Encoded by
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 388 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 177, 1180 |
| AccII | CGCG | 3 cut(s) | 285, 385, 1095 |
| AciI | CCGC | 9 cut(s) | 77, 98, 220, 283, 1093, 1095, 1378, 1459, 1768 |
| AclWI | GGATC | 7 cut(s) | 86, 115, 128, 237, 250, 287, 300 |
| AcoI | YGGCCR | 2 cut(s) | 78, 601 |
| AcsI | RAATTY | 1 cut(s) | 1152 |
| AcuI | CTGAAG | 5 cut(s) | 542, 646, 1088, 1107, 1698 |
| AfaI | GTAC | 5 cut(s) | 715, 733, 826, 1587, 1842 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 76, 177, 289, 340, 1180, 1767 |
| AflII | CTTAAG | 1 cut(s) | 1133 |
| AflIII | ACRYGT | 2 cut(s) | 364, 383 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 256 |
| AjiI | CACGTC | 1 cut(s) | 445 |
| AjnI | CCWGG | 2 cut(s) | 246, 603 |
| AleI | CACNNNNGTG | 1 cut(s) | 427 |
| Alw21I | GWGCWC | 2 cut(s) | 931, 1066 |
| Alw26I | GTCTC | 7 cut(s) | 62, 396, 438, 626, 1513, 1667, 1709 |
| AlwI | GGATC | 7 cut(s) | 86, 115, 128, 237, 250, 287, 300 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 872 |
| AoxI | GGCC | 3 cut(s) | 78, 578, 601 |
| ApeKI | GCWGC | 4 cut(s) | 154, 157, 470, 1747 |
| ApoI | RAATTY | 1 cut(s) | 1152 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1452 |
| AspS9I | GGNCC | 1 cut(s) | 1322 |
| AsuC2I | CCSGG | 1 cut(s) | 70 |
| AsuHPI | GGTGA | 1 cut(s) | 1361 |
| AsuII | TTCGAA | 1 cut(s) | 303 |
| AvaII | GGWCC | 1 cut(s) | 1322 |
| BalI | TGGCCA | 1 cut(s) | 603 |
| BamHI | GGATCC | 3 cut(s) | 120, 242, 292 |
| BanII | GRGCYC | 1 cut(s) | 1066 |
| BbsI | GAAGAC | 2 cut(s) | 652, 977 |
| Bbv12I | GWGCWC | 2 cut(s) | 931, 1066 |
| BbvI | GCAGC | 4 cut(s) | 141, 144, 482, 1759 |
| BccI | CCATC | 5 cut(s) | 361, 1046, 1412, 1770, 1800 |
| BceAI | ACGGC | 2 cut(s) | 90, 268 |
| BciT130I | CCWGG | 2 cut(s) | 248, 605 |
| BcnI | CCSGG | 1 cut(s) | 70 |
| BcoDI | GTCTC | 7 cut(s) | 62, 396, 438, 626, 1513, 1667, 1709 |
| BfaI | CTAG | 3 cut(s) | 1157, 1355, 1605 |
| BfmI | CTRYAG | 2 cut(s) | 636, 1339 |
| BfrI | CTTAAG | 1 cut(s) | 1133 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 74 |
| BglII | AGATCT | 1 cut(s) | 1159 |
| BisI | GCNGC | 8 cut(s) | 78, 155, 158, 220, 471, 1379, 1459, 1748 |
| BlpI | GCTNAGC | 3 cut(s) | 491, 783, 1382 |
| BlsI | GCNGC | 8 cut(s) | 79, 156, 159, 221, 472, 1380, 1460, 1749 |
| Bme1390I | CCNGG | 3 cut(s) | 70, 248, 605 |
| Bme18I | GGWCC | 1 cut(s) | 1322 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 256 |
| BmgBI | CACGTC | 1 cut(s) | 445 |
| BmgT120I | GGNCC | 1 cut(s) | 1322 |
| BmiI | GGNNCC | 3 cut(s) | 122, 244, 294 |
| BmrFI | CCNGG | 3 cut(s) | 70, 248, 605 |
| BmsI | GCATC | 6 cut(s) | 13, 276, 339, 522, 1578, 1798 |
| BpiI | GAAGAC | 2 cut(s) | 652, 977 |
| BpmI | CTGGAG | 3 cut(s) | 867, 971, 1745 |
| Bpu1102I | GCTNAGC | 3 cut(s) | 491, 783, 1382 |
| Bpu14I | TTCGAA | 1 cut(s) | 303 |
| BpuEI | CTTGAG | 1 cut(s) | 1691 |
| BpuMI | CCSGG | 1 cut(s) | 70 |
| BsaI | GGTCTC | 2 cut(s) | 438, 1513 |
| BsaJI | CCNNGG | 5 cut(s) | 210, 246, 604, 743, 1093 |
| BsaWI | WCCGGW | 1 cut(s) | 1540 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 642, 672 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 76, 177, 289, 340, 1180, 1767 |
| Bse1I | ACTGG | 4 cut(s) | 199, 444, 711, 866 |
| BseBI | CCWGG | 2 cut(s) | 248, 605 |
| BseDI | CCNNGG | 5 cut(s) | 210, 246, 604, 743, 1093 |
| BseGI | GGATG | 5 cut(s) | 739, 1039, 1237, 1569, 1813 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 76, 177, 289, 340, 1180, 1767 |
| BseMII | CTCAG | 9 cut(s) | 73, 363, 407, 425, 482, 774, 864, 1154, 1396 |
| BseNI | ACTGG | 4 cut(s) | 199, 444, 711, 866 |
| BseRI | GAGGAG | 1 cut(s) | 656 |
| BseXI | GCAGC | 4 cut(s) | 141, 144, 482, 1759 |
| BseYI | CCCAGC | 2 cut(s) | 49, 991 |
| BsgI | GTGCAG | 1 cut(s) | 489 |
| Bsh1236I | CGCG | 3 cut(s) | 285, 385, 1095 |
| BshFI | GGCC | 3 cut(s) | 80, 580, 603 |
| BsiHKAI | GWGCWC | 2 cut(s) | 931, 1066 |
| BsiSI | CCGG | 3 cut(s) | 70, 95, 1541 |
| BslFI | GGGAC | 2 cut(s) | 854, 1008 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 76, 177, 289, 340, 1180, 1767 |
| BsmAI | GTCTC | 7 cut(s) | 62, 396, 438, 626, 1513, 1667, 1709 |
| BsmBI | CGTCTC | 3 cut(s) | 62, 396, 1709 |
| BsmFI | GGGAC | 2 cut(s) | 854, 1008 |
| BsmI | GAATGC | 1 cut(s) | 36 |
| BsnI | GGCC | 3 cut(s) | 80, 580, 603 |
| Bso31I | GGTCTC | 2 cut(s) | 438, 1513 |
| Bsp119I | TTCGAA | 1 cut(s) | 303 |
| Bsp1286I | GDGCHC | 2 cut(s) | 931, 1066 |
| Bsp1407I | TGTACA | 1 cut(s) | 731 |
| Bsp143I | GATC | 8 cut(s) | 91, 120, 242, 292, 546, 960, 1159, 1351 |
| Bsp1720I | GCTNAGC | 3 cut(s) | 491, 783, 1382 |
| BspACI | CCGC | 9 cut(s) | 77, 98, 220, 283, 1093, 1095, 1378, 1459, 1768 |
| BspANI | GGCC | 3 cut(s) | 80, 580, 603 |
| BspCNI | CTCAG | 9 cut(s) | 72, 364, 406, 424, 483, 775, 865, 1155, 1395 |
| BspFNI | CGCG | 3 cut(s) | 285, 385, 1095 |
| BspHI | TCATGA | 4 cut(s) | 141, 400, 672, 775 |
| BspLI | GGNNCC | 3 cut(s) | 122, 244, 294 |
| BspMAI | CTGCAG | 2 cut(s) | 640, 1343 |
| BspPI | GGATC | 7 cut(s) | 86, 115, 128, 237, 250, 287, 300 |
| BspQI | GCTCTTC | 1 cut(s) | 123 |
| BspT104I | TTCGAA | 1 cut(s) | 303 |
| BspTI | CTTAAG | 1 cut(s) | 1133 |
| BspTNI | GGTCTC | 2 cut(s) | 438, 1513 |
| BsrGI | TGTACA | 1 cut(s) | 731 |
| BsrI | ACTGG | 4 cut(s) | 199, 444, 711, 866 |
| BssECI | CCNNGG | 5 cut(s) | 210, 246, 604, 743, 1093 |
| BssMI | GATC | 8 cut(s) | 91, 120, 242, 292, 546, 960, 1159, 1351 |
| BssT1I | CCWWGG | 2 cut(s) | 210, 743 |
| Bst2UI | CCWGG | 2 cut(s) | 248, 605 |
| Bst4CI | ACNGT | 6 cut(s) | 177, 913, 982, 999, 1518, 1743 |
| Bst6I | CTCTTC | 5 cut(s) | 123, 569, 1425, 1458, 1674 |
| BstAFI | CTTAAG | 1 cut(s) | 1133 |
| BstAUI | TGTACA | 1 cut(s) | 731 |
| BstBI | TTCGAA | 1 cut(s) | 303 |
| BstC8I | GCNNGC | 3 cut(s) | 287, 847, 1171 |
| BstDEI | CTNAG | 9 cut(s) | 59, 372, 393, 411, 491, 783, 873, 1163, 1382 |
| BstDSI | CCRYGG | 1 cut(s) | 1093 |
| BstF5I | GGATG | 5 cut(s) | 739, 1039, 1237, 1569, 1813 |
| BstFNI | CGCG | 3 cut(s) | 285, 385, 1095 |
| BstKTI | GATC | 8 cut(s) | 94, 123, 245, 295, 549, 963, 1162, 1354 |
| BstMAI | GTCTC | 7 cut(s) | 62, 396, 438, 626, 1513, 1667, 1709 |
| BstMBI | GATC | 8 cut(s) | 91, 120, 242, 292, 546, 960, 1159, 1351 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 74, 779 |
| BstNI | CCWGG | 2 cut(s) | 248, 605 |
| BstNSI | RCATGY | 4 cut(s) | 154, 681, 760, 849 |
| BstSCI | CCNGG | 3 cut(s) | 68, 246, 603 |
| BstSFI | CTRYAG | 2 cut(s) | 636, 1339 |
| BstUI | CGCG | 3 cut(s) | 285, 385, 1095 |
| BstV1I | GCAGC | 4 cut(s) | 141, 144, 482, 1759 |
| BstV2I | GAAGAC | 2 cut(s) | 652, 977 |
| BstX2I | RGATCY | 4 cut(s) | 120, 242, 292, 1159 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 49 |
| BstYI | RGATCY | 4 cut(s) | 120, 242, 292, 1159 |
| BsuRI | GGCC | 3 cut(s) | 80, 580, 603 |
| BtgI | CCRYGG | 1 cut(s) | 1093 |
| BtgZI | GCGATG | 1 cut(s) | 737 |
| BtrI | CACGTC | 1 cut(s) | 445 |
| BtsCI | GGATG | 5 cut(s) | 739, 1039, 1237, 1569, 1813 |
| BtsIMutI | CAGTG | 1 cut(s) | 182 |
| Cac8I | GCNNGC | 3 cut(s) | 287, 847, 1171 |
| CaiI | CAGNNNCTG | 1 cut(s) | 872 |
| CciI | TCATGA | 4 cut(s) | 141, 400, 672, 775 |
| Cfr13I | GGNCC | 1 cut(s) | 1322 |
| Cfr42I | CCGCGG | 1 cut(s) | 1096 |
| CseI | GACGC | 3 cut(s) | 391, 696, 1691 |
| Csp6I | GTAC | 5 cut(s) | 714, 732, 825, 1586, 1841 |
| CviQI | GTAC | 5 cut(s) | 714, 732, 825, 1586, 1841 |
| DdeI | CTNAG | 9 cut(s) | 59, 372, 393, 411, 491, 783, 873, 1163, 1382 |
| DpnI | GATC | 8 cut(s) | 93, 122, 244, 294, 548, 962, 1161, 1353 |
| DpnII | GATC | 8 cut(s) | 91, 120, 242, 292, 546, 960, 1159, 1351 |
| DraI | TTTAAA | 2 cut(s) | 702, 1662 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 388 |
| DriI | GACNNNNNGTC | 1 cut(s) | 256 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 388 |
| EaeI | YGGCCR | 2 cut(s) | 78, 601 |
| Eam1104I | CTCTTC | 5 cut(s) | 123, 569, 1425, 1458, 1674 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 256 |
| EarI | CTCTTC | 5 cut(s) | 123, 569, 1425, 1458, 1674 |
| Ecl136II | GAGCTC | 1 cut(s) | 1064 |
| Eco130I | CCWWGG | 2 cut(s) | 210, 743 |
| Eco147I | AGGCCT | 1 cut(s) | 580 |
| Eco24I | GRGCYC | 1 cut(s) | 1066 |
| Eco31I | GGTCTC | 2 cut(s) | 438, 1513 |
| Eco32I | GATATC | 1 cut(s) | 1201 |
| Eco47I | GGWCC | 1 cut(s) | 1322 |
| Eco53kI | GAGCTC | 1 cut(s) | 1064 |
| Eco57I | CTGAAG | 5 cut(s) | 542, 646, 1088, 1107, 1698 |
| EcoICRI | GAGCTC | 1 cut(s) | 1064 |
| EcoRII | CCWGG | 2 cut(s) | 246, 603 |
| EcoRV | GATATC | 1 cut(s) | 1201 |
| EcoT14I | CCWWGG | 2 cut(s) | 210, 743 |
| EcoT22I | ATGCAT | 2 cut(s) | 38, 1813 |
| EcoT38I | GRGCYC | 1 cut(s) | 1066 |
| ErhI | CCWWGG | 2 cut(s) | 210, 743 |
| Esp3I | CGTCTC | 3 cut(s) | 62, 396, 1709 |
| FalI | AAGNNNNNCTT | 6 cut(s) | 117, 149, 221, 253, 771, 803 |
| FaqI | GGGAC | 2 cut(s) | 854, 1008 |
| FauNDI | CATATG | 1 cut(s) | 1813 |
| Fnu4HI | GCNGC | 8 cut(s) | 78, 155, 158, 220, 471, 1379, 1459, 1748 |
| FokI | GGATG | 5 cut(s) | 726, 1046, 1244, 1556, 1820 |
| FriOI | GRGCYC | 1 cut(s) | 1066 |
| Fsp4HI | GCNGC | 8 cut(s) | 78, 155, 158, 220, 471, 1379, 1459, 1748 |
| FspBI | CTAG | 3 cut(s) | 1157, 1355, 1605 |
| GluI | GCNGC | 8 cut(s) | 78, 155, 158, 220, 471, 1379, 1459, 1748 |
| GsaI | CCCAGC | 2 cut(s) | 53, 995 |
| GsuI | CTGGAG | 3 cut(s) | 867, 971, 1745 |
| HaeIII | GGCC | 3 cut(s) | 80, 580, 603 |
| HapII | CCGG | 3 cut(s) | 70, 95, 1541 |
| HgaI | GACGC | 3 cut(s) | 391, 696, 1691 |
| HincII | GTYRAC | 2 cut(s) | 6, 1104 |
| HindII | GTYRAC | 2 cut(s) | 6, 1104 |
| HinfI | GANTC | 7 cut(s) | 257, 344, 374, 397, 688, 748, 1448 |
| HpaII | CCGG | 3 cut(s) | 70, 95, 1541 |
| HphI | GGTGA | 1 cut(s) | 1361 |
| Hpy166II | GTNNAC | 2 cut(s) | 6, 1104 |
| Hpy8I | GTNNAC | 2 cut(s) | 6, 1104 |
| Hpy99I | CGWCG | 2 cut(s) | 92, 314 |
| HpyAV | CCTTC | 4 cut(s) | 811, 1131, 1203, 1298 |
| HpyCH4III | ACNGT | 6 cut(s) | 177, 913, 982, 999, 1518, 1743 |
| HpyCH4IV | ACGT | 3 cut(s) | 309, 366, 444 |
| HpyCH4V | TGCA | 9 cut(s) | 26, 36, 470, 638, 681, 845, 1341, 1747, 1811 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 74, 779 |
| HpyF3I | CTNAG | 9 cut(s) | 59, 372, 393, 411, 491, 783, 873, 1163, 1382 |
| HpySE526I | ACGT | 3 cut(s) | 309, 366, 444 |
| KspI | CCGCGG | 1 cut(s) | 1096 |
| Kzo9I | GATC | 8 cut(s) | 91, 120, 242, 292, 546, 960, 1159, 1351 |
| LguI | GCTCTTC | 1 cut(s) | 123 |
| LmnI | GCTCC | 6 cut(s) | 181, 1061, 1122, 1492, 1648, 1833 |
| Lsp1109I | GCAGC | 4 cut(s) | 141, 144, 482, 1759 |
| LweI | GCATC | 6 cut(s) | 13, 276, 339, 522, 1578, 1798 |
| MaeI | CTAG | 3 cut(s) | 1157, 1355, 1605 |
| MaeII | ACGT | 3 cut(s) | 309, 366, 444 |
| MaeIII | GTNAC | 4 cut(s) | 420, 613, 876, 1244 |
| MalI | GATC | 8 cut(s) | 93, 122, 244, 294, 548, 962, 1161, 1353 |
| MboI | GATC | 8 cut(s) | 91, 120, 242, 292, 546, 960, 1159, 1351 |
| MfeI | CAATTG | 1 cut(s) | 1107 |
| MflI | RGATCY | 4 cut(s) | 120, 242, 292, 1159 |
| MhlI | GDGCHC | 2 cut(s) | 931, 1066 |
| MlsI | TGGCCA | 1 cut(s) | 603 |
| MluI | ACGCGT | 1 cut(s) | 383 |
| MluNI | TGGCCA | 1 cut(s) | 603 |
| MlyI | GAGTC | 4 cut(s) | 266, 353, 383, 406 |
| MmeI | TCCRAC | 2 cut(s) | 1407, 1416 |
| Mox20I | TGGCCA | 1 cut(s) | 603 |
| Mph1103I | ATGCAT | 2 cut(s) | 38, 1813 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1452 |
| MscI | TGGCCA | 1 cut(s) | 603 |
| MseI | TTAA | 6 cut(s) | 701, 851, 1134, 1413, 1622, 1661 |
| MslI | CAYNNNNRTG | 6 cut(s) | 427, 702, 761, 1028, 1230, 1391 |
| Msp20I | TGGCCA | 1 cut(s) | 603 |
| MspA1I | CMGCKG | 3 cut(s) | 887, 991, 1095 |
| MspCI | CTTAAG | 1 cut(s) | 1133 |
| MspI | CCGG | 3 cut(s) | 70, 95, 1541 |
| MspR9I | CCNGG | 3 cut(s) | 70, 248, 605 |
| MunI | CAATTG | 1 cut(s) | 1107 |
| Mva1269I | GAATGC | 1 cut(s) | 36 |
| MvaI | CCWGG | 2 cut(s) | 248, 605 |
| MvnI | CGCG | 3 cut(s) | 285, 385, 1095 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 74, 779 |
| NciI | CCSGG | 1 cut(s) | 70 |
| NdeI | CATATG | 1 cut(s) | 1813 |
| NdeII | GATC | 8 cut(s) | 91, 120, 242, 292, 546, 960, 1159, 1351 |
| NlaIV | GGNNCC | 3 cut(s) | 122, 244, 294 |
| NmuCI | GTSAC | 1 cut(s) | 420 |
| NsiI | ATGCAT | 2 cut(s) | 38, 1813 |
| NspI | RCATGY | 4 cut(s) | 154, 681, 760, 849 |
| NspV | TTCGAA | 1 cut(s) | 303 |
| OliI | CACNNNNGTG | 1 cut(s) | 427 |
| PaeI | GCATGC | 1 cut(s) | 849 |
| PagI | TCATGA | 4 cut(s) | 141, 400, 672, 775 |
| PceI | AGGCCT | 1 cut(s) | 580 |
| PciSI | GCTCTTC | 1 cut(s) | 123 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 309 |
| PctI | GAATGC | 1 cut(s) | 36 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1452 |
| PfeI | GAWTC | 3 cut(s) | 688, 748, 1448 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 177, 1180 |
| PkrI | GCNGC | 8 cut(s) | 79, 156, 159, 221, 472, 1380, 1460, 1749 |
| PleI | GAGTC | 4 cut(s) | 265, 352, 382, 405 |
| PpsI | GAGTC | 4 cut(s) | 265, 352, 382, 405 |
| Psp124BI | GAGCTC | 1 cut(s) | 1066 |
| Psp6I | CCWGG | 2 cut(s) | 246, 603 |
| PspFI | CCCAGC | 2 cut(s) | 49, 991 |
| PspGI | CCWGG | 2 cut(s) | 246, 603 |
| PspN4I | GGNNCC | 3 cut(s) | 122, 244, 294 |
| PspPI | GGNCC | 1 cut(s) | 1322 |
| PstI | CTGCAG | 2 cut(s) | 640, 1343 |
| PstNI | CAGNNNCTG | 1 cut(s) | 872 |
| PsuI | RGATCY | 4 cut(s) | 120, 242, 292, 1159 |
| PvuII | CAGCTG | 2 cut(s) | 887, 991 |
| RsaI | GTAC | 5 cut(s) | 715, 733, 826, 1587, 1842 |
| RsaNI | GTAC | 5 cut(s) | 714, 732, 825, 1586, 1841 |
| RseI | CAYNNNNRTG | 6 cut(s) | 427, 702, 761, 1028, 1230, 1391 |
| SacI | GAGCTC | 1 cut(s) | 1066 |
| SacII | CCGCGG | 1 cut(s) | 1096 |
| SapI | GCTCTTC | 1 cut(s) | 123 |
| SaqAI | TTAA | 6 cut(s) | 701, 851, 1134, 1413, 1622, 1661 |
| SatI | GCNGC | 8 cut(s) | 78, 155, 158, 220, 471, 1379, 1459, 1748 |
| Sau3AI | GATC | 8 cut(s) | 91, 120, 242, 292, 546, 960, 1159, 1351 |
| Sau96I | GGNCC | 1 cut(s) | 1322 |
| SchI | GAGTC | 4 cut(s) | 266, 353, 383, 406 |
| ScrFI | CCNGG | 3 cut(s) | 70, 248, 605 |
| SduI | GDGCHC | 2 cut(s) | 931, 1066 |
| SfaNI | GCATC | 6 cut(s) | 13, 276, 339, 522, 1578, 1798 |
| SfcI | CTRYAG | 2 cut(s) | 636, 1339 |
| Sfr303I | CCGCGG | 1 cut(s) | 1096 |
| SfuI | TTCGAA | 1 cut(s) | 303 |
| SgrBI | CCGCGG | 1 cut(s) | 1096 |
| SinI | GGWCC | 1 cut(s) | 1322 |
| SmiI | ATTTAAAT | 1 cut(s) | 702 |
| SmiMI | CAYNNNNRTG | 6 cut(s) | 427, 702, 761, 1028, 1230, 1391 |
| SmlI | CTYRAG | 2 cut(s) | 1133, 1706 |
| SmoI | CTYRAG | 2 cut(s) | 1133, 1706 |
| SphI | GCATGC | 1 cut(s) | 849 |
| SseBI | AGGCCT | 1 cut(s) | 580 |
| SsiI | CCGC | 9 cut(s) | 77, 98, 220, 283, 1093, 1095, 1378, 1459, 1768 |
| SspMI | CTAG | 3 cut(s) | 1157, 1355, 1605 |
| SstI | GAGCTC | 1 cut(s) | 1066 |
| StuI | AGGCCT | 1 cut(s) | 580 |
| StyD4I | CCNGG | 3 cut(s) | 68, 246, 603 |
| StyI | CCWWGG | 2 cut(s) | 210, 743 |
| SwaI | ATTTAAAT | 1 cut(s) | 702 |
| TaaI | ACNGT | 6 cut(s) | 177, 913, 982, 999, 1518, 1743 |
| TaiI | ACGT | 3 cut(s) | 312, 369, 447 |
| TatI | WGTACW | 3 cut(s) | 713, 731, 1840 |
| TauI | GCSGC | 4 cut(s) | 80, 222, 1381, 1461 |
| TfiI | GAWTC | 3 cut(s) | 688, 748, 1448 |
| Tru1I | TTAA | 6 cut(s) | 701, 851, 1134, 1413, 1622, 1661 |
| Tru9I | TTAA | 6 cut(s) | 701, 851, 1134, 1413, 1622, 1661 |
| TscAI | CASTG | 1 cut(s) | 182 |
| TseFI | GTSAC | 1 cut(s) | 420 |
| TseI | GCWGC | 4 cut(s) | 154, 157, 470, 1747 |
| Tsp45I | GTSAC | 1 cut(s) | 420 |
| TspDTI | ATGAA | 6 cut(s) | 158, 223, 417, 740, 792, 1053 |
| TspRI | CASTG | 1 cut(s) | 182 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 177, 1180 |
| Vha464I | CTTAAG | 1 cut(s) | 1133 |
| VpaK11BI | GGWCC | 1 cut(s) | 1322 |
| XapI | RAATTY | 1 cut(s) | 1152 |
| XbaI | TCTAGA | 1 cut(s) | 1156 |
| XceI | RCATGY | 4 cut(s) | 154, 681, 760, 849 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 268 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1452 |
| XspI | CTAG | 3 cut(s) | 1157, 1355, 1605 |
| Zsp2I | ATGCAT | 2 cut(s) | 38, 1813 |