRG | 1 cut(s) | 539 |
| BsiWI | CGTACG | 1 cut(s) | 362 |
| BslFI | GGGAC | 2 cut(s) | 851, 993 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 615, 874 |
| BsmAI | GTCTC | 5 cut(s) | 56, 372, 1169, 1201, 1691 |
| BsmBI | CGTCTC | 3 cut(s) | 56, 372, 1691 |
| BsmFI | GGGAC | 2 cut(s) | 851, 993 |
| BsmI | GAATGC | 1 cut(s) | 1028 |
| BsnI | GGCC | 4 cut(s) | 126, 216, 565, 796 |
| BsoBI | CYCGRG | 1 cut(s) | 539 |
| Bsp119I | TTCGAA | 1 cut(s) | 1707 |
| Bsp1286I | GDGCHC | 1 cut(s) | 246 |
| Bsp143I | GATC | 8 cut(s) | 46, 91, 446, 747, 784, 886, 925, 1150 |
| BspACI | CCGC | 8 cut(s) | 66, 213, 234, 305, 327, 395, 758, 1838 |
| BspANI | GGCC | 4 cut(s) | 126, 216, 565, 796 |
| BspCNI | CTCAG | 7 cut(s) | 382, 400, 1068, 1146, 1194, 1386, 1736 |
| BspFNI | CGCG | 1 cut(s) | 213 |
| BspLI | GGNNCC | 6 cut(s) | 156, 879, 1370, 1451, 1836, 1873 |
| BspMAI | CTGCAG | 1 cut(s) | 876 |
| BspMI | ACCTGC | 1 cut(s) | 1610 |
| BspPI | GGATC | 1 cut(s) | 1158 |
| BspQI | GCTCTTC | 2 cut(s) | 779, 1545 |
| BspT104I | TTCGAA | 1 cut(s) | 1707 |
| BspT107I | GGYRCC | 3 cut(s) | 154, 1368, 1449 |
| BsrDI | GCAATG | 2 cut(s) | 1178, 1383 |
| BsrI | ACTGG | 3 cut(s) | 581, 690, 863 |
| BssECI | CCNNGG | 6 cut(s) | 540, 797, 1084, 1745, 1874, 1875 |
| BssMI | GATC | 8 cut(s) | 46, 91, 446, 747, 784, 886, 925, 1150 |
| BssNAI | GTATAC | 1 cut(s) | 1379 |
| BssNI | GRCGYC | 3 cut(s) | 111, 803, 1369 |
| BssT1I | CCWWGG | 2 cut(s) | 797, 1745 |
| Bst1107I | GTATAC | 1 cut(s) | 1379 |
| Bst2UI | CCWGG | 3 cut(s) | 128, 425, 1876 |
| Bst4CI | ACNGT | 2 cut(s) | 898, 984 |
| Bst6I | CTCTTC | 4 cut(s) | 554, 779, 1449, 1545 |
| BstACI | GRCGYC | 3 cut(s) | 111, 803, 1369 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 184, 994 |
| BstBI | TTCGAA | 1 cut(s) | 1707 |
| BstC8I | GCNNGC | 4 cut(s) | 327, 844, 876, 1253 |
| BstF5I | GGATG | 3 cut(s) | 353, 1027, 1548 |
| BstFNI | CGCG | 1 cut(s) | 213 |
| BstH2I | RGCGCY | 1 cut(s) | 1372 |
| BstHHI | GCGC | 3 cut(s) | 346, 1371, 1755 |
| BstKTI | GATC | 8 cut(s) | 49, 94, 449, 750, 787, 889, 928, 1153 |
| BstMAI | GTCTC | 5 cut(s) | 56, 372, 1169, 1201, 1691 |
| BstMBI | GATC | 8 cut(s) | 46, 91, 446, 747, 784, 886, 925, 1150 |
| BstMWI | GCNNNNNNNGC | 8 cut(s) | 175, 184, 764, 802, 843, 949, 994, 1394 |
| BstNI | CCWGG | 3 cut(s) | 128, 425, 1876 |
| BstNSI | RCATGY | 3 cut(s) | 846, 1164, 1255 |
| BstSCI | CCNGG | 3 cut(s) | 126, 423, 1874 |
| BstSFI | CTRYAG | 2 cut(s) | 872, 1398 |
| BstUI | CGCG | 1 cut(s) | 213 |
| BstV1I | GCAGC | 3 cut(s) | 341, 395, 1737 |
| BstV2I | GAAGAC | 1 cut(s) | 342 |
| BstX2I | RGATCY | 2 cut(s) | 886, 1150 |
| BstYI | RGATCY | 2 cut(s) | 886, 1150 |
| BstZ17I | GTATAC | 1 cut(s) | 1379 |
| BsuI | GTATCC | 1 cut(s) | 402 |
| BsuRI | GGCC | 4 cut(s) | 126, 216, 565, 796 |
| BtsCI | GGATG | 3 cut(s) | 353, 1027, 1548 |
| BtsI | GCAGTG | 3 cut(s) | 339, 1517, 1677 |
| BtsIMutI | CAGTG | 6 cut(s) | 339, 436, 697, 1199, 1517, 1677 |
| BveI | ACCTGC | 1 cut(s) | 1610 |
| Cac8I | GCNNGC | 4 cut(s) | 327, 844, 876, 1253 |
| CaiI | CAGNNNCTG | 1 cut(s) | 184 |
| CfoI | GCGC | 3 cut(s) | 346, 1371, 1755 |
| Cfr13I | GGNCC | 3 cut(s) | 421, 1642, 1871 |
| CseI | GACGC | 5 cut(s) | 276, 365, 811, 1673, 1862 |
| CsiI | ACCWGGT | 1 cut(s) | 423 |
| Csp6I | GTAC | 5 cut(s) | 155, 363, 1289, 1323, 1565 |
| CviQI | GTAC | 5 cut(s) | 155, 363, 1289, 1323, 1565 |
| DinI | GGCGCC | 1 cut(s) | 1370 |
| DpnI | GATC | 8 cut(s) | 48, 93, 448, 749, 786, 888, 927, 1152 |
| DpnII | GATC | 8 cut(s) | 46, 91, 446, 747, 784, 886, 925, 1150 |
| DraI | TTTAAA | 1 cut(s) | 1602 |
| DriI | GACNNNNNGTC | 1 cut(s) | 272 |
| EaeI | YGGCCR | 2 cut(s) | 124, 794 |
| Eam1104I | CTCTTC | 4 cut(s) | 554, 779, 1449, 1545 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 272 |
| EarI | CTCTTC | 4 cut(s) | 554, 779, 1449, 1545 |
| EciI | GGCGGA | 1 cut(s) | 773 |
| Ecl136II | GAGCTC | 1 cut(s) | 244 |
| Eco130I | CCWWGG | 2 cut(s) | 797, 1745 |
| Eco24I | GRGCYC | 1 cut(s) | 246 |
| Eco32I | GATATC | 1 cut(s) | 1192 |
| Eco47I | GGWCC | 3 cut(s) | 421, 1642, 1871 |
| Eco53kI | GAGCTC | 1 cut(s) | 244 |
| Eco57I | CTGAAG | 5 cut(s) | 354, 903, 974, 1206, 1878 |
| Eco88I | CYCGRG | 1 cut(s) | 539 |
| EcoICRI | GAGCTC | 1 cut(s) | 244 |
| EcoRII | CCWGG | 3 cut(s) | 126, 423, 1874 |
| EcoRV | GATATC | 1 cut(s) | 1192 |
| EcoT14I | CCWWGG | 2 cut(s) | 797, 1745 |
| EcoT38I | GRGCYC | 1 cut(s) | 246 |
| EgeI | GGCGCC | 1 cut(s) | 1370 |
| EheI | GGCGCC | 1 cut(s) | 1370 |
| ErhI | CCWWGG | 2 cut(s) | 797, 1745 |
| Esp3I | CGTCTC | 3 cut(s) | 56, 372, 1691 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 500, 532 |
| FaqI | GGGAC | 2 cut(s) | 851, 993 |
| FauI | CCCGC | 1 cut(s) | 334 |
| FauNDI | CATATG | 1 cut(s) | 741 |
| FbaI | TGATCA | 1 cut(s) | 91 |
| FblI | GTMKAC | 3 cut(s) | 5, 909, 1378 |
| Fnu4HI | GCNGC | 4 cut(s) | 214, 330, 384, 1751 |
| FokI | GGATG | 3 cut(s) | 340, 1034, 1535 |
| FriOI | GRGCYC | 1 cut(s) | 246 |
| Fsp4HI | GCNGC | 4 cut(s) | 214, 330, 384, 1751 |
| FspBI | CTAG | 3 cut(s) | 80, 1244, 1746 |
| GlaI | GCGC | 3 cut(s) | 345, 1370, 1754 |
| GluI | GCNGC | 4 cut(s) | 214, 330, 384, 1751 |
| HaeII | RGCGCY | 1 cut(s) | 1372 |
| HaeIII | GGCC | 4 cut(s) | 126, 216, 565, 796 |
| HgaI | GACGC | 5 cut(s) | 276, 365, 811, 1673, 1862 |
| HhaI | GCGC | 3 cut(s) | 346, 1371, 1755 |
| Hin1I | GRCGYC | 3 cut(s) | 111, 803, 1369 |
| Hin6I | GCGC | 3 cut(s) | 344, 1369, 1753 |
| HinP1I | GCGC | 3 cut(s) | 344, 1369, 1753 |
| HindIII | AAGCTT | 2 cut(s) | 765, 1628 |
| HphI | GGTGA | 6 cut(s) | 599, 716, 1123, 1188, 1204, 1352 |
| Hpy166II | GTNNAC | 4 cut(s) | 6, 894, 910, 1379 |
| Hpy188I | TCNGA | 9 cut(s) | 22, 56, 109, 930, 1056, 1077, 1085, 1155, 1231 |
| Hpy188III | TCNNGA | 9 cut(s) | 12, 491, 658, 881, 923, 1148, 1429, 1736, 1789 |
| Hpy8I | GTNNAC | 4 cut(s) | 6, 894, 910, 1379 |
| Hpy99I | CGWCG | 1 cut(s) | 1856 |
| HpyAV | CCTTC | 5 cut(s) | 468, 632, 658, 952, 1122 |
| HpyCH4III | ACNGT | 2 cut(s) | 898, 984 |
| HpyCH4IV | ACGT | 3 cut(s) | 111, 365, 934 |
| HpyCH4V | TGCA | 6 cut(s) | 842, 874, 965, 1332, 1481, 1522 |
| HpyF10VI | GCNNNNNNNGC | 8 cut(s) | 175, 184, 764, 802, 843, 949, 994, 1394 |
| HpySE526I | ACGT | 3 cut(s) | 111, 365, 934 |
| Hsp92I | GRCGYC | 3 cut(s) | 111, 803, 1369 |
| HspAI | GCGC | 3 cut(s) | 344, 1369, 1753 |
| KasI | GGCGCC | 1 cut(s) | 1368 |
| KpnI | GGTACC | 1 cut(s) | 158 |
| Ksp22I | TGATCA | 1 cut(s) | 91 |
| Kzo9I | GATC | 8 cut(s) | 46, 91, 446, 747, 784, 886, 925, 1150 |
| LguI | GCTCTTC | 2 cut(s) | 779, 1545 |
| LmnI | GCTCC | 5 cut(s) | 130, 175, 477, 883, 1651 |
| Lsp1109I | GCAGC | 3 cut(s) | 341, 395, 1737 |
| LweI | GCATC | 2 cut(s) | 247, 1557 |
| MabI | ACCWGGT | 1 cut(s) | 423 |
| MaeI | CTAG | 3 cut(s) | 80, 1244, 1746 |
| MaeII | ACGT | 3 cut(s) | 111, 365, 934 |
| MaeIII | GTNAC | 5 cut(s) | 1163, 1235, 1718, 1775, 1802 |
| MalI | GATC | 8 cut(s) | 48, 93, 448, 749, 786, 888, 927, 1152 |
| MboI | GATC | 8 cut(s) | 46, 91, 446, 747, 784, 886, 925, 1150 |
| MfeI | CAATTG | 3 cut(s) | 681, 702, 974 |
| MflI | RGATCY | 2 cut(s) | 886, 1150 |
| MhlI | GDGCHC | 1 cut(s) | 246 |
| MlsI | TGGCCA | 2 cut(s) | 126, 796 |
| MluNI | TGGCCA | 2 cut(s) | 126, 796 |
| Mly113I | GGCGCC | 1 cut(s) | 1369 |
| MlyI | GAGTC | 7 cut(s) | 171, 175, 539, 833, 1203, 1505, 1715 |
| Mox20I | TGGCCA | 2 cut(s) | 126, 796 |
| MroXI | GAANNNNTTC | 3 cut(s) | 1443, 1474, 1847 |
| MscI | TGGCCA | 2 cut(s) | 126, 796 |
| MseI | TTAA | 5 cut(s) | 102, 189, 1125, 1364, 1601 |
| MslI | CAYNNNNRTG | 2 cut(s) | 406, 1013 |
| Msp20I | TGGCCA | 2 cut(s) | 126, 796 |
| MspA1I | CMGCKG | 3 cut(s) | 68, 332, 397 |
| MspR9I | CCNGG | 3 cut(s) | 128, 425, 1876 |
| MunI | CAATTG | 3 cut(s) | 681, 702, 974 |
| Mva1269I | GAATGC | 1 cut(s) | 1028 |
| MvaI | CCWGG | 3 cut(s) | 128, 425, 1876 |
| MvnI | CGCG | 1 cut(s) | 213 |
| MwoI | GCNNNNNNNGC | 8 cut(s) | 175, 184, 764, 802, 843, 949, 994, 1394 |
| NarI | GGCGCC | 1 cut(s) | 1369 |
| NdeI | CATATG | 1 cut(s) | 741 |
| NdeII | GATC | 8 cut(s) | 46, 91, 446, 747, 784, 886, 925, 1150 |
| NlaIV | GGNNCC | 6 cut(s) | 156, 879, 1370, 1451, 1836, 1873 |
| NmuCI | GTSAC | 2 cut(s) | 1163, 1718 |
| NspI | RCATGY | 3 cut(s) | 846, 1164, 1255 |
| NspV | TTCGAA | 1 cut(s) | 1707 |
| PaeI | GCATGC | 2 cut(s) | 846, 1255 |
| PaqCI | CACCTGC | 1 cut(s) | 1610 |
| PasI | CCCWGGG | 1 cut(s) | 1875 |
| PciSI | GCTCTTC | 2 cut(s) | 779, 1545 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 1227 |
| PctI | GAATGC | 1 cut(s) | 1028 |
| PdmI | GAANNNNTTC | 3 cut(s) | 1443, 1474, 1847 |
| PfeI | GAWTC | 6 cut(s) | 350, 642, 733, 1439, 1732, 1827 |
| Pfl23II | CGTACG | 1 cut(s) | 362 |
| PflFI | GACNNNGTC | 1 cut(s) | 425 |
| PkrI | GCNGC | 4 cut(s) | 215, 331, 385, 1752 |
| PleI | GAGTC | 7 cut(s) | 170, 175, 539, 832, 1203, 1504, 1715 |
| PluTI | GGCGCC | 1 cut(s) | 1372 |
| PpsI | GAGTC | 7 cut(s) | 170, 175, 539, 832, 1203, 1504, 1715 |
| Psp124BI | GAGCTC | 1 cut(s) | 246 |
| Psp6I | CCWGG | 3 cut(s) | 126, 423, 1874 |
| PspGI | CCWGG | 3 cut(s) | 126, 423, 1874 |
| PspLI | CGTACG | 1 cut(s) | 362 |
| PspN4I | GGNNCC | 6 cut(s) | 156, 879, 1370, 1451, 1836, 1873 |
| PspPI | GGNCC | 3 cut(s) | 421, 1642, 1871 |
| PstI | CTGCAG | 1 cut(s) | 876 |
| PstNI | CAGNNNCTG | 1 cut(s) | 184 |
| PsuI | RGATCY | 2 cut(s) | 886, 1150 |
| PsyI | GACNNNGTC | 1 cut(s) | 425 |
| PvuII | CAGCTG | 1 cut(s) | 332 |
| RsaI | GTAC | 5 cut(s) | 156, 364, 1290, 1324, 1566 |
| RsaNI | GTAC | 5 cut(s) | 155, 363, 1289, 1323, 1565 |
| RseI | CAYNNNNRTG | 2 cut(s) | 406, 1013 |
| SacI | GAGCTC | 1 cut(s) | 246 |
| SapI | GCTCTTC | 2 cut(s) | 779, 1545 |
| SaqAI | TTAA | 5 cut(s) | 102, 189, 1125, 1364, 1601 |
| SatI | GCNGC | 4 cut(s) | 214, 330, 384, 1751 |
| Sau3AI | GATC | 8 cut(s) | 46, 91, 446, 747, 784, 886, 925, 1150 |
| Sau96I | GGNCC | 3 cut(s) | 421, 1642, 1871 |
| ScaI | AGTACT | 2 cut(s) | 1290, 1324 |
| SchI | GAGTC | 7 cut(s) | 171, 175, 539, 833, 1203, 1505, 1715 |
| ScrFI | CCNGG | 3 cut(s) | 128, 425, 1876 |
| SduI | GDGCHC | 1 cut(s) | 246 |
| SexAI | ACCWGGT | 1 cut(s) | 423 |
| SfaNI | GCATC | 2 cut(s) | 247, 1557 |
| SfcI | CTRYAG | 2 cut(s) | 872, 1398 |
| SfoI | GGCGCC | 1 cut(s) | 1370 |
| SfuI | TTCGAA | 1 cut(s) | 1707 |
| SinI | GGWCC | 3 cut(s) | 421, 1642, 1871 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 406, 1013 |
| SmlI | CTYRAG | 4 cut(s) | 10, 658, 1554, 1696 |
| SmoI | CTYRAG | 4 cut(s) | 10, 658, 1554, 1696 |
| SphI | GCATGC | 2 cut(s) | 846, 1255 |
| SsiI | CCGC | 8 cut(s) | 66, 213, 234, 305, 327, 395, 758, 1838 |
| SspDI | GGCGCC | 1 cut(s) | 1368 |
| SspI | AATATT | 1 cut(s) | 534 |
| SspMI | CTAG | 3 cut(s) | 80, 1244, 1746 |
| SstI | GAGCTC | 1 cut(s) | 246 |
| StyD4I | CCNGG | 3 cut(s) | 126, 423, 1874 |
| StyI | CCWWGG | 2 cut(s) | 797, 1745 |
| TaaI | ACNGT | 2 cut(s) | 898, 984 |
| TaiI | ACGT | 3 cut(s) | 114, 368, 937 |
| TaqI | TCGA | 9 cut(s) | 87, 252, 787, 1221, 1405, 1707, 1788, 1818, 1851 |
| TatI | WGTACW | 2 cut(s) | 1288, 1322 |
| TauI | GCSGC | 1 cut(s) | 216 |
| TfiI | GAWTC | 6 cut(s) | 350, 642, 733, 1439, 1732, 1827 |
| Tru1I | TTAA | 5 cut(s) | 102, 189, 1125, 1364, 1601 |
| Tru9I | TTAA | 5 cut(s) | 102, 189, 1125, 1364, 1601 |
| TscAI | CASTG | 6 cut(s) | 346, 436, 697, 1206, 1524, 1677 |
| TseFI | GTSAC | 2 cut(s) | 1163, 1718 |
| TseI | GCWGC | 3 cut(s) | 329, 383, 1750 |
| Tsp45I | GTSAC | 2 cut(s) | 1163, 1718 |
| TspDTI | ATGAA | 7 cut(s) | 19, 329, 498, 586, 1038, 1452, 1500 |
| TspRI | CASTG | 6 cut(s) | 346, 436, 697, 1206, 1524, 1677 |
| Tth111I | GACNNNGTC | 1 cut(s) | 425 |
| VpaK11BI | GGWCC | 3 cut(s) | 421, 1642, 1871 |
| XapI | RAATTY | 5 cut(s) | 26, 720, 1143, 1458, 1597 |
| XceI | RCATGY | 3 cut(s) | 846, 1164, 1255 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 573 |
| XmaJI | CCTAGG | 1 cut(s) | 1745 |
| XmiI | GTMKAC | 3 cut(s) | 5, 909, 1378 |
| XmnI | GAANNNNTTC | 3 cut(s) | 1443, 1474, 1847 |
| XspI | CTAG | 3 cut(s) | 80, 1244, 1746 |
| ZraI | GACGTC | 1 cut(s) | 112 |
| ZrmI | AGTACT | 2 cut(s) | 1290, 1324 |