Rorug02G0183100

Domain of unknown function (DUF4220)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0000662
Physical Location & Seq
Reverse (-)
17062964 .. 17068214
5251 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0183100.1

Sequence Viewer

Length: 960 bp
ATGGGCAACTCGTCCAGGGACGACTCCGCCACCGACGGCGACGCCTCTAGCGGCGGCGACGCCAAGCCCTCCAATTCCAGCCTCTACTCCGAGGGCGAGAAGATCCTCGCCTACCACGGCCCTCGCATCTACGAAGCCAAGGTTCAGAAGGCAGAGATCAGGAAAAACGAATGGAGATACTTTGTTCACTACCTTGGCTGGAATAAAAATTGGGACGAGTGGGTAGGTGTGGATCGCATATTGAAGTACACTGAAGAGAATATATTGAAGCAGCAAGCTCTTGACAAGAAACAGGGCGTAGACAAGAATACAAAGTCAGGACGTTCCGCTCACACGAAGCCAAAAAGTTCTGCTGATGCAAAAGTGGAGAAAGAAGACCAAAAGAACAATGTGGCCAAAGGGAAGAAGCGAAAGAATGACTCAGGGGCTGAGAAGGACAGCGTTACCTTGGAAAAGCTTGTGAAGATTCAAATTCCTTTAGCCTTAAGAAAACAACTGGTAGAGGATTGGGAATTTGTGTGTGAGCAGGATAAGCTTGTTAAACTTCCACGATCTCCAACTGTAGATGACATTTTGCAGAAGTACCTCGACTACAGGACAAAGAAAGATGGAAAGCTGACTGATCCAGTAGGGGAAGTCCTGAAAGGAATACGATGTTACTTTGACAAAGCATTGCCAATGATTCTCTTATACAAAAAGGAACGCAAACAGTATCAAGATACCATTGTAGATGATGTCTCTCCGTCAACAATATATGGTGCCGAACATCTGCTACGACTTTTTGTTAAGCTGCCGGAGCTATTGGCGTATGTCAACATTGAGGAGGAAACACTGACCCGCTTACAGCAGCAATTACTTGATTTTCTCAAATTTCTGCAGAAAAATCAGAGCACTTTCTTCCTTTCTGCTTATGATGGCTCAAAAAGTGTTGATGGGAAAGGCAAAGGAAAACATGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4220 DUF594

Protein Analysis

319

Amino Acids

36.5

Weight (kDa)

8.98

Isoelectric Point (pI)

36.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MSL3_chromo-like PF22732 29 - 95 3.4e-15 MSL3 chromodomain-like
Tudor-knot PF11717 31 - 81 1.8e-12 RNA binding activity-knot of a chromodomain
MRG PF05712 135 - 304 4.8e-50 MRG
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 758
AccBSI CCGCTC 1 cut(s) 329
AccI GTMKAC 1 cut(s) 300
AciI CCGC 5 cut(s) 27, 51, 54, 327, 838
AclWI GGATC 3 cut(s) 97, 240, 617
AcoI YGGCCR 1 cut(s) 393
AcsI RAATTY 3 cut(s) 471, 512, 869
AcuI CTGAAG 1 cut(s) 273
AcyI GRCGYC 2 cut(s) 42, 60
AfaI GTAC 2 cut(s) 248, 584
AflII CTTAAG 1 cut(s) 484
AgsI TTSAA 3 cut(s) 244, 268, 470
AjnI CCWGG 1 cut(s) 14
AluBI AGCT 6 cut(s) 278, 457, 535, 616, 790, 799
AluI AGCT 6 cut(s) 278, 457, 535, 616, 790, 799
Alw21I GWGCWC 1 cut(s) 893
Alw26I GTCTC 1 cut(s) 742
AlwI GGATC 3 cut(s) 97, 240, 617
AlwNI CAGNNNCTG 1 cut(s) 428
AoxI GGCC 2 cut(s) 118, 393
ApeKI GCWGC 3 cut(s) 271, 790, 847
ApoI RAATTY 3 cut(s) 471, 512, 869
AspS9I GGNCC 1 cut(s) 119
BaeI ACNNNNGTAYC 2 cut(s) 711, 744
BalI TGGCCA 1 cut(s) 395
BanI GGYRCC 1 cut(s) 758
BbsI GAAGAC 1 cut(s) 381
Bbv12I GWGCWC 1 cut(s) 893
BbvI GCAGC 3 cut(s) 283, 777, 859
BccI CCATC 3 cut(s) 602, 908, 926
BceAI ACGGC 2 cut(s) 52, 133
BciT130I CCWGG 1 cut(s) 16
BcoDI GTCTC 1 cut(s) 742
BfaI CTAG 1 cut(s) 48
BfmI CTRYAG 3 cut(s) 561, 592, 875
BfrI CTTAAG 1 cut(s) 484
BisI GCNGC 5 cut(s) 52, 55, 272, 791, 848
BlsI GCNGC 5 cut(s) 53, 56, 273, 792, 849
Bme1390I CCNGG 1 cut(s) 16
BmgT120I GGNCC 1 cut(s) 119
BmiI GGNNCC 1 cut(s) 760
BmrFI CCNGG 1 cut(s) 16
BmsI GCATC 2 cut(s) 135, 346
BpiI GAAGAC 1 cut(s) 381
BsaHI GRCGYC 2 cut(s) 42, 60
BsaJI CCNNGG 6 cut(s) 15, 90, 115, 138, 193, 447
Bse1I ACTGG 2 cut(s) 501, 626
Bse3DI GCAATG 1 cut(s) 671
BseBI CCWGG 1 cut(s) 16
BseDI CCNNGG 6 cut(s) 15, 90, 115, 138, 193, 447
BseMI GCAATG 1 cut(s) 671
BseMII CTCAG 2 cut(s) 420, 435
BseNI ACTGG 2 cut(s) 501, 626
BseRI GAGGAG 1 cut(s) 836
BseXI GCAGC 3 cut(s) 283, 777, 859
BshFI GGCC 2 cut(s) 120, 395
BshNI GGYRCC 1 cut(s) 758
BsiHKAI GWGCWC 1 cut(s) 893
BsiSI CCGG 1 cut(s) 794
BslFI GGGAC 2 cut(s) 32, 227
BsmAI GTCTC 1 cut(s) 742
BsmFI GGGAC 2 cut(s) 32, 227
BsnI GGCC 2 cut(s) 120, 395
Bsp1286I GDGCHC 1 cut(s) 893
Bsp143I GATC 5 cut(s) 102, 156, 232, 551, 622
BspACI CCGC 5 cut(s) 27, 51, 54, 327, 838
BspANI GGCC 2 cut(s) 120, 395
BspCNI CTCAG 2 cut(s) 421, 434
BspLI GGNNCC 1 cut(s) 760
BspMAI CTGCAG 1 cut(s) 879
BspPI GGATC 3 cut(s) 97, 240, 617
BspT107I GGYRCC 1 cut(s) 758
BspTI CTTAAG 1 cut(s) 484
BsrBI CCGCTC 1 cut(s) 329
BsrDI GCAATG 1 cut(s) 671
BsrI ACTGG 2 cut(s) 501, 626
BssECI CCNNGG 6 cut(s) 15, 90, 115, 138, 193, 447
BssMI GATC 5 cut(s) 102, 156, 232, 551, 622
BssNI GRCGYC 2 cut(s) 42, 60
BssT1I CCWWGG 3 cut(s) 138, 193, 447
Bst2UI CCWGG 1 cut(s) 16
Bst4CI ACNGT 2 cut(s) 562, 711
Bst6I CTCTTC 1 cut(s) 249
BstACI GRCGYC 2 cut(s) 42, 60
BstAFI CTTAAG 1 cut(s) 484
BstC8I GCNNGC 1 cut(s) 276
BstDEI CTNAG 2 cut(s) 421, 429
BstDSI CCRYGG 1 cut(s) 115
BstKTI GATC 5 cut(s) 105, 159, 235, 554, 625
BstMAI GTCTC 1 cut(s) 742
BstMBI GATC 5 cut(s) 102, 156, 232, 551, 622
BstMWI GCNNNNNNNGC 2 cut(s) 532, 796
BstNI CCWGG 1 cut(s) 16
BstSCI CCNGG 1 cut(s) 14
BstSFI CTRYAG 3 cut(s) 561, 592, 875
BstV1I GCAGC 3 cut(s) 283, 777, 859
BstV2I GAAGAC 1 cut(s) 381
BstX2I RGATCY 1 cut(s) 102
BstYI RGATCY 1 cut(s) 102
BsuRI GGCC 2 cut(s) 120, 395
BtgI CCRYGG 1 cut(s) 115
BtsIMutI CAGTG 2 cut(s) 249, 830
Cac8I GCNNGC 1 cut(s) 276
CaiI CAGNNNCTG 1 cut(s) 428
Cfr13I GGNCC 1 cut(s) 119
CseI GACGC 2 cut(s) 50, 68
Csp6I GTAC 2 cut(s) 247, 583
CviAII CATG 1 cut(s) 953
CviQI GTAC 2 cut(s) 247, 583
DdeI CTNAG 2 cut(s) 421, 429
DpnI GATC 5 cut(s) 104, 158, 234, 553, 624
DpnII GATC 5 cut(s) 102, 156, 232, 551, 622
EaeI YGGCCR 1 cut(s) 393
Eam1104I CTCTTC 1 cut(s) 249
EarI CTCTTC 1 cut(s) 249
EciI GGCGGA 1 cut(s) 16
Eco130I CCWWGG 3 cut(s) 138, 193, 447
Eco57I CTGAAG 1 cut(s) 273
EcoRII CCWGG 1 cut(s) 14
EcoT14I CCWWGG 3 cut(s) 138, 193, 447
ErhI CCWWGG 3 cut(s) 138, 193, 447
FaeI CATG 1 cut(s) 956
FaiI YATR 8 cut(s) 239, 263, 691, 754, 756, 810, 912, 954
FaqI GGGAC 2 cut(s) 32, 227
FatI CATG 1 cut(s) 952
FauI CCCGC 1 cut(s) 845
FblI GTMKAC 1 cut(s) 300
Fnu4HI GCNGC 5 cut(s) 52, 55, 272, 791, 848
Fsp4HI GCNGC 5 cut(s) 52, 55, 272, 791, 848
FspBI CTAG 1 cut(s) 48
GluI GCNGC 5 cut(s) 52, 55, 272, 791, 848
HaeIII GGCC 2 cut(s) 120, 395
HapII CCGG 1 cut(s) 794
HgaI GACGC 2 cut(s) 50, 68
Hin1I GRCGYC 2 cut(s) 42, 60
Hin1II CATG 1 cut(s) 956
HincII GTYRAC 2 cut(s) 747, 814
HindII GTYRAC 2 cut(s) 747, 814
HindIII AAGCTT 2 cut(s) 455, 533
HinfI GANTC 4 cut(s) 23, 419, 466, 682
HpaII CCGG 1 cut(s) 794
Hpy166II GTNNAC 5 cut(s) 187, 249, 301, 747, 814
Hpy188I TCNGA 3 cut(s) 91, 147, 888
Hpy188III TCNNGA 5 cut(s) 160, 281, 318, 640, 716
Hpy8I GTNNAC 5 cut(s) 187, 249, 301, 747, 814
Hpy99I CGWCG 3 cut(s) 38, 44, 62
HpyAV CCTTC 2 cut(s) 142, 427
HpyCH4III ACNGT 2 cut(s) 562, 711
HpyCH4IV ACGT 1 cut(s) 322
HpyCH4V TGCA 3 cut(s) 359, 577, 877
HpyF10VI GCNNNNNNNGC 2 cut(s) 532, 796
HpyF3I CTNAG 2 cut(s) 421, 429
HpySE526I ACGT 1 cut(s) 322
Hsp92I GRCGYC 2 cut(s) 42, 60
Hsp92II CATG 1 cut(s) 956
Kzo9I GATC 5 cut(s) 102, 156, 232, 551, 622
LmnI GCTCC 1 cut(s) 796
Lsp1109I GCAGC 3 cut(s) 283, 777, 859
LweI GCATC 2 cut(s) 135, 346
MaeI CTAG 1 cut(s) 48
MaeII ACGT 1 cut(s) 322
MaeIII GTNAC 2 cut(s) 442, 656
MalI GATC 5 cut(s) 104, 158, 234, 553, 624
MbiI CCGCTC 1 cut(s) 329
MboI GATC 5 cut(s) 102, 156, 232, 551, 622
MboII GAAGA 6 cut(s) 112, 266, 386, 415, 475, 889
MflI RGATCY 1 cut(s) 102
MhlI GDGCHC 1 cut(s) 893
MlsI TGGCCA 1 cut(s) 395
MluCI AATT 6 cut(s) 73, 208, 471, 512, 851, 869
MluNI TGGCCA 1 cut(s) 395
MlyI GAGTC 2 cut(s) 17, 413
MmeI TCCRAC 1 cut(s) 581
Mox20I TGGCCA 1 cut(s) 395
MscI TGGCCA 1 cut(s) 395
MseI TTAA 3 cut(s) 485, 540, 786
Msp20I TGGCCA 1 cut(s) 395
MspCI CTTAAG 1 cut(s) 484
MspI CCGG 1 cut(s) 794
MspR9I CCNGG 1 cut(s) 16
MvaI CCWGG 1 cut(s) 16
MwoI GCNNNNNNNGC 2 cut(s) 532, 796
NdeII GATC 5 cut(s) 102, 156, 232, 551, 622
NlaIII CATG 1 cut(s) 956
NlaIV GGNNCC 1 cut(s) 760
PfeI GAWTC 2 cut(s) 466, 682
PkrI GCNGC 5 cut(s) 53, 56, 273, 792, 849
PleI GAGTC 2 cut(s) 17, 413
PpsI GAGTC 2 cut(s) 17, 413
Psp6I CCWGG 1 cut(s) 14
PspGI CCWGG 1 cut(s) 14
PspN4I GGNNCC 1 cut(s) 760
PspPI GGNCC 1 cut(s) 119
PsrI GAACNNNNNNTAC 2 cut(s) 756, 788
PstI CTGCAG 1 cut(s) 879
PstNI CAGNNNCTG 1 cut(s) 428
PsuI RGATCY 1 cut(s) 102
RsaI GTAC 2 cut(s) 248, 584
RsaNI GTAC 2 cut(s) 247, 583
SaqAI TTAA 3 cut(s) 485, 540, 786
SatI GCNGC 5 cut(s) 52, 55, 272, 791, 848
Sau3AI GATC 5 cut(s) 102, 156, 232, 551, 622
Sau96I GGNCC 1 cut(s) 119
SchI GAGTC 2 cut(s) 17, 413
ScrFI CCNGG 1 cut(s) 16
SduI GDGCHC 1 cut(s) 893
SfaNI GCATC 2 cut(s) 135, 346
SfcI CTRYAG 3 cut(s) 561, 592, 875
SmlI CTYRAG 1 cut(s) 484
SmoI CTYRAG 1 cut(s) 484
Sse9I AATT 6 cut(s) 73, 208, 471, 512, 851, 869
SsiI CCGC 5 cut(s) 27, 51, 54, 327, 838
SspMI CTAG 1 cut(s) 48
StyD4I CCNGG 1 cut(s) 14
StyI CCWWGG 3 cut(s) 138, 193, 447
TaaI ACNGT 2 cut(s) 562, 711
TaiI ACGT 1 cut(s) 325
TaqI TCGA 1 cut(s) 588
TasI AATT 6 cut(s) 73, 208, 471, 512, 851, 869
TatI WGTACW 1 cut(s) 246
TauI GCSGC 2 cut(s) 54, 57
TfiI GAWTC 2 cut(s) 466, 682
Tru1I TTAA 3 cut(s) 485, 540, 786
Tru9I TTAA 3 cut(s) 485, 540, 786
TscAI CASTG 2 cut(s) 256, 837
TseI GCWGC 3 cut(s) 271, 790, 847
TspGWI ACGGA 1 cut(s) 732
TspRI CASTG 2 cut(s) 256, 837
Vha464I CTTAAG 1 cut(s) 484
XapI RAATTY 3 cut(s) 471, 512, 869
XmiI GTMKAC 1 cut(s) 300
XspI CTAG 1 cut(s) 48
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.