Rw2G040500

Encoded by

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000917
Physical Location & Seq
Forward (+)
65983459 .. 65985662
2204 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G040500.1

Sequence Viewer

Length: 1827 bp
ATGTCAAACTCATTGTTTTCAAGAGATCCAGACAAGTTCATGCCCAAGTTGCTGGATCATCTGAGAAAAGCCAGAGCCGTGGCCGACACAGATGACGAAGCTTTACTCGTGAGCTTCAAAAAGATGGAAGATGAGCTTGAGAACATCAAAGGCGTGCTACCCAGAGTGAAGCACTGGGAGCAAACTCTGGTGAACCAGTTCACTAACTTGGAGCGCCGCCTCGACAAGATCATCTTGGGCACTGAGACTTCCATCGAGGCCATCACCGCCACACTAAACCGCATGTTTCAAAATGTGGAGCAAATCAAACTCTCACATCCTCTAGTGCAGCTGATGCCTTCACCCGAGCGCAGCTCCGAGTCGACATGGACGCGCAGATCTCAGCCGGAGTTGAGTCAGGGAATGTCACCCGAATGGTCCGAATTGAGCCTCGAAAATTGGGTTGATAACACTCGGAGCATGGAGGATATAAAGAGAAGCTATGAACATCCTGAGAGCGTTGAGTTGAAGATGTGCTGGTATCTTATAATGGTAGGTACCATAGCTTGTCTCCTGATTTACTGGTGGATTGGCGAGGGTTTCGTTACAACCACTAAAGGCAGGACAGCTGAAGAGACAGGTGAGGAAATCTTCCGGAAACTCATAATCTCGGGTTTGATTCAAGTTCAACCACATAATGCATTTAGTTCTATAGTTGTGAATAGTTGTACAGTGCATCCTTGGGTTCGTCATATGTTGGTCTCCTTGGCCAAGCACGCTAAGCTTTTCGACTTCGATTCAAGATGGCCAAGGATGCCGTCTTATAGTACAGATAGTATTTCAAATTGTAGACGTGCATGTTTAGTTTATGACAACCCAATTTCTGAGGGGTCTAATGGCCGGAAAGTGGAGGATCTGTTGACAGTGTTCAATGTGAACGAACAGTATCTTAGTTTGAAACGGGAATGGCTTAGGAAGTTGAAAAAAGTTGAGGTGATTCAGCTAGGACGGTGGCAGAACTCGGCTTCGCATCACATTGAAGTCGAAGATAAAGGGTTTTTGAAAGGTTTAGGGGCTCAGAATAAGCTCTTGAAGTATCTTTGCCTCCGAGGGATATCAGGAATTACCAAACTCCCTTCTTCTATCTTGAAGCTTCTCAACCTTGAAATTCTTGATCTGAGGGCATGCCACAGCTTAGAGACTTTGCCTCCAGATATCTCACCATTGAGGAATCTCACGCATTTCGACATATCCGAGTGTTACTTGCTAGAAGCAATGCCGAAAGGAATTGAGAAGCTGTCTTCTCTTCAAGTGCTAAAAGGCTTTGTGATTGGGGATTTGGAAGGAACTCCCTGCAGAATTGGTGATCTTGCACAGCTGAGGCATCTAAAGCGGCTCAGTATACACATGGGCAATCAGGCTGAAGAAATTGTATCTCTTCGCCACCTCAAACTTTCATGGGGAGTTGTTTCTCCAAACTTGAAAGCAAAAGTTGCCTTCCAGTCCTTGGAGTTCTCTTTTCCGCCGGATTTAGAAAAATTGGATCTTCAAGGCATCCCTCTAGCACATGTACCAGAATGGTTGAACCCTGTCAAGCTCAAGAATTTAAAAAAGCTCTATATAAGGGGTGGGGAACTAGATAGCTTGGATCATGGAGAGACTGAGAAGTGGACTGTTGAAGTCTTGCGTCTCAAGCACTTGAATAATTTGGAGATAGGTTTGCCAAAGTTGAAGGATGAGTTTCCTCGTCTGCGCTATTTGGAGAAAATCAACTGTCATGAGATTGAAAAGGATCGATACGATGAAGACATTGTTTGGAGTGAATATGCAGAGTACACCTGCATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

608

Amino Acids

70.05

Weight (kDa)

6.58

Isoelectric Point (pI)

49.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WHD_DRP PF23559 185 - 243 7.6e-09 Disease resistance protein Winged helix domain
LRR_14 PF23598 356 - 539 1.2e-21 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 527
Acc65I GGTACC 1 cut(s) 536
AccB1I GGYRCC 1 cut(s) 536
AccB7I CCANNNNNTGG 1 cut(s) 1486
AccI GTMKAC 3 cut(s) 362, 829, 1381
AccII CGCG 1 cut(s) 373
AccIII TCCGGA 1 cut(s) 633
AciI CCGC 5 cut(s) 217, 267, 280, 1372, 1502
AclWI GGATC 6 cut(s) 20, 63, 900, 1530, 1635, 1779
AcoI YGGCCR 4 cut(s) 81, 747, 785, 877
AcsI RAATTY 2 cut(s) 1146, 1582
AcuI CTGAAG 2 cut(s) 630, 1422
AfaI GTAC 5 cut(s) 538, 709, 808, 1551, 1814
AfiI CCNNNNNNNGG 2 cut(s) 886, 1486
AflIII ACRYGT 1 cut(s) 1546
AjiI CACGTC 1 cut(s) 833
Alw26I GTCTC 7 cut(s) 239, 554, 608, 745, 1172, 1631, 1673
AlwI GGATC 6 cut(s) 20, 63, 900, 1530, 1635, 1779
Ama87I CYCGRG 2 cut(s) 344, 649
Aor13HI TCCGGA 1 cut(s) 633
AoxI GGCC 5 cut(s) 81, 258, 747, 785, 877
ApeKI GCWGC 2 cut(s) 328, 351
ApoI RAATTY 2 cut(s) 1146, 1582
Asp700I GAANNNNTTC 1 cut(s) 197
Asp718I GGTACC 1 cut(s) 536
AspLEI GCGC 4 cut(s) 216, 351, 375, 1734
AspS9I GGNCC 1 cut(s) 417
AsuHPI GGTGA 8 cut(s) 202, 256, 333, 399, 632, 985, 1191, 1355
AvaI CYCGRG 2 cut(s) 344, 649
AvaII GGWCC 1 cut(s) 417
BaeGI GKGCMC 1 cut(s) 242
BalI TGGCCA 2 cut(s) 749, 787
BanI GGYRCC 1 cut(s) 536
BanII GRGCYC 1 cut(s) 1057
BauI CACGAG 1 cut(s) 107
BbsI GAAGAC 2 cut(s) 1272, 1791
BbvCI CCTCAGC 1 cut(s) 1358
BbvI GCAGC 2 cut(s) 340, 363
BccI CCATC 4 cut(s) 118, 260, 269, 777
BceAI ACGGC 2 cut(s) 62, 781
BcoDI GTCTC 7 cut(s) 239, 554, 608, 745, 1172, 1631, 1673
BfaI CTAG 5 cut(s) 323, 983, 1247, 1541, 1616
BfmI CTRYAG 2 cut(s) 690, 1333
BfoI RGCGCY 1 cut(s) 217
BglII AGATCT 1 cut(s) 377
BisI GCNGC 4 cut(s) 217, 329, 352, 1373
BlpI GCTNAGC 1 cut(s) 759
BlsI GCNGC 4 cut(s) 218, 330, 353, 1374
Bme18I GGWCC 1 cut(s) 417
BmeT110I CYCGRG 2 cut(s) 344, 649
BmgBI CACGTC 1 cut(s) 833
BmgT120I GGNCC 1 cut(s) 417
BmiI GGNNCC 1 cut(s) 538
BmrI ACTGGG 1 cut(s) 184
BmsI GCATC 6 cut(s) 324, 724, 783, 1018, 1372, 1542
BmuI ACTGGG 1 cut(s) 184
BpiI GAAGAC 2 cut(s) 1272, 1791
BplI GAGNNNNNCTC 2 cut(s) 338, 370
BpmI CTGGAG 1 cut(s) 1173
Bpu10I CCTNAGC 2 cut(s) 950, 1358
Bpu1102I GCTNAGC 1 cut(s) 759
BpuEI CTTGAG 3 cut(s) 158, 1562, 1655
Bsa29I ATCGAT 1 cut(s) 1774
BsaI GGTCTC 1 cut(s) 745
BsaJI CCNNGG 6 cut(s) 78, 719, 744, 788, 1087, 1485
BsaWI WCCGGW 1 cut(s) 633
BsaXI ACNNNNNCTCC 4 cut(s) 881, 911, 1171, 1201
Bsc4I CCNNNNNNNGG 2 cut(s) 886, 1486
Bse1I ACTGG 4 cut(s) 179, 196, 566, 1480
Bse3DI GCAATG 1 cut(s) 1260
BseAI TCCGGA 1 cut(s) 633
BseCI ATCGAT 1 cut(s) 1774
BseDI CCNNGG 6 cut(s) 78, 719, 744, 788, 1087, 1485
BseGI GGATG 6 cut(s) 316, 487, 715, 798, 1533, 1720
BseLI CCNNNNNNNGG 2 cut(s) 886, 1486
BseMI GCAATG 1 cut(s) 1260
BseNI ACTGG 4 cut(s) 179, 196, 566, 1480
BseSI GKGCMC 1 cut(s) 242
BseXI GCAGC 2 cut(s) 340, 363
BsgI GTGCAG 1 cut(s) 347
Bsh1236I CGCG 1 cut(s) 373
BshFI GGCC 5 cut(s) 83, 260, 749, 787, 879
BshNI GGYRCC 1 cut(s) 536
BshVI ATCGAT 1 cut(s) 1774
BsiHKCI CYCGRG 2 cut(s) 344, 649
BsiSI CCGG 4 cut(s) 386, 634, 880, 1505
BslI CCNNNNNNNGG 2 cut(s) 886, 1486
BsmAI GTCTC 7 cut(s) 239, 554, 608, 745, 1172, 1631, 1673
BsmBI CGTCTC 1 cut(s) 1673
BsnI GGCC 5 cut(s) 83, 260, 749, 787, 879
Bso31I GGTCTC 1 cut(s) 745
BsoBI CYCGRG 2 cut(s) 344, 649
Bsp1286I GDGCHC 2 cut(s) 242, 1057
Bsp13I TCCGGA 1 cut(s) 633
Bsp1407I TGTACA 1 cut(s) 707
Bsp1720I GCTNAGC 1 cut(s) 759
BspACI CCGC 5 cut(s) 217, 267, 280, 1372, 1502
BspANI GGCC 5 cut(s) 83, 260, 749, 787, 879
BspDI ATCGAT 1 cut(s) 1774
BspEI TCCGGA 1 cut(s) 633
BspFNI CGCG 1 cut(s) 373
BspHI TCATGA 1 cut(s) 1756
BspLI GGNNCC 1 cut(s) 538
BspMAI CTGCAG 1 cut(s) 1337
BspPI GGATC 6 cut(s) 20, 63, 900, 1530, 1635, 1779
BspT107I GGYRCC 1 cut(s) 536
BspTNI GGTCTC 1 cut(s) 745
BsrDI GCAATG 1 cut(s) 1260
BsrGI TGTACA 1 cut(s) 707
BsrI ACTGG 4 cut(s) 179, 196, 566, 1480
BssECI CCNNGG 6 cut(s) 78, 719, 744, 788, 1087, 1485
BssNAI GTATAC 1 cut(s) 1382
BssSI CACGAG 1 cut(s) 107
BssT1I CCWWGG 4 cut(s) 719, 744, 788, 1485
Bst1107I GTATAC 1 cut(s) 1382
Bst2BI CACGAG 1 cut(s) 107
Bst4CI ACNGT 6 cut(s) 712, 904, 924, 990, 1654, 1754
Bst6I CTCTTC 3 cut(s) 606, 1290, 1422
BstAPI GCANNNNNTGC 2 cut(s) 334, 1472
BstAUI TGTACA 1 cut(s) 707
BstC8I GCNNGC 3 cut(s) 155, 756, 1165
BstDSI CCRYGG 1 cut(s) 78
BstF5I GGATG 6 cut(s) 316, 487, 715, 798, 1533, 1720
BstFNI CGCG 1 cut(s) 373
BstH2I RGCGCY 1 cut(s) 217
BstHHI GCGC 4 cut(s) 216, 351, 375, 1734
BstMAI GTCTC 7 cut(s) 239, 554, 608, 745, 1172, 1631, 1673
BstNSI RCATGY 4 cut(s) 286, 840, 1167, 1550
BstSFI CTRYAG 2 cut(s) 690, 1333
BstSLI GKGCMC 1 cut(s) 242
BstUI CGCG 1 cut(s) 373
BstV1I GCAGC 2 cut(s) 340, 363
BstV2I GAAGAC 2 cut(s) 1272, 1791
BstX2I RGATCY 4 cut(s) 25, 377, 892, 1522
BstXI CCANNNNNNTGG 2 cut(s) 52, 79
BstYI RGATCY 4 cut(s) 25, 377, 892, 1522
BstZ17I GTATAC 1 cut(s) 1382
Bsu15I ATCGAT 1 cut(s) 1774
BsuRI GGCC 5 cut(s) 83, 260, 749, 787, 879
BsuTUI ATCGAT 1 cut(s) 1774
BtgI CCRYGG 1 cut(s) 78
BtrI CACGTC 1 cut(s) 833
BtsCI GGATG 6 cut(s) 316, 487, 715, 798, 1533, 1720
BtsIMutI CAGTG 4 cut(s) 172, 240, 717, 909
Cac8I GCNNGC 3 cut(s) 155, 756, 1165
CciI TCATGA 1 cut(s) 1756
CfoI GCGC 4 cut(s) 216, 351, 375, 1734
Cfr13I GGNCC 1 cut(s) 417
ClaI ATCGAT 1 cut(s) 1774
CseI GACGC 2 cut(s) 379, 1655
Csp6I GTAC 5 cut(s) 537, 708, 807, 1550, 1813
CviQI GTAC 5 cut(s) 537, 708, 807, 1550, 1813
DraI TTTAAA 1 cut(s) 1587
EaeI YGGCCR 4 cut(s) 81, 747, 785, 877
Eam1104I CTCTTC 3 cut(s) 606, 1290, 1422
EarI CTCTTC 3 cut(s) 606, 1290, 1422
EciI GGCGGA 1 cut(s) 1491
Eco130I CCWWGG 4 cut(s) 719, 744, 788, 1485
Eco24I GRGCYC 1 cut(s) 1057
Eco31I GGTCTC 1 cut(s) 745
Eco32I GATATC 2 cut(s) 1095, 1195
Eco47I GGWCC 1 cut(s) 417
Eco57I CTGAAG 2 cut(s) 630, 1422
Eco88I CYCGRG 2 cut(s) 344, 649
EcoRV GATATC 2 cut(s) 1095, 1195
EcoT14I CCWWGG 4 cut(s) 719, 744, 788, 1485
EcoT22I ATGCAT 1 cut(s) 682
EcoT38I GRGCYC 1 cut(s) 1057
ErhI CCWWGG 4 cut(s) 719, 744, 788, 1485
Esp3I CGTCTC 1 cut(s) 1673
FalI AAGNNNNNCTT 4 cut(s) 120, 152, 218, 250
FauNDI CATATG 2 cut(s) 732, 1823
FblI GTMKAC 3 cut(s) 362, 829, 1381
Fnu4HI GCNGC 4 cut(s) 217, 329, 352, 1373
FokI GGATG 6 cut(s) 303, 474, 702, 805, 1520, 1727
FriOI GRGCYC 1 cut(s) 1057
Fsp4HI GCNGC 4 cut(s) 217, 329, 352, 1373
FspBI CTAG 5 cut(s) 323, 983, 1247, 1541, 1616
GlaI GCGC 4 cut(s) 215, 350, 374, 1733
GluI GCNGC 4 cut(s) 217, 329, 352, 1373
GsuI CTGGAG 1 cut(s) 1173
HaeII RGCGCY 1 cut(s) 217
HaeIII GGCC 5 cut(s) 83, 260, 749, 787, 879
HapII CCGG 4 cut(s) 386, 634, 880, 1505
HgaI GACGC 2 cut(s) 379, 1655
HhaI GCGC 4 cut(s) 216, 351, 375, 1734
Hin6I GCGC 4 cut(s) 214, 349, 373, 1732
HinP1I GCGC 4 cut(s) 214, 349, 373, 1732
HincII GTYRAC 2 cut(s) 363, 900
HindII GTYRAC 2 cut(s) 363, 900
HindIII AAGCTT 3 cut(s) 99, 761, 1130
HinfI GANTC 6 cut(s) 359, 394, 658, 776, 976, 1210
HpaII CCGG 4 cut(s) 386, 634, 880, 1505
HphI GGTGA 8 cut(s) 202, 256, 333, 399, 632, 985, 1191, 1355
Hpy166II GTNNAC 9 cut(s) 193, 201, 363, 830, 900, 916, 1382, 1650, 1815
Hpy188I TCNGA 9 cut(s) 63, 358, 421, 456, 865, 1059, 1088, 1158, 1234
Hpy8I GTNNAC 9 cut(s) 193, 201, 363, 830, 900, 916, 1382, 1650, 1815
HpyAV CCTTC 5 cut(s) 348, 1125, 1316, 1486, 1705
HpyCH4III ACNGT 6 cut(s) 712, 904, 924, 990, 1654, 1754
HpyCH4IV ACGT 1 cut(s) 832
HpyCH4V TGCA 8 cut(s) 328, 680, 715, 836, 1335, 1352, 1808, 1821
HpySE526I ACGT 1 cut(s) 832
HspAI GCGC 4 cut(s) 214, 349, 373, 1732
Kpn2I TCCGGA 1 cut(s) 633
KpnI GGTACC 1 cut(s) 540
LmnI GCTCC 5 cut(s) 178, 211, 298, 359, 456
Lsp1109I GCAGC 2 cut(s) 340, 363
LweI GCATC 6 cut(s) 324, 724, 783, 1018, 1372, 1542
MaeI CTAG 5 cut(s) 323, 983, 1247, 1541, 1616
MaeII ACGT 1 cut(s) 832
MaeIII GTNAC 3 cut(s) 405, 583, 1238
MflI RGATCY 4 cut(s) 25, 377, 892, 1522
MhlI GDGCHC 2 cut(s) 242, 1057
MlsI TGGCCA 2 cut(s) 749, 787
MluNI TGGCCA 2 cut(s) 749, 787
MlyI GAGTC 2 cut(s) 368, 403
Mox20I TGGCCA 2 cut(s) 749, 787
Mph1103I ATGCAT 1 cut(s) 682
MroI TCCGGA 1 cut(s) 633
MroXI GAANNNNTTC 1 cut(s) 197
MscI TGGCCA 2 cut(s) 749, 787
MseI TTAA 1 cut(s) 1586
MslI CAYNNNNRTG 1 cut(s) 412
Msp20I TGGCCA 2 cut(s) 749, 787
MspA1I CMGCKG 3 cut(s) 331, 608, 1357
MspI CCGG 4 cut(s) 386, 634, 880, 1505
MvnI CGCG 1 cut(s) 373
NdeI CATATG 2 cut(s) 732, 1823
NlaIV GGNNCC 1 cut(s) 538
NmeAIII GCCGAG 1 cut(s) 980
NmuCI GTSAC 1 cut(s) 405
NsiI ATGCAT 1 cut(s) 682
NspI RCATGY 4 cut(s) 286, 840, 1167, 1550
PaeI GCATGC 1 cut(s) 1167
PagI TCATGA 1 cut(s) 1756
PciI ACATGT 1 cut(s) 1546
PcsI WCGNNNNNNNCGW 1 cut(s) 1230
PdmI GAANNNNTTC 1 cut(s) 197
PfeI GAWTC 4 cut(s) 658, 776, 976, 1210
PflMI CCANNNNNTGG 1 cut(s) 1486
PkrI GCNGC 4 cut(s) 218, 330, 353, 1374
PleI GAGTC 2 cut(s) 367, 402
PpsI GAGTC 2 cut(s) 367, 402
PscI ACATGT 1 cut(s) 1546
PsiI TTATAA 1 cut(s) 527
PspN4I GGNNCC 1 cut(s) 538
PspPI GGNCC 1 cut(s) 417
PstI CTGCAG 1 cut(s) 1337
PsuI RGATCY 4 cut(s) 25, 377, 892, 1522
PvuII CAGCTG 3 cut(s) 331, 608, 1357
RsaI GTAC 5 cut(s) 538, 709, 808, 1551, 1814
RsaNI GTAC 5 cut(s) 537, 708, 807, 1550, 1813
RseI CAYNNNNRTG 1 cut(s) 412
SalI GTCGAC 1 cut(s) 361
SaqAI TTAA 1 cut(s) 1586
SatI GCNGC 4 cut(s) 217, 329, 352, 1373
Sau96I GGNCC 1 cut(s) 417
SchI GAGTC 2 cut(s) 368, 403
SduI GDGCHC 2 cut(s) 242, 1057
SfaNI GCATC 6 cut(s) 324, 724, 783, 1018, 1372, 1542
SfcI CTRYAG 2 cut(s) 690, 1333
SinI GGWCC 1 cut(s) 417
SmiMI CAYNNNNRTG 1 cut(s) 412
SmlI CTYRAG 3 cut(s) 137, 1577, 1670
SmoI CTYRAG 3 cut(s) 137, 1577, 1670
SphI GCATGC 1 cut(s) 1167
SsiI CCGC 5 cut(s) 217, 267, 280, 1372, 1502
SspMI CTAG 5 cut(s) 323, 983, 1247, 1541, 1616
StyI CCWWGG 4 cut(s) 719, 744, 788, 1485
TaaI ACNGT 6 cut(s) 712, 904, 924, 990, 1654, 1754
TaiI ACGT 1 cut(s) 835
TaqI TCGA 9 cut(s) 222, 255, 362, 432, 768, 774, 1023, 1224, 1774
TatI WGTACW 3 cut(s) 707, 806, 1812
TauI GCSGC 2 cut(s) 219, 1375
TfiI GAWTC 4 cut(s) 658, 776, 976, 1210
Tru1I TTAA 1 cut(s) 1586
Tru9I TTAA 1 cut(s) 1586
TscAI CASTG 4 cut(s) 179, 247, 717, 909
TseFI GTSAC 1 cut(s) 405
TseI GCWGC 2 cut(s) 328, 351
Tsp45I GTSAC 1 cut(s) 405
TspDTI ATGAA 4 cut(s) 28, 498, 1425, 1797
TspRI CASTG 4 cut(s) 179, 247, 717, 909
Van91I CCANNNNNTGG 1 cut(s) 1486
VpaK11BI GGWCC 1 cut(s) 417
XapI RAATTY 2 cut(s) 1146, 1582
XceI RCATGY 4 cut(s) 286, 840, 1167, 1550
XmiI GTMKAC 3 cut(s) 362, 829, 1381
XmnI GAANNNNTTC 1 cut(s) 197
XspI CTAG 5 cut(s) 323, 983, 1247, 1541, 1616
Zsp2I ATGCAT 1 cut(s) 682
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.