Rorug02G0429200

Domain of unknown function (DUF4220)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0000662
Physical Location & Seq
Reverse (-)
54963804 .. 54968816
5013 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0429200.1

Sequence Viewer

Length: 1041 bp
ATGATTCAATTTCTGGGCACCCTTCGCCCTTCTCCTTCTTTAAGCTCTTTCGCTACTTACAAACGCCAAGTCTACTCGCCAATCACAGCTCCCAGAAATGGGTTCTTGTCCTTCAGACGAATGTTCAAGGCTTACATTCAATCCAGTTCAGCAGCCACGGATGCTGGTTTGGGGTTACCATCTGAGCAATCAGAAGTCATATTTATAGGTACAGGAACGAGCGAAGGAATTCCACGTGTGAGCTGCCTGACAAATCCTTTGAAGACATGTGAGGTTTGTTTAAAAGCTGTGGAACCGGGTAATAAAAATAGGAGACTCAACACAAGCATTGTTGTACGTCATCCTACATCCTCTGGAAGTTCTAACATTCTTATTGATGCTGGAAAATTTTTCTATCACAGTGCTCTCAGATGGTTTCCTGCTTATGGGATAAGAACACTTGATGCGGTTATTATTACTCATTCTCATGCTGATGCAATTGGAGGTCTGGATGATCTTCGTGATTGGACGAACAATGTTCAATCCAGCATTCCAATATATGTGCCTGAGCGTGATTTTGAGGTGATGAAGAAGACTCATTATTATTTGGTTGACACAAGTGTGGTCCAACCTGGTGCTGCTGTCTCAGAGTTGCAATTTAATATTATACATGAGGATCCATTTTCTGTGAGCAATTTGAAGTTTACCCCTTTACCTGTATGGCATGGTCGTGGTTATCGTTCCTTTGGATATCGTTTTGGTAATGTTTGTTATATCAGTGACGTTAGCGAAATACCTGAAGAAACCTACCCACTTCTGAGAGATTGTGAACTCCTGATTATGGATGCTCTACGGCCAGATCGGAATTCTGCAACACATTTTGCACTTCCAAGGGCTTTGGAGGAAGTACGGAAAATACAACCTAAGAGAACATTGTTTACTGGTATGATGCATTTGATGGATCATGAGAAGGTGAATGATTATCTTATGAAATTGAAGGAGACAGAGGGTCTTGATGCACAGTTGAGCTATGATGGGCTTCGGGTACCTATAGCACTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4220 DUF594

Protein Analysis

346

Amino Acids

38.89

Weight (kDa)

7.69

Isoelectric Point (pI)

51.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lactamase_B PF00753 108 - 259 4.4e-09 Metallo-beta-lactamase superfamily
Anti-Pycsar_Apyc1 PF23023 119 - 289 8.1e-08 Anti-Pycsar protein Apyc1
Lactamase_B_2 PF12706 122 - 308 8.3e-26 Beta-lactamase superfamily domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1024
AccB1I GGYRCC 2 cut(s) 17, 1024
AccI GTMKAC 1 cut(s) 72
AciI CCGC 1 cut(s) 446
AclWI GGATC 3 cut(s) 650, 663, 948
AcoI YGGCCR 1 cut(s) 833
AcsI RAATTY 3 cut(s) 228, 386, 844
AcuI CTGAAG 2 cut(s) 97, 798
AcvI CACGTG 1 cut(s) 236
AfaI GTAC 4 cut(s) 211, 336, 888, 1026
AfiI CCNNNNNNNGG 4 cut(s) 98, 99, 425, 820
AflIII ACRYGT 2 cut(s) 235, 266
AgsI TTSAA 7 cut(s) 8, 127, 140, 262, 521, 679, 976
AhdI GACNNNNNGTC 1 cut(s) 987
AjnI CCWGG 1 cut(s) 610
AleI CACNNNNGTG 1 cut(s) 599
AluBI AGCT 5 cut(s) 45, 89, 243, 287, 1008
AluI AGCT 5 cut(s) 45, 89, 243, 287, 1008
Alw21I GWGCWC 1 cut(s) 406
Alw26I GTCTC 3 cut(s) 307, 628, 974
AlwI GGATC 3 cut(s) 650, 663, 948
AoxI GGCC 1 cut(s) 833
ApeKI GCWGC 3 cut(s) 152, 243, 617
ApoI RAATTY 3 cut(s) 228, 386, 844
ArsI GACNNNNNNTTYG 4 cut(s) 54, 86, 241, 273
Asp700I GAANNNNTTC 1 cut(s) 228
Asp718I GGTACC 1 cut(s) 1024
AspS9I GGNCC 1 cut(s) 604
AsuC2I CCSGG 1 cut(s) 297
AsuHPI GGTGA 2 cut(s) 574, 964
AvaII GGWCC 1 cut(s) 604
BaeGI GKGCMC 1 cut(s) 20
BamHI GGATCC 1 cut(s) 655
BanI GGYRCC 2 cut(s) 17, 1024
BbrPI CACGTG 1 cut(s) 236
BbsI GAAGAC 2 cut(s) 269, 578
Bbv12I GWGCWC 1 cut(s) 406
BbvI GCAGC 3 cut(s) 164, 230, 604
BccI CCATC 4 cut(s) 187, 405, 931, 1007
BceAI ACGGC 1 cut(s) 848
BciT130I CCWGG 1 cut(s) 612
BcnI CCSGG 1 cut(s) 297
BcoDI GTCTC 3 cut(s) 307, 628, 974
BfmI CTRYAG 1 cut(s) 1029
BisI GCNGC 3 cut(s) 153, 244, 618
BlsI GCNGC 3 cut(s) 154, 245, 619
Bme1390I CCNGG 2 cut(s) 297, 612
Bme18I GGWCC 1 cut(s) 604
BmeRI GACNNNNNGTC 1 cut(s) 987
BmgT120I GGNCC 1 cut(s) 604
BmiI GGNNCC 4 cut(s) 19, 294, 657, 1026
BmrFI CCNGG 2 cut(s) 297, 612
BmsI GCATC 7 cut(s) 151, 367, 433, 463, 814, 918, 985
BpiI GAAGAC 2 cut(s) 269, 578
Bpu10I CCTNAGC 1 cut(s) 546
BpuMI CCSGG 1 cut(s) 297
BsaAI YACGTR 1 cut(s) 236
BsaJI CCNNGG 2 cut(s) 156, 869
Bsc4I CCNNNNNNNGG 4 cut(s) 98, 99, 425, 820
Bse1I ACTGG 2 cut(s) 144, 925
BseBI CCWGG 1 cut(s) 612
BseDI CCNNGG 2 cut(s) 156, 869
BseGI GGATG 5 cut(s) 166, 340, 347, 496, 829
BseLI CCNNNNNNNGG 4 cut(s) 98, 99, 425, 820
BseMII CTCAG 5 cut(s) 174, 421, 537, 639, 788
BseNI ACTGG 2 cut(s) 144, 925
BseSI GKGCMC 1 cut(s) 20
BseXI GCAGC 3 cut(s) 164, 230, 604
BshFI GGCC 1 cut(s) 835
BshNI GGYRCC 2 cut(s) 17, 1024
BsiHKAI GWGCWC 1 cut(s) 406
BsiSI CCGG 1 cut(s) 296
BslI CCNNNNNNNGG 4 cut(s) 98, 99, 425, 820
BsmAI GTCTC 3 cut(s) 307, 628, 974
BsmI GAATGC 1 cut(s) 528
BsnI GGCC 1 cut(s) 835
Bsp1286I GDGCHC 2 cut(s) 20, 406
Bsp143I GATC 4 cut(s) 493, 655, 838, 940
BspACI CCGC 1 cut(s) 446
BspANI GGCC 1 cut(s) 835
BspCNI CTCAG 5 cut(s) 175, 420, 538, 638, 789
BspHI TCATGA 1 cut(s) 943
BspLI GGNNCC 4 cut(s) 19, 294, 657, 1026
BspPI GGATC 3 cut(s) 650, 663, 948
BspT107I GGYRCC 2 cut(s) 17, 1024
BsrI ACTGG 2 cut(s) 144, 925
BssECI CCNNGG 2 cut(s) 156, 869
BssMI GATC 4 cut(s) 493, 655, 838, 940
BssT1I CCWWGG 1 cut(s) 869
Bst2UI CCWGG 1 cut(s) 612
Bst4CI ACNGT 2 cut(s) 401, 1002
BstBAI YACGTR 1 cut(s) 236
BstDEI CTNAG 6 cut(s) 183, 407, 546, 625, 797, 903
BstDSI CCRYGG 1 cut(s) 156
BstEII GGTNACC 1 cut(s) 174
BstF5I GGATG 5 cut(s) 166, 340, 347, 496, 829
BstKTI GATC 4 cut(s) 496, 658, 841, 943
BstMAI GTCTC 3 cut(s) 307, 628, 974
BstMBI GATC 4 cut(s) 493, 655, 838, 940
BstMWI GCNNNNNNNGC 2 cut(s) 24, 161
BstNI CCWGG 1 cut(s) 612
BstNSI RCATGY 1 cut(s) 270
BstPI GGTNACC 1 cut(s) 174
BstSCI CCNGG 2 cut(s) 295, 610
BstSFI CTRYAG 1 cut(s) 1029
BstSLI GKGCMC 1 cut(s) 20
BstV1I GCAGC 3 cut(s) 164, 230, 604
BstV2I GAAGAC 2 cut(s) 269, 578
BstX2I RGATCY 1 cut(s) 655
BstYI RGATCY 1 cut(s) 655
BsuRI GGCC 1 cut(s) 835
BtgI CCRYGG 1 cut(s) 156
BtsCI GGATG 5 cut(s) 166, 340, 347, 496, 829
BtsIMutI CAGTG 2 cut(s) 406, 763
CciI TCATGA 1 cut(s) 943
Cfr13I GGNCC 1 cut(s) 604
CsiI ACCWGGT 1 cut(s) 610
Csp6I GTAC 4 cut(s) 210, 335, 887, 1025
CviAII CATG 5 cut(s) 267, 467, 650, 704, 944
CviQI GTAC 4 cut(s) 210, 335, 887, 1025
DdeI CTNAG 6 cut(s) 183, 407, 546, 625, 797, 903
DpnI GATC 4 cut(s) 495, 657, 840, 942
DpnII GATC 4 cut(s) 493, 655, 838, 940
DraI TTTAAA 1 cut(s) 282
DriI GACNNNNNGTC 1 cut(s) 987
EaeI YGGCCR 1 cut(s) 833
Eam1105I GACNNNNNGTC 1 cut(s) 987
Eco130I CCWWGG 1 cut(s) 869
Eco32I GATATC 1 cut(s) 731
Eco47I GGWCC 1 cut(s) 604
Eco57I CTGAAG 2 cut(s) 97, 798
Eco72I CACGTG 1 cut(s) 236
Eco91I GGTNACC 1 cut(s) 174
EcoO65I GGTNACC 1 cut(s) 174
EcoRI GAATTC 2 cut(s) 228, 844
EcoRII CCWGG 1 cut(s) 610
EcoRV GATATC 1 cut(s) 731
EcoT14I CCWWGG 1 cut(s) 869
EcoT22I ATGCAT 1 cut(s) 933
ErhI CCWWGG 1 cut(s) 869
FaeI CATG 5 cut(s) 270, 470, 653, 707, 947
FatI CATG 5 cut(s) 266, 466, 649, 703, 943
FblI GTMKAC 1 cut(s) 72
Fnu4HI GCNGC 3 cut(s) 153, 244, 618
FokI GGATG 5 cut(s) 173, 327, 334, 503, 836
Fsp4HI GCNGC 3 cut(s) 153, 244, 618
GluI GCNGC 3 cut(s) 153, 244, 618
HaeIII GGCC 1 cut(s) 835
HapII CCGG 1 cut(s) 296
Hin1II CATG 5 cut(s) 270, 470, 653, 707, 947
HincII GTYRAC 1 cut(s) 592
HindII GTYRAC 1 cut(s) 592
HinfI GANTC 3 cut(s) 4, 315, 574
HpaII CCGG 1 cut(s) 296
HphI GGTGA 2 cut(s) 574, 964
Hpy166II GTNNAC 5 cut(s) 73, 592, 684, 809, 918
Hpy188I TCNGA 7 cut(s) 116, 184, 193, 410, 628, 798, 843
Hpy188III TCNNGA 6 cut(s) 354, 488, 500, 814, 944, 992
Hpy8I GTNNAC 5 cut(s) 73, 592, 684, 809, 918
HpyAV CCTTC 7 cut(s) 32, 39, 45, 121, 218, 943, 970
HpyCH4III ACNGT 2 cut(s) 401, 1002
HpyCH4IV ACGT 3 cut(s) 235, 337, 762
HpyCH4V TGCA 6 cut(s) 476, 634, 851, 863, 931, 998
HpyF10VI GCNNNNNNNGC 2 cut(s) 24, 161
HpyF3I CTNAG 6 cut(s) 183, 407, 546, 625, 797, 903
HpySE526I ACGT 3 cut(s) 235, 337, 762
Hsp92II CATG 5 cut(s) 270, 470, 653, 707, 947
KpnI GGTACC 1 cut(s) 1028
Kzo9I GATC 4 cut(s) 493, 655, 838, 940
LmnI GCTCC 1 cut(s) 94
Lsp1109I GCAGC 3 cut(s) 164, 230, 604
LweI GCATC 7 cut(s) 151, 367, 433, 463, 814, 918, 985
MabI ACCWGGT 1 cut(s) 610
MaeII ACGT 3 cut(s) 235, 337, 762
MaeIII GTNAC 2 cut(s) 174, 758
MalI GATC 4 cut(s) 495, 657, 840, 942
MboI GATC 4 cut(s) 493, 655, 838, 940
MboII GAAGA 5 cut(s) 274, 488, 580, 583, 791
MfeI CAATTG 1 cut(s) 477
MflI RGATCY 1 cut(s) 655
MhlI GDGCHC 2 cut(s) 20, 406
MluCI AATT 8 cut(s) 8, 228, 386, 477, 635, 673, 844, 971
MlyI GAGTC 2 cut(s) 309, 568
MmeI TCCRAC 1 cut(s) 631
MnlI CCTC 7 cut(s) 265, 361, 476, 553, 646, 874, 979
Mph1103I ATGCAT 1 cut(s) 933
MroXI GAANNNNTTC 1 cut(s) 228
MseI TTAA 3 cut(s) 41, 281, 639
MslI CAYNNNNRTG 4 cut(s) 465, 471, 599, 708
MspI CCGG 1 cut(s) 296
MspR9I CCNGG 2 cut(s) 297, 612
MunI CAATTG 1 cut(s) 477
Mva1269I GAATGC 1 cut(s) 528
MvaI CCWGG 1 cut(s) 612
MwoI GCNNNNNNNGC 2 cut(s) 24, 161
NciI CCSGG 1 cut(s) 297
NdeII GATC 4 cut(s) 493, 655, 838, 940
NlaIII CATG 5 cut(s) 270, 470, 653, 707, 947
NlaIV GGNNCC 4 cut(s) 19, 294, 657, 1026
NmuCI GTSAC 1 cut(s) 758
NsiI ATGCAT 1 cut(s) 933
NspI RCATGY 1 cut(s) 270
OliI CACNNNNGTG 1 cut(s) 599
PagI TCATGA 1 cut(s) 943
PciI ACATGT 1 cut(s) 266
PcsI WCGNNNNNNNCGW 1 cut(s) 715
PctI GAATGC 1 cut(s) 528
PdmI GAANNNNTTC 1 cut(s) 228
PfeI GAWTC 1 cut(s) 4
PkrI GCNGC 3 cut(s) 154, 245, 619
PleI GAGTC 2 cut(s) 309, 568
PmaCI CACGTG 1 cut(s) 236
PmlI CACGTG 1 cut(s) 236
PpsI GAGTC 2 cut(s) 309, 568
Ppu21I YACGTR 1 cut(s) 236
PscI ACATGT 1 cut(s) 266
Psp6I CCWGG 1 cut(s) 610
PspCI CACGTG 1 cut(s) 236
PspEI GGTNACC 1 cut(s) 174
PspGI CCWGG 1 cut(s) 610
PspN4I GGNNCC 4 cut(s) 19, 294, 657, 1026
PspPI GGNCC 1 cut(s) 604
PsrI GAACNNNNNNTAC 2 cut(s) 901, 933
PsuI RGATCY 1 cut(s) 655
RsaI GTAC 4 cut(s) 211, 336, 888, 1026
RsaNI GTAC 4 cut(s) 210, 335, 887, 1025
RseI CAYNNNNRTG 4 cut(s) 465, 471, 599, 708
SaqAI TTAA 3 cut(s) 41, 281, 639
SatI GCNGC 3 cut(s) 153, 244, 618
Sau3AI GATC 4 cut(s) 493, 655, 838, 940
Sau96I GGNCC 1 cut(s) 604
SchI GAGTC 2 cut(s) 309, 568
ScrFI CCNGG 2 cut(s) 297, 612
SduI GDGCHC 2 cut(s) 20, 406
SexAI ACCWGGT 1 cut(s) 610
SfaNI GCATC 7 cut(s) 151, 367, 433, 463, 814, 918, 985
SfcI CTRYAG 1 cut(s) 1029
SinI GGWCC 1 cut(s) 604
SmiMI CAYNNNNRTG 4 cut(s) 465, 471, 599, 708
Sse9I AATT 8 cut(s) 8, 228, 386, 477, 635, 673, 844, 971
SsiI CCGC 1 cut(s) 446
SspI AATATT 1 cut(s) 643
StyD4I CCNGG 2 cut(s) 295, 610
StyI CCWWGG 1 cut(s) 869
TaaI ACNGT 2 cut(s) 401, 1002
TaiI ACGT 3 cut(s) 238, 340, 765
TasI AATT 8 cut(s) 8, 228, 386, 477, 635, 673, 844, 971
TfiI GAWTC 1 cut(s) 4
Tru1I TTAA 3 cut(s) 41, 281, 639
Tru9I TTAA 3 cut(s) 41, 281, 639
TscAI CASTG 2 cut(s) 406, 763
TseFI GTSAC 1 cut(s) 758
TseI GCWGC 3 cut(s) 152, 243, 617
Tsp45I GTSAC 1 cut(s) 758
TspDTI ATGAA 2 cut(s) 581, 983
TspGWI ACGGA 2 cut(s) 173, 904
TspRI CASTG 2 cut(s) 406, 763
VpaK11BI GGWCC 1 cut(s) 604
XapI RAATTY 3 cut(s) 228, 386, 844
XceI RCATGY 1 cut(s) 270
XmiI GTMKAC 1 cut(s) 72
XmnI GAANNNNTTC 1 cut(s) 228
Zsp2I ATGCAT 1 cut(s) 933
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.